Frankliniella occidentalis luring composition and application

Abstract

The invention belongs to the field of plant protection, and particularly relates to a Frankliniella occidentalis luring composition and application. The invention discloses a Frankliniella occidentalis luring composition, which is prepared from 1, 3-dimethyl benzene, p-xylene, 1, 7, 7-trimethyl tricycloheptane, 1R-alpha-pinene and 2, 6, 6-trimethyl-dicycloheptyl-2-ene, and is characterized in that the Frankliniella occidentalis luring composition is prepared from 1, 3-dimethyl benzene, p-xylene, 1, 7 The five compounds are obtained by enriching zucchini volatile matters, separating and screening the volatile matters, and the five compounds are mixed according to the volume ratio to prepare the Frankliniella occidentalis luring composition. The field test result of the Frankliniella occidentalis luring composition shows that the Frankliniella occidentalis luring composition has remarkable luring activity on Frankliniella occidentalis, is simple to prepare, environment-friendly and non-toxic to human and livestock, and has good popularization and application prospects.

Description

Technical Field

[0001] The invention belongs to the field of plant protection, and particularly relates to a western flower thrips attractant composition and application thereof. Background Art

[0002] Western flower thrips (Frankliniella occidentalis), also known as western flower thrips and alfalfa thrips, belong to the order Thysanoptera, family Thripidae, and genus Frankliniella. Western flower thrips host more than 500 species in 66 families, mainly harming vegetable crops such as peppers, eggplants, cucumbers, lettuce, and tomatoes. Western flower thrips harm the leaves, flowers, and fruits of plants. They use rasp-sucking mouthparts to absorb nutrients from plant tissues and organs to meet their own growth and development needs, and cause serious damage to crops by feeding, laying eggs, and spreading viruses. At present, the prevention and control of western flower thrips in production is still mainly chemical control. Due to the biological learning characteristics of western flower thrips, such as small individuals, easy hiding, and eggs laid inside plant tissues, prevention and control is difficult. In addition, the frequent use of chemical agents not only increases the drug resistance of western flower thrips, but also pollutes the environment. Therefore, it is very urgent to explore pollution-free, sustainable, green control technologies.

[0003] Botanical pesticides have become a hot topic in the research and development and application of new pesticides due to their safety and harmlessness to human health, environmental friendliness, and low resistance to pesticides. At present, there are a few studies on new agents for the control of western flower thrips; for example, CN112544627A discloses the use of an insecticide composition in the control of cucumber yellow-breasted thrips, rose western flower thrips, or mango tea yellow thrips. The active components of the insecticide composition include avermectin benzoate and spirotetramat. The composition has significantly enhanced activity in the control of cucumber yellow-breasted thrips, rose western flower thrips, or mango tea yellow thrips, but the composition has a poor inhibitory effect on the reproductive capacity of western flower thrips, and cannot effectively kill western flower thrips in hidden places of crops, making the pests easy to recur.

[0004] Because western flower thrips are small and well hidden, they often hide in relatively hidden living environments such as flower buds and leaf bottoms, making it difficult for conventional chemical agents to reach the hiding places of western flower thrips, resulting in the recurrence of pests. Attractants developed based on the pests' preferred food sources or their volatiles play an important role in the pests' food preference selection behavior. Attractants that have an attractant effect on insects have become an important means of green pest control. Attractants work on both female and male insects, have a wide range of effects, and can improve the control effect on pests. At present, some screening and application of thrips attractants have been reported. With the increase in the understanding of plant volatile components, compounds that have an attractant effect on thrips are still being discovered, but they are far from enough. Therefore, it is necessary to identify and screen new components that can be used for western flower thrips attractants in order to improve the trapping effect on western flower thrips. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a western flower thrips attractant composition and application. The present invention aims to collect volatiles of zucchini, further screen the zucchini volatiles to obtain substances that have an attractant effect on western flower thrips, further conduct a ratio study on the screened substances to obtain a western flower thrips attractant composition, and apply the composition to prepare a western flower thrips attractant, thereby providing a new option for the prevention and control of western flower thrips.

[0006] On the one hand, the present invention provides a western flower thrips attractant composition, which consists of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene.

[0007] Furthermore, in the western flower thrips attractant composition, the volume ratio of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclohept-2-ene is 25:20:5:15:35.

[0008] Furthermore, in the western flower thrips attractant composition, the concentration of 1,3-dimethylbenzene is 1-600 mg / L.

[0009] Furthermore, in the western flower thrips attractant composition, the concentration of p-xylene is 10-500 mg / L.

[0010] Furthermore, in the western flower thrips attractant composition, the concentration of 1,7,7-trimethyltricycloheptane is 1-300 mg / L.

[0011] Furthermore, in the western flower thrips attractant composition, the concentration of 1R-α-pinene is 10-700 mg / L.

[0012] Furthermore, in the western flower thrips attractant composition, the concentration of 2,6,6-trimethyl-bicyclohept-2-ene is 1-400 mg / L.

[0013] Furthermore, the components of the flower thrips attractant composition, 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene, are obtained by enriching zucchini volatiles, and separating and screening the enriched zucchini volatiles.

[0014] On the other hand, the present invention provides use of the western flower thrips attractant composition in preparing a western flower thrips attractant.

[0015] Furthermore, the western flower thrips attractant composition is applied to western flower thrips on cucumbers, peppers, sweet peppers, tomatoes, strawberries, grapes, orchids, and chrysanthemums.

[0016] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0017] The western flower thrips attractant composition provided by the present invention is obtained by enriching zucchini volatiles, separating and screening 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene, which have attractant activity to western flower thrips. The above 5 compounds are mixed to prepare the western flower thrips attractant composition. The field trapping test results show that the western flower thrips attractant composition provided by the present invention has significant attractant activity to western flower thrips. The western flower thrips attractant composition provided by the present invention is derived from natural plant ingredients, has significant attractant activity to western flower thrips, is green, efficient and broad-spectrum, provides a new choice for the prevention and control of thrips, and has good application prospects. DETAILED DESCRIPTION

[0018] The technical solution of the present invention is described below in conjunction with embodiments; however, the present invention is not limited to the following embodiments.

[0019] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments, but the embodiments are not intended to limit the present invention.

[0020] The experimental methods and detection methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified. The 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane described in this example is the same as the 1,7,7-trimethyltricycloheptane described in the present invention.

[0021] Example 1

[0022] This example provides a method for collecting, separating and identifying zucchini volatiles.

[0023] Collection of zucchini volatiles: The zucchini volatiles were collected using dynamic headspace adsorption technology. Grind the zucchini and add it to a 20 mL headspace bottle. Place it in a 60°C water bath for 5 minutes, insert the SPME needle, and extract it at 60°C for 30 minutes using the headspace method. Then, use an Agilent 7890A / 5975C gas chromatograph-mass spectrometer to analyze and inject the sample. The column oven program for the analysis and injection is 40°C, hold for 5 minutes, and then 8°C min. -1 The temperature was raised to 250°C at a speed of 100 °C and kept at a constant temperature for 10 min; the running time was 41.25 min, the front inlet was high-purity He, the mode was split, the heater temperature was 250°C, the pressure was 48.75 kPa, and the total flow rate was 9 mL min -1 , split ratio 5:1; chromatographic column DB-5ms, 325℃, 30m×250μm×0.25μm; low mass number 40.0, high mass number 400.0, ion source EI source, ion source temperature 230℃, quadrupole temperature 150℃. The experiment was repeated 6 times, and all the zucchini tested were collected only once.

[0024] Separation and identification of zucchini volatiles: The collected zucchini volatiles were analyzed using an Agilent 7890A / 5975C gas chromatograph-mass spectrometer. The standard mass spectra were retrieved by a mass spectrometry computer data system based on the peaks in the total ion flow diagram to determine the information chemical component. The relative mass fraction of each chemical component was determined by the peak area normalization method to identify the chemical component structure of the zucchini volatiles.

[0025] A total of 124 zucchini volatiles were obtained through separation and identification. Through extensive literature research and the laboratory's previous accumulated research experience in attracting and killing western flower thrips, 5 compounds with higher abundance were screened from the above-isolated zucchini volatiles as alternatives for subsequent research. The 5 zucchini volatiles screened are shown in Table 1.

[0026] Table 1 Volatiles of zucchini

[0027] Serial number Element CAS Number 1 1,3-Dimethylbenzene 108-38-3 2 p-Xylene 106-42-3 3 1,7,7-Trimethyltricyclo[2.2.1.0(2,6)]heptane 508-32-7 4 1R-α-Pinene 7785-70-8 5 2,6,6-Trimethyl-bicyclo[3.1.1]hept-2-ene 80-56-8

[0028] Example 2

[0029] The zucchini volatiles obtained in Example 1 were prepared with liquid paraffin into preparations with concentrations of 0.01wt%, 0.1wt% and 1wt%, respectively, and behavioral regulation tests were performed on western flower thrips one by one to preliminarily screen out compounds that have an attractant effect on the behavior of western flower thrips.

[0030] Test insects: Western flower thrips were an experimental population raised for multiple generations in an artificial climate chamber in the inventor's laboratory using fresh green bean pods. The rearing conditions were a photoperiod of 14L:10D, a relative humidity of 65%±5%, and a temperature of (26±1)°C. Western flower thrips that were 3 days old were selected for testing.

[0031] Test method: The test was conducted using a Y-shaped olfactometer, which was made of transparent glass, with a base length of 16 cm, two arms of 15 cm, an inner diameter of 2 cm, and an angle of 60° between the two arms. When conducting behavioral tests on adult western flower thrips, one arm of the "Y"-shaped olfactometer was connected to a odor source bottle containing volatile substances of zucchini, and the other arm was connected to a odor source bottle containing liquid paraffin as a control. The air was ventilated for 5 minutes to fill the pipe with odor. The air flow rate of the two arms of the "Y"-shaped tube was controlled at 200 mL / min, the indoor temperature was controlled at (25±1)℃, and the relative humidity was 60% to 80%. Before the test, the adult western flower thrips were starved for 5 hours, and each test insect was tested only once. Introduce a test insect into the base arm end of the glass tube of the "Y" type olfactometer and test for 5 minutes. When the test insect crawls over 1 / 3 of a certain measuring tube arm of the "Y" type olfactometer and can maintain it for more than 30 seconds, it is considered that the insect has chosen the tube arm, indicating that the compound has an attractant effect on western flower thrips; otherwise, it is recorded as no choice. Five test insects were tested for each treatment, and repeated 3 times. After each treatment test, the "Y" type olfactometer and other test supplies were rinsed with anhydrous ethanol, and then the glass accessories were placed in an electric constant temperature drying blower at 180℃ for 120 minutes, and the plastic and rubber accessories were placed in an electric constant temperature drying blower at 80℃ for 60 minutes. Liquid paraffin was used as a control.

[0032] The results of the behavioral test of western flower thrips are shown in Table 2. The mass spectra of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene showed that the five compounds had an attractant effect on western flower thrips.

[0033] Table 2 Results of behavioral test on western flower thrips

[0034]

[0035] Example 3

[0036] This example is a behavioral test experiment on 5 different concentrations of zucchini volatiles on western flower thrips.

[0037] The 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene used in this example were purchased from Beijing Bailingwei Technology Co., Ltd., with a purity of ≥95%;

[0038] Liquid paraffin was used as solvent to prepare gradient solutions of the above five volatiles:

[0039] 1,3-Dimethylbenzene: (0.1, 1, 10, 100, 200, 400, 600, 800, 1000 mg / L);

[0040] p-Xylene: (1, 10, 100, 200, 300, 400, 500, 600, 700 mg / L);

[0041] 1,7,7-Trimethyltricyclo[2.2.1.0(2,6)]heptane: (0.1, 1, 10, 100, 200, 300, 400, 500 mg / L);

[0042] 1R-α-pinene: (1, 10, 100, 200, 400, 500, 600, 700, 800, 900 mg / L);

[0043] 2,6,6-Trimethyl-bicyclo[3.1.1]hept-2-ene: (0.1, 1, 10, 100, 200, 300, 400, 500, 600 mg / L).

[0044] Determination method: The test was conducted using a Y-shaped olfactometer, which was made of transparent glass, with a base length of 16 cm, two arms of 15 cm, an inner diameter of 2 cm, and an angle of 60° between the two arms. When conducting behavioral tests on adult western flower thrips, one arm of the "Y"-shaped olfactometer was connected to a odor source bottle containing volatile substances of zucchini, and the other arm was connected to a odor source bottle containing liquid paraffin as a control. The air was ventilated for 5 minutes to fill the pipe with odor. The air flow rate of the two arms of the "Y"-shaped tube was controlled at 200 mL / min, the indoor temperature was controlled at (25±1)℃, and the relative humidity was 60% to 80%. Before the test, the adult western flower thrips were starved for 5 hours, and each test insect was tested only once. Introduce a test insect into the base arm end of the glass tube of the "Y" type olfactometer and test for 5 minutes. When the test insect crawls over 1 / 3 of a certain measuring tube arm of the "Y" type olfactometer and can maintain it for more than 30 seconds, it is considered that the insect has chosen the tube arm, indicating that the compound has an attractant effect on western flower thrips; otherwise, it is recorded as no choice. Five test insects were tested for each treatment, and repeated 3 times. After each treatment test, the "Y" type olfactometer and other test supplies were rinsed with anhydrous ethanol, and then the glass accessories were placed in an electric constant temperature drying blower at 180℃ for 120 minutes, and the plastic and rubber accessories were placed in an electric constant temperature drying blower at 80℃ for 60 minutes. Liquid paraffin was used as a control.

[0045] The results of the Y-type olfactometer are shown in Table 3. The results showed that 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene had significant attractant activity to the behavior of western flower thrips, among which 1,3-dimethylbenzene had significant attractant activity to the behavior of western flower thrips when the concentration range was 1-600 mg / L, p-xylene had significant attractant activity to the behavior of western flower thrips when the concentration range was 10-500 mg / L, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane had significant attractant activity to the behavior of western flower thrips when the concentration range was 1-300 mg / L, 1R-α-pinene had significant attractant activity to the behavior of western flower thrips when the concentration range was 1-700 mg / L, and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene had significant attractant activity to the behavior of western flower thrips when the concentration range was 1-400 mg / L.

[0046] Table 3 Behavioral test of 5 different concentrations of zucchini volatiles on western flower thrips

[0047]

[0048]

[0049] The test results are shown in Table 3. The number of insects trapped in each treatment was less than or equal to 3, which was considered as having no attraction to western flower thrips. The concentration of 1,3-dimethylbenzene in the range of 1-600 mg / L attracted western flower thrips; the concentration of p-xylene in the range of 10-500 mg / L attracted western flower thrips; the concentration of 1,7,7-trimethyltricycloheptane in the range of 1-300 mg / L attracted western flower thrips; the concentration of 1R-α-pinene in the range of 10-700 mg / L attracted western flower thrips; and the concentration of 2,6,6-trimethyl-bicyclohept-2-ene in the range of 1-400 mg / L attracted western flower thrips.

[0050] Example 4

[0051] This example provides the preparation of a western flower thrips attractant composition.

[0052] 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene, which have significant attractant activity on the behavior of western flower thrips in Example 3, were prepared into different western flower thrips attractant compositions according to the volume ratio of 1,3-dimethylbenzene: p-xylene: 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane: 1R-α-pinene: 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene = 25:20:5:15:35.

[0053] #1 Western flower thrips attractant composition: 10 mg / L 1,3-dimethylbenzene, 10 mg / L p-xylene, 1 mg / L 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 10 mg / L 1R-α-pinene and 1 mg / L 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #1 Western flower thrips attractant composition for use.

[0054] #2 Western flower thrips attractant composition: 100 mg / L of 1,3-dimethylbenzene, 50 mg / L of p-xylene, 10 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 50 mg / L of 1R-α-pinene and 5 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #2 Western flower thrips attractant composition for standby use.

[0055] #3 Western flower thrips attractant composition: 600 mg / L of 1,3-dimethylbenzene, 100 mg / L of p-xylene, 100 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 100 mg / L of 1R-α-pinene and 10 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #3 Western flower thrips attractant composition for standby use.

[0056] #4 Western flower thrips attractant composition: 100 mg / L of 1,3-dimethylbenzene, 10 mg / L of p-xylene, 200 mg / L 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 200 mg / L of 1R-α-pinene and 100 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #4 Western flower thrips attractant composition for standby use.

[0057] #5 Western flower thrips attractant composition: 200 mg / L of 1,3-dimethylbenzene, 200 mg / L of p-xylene, 50 mg / L 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 400 mg / L of 1R-α-pinene and 200 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare a #5 Western flower thrips attractant composition for use.

[0058] #6 Western flower thrips attractant composition: 500 mg / L of 1,3-dimethylbenzene, 500 mg / L of p-xylene, 300 mg / L of 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 500 mg / L of 1R-α-pinene and 300 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #6 Western flower thrips attractant composition for use.

[0059] #7 Western flower thrips attractant composition: 600 mg / L of 1,3-dimethylbenzene, 100 mg / L of p-xylene, 300 mg / L 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 700 mg / L of 1R-α-pinene and 400 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare #7 Western flower thrips attractant composition for standby use.

[0060] #8 Western flower thrips attractant composition: 600 mg / L of 1,3-dimethylbenzene, 300 mg / L of p-xylene, 100 mg / L 1,7,7-trimethyltricyclo[2.2.1.0(2,6)]heptane, 400 mg / L of 1R-α-pinene and 200 mg / L of 2,6,6-trimethyl-bicyclo[3.1.1]hept-2-ene are prepared in a volume ratio of 25:20:5:15:35 to prepare a #8 Western flower thrips attractant composition for use.

[0061] Example 5

[0062] This example is a field trapping test of western flower thrips.

[0063] Use a pipette to take 200 μL of the western flower thrips attractant composition and add it dropwise onto the septum of the rubber stopper cleaned with n-hexane. After the n-hexane evaporates, add 1 ml of dichloromethane in portions. After the dichloromethane evaporates, the western flower thrips attractant composition bait cores are obtained. Each bait core contains the western flower thrips attractant composition. The prepared bait cores are sealed in aluminum foil and stored at -20°C.

[0064] When conducting trapping tests in the field, the trap cores of the western flower thrips attractant composition were fixed on the sticky insect board. CK was a treatment without the trap cores of the western flower thrips attractant composition; groups #1 to #8 were treatments with the trap cores of the western flower thrips attractant composition. Three replicates were set for each treatment, randomly arranged, and the interval between two adjacent sticky insect boards was 15 to 30 meters. Starting from the second day after the setting, the number of western flower thrips trapped was recorded every morning and removed in time, and the investigation continued for 30 days.

[0065] Table 4 Field trapping test results of western flower thrips attractant composition

[0066]

[0067] The test results are shown in Table 4. All the #1-#8 western flower thrips attractant compositions have attractant activity to western flower thrips and are superior to the control group, indicating that the western flower thrips attractant composition provided by the present invention has attractant activity to western flower thrips, and the captured insect bodies are identified for sex, and most of them are female western flower thrips and a few are male western flower thrips, indicating that the western flower thrips attractant composition provided by the present invention has attractant activity to both male and female western flower thrips, among which #6, #7, and #8 western flower thrips attractant compositions have higher attractant activity to western flower thrips.

[0068] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A western flower thrips attractant composition, characterized in that: The western flower thrips attractant composition consists of 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloalkane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene.

2. The western flower thrips attractant composition according to claim 1, characterized in that: In terms of volume ratio, the ratio of 1,3-dimethylbenzene:p-xylene:1,7,7-trimethyltricycloheptane:1R-α-pinene:2,6,6-trimethyl-bicyclohept-2-ene is 25:20:5:15:

35.

3. The western flower thrips attractant composition according to claim 2, characterized in that: The concentration of the 1,3-dimethylbenzene is 1-600 mg / L.

4. The western flower thrips attractant composition according to claim 2, characterized in that: The concentration of p-xylene is 10-500 mg / L.

5. The western flower thrips attractant composition according to claim 2, characterized in that: The concentration of the 1,7,7-trimethyltricycloheptane is 1-300 mg / L.

6. The western flower thrips attractant composition according to claim 2, characterized in that: The concentration of the 1R-α-pinene is 10-700 mg / L.

7. The western flower thrips attractant composition according to claim 2, characterized in that: The concentration of the 2,6,6-trimethyl-bicyclohept-2-ene is 1-400 mg / L.

8. The western flower thrips attractant composition according to claim 1, characterized in that: The components of the flower thrips attractant composition, 1,3-dimethylbenzene, p-xylene, 1,7,7-trimethyltricycloheptane, 1R-α-pinene and 2,6,6-trimethyl-bicyclohept-2-ene, are obtained by enriching and separating zucchini volatiles.

9. Use of the western flower thrips attractant composition according to any one of claims 1 to 8 in the preparation of a western flower thrips attractant.

10. The use according to claim 9, characterized in that: The western flower thrips are the western flower thrips on cucumbers, peppers, sweet peppers, tomatoes, strawberries, grapes, orchids, and chrysanthemums.

Images