Pesticide composition and application

Abstract

The invention belongs to the technical field of pesticide control, and relates to a pesticide composition and application. The pesticide composition comprises an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is a neonicotinoid compound, and the mass ratio of the active component A to the active component B is 1: 85-45: 1. The pesticide composition disclosed by the invention has the advantages of obvious synergistic effect, fast pesticide effect, long lasting period, increased acting sites, expanded prevention and control spectrum and capability of reducing the field dosage of effective components, so that the production and use components are reduced, and the generation of drug resistance of pests and pest mites is delayed.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide control, and specifically relates to a pesticide composition and application thereof. Background Art

[0002] Neonicotinoids are highly effective, broad-spectrum, and have good root systemic, contact, and stomach toxicity. They can be used for stem and leaf treatment, soil treatment, and seed treatment, making them a relatively effective class of insecticides for controlling piercing-sucking mouthparts pests, small lepidoptera, and coleoptera pests. With the widespread use of neonicotinoids, the frequency is increasing, and multiple targets have developed varying degrees of resistance to neonicotinoids.

[0003] In the process of agricultural production, the control of various types of harmful insects mainly relies on the application of a large number of pesticide products. The large-scale, frequent and unreasonable use of pesticides has led to the continuous enhancement of the resistance of harmful insects, making prevention and control more difficult and causing more serious damage to crops. By mixing the compound of formula (I) with a neonicotinoid compound agent, the insecticide and acaricide effect has a synergistic effect under a certain mass ratio, and can treat different pests and mites, expand the prevention and control spectrum, improve the prevention effect of the agent, and reduce the generation of pest resistance. At present, no relevant reports on the mixing of the compound (I) with neonicotinoid pesticides for the prevention and control of pests and mites have been found. Summary of the invention

[0004] The purpose of the present invention is to provide a pesticide composition containing neonicotinoid compounds which has synergistic effects, reduces resistance and has low use cost.

[0005] To achieve the above object, the technical solution adopted by the present invention is: a pesticide composition, wherein the active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) The active ingredient B is a neonicotinoid compound;

[0006] Further, the neonicotinoid compound includes flubendiamide, flupyradifur, clothianidin, thiamethoxam, acetamiprid, thiacloprid, imidacloprid, dinotefuran, nitenpyram, nicotine, flupyradone, trifluanid, nithiazine or chlorthiazide;

[0007] Furthermore, the neonicotinoid compound is thiamethoxam, acetamiprid, imidacloprid, and nitenpyram;

[0008] Furthermore, the mass ratio between the active ingredient A and the active ingredient B is 1:85 to 45:1 or any value between the above values;

[0009] Furthermore, the mass ratio between the active ingredient A and the active ingredient B is 1:50 to 40:1;

[0010] Furthermore, the neonicotinoid compound is thiamethoxam, and the mass ratio of the compound of formula (I) to thiamethoxam is 1:33 to 40:1.

[0011] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:33, 1:18, 1:16, 1:12, 1:6, 2:1, 4:1, 10:1, 16:1, 20:1, 35:1, 40:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:16 to 20:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:16, 1:12, 1:6, 2:1, 4:1, 10:1, 16:1, 20:1;

[0014] Furthermore, the neonicotinoid compound is acetamiprid, and the mass ratio of the compound of formula (I) to acetamiprid is 1:30 to 37:1.

[0015] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30, 1:25, 1:20, 1:17, 1:15, 1:8, 2:15, 1:3, 3:1, 4:1, 8:1, 15:1, 20:1, 37:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30 to 20:1,

[0017] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30, 1:25, 1:20, 1:17, 1:15, 1:8, 2:15, 1:3, 3:1, 4:1, 8:1, 15:1, 20:1;

[0018] Furthermore, the neonicotinoid compound is imidacloprid, and the mass ratio of the compound of formula (I) to imidacloprid is 1:50 to 30:1.

[0019] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50, 1:25, 1:20, 1:10, 1:7, 1:3, 1:1, 3:1, 7:1, 10:1, 17:1, 20:1, 30:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50 to 20:1.

[0021] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50, 1:25, 1:20, 1:10, 1:7, 1:3, 1:1, 3:1, 7:1, 10:1, 17:1, 20:1;

[0022] Furthermore, the neonicotinoid compound is nitenpyram, and the mass ratio of the compound of formula (I) to nitenpyram is 1:50 to 30:1;

[0023] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:50, 1:26, 1:13, 1:5, 1:2, 5:1, 10:1, 13:1, 20:1, 25:1, 30:1;

[0024] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:26 to 25:1;

[0025] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:26, 1:13, 1:5, 1:2, 5:1, 10:1, 13:1, 20:1, 25:1;

[0026] Furthermore, based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%;

[0027] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1% to 85%;

[0028] Furthermore, the pesticide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0029] Furthermore, the pesticide composition further comprises an adjuvant, and the adjuvant is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0030] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0031] Further, the dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0032] Further, the emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0033] Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0034] Furthermore, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0035] Furthermore, the antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0036] Furthermore, the defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C 8 -C 10 One or more of fatty alcohols; and / or

[0037] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0038] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0039] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0040] Furthermore, the synergist is selected from synergist phosphine and piperonyl ether; and / or

[0041] Furthermore, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0042] Furthermore, the pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0043] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0044] Further, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0045] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0046] Application of the pesticide composition of the present invention in preventing and controlling agricultural, forestry and sanitary pests and mites;

[0047] Furthermore, the agricultural and forestry health pests and mites are pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0048] Further, the pests include aphids and whiteflies;

[0049] Furthermore, the harmful mites include Panonychus citri, Tetranychus cinnabarinus, Tetranychus urticae, and Tetranychus truncatus;

[0050] The present invention also provides a method for using the neonicotinoid-containing pesticide composition as described above, specifically applying an effective dose to the pests to be controlled or the medium in which they grow.

[0051] The pesticide composition of the present invention has the following advantages:

[0052] 1) The pesticide composition of the present invention has obvious synergistic effect, fast efficacy, long lasting effect, more action sites, and a wide control spectrum;

[0053] 2) The pesticide composition of the present invention has a significant effect and can reduce the amount of active ingredients used in the field, thereby reducing production and use components, which is beneficial to delaying the development of pesticide resistance in pests and mites.

[0054] 3) The pesticide composition of the present invention is environmentally friendly and has low toxicity to humans and animals. DETAILED DESCRIPTION

[0055] The present invention is further described below with reference to the examples. The percentages in the examples are all percentages by weight, but the present invention is not limited thereto.

[0056] The composition of the present invention can be provided in the form of a preparation. It can be prepared into a suspension, water dispersible granules, aqueous emulsions, granules, wettable powders, dispersible oil suspensions, etc. as needed. The content of the active ingredient in the composition of the present invention depends on the application amount when used alone, and also depends on the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies according to the type of preparation of the composition.

[0057] Preparation Example

[0058] Embodiment 1:

[0059] 42% formula (Ⅰ) compound·thiamethoxam wettable powder (6:36)

[0060] Formula: 6% compound of formula (I), 36% thiamethoxam, 3% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8% tea saponin, 2% sodium lauryl sulfate, 2% citric acid, kaolin makes up the balance;

[0061] Preparation method: Active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and crushed and mixed evenly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0062] Embodiment 2:

[0063] 40% formula (Ⅰ) compound acetamiprid wettable powder (4:36)

[0064] Formula: 4% compound of formula (I), 36% acetamiprid, 3% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8.5% tea saponin, 2.5% sodium lauryl sulfate, and kaolin to make up the balance;

[0065] Preparation method: Same as Example 1.

[0066] Embodiment 3:

[0067] 42% formula (Ⅰ) compound·nitenpyram wettable powder (7:35)

[0068] Formula: 7% compound of formula (I), 35% nitenpyram, 5% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8% tea saponin, 2% sodium lauryl sulfate, and kaolin to make up the balance;

[0069] Preparation method: Same as Example 1.

[0070] Embodiment 4:

[0071] 27% formula (Ⅰ) compound·imidacloprid suspension (3:24)

[0072] Formula: 3% compound of formula (I), 24% imidacloprid, 1.5% sulfonate formaldehyde condensate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% styrene phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 1% sodium sorbate, 4.5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0073] Preparation method: Add wetting dispersant, defoamer, etc. to water and stir evenly, then add active ingredients, use zirconium oxide beads, and use a sand mill to wet grind to D 90 (90% of the particle size) <5 μm, and a crushed slurry is obtained. A thickener, an antifreeze agent, and a preservative are added to the crushed slurry and mixed evenly, and deionized water is added to 100%, and a suspension product is obtained by high-speed shearing.

[0074] Embodiment 5:

[0075] 64% formula (Ⅰ) compound·thiamethoxam water dispersible granules (8:56)

[0076] Formula: 8% compound of formula (I), 56% thiamethoxam, 6% sodium salt of polycarboxylate, 2% dispersant NNO, 6% naphthalenesulfonate formaldehyde condensate, 2.5% polycarboxylate anion (WG5), 2% sodium dodecyl sulfate, 2% citric acid, 4% sodium sulfate, and kaolin makes up the balance;

[0077] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, appropriate water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and sieving.

[0078] Embodiment 6:

[0079] 56% Formula (Ⅰ) compound · Acetamiprid water dispersible granules (7:49)

[0080] Formula: 7% compound of formula (I), 49% acetamiprid, 2% sodium lignin sulfonate, 3.5% sodium polycarboxylate, 8% naphthalene sulfonate formaldehyde condensate, 2.5% polycarboxylate anion (WG5), 2% sodium dodecyl sulfate, 4.5% white carbon black, and kaolin makes up the balance;

[0081] Preparation method: Same as Example 5.

[0082] Embodiment 7:

[0083] 48% formula (Ⅰ) compound·imidacloprid water dispersible granules (6:42)

[0084] Formula: 6% compound of formula (I), 42% imidacloprid, 6% sodium polycarboxylate, 7.5% naphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 2.5% polycarboxylate anion (WG5), 4% starch, and kaolin to make up the balance;

[0085] Preparation method: Same as Example 5.

[0086] Embodiment 8:

[0087] 60% compound of formula (I)·nitenpyram water dispersible granules (10:50)

[0088] Formula: 10% compound of formula (I), 50% nitenpyram, 6% sodium salt of polycarboxylate, 8% naphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 2.5% polycarboxylate anion (WG5), 4% sodium sulfate, and kaolin makes up the balance;

[0089] Preparation method: Same as Example 5.

[0090] Indoor biological activity assay

[0091] Examples refer to the Guidelines for Indoor Bioassay Tests for Pesticides Part 6: Insect Dipping Method NY / T 1154.6-2006; Part 7: Determination of Combined Effects of Mixtures NY / T 1154.7-2006; Part 12: Slide Dipping Method for Spider Mites NY / T 1154.12-2008.

[0092] Specific indoor toxicity test:

[0093] 1) Test target: Panonychus citri

[0094] Test method: spider mite slide dipping method;

[0095] Test insect source: Panonychus citri; select nymphs raised indoors and in the same physiological state. Cut double-sided tape into 2 cm long pieces and stick them on one end of a glass slide. Then select healthy mites and stick their backs on the double-sided tape. Put 30 mites per slide in a container padded with a wet sponge, cover it, and place it at (25±1)℃. After 2 hours, examine under a microscope, remove dead and injured individuals, and make up 25 mites per slide.

[0096] Preparation of reagent: The test reagent was prepared into a mother solution using an organic solvent (acetone), and then 5 series of mass concentrations were prepared using a 0.1% Tween 80 aqueous solution in an equal proportion.

[0097] Experimental treatment: Immerse the glass slide in the drug solution and gently shake it for 5 seconds, then take it out and absorb the excess drug solution with absorbent paper. Place it on a white porcelain plate lined with a wet sponge, cover it with a light-transmitting plastic film, and place it at (25±1)℃ for observation.

[0098] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0099] Investigation time: Check the death of test insects 24 hours after treatment, record the total number of mites and the number of dead mites, and calculate the mortality rate.

[0100] 2) Test target: aphids

[0101] Test method: insect immersion method;

[0102] Test insects: healthy, 2nd-instar aphids with consistent growth and development;

[0103] Drug preparation: The test drug was dissolved in acetone and prepared into 5 series of concentrations using 0.1% Tween 80 aqueous solution.

[0104] Experimental treatment: Select 2nd instar larvae raised indoors and in the same physiological state, and immerse the test insects in the drug solution for 5 seconds using an insect immersion device. Treat 15 larvae each time, and repeat 4 times. The control group is treated with 0.1% Tween 80 aqueous solution. After treatment, the insects are transferred to a culture dish with a diameter of 9.0 cm covered with filter paper for breeding, and fresh leaves are replaced every day.

[0105] The treated test insects were placed in an artificial intelligence culture room at 25±1°C, a light duration of L:D=14h:10h, and a relative humidity of 65%±5% for breeding.

[0106] Investigation method: After the treatment with the pesticide, the mortality of the test insects was investigated 24 hours later. The criterion for judging the death of the test insects was that the insect body was obviously shrunken or could not crawl normally after being pricked with a needle. The total number of insects and the number of dead insects were recorded separately.

[0107] Data statistics and analysis:

[0108] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula, with the results retained to two decimal places:

[0109]

[0110] Where:

[0111] P——mortality rate, in percentage (%);

[0112] K——indicates the number of dead insects, in units of heads;

[0113] N——represents the total number of insects treated, in heads.

[0114]

[0115] Where:

[0116] P 1 ——Adjusted mortality rate, expressed as percentage (%);

[0117] P t ——Treatment mortality rate, expressed as percentage (%);

[0118] P 0 ——Blank control mortality rate, in percentage (%).

[0119] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0120] The data can be processed by probability value analysis. The DPS statistical analysis system can be used to calculate the toxicity regression line, LC 50 The activity of the test agent on the biological test material was evaluated by using the value, 95% confidence limit and correlation coefficient r.

[0121] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0122]

[0123] Where:

[0124] ATI——Actual Toxicity Index of Mixture;

[0125] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0126] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0127] TTI=TI A*P A +TI B *P B

[0128] Where:

[0129] TTI – Theoretical Toxicity Index of Mixtures;

[0130] TI A ——A: toxicity index of the agent;

[0131] P A ——The percentage of agent A in the mixture, in percentage (%);

[0132] TI B ——Toxicity index of agent B;

[0133] P B ——The percentage of agent B in the mixture, in percentage (%).

[0134]

[0135] Where:

[0136] CTC – Co-toxicity coefficient;

[0137] ATI——Actual Toxicity Index of Mixture;

[0138] TTI - Theoretical Toxicity Index of Mixture.

[0139] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0140] Results of the combined toxicity test of Panonychus citri:

[0141] LC of the compound of formula (I) against Panonychus citri 50 The concentration of the compound of formula (I) is 3.156 mg / L. The mixture of the compound of formula (I) and thiamethoxam, acetamiprid, imidacloprid and nitenpyram has a good synergistic effect on Panonychus citri.

[0142] As shown in Table 1, when the mass ratio of the compound of formula (I) to thiamethoxam is 1:63-35:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:33-35:1, the co-toxicity coefficient is greater than 120, which shows a good synergistic effect on citrus mites.

[0143] Table 1 The results of toxicity test of different ratios of the compound of formula (I) and thiamethoxam to Panonychus citri

[0144]

[0145] As shown in Table 2, when the mass ratio of the compound of formula (I) to acetamiprid is 1:30-40:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:30-15:1, the co-toxicity coefficient is greater than 120, which shows a good synergistic effect on citrus mites.

[0146] Table 2 Toxicity test results of different ratios of the compound of formula (I) and acetamiprid to Panonychus citri

[0147]

[0148] As shown in Table 3, when the mass ratio of the compound of formula (I) to imidacloprid is 1:85-30:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:50-30:1, the co-toxicity coefficient is greater than 120, which shows a good synergistic effect on citrus mites.

[0149] Table 3 Toxicity test results of different ratios of the compound of formula (I) and imidacloprid to Panonychus citri

[0150]

[0151] As shown in Table 4, when the mass ratio of the compound of formula (I) to nitenpyram is 1:50-45:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:50-20:1, the co-toxicity coefficient is greater than 120, which shows a good synergistic effect on citrus mites.

[0152] Table 4 Toxicity test results of different ratios of the compound of formula (I) and nitenpyram to Panonychus citri

[0153]

[0154] Results of the combined toxicity test of the mixed pair against aphids:

[0155] LC of the compound of formula (I) against aphids 50 The LC values ​​of thiamethoxam, acetamiprid, imidacloprid and nitenpyram against aphids are 127.517 mg / L. 50 The concentrations of these compounds were 5.293 mg / L, 2.338 mg / L, 24.715 mg / L and 3.935 mg / L respectively. The compound of formula (I) mixed with thiamethoxam, acetamiprid, imidacloprid and nitenpyram had a good combined effect on aphids.

[0156] As shown in Table 5, when the mass ratio of the compound of formula (I) to thiamethoxam is 1:18 to 40:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on the control effect of aphids.

[0157] Table 5 Toxicity test results of different ratios of the compound of formula (I) and thiamethoxam to aphids

[0158]

[0159] As shown in Table 6, when the mass ratio of the compound of formula (I) to acetamiprid is 1:25-37:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on the control of aphids. Among them, when the mass ratio of the compound of formula (I) to acetamiprid is 1:8-20:1, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect.

[0160] Table 6 Toxicity test results of different ratios of the compound of formula (I) and acetamiprid to aphids

[0161]

[0162] As shown in Table 7, when the mass ratio of the compound of formula (I) to imidacloprid is 1:25-17:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on the control of aphids. Among them, when the mass ratio of the compound of formula (I) to imidacloprid is 1:25-7:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect.

[0163] Table 7 Toxicity test results of different ratios of the compound of formula (I) and imidacloprid to aphids

[0164]

[0165] As shown in Table 8, when the mass ratio of the compound of formula (I) to nitenpyram is 1:26-30:1, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect on the control of aphids.

[0166] Table 8 Toxicity test results of different ratios of the compound of formula (I) and nitenpyram to aphids

[0167]

[0168]

[0169] Field efficacy examples

[0170] Test crops and targets: citrus navel orange, citrus spider mites, citrus aphids;

[0171] Experimental location: citrus orchard in Changyang County, Yichang City;

[0172] Experimental design: Each group of experiments had 6 citrus trees (fixed plant marking), 3 replicates, and random arrangement.

[0173] Test time: The test application time for controlling citrus red spider mites is May 2, 2023, and the test application time for controlling citrus aphids is August 29, 2023.

[0174] Spraying equipment: 3WBS-16 backpack electric sprayer, first spray the water control area, then spray the test agent area. The weather was clear on the day of spraying, and the whole tree was sprayed evenly, and the front and back of the leaves were sprayed until water drops fell. The amount of liquid used for each tree was 1.8L.

[0175] Experimental environment: The cultivation conditions of all experimental plots were uniform and consistent, and the same fertilizer and water management level was adopted. The citrus fruits grew well, and no agricultural operations such as fertilization, irrigation and drainage were carried out during the experiment.

[0176] Test drug:

[0177] Table 9 Field test pesticides and dosage

[0178]

[0179] Survey method: Before each spraying, and 1d and 7d after spraying, we conducted tree and leaf surveys. We selected 3 leaves from each citrus tree in the east, south, west, and north directions, marked them with labels, counted the number of live insects on the front and back sides, calculated the control effect (prevention efficiency), and conducted a significant difference analysis.

[0180] Calculation formula and data analysis:

[0181]

[0182]

[0183] Field efficacy test results:

[0184] The test results show that the control effects of different pesticides on citrus red spider mites are shown in Table 10. It can be seen from the table that 1 day after the application of the pesticide, 64% of the compound of formula (I)·thiamethoxam water dispersible granules (8:56), 56% of the compound of formula (I)·acetamiprid water dispersible granules (7:49), 48% of the compound of formula (I)·imidacloprid water dispersible granules (6:42), and 60% of the compound of formula (I)·nitram water dispersible granules (10:50) showed good rapid effectiveness, with a control effect of more than 80%; On day 7, the protective effects of 64% compound (I)·thiamethoxam water dispersible granules (8:56), 56% compound (I)·acetamiprid water dispersible granules (7:49), 48% compound (I)·imidacloprid water dispersible granules (6:42), and 60% compound (I)·nitram water dispersible granules (10:50) were improved to 91.86%, 91.64%, 90.81%, and 91.79%, respectively. Each mixed preparation also showed good persistence, with a protective effect of more than 90%.

[0185] 1d and 7d after application, there was no significant difference in the control effect of 64% compound of formula (I)·thiamethoxam water dispersible granules (8:56), 56% compound of formula (I)·acetamiprid water dispersible granules (7:49), 48% compound of formula (I)·imidacloprid water dispersible granules (6:42), and 60% compound of formula (I)·nitram water dispersible granules (10:50) on citrus red spider mites, but there was a significant difference between them and the other treatments, which was significantly higher than the control single agent and mixed preparation (20% imidacloprid·triazotin wettable powder).

[0186] Table 10 The control effect of different pesticides on citrus red spider mites

[0187]

[0188]

[0189] Note: The above data are the average of 3 repetitions, with two decimal places, and the difference in protective efficacy is at the 0.05% level.

[0190] The test results show that the control effects of different pesticides on aphids are shown in Table 11. It can be seen from the table that the insect population base survey before application found that the insect population base of aphids in the test treatments H-1, H-2, H-3, and H-4 was less than that in other test treatments, proving that the first application of pesticides to control citrus mites can also effectively reduce the occurrence of citrus aphids in the later stage.

[0191] In addition, 1 day after the application, 64% of the compound of formula (I)·thiamethoxam water dispersible granules (8:56), 56% of the compound of formula (I)·acetamiprid water dispersible granules (7:49), 48% of the compound of formula (I)·imidacloprid water dispersible granules (6:42), and 60% of the compound of formula (I)·nitenpyram water dispersible granules (10:50) showed good rapid effectiveness, with the control effects of 83.34% and 81.58%, respectively. %, 82.97%, 84.86%; 7 days after application, the protective efficacy of 64% compound of formula (Ⅰ)·thiamethoxam water dispersible granules (8:56), 56% compound of formula (Ⅰ)·acetamiprid water dispersible granules (7:49), 48% compound of formula (Ⅰ)·imidacloprid water dispersible granules (6:42), 60% compound of formula (Ⅰ)·nitram water dispersible granules (10:50) was improved, and the protective efficacy was all above 95%.

[0192] 1d and 7d after application, there was no significant difference in the control effect of 64% compound (I)·thiamethoxam water dispersible granules (8:56), 56% compound (I)·acetamiprid water dispersible granules (7:49), 48% compound (I)·imidacloprid water dispersible granules (6:42), and 60% compound (I)·nitram water dispersible granules (10:50) on citrus aphids, but there was a significant difference between them and the other treatments, which was significantly higher than the control single agent and mixed preparation (20% imidacloprid·triazotin wettable powder).

[0193] Table 11 The control effect of different pesticides on aphids

[0194]

[0195] Note: The above data are the average of 3 repetitions, with two decimal places, and the difference in protective efficacy is at the 0.05% level.

[0196] When using pesticides to control pests in the field, try to use them during the initial period of pest occurrence so that the control effect can be best.

[0197] Safety: It was observed that the test agent treatment did not cause phytotoxicity or other adverse effects on citrus fruits compared with the blank control area.

[0198] The pesticide composition or the preparation obtained by compounding the present invention has significant prevention effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging drug retention. In addition, no compound agent was found to cause pesticide damage to crops in the test, indicating that the obtained pesticide composition or preparation can reduce production costs and use costs and is safe for crops when the insecticide synergistic synergy is improved.

[0199] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: The invention comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) The active ingredient B is a neonicotinoid compound.

2. The pesticide composition according to claim 1, characterized in that: The neonicotinoid compounds include flubendiamide, flupyradifur, clothianidin, thiamethoxam, acetamiprid, thiacloprid, imidacloprid, dinotefuran, nitenpyram, nicotine, flupyradone, trifluanid, nithiazine or chlorthiazide; Preferably, the neonicotinoid compound is thiamethoxam, acetamiprid, imidacloprid, or nitenpyram.

3. The pesticide composition according to claim 1, characterized in that: The mass ratio between the active ingredient A and the active ingredient B is 1:85 to 45:1; Preferably, the mass ratio between the active ingredient A and the active ingredient B is 1:50 to 40:

1.

4. The pesticide composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to thiamethoxam is 1:33-40:1, the mass ratio of the compound of formula (I) to acetamiprid is 1:30-37:1, the mass ratio of the compound of formula (I) to imidacloprid is 1:50-30:1, and the mass ratio of the compound of formula (I) to nitenpyram is 1:50-30:1; Preferably, the mass ratio of the compound of formula (I) to thiamethoxam is 1:16-20:1, the mass ratio of the compound of formula (I) to acetamiprid is 1:30-20:1, the mass ratio of the compound of formula (I) to imidacloprid is 1:50-20:1, and the mass ratio of the compound of formula (I) to nitenpyram is 1:26-25:

1.

5. The pesticide composition according to claim 1, characterized in that: Based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%; Preferably, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1% to 85%.

6. The pesticide composition according to claim 1, characterized in that: In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

7. The pesticide composition according to claim 6, characterized in that: The pesticide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation; Preferably, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

8. Use of the pesticide composition according to any one of claims 1 to 7 in preventing and controlling agricultural, forestry and sanitary pests and mites.

9. The use according to claim 8, characterized in that: The agricultural, forestry and sanitary pests and mites are pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton and cereal crops; Preferably, the fruit trees are citrus and apple.

10. The pesticide composition according to claim 1, characterized in that: The pesticide composition is applied to the pests to be controlled or the medium where they grow in an effective dose.

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