A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs and its preparation method

Abstract

The invention provides a safflower oil compound preparation for improving skin inflammation of pet cats and dogs and its preparation method, the preparation method is as follows: preparation of safflower oil microcapsules; preparation of deep-sea fish oil microcapsules; preparation of chitosan solution as cortex solution; taking safflower oil microcapsules, deep-sea fish oil microcapsules and water, stirring evenly as core layer solution; using ethanol as coagulation bath, wet spinning is carried out to obtain chitosan fibers coated with safflower oil microcapsules and deep-sea fish oil microcapsules; drying, short cutting, and obtaining. The present invention adopts a fiber structure with safflower oil microcapsules and deep-sea fish oil microcapsules loaded internally at the same time. The microcapsules are temperature-responsive. After cats and dogs eat, they enter the oral cavity and assist in chewing. The microcapsules rupture, and safflower oil and deep-sea fish oil are released and mixed in the fiber. Under anaerobic conditions, their oxidation rate is not affected. After entering the stomach, under gastric acid, the outer fiber (pH-responsive type) dissolves, and the oil dissolves, and begins to exert its effect.

Description

Technical Field

[0001] The present invention specifically relates to a safflower seed oil compound preparation for improving skin inflammation of pet cats and dogs and a preparation method thereof. Background Art

[0002] Safflower, a herbaceous plant in the Asteraceae family, is a versatile economic crop with diverse uses, including oil, medicine, beverages, and natural pigments. Safflower seed oil, also known as safflower oil, is a clear yellow liquid vegetable oil refined from safflower seeds through pressing and other extraction processes. It is a specialized substance that combines medicinal, oil, and feed properties. Safflower seed oil is recognized worldwide for its edible, health, and beauty benefits. Analysis and identification reveal that the main components of safflower seed oil are palmitic acid (6%-8%), oleic acid (16%-20%), stearic acid (2%-3%), and linoleic acid (73%-85%). Safflower seed oil also contains various active ingredients, including natural vitamin E and flavonoids. This has earned it the nicknames "King of Linoleic Acid" and "Crown of Vitamin E." Safflower seed oil has been shown to lower blood lipids and blood sugar, and has antioxidant, anti-aging, and anti-inflammatory properties. Wang Ren'ai studied the anti-inflammatory effects of safflower seed oil using ear and toe swelling experiments on mice. The results showed that the swelling rate in the treated group was significantly reduced compared to the model group, demonstrating a significant anti-inflammatory effect. However, in practice, safflower seed oil contains a particularly high content of linoleic acid (ω-6 fatty acid). Upon entering the intestine, it is first converted into arachidonic acid, which then attaches to cell membranes and secretes metabolites, stimulating inflammation. Studies have also shown that consuming large amounts of linoleic acid can cause inflammation and increase the risk of ulcerative colitis. Therefore, how to balance the linoleic acid content in safflower seed oil to achieve an optimal anti-inflammatory effect is an urgent issue addressed in this patent. Summary of the Invention

[0003] Technical problem to be solved: In response to the above technical problems, the purpose of the present invention is to provide a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs and a preparation method thereof. The fiber structure is loaded with safflower seed oil microcapsules and deep-sea fish oil microcapsules. The microcapsules are temperature-responsive. After cats and dogs eat, they enter the mouth (temperature 38-39°C) and assist in chewing. The microcapsules rupture, and the safflower seed oil and deep-sea fish oil are released and mixed in the fiber. The oxidation rate is not affected under anaerobic conditions. After entering the stomach, the outer layer of fiber (pH-responsive) dissolves under gastric acid, and the oil is dissolved and begins to exert its efficacy.

[0004] Technical solution: A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. The safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs is a fiber that is loaded with safflower seed oil microcapsules and deep-sea fish oil microcapsules at the same time; the material of the fiber is a pH-responsive material, and the membrane of the microcapsule is a temperature-responsive membrane.

[0005] Furthermore, the pH response value of the pH responsive material is ≤5.

[0006] Furthermore, the temperature response value of the temperature responsive membrane is ≥37.7°C.

[0007] Furthermore, the deep-sea fish oil includes salmon oil, sardine oil, tuna oil and mackerel oil.

[0008] The preparation method of the safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0009] (1) Preparation of safflower oil microcapsules: Take modified gelatin, dissolve it in deionized water, add safflower oil, and homogenize at a speed of 12000-15000 rpm to obtain a safflower oil crude emulsion. Ultrasonicate for 2-3 min to obtain a safflower oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain safflower oil microcapsules.

[0010] (2) Preparation of deep-sea fish oil microcapsules: Take modified gelatin, dissolve it in deionized water, add deep-sea fish oil, homogenize at a speed of 13500-16000 rpm to obtain a deep-sea fish oil crude emulsion, ultrasonicate for 2-3 min to obtain a deep-sea fish oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain deep-sea fish oil microcapsules;

[0011] (3) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 3-5% as the cortical solution;

[0012] (4) Take safflower seed oil microcapsules, deep-sea fish oil microcapsules and water, stir them evenly, and use them as the core layer solution;

[0013] (5) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules;

[0014] (6) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs.

[0015] Furthermore, the preparation method of the modified gelatin is as follows:

[0016] Step 1: Prepare a gelatin aqueous solution at a concentration of 20 mg / mL and adjust the pH to 9.00.

[0017] Step 2: Prepare a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjust the pH to 9.00;

[0018] Step 3: Mix equal volumes of the gelatin aqueous solution and the grape seed polyphenol aqueous solution, and stir at 36°C and 200-300 rpm for 20-24 hours;

[0019] Step 4: Place in a dialysis bag with a molecular weight cut-off of 8000-14000Da and dialyze for 48 hours, changing ultrapure water every 6 hours;

[0020] Step 5: Freeze-dry to obtain modified gelatin.

[0021] Furthermore, in step (1), the mass ratio of modified gelatin, deionized water and safflower oil is 1:50:(0.5-0.8).

[0022] Furthermore, in step (2), the mass ratio of modified gelatin, deionized water and deep-sea fish oil is 1:45:(0.4-0.7).

[0023] Furthermore, in step (4), the ratio of safflower seed oil microcapsules to deep-sea fish oil microcapsules is 1:(3-5).

[0024] Furthermore, the chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules can be twisted to form a twisted shape and then chopped. Beneficial effects

[0025] Since the linoleic acid content in safflower seed oil is particularly high, as high as 73%-85%, when the linoleic acid (ω-6 fatty acid) content is particularly high, it is easy to induce a super-physiological inflammatory response, causing chronic inflammation to persist for a long time, which has the effect of aggravating inflammation. Comparative Example 3 of the present invention also proves that its effect when used alone is not good. Therefore, the present invention uses safflower seed oil and deep-sea fish oil for compounding. Deep-sea fish oil is rich in ω-3 fatty acids. After the two are compounded, a better ω-6 / ω-3 ratio is achieved, thereby improving its anti-inflammatory effect.

[0026] The present invention microencapsulates safflower seed oil and deep-sea fish oil separately, thereby solving the problem that direct mixing of safflower seed oil and deep-sea fish oil accelerates the oxidation process, reduces antioxidant stability, and reduces the effect thereof. Furthermore, cats and dogs are very sensitive to odors, and direct mixing of safflower seed oil and deep-sea fish oil produces a special odor that causes them to reject eating. However, microencapsulation can mask the odor and does not affect their normal eating.

[0027] The present invention adopts a fiber structure with safflower seed oil microcapsules and deep-sea fish oil microcapsules loaded inside. The microcapsules are temperature-responsive. After cats and dogs eat, they enter the mouth (temperature 38-39°C) and assist in chewing. The microcapsules rupture, and the safflower seed oil and deep-sea fish oil are released and mixed in the fiber. Their oxidation rate is not affected under anaerobic conditions. After entering the stomach, the outer layer of fiber (pH-responsive) dissolves under gastric acid, and the oil dissolves and begins to exert its efficacy.

[0028] The present invention uses grape seed polyphenol to modify gelatin. On the one hand, it can improve the response temperature and pH stability of gelatin. It is stable below the normal room temperature of 35°C, and can begin to disintegrate and dissolve oil at the oral temperature of cats and dogs (38-39°C). On the other hand, grape seed polyphenol can also improve the antioxidant properties of gelatin and better protect oil.

[0029] The fiber is prepared from chitosan as a raw material. Chitosan has good antibacterial properties, dissolves in gastric acid, and can effectively improve the storage stability of oils and fats. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 13 and Comparative Example 4 show the change in peroxide value of the oil over time. DETAILED DESCRIPTION

[0031] The present invention provides a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs and its application. To make the purpose, technical solution, and effects of the present invention clearer and more specific, the present invention will be further described in detail with reference to the following examples. It should be understood that the specific embodiments described herein are only intended to illustrate the present invention and are not intended to limit the present invention. Example 1

[0032] The preparation method of modified gelatin is as follows:

[0033] Step 1: Prepare a gelatin aqueous solution at a concentration of 20 mg / mL and adjust the pH to 9.00.

[0034] Step 2: Prepare a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjust the pH to 9.00;

[0035] Step 3: Mix equal volumes of the gelatin aqueous solution and the grape seed polyphenol aqueous solution, and stir at 36°C and 250 rpm for 24 hours;

[0036] Step 4: Place in a dialysis bag with a molecular weight cut-off of 8000-14000Da and dialyze for 48 hours, changing ultrapure water every 6 hours;

[0037] Step 5: Freeze-dry to obtain modified gelatin.

[0038] In dynamic rheological property measurements, the melting turning point is generally defined as tan(δ) = 1, and the corresponding temperature is the melting temperature. In dynamic viscoelastic analysis, the melting temperatures can be determined separately. The melting temperature of unmodified gelatin was measured at 35.7°C, while that of modified gelatin was 37.3°C, an increase of 1.6°C. Example 2

[0039] Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.5g of safflower oil, homogenize at a speed of 14000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 3

[0040] Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 14000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 4

[0041] Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.8g of safflower oil, homogenize at a speed of 14000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 5

[0042] Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 12000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Example 6

[0043] Preparation of safflower oil microcapsules: Take 1g of modified gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 15000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules. Comparative Example 1

[0044] The difference between this embodiment and embodiment 3 is that unmodified gelatin is used, specifically as follows:

[0045] Preparation of safflower oil microcapsules: Take 1g of gelatin, dissolve it in 50g of deionized water, add 0.7g of safflower oil, homogenize at a speed of 14000rpm to obtain a safflower oil crude emulsion, ultrasonicate for 2 minutes to obtain a safflower oil emulsion, put it in a -40℃ refrigerator to freeze, freeze-dry, and sieve to obtain safflower oil microcapsules.

[0046] The average particle size, oil embedding efficiency and safflower oil melting temperature of the safflower oil microcapsules in the above embodiment were measured. The results are shown in Table 1 below:

[0047] Table 1

[0048] Average particle size (nm) Embedding rate (%) Safflower oil melting temperature (℃) Example 2 311.4 92.67 37.7 Example 3 313.5 93.42 37.8 Example 4 314.9 93.49 37.8 Example 5 343.7 93.19 37.7 Example 6 298.3 93.26 37.8 Comparative Example 1 347.5 90.94 36.0

[0049] After comparison of comprehensive performance, the safflower seed oil microcapsules prepared in Example 3 were selected for subsequent experiments. Example 7

[0050] Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.4g of salmon oil, homogenize at a speed of 15000rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules. Example 8

[0051] Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 15000rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules. Example 9

[0052] Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.7g of salmon oil, homogenize at a speed of 15000rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules. Example 10

[0053] Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 13500rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules. Example 11

[0054] Preparation of salmon oil microcapsules: Take 1g of modified gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 16000rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules. Comparative Example 2

[0055] The difference between this embodiment and embodiment 8 is that unmodified gelatin is used, specifically as follows:

[0056] Preparation of salmon oil microcapsules: Take 1g of gelatin, dissolve it in 45g of deionized water, add 0.6g of salmon oil, homogenize at a speed of 15000rpm to obtain a salmon oil crude emulsion, ultrasonicate for 2.5 minutes to obtain a salmon oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain salmon oil microcapsules.

[0057] The average particle size, oil embedding efficiency and salmon oil melting temperature of the salmon oil microcapsules of the above examples were measured. The results are shown in Table 2 below:

[0058] Table 2

[0059] Average particle size (nm) Embedding rate (%) Salmon oil melting temperature (℃) Example 7 313.7 93.44 37.7 Example 8 315.6 94.13 37.8 Example 9 316.8 94.02 37.8 Example 10 322.1 93.67 37.7 Example 11 302.2 94.05 37.8 Comparative Example 2 355.5 90.28 36.1

[0060] After comparison of comprehensive performance, the salmon oil microcapsules prepared in Example 8 were selected for subsequent experiments. Example 12

[0061] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0062] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 3%, which was used as the cortical solution.

[0063] (2) Take 1g of safflower oil microcapsules, 3g of salmon oil microcapsules and 50mL of water, stir evenly and use them as the core layer solution;

[0064] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0065] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 13

[0066] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0067] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 4%, which was used as the cortical solution.

[0068] (2) Take 1g of safflower oil microcapsules, 3g of salmon oil microcapsules and 50mL of water, stir evenly and use them as the core layer solution;

[0069] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0070] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 14

[0071] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0072] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 5%, which was used as the cortical solution;

[0073] (2) Take 1g of safflower oil microcapsules, 3g of salmon oil microcapsules and 50mL of water, stir evenly and use them as the core layer solution;

[0074] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0075] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 15

[0076] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0077] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 4%, which was used as the cortical solution.

[0078] (2) Take 1g of safflower oil microcapsules, 2g of salmon oil microcapsules and 50mL of water, stir evenly, and use them as the core layer solution;

[0079] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0080] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Example 16

[0081] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0082] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 4%, which was used as the cortical solution.

[0083] (2) Take 1g of safflower oil microcapsules, 4g of salmon oil microcapsules and 50mL of water, stir evenly and use them as the core layer solution;

[0084] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0085] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs. Comparative Example 3

[0086] The difference between this embodiment and embodiment 13 is that all safflower seed oil microcapsules are used, specifically as follows:

[0087] A method for preparing a safflower seed oil preparation comprises the following steps:

[0088] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 4%, which was used as the cortical solution.

[0089] (2) Take 3 g of safflower seed oil microcapsules and 50 mL of water, stir evenly, and use them as the core layer solution;

[0090] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower seed oil microcapsules;

[0091] (4) Dry and cut into pieces of 1-2 mm to obtain safflower seed oil preparation. Comparative Example 4

[0092] The difference between this embodiment and embodiment 13 is that safflower seed oil / salmon oil mixed microcapsules are used, and the mass ratio of safflower seed oil / salmon oil is consistent with that of embodiment 13, specifically as follows:

[0093] A method for preparing a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs comprises the following steps:

[0094] (1) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 4%, which was used as the cortical solution.

[0095] (2) Take 4 g of safflower oil / salmon oil mixed microcapsules and 50 mL of water, stir evenly, and use as the core layer solution;

[0096] (3) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower oil microcapsules and salmon oil microcapsules;

[0097] (4) Dry and cut into pieces of 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs.

[0098] Preparation of safflower oil / salmon oil mixed microcapsules: Take modified gelatin, dissolve it in deionized water, add safflower oil and salmon oil, and homogenize at a speed of 14500 rpm to obtain a safflower oil / salmon oil crude emulsion. Ultrasonicate for 2.5 minutes to obtain a safflower oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain safflower oil / salmon oil microcapsules.

[0099] The ratio of ω-6 unsaturated fatty acids to ω-3 polyunsaturated fatty acids in the safflower seed oil compound preparations for improving skin inflammation in pet cats and dogs obtained in the above-mentioned embodiments was determined; (1) Determination of the oxidative stability index (OSI): determination was performed using an oil oxidation stability tester at a temperature of 110°C and an air flow rate of 20 L / h; (2) Determination of the initial DPPH free radical scavenging rate: 0.2 g of oil sample was mixed with 0.75 mL of methanol, vortexed for 1 min, and the supernatant was taken for testing. 0.21 mL of the test solution was added with 0.7 mL of DPPH-ethanol solution (0.1 mmol / L), vortexed and mixed, and then reacted at 25°C in the dark for 30 min, and the absorbance was measured at 517 nm; (3) Determination of the initial ABTS free radical scavenging rate: 0.30 mL of the test solution was added with 0.18 μL of a working solution (composed of 7 mmol / L ABTS solution and 2.45 mmol / L K 2 The S2O8 solution was prepared by reacting at 25°C in the dark for 30 min, and the absorbance was measured at 734 nm. The results are shown in Table 3 below:

[0100] Table 3

[0101]

[0102] From the comparative example 4 in the above table, it can be seen that after safflower seed oil and salmon oil are mixed, their oxidative stability index, DPPH free radical scavenging rate and ABTS free radical scavenging rate are all reduced. Therefore, the early mixing of oils and fats will accelerate oxidation. Therefore, early mixing is not recommended.

[0103] The storage stability of Example 12, Example 13, and Comparative Example 4 was measured. Each example was stored at 20-25° C., and the dissolution time of safflower oil and salmon oil from the microcapsules and the dissolution time from the fibers were measured at different times. The results are shown in Table 4 below.

[0104] Table 4

[0105]

[0106] The peroxide values ​​(POV) of Example 13 and Comparative Example 4 were measured. The peroxide value was determined according to GB 5009.227-2023, Determination of Peroxide Value of Animal and Vegetable Oils. In Example 13, the safflower oil and salmon oil were measured separately before the oils were dissolved from the microcapsules. After the oils were dissolved from the microcapsules, the mixed oils were measured. The results are as follows: Figure 1 shown.

[0107] Study on the therapeutic effect of safflower seed oil compound preparation on improving skin inflammation in pet cats and dogs:

[0108] The 24 sick dogs and cats were admitted to an animal hospital with clinical symptoms such as hair loss, erythema, scabs, papules, etc. and were diagnosed with skin diseases, including 12 dogs and 12 cats.

[0109] Dogs and cats were divided into 3 groups, 8 in each group. The safflower seed oil compound preparations for improving skin inflammation in pet cats and dogs prepared in Example 13, Comparative Example 3 and Comparative Example 4 were added to the diet, with a dosage of 0.88 g / kg body weight / d for cats and 0.92 g / kg body weight / d for dogs, and the feeding was continued for 30 days.

[0110] A scoring system for clinical symptom severity in dogs and cats was established. Three veterinarians scored the severity of skin lesions in the 24 dogs and cats participating in the study before and after treatment, with the average of the three scores used as the skin lesion score. The scoring system evaluated the severity of hair loss, erythema, crusting, and papules at 16 different body sites (Table 5) using a grading system with 0 points for no symptoms, 1 point for mild symptoms, 2 points for moderate symptoms, and 3 points for severe symptoms.

[0111] Table 5

[0112] Serial number Part Serial number Part Serial number Part 1 face 7 groin 13 left hind limb 2 head 8 abdomen 14 right hind limb 3 ears 9 waist 15 perianal area 4 neck 10 flank 16 tail 5 armpits 11 Left forelimb 6 Chest 12 Right forelimb

[0113] The pets involved in the study included six female dogs, six male dogs, six female cats, and six male cats. 75% of the pets had hair loss, 54% had erythema, 50% had scabs, and 42% had papules.

[0114] The skin lesion scores before and after treatment are shown in Table 6 below, where No. 1-8 uses the safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs prepared in Example 13; No. 9-16 uses the safflower seed oil preparation prepared in Comparative Example 3; and No. 17-24 uses the safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs prepared in Comparative Example 4:

[0115] Table 6

[0116]

[0117] Efficacy evaluation criteria:

[0118] Efficacy evaluation criteria for erythema, hair loss, papules, and crusting were established. The efficacy index (E) was calculated as the pre-treatment score minus the post-treatment score / pre-treatment score × 100%. Efficacy index: 100% ≥ E > 75% is excellent; 75% ≥ E > 50% is good; 50% ≥ E > 25% is fair; and 25% ≥ E is ineffective. Efficacy evaluation is shown in Table 7 below:

[0119] Table 7

[0120]

[0121] Adverse reaction records:

[0122] The dogs and cats were observed daily and the number of cases with symptoms such as vomiting, diarrhea, lethargy, loss of appetite, polydipsia, and polyuria during treatment was recorded.

[0123] Among the 24 sick dogs and cats participating in the trial, cat No. 14 had slight vomiting, and the other sick dogs and cats showed no adverse reactions during treatment.

Claims

1. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs, characterized by: The safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs is a fiber loaded with safflower seed oil microcapsules and deep-sea fish oil microcapsules; the fiber is made of a pH-responsive material, and the membrane of the microcapsule is a temperature-responsive membrane; The preparation method comprises the following steps: (1) Preparation of safflower oil microcapsules: Take modified gelatin, dissolve it in deionized water, add safflower oil, and homogenize at a speed of 12000-15000 rpm to obtain a safflower oil crude emulsion. Ultrasonicate for 2-3 min to obtain a safflower oil emulsion, freeze it in a -40°C refrigerator, freeze-dry it, and sieve it to obtain safflower oil microcapsules. (2) Preparation of deep-sea fish oil microcapsules: Take modified gelatin, dissolve it in deionized water, add deep-sea fish oil, homogenize at a speed of 13500-16000 rpm to obtain a deep-sea fish oil crude emulsion, ultrasonicate for 2-3 min to obtain a deep-sea fish oil emulsion, freeze it in a -40℃ refrigerator, freeze-dry it, and sieve it to obtain deep-sea fish oil microcapsules; (3) Chitosan was dissolved in 1% acetic acid solution and glycerol was added to obtain a chitosan solution with a mass fraction of 3-5% as the cortical solution; (4) Take safflower seed oil microcapsules, deep-sea fish oil microcapsules and water, stir them evenly, and use them as the core layer solution; (5) Using ethanol as a coagulation bath, the cortex solution and the core solution were wet-spun at a spinning flow rate of 50 mL / h for the cortex and 80 mL / h for the core to obtain chitosan fibers coated with safflower seed oil microcapsules and deep-sea fish oil microcapsules; (6) Drying and chopping into 1-2 mm to obtain a safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs; The preparation method of the modified gelatin is as follows: Step 1: Prepare a gelatin aqueous solution at a concentration of 20 mg / mL and adjust the pH to 9.

00. Step 2: Prepare a grape seed polyphenol aqueous solution with a concentration of 4 mg / mL and adjust the pH to 9.00; Step 3: Mix equal volumes of the gelatin aqueous solution and the grape seed polyphenol aqueous solution, and stir at 36°C and 200-300 rpm for 20-24 hours; Step 4: Place in a dialysis bag with a molecular weight cut-off of 8000-14000Da and dialyze for 48 hours, changing ultrapure water every 6 hours; Step 5: Freeze-dry to obtain modified gelatin.

2. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The pH response value of the pH responsive material is ≤5.

3. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The temperature response value of the temperature responsive membrane is ≥37.7°C.

4. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: The deep-sea fish oil includes salmon oil, sardine oil, tuna oil and mackerel oil.

5. A safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: In step (1), the mass ratio of modified gelatin, deionized water and safflower oil is 1:50:(0.5-0.8).

6. The safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: In step (2), the mass ratio of modified gelatin, deionized water and deep-sea fish oil is 1:45:(0.4-0.7).

7. The safflower seed oil compound preparation for improving skin inflammation in pet cats and dogs according to claim 1, characterized in that: In step (4), the ratio of safflower seed oil microcapsules to deep-sea fish oil microcapsules is 1:(3-5).

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