A traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot

Abstract

The present invention relates to the technical field of fertilizer preparation, and in particular to a traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot. The composite microbial fertilizer for inhibiting angelica root rot provided by the present invention comprises 45-55 parts of composite fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of a composite microbial agent, and 8-12 parts of a traditional Chinese medicine extract. The composite microbial agent comprises Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride, and Trichoderma harzianum, with a mass ratio of 14-18:12-16:11-15:8-12:8-12:5-9:5-9, each containing 400-600 million bacteria per gram. The traditional Chinese medicine extract comprises Scutellaria baicalensis, Ephedra sinica, Tagetes erecta, Nerium oleander, Ginkgo biloba, and Sophora flavescens. This fertilizer not only prevents and treats angelica root rot but also significantly improves the yield and quality of angelica, achieving excellent results.

Description

[0001] This application is a divisional application. The application number of the original application is: 202410774851.5, the application date is 2024.06.17, and the name of the invention is: A composite microbial fertilizer for inhibiting angelica root rot and its preparation method. Technical Field

[0002] The invention relates to the technical field of fertilizer preparation, in particular to a traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot. Background Art

[0003] Angelica sinensis (Oliv.) Diels is a perennial herbaceous plant in the Apiaceae family. Its roots are cylindrical, branched, and yellow-brown. Its stems are erect, greenish-white or purple with deep grooves. Its leaves are pinnately divided, purple or green, and ovate. Its flowers are white, with densely pubescent pedicels, long ovate petals, and conical stylopodiums. It blooms from June to July. Its fruits are oval to ovate, with pale purple wing margins, and fruit from July to September. The Compendium of Materia Medica states: "In ancient times, people married to continue their family line. Angelica sinensis regulates blood circulation and is an essential medicine for women, symbolizing longing for their husbands, hence the name 'Danggui.'"

[0004] Nowadays, the demand for angelica is increasing, and the area of ​​angelica cultivation is constantly expanding, resulting in a shortening of the rotation cycle, which has led to a significant increase in the incidence of angelica diseases, among which root rot is particularly prominent.

[0005] Due to the long-standing prevalence and rapid onset of root rot pathogens, root rot remains a major cause of yield and quality decline in angelica sinensis. Chemical control agents can effectively inhibit the growth of the pathogen, but excessive use poses serious risks to the environment and human health. Using environmentally friendly plant-based probiotics to inhibit the growth of root rot pathogens is an important production method and a key trend in the prevention and control of angelica sinensis root rot, promoting the sustainable development of traditional Chinese medicine.

[0006] Therefore, how to provide a microbial preparation for preventing and treating Angelica root rot is a problem that those skilled in the art urgently need to solve. Summary of the Invention

[0007] The present invention aims to provide a composite microbial fertilizer for inhibiting angelica root rot and a preparation method thereof.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The present invention provides a composite microbial fertilizer for inhibiting angelica root rot, which is prepared from the following components in parts by mass: 45-55 parts of compound fertilizer, 20-26 parts of wood ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of composite bacterial agent, and 8-12 parts of traditional Chinese medicine extract;

[0010] The composite bacterial agent includes: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum;

[0011] The Chinese medicinal extracts include: scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens.

[0012] Preferably, the mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15.

[0013] Preferably, the mass ratio of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9.

[0014] Preferably, the bacterial content of the Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 400 million to 600 million per gram.

[0015] Preferably, the mass ratio of the scutellaria baicalensis, ephedra, marigold, oleander, ginkgo biloba and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7.

[0016] Preferably, the preparation method of the traditional Chinese medicine extract is: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens to obtain a mixture; mixing the mixture with water, boiling the mixture, and filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0017] Preferably, the mass ratio of the mixture to water is 1:5-7; and the decocting time is 3-5 hours.

[0018] The present invention also provides a method for preparing the composite microbial fertilizer, which comprises mixing the composite bacterial agent, wood ash, compound fertilizer, humic acid, diatomaceous earth and traditional Chinese medicine extract.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] Among the components added in the present invention, the composite bacterial agent can effectively prevent and control root rot in angelica. Bacillus licheniformis, Bacillus cereus, Pseudomonas campestris, and Pseudomonas chlororaphis can effectively prevent and control Fusarium. Trichoderma viride and Trichoderma harzianum can prevent and control root-knot nematodes, and Trichoderma viride can also prevent and control Pseudomonas syringae. Bacillus atrophaeus can prevent and control Rhizoctonia. Therefore, the bacterial species added in the present invention take into account the various pathogenic factors of root rot and have a good control effect on various root rot pathogens, effectively addressing the impact of complex field environments on the growth of angelica.

[0021] Among the traditional Chinese medicine extracts added by the present invention, scutellaria baicalensis has a significant inhibitory effect on the spore germination of pathogenic bacteria, ephedra has a significant inhibitory effect on the growth of mycelium of pathogenic bacteria, and marigold, oleander, ginkgo leaf and sophora flavescens have excellent effects on killing pathogens. The above traditional Chinese medicine components are used in combination to effectively prevent and control different developmental stages of pathogens, and can also effectively prevent the infection and spread of root rot pathogens.

[0022] The composite microbial fertilizer provided by the present invention can effectively prevent and treat angelica root rot, can be applied as base fertilizer during land preparation, and does not require topdressing. It can also significantly improve the yield and quality of angelica, and has multiple uses with excellent effects. DETAILED DESCRIPTION

[0023] The invention provides a composite microbial fertilizer for inhibiting root rot of angelica sinensis. The composite microbial fertilizer is prepared from the following components in parts by mass: 45-55 parts of compound fertilizer, 20-26 parts of wood ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of composite bacterial agent and 8-12 parts of traditional Chinese medicine extract; preferably, the composite fertilizer is 47-53 parts of compound fertilizer, 21-25 parts of wood ash, 7-9 parts of humic acid, 4-6 parts of diatomaceous earth, 5-7 parts of composite bacterial agent and 9-11 parts of traditional Chinese medicine extract; further preferably, the composite fertilizer is 49-51 parts of compound fertilizer, 22-24 parts of wood ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of composite bacterial agent and 10 parts of traditional Chinese medicine extract; and more preferably, the composite fertilizer is 50 parts of compound fertilizer, 23 parts of wood ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of composite bacterial agent and 10 parts of traditional Chinese medicine extract.

[0024] The composite bacterial agent includes: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum;

[0025] The Chinese medicinal extracts include: scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens.

[0026] In the present invention, the mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15; preferably 17-21:6-8:12-14; more preferably 18-20:7:13; more preferably 19:7:1.

[0027] In the present invention, the mass ratio of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9; preferably 15-17:13-15:12-14:9-11:9-11:6-8:6-8; further preferably 16:14:13:10:10:7:7.

[0028] In the present invention, the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 400 million to 600 million / g, preferably 500 million / g.

[0029] In the present invention, the mass ratio of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7; preferably 11-15:14-16:8-10:9-11:5-7:4-6; further preferably 12-14:15:9:10:6:5; more preferably 13:15:9:10:6:5.

[0030] In the present invention, the preparation method of the traditional Chinese medicine extract is: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens to obtain a mixed material; mixing the mixed material with water, boiling the mixture, and filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0031] In the present invention, the mass ratio of the mixed material to water is 1:5-7, preferably 1:6.

[0032] In the present invention, the decocting time is 3 to 5 hours, preferably 4 hours.

[0033] The present invention also provides a method for preparing the composite microbial fertilizer, which comprises mixing the composite bacterial agent, wood ash, compound fertilizer, humic acid, diatomaceous earth and traditional Chinese medicine extract.

[0034] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0035] Example 1

[0036] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0037] Mix 45 parts of compound fertilizer, 20 parts of wood ash, 6 parts of humic acid, 3 parts of diatomaceous earth, 4 parts of compound bacterial agent and 8 parts of Chinese herbal medicine extract;

[0038] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 14:12:11:8:8:5:5; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 400 million / g;

[0039] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16:5:11;

[0040] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens in a mass ratio of 10:13:7:8:4:3 to obtain a mixed material; mixing the mixed material with water in a mass ratio of 1:5 and boiling for 3 hours; filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0041] Example 2

[0042] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0043] Mix 55 parts of compound fertilizer, 26 parts of wood ash, 10 parts of humic acid, 7 parts of diatomaceous earth, 8 parts of compound bacterial agent and 12 parts of Chinese herbal medicine extract;

[0044] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 18:16:15:12:12:9:9; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 600 million / g;

[0045] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 22:9:15;

[0046] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens in a mass ratio of 16:17:11:12:8:7 to obtain a mixed material; mixing the mixed material with water in a mass ratio of 1:7 and boiling for 5 hours; filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0047] Example 3

[0048] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0049] Mix 50 parts of compound fertilizer, 23 parts of wood ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of compound bacterial agent and 10 parts of Chinese herbal medicine extract;

[0050] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 16:14:13:10:10:7:7; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 500 million / g;

[0051] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 19:7:13;

[0052] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens in a mass ratio of 13:15:9:10:6:5 to obtain a mixed material; mixing the mixed material with water in a mass ratio of 1:6 and boiling for 4 hours; and filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0053] Experimental Example 1

[0054] In Anning District, Lanzhou, a plot of land was selected for the cultivation of angelica sinensis (Danggui gui 2). Aside from fertilizer application, all other management methods were identical for each experimental group. All groups received only basal fertilizer, with no topdressing. The soil was covered with plastic film, and the row spacing of the angelica seedlings was 0.3 m × 0.4 m. Two replicates were set up for each experimental group. The results were averaged.

[0055] The product obtained by the method of Example 3 was used as the experimental group; the method of use was: apply 60 kg / mu as a base fertilizer at one time during land preparation;

[0056] Control group 1: other methods were the same as those in Example 3, except that Bacillus atrophaeus and Pseudomonas chlororaphis were not added; the application method was: 60 kg / mu was applied as a base fertilizer during land preparation;

[0057] Control group 2: other methods were the same as those in Example 3, except that no Trichoderma viride and Trichoderma harzianum were added; the method of use was: a one-time application of 60 kg / mu as base fertilizer during land preparation;

[0058] Control group 3: other methods were the same as those in Example 3, except that Trichoderma viride and Trichoderma harzianum were replaced with equal amounts of Bacillus subtilis and Bacillus megaterium; the method of use was: a one-time application of 60 kg / mu as base fertilizer during land preparation;

[0059] Control group 4: other methods were the same as those in Example 3, except that scutellaria baicalensis and ephedra were not added; the method of use was: a one-time application of 60 kg / mu as base fertilizer during land preparation;

[0060] Control group 5: other methods were the same as those in Example 3, except that marigold and oleander were replaced with equal amounts of bletilla striata and purslane; the method of use was: a one-time application of 60 kg / mu as base fertilizer during land preparation;

[0061] Existing technology group: Referring to the control agent described in Example CN 201710615476.X, root irrigation was performed at the early stage of angelica root rot, at a dosage of 25 kg / mu.

[0062] The incidence of angelica root rot was counted during the peak period of its occurrence. The yield of angelica was counted after harvest, and the ferulic acid content and volatile oil content of the angelica were measured. The results are shown in Tables 1 to 3.

[0063] Incidence rate = (number of diseased plants / total number of plants) × 100%

[0064] The ferulic acid content was determined by high performance liquid chromatography, and the volatile oil determination method was based on the method 2204B of the General Rules of Part IV of the 2020 edition of the Chinese Pharmacopoeia.

[0065] Table 1 Incidence of Angelica root rot

[0066] Incidence % Experimental group 5.8 Control group 1 9.6 Control group 2 10.4 Control group 3 7.9 Control group 4 10.9 Control group 5 8.7 Existing Technology Group 14.7

[0067] Table 2 Yield of fresh roots of Angelica sinensis

[0068]

[0069]

[0070] Table 3 Ferulic acid and volatile oil content

[0071] Ferulic acid % Volatile oil% Experimental group 0.131 0.534 Control group 1 0.123 0.472 Control group 2 0.125 0.471 Control group 3 0.128 0.510 Control group 4 0.125 0.468 Control group 5 0.127 0.498 Existing Technology Group 0.116 0.423

[0072] It can be seen from the contents recorded in Tables 1 to 3 that the bacterial fertilizer provided by the present invention has excellent technical effects in preventing and treating root rot, and can also significantly improve the yield and quality of angelica sinensis, and its use effect is significantly better than that of existing products.

[0073] It can be seen from the data of control groups 1 to 3 that the strains used in the present invention act synergistically with each other. If some of the components are discarded or replaced with other strains with similar efficacy, the technical effects of the present invention cannot be achieved, which has a significant impact on the fertilizer efficiency.

[0074] It can be seen from the data of control groups 4 and 5 that the Chinese medicinal components used in the present invention also act synergistically with each other. If some of the components are discarded or replaced with other strains with similar medicinal effects, the technical effects of the present invention cannot be achieved, which has a significant impact on the fertilizer efficiency.

[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A composite microbial fertilizer for inhibiting root rot of angelica sinensis, characterized in that: The invention is prepared from the following components by weight: 45-55 parts of compound fertilizer, 20-26 parts of wood ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of compound bacterial agent and 8-12 parts of traditional Chinese medicine extract; The composite bacterial agent is composed of: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum; The Chinese herbal medicine extract is composed of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo biloba and sophora flavescens; the preparation method of the Chinese herbal medicine extract is: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo biloba and sophora flavescens to obtain a mixed material; mixing the mixed material with water, boiling, and filtering to obtain an extract, which is the Chinese herbal medicine extract; The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15; The mass ratio of the Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9; The bacterial contents of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum are all 400 million to 600 million per gram.

2. The composite microbial fertilizer according to claim 1, characterized in that The mass ratio of the scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7.

3. The composite microbial fertilizer according to claim 1, characterized in that The mass ratio of the mixed material to water is 1:5-7; and the decoction time is 3-5 hours.

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