Multi-channel myocardial in-vitro perfusion system

Abstract

The invention discloses a multichannel myocardial in-vitro perfusion system, and relates to the technical field of perfusion, the multichannel myocardial in-vitro perfusion system comprises a support frame and a perfusion assembly, the perfusion assembly is arranged on the support frame, and the perfusion assembly further comprises a plurality of temperature control tubes which are transversely distributed at intervals and fixedly connected with the support frame; the temperature control flow guide assembly is arranged in the temperature control pipe, the temperature control flow guide assembly further comprises a flow guide column fixedly connected with the temperature control pipe, a transversely-extending and through flow guide groove is formed in the flow guide column, a perfusion groove communicated with the flow guide groove is formed in the flow guide column, and baffles are hinged to the two ends in the flow guide groove through rotating shafts. The adjusting assembly is arranged in the flow guide column and used for adjusting the blocking state of the baffle, and the constant temperature assembly is arranged on the outer side of the perfusion assembly and used for keeping the temperature of fluid in the perfusion assembly; according to the invention, high efficiency, diversity and accuracy of experimental results during perfusion experiments can be ensured.

Description

Technical Field

[0001] The invention relates to the technical field of perfusion, and in particular to a multi-channel myocardial in vitro perfusion system. Background Art

[0002] At present, the isolated heart perfusion system is an important tool for studying the electrophysiological activity of the isolated heart and is widely used in various fields of medicine, including research fields in basic and clinical related departments such as physiology, pharmacology, and biochemistry. The system inserts a heart cannula into the aorta under constant temperature and constant flow conditions, and retrogradely perfuses oxygenated perfusion fluid (such as KH solution) to maintain the normal physiological activity of the isolated heart in the outside world.

[0003] However, traditional systems usually only have a single channel and are inefficient, making it difficult to meet the experimental needs of simultaneously studying the effects of multiple drugs or different conditions on the isolated heart. At the same time, during the use of the perfusion system, although a constant temperature circuit is set up to heat and keep the perfusion fluid warm, the constant temperature circuit cannot be quickly constructed in the early stage of the experiment, resulting in the perfusion fluid failing to reach a constant temperature during the early flow process. At this time, after the perfusion fluid flows into the isolated heart, it not only fails to maintain the external physiological activities of the isolated heart, but also easily affects the accuracy of the experimental results. It also affects the activity of the isolated heart, which is not conducive to the stable progress of the experiment.

[0004] Application number CN201810426958.5 discloses an isolated heart perfusion experimental device, including a heart perfusion device with a perfusion catheter and an indwelling needle, the indwelling needle includes a puncture needle and a catheter with openings at both ends, the rear end of the catheter is fixed with a fixed joint for detachably connecting with the liquid outlet end of the perfusion catheter, the fixed joint is provided with an interface communicating with the inner cavity of the catheter, and the front end of the puncture needle passes through the interface and the inner cavity of the catheter in turn. Using this isolated heart perfusion experimental device is conducive to the operator quickly hanging the heart removed from the experimental mouse on the heart perfusion device, so as to ensure the activity of the removed heart. However, when the perfusion fluid flows in the early stage, the patent has a greater impact on the isolated heart due to the large temperature change, which not only causes heart fibrillation, but may even cause a certain degree of damage to the living body.

[0005] Therefore, a multi-channel myocardial in vitro perfusion system is invented to solve the above problems. Summary of the invention

[0006] The main purpose of the present invention is to provide a multi-channel myocardial in vitro perfusion system, which can effectively solve the technical problems in the background technology.

[0007] To achieve the above object, the technical solution adopted by the present invention is: a multi-channel myocardial in vitro perfusion system, including a support frame, and also including: A perfusion assembly, which is arranged on the support frame and is used to perfuse the isolated myocardium, and the perfusion assembly also includes a plurality of temperature control tubes which are distributed at intervals in the transverse direction and fixedly connected to the support frame; A temperature control flow guide component, the temperature control flow guide component is arranged in the temperature control tube, the temperature control flow guide component also includes a flow guide column fixedly connected to the temperature control tube, a flow guide groove extending transversely and penetrating therein is provided in the flow guide column, a perfusion groove connected to the flow guide groove is provided on the flow guide column, baffles are hinged at both ends of the flow guide groove through a rotating shaft, and when one of the baffles blocks the flow guide groove, the other baffle no longer blocks the flow guide groove; An adjusting component, the adjusting component is arranged inside the guide column and is used to adjust the blocking state of the baffle; A constant temperature component is arranged outside the perfusion component and is used to maintain the temperature of the fluid in the perfusion component.

[0008] Preferably, the adjustment component includes a receiving groove opened on the guide column, a piston cylinder with closed two ends fixedly connected in the receiving groove, a piston plate slidably connected in the piston cylinder, air pipes are connected at both ends of the piston cylinder, and adjustment boxes are fixedly connected at both ends of the receiving groove, the free end of the air pipe is connected to the corresponding adjustment box, the end of the rotating shaft close to the receiving groove passes through the corresponding adjustment box, and an adjustment plate slidably connected to the inside of the adjustment box is fixedly connected to the rotating shaft.

[0009] Preferably, a push block is fixedly connected to the piston plate, and one end of the push block away from the piston plate extends out of the piston cylinder and is slidably connected to the piston cylinder. A guide groove is provided on the end surface of the push block away from the piston plate, and a push rod is slidably connected in the accommodating groove. The free end of the push rod is fixedly connected to an insertion rod, and the insertion rod is inserted into the guide groove. When the push rod slides, the push block slides back and forth along the axial direction of the piston cylinder under the action of the guide groove.

[0010] Preferably, two longitudinally distributed metal sheets are fixedly connected in the receiving groove, the two metal sheets have different thermal expansion coefficients, the end of the push rod away from the push block is fixedly connected to the metal sheet, the perfusion groove and the receiving groove are connected through a heat conducting plate, and the metal sheet close to the perfusion groove is in contact with the heat conducting plate.

[0011] Preferably, a positioning block in contact with the rotating shaft is fixedly connected inside the regulating box, and the connecting port of the air pipe and the regulating box is located between the positioning block and the regulating plate and closer to the positioning block.

[0012] Preferably, the perfusion assembly includes a plurality of first sandwich tanks and second sandwich tanks located on the support frame, the second sandwich tank is located above the first sandwich tank, the lower end of the first sandwich tank is connected to a three-way valve, one end of the three-way valve is connected to a first hose, the free end of the first hose is connected to the lower end of the temperature control tube, the first hose is connected to a branch pipe, the free end of the branch pipe extends into the temperature control tube and is connected to one end of the guide groove, and the liquid in the branch pipe flows unidirectionally to the first hose.

[0013] Preferably, the other end of the three-way valve is connected to a second hose, the free end of the second hose is connected to the corresponding bottom of the second sandwich tank, a plurality of peristaltic pumps are arranged on one side of the support frame, the first hose is clamped on the corresponding peristaltic pump, and an isolated heart is suspended at the lower end of the temperature control tube.

[0014] Preferably, the constant temperature component includes a water bath, which is arranged on one side of the support frame, and the lower side of the first interlayer tank closest to the water bath is connected to the inlet of the water bath through a water inlet pipe, and a disc tube is fixedly connected inside the temperature control tube. The liquid in the first interlayer tank closest to the water bath flows through the adjacent disc tubes and the first interlayer tank in sequence through the first flow path pipe according to the principle of bottom-in and top-out.

[0015] Preferably, the upper end of the disc tube farthest from the water bath is connected to the corresponding lower end of the second interlayer tank via a second flow path pipe, two adjacent second interlayer tanks are connected via a third flow path pipe, and the upper end of the second interlayer tank closest to the water bath is connected to the outlet of the water bath via a water outlet pipe.

[0016] Preferably, the volume of the first interlayer tank is smaller than that of the second interlayer tank, and the diameters of the first flow tube, the second flow tube and the third flow tube are all larger than the diameters of the first hose and the second hose.

[0017] Technical effects and advantages of the present invention: 1. The present invention can not only simultaneously or independently perform perfusion experiments on an isolated heart with multiple drugs or under different conditions, thereby improving experimental efficiency and data comparability, but also allow perfusion experiments on multiple isolated hearts to be performed simultaneously, thereby improving experimental efficiency and flexibility, and supports free switching of multiple perfusion fluids to meet various experimental needs. At the same time, the constant temperature component can keep the temperature of the perfusion fluid constant, ensuring that the perfusion fluid can maintain normal in vitro physiological activities of the isolated heart. The setting of the peristaltic pump can accurately control the outflow rate of the perfusion fluid to meet the simulation of cardiac output of different model animals, thereby ensuring the accuracy of the experimental results and further improving the efficiency and diversity of isolated heart experiments.

[0018] 2. The present invention senses the temperature of the perfusion fluid through the heat conduction plate and the metal sheet, so that when the temperature does not reach a constant temperature, the perfusion fluid can only flow from the guide groove through the branch pipe and flow to the first hose again under the action of the peristaltic pump, so that the perfusion fluid circulates, and at the same time, a large amount of perfusion fluid is prevented from accumulating in the temperature control tube, and the problem of uncontrollable perfusion rate is avoided. When the temperature of the perfusion fluid reaches a constant temperature, under the action of the metal sheet with different thermal expansion coefficients, the metal sheet drives the two baffles to swing to another state through the adjustment component, so that the perfusion fluid flows from the guide groove to the bottom of the temperature control tube and flows into the isolated heart for perfusion experiment. When the temperature of the perfusion fluid is too high, the metal sheet can drive the two baffles to change their states again through the adjustment component, so that the perfusion fluid flows through the branch pipe to the first hose for circulation. In this way, it can not only ensure that the perfusion fluid flows to the isolated heart at a constant temperature, but also ensure that the perfusion fluid can maintain the normal physiological activities of the isolated heart, while ensuring the accuracy of the experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a structural schematic diagram of the thermostatic component in the present invention; Figure 3 It is a partial structural schematic diagram of the perfusion assembly in the present invention; Figure 4 It is a schematic cross-sectional view of the structure of the second sandwich tank in the present invention; Figure 5 It is a structural schematic diagram of the temperature control tube in the present invention; Figure 6 It is a schematic cross-sectional view of the structure of the temperature control tube in the present invention; Figure 7 For the present invention Figure 6 A partial enlarged view of the middle A; Figure 8 It is an overall schematic diagram of the guide column in the present invention; Fig. 9 It is a schematic cross-sectional view of the structure of the guide column in the present invention; Fig.10 for Fig. 9 A partial enlarged view of point B in the middle; Fig.11 It is a structural schematic diagram of the temperature control flow guiding component in the present invention; Fig.12 It is a schematic diagram of the structure of the regulating component in the present invention.

[0020] In the figure: 1, support frame; 2, perfusion assembly; 201, temperature control tube; 202, first interlayer tank; 203, second interlayer tank; 204, three-way valve; 205, first hose; 206, branch pipe; 207, second hose; 208, peristaltic pump; 209, isolated heart; 3. Temperature control guide assembly; 301. Guide column; 302. Guide groove; 303. Irrigation groove; 304. Rotating shaft; 305. Baffle; 4. Adjustment assembly; 401. Receiving groove; 402. Piston cylinder; 403. Piston plate; 404. Air pipe; 405. Adjustment box; 406. Adjustment plate; 407. Push block; 408. Guide groove; 409. Push rod; 410. Insert rod; 411. Metal sheet; 412. Heat conduction plate; 413. Positioning block; 5. Constant temperature assembly; 501. Water bath; 502. Water inlet pipe; 503. Coil pipe; 504. First flow path pipe; 505. Second flow path pipe; 506. Third flow path pipe; 507. Water outlet pipe. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Embodiment 1

[0022] Currently, conventional perfusion systems usually have only a single channel and can only perfuse a single isolated heart. This is inefficient and difficult to meet the experimental requirements of simultaneously studying the effects of multiple drugs or different conditions on the isolated heart. Therefore, this embodiment is specially invented to solve the above problems.

[0023] like Figures 1 to 6 As shown, a multi-channel myocardial in vitro perfusion system of this embodiment includes a support frame 1, and a perfusion assembly 2 is arranged on the support frame 1 for perfusing the isolated myocardium; the perfusion assembly 2 also includes a plurality of temperature control tubes 201 that are distributed at intervals in the transverse direction and fixedly connected to the support frame 1. The perfusion assembly 2 includes a plurality of first interlayer tanks 202 and second interlayer tanks 203 located on the support frame 1, the second interlayer tank 203 is located above the first interlayer tank 202, the lower end of the first interlayer tank 202 is connected to a three-way valve 204, one end of the three-way valve 204 is connected to a first hose 205, the free end of the first hose 205 is connected to the lower side of the temperature control tube 201, the other end of the three-way valve 204 is connected to a second hose 207, the free end of the second hose 207 is connected to the bottom of the corresponding second interlayer tank 203, a plurality of peristaltic pumps 208 are arranged on one side of the support frame 1, the first hose 205 is clamped on the corresponding peristaltic pump 208, and an isolated heart 209 is suspended at the lower end of the temperature control tube 201.

[0024] The thermostatic assembly 5 is arranged on the outside of the perfusion assembly 2, and is used to maintain the temperature of the fluid in the perfusion assembly 2. The thermostatic assembly 5 includes a water bath 501, which is arranged on one side of the support frame 1. The lower side of the first interlayer tank 202 closest to the water bath 501 is connected to the inlet of the water bath 501 through the water inlet pipe 502, and a disc tube 503 is fixedly connected in the temperature control tube 201. The liquid in the first interlayer tank 202 closest to the water bath 501 flows through the adjacent disc tubes 503 and the first interlayer tank 202 in sequence according to the principle of bottom-in and top-out through the first flow path pipe 504. The upper end of the disc tube 503 farthest from the water bath 501 is connected to the lower end of the corresponding second interlayer tank 203 through the second flow path pipe 505, and the two adjacent second interlayer tanks 203 are connected through the third flow path pipe 506. Finally, the upper end of the second interlayer tank 203 close to the water bath 501 is connected to the water outlet of the water bath 501 through the water outlet pipe 507.

[0025] The volume of the first interlayer tank 202 is smaller than that of the second interlayer tank 203 , and the diameters of the first flow tube 504 , the second flow tube 505 and the third flow tube 506 are all larger than the diameters of the first hose 205 and the second hose 207 .

[0026] In actual use, the liquid in the water bath 501 is first heated to a constant temperature, and then injected into the first interlayer tank 202 through the inlet of the water bath 501 and the water inlet pipe 502, and according to the principle of bottom-in and top-out, the heated liquid flows into the path of the disc tube 503, the first interlayer tank 202, the disc tube 503, the first interlayer tank 202 and the disc tube 503 in the direction away from the water bath 501 through the first flow path pipe 504, and the disc tube 503 farthest from the water bath 501 injects the liquid into the second interlayer tank 203 through the second flow path pipe 505, and the liquid in the second interlayer tank 203 flows through The third flow path pipe 506 flows into the adjacent second interlayer tank 203, and the liquid in the second interlayer tank 203 closest to the water bath 501 flows back into the water bath 501 through the outlet pipe 507, thereby forming an insulation circuit. Since the first interlayer tank 202 and the second interlayer tank 203 both include an interlayer space and a tank body, and since the insulation liquid flows in the interlayer space of the first interlayer tank 202 and the second interlayer tank 203 and follows the principle of bottom-in and top-out, the insulation liquid can better heat and insulate the perfusion fluid in the tank body, thereby ensuring that the temperature of the perfusion fluid is constant and reducing the influence of external factors on the experimental results.

[0027] When conducting a perfusion experiment, the isolated heart 209 is first hung and fixed on the lower end of the temperature control tube 201, and the temperature control tube 201 is inserted into the artery of the isolated heart 209, and then different drugs are injected into the first sandwich tank 202 and the second sandwich tank 203. When different drugs are injected into the isolated heart 209 at the same time for a perfusion experiment, the peristaltic pump 208 is first started, and the perfusion flow rate is adjusted so that the peristaltic pump 208 absorbs the drugs in the first sandwich tank 202 and the second sandwich tank 203 under the action of negative pressure through the first hose 205, the three-way valve 204 and the second hose 207, and the perfusion The fluid is injected into the temperature control tube 201 through the first hose 205. Since the disc tube 503 is located in the temperature control tube 201, the perfusion fluid in the temperature control tube 201 can be better heated and kept warm, ensuring that the perfusion fluid can maintain the normal physiological activities of the isolated heart 209 in the outside world and reduce the impact of external factors on the experimental results. After the perfusion fluid in the temperature control tube 201 is injected into the isolated heart 209, the second hose 207 can be connected to different second interlayer tanks 203 to achieve perfusion experiments on multiple isolated hearts 209 under different conditions or with different drugs at the same time, and at this time, the experimental data under different conditions can be recorded.

[0028] In summary, through the configuration of the perfusion component 2 and the constant temperature component 5, not only can the isolated heart 209 be subjected to perfusion experiments with multiple drugs or under different conditions simultaneously or independently, thereby improving the experimental efficiency and data comparability, but also multiple isolated hearts 209 can be subjected to perfusion experiments simultaneously, thereby improving the experimental efficiency and flexibility, and supporting the free switching of multiple perfusion fluids to meet various experimental requirements. At the same time, the constant temperature component 5 can maintain the temperature of the perfusion fluid constant to ensure that the perfusion fluid can maintain the normal in vitro physiological activities of the isolated heart 209. The configuration of the peristaltic pump 208 can accurately control the outflow rate of the perfusion fluid to meet the requirements of simulating the cardiac output of different model animals, thereby ensuring the accuracy of the experimental results and further improving the efficiency and diversity of the experiments on the isolated heart 209. Embodiment 2

[0029] In the above process, it is found that when the perfusion experiment is performed, since the liquid in the constant temperature component 5 forms a constant temperature circuit, and there is unexhausted gas in the first hose 205 and the second hose 207, it is not conducive to heating and keeping the perfusion liquid warm. At the same time, the heating and insulation of the perfusion liquid are not in place, resulting in the perfusion liquid not reaching a constant temperature and flowing into the isolated heart 209, resulting in a large temperature error, which is not only not conducive to the normal physiological activities of the isolated heart 209 in the outside world, but also easily affects the experimental results. Therefore, further improvements are made based on the above embodiments.

[0030] like Figures 6 to 12As shown, the temperature control guide component 3 is arranged in the temperature control tube 201, and the temperature control guide component 3 also includes a guide column 301 fixedly connected to the temperature control tube 201, and a transversely extending and through-going guide groove 302 is provided in the guide column 301, and a perfusion groove 303 connected to the guide groove 302 is provided on the guide column 301, and baffles 305 are hinged at both ends of the guide groove 302 through a rotating shaft 304. When one of the baffles 305 blocks the guide groove 302, the other baffle 305 no longer blocks the guide groove 302.

[0031] The adjusting component 4 is arranged inside the guide column 301, and is used for adjusting the blocking state of the baffle 305. The adjusting component 4 includes a receiving groove 401 opened on the guide column 301, a piston cylinder 402 with closed two ends is fixedly connected in the receiving groove 401, a piston plate 403 is slidably connected in the piston cylinder 402, both ends of the piston cylinder 402 are connected to an air pipe 404, and both ends of the receiving groove 401 are fixedly connected to an adjusting box 405, the free end of the air pipe 404 is connected to the corresponding adjusting box 405, the end of the rotating shaft 304 close to the receiving groove 401 passes through the corresponding adjusting box 405, and the rotating shaft 304 is fixedly connected to an adjusting plate 406 that is slidably connected to the inside of the adjusting box 405.

[0032] A push block 407 is fixedly connected to the piston plate 403, and one end of the push block 407 away from the piston plate 403 extends out of the piston cylinder 402 and is slidably connected to the piston cylinder 402. A guide groove 408 is provided on the end surface of the push block 407 away from the piston plate 403, and a push rod 409 is slidably connected in the accommodating groove 401. The free end of the push rod 409 is fixedly connected to an insertion rod 410, and the insertion rod 410 is inserted into the guide groove 408. When the push rod 409 slides, the push block 407 slides back and forth along the axial direction of the piston cylinder 402 under the action of the guide groove 408.

[0033] Two longitudinally distributed metal sheets 411 are fixedly connected in the receiving groove 401. The thermal expansion coefficients of the two metal sheets 411 are different, and the thermal expansion coefficient of the metal sheet 411 close to the top rod 409 is small. The end of the top rod 409 away from the push block 407 is fixedly connected to the metal sheet 411. The perfusion groove 303 and the receiving groove 401 are connected through the heat conducting plate 412, and the metal sheet 411 close to the perfusion groove 303 is in contact with the heat conducting plate 412.

[0034] A positioning block 413 in contact with the rotating shaft 304 is fixedly connected inside the regulating box 405 . The connection port between the air pipe 404 and the regulating box 405 is located between the positioning block 413 and the regulating plate 406 , and is closer to the positioning block 413 .

[0035] The first hose 205 is connected to a branch pipe 206 , the free end of which extends into the temperature control tube 201 and communicates with one end of the guide groove 302 . A one-way valve is provided in the branch pipe 206 , and the liquid in the branch pipe 206 flows unidirectionally toward the first hose 205 .

[0036] In actual use, when the perfusion fluid that has not been heated to a constant temperature flows into the temperature control tube 201, the perfusion groove 303 and the guide groove 302 formed on the guide column 301 form an inverted T shape, so that one end of the guide groove 302 is connected to the inside of the temperature control tube 201, and the other end of the guide groove 302 is connected to the first hose 205 through the branch pipe 206, and one baffle 305 blocks the guide groove 302 flowing to the temperature control tube 201, and the other baffle 305 does not block the guide groove 302 flowing to the branch pipe 206. In this state, when the temperature of the perfusion fluid flowing into the perfusion trough 303 does not reach a constant temperature, the perfusion fluid flows into the branch pipe 206 through the perfusion trough 303 and the guide trough 302. Since a one-way valve is provided in the branch pipe 206, the perfusion fluid can only flow toward the first hose 205. At this time, the perfusion fluid will not flow into the isolated heart 209 through the temperature control tube 201, and the perfusion fluid will flow into the first hose 205 again through the branch pipe 206, thereby preventing too much perfusion fluid from being retained in the temperature control tube 201.

[0037] When the refluxed perfusion fluid is heated again through the first hose 205 and flows into the temperature control tube 201, the temperature of the perfusion fluid flowing into the perfusion tank 303 reaches a constant temperature. The temperature of the perfusion fluid is transferred to the metal sheet 411 in the receiving tank 401 through the heat conducting plate 412. Since the thermal expansion coefficients of the two metal sheets 411 are different, the metal sheet 411 is bent toward the push rod 409 due to thermal expansion, so that the deformation of the metal sheet 411 drives the push rod 409 to slide, and the push rod 409 slides toward the direction close to the piston cylinder 402. Since the shape of the guide groove 408 is an isosceles trapezoid, it should be noted that due to the large angle of the inclined groove of the guide groove 408, the push rod 409 slides a small distance, which can make the push block 407 slide a large distance (and can also be moved by the lever). When the push rod 409 drives the insertion rod 410 to slide from the inclined groove of the guide groove 408 to the vertical groove, the push rod 409 drives the push block 407 to slide in the direction of the branch pipe 206 through the insertion rod 410 and the guide groove 408, and the push block 407 drives the piston plate 403 to slide, so that the piston plate 403 pushes the gas in the piston cylinder 402 to squeeze into the regulating box 405 through the air pipe 404, and the connecting port of the air pipe 404 and the regulating box 405 is located between the positioning block 413 and the regulating plate 406, and the gas squeezed into the positioning block 413 and the regulating plate 406 drives the regulating plate 406 to rotate along the regulating box 405, so that the regulating plate 406 drives a baffle 305 to swing through the rotating shaft 304, and gradually blocks the guide groove 302 flowing to the branch pipe 206.

[0038] When a baffle 305 gradually blocks the guide groove 302 flowing toward the branch pipe 206, the angle of the baffle 305 changes and it can be affected by the gravity of the perfusion fluid, so that the swing of the baffle 305 can be accelerated, and the guide groove 302 flowing toward the branch pipe 206 can be quickly blocked. At the same time, the piston plate 403 sucks the gas in the regulating box 405 away from the branch pipe 206 into the piston cylinder 402 through the trachea 404, so that the gas pressure between the side of the regulating plate 406 close to the trachea 404 and the positioning block 413 is reduced, so that the gas pressure on the side of the regulating plate 406 away from the trachea 404 is strong. Under the action, the adjustment plate 406 rotates along the adjustment box 405 toward the trachea 404, and the adjustment plate 406 drives the other baffle 305 to swing toward the perfusion groove 303 through the rotating shaft 304, so that the other baffle 305 no longer blocks the guide groove 302 flowing to the temperature control tube 201. At this time, the perfusion fluid that reaches a constant temperature flows to the bottom of the temperature control tube 201 through the perfusion groove 303 and the guide groove 302, and flows into the isolated heart 209, so that the perfusion fluid maintains the normal physiological activities of the isolated heart 209 in the outside world, thereby conducting experiments on the isolated heart 209 and ensuring the accuracy of the experimental data.

[0039] When the temperature of the perfusion fluid is too high, the heat is transferred to the metal sheet 411 through the heat conducting plate 412. Since the thermal expansion coefficient of the metal sheet 411 close to the heat conducting plate 412 is greater than the thermal expansion coefficient of the metal sheet 411 far from the heat conducting plate 412, the metal sheet 411 expands and deforms again to drive the push rod 409 to slide in the direction close to the piston cylinder 402. Since the shape of the guide groove 408 is an isosceles trapezoid, when the push rod 409 drives the insertion rod 410 to slide from the vertical groove of the guide groove 408 to the inclined groove, the push rod 409 drives the push block 407 to slide in the direction away from the branch pipe 206 through the insertion rod 410 and the guide groove 408, and the push block 407 drives the piston plate 403 to slide, so that the piston plate 403 pushes the gas in the piston cylinder 402 to squeeze into the direction away from the branch pipe 206 through the air pipe 404. 6, the gas squeezed into the positioning block 413 and the adjusting plate 406 drives the adjusting plate 406 to rotate along the adjusting box 405, so that the adjusting plate 406 drives the baffle 305 away from the branch pipe 206 to swing and block the guide groove 302 again through the rotating shaft 304, and at the same time, the piston plate 403 absorbs the gas in the regulating box 405 through the air pipe 404, so that the baffle 305 close to the branch pipe 206 no longer blocks the guide groove 302, and the perfusion fluid is prompted to flow from the guide groove 302 to the branch pipe 206 and the first hose 205 to circulate, so as to avoid the perfusion fluid with too high temperature from being injected into the isolated heart 209, thereby ensuring the accuracy of the experimental data. When the perfusion is no longer performed, the metal sheet 411 dissipates heat and recovers. At this time, the above movement is repeated in the direction to restore the two baffles 305 to the initial state.

[0040] In summary, the perfusion liquid temperature is sensed by the heat conducting plate 412 and the metal sheet 411, so that when the temperature does not reach a constant temperature, the perfusion liquid can only flow from the guide groove 302 through the branch pipe 206 and flow to the first hose 205 again under the action of the peristaltic pump 208, so that the perfusion liquid circulates, and at the same time, a large amount of perfusion liquid is prevented from accumulating in the temperature control tube 201, and the problem of uncontrollable perfusion rate is avoided. When the perfusion liquid temperature reaches a constant temperature, under the action of the metal sheet 411 with different thermal expansion coefficients, the metal sheet 411 drives the two hoses 205 through the adjustment component 4. The baffles 305 swing to another state, so that the perfusion fluid flows from the guide groove 302 into the bottom of the temperature control tube 201 and into the isolated heart 209 for perfusion experiment. When the temperature of the perfusion fluid is too high, the metal sheet 411 can drive the two baffles 305 to change their states again through the adjustment component 4, so that the perfusion fluid flows through the branch pipe 206 to the first hose 205 for circulation. In this way, it can not only ensure that the perfusion fluid flows to the isolated heart 209 at a constant temperature, but also ensure that the perfusion fluid can maintain the normal physiological activities of the isolated heart 209, while ensuring the accuracy of the experimental data.

[0041] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A multi-channel myocardial in vitro perfusion system, comprising a support frame (1), characterized in that: Also includes: A perfusion assembly (2), the perfusion assembly (2) being arranged on the support frame (1) and used for perfusing isolated myocardium, the perfusion assembly (2) further comprising a plurality of temperature control tubes (201) which are arranged at intervals in the transverse direction and fixedly connected to the support frame (1); A temperature control flow guide component (3), the temperature control flow guide component (3) being arranged in the temperature control tube (201), the temperature control flow guide component (3) further comprising a flow guide column (301) fixedly connected to the temperature control tube (201), the flow guide column (301) being provided with a flow guide groove (302) extending transversely and penetrating therein, the flow guide column (301) being provided with a perfusion groove (303) communicating with the flow guide groove (302), baffles (305) being hingedly connected at both ends of the flow guide groove (302) via rotating shafts (304), and when one of the baffles (305) blocks the flow guide groove (302), the other baffle (305) no longer blocks the flow guide groove (302); an adjusting component (4), the adjusting component (4) being arranged inside the guide column (301) and being used for adjusting the blocking state of the baffle (305); A constant temperature component (5), the constant temperature component (5) being arranged outside the perfusion component (2) and being used for maintaining the temperature of the fluid in the perfusion component (2).

2. The myocardial in vitro perfusion system according to claim 1, characterized in that: The regulating assembly (4) comprises a receiving groove (401) provided on the guide column (301), a piston cylinder (402) with closed ends fixedly connected in the receiving groove (401), a piston plate (403) slidably connected in the piston cylinder (402), air pipes (404) connected at both ends of the piston cylinder (402), regulating boxes (405) fixedly connected at both ends of the receiving groove (401), a free end of the air pipe (404) communicating with a corresponding regulating box (405), an end of the rotating shaft (304) close to the receiving groove (401) passing through the corresponding regulating box (405), and an regulating plate (406) slidably connected to the inside of the regulating box (405) fixedly connected to the rotating shaft (304).

3. The myocardial in vitro perfusion system according to claim 2, characterized in that: A push block (407) is fixedly connected to the piston plate (403). One end of the push block (407) away from the piston plate (403) extends out of the piston cylinder (402) and is slidably connected to the piston cylinder (402). A guide groove (408) is provided on one end surface of the push block (407) away from the piston plate (403). A push rod (409) is slidably connected in the accommodating groove (401). A plug rod (410) is fixedly connected to the free end of the push rod (409). The plug rod (410) is inserted into the guide groove (408). When the push rod (409) slides, the push block (407) slides back and forth along the axial direction of the piston cylinder (402) under the action of the guide groove (408).

4. The myocardial ex vivo perfusion system according to claim 3, characterized in that: Two longitudinally distributed metal sheets (411) are fixedly connected in the receiving groove (401), and the two metal sheets (411) have different thermal expansion coefficients. The end of the push rod (409) away from the push block (407) is fixedly connected to the metal sheet (411). The perfusion groove (303) and the receiving groove (401) are connected via a heat conducting plate (412), and the metal sheet (411) close to the perfusion groove (303) is in contact with the heat conducting plate (412).

5. The myocardial ex vivo perfusion system according to claim 4, characterized in that: A positioning block (413) in contact with the rotating shaft (304) is fixedly connected inside the regulating box (405); a connection port between the air pipe (404) and the regulating box (405) is located between the positioning block (413) and the regulating plate (406), and is closer to the positioning block (413).

6. The myocardial ex vivo perfusion system according to claim 5, characterized in that: The perfusion assembly (2) comprises a plurality of first interlayer tanks (202) and second interlayer tanks (203) located on the support frame (1); the second interlayer tank (203) is located above the first interlayer tank (202); the lower end of the first interlayer tank (202) is connected to a three-way valve (204); one end of the three-way valve (204) is connected to a first hose (205); the free end of the first hose (205) is connected to the lower end of the temperature control tube (201); the first hose (205) is connected to a branch pipe (206); the free end of the branch pipe (206) extends into the temperature control tube (201) and is connected to one end of the guide groove (302); the liquid in the branch pipe (206) flows unidirectionally toward the first hose (205).

7. The myocardial ex vivo perfusion system according to claim 6, characterized in that: The other end of the three-way valve (204) is connected to a second hose (207), the free end of the second hose (207) is connected to the bottom of the corresponding second sandwich tank (203), a plurality of peristaltic pumps (208) are arranged on one side of the support frame (1), the first hose (205) is clamped on the corresponding peristaltic pump (208), and an isolated heart (209) is suspended at the lower end of the temperature control tube (201).

8. The myocardial ex vivo perfusion system according to claim 7, characterized in that: The constant temperature assembly (5) comprises a water bath (501), wherein the water bath (501) is arranged on one side of the support frame (1), and the lower side of the first sandwich tank (202) closest to the water bath (501) is connected to the inlet of the water bath (501) via a water inlet pipe (502), and a coiled tube (503) is fixedly connected inside the temperature control tube (201), and the liquid in the first sandwich tank (202) closest to the water bath (501) flows sequentially through the adjacent coiled tubes (503) and the first sandwich tank (202) through the first flow path pipe (504) according to the principle of bottom-in and top-out.

9. The isolated myocardial perfusion system according to claim 8, characterized in that: The upper end of the coiled tube (503) farthest from the water bath (501) is connected to the lower end of the corresponding second interlayer tank (203) via a second flow path pipe (505), two adjacent second interlayer tanks (203) are connected via a third flow path pipe (506), and the upper end of the second interlayer tank (203) closest to the water bath (501) is connected to the outlet of the water bath (501) via a water outlet pipe (507).

10. The myocardial ex vivo perfusion system according to claim 9, characterized in that: The volume of the first interlayer tank (202) is smaller than the volume of the second interlayer tank (203), and the diameters of the first flow tube (504), the second flow tube (505) and the third flow tube (506) are all larger than the diameters of the first hose (205) and the second hose (207).

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