Method for extracting momordica grosvenori saponin V from momordica grosvenori

Abstract

The invention relates to a method for extracting momordica grosvenori saponin V from momordica grosvenori, which can solve the problems of tedious process, time and material waste, high production cost, low product content and low yield caused by generation of an acid-base solution in the intermediate process in the existing method. The method comprises the following steps: crushing and pulping a siraitia grosvenorii raw material to obtain slurry; uniformly mixing the slurry and purified water according to a mass-volume ratio of 1g: (4-6) mL to obtain a mixed material liquid; adding the mixed material liquid into a secondary coil pipeline of magnetic induction electric field extraction equipment, filling the secondary coil pipeline with the mixed material liquid, and performing circular flow extraction on the mixed material liquid in a magnetic induction electric field to obtain an extracting solution; adding an organic solvent and an inorganic salt solution, stirring, standing for layering, and concentrating the upper phase solution to obtain a concentrated solution; performing electrodialysis desalting and debitterizing treatment on the concentrated solution until the conductivity of the desalting chamber is lower than 100 [mu] s / cm to obtain an electrodialysis solution; and performing concentration and vacuum drying on the decolored solution in sequence to obtain the mogroside V. The invention also discloses a preparation method of the mogroside V.

Description

Technical Field

[0001] The invention relates to a method for extracting chemical substances from natural substances, and in particular to a method for extracting mogroside V from momordica grosvenori. Background Art

[0002] The nutritional value of monk fruit is extremely high. It contains a large amount of carbohydrates, proteins, vitamins, fatty acids, a variety of essential amino acids and trace elements for the human body.

[0003] Mogroside is the main active ingredient in monk fruit. It is used in traditional Chinese medicine to treat colds, coughs, sore throats, gastrointestinal discomfort and other symptoms. Mogroside also has blood-purifying, antioxidant, anti-diabetic and anti-cancer activities. The composition of mogrosides in fresh monk fruit of different fruit ages is different. Starting from the 10th day after pollination, as the fruit age increases, the content of mogroside II gradually decreases to undetectable, the content of mogroside III first gradually increases, and then gradually decreases to undetectable, mogroside V begins to appear on the 50th day, as the fruit age increases, the content of mogroside V gradually increases, the content of mogroside IV shows an increasing trend from the 30th to the 50th day, and begins to decrease sharply after the 50th day. From the 70th day onwards, the content of mogroside V reaches a high level, mogroside II and mogroside III are undetectable, and the content of mogroside IV is very low. Among them, mogroside V is a triterpene glucoside, whose glycoside is triterpene alcohol, a cucurbitacinane-type compound, and a non-sugar sweetener, which is nearly 300 times sweeter than sucrose. Therefore, as the most important sweet substance and active ingredient in monk fruit, the preparation of mogroside V has received attention from many scholars at home and abroad.

[0004] The Chinese patent with publication number CN101690573A discloses "a method for producing a monk fruit extract with a content of mogroside V greater than 60%". The method uses fresh monk fruit through the process of: crushing → saccharification → water extraction → concentration → sedimentation centrifugation → ion exchange resin refining → macroporous resin refining → concentration → alumina refining → concentration → spray drying → finished product to obtain a mogroside V with a content of 60%.

[0005] A Chinese patent with publication number CN103923152B discloses "a method for extracting mogroside V". The method sequentially obtains a mogroside V extract with a final product content of 60% through saccharification of fresh monk fruit, water extraction and concentration, sedimentation centrifugation, macroporous resin adsorption separation, ion exchange resin refining, ethanol concentration and recovery, silica gel refining, concentration and spray drying.

[0006] A Chinese patent with publication number CN107629105A discloses "a method for purifying flavorful mogroside V". The method sequentially undergoes polyamide adsorption, alkali adjustment and ultrafiltration, ion exchange adsorption, decolorization and drying to obtain flavorful mogroside V. The mass content of mogroside V is greater than 60%, and the yield is ≥90%.

[0007] The Chinese patent with publication number CN115109112B discloses "an industrial production method of mogroside V for increasing the content of mogroside V". The method sequentially comprises crushing fresh monk fruit, water extraction, filtration clarification, gradient treatment with resin, concentration and spray drying to obtain mogroside V with a content of about 50%.

[0008] The Chinese patent with publication number CN113854533B discloses "a method for preparing mogroside, mogrophenolic acid, mogroside concentrated juice and mogrosine from fresh monk fruit". The method first uses a pulsed electric field method to assist in crushing the fresh monk fruit, and then sequentially performs water extraction, ceramic membrane clarification, ion exchange resin separation and purification, macroporous adsorption resin purification, polystyrene-based reverse phase gel purification, edible oil deplasticizer and ceramic membrane clarification treatment to obtain mogroside V with a content of >50%. This process is too complicated and difficult to operate in practice.

[0009] However, the common problems of the above existing methods are: complicated procedures, time-consuming and material-consuming, high production cost, low product content and low yield due to the generation of acidic and alkaline solutions in the intermediate process. Summary of the invention

[0010] The purpose of the present invention is to solve the technical problems of the existing method, such as complicated procedures, time-consuming and material-consuming, high production cost, low product content and low yield due to the generation of acidic and alkaline solutions in the intermediate process, and to provide a method for extracting mogroside V from monk fruit.

[0011] In order to achieve the above object, the present invention adopts the following technical solution:

[0012] A method for extracting mogroside V from Momordica grosvenori is characterized in that it comprises the following steps:

[0013] Step 1: Crushing and magnetic induction electric field extraction

[0014] Step 1.1, crushing and pulping the monk fruit raw material to obtain a slurry; the monk fruit raw material is a mature fresh monk fruit;

[0015] Step 1.2, the slurry and purified water are mixed evenly at a mass volume ratio of 1g:4-6mL to obtain a mixed liquid; the mixed liquid is added to the secondary coil pipeline of the magnetic induction electric field extraction equipment so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid is circulated and extracted in the magnetic induction electric field provided by the secondary coil pipeline, and filtered to obtain an extract;

[0016] Step 2: Aqueous Two-Phase Extraction

[0017] Adding an organic solvent and an inorganic salt solution to the extract to form a two-phase aqueous extraction system, stirring, standing and separating the layers, taking the upper phase solution and concentrating it to obtain a concentrated solution, and adding the lower phase solution to the next batch of extracts;

[0018] Step 3: Electrodialysis purification

[0019] The concentrated solution is added into the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is performed until the conductivity of the desalination chamber is lower than 100 μs / cm, and the treatment is stopped to obtain an electrodialysis solution;

[0020] Step 4: Decolorization

[0021] The electrodialysis liquid is decolorized and filtered, and then the decolorized liquid is concentrated and vacuum dried in sequence to obtain white mogroside V.

[0022] Further, step 1.1 specifically comprises: using a shell-breaking machine to crush and pulp the monk fruit raw material to obtain a slurry of shell, seed and juice; the monk fruit raw material is a fresh monk fruit that has been left after ripening; after a period of ripening, the color of the shell of the monk fruit turns yellow, the water in the fruit evaporates naturally and saccharification is carried out inside, and the content of mogroside V in the obtained ripe monk fruit fresh fruit increases, providing high-quality raw materials for the subsequent extraction of high-content mogroside V;

[0023] In order to fully extract mogroside V, in step 1.2, the mixed liquid is pumped into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 20-40 ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates and flows in the magnetic induction electric field provided by the secondary coil pipeline for extraction. The reaction frequency of the magnetic induction electric field is 60-75 kHz, the excitation voltage is 600-800 V, the extraction temperature is 65-75 ° C, and the extraction time is 40-60 min.

[0024] Further, in order to fully remove impurities in the extract, step 2 is specifically as follows: adding an inorganic salt solution and an organic solvent with a concentration of 95% to the extract to form a two-phase extraction system, wherein the concentration of the organic solvent in the two-phase extraction system is 25%-40%, and the concentration of the inorganic salt is 22%-28%, and then stirring at 20-30°C for 20-40min, standing for 1-2h and then separating the layers, taking the upper phase solution, and concentrating it to a specific gravity of 1.01-1.03g / cm under the conditions of a vacuum degree of -0.08 to -0.06MPa and a temperature of 65-75°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0025] Furthermore, in order to increase the content, step 3 is specifically as follows: adding the concentrated solution into the desalination chamber of the electrodialysis system, and then performing electrodialysis desalination and debittering treatment for 0.5-1.5h at an operating voltage of 20-35V, a flow rate of 10-20L / h, and a temperature of 25-35°C, until the conductivity of the desalination chamber is lower than 100μs / cm, stopping the treatment, and obtaining an electrodialysis solution.

[0026] Further, in order to improve the quality, step 4 is specifically as follows: adding activated carbon in an amount of 1% to 3% of the mass of the electrodialysis liquid to the electrodialysis liquid, stirring and decolorizing for 40-90 minutes at a temperature of 60-80°C, filtering, and then concentrating and vacuum-drying the decolorized liquid in sequence at a temperature of 65-75°C and a vacuum degree of -0.08 to -0.06MPa to obtain white mogroside V.

[0027] Furthermore, in step 2, the inorganic salt solution is ammonium sulfate solution, magnesium sulfate solution, sodium carbonate solution or dipotassium hydrogen phosphate solution; the organic solvent is methanol or ethanol;

[0028] In step 4, the activated carbon is 320 activated carbon, Senhuan activated carbon, 767 activated carbon or KF03 activated carbon.

[0029] Furthermore, step 1.2 is specifically as follows: the slurry is evenly mixed with purified water at a mass volume ratio of 1g:5mL to obtain a mixed liquid; the mixed liquid is pumped into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 30ml / min using a peristaltic pump of the magnetic induction electric field extraction equipment to fill the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; the reaction frequency of the magnetic induction electric field is 70kHz, the excitation voltage is 720V, the extraction temperature is 70°C, and the extraction time is 50min.

[0030] Further, step 2 is specifically as follows: adding ammonium sulfate solution and 95% ethanol to the extract to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 30% and the concentration of ammonium sulfate is 26%, and then stirring at 25°C for 30 minutes, standing for 1.5 hours and then separating the layers, taking the upper phase solution, and concentrating it to a specific gravity of 1.02 g / cm under the conditions of a vacuum degree of -0.08 to -0.06 MPa and a temperature of 70°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0031] Furthermore, step 3 is specifically as follows: adding the concentrated solution into the desalination chamber of the electrodialysis system, and then performing electrodialysis desalination and debittering treatment for 1 hour at an operating voltage of 24V, a flow rate of 15L / h, and a temperature of 28°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysis solution.

[0032] Furthermore, step 4 is specifically: adding 2% of Senhuan activated carbon to the electrodialysis solution, stirring and decolorizing for 60 minutes at a temperature of 70°C, filtering, and then concentrating and vacuum-drying the decolorized liquid at a temperature of 70°C and a vacuum degree of -0.08 to -0.06MPa to obtain white mogroside V.

[0033] Beneficial effects of the present invention:

[0034] 1. The present invention provides a method for extracting mogroside V from Momordica grosvenori. The method sequentially performs induction electric field extraction, aqueous two-phase extraction, electrodialysis desalting and debittering, and decolorization, and the content and yield of the obtained mogroside V are greatly improved. In addition, the method of the present invention has simple procedures and low production costs.

[0035] 2. In the present invention, after the monk fruit raw material is crushed and pulped, purified water is used as a medium for mixing, and then added to the magnetic induction electric field extraction equipment, and the soluble monk fruit saponin V in the mixed liquid is extracted using the induced alternating electric field. This extraction method takes a short time and requires fewer extraction times. The thermal effect generated by the induced electric field can achieve a rapid extraction effect. The extraction efficiency is much higher than that of the existing extraction method, and it is safe and green, does not introduce other exogenous substances, and has mild reaction conditions.

[0036] 3. In the present invention, the aqueous two-phase extraction method is used to extract the mogroside V in the extract. Compared with the traditional macroporous adsorption resin purification process, the advantages of the aqueous two-phase extraction method are: large processing capacity, low energy consumption, short extraction time, high recovery rate, a large amount of impurities can enter the salt phase and be removed, the separation effect is good, the salt phase can be used for the next batch of aqueous two-phase extraction after treatment, and the operation is simple and easy to operate continuously.

[0037] 4. In the present invention, the concentrated solution is desalted and debittered by electrodialysis, and no other chemical reagents are introduced, so that the obtained mogroside V can be guaranteed to have higher purity and recovery rate. This method has low pollution, less other pollutants are generated during the desalting and debittering process, the process is relatively simple, stable and reliable, and can avoid the acid-base reaction generated by the traditional ion exchange resin method, solving the problem of low recovery rate of mogroside V after debittering and desalting by the ion exchange resin method.

[0038] 5. In the present invention, the conditions of the entire extraction and purification process are mild, and the solvents used are ethanol, methanol and purified water, which can reduce the flammable and explosive risk factors of other organic reagents in the experimental process and are more environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 The HPLC chromatogram of the mogroside V obtained in Example 1 of the method for extracting mogroside V from momordica grosvenori of the present invention. DETAILED DESCRIPTION

[0040] Embodiment 1

[0041] Step 1: Crushing and magnetic induction electric field extraction

[0042] Step 1.1, using a shelling machine to mechanically crush and pulp 1 kg of monk fruit raw material to obtain a pulp of shell, seeds and juice; wherein the monk fruit raw material is fresh monk fruit that has been left after ripening, and through small sample extraction and testing, the content of mogroside V in the raw material is about 0.7%-0.9%;

[0043] Step 1.2, the slurry obtained in step 1.1 is mixed evenly with purified water in a mass volume ratio of 1g:5mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 30ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; wherein the reaction frequency of the magnetic induction electric field is 70kHz, the excitation voltage is 720V, the extraction temperature is 70℃, and the extraction time is 50min.

[0044] Step 2: Aqueous Two-Phase Extraction

[0045] Add ammonium sulfate solution and 95% ethanol to the extract obtained in step 1.2 to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 30% and the concentration of ammonium sulfate is 26%, and then stir at 25°C for 30 minutes, stand for 1.5 hours, separate the layers, take the upper phase solution, and concentrate it to a specific gravity of 1.02 g / cm under the conditions of vacuum degree of -0.07 MPa and temperature of 70°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0046] Step 3: Electrodialysis purification

[0047] The concentrated solution obtained in step 2 is added to the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 1 hour at an operating voltage of 24V, a flow rate of 15L / h, and a temperature of 28°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysate.

[0048] Step 4: Decolorization

[0049] To the electrodialysate obtained in step 3, add Senhuan activated carbon with a mass of 2% of the electrodialysate, stir and decolorize for 60 min at a temperature of 70° C., filter, and then concentrate and vacuum-dry the decolorized liquid at a temperature of 70° C. and a vacuum degree of -0.07 MPa to obtain white mogroside V, with a content of 71.82% and a yield of 95.9%. The HPLC chromatogram of mogroside V is as shown in FIG. Figure 1 As shown, Figure 1 The corresponding HPLC chromatographic detection data are shown in Table 1:

[0050] Table 1

[0051] Element Retention time / min Peak area / μV*sec Peak height / μV content / % Mogroside V 17.833 571256 16358 71.82

[0052] Embodiment 2

[0053] Step 1: Crushing and magnetic induction electric field extraction

[0054] Step 1.1, using a shelling machine to mechanically crush and pulp 1 kg of monk fruit raw material to obtain a pulp of shell, seeds and juice; wherein the monk fruit raw material is fresh monk fruit that has been left after ripening, and through small sample extraction and testing, the content of mogroside V in the raw material is about 0.7%-0.9%;

[0055] Step 1.2, the slurry obtained in step 1.1 is mixed evenly with purified water in a mass volume ratio of 1g:4mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 25ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; wherein the reaction frequency of the magnetic induction electric field is 65kHz, the excitation voltage is 760V, the extraction temperature is 70℃, and the extraction time is 50min.

[0056] Step 2: Aqueous Two-Phase Extraction

[0057] Add ammonium sulfate solution and 95% ethanol to the extract obtained in step 1.2 to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 27% and the concentration of ammonium sulfate is 26%, and then stir at 25°C for 30 minutes, stand for 1.5 hours, separate the layers, take the upper phase solution, and concentrate it to a specific gravity of 1.01 g / cm under the conditions of vacuum degree of -0.06 MPa and temperature of 70°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0058] Step 3: Electrodialysis purification

[0059] The concentrated solution obtained in step 2 is added to the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 1 hour at an operating voltage of 26V, a flow rate of 14L / h, and a temperature of 28°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysate.

[0060] Step 4: Decolorization

[0061] Add 767 activated carbon with a mass of 1% of the electrodialysate to the electrodialysate obtained in step 3, stir and decolorize for 60 min at a temperature of 70° C., filter, and then concentrate and vacuum-dry the decolorized solution at a temperature of 70° C. and a vacuum degree of -0.06 MPa to obtain white mogroside V, with a content of 70.94% and a yield of 93.3%. The HPLC chromatogram of mogroside V is shown in Table 1. Figure 1 .

[0062] Embodiment 3

[0063] Step 1: Crushing and magnetic induction electric field extraction

[0064] Step 1.1, using a shelling machine to mechanically crush and pulp 1 kg of monk fruit raw material to obtain a pulp of shell, seeds and juice; wherein the monk fruit raw material is fresh monk fruit that has been left after ripening, and through small sample extraction and testing, the content of mogroside V in the raw material is about 0.7%-0.9%;

[0065] Step 1.2, the slurry obtained in step 1.1 is mixed evenly with purified water in a mass volume ratio of 1g:6mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 20ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; wherein the reaction frequency of the magnetic induction electric field is 60kHz, the excitation voltage is 800V, the extraction temperature is 65°C, and the extraction time is 50min.

[0066] Step 2: Aqueous Two-Phase Extraction

[0067] Add magnesium sulfate solution and 95% ethanol to the extract obtained in step 1.2 to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 30% and the concentration of magnesium sulfate is 28%, and then stir at 25°C for 30 minutes, stand for 1.5 hours, separate the layers, take the upper phase solution, and concentrate it to a specific gravity of 1.03 g / cm under the conditions of vacuum degree of -0.07 MPa and temperature of 70°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0068] Step 3: Electrodialysis purification

[0069] The concentrated solution obtained in step 2 is added to the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 1 hour at an operating voltage of 20V, a flow rate of 12L / h, and a temperature of 25°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysate.

[0070] Step 4: Decolorization

[0071] Add 320 activated carbon with a mass of 3% of the electrodialysate to the electrodialysate obtained in step 3, stir and decolorize for 60 minutes at a temperature of 70°C, filter, and then concentrate and vacuum dry the decolorized liquid at a temperature of 70°C and a vacuum degree of -0.07MPa to obtain white mogroside V. The content of mogroside V is 71.05%, and the yield of mogroside V is 96.9%. The HPLC chromatogram of mogroside V is shown in Table 1. Figure 1 .

[0072] Embodiment 4

[0073] Step 1: Crushing and magnetic induction electric field extraction

[0074] Step 1.1, using a shelling machine to mechanically crush and pulp 1 kg of monk fruit raw material to obtain a pulp of shell, seeds and juice; wherein the monk fruit raw material is fresh monk fruit that has been left after ripening, and through small sample extraction and testing, the content of mogroside V in the raw material is about 0.7%-0.9%;

[0075] Step 1.2, the slurry obtained in step 1.1 is mixed evenly with purified water at a mass volume ratio of 1g:5mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 33ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; wherein the reaction frequency of the magnetic induction electric field is 75kHz, the excitation voltage is 600V, the extraction temperature is 75℃, and the extraction time is 60min.

[0076] Step 2: Aqueous Two-Phase Extraction

[0077] Sodium carbonate solution and 95% ethanol were added to the extract obtained in step 1.2 to form a two-phase extraction system, in which the concentration of ethanol was 40% and the concentration of sodium carbonate was 28%, and then stirred at 20°C for 40 minutes, and separated after standing for 1 hour. The upper phase solution was taken and concentrated to a specific gravity of 1.02 g / cm under the conditions of vacuum degree of -0.08 MPa and temperature of 75°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0078] Step 3: Electrodialysis purification

[0079] The concentrated solution obtained in step 2 is added to the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 1.5 hours at an operating voltage of 35V, a flow rate of 10L / h, and a temperature of 26°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysate.

[0080] Step 4: Decolorization

[0081] To the electrodialysate obtained in step 3, add KF03 activated carbon with a mass of 1% of the electrodialysate, stir and decolorize for 40 min at a temperature of 80° C., filter, and then concentrate and vacuum-dry the decolorized solution at a temperature of 75° C. and a vacuum degree of -0.08 MPa to obtain white mogroside V, with a content of 71.27% and a yield of 94.3%. The HPLC chromatogram of mogroside V is shown in Table 1. Figure 1 .

[0082] Embodiment 5

[0083] Step 1: Crushing and magnetic induction electric field extraction

[0084] Step 1.1, using a shelling machine to mechanically crush and pulp 1 kg of monk fruit raw material to obtain a pulp of shell, seeds and juice; wherein the monk fruit raw material is fresh monk fruit that has been left after ripening, and through small sample extraction and testing, the content of mogroside V in the raw material is about 0.7%-0.9%;

[0085] Step 1.2, the slurry obtained in step 1.1 is mixed evenly with purified water in a mass volume ratio of 1g:5mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 40ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; wherein the reaction frequency of the magnetic induction electric field is 70kHz, the excitation voltage is 720V, the extraction temperature is 70℃, and the extraction time is 40min.

[0086] Step 2: Aqueous Two-Phase Extraction

[0087] Add dipotassium hydrogen phosphate solution and 95% methanol to the extract obtained in step 1.2 to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 25% and the concentration of dipotassium hydrogen phosphate is 22%, and then stir at 30°C for 20 minutes, stand for 2 hours, separate the layers, take the upper phase solution, and concentrate it to a specific gravity of 1.02 g / cm under the conditions of vacuum degree of -0.06 MPa and temperature of 65°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

[0088] Step 3: Electrodialysis purification

[0089] The concentrated solution obtained in step 2 is added to the desalting chamber of the electrodialysis system, and then the electrodialysis desalting and debittering treatment is carried out for 0.5h at an operating voltage of 24V, a flow rate of 20L / h, and a temperature of 35°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain an electrodialysate.

[0090] Step 4: Decolorization

[0091] To the electrodialysate obtained in step 3, add KF03 activated carbon with a mass of 2% of the electrodialysate, stir and decolorize for 90 min at a temperature of 60° C., filter, and then concentrate and vacuum-dry the decolorized solution at a temperature of 65° C. and a vacuum degree of -0.06 MPa to obtain white mogroside V, with a content of 70.46% and a yield of 96.4% for mogroside V. The HPLC chromatogram of mogroside V is shown in FIG. Figure 1 .

[0092] It should be noted that the magnetic induction electric field extraction equipment in Examples 1 to 5 is a MFR-2L product produced by Indust (Wuxi) Induction Technology Co., Ltd. The magnetic induction electric field extraction equipment includes an inlet, a peristaltic pump, a U-shaped silicon steel chip, a primary coil, a secondary coil pipeline, an outlet, an excitation power supply and other components. The working principle of the magnetic induction electric field extraction equipment is as follows: a peristaltic pump is used at the inlet to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment, so that the mixed liquid fills the entire secondary coil pipeline. When working, due to the excitation signal of the variable frequency power supply, it is applied to the primary coil. According to the working principle of the transformer, the secondary coil pipeline generates a magnetic induction electric field, and the mixed liquid circulates and flows in the magnetic induction electric field for extraction.

[0093] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for extracting mogroside V from Momordica grosvenori, characterized in that: The following steps are involved: Step 1: Crushing and magnetic induction electric field extraction Step 1.1, crushing and pulping the monk fruit raw material to obtain a slurry; the monk fruit raw material is a mature fresh monk fruit; Step 1.2, the slurry and purified water are mixed evenly at a mass volume ratio of 1g:4-6mL to obtain a mixed liquid; the mixed liquid is added to the secondary coil pipeline of the magnetic induction electric field extraction equipment so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid is circulated and extracted in the magnetic induction electric field provided by the secondary coil pipeline, and filtered to obtain an extract; Step 2: Aqueous Two-Phase Extraction Adding an organic solvent and an inorganic salt solution to the extract to form a two-phase aqueous extraction system, stirring, standing and separating the layers, taking the upper phase solution and concentrating it to obtain a concentrated solution, and adding the lower phase solution to the next batch of extracts; Step 3: Electrodialysis purification The concentrated solution is added into the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is performed until the conductivity of the desalination chamber is lower than 100 μs / cm, and the treatment is stopped to obtain an electrodialysis solution; Step 4: Decolorization The electrodialysis liquid is decolorized and filtered, and then the decolorized liquid is concentrated and vacuum dried in sequence to obtain white mogroside V.

2. A method for extracting mogroside V from Momordica grosvenori according to claim 1, characterized in that: Step 1.1 specifically comprises: using a shelling machine to crush and pulp the monk fruit raw material to obtain a pulp of shells, seeds and juice; the monk fruit raw material is fresh monk fruit that has been ripened and left to stand; In step 1.2, the mixed liquid is pumped into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 20-40 ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates and flows in the magnetic induction electric field provided by the secondary coil pipeline for extraction. The reaction frequency of the magnetic induction electric field is 60-75 kHz, the excitation voltage is 600-800 V, the extraction temperature is 65-75 ° C, and the extraction time is 40-60 min.

3. A method for extracting mogroside V from Momordica grosvenori according to claim 2, characterized in that: Step 2 is as follows: An inorganic salt solution and an organic solvent with a concentration of 95% are added to the extract to form a two-phase aqueous extraction system, wherein the concentration of the organic solvent in the two-phase aqueous extraction system is 25%-40%, and the concentration of the inorganic salt is 22%-28%. The extract is then stirred at 20-30° C. for 20-40 minutes, allowed to stand for 1-2 hours, and then separated into layers. The upper phase solution is taken and concentrated to a specific gravity of 1.01-1.03 g / cm under the conditions of a vacuum degree of -0.08 to -0.06 MPa and a temperature of 65-75° C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

4. A method for extracting mogroside V from Momordica grosvenori according to claim 3, characterized in that: Step 3 is as follows: The concentrated solution is added into the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 0.5-1.5h at an operating voltage of 20-35V, a flow rate of 10-20L / h, and a temperature of 25-35°C until the conductivity of the desalination chamber is lower than 100μs / cm, and the treatment is stopped to obtain the electrodialysis solution.

5. A method for extracting mogroside V from Momordica grosvenori according to claim 4, characterized in that: Step 4 is as follows: Add activated carbon in an amount of 1% to 3% of the mass of the electrodialysis liquid to the electrodialysis liquid, stir and decolorize for 40 to 90 minutes at a temperature of 60 to 80° C., filter, and then concentrate and vacuum-dry the decolorized liquid at a temperature of 65 to 75° C. and a vacuum degree of -0.08 to -0.06 MPa to obtain white mogroside V.

6. A method for extracting mogroside V from Momordica grosvenori according to claim 5, characterized in that: In step 2, the inorganic salt solution is ammonium sulfate solution, magnesium sulfate solution, sodium carbonate solution or dipotassium hydrogen phosphate solution; the organic solvent is methanol or ethanol; In step 4, the activated carbon is 320 activated carbon, Senhuan activated carbon, 767 activated carbon or KF03 activated carbon.

7. A method for extracting mogroside V from Momordica grosvenori according to claim 2, characterized in that: Step 1.2 is as follows: The slurry is mixed evenly with purified water in a mass volume ratio of 1g:5mL to obtain a mixed liquid; the peristaltic pump of the magnetic induction electric field extraction equipment is used to pump the mixed liquid into the secondary coil pipeline of the magnetic induction electric field extraction equipment at a flow rate of 30ml / min, so that the mixed liquid fills the entire secondary coil pipeline, and the mixed liquid circulates and flows in the magnetic induction electric field provided by the secondary coil pipeline for extraction and filtration to obtain an extract; the reaction frequency of the magnetic induction electric field is 70kHz, the excitation voltage is 720V, the extraction temperature is 70°C, and the extraction time is 50min.

8. A method for extracting mogroside V from Momordica grosvenori according to claim 6, characterized in that: Step 2 is as follows: Add ammonium sulfate solution and 95% ethanol to the extract to form a two-phase extraction system, wherein the concentration of ethanol in the two-phase extraction system is 30% and the concentration of ammonium sulfate is 26%, and then stir at 25°C for 30 minutes, stand for 1.5 hours, separate the layers, take the upper phase solution, and concentrate it to a specific gravity of 1.02 g / cm under the conditions of vacuum degree of -0.08 to -0.06 MPa and temperature of 70°C. 3 , a concentrated solution is obtained, and the lower phase solution is used to add to the next batch of extracts.

9. A method for extracting mogroside V from Momordica grosvenori according to claim 8, characterized in that: Step 3 is as follows: The concentrated solution is added into the desalination chamber of the electrodialysis system, and then the electrodialysis desalination and debittering treatment is carried out for 1 hour under the conditions of operating voltage of 24V, flow rate of 15L / h and temperature of 28°C. When the conductivity of the desalination chamber is lower than 100μs / cm, the treatment is stopped to obtain the electrodialysis liquid.

10. The method for extracting mogroside V from Momordica grosvenori according to claim 9, characterized in that: Step 4 is as follows: Add 2% of Senhuan activated carbon to the electrodialysis liquid, stir and decolorize for 60 minutes at a temperature of 70°C, filter, and then concentrate and vacuum dry the decolorized liquid at a temperature of 70°C and a vacuum degree of -0.08 to -0.06 MPa to obtain white mogroside V.

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