Fluorescent probe containing pyrene group, preparation method and application

By designing a fluorescent probe containing pyrene groups, using the mechanism of electrostatic action and host-guest complex formation, high selectivity and high sensitivity detection of protamine, trypsin and α-amylase are achieved, solving the problem of difficult detection in the prior art.

CN119684149AActive Publication Date: 2025-03-25BEIJING TECH & BUSINESS UNIV
3 Cites 0 Cited by

Patent Information

Application Number
CN202411854528.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-16
Publication Date
2025-03-25
Estimated Expiration
2044-12-16

AI Technical Summary

Technical Problem

Existing fluorescent probes are difficult to achieve high selectivity and high sensitivity detection of protamine, trypsin and α-amylase.

Method used

A fluorescent probe containing pyrene groups was designed to bind to protamine through electrostatic action and form a host-guest complex with γ-cyclodextrin to achieve quantitative detection of these biological macromolecules.

Benefits of technology

It has achieved high selectivity and high sensitivity detection of protamine, trypsin and α-amylase, and has the potential for application in complex media.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a fluorescent probe containing a pyrene group as well as a preparation method and application of the fluorescent probe. The fluorescent probe disclosed by the invention is a ratio type fluorescent molecule, and is combined with protamine through electrostatic interaction to induce emission of excimer. Protamine can be hydrolyzed by adding trypsin, and fluorescence recovery monomer emission is realized, so that trypsin detection is realized. In addition, the probe and gamma-cyclodextrin form a host-guest compound system, and excimer fluorescence emission is shown. Based on the hydrolysis effect of alpha-amylase on cyclodextrin, fluorescence recovers monomer emission, and quantitative detection of alpha-amylase is realized. The invention provides a multifunctional fluorescence detection probe which has good selectivity and sensitivity and can be applied to a serum dilution environment.
Need to check novelty before this filing date? Find Prior Art