Application of active amino acid as herbicide

Abstract

The invention relates to the technical field of herbicides, in particular to application of active amino acid as a herbicide, and the active amino acid is selected from one or more of (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropane carboxylic acid. The four amino acid compounds have excellent barnyard grass prevention and removal activity in a certain concentration range, do not affect growth and development of rice seedlings, can be applied to development of rice field barnyard grass removal agents, and are beneficial to promoting green and organic production of rice.

Description

Technical Field

[0001] The invention relates to the technical field of herbicides, and in particular to the application of active amino acids as herbicides. Background Art

[0002] The application of chemical herbicides is the simplest, most economical and effective means for farmers to control weeds in rice fields. However, with the large-scale, single and repeated use of chemical herbicides, resistance to pesticides has emerged in rice field weeds. As one of the main malignant weeds that seriously threaten the safe production of rice worldwide, barnyard grass (Echinochloa crusgalli L.) has a large-scale and high-frequency outbreak of resistance, which greatly shortens the service life of existing barnyard grass killers. The occurrence of resistant weeds has forced farmers to increase the use of rice field herbicides, which not only increases production costs, but also seriously affects the yield and quality of crops, posing severe challenges to the ecological environment, human health and sustainable agricultural development. The problem of farmland weeds continuing to harm rice production needs to be solved urgently. Summary of the invention

[0003] The inventors have found through a large number of experimental studies that amino acid compounds with herbicidal activity can effectively inhibit the growth of barnyard grass and show good safety to rice growth. They have great potential for development as new herbicide products, and the present invention is specially proposed.

[0004] The invention provides application of active amino acids as herbicides. The active amino acids are selected from one or more of (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid.

[0005] Specifically, the application is to control weeds of the genus Echinochloa (Echinochloa Beauv.); preferably, to control barnyard grass (Echinochloa crusgalli L.); more preferably, to control barnyard grass in paddy fields.

[0006] Specifically, the application is to control the growth of barnyard grass (Echinochloa crusgalli L.) seedlings, specifically including the sprout length and / or root length of the barnyard grass seedlings.

[0007] Specifically, the present invention includes the use of (2E)-decenoyl apolipoprotein as a herbicide, and preferably the use is for controlling barnyardgrass.

[0008] Preferably, the concentration of (2E)-decenoyl apolipoprotein used in the application is 40-120 μmol / L, more preferably 80-120 μmol / L, and even more preferably 120 μmol / L.

[0009] Specifically, the present invention includes the use of L-histidine as a herbicide, preferably the use is to control barnyard grass.

[0010] Preferably, the concentration of L-histidine used in the application is 80-160 μmol / L, more preferably 80 μmol / L.

[0011] Specifically, the present invention includes the use of pipecolic acid as a herbicide, and preferably the use is to control barnyard grass.

[0012] Preferably, the concentration of pipecolic acid used in the application is 40-160 μmol / L, more preferably 40-80 μmol / L, and even more preferably 40 μmol / L.

[0013] Specifically, the present invention includes the use of 1-aminocyclopropanecarboxylic acid as a herbicide, and preferably the use is for controlling barnyard grass.

[0014] Preferably, the concentration of 1-aminocyclopropanecarboxylic acid used in the application is 40-160 μmol / L, more preferably 40-80 μmol / L, and even more preferably 40 μmol / L.

[0015] The present invention also provides a herbicide, comprising the above active amino acid as an active ingredient, and also comprising a carrier or auxiliary material that can be used in the herbicide.

[0016] Specifically, the usable carriers or excipients include one or more of antioxidants, chelating agents, surfactants, fillers, disintegrants, wetting agents, solvents and pH adjusters.

[0017] The invention finds that four amino acid compounds, namely (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid, have obvious inhibitory effects on the growth of barnyard grass seedlings under certain concentration conditions. When the concentrations of (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid are 120 μmol / L, 80 μmol / L, 40 μmol / L and 40 μmol / L respectively, the inhibition rates on the root length of barnyard grass seedlings are 23.56%, 34.98%, 50.45% and 26.15% respectively, and the inhibition rates on the sprout length of barnyard grass seedlings are 20.27%, 21.54%, 52.43% and 18.64% respectively.

[0018] The present invention also found that four amino acid compounds (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid showed good safety on the growth of rice seedlings within a certain treatment concentration range. When the (2E)-decenoyl apolipoprotein is used at a concentration of 40-80 μmol / L, it will not inhibit the root length and shoot length of allelopathic rice (PI) and non-allelopathic rice (TY) seedlings; on the contrary, when the (2E)-decenoyl apolipoprotein is at a concentration of 40 μmol / L, it can promote the shoot length growth of allelopathic rice (PI). L-histidine and pipecolic acid at all treatment concentrations (40-160 μmol / L) have no significant effect on the growth of seedlings of two tested rice varieties; when the 1-aminocyclopropanecarboxylic acid is used at a concentration of 40-120 μmol / L, it has no obvious inhibitory effect on the growth of seedlings of two tested rice varieties.

[0019] The four amino acid compounds of the invention have excellent barnyard grass control activity within a certain concentration range, do not affect the growth and development of rice seedlings, are friendly to the growth of rice seedlings, can be applied to the development of rice field barnyard grass killers, and are conducive to promoting green and organic rice production. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The above graphs show the up-regulated metabolites of rice roots in response to resistant barnyardgrass and sensitive barnyardgrass stress in Example 1.

[0021] Figure 2 It shows the WGCNA analysis of rice roots under different treatment conditions in Example 1.

[0022] Figure 3 The figure shows the effect of the candidate amino acid compounds of Example 2 on the growth of barnyard grass; the candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L.

[0023] Figure 4 The figure shows the effect of the candidate amino acid compounds in Example 3 on the growth of allelopathic rice (PI); the candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L.

[0024] Figure 5 The figure shows the effect of the candidate amino acid compounds in Example 3 on the growth of non-allelopathic rice (TY); the candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L.

[0025] Figure 6 The inhibitory effects of candidate amino acid compounds on the sprout growth and root length of barnyardgrass (A), allelopathic rice (B) and non-allelopathic rice (C) seedlings. DETAILED DESCRIPTION

[0026] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation of the present invention is described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0027] Example 1 Screening of differential amino acid compounds based on non-targeted metabolomics research

[0028] (I) Test materials

[0029] Rice Varieties:

[0030] Internationally recognized allelopathic weed-resistant rice variety 'PI312777' (abbreviated as PI) (Dilday et al., 2001). References: Robert H. Dilday, John D. Mattice, Karen A. Moldenhauer, Wengui Yan. Allelopathic potential in rice germ plasm against duck salad, red stem and barnyard grass. Journal of Crop Production, 2001, 4: 287-301.

[0031] The non-allelopathic rice variety 'Tianyou Huazhan' (abbreviated as TY) has a large promoted planting area in the Dongting Lake rice-growing area.

[0032] Echinochloa materials: (1) Materials used for companion stress treatment: Echinochloa (R) and Echinochloa (S) with the same genetic background and high resistance to quinclorac, Latin name Echinochloa crusgalli L. (2) Materials used for herbicidal activity verification: Echinochloa weeds commonly found in rice fields in Hunan Province were collected to ensure the broad spectrum of the verification results.

[0033] 2. Experimental design

[0034] The experimental design included two treatment groups: PI(R) and PI(S), indicating that allelopathic rice PI was co-cultivated with R / S barnyardgrass, respectively. The monoculture of PI without barnyardgrass stress was used as the control treatment group. Two rice seedlings of the same growth at the 1-leaf stage were transplanted into plastic pots (46 cm long × 35 cm wide × 17 cm high) filled with 15.0 kg of air-dried paddy soil. The air-dried soil was sieved through a 2 mm sieve to remove coarse materials and plant matter. The rice seedlings were watered and fertilized according to conventional field management methods. When the rice was at the 2-leaf stage, two barnyardgrass seedlings of the needle-standing stage prepared in advance were transplanted into plastic pots, and the density ratio of rice and barnyardgrass co-cultivation was 2:2. The distance between the transplanted barnyardgrass and the root system of the allelopathic rice was kept at about 5.0 cm. Other weeds in the pots were manually removed regularly to prevent additional competition. In a completely randomized design, each treatment was replicated 4 times.

[0035] Plant samples were collected when the rice had 5 to 6 leaves. Root samples of rice from the treatment group and the control group were collected, cleaned, and pretreated according to the method provided by the company. The samples were then mailed to Shanghai Meiji Biopharmaceutical Technology Co., Ltd. The company's LC-MS / MS analysis platform (Thermo Fisher, USA) was used to complete the non-targeted metabolomics determination of this experiment.

[0036] (III) Mining of differential amino acid compounds

[0037] Based on the public database of metabolite information HMDB (http: / / www.hmdb.ca / ), Metlin (https: / / metlin.scripps.edu / ) and the database built by Megi, the metabolites detected were matched and analyzed. The difference multiple FC and the variable weight value (VIP) of the orthogonal least partial squares discriminant analysis (OPLS-DA) model were combined to screen the differential metabolites. The screening criteria were: FC ≥ 2, VIP ≥ 1. The difference multiple FC of the differential metabolites = the mean of the quantitative results of the treatment group / the mean of the quantitative results of the control group.

[0038] The up-regulated metabolites in roots in response to resistant and sensitive barnyardgrass stress are shown in Figure 1 . Figure 1 The left side (PIR Root) shows the metabolites significantly upregulated in the roots of allelopathic rice under resistant barnyardgrass stress. Figure 1 The right side (PIS Root) indicates the metabolites that were significantly upregulated in the roots of allelopathic rice under sensitive barnyardgrass stress.

[0039] Depend on Figure 1It can be seen that amino acid metabolites in the roots of allelopathic rice were significantly enriched under symbiotic stress of resistant barnyardgrass; amino acid metabolites accounted for the largest proportion (23.91%) of characteristic differential metabolites. Weighted gene co-expression network analysis (WGCNA) was used to further identify the core metabolites of rice roots under barnyardgrass stress. In the WGCNA analysis, highly correlated metabolite clusters were defined as modules, and metabolites in the same module were highly correlated. A total of six co-expression modules were identified in the roots of allelopathic rice.

[0040] WGCNA analysis of rice roots under different treatment conditions is shown in Figure 2 . Figure 2 In the analysis, Yellow Module Root represents the main root metabolites in the yellow co-expression module (under resistant barnyardgrass stress), and Blue Module Root represents the main root metabolites in the blue co-expression module (under sensitive barnyardgrass stress). Among them, amino acid compounds accounted for the largest proportion (16.67%) in the yellow co-expression module, and the amino acids in the module were highly correlated. Based on the above results, the top 14 amino acid metabolites with large inter-group differences ((2E)-decenoyl apolipoprotein, sarcosine, (S)-β-aminoisobutyric acid, pipecolic acid, pipecolic acid, L-3-cyanoalanine, L-homoserine, L-glutamic acid, L-histidine, 1-aminocyclopropanecarboxylic acid, L-norleucine, 3-amino-4-methylvaleric acid, acetyl-DL-leucine, L-aspartic acid) were screened for the identification of herbicidal activity in the next step.

[0041] Example 2: Determination of the Effect of Candidate Amino Acid Compounds on the Growth of Barnyardgrass Seedlings by Petri Dish Assay

[0042] The Petri dish method was used to set a series of gradient concentrations to study the effects of different types of candidate amino acid compounds on the growth of barnyard grass seedlings. The method is as follows:

[0043] (1) Seed dormancy breaking and disinfection: The collected barnyard grass seeds were exposed to the sun outdoors for 12 hours, placed in a -20℃ refrigerator for 12 hours, and then exposed to the sun outdoors for 12 hours to break dormancy. After the seeds were cleaned, they were soaked in 3% hydrogen peroxide for 5 minutes to sterilize the seed surface, then rinsed with clean water, and then soaked in clean water at 28℃ for 12 hours. The germination rate of barnyard grass seeds after treatment was as high as over 90%.

[0044] (2) Solution preparation: The first 14 amino acid metabolites screened in Example 1 ((2E)-decenoyl apolipoprotein, sarcosine, (S)-β-aminoisobutyric acid, pipecolic acid, pipecolic acid, L-3-cyanoalanine, L-homoserine, L-glutamic acid, L-histidine, 1-aminocyclopropanecarboxylic acid, L-norleucine, 3-amino-4-methylvaleric acid, acetyl-DL-leucine, L-aspartic acid) were prepared with pure water into amino acid compound solutions with concentrations of 160, 120, 80, 40 and 0 μmol / L.

[0045] The experimental amino acid reagents are commercially available from Shanghai McLean Biochemical Technology Co., Ltd. and others.

[0046] (3) Petri dish test method: Place three layers of filter paper in a 9 cm disposable petri dish, add 3 mL of amino acid compound solution of different concentrations, and put 30 full-grained barnyard grass seeds in each petri dish. Place the petri dish in an incubator with a temperature of 28℃ / 30℃ and a light intensity of 12h / 12h. After 4 days of incubation, measure and count the root length and shoot length of the barnyard grass seedlings. Repeat the experiment three times.

[0047] (4) Test results:

[0048] The action results of candidate amino acid compounds with obvious inhibitory effects were measured and statistically analyzed.

[0049] The results of the effects of candidate amino acid compounds on the growth of barnyard grass are shown in Figure 3 ; The candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L.

[0050] Among the top 14 amino acid metabolites with the largest difference fold, four candidate compounds, (2E)-decenoyl-ACP, L-histidine, pipecolic acid, and 1-aminocyclopropanecarboxylic acid, had a significant inhibitory effect on the growth of barnyardgrass seedlings at a certain concentration.

[0051] Specific experimental data are shown in Tables 1 to 4 (different letters indicate significant differences compared with the control group, P < 0.05).

[0052] Table 1 Effects of (2E)-decenoyl-ACP on the growth of barnyardgrass seedlings

[0053] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 3.18±0.17a 0 3.82±0.195a 0 40 3.42±0.15a -7.75 3.74±0.153ab 2.09 80 3.11±0.17a 2.15 3.25±0.124bc 14.92 120 2.53±0.14a 20.27 2.92±0.122c 23.56 160 3.2±0.19a -0.82 3.61±0.127ab 5.50

[0054] As shown in Table 1, (2E)-decenoyl apolipoprotein can inhibit the sprout growth of barnyard grass seedlings at a concentration of 80-120 μmol / L; and can significantly inhibit the root growth of barnyard grass seedlings at a concentration of 80-120 μmol / L.

[0055] Table 2 Effect of L-histidine on the growth of barnyard grass seedlings

[0056] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 2.29±0.13a 0 3.23±0.166ab 0 40 2.83±0.17a -23.43 3.73±0.154a -15.48 80 1.8±0.11a 21.54 2.1±0.154c 34.98 120 2.35±0.15a -2.36 3.17±0.185ab 1.86 160 1.82±0.15a 20.70 2.98±0.131b 7.74

[0057] As shown in Table 2, L-histidine can inhibit the sprout growth of barnyard grass seedlings at a concentration of 80 μmol / L and 160 μmol / L; it can inhibit the root growth of barnyard grass seedlings at a concentration of 80-160 μmol / L. When the concentration of L-histidine is 80 μmol / L, the inhibition rate of the sprout growth and root growth of barnyard grass seedlings reaches the maximum.

[0058] Table 3 Effect of Pipecolic Acid on the Growth of Barnyardgrass Seedlings

[0059] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 3.25±0.05a 0 5.57±0.147a 0 40 1.55±0.12d 52.43 2.76±0.12c 50.45 80 1.96±0.12c 39.80 3.16±0.147c 43.27 120 2.56±0.11b 21.18 3.8±0.2b 31.78 160 2.74±0.08b 15.79 4.1±0.124b 26.39

[0060] As shown in Table 3, pipecolic acid at a concentration of 40-160 μmol / L can significantly inhibit the sprout and root growth of barnyardgrass seedlings.

[0061] Table 4 Effects of 1-aminocyclopropanecarboxylic Acid on the growth of barnyardgrass seedlings

[0062] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 2.82±0.1a 0 3.71±0.126a 0 40 2.29±0.1b 18.64 2.74±0.131b 26.15 80 2.53±0.09ab 10.37 3.24±0.15ab 12.67 120 2.79±0.08a 0.90 2.76±0.13b 25.61 160 2.38±0.1b 15.38 2.77±0.161b 25.34

[0063] As shown in Table 4, 1-aminocyclopropanecarboxylic acid can inhibit the sprout and root growth of barnyardgrass seedlings at a concentration of 40-160 μmol / L.

[0064] It can be seen from Tables 1 to 4 that, among the above four amino acids, pipecolic acid has the best control effect on barnyard grass, and can significantly inhibit the growth of the above-ground and underground parts of barnyard grass seedlings.

[0065] Example 3 Safety evaluation of four candidate amino acid compounds on rice seedlings

[0066] The effects of four candidate amino acid compounds on the growth of rice seedlings were studied by setting a series of gradient concentrations using the Petri dish method. The method is as follows:

[0067] (1) Rice seed pretreatment: After the seeds of the allelopathic rice variety 'PI312777' (PI for short) and the non-allelopathic rice variety 'Tianyou Huazhan' (TY for short) were cleaned with clean water, the surface moisture of the seeds was dried with absorbent paper, and then the seeds were soaked in 3% hydrogen peroxide for 5 minutes to disinfect the surface of the seeds, then rinsed with clean water, and then soaked in clean water at 28°C for 12 hours. The germination rate of the rice seeds after treatment was as high as over 90%.

[0068] (2) Solution preparation: The four amino acid metabolites screened in Example 2 ((2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid, and 1-aminocyclopropanecarboxylic acid) were prepared with pure water into amino acid compound solutions with concentrations of 160, 120, 80, 40, and 0 μmol / L.

[0069] The experimental amino acid reagents are commercially available from Shanghai McLean Biochemical Technology Co., Ltd. and others.

[0070] (3) Petri dish test method: Place three layers of filter paper in a 9 cm disposable Petri dish, add 3 mL of amino acid compound solution of different concentrations, and put 30 rice seeds with full grains in each Petri dish. Place the Petri dish in an incubator with a temperature of 28℃ / 30℃ and a light intensity of 12 h / 12 ​​h. After 4 days of incubation, measure and count the root length and shoot length of the rice seedlings. Repeat the experiment three times.

[0071] (4) Test results:

[0072] The results of the effects of candidate amino acid compounds on the growth of allelopathic rice (PI) are shown in Figure 4 ; The candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L. The effects of candidate amino acid compounds on the growth of non-allelopathic rice (TY) are shown in Figure 5 ; The candidate compounds from left to right are (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid; the treatment concentrations from left to right are 0, 40, 80, 120, 160 μmol / L.

[0073] Four amino acid compounds, (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid and 1-aminocyclopropanecarboxylic acid, showed good safety for rice seedling growth within a certain concentration range.

[0074] Specific experimental data are shown in Tables 5 to 12 (different letters indicate significant differences compared with the control group, P < 0.05).

[0075] Table 5 Effect of (2E)-Decenoyl-ACP on the growth of PI seedlings

[0076] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 3.19±0.14b 0 6.49±0.14a 0 40 3.73±0.16a -16.87 6.9±0.12a -6.25 80 3.63±0.11ab -13.62 6.54±0.16a -0.72 120 1.38±0.05d 56.77 4.3±0.05b 33.68 160 2.38±0.14c 25.51 4.68±0.15b 27.89

[0077] As shown in Table 5, (2E)-decenoyl apolipoprotein did not significantly inhibit the shoot and root length of PI seedlings at lower treatment concentrations (40 μmol / L and 80 μmol / L); on the contrary, at a concentration of 40 μmol / L, (2E)-decenoyl apolipoprotein had a significant promoting effect on the shoot length of PI seedlings.

[0078] Table 6 Effect of L-histidine on the growth of PI seedlings

[0079] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 4.00±0.14a 0 6.87±0.3a 0 40 4.08±0.12a -2.20 6.75±0.22a 1.82 80 4.1±0.14a -2.49 7.05±0.2a -2.5 120 3.54±0.16a 11.54 6.39±0.18a 7.10 160 3.57±0.19a 10.68 6.76±0.23a 1.68

[0080] As shown in Table 6, treatment with different concentrations of L-histidine did not significantly affect the growth of PI seedlings.

[0081] Table 7 Effect of Pipecolic Acid on the Growth of PI Seedlings

[0082] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 3.16±0.09a 0 5.28±0.24a 0 40 3.09±0.09a 2.30 5.2±0.25a 1.43 80 2.82±0.11a 10.98 5.05±0.21a 4.19 120 2.98±0.14a 5.79 5.47±0.35a -3.71 160 3.23±0.15a -1.96 5.7±0.31a -8.28

[0083] As shown in Table 7, treatment with different concentrations of pipecolic acid had no significant effect on the growth of PI seedlings.

[0084] Table 8 Effect of 1-aminocyclopropanecarboxylic Acid on the growth of PI seedlings

[0085] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 2.64±0.14ab 0 4.67±0.19a 0 40 2.31±0.28b 12.68 4.72±0.15a -0.94 80 2.69±0.30ab -1.77 4.61±0.16a 1.40 120 2.83±0.34ab -6.90 4.6±0.19a 1.67 160 2.97±0.28a -12.46 4.04±0.15a 13.49

[0086] As shown in Table 8, treatment with different concentrations of 1-aminocyclopropanecarboxylic acid had no significant effect on the growth of PI seedlings.

[0087] It can be seen from Tables 5 to 8 that, except for the higher concentration treatments of (2E)-decenoyl apolipoprotein (120 and 160 μmol / L), the above four amino acids did not show significant inhibitory effects on the growth of allelopathic rice PI seedlings.

[0088] Table 9 Effects of (2E)-Decenoyl-ACP on the growth of TY seedlings

[0089] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 4.47±0.19a 0 5.43±0.21a 0 40 3.73±0.29ab 16.44 5.25±0.40a 3.34 80 4.18±0.18ab 6.55 5.01±0.28a 7.81 120 4.08±0.19ab 8.76 4.50±0.18a 12.55 160 3.31±0.21b 25.91 4.91±0.20a 9.56

[0090] As shown in Table 9, 160 μmol / L (2E)-decenoyl apolipoprotein treatment can significantly inhibit the shoot length of TY seedlings. However, at a concentration of 40-120 μmol / L, (2E)-decenoyl apolipoprotein did not show a significant inhibitory effect on the shoot length and root length of TY seedlings.

[0091] Table 10 Effect of L-histidine on the growth of TY seedlings

[0092] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 4.47±0.19a 0 5.43±0.21a 0 40 4.35±0.19a 2.64 5.57±0.42a -2.6 80 4.25±0.25a 4.87 5.14±0.33a 5.24 120 3.91±0.15a 12.52 5.08±0.27a 6.37 160 4.06±0.27a 9.08 5.27±0.29a 2.99

[0093] As shown in Table 10, treatment with different concentrations of L-histidine did not significantly affect the growth of TY seedlings.

[0094] Table 11 Effect of Pipecolic Acid on the Growth of TY Seedlings

[0095] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 4.47±0.19a 0 5.43±0.21a 0 40 4.09±0.24a 8.43 5.39±0.30a 0.78 80 4.48±0.16a -0.24 5.24±0.30a 3.5 120 3.87±0.23a 13.38 4.95±0.33a 8.92 160 3.74±0.23a 16.24 5.40±0.32a 0.53

[0096] As shown in Table 11, treatment with different concentrations of pipecolic acid had no significant effect on the growth of TY seedlings.

[0097] Table 12 Effect of 1-aminocyclopropanecarboxylic acid on the growth of TY seedlings

[0098] Concentration (μmol / L) Bud length (cm) Inhibition rate (%) Root length (cm) Inhibition rate (%) 0 4.47±0.19a 0 5.43±0.21a 0 40 4.11±0.25a 8.09 5.2±0.45ab 4.22 80 4.07±0.28a 9.01 4.99±0.32ab 8.18 120 4.00±0.20a 10.47 4.82±0.25ab 11.27 160 4.74±0.25a -5.98 4.22±0.31b 22.27

[0099] As shown in Table 12, 160 μmol / L 1-aminocyclopropanecarboxylic acid treatment can significantly inhibit the root length of TY seedlings. However, at a concentration of 40-120 μmol / L, 1-aminocyclopropanecarboxylic acid treatment has no significant inhibitory effect on the shoot and root length of TY seedlings.

[0100] As shown in Tables 9 to 12, the higher concentration (160 μmol / L) (2E)-decenoyl apolipoprotein and 1-aminocyclopropanecarboxylic acid treatments significantly inhibited the shoot and root length of TY seedlings, respectively. However, the lower concentration (40-120 μmol / L) of the four amino acids treatments did not significantly inhibit the growth of TY seedlings.

[0101] The relevant data after significance analysis in Tables 1 to 12 are visually displayed in the form of pictures to show the inhibitory effects of candidate amino acid compounds on the sprout growth and root length of barnyard grass (A), allelopathic rice (B) and non-allelopathic rice (C). Figure 6 .in, Figure 6A is based on the data in Tables 1 to 4, B is based on the data in Tables 5 to 8, and C is based on the data in Tables 9 to 12. Different letters in the figures indicate significant differences compared with the control group (P < 0.05); Shoot indicates shoot length, and Root indicates root length.

[0102] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. The use of active amino acids as herbicides, characterized in that: The active amino acid is selected from one or more of (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid, and 1-aminocyclopropanecarboxylic acid.

2. The use according to claim 1, characterized in that: The application is to control weeds of the genus Echinochloa (Echinochloa Beauv.); preferably, to control barnyard grass (Echinochloa crusgalli L.); more preferably, to control barnyard grass in paddy fields.

3. The use according to claim 1, characterized in that: The application is to control the growth of barnyard grass seedlings, including the sprout growth and / or root growth of barnyard grass seedlings.

4. The use according to any one of claims 1 to 3, characterized in that: During the application, the concentration of (2E)-decenoyl apolipoprotein is 40-120 μmol / L, more preferably 80-120 μmol / L, and even more preferably 120 μmol / L.

5. The use according to any one of claims 1 to 3, characterized in that: The concentration of L-histidine used in the application is 80-160 μmol / L, more preferably 80 μmol / L.

6. The use according to any one of claims 1 to 3, characterized in that: The concentration of pipecolic acid used in the application is 40-160 μmol / L, more preferably 40-80 μmol / L, and even more preferably 40 μmol / L.

7. The use according to any one of claims 1 to 3, characterized in that: The concentration of 1-aminocyclopropanecarboxylic acid used in the application is 40-160 μmol / L, more preferably 40-80 μmol / L, and more preferably 40 μmol / L.

8. A herbicide, characterized in that: The active amino acid is used as an active ingredient, and also includes a carrier or auxiliary material that can be used in the herbicide; the active amino acid is selected from one or more of (2E)-decenoyl apolipoprotein, L-histidine, pipecolic acid, and 1-aminocyclopropanecarboxylic acid.

Images