Preparation method of acenaphthene or fluorene artificial antigen
Through the coupling of Fuke acylation reaction and activated ester reaction to the carrier protein, a high-potent aceta or fluorene antigen is prepared, which solves the problem of aceta or fluorene detection in the prior art and achieves a high sensitivity and accuracy detection effect.
Patent Information
- Application Number
- CN202411856198.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-17
- Publication Date
- 2025-03-25
AI Technical Summary
The prior art is difficult to effectively detect aceta or fluorene, mainly because these polycyclic aromatic hydrocarbons are not reactive and immunogenic themselves and lack active groups, which limit their direct coupling to carrier proteins and their ability to induce specific antibodies in animals.
Acetogen or fluorene antigen with high titer is prepared by coupling with carrier proteins through the Fuker acylation reaction and the activated ester reaction. The specific steps include acylation reaction of acetosine or fluorene with compounds such as aluminum trichloride and succinic anhydride, then adding a coupling agent and a polar solvent for activation, and finally coupling reaction with the carrier protein.
The efficient synthesis of aceta or fluorene artificial antigen is achieved, the coupling efficiency and specificity with carrier proteins is improved, specific antibodies can be induced in animals, and the sensitivity and accuracy of detection are improved.