Preparation method and application of algae extract

Abstract

The invention provides a preparation method and application of an algae extract, and the preparation method comprises the following steps: degreasing algae: extracting the algae by using an organic solvent to obtain degreased algae tissues; performing water extraction on the degreased algae tissue to obtain an algae aqueous solution; and concentrating the algae aqueous solution to obtain an algae extract, wherein the algae are algae with complete cell walls. According to the present invention, the simple preparation method and the application of the algae extract can be provided, and the prepared algae extract is rich in nucleic acid, carbohydrates, amino acids, inorganic salts and other beneficial substances, has good anti-wrinkle, anti-aging, soothing, moisturizing, fine wrinkle fading and other effects, and can be used as the functional component of cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine industry, and in particular to a method for preparing an algae extract and an application thereof. Background Art

[0002] Microalgae, as a nutrient-rich biological resource, has received extensive attention. Microalgae contain a large amount of mineral nutrients such as calcium, iron, magnesium, zinc, copper, selenium, multiple vitamins, phytosterols, canthaxanthin, zeaxanthin, and phenolic compounds.

[0003] Algae extracts are substances extracted from microalgae by various means. Many algae extracts have many applications in medicine, food, cosmetics, health products, etc. For example, algae extracts are usually used as emollients and moisturizers in cosmetics. The existing algae extraction method usually involves first breaking the algae cells, then extracting them with petroleum ether and ethanol for multiple times, and finally extracting them with water reflux to obtain algae extracts.

[0004] However, there is still a lack of reports on the specific components of algae extracts, and the existing microalgae extraction methods are relatively complicated to operate, with low efficiency and high cost for large-scale preparation. Summary of the invention

[0005] The present disclosure is made in view of the above-mentioned prior art conditions, and its purpose is to provide a simple preparation method of algae extract and application thereof.

[0006] To this end, the first aspect of the present disclosure provides a method for preparing an algae extract, comprising the following steps: defatting the algae: extracting the algae using an organic solvent, the extraction temperature being 60°C to 90°C, the time being 1h to 3h, to obtain defatted algae tissue; water extracting the defatted algae tissue: boiling the defatted algae tissue in water, the boiling temperature being 80°C to 100°C, the time being 1h to 5h, to obtain an algae aqueous solution; and concentrating the algae aqueous solution to obtain an algae extract; wherein the algae is algae with intact cell walls, and the algae extract comprises 15% to 50% nucleic acids, 10% to 30% carbohydrates, 10% to 30% amino acids, and 5% to 30% inorganic salts.

[0007] In the first aspect of the present disclosure, the algae is first defatted, which can separate the high value-added polyunsaturated fatty acids in the algae and improve the utilization rate of the raw materials, and can also reduce the inconvenience caused by oil in the subsequent processing process; secondly, the defatted algae tissue is subjected to water extraction, which can dissolve various water-soluble substances in the defatted algae tissue into water, so as to obtain microalgae extracts rich in nucleic acids, carbohydrates, amino acids and inorganic salts; finally, the algae aqueous solution is concentrated to help obtain a product dosage form that is easy to store and use; among them, the use of algae with intact cell walls for defatting can help simplify the process and improve the extraction efficiency on the one hand, and also help to remove the fat in the algae as much as possible on the other hand; the preparation method disclosed in the present disclosure has a simple process, does not need to use a variety of different organic solvents to extract the algae repeatedly, and can be applied to large-scale industrial production. Thus, a simple method for preparing an algae extract can be provided, and the obtained microalgae extract is rich in nucleic acids, carbohydrates, amino acids and inorganic salts, etc., and can be used in fields such as food and cosmetics.

[0008] In the preparation method involved in the first aspect of the present disclosure, optionally, the organic solvent includes any one or more combinations of ethanol, n-hexane, petroleum ether and n-heptane, and the mass ratio of the organic solvent to the algae is 2: 1 to 10: 1. This can help to efficiently defatting the algae.

[0009] In the preparation method of the first aspect of the present disclosure, optionally, the defatted algae tissue is dried to make the defatted algae tissue in a dry powder form before water extraction. In this case, the defatted algae tissue can be separated from the organic solvent containing lipids as much as possible, reducing the inconvenience caused by oil in the subsequent processing process.

[0010] In the preparation method involved in the first aspect of the present disclosure, optionally, when the defatted algae tissue is subjected to water extraction, the mass volume ratio of the defatted algae tissue to water is 5 g:1 L to 20 g:1 L. This can help to efficiently extract the defatted algae tissue.

[0011] In the preparation method involved in the first aspect of the present disclosure, optionally, the method of concentrating the algae aqueous solution is vacuum concentration, and the temperature of the vacuum concentration is 70° C. to 100° C. This can help improve the concentration efficiency.

[0012] In the preparation method involved in the first aspect of the present disclosure, optionally, the method further includes drying the obtained product after concentrating the algae aqueous solution to obtain the algae extract in powder form. Thus, the algae extract can be applied to scenarios where powdered substances are required, and the algae extract powder is easier to store and use than the unconcentrated algae aqueous solution.

[0013] In the preparation method of the first aspect of the present disclosure, optionally, the algae is Nannochloropsis spp. In this case, Nannochloropsis spp. contains abundant active ingredients, so that the obtained algae extract can be rich in active ingredients.

[0014] A second aspect of the present disclosure provides an algae extract, which is prepared by any one of the preparation methods described in the first aspect of the present disclosure.

[0015] The third aspect of the present disclosure provides a cosmetic comprising an algae extract, wherein the algae extract comprises 15% to 50% nucleic acid, 10% to 30% saccharide, 10% to 30% amino acid, and 5% to 30% inorganic salt. In the present disclosure, it is verified that the algae extract has good anti-wrinkle, anti-aging, soothing, moisturizing and fine line fading effects, and using it as a functional component of cosmetics can obtain cosmetics with good anti-wrinkle, anti-aging, soothing, moisturizing and fine line fading effects.

[0016] In the cosmetics involved in the third aspect of the present disclosure, optionally, in the cosmetics, the concentration of the algae extract is 0.01%-0.5%, thereby being able to help obtain cosmetics with good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects.

[0017] In the cosmetics involved in the third aspect of the present disclosure, optionally, the conductivity of the solution of the algae extract at a concentration of 0.3%-0.5% is 600 μS / cm-1500 μS / cm.

[0018] In the cosmetics involved in the third aspect of the present disclosure, optionally, the elastase inhibition rate of the solution of the algae extract at a concentration of 0.1%-0.3% is not less than 14%; the upregulation rate of the algae extract solution at a concentration of 2g / L-5g / L in promoting type I collagen secretion is not less than 165%; the hyaluronidase inhibition rate of the algae extract solution at a concentration of 0.2%-0.3% is not less than 40%; the 8h moisturizing rate of the algae extract solution at a concentration of 0.05%-0.3% is not less than 55%. Thus, it can help to provide a cosmetic with good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects.

[0019] In the cosmetics involved in the third aspect of the present disclosure, optionally, the carbohydrate substance includes any one or more of (1→3,1→4)-β glucan, anionic sulfated heterorhamnan, (1→3,1→4)-α mannan, and ribose, and among the carbohydrate substances, the degree of sulfation of low molecular weight polysaccharides with a molecular weight below 10 KDa is 5%-15%, and the degree of sulfation of high molecular weight polysaccharides with a molecular weight above 10 KDa is 40%-50%.

[0020] According to the present disclosure, a preparation method of an algae extract and its application can be provided. The preparation method is simple and can be applicable to large-scale industrial production. The prepared algae extract is rich in beneficial substances such as nucleic acids, sugars, amino acids and inorganic salts, and has good anti-wrinkle, anti-aging, soothing, moisturizing and fine line fading effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a flow chart showing a preparation method involved in an example of the present disclosure.

[0022] Figure 2 It is a typical anti-aging diagram involved in the examples of the present disclosure.

[0023] Figure 3 is a graph showing the moisture retention rates of the samples according to the examples of the present disclosure.

[0024] Figure 4 is a schematic diagram showing the periorbital area of ​​a subject before administration of an algae extract in an embodiment of the present disclosure.

[0025] Figure 5 is a schematic diagram showing the periorbital area of ​​a subject after administration of an algae extract in accordance with an embodiment of the present disclosure. DETAILED DESCRIPTION

[0026] Hereinafter, the preferred embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. In the following description, the same symbols are assigned to the same components, and repeated descriptions are omitted. In addition, the accompanying drawings are only schematic diagrams, and the ratio of the dimensions of the components or the shapes of the components may be different from the actual ones.

[0027] It should be noted that the terms "including" and "having" and any variations thereof in the present disclosure, such as a process, method, system, product or device that includes or has a series of steps or units, are not necessarily limited to those steps or units clearly listed, but may include or have other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0028] The first aspect of the present disclosure relates to a method for preparing an algae extract, which is a method for extracting a target substance from algae. In the present disclosure, the method for preparing an algae extract may be referred to as a preparation method, or may be referred to as an extraction method or a method for extracting a microalgae extract.

[0029] The second aspect of the present disclosure relates to an algae extract, which is prepared by the preparation method of the first aspect of the present disclosure.

[0030] The third aspect of the present disclosure relates to a cosmetic comprising an algae extract. In the present disclosure, it is verified that the algae extract has good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects, and can be used as a functional component of cosmetics.

[0031] The fourth aspect of the present disclosure relates to the use of an algae extract in inhibiting elastase. In particular, in the present disclosure, it is verified that the algae extract has a significant inhibitory effect on elastase, and has good effects such as anti-wrinkle and lightening fine lines.

[0032] Hereinafter, the preparation method of the algae extract and the algae extract involved in the present disclosure will be described in conjunction with the accompanying drawings.

[0033] Figure 1 is a flow chart showing a preparation method involved in an example of the present disclosure.

[0034] In some examples, the preparation method may include: defatting the algae to obtain defatted algae tissue (step S10); extracting the defatted algae tissue with water to obtain an algae aqueous solution (step S20); and concentrating the algae aqueous solution to obtain an algae extract (step S30). In this case, first defatting the algae can, on the one hand, separate the high value-added polyunsaturated fatty acids in the algae to improve the utilization rate of the raw materials, and on the other hand, reduce the inconvenience caused by oil in the subsequent processing process; secondly, extracting the defatted algae tissue with water can dissolve various water-soluble substances in the defatted algae tissue into water, so as to obtain a microalgae extract rich in nucleic acids, sugars, amino acids and inorganic salts; finally, concentrating the algae aqueous solution can help obtain a product dosage form that is easy to store and use.

[0035] In the present disclosure, the algae may be selected from Cryptophyceae, Chlorophyceae, Bacillariophyceae, Chrysophyceae, Macrophyceae, Euchlorophyceae, and the like.

[0036] In some examples, in step S10, the algae may be Pseudochlorophylla. In this case, Pseudochlorophylla contains abundant active ingredients, and the obtained algae extract can be enriched with active ingredients. In the present disclosure, when the algae is Pseudochlorophylla, the algae extract may also be referred to as a microalgae extract or a Pseudochlorophylla water extract.

[0037] In some examples, in step S10, the algae may be defatted using an organic solvent. In some examples, in step S10, defatting the algae may include extracting the algae using an organic solvent. Specifically, the algae may be mixed with an organic solvent, the fat in the algae may be dissolved in the organic solvent, and then filtered to obtain defatted algae tissue (referred to as defatted algae tissue). The filtrate obtained by filtration contains polyunsaturated fatty acids, which may be further processed to extract the polyunsaturated fatty acids for other applications. This can help improve the utilization rate of algae raw materials.

[0038] In some examples, in step S10, the algae is an algae with intact cell walls. The applicant has found that breaking the cell walls before defatting is not only unnecessary, but may also result in poor defatting effect. Therefore, in this embodiment, using algae with intact cell walls for defatting can help simplify the process and improve the extraction efficiency on the one hand, and also help remove fat in the algae as much as possible on the other hand.

[0039] In some examples, in step S10, the temperature for extracting the algae using the organic solvent (which may be referred to as the degreasing temperature) may be 60° C. to 90° C. Thus, it is easy to degrease the algae. In some examples, the degreasing temperature may be 60° C., 65° C., 70° C., 75° C., 80° C., 85° C., or 90° C. In some examples, preferably, the degreasing temperature may be 80° C.

[0040] In some examples, in step S10, the duration of extracting the algae using the organic solvent (which may be referred to as the defatting duration) may be 1 hour to 3 hours. Thus, it is convenient to defatt the algae. In some examples, the defatting duration may be 1 hour, 1.25 hours, 1.5 hours, 1.75 hours, 2 hours, 2.25 hours, 2.5 hours, 2.75 hours, or 3 hours.

[0041] In some examples, in step S10, the organic solvent may include any one or more combinations of ethanol, n-hexane, petroleum ether and n-heptane. In some examples, in step S10, preferably, the organic solvent may be ethanol, thereby facilitating efficient degreasing of algae.

[0042] In some examples, in step S10, the ratio of the organic solvent to the algae can be 2:1 to 10:1. This can help to efficiently defatting the algae. In some examples, the mass ratio of the organic solvent to the algae can be 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1 or 2:1.

[0043] In some examples, in step S10, the algae may be dry algae. Dry algae refers to algae with a moisture content of less than 5wt%. In this case, compared to wet algae, dry algae will not introduce too much water during the defatting process, which can help improve the extraction efficiency, and can help simplify the subsequent process of reusing the organic solvent, thereby helping to improve efficiency and reduce costs during large-scale preparation. Regarding the reuse of organic solvents, taking ethanol as an organic solvent as an example, ethanol can usually be recycled by distillation, but ethanol and water easily form azeotropes, and it is difficult to separate the water from ethanol by distillation alone. At this time, other means need to be introduced to separate the two, which is relatively complicated. Therefore, in this embodiment, the use of dry algae for defatting will not introduce too much water, which can help simplify the subsequent process of reusing the organic solvent.

[0044] In some examples, the original algae can be dried to obtain dry algae, and then the dry algae can be defatted. In other words, in some examples, the dry algae can be defatted. In some examples, the original algae can be flocculated and deflocculated and then dried to obtain dry algae. In this case, flocculation and deflocculation can remove impurities in the algae as much as possible, thereby improving the quality of the algae extract finally obtained. In some examples, the original algae can be flocculated by a flocculant. In some examples, the flocculant can be sodium hydroxide.

[0045] In some examples, when the algae is Nannochloropsis, since the culture environment of Nannochloropsis is usually in seawater, the original algae can be desalinated. In this case, the salt in the seawater can be removed, that is, the salt outside the algae cells can be removed, so that the inorganic salts in the algae extract finally obtained are natural salts released from the algae cells. In some examples, the original algae can be flocculated, deflocculated, desalted and dried to obtain dry algae.

[0046] In some examples, the number of times the algae is defatted may be a single time or multiple times. In some examples, preferably, the number of times the algae is defatted may be a single time. In a preferred embodiment of the present disclosure, selecting appropriate reaction conditions enables the step of defatting the algae to be performed only once, which can help simplify the process, thereby helping to improve efficiency and reduce costs during large-scale preparation. It is understandable that in order to further improve the purity of the target substance in the algae extract finally obtained, the algae may also be defatted multiple times. For example, the algae may be defatted 2, 3 or 4 times. Among them, the defatting time for each of the multiple defattings may be shorter than the defatting time for a single defatting.

[0047] In some examples, in step S10, the defatted algae tissue may be dried before the next step. In some examples, in step S10, the defatted algae tissue may be dried to make the defatted algae tissue in a dry powder form before water extraction. In the present disclosure, the defatted algae tissue in a dry powder form may be referred to as defatted algae powder. That is, in some examples, the defatted algae powder may be subjected to water extraction. In this case, the defatted algae tissue can be separated from the organic solvent containing lipids as much as possible, reducing the inconvenience caused by oil and fat in the subsequent processing process.

[0048] In some examples, as described above, in step S20, the defatted algae tissue may be subjected to water extraction. In step S20, the defatted algae tissue is subjected to water extraction to extract the target component in the defatted algae tissue by water. In some examples, in step S20, the defatted algae tissue may be boiled. Specifically, the defatted algae tissue may be mixed with water, and then the mixture may be heated to extract the target substance therein.

[0049] In some examples, in step S20, deionized water, distilled water, or tap water may be used for boiling. In some examples, tap water may be used for boiling. In this case, using tap water helps to reduce production costs in large-scale production compared to using deionized water or distilled water.

[0050] In some examples, in step S20, the mass volume ratio of the defatted algae tissue to water can be 5g:1L to 20g:1L. Thus, it can help to efficiently extract the defatted algae tissue. In some examples, the mass volume ratio of the defatted algae tissue to water can be 20g:1L, 19g:1L, 18g:1L, 17g:1L, 16g:1L, 15g:1L, 14g:1L, 13g:1L, 12g:1L, 11g:1L, 10g:1L, 9g:1L, 8g:1L, 7g:1L, 6g:1L or 5g:1L. In some examples, in step S20, preferably, the mass volume ratio of the defatted algae tissue to water is 10g:1L to 15g:1L.

[0051] In some examples, in step S20, the temperature for boiling the defatted algae tissue (which may be referred to as the water extraction temperature) may be 80°C to 100°C. Thus, it is possible to facilitate the dissolution of water-soluble substances in the defatted algae tissue in water. In some examples, the defatting temperature may be 80°C, 85°C, 90°C, 93°C, 95°C, 98°C or 100°C. In some examples, preferably, the defatting temperature may be 90°C to 100°C.

[0052] In some examples, in step S20, the duration of boiling the defatted algae tissue (which may be referred to as water extraction duration) may be 1 hour to 5 hours. Thus, it is convenient to dissolve the water-soluble substances in the defatted algae tissue in water. In some examples, the water extraction duration may be 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, 3.5 hours, 4 hours, 4.5 hours or 5 hours.

[0053] In some examples, the number of times the algae is water-extracted can be single or multiple times. In a preferred embodiment of the present disclosure, selecting appropriate reaction conditions enables the step of water-extracting the algae to be performed only once, which can help simplify the process, thereby helping to improve efficiency and reduce costs during large-scale preparation. It is understandable that in order to further increase the content of the target substance in the algae extract finally obtained, the algae can also be water-extracted multiple times. For example, the algae can be water-extracted 2 times, 3 times, or 4 times, and the duration of each water extraction in the multiple water extractions can be shorter than the duration of the water extraction in the single water extraction.

[0054] In some examples, as described above, in step S30, the algae aqueous solution may be concentrated to obtain an algae extract. In some examples, the concentrated algae extract may be in liquid form. Thus, the algae extract in the form of a concentrated liquid (which may be referred to as an algae extract concentrate) may be suitable for use in scenarios where liquid substances are required, and the algae extract concentrate is easier to store and use than an unconcentrated algae aqueous solution.

[0055] In some examples, in step S30, the method for concentrating the algae aqueous solution may be vacuum concentration. In some examples, the temperature of vacuum concentration may be 70°C to 100°C. Thus, it can help improve the concentration efficiency. In some examples, the temperature of vacuum concentration may be 70°C, 75°C, 80°C, 85°C, 90°C, 93°C, 95°C, 98°C, or 100°C.

[0056] In some examples, the vacuum concentration may be performed in a vacuum range of -0.05 MPa to -0.08 MPa. In some examples, an evaporator may be used for vacuum concentration. For example, a double-effect evaporator may be used for vacuum concentration.

[0057] In some examples, the preparation method may further include concentrating the algae aqueous solution and sterilizing the resulting product. Thus, a sterile algae extract may be obtained. In some examples, flash sterilization may be used for sterilization.

[0058] In some examples, the preparation method may further include concentrating the algae aqueous solution and drying the resulting product to obtain a powdered algae extract (which may be referred to as algae extract powder or algae extract dry powder). As a result, the algae extract can be suitable for scenarios where powdered substances are required, and the algae extract powder is easier to store and use than unconcentrated algae aqueous solutions. In some examples, spray drying can be used for drying. As a result, it can help to dry quickly and improve the overall preparation efficiency. In some examples, the inlet temperature of the spray drying can be 165°C to 180°C, and the outlet temperature can be 90°C to 100°C.

[0059] In some examples, the sterilized product can be dried. That is, in some examples, the algae aqueous solution can be concentrated, and then the resulting product can be sterilized and then dried. Thus, a sterile algae extract powder can be obtained.

[0060] In some examples, the dry matter content (i.e., solid content) in the algae extract obtained after vacuum concentration can be 0.01% to 20%. Thus, an algae extract with a predetermined concentration can be obtained. In some examples, the dry matter content in the algae extract can be 0.01%, 0.1%, 0.2%, 0.3%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% or 20%. The appropriate concentration can be selected according to the usage scenario.

[0061] In some examples, the algae extract prepared by the preparation method of the present disclosure may include 15% to 50% nucleic acid, 10% to 30% saccharide, 10% to 30% amino acid, and 5% to 30% inorganic salt. Among them, the saccharide may include polysaccharides and oligosaccharides. Thus, the preparation method of the present disclosure can produce an algae extract rich in a variety of beneficial substances. In some examples, the algae extract may also include 2% to 8% phosphorus. Thus, the content of beneficial factors in the algae extract can be further increased.

[0062] In some examples, preferably, the preparation method may include the following steps: defatting the algae: extracting the algae with an organic solvent, the extraction temperature is 60°C to 90°C, the duration is 1h to 3h, and defatted algae tissue is obtained; water extraction of the defatted algae tissue: boiling the defatted algae tissue in water, the boiling temperature is 80°C to 100°C, the duration is 1h to 5h, and an algae aqueous solution is obtained; and concentrating the algae aqueous solution to obtain an algae extract; wherein the algae is algae with intact cell walls, and the number of times of defatting the algae and extracting the defatted algae tissue is a single time. In this case, the appropriate reaction conditions are selected so that the steps of defatting the algae and extracting the defatted algae tissue only need to be performed once, thereby providing a preparation method with simple procedures and applicable to large-scale industrial production.

[0063] In some examples, the algae extract may include 15% to 50% nucleic acid. In some examples, the algae extract may include 10% to 30% carbohydrates. In some examples, the carbohydrates in the algae extract may include polysaccharides and oligosaccharides.

[0064] In some examples, the carbohydrate material may include any one or more of (1→3,1→4)-β glucan, anionic sulfated heterorhamnan, (1→3,1→4)-α mannan, and ribose. In some examples, among the carbohydrate materials, the sulfation degree of low molecular weight polysaccharides with a molecular weight below 10 KDa may be 5%-15%, and the sulfation degree of high molecular weight polysaccharides with a molecular weight above 10 KDa may be 40%-50%.

[0065] In some examples, the algae extract may include an amino acid content of 10% to 30%. In some examples, the algae extract may include an inorganic salt content of 5% to 30%.

[0066] In some examples, preferably, the algae extract may include 15% to 50% nucleic acid, 10% to 30% sugar, 10% to 30% amino acid, and 5% to 30% inorganic salt.

[0067] In some examples, the algae extract may further include 2% to 8% phosphorus, thereby further increasing the content of beneficial factors in the algae extract.

[0068] As mentioned above, the third aspect of the present disclosure relates to a cosmetic comprising an algae extract.

[0069] In some examples, preferably, the algae extract may include 15% to 50% nucleic acid, 10% to 30% saccharide, 10% to 30% amino acid, and 5% to 30% inorganic salt. In the present disclosure, it is verified that the synergistic effect of the components in the algae extract can make the algae extract have good anti-wrinkle, anti-aging, soothing, moisturizing and lightening fine lines and other effects, and it can be used as a functional component of cosmetics. Thus, cosmetics with good anti-wrinkle, anti-aging, soothing, moisturizing and lightening fine lines and other effects can be obtained.

[0070] In some examples, the algae extract may further include 2% to 8% phosphorus, thereby further increasing the content of beneficial factors in the algae extract.

[0071] In some examples, the carbohydrates in the algae extract may include polysaccharides and oligosaccharides.

[0072] In some examples, the carbohydrate substance may include any one or more of (1→3, 1→4)-β glucan, anionic sulfated heterorhamnan, (1→3, 1→4)-α mannan, and ribose.

[0073] In some examples, among carbohydrate substances, the sulfation degree of low molecular weight polysaccharides with a molecular weight below 10 KDa may be 5%-15%, and the sulfation degree of high molecular weight polysaccharides with a molecular weight above 10 KDa may be 40%-50%.

[0074] In some examples, in cosmetics, the concentration of algae extract can be 0.01%-0.5%. For example, the concentration of algae extract can be 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45% or 0.5%. Thus, it can help to obtain cosmetics with good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects.

[0075] In some examples, in the cosmetic, the concentration of the algae extract can be 0.01 g / L-5 g / L. For example, the concentration of the algae extract can be 0.01 g / L, 0.1 g / L, 0.5 g / L, 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L, 4 g / L, 4.5 g / L, or 0.5 g / L.

[0076] In some examples, the conductivity of the solution of the algae extract with a concentration of 0.3%-0.5% may be 600μS / cm-1500μS / cm. For example, the conductivity of the solution of the algae extract with a concentration of 0.3%-0.5% may be 600μS / cm, 680μS / cm, 700μS / cm, 800μS / cm, 900μS / cm, 1000μS / cm, 1100μS / cm, 1200μS / cm, 1300μS / cm, 1400μS / cm or 1500μS / cm. Thus, an algae extract with a suitable conductivity can be selected according to actual needs.

[0077] In some examples, the elastase inhibition rate of a solution of algae extract at a concentration of 0.1%-0.3% may be no less than 14%. In some examples, the elastase inhibition rate of a cosmetic containing algae extract at a concentration of 0.1%-0.3% may be no less than 14%.

[0078] In some examples, the solution of algae extract at a concentration of 2g / L-5g / L can promote the upregulation rate of type I collagen secretion by no less than 165%. In some examples, the cosmetics containing algae extract at a concentration of 2g / L-5g / L can promote the upregulation rate of type I collagen secretion by no less than 165%.

[0079] In some examples, the hyaluronidase inhibition rate of a solution of algae extract at a concentration of 0.2%-0.3% may be no less than 40%. In some examples, the hyaluronidase inhibition rate of a cosmetic containing algae extract at a concentration of 0.2%-0.3% may be no less than 40%.

[0080] In some examples, the 8h moisturizing rate of a solution of algae extract with a concentration of 0.05%-0.3% may be no less than 55%. In some examples, the 8h moisturizing rate of a cosmetic containing algae extract with a concentration of 0.05%-0.3% may be no less than 55%.

[0081] In some examples, the cosmetics may be in various forms known in the art. For example, the cosmetics may be in the form of emulsions, creams, ointments, gels, sprays, patches, and the like.

[0082] In some examples, the algae extract may be the only functional ingredient in the cosmetic. The only functional ingredient refers to the only ingredient that has a significant impact on the technical effect of the cosmetic. In some examples, the cosmetic may include excipients. In some examples, the excipients may include surfactants, flavors, pigments, preservatives, antioxidants, thickeners and / or acid-base regulators, etc.

[0083] In some examples, cosmetics may also include other known functional ingredients. For example, cosmetics may also include known components such as hyaluronic acid, glycerin, L-ascorbic acid, glycyrrhizin, arbutin salicylic acid, vitamin E, etc., which have anti-aging, moisturizing, and anti-oxidation effects. In some examples, algae extracts may be the main functional ingredients in cosmetics.

[0084] In the third aspect of the present disclosure, the algae extract may be prepared by the preparation method involved in the first aspect of the present disclosure, or may be prepared by other methods.

[0085] In some examples, preferably, the algae extract can be extracted from Pseudochlorophyll. Pseudochlorophyll contains abundant active ingredients, which can enrich the obtained algae extract with active ingredients. As mentioned above, in the present disclosure, when the algae is Pseudochlorophyll, the algae extract can be referred to as a microalgae extract.

[0086] In some examples, the algae extract can be diluted before use according to actual needs.

[0087] The fourth aspect of the present disclosure relates to the use of an algae extract in the preparation of an elastase-inhibiting preparation. The algae extract includes 15% to 50% nucleic acid, 10% to 30% carbohydrate, 10% to 30% amino acid, and 5% to 30% inorganic salt. In the present disclosure, it is verified that the synergistic effect of the components in the algae extract can have a significant inhibitory effect on elastase, and has good anti-wrinkle and fine line lightening effects. Specifically, elastase is mainly synthesized and secreted by fibroblasts. It is a highly selective and specific proteolytic enzyme that catalyzes the hydrolysis of carboxyl-containing polypeptide bonds of many amino acids such as glycine, leucine, alanine, and valine, and can decompose elastin in connective tissue protein and cause skin aging. In the present disclosure, the inhibition of elastase by algae extract can make the algae extract have good skin care effects such as anti-wrinkle and fine line lightening.

[0088] In some examples, the algae extract may further include 2% to 8% phosphorus, thereby further increasing the content of beneficial factors in the algae extract.

[0089] In some examples, the preparation can be food, cosmetics or medicine. The algae extract disclosed in the present invention is extracted from natural microalgae, which is a new type of food raw material with high biosafety and low risk of side effects, and can be applied to food or medicine.

[0090] In some examples, the algae extract can be used in an external manner. For example, the algae extract can be used as a functional component of a facial mask and an essence. In some examples, the algae extract can be used in an internal manner. For example, the algae extract can be used as a functional component of a functional capsule, a functional beverage, and a functional food.

[0091] In some examples, the preparation may also include auxiliary materials permitted by the regulations on cosmetics, ordinary foods, health foods and / or special medical foods. For example, the preparation may also include auxiliary materials such as thickeners, sweeteners, plasticizers and / or diluents.

[0092] The algae extract disclosed in the present invention is rich in natural beneficial ingredients such as nucleic acids, polysaccharides / oligosaccharides, amino acids and minerals, and has multiple skin care effects such as retaining moisture, regulating skin pH, anti-wrinkle, fading fine lines, and promoting cell proliferation. Therefore, the present invention can also provide an application of the algae extract in skin care, or an application in the preparation of a skin care preparation.

[0093] In summary, the present disclosure can provide a simple method for preparing algae extracts. The algae extracts are rich in beneficial substances such as nucleic acids, sugars, amino acids and inorganic salts, and have good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects, and can be used as functional components of cosmetics.

[0094] In order to further illustrate the present disclosure, the preparation method and application of the microalgae extract provided by the present disclosure are described in detail below in conjunction with the examples, and the beneficial effects achieved by the present disclosure are fully illustrated in conjunction with the comparative examples.

[0095] It should be noted that, in the examples of the present disclosure, unless otherwise specified, the reagents and instruments used are all commercially available products.

[0096] [Example 1]

[0097] 1. Preparation of algae extract

[0098] (1) treating the Pseudochlorophyll algae: firstly adjusting the pH to about pH=10.5 with a flocculant (sodium hydroxide) to flocculate the algae from the liquid phase; then collecting the algae by centrifugation or filtration, and then adjusting the pH to about pH=7 to deflocculate, remove salt, and then drying to obtain Pseudochlorophyll algae powder with a water content of less than 5wt%;

[0099] (2) adding 40 kg of Nannochloropsis algae powder to 300 kg of 94% ethanol, heating and maintaining a slightly boiling state (80° C.) for 3 hours, cooling to room temperature, filtering through a filter bag, and drying to obtain about 10 kg of defatted algae powder;

[0100] (3) adding 10 kg of defatted algae powder to 1000 L of tap water, heating to 93 ° C and maintaining for 2 hours, cooling and filtering to obtain an algae aqueous solution;

[0101] (4) The algae aqueous solution is added to a double-effect evaporator at an evaporation temperature of 70° C. and a vacuum range of -0.05 to -0.08 MPa, concentrated to a water extract solution with a dry matter content of 5%, and sterilized to obtain an algae extract.

[0102] 2. Analysis of components of algae extract

[0103] (1) The polysaccharide / oligosaccharide content in the water extract measured by SN / T4260 was 25.41%; the total amino acid content measured by GB5009.124 was 24.04%; the ash content measured by GB5009.4 was 23.15%; the phosphorus content measured by GB / T5537 was 51.76 mg / g;

[0104] (2) A 1% algae extract solution was prepared, and the pH value was measured to be 6.03; wherein, "1% algae extract solution" refers to an algae extract solution with a solid content of 1%. Other identical expressions in the present disclosure have the same meaning and are not repeated here.

[0105] (3) A 0.2% algae extract solution was prepared, and the absorption value at 230 nanometers, 260 nanometers, and 280 nanometers was measured by a UV spectrophotometer to be 0.904, 1.956, and 0.972. A260 / A230 = 2.16, A260 / A280 = 2.01, both of which are greater than two, indicating that there are nucleic acid substances in the solution. According to the commonly used empirical nucleic acid content calculation formula in the industry, the nucleic acid content is about A260*50 / 200 = 48.9%, of which the nucleic acid base is about 48.9%*130 / 362 = 17.56%.

[0106] The above results indicate that the main components of algae extracts are polysaccharides / oligosaccharides, amino acids, inorganic salts and nucleic acids, and that their aqueous solutions are weakly acidic and have the effects of retaining skin moisture and regulating skin pH.

[0107] [Example 2]

[0108] An algae aqueous solution is prepared in the same manner as steps (1) to (3) in Example 1; the algae aqueous solution is added to a double-effect evaporator at an evaporation temperature of 70°C and a vacuum range of -0.05 to -0.08 MPa, concentrated to an aqueous extract solution with a dry matter content of 10%, sterilized, and spray-dried (inlet air temperature to 165-180°C, outlet air temperature to 90-100°C) to obtain a light yellow algae extract powder.

[0109] Evaluation of the efficacy of algae extracts

[0110] 1. Inhibition rate of elastase

[0111] The principle of the elastase inhibition experiment is that elastase reacts with the enzyme substrate, and the absorbance changes after adding the active substance. The magnitude of the absorbance change can reflect the inhibition rate of the elastase inhibitor. The specific operation is as follows:

[0112] An appropriate amount of buffer solution, sample solutions or control solutions of different concentrations, and substrate were added to a 96-well plate, and the mixture was shaken and incubated for 20 minutes. Then, the elastase solution was added, and the mixture was immediately shaken and incubated for 10 minutes. The absorbance was measured at 410 nm using an enzyme reader, and the data was analyzed and processed to calculate the inhibition rate of the sample to be tested on elastase. The results are shown in Table 1.

[0113] Table 1

[0114]

[0115] Note: p < 0.05 indicates a statistically significant difference compared with the negative control group, and p ≥ 0.05 indicates no statistically significant difference compared with the negative control group.

[0116] As shown in Table 1, when the concentration of the positive control group was 200 μg / mL, the inhibition rate of elastase was ≥90%, indicating that the reaction system was effective; the inhibition rate of elastase of 0.1% algae extract solution was 14.16%; the inhibition rate of elastase of 0.2% algae extract solution was 16.74%; the inhibition rate of elastin of 0.3% algae extract solution was 20.17%. As the concentration of the algae extract solution increased, the inhibition rate of elastase also gradually increased, indicating that the synergistic effect of the various components in the pseudoalgae extract can have a significant inhibitory effect on elastase and has anti-wrinkle effect.

[0117] 2. Upregulation rate of type I collagen

[0118] Type I collagen is one of the main components of the extracellular matrix of dermis. Type I collagen secreted by fibroblasts aggregates extracellularly to form collagen fibers, which can stabilize and pull the skin and improve the firmness of the skin. Using human dermal fibroblasts as a model, the type I collagen content in the solvent control group and the test substance administration experimental group was determined by enzyme-linked immunosorbent assay (ELISA) to evaluate whether the test substance has an effect on promoting the synthesis and secretion of type I collagen, so as to evaluate whether the test substance has anti-wrinkle effect.

[0119] 2.1. Cytotoxicity - Confirmation of Maximum Safe Dosing Concentration

[0120] Cell inoculation: Take cells in the logarithmic growth phase and inoculate them into a 96-well plate. Place the culture plate at 37°C ± 1°C and CO2 concentration of 5% ± 1% for 24 hours.

[0121] Solution preparation and grouping: Prepare the working solution of the test substance according to Table 2. The test is divided into a solvent control group, a sample group, a positive control group and a blank group (zero adjustment, no cells).

[0122] Table 2

[0123]

[0124] Dosing: Add samples, positive controls, solvent controls, and blank controls to a 96-well plate, with a dosing volume of 100 μL per well, and 3 replicates per group. After dosing, place the 96-well plate at 37°C ± 1°C and a CO2 concentration of 5% ± 1% for incubation.

[0125] Detection: Incubate the cells for 24 hours, and discard the supernatant after washing. Add DMEM complete medium and CCK-8 solution to each well, place in a carbon dioxide incubator, and culture for about 90 minutes. Measure the absorbance (OD) value at a wavelength of 450nm.

[0126] The results are shown in Table 3. During the test, the SD values ​​of cell viability in the three parallel wells of the test substance were all less than 15%, which met the test requirements.

[0127] Table 3

[0128]

[0129] According to the data in Table 3 and combined with the cell morphology pictures, the final dosage concentrations were determined to be 5 g / L, 3 g / L, and 2 g / L.

[0130] 2.2. Determination of type I collagen content

[0131] Concentration determination: After the cells are incubated with different concentrations of the test sample for 24 hours, if there is no significant change in cell morphology compared with the solvent control, the sample is considered to have no toxic effect on the cells at this concentration. The test concentration was finally determined based on the cell morphology and cytotoxicity data, see Table 4.

[0132] Cell inoculation: Cells in the logarithmic growth phase were inoculated into a 96-well plate, and the culture plate was placed at 37°C ± 1°C and a CO2 concentration of 5% ± 1% for 24 hours.

[0133] Solution preparation: Prepare the test substance working solution according to Table 4.

[0134] Table 4

[0135]

[0136] Drug induction: Add the working solution of the test substance to a 96-well plate, with a dosage of 100 μL per well; set up a solvent control and a positive control in parallel. Set up 6 replicate wells for each group. Place the culture plate at 37°C ± 1°C and a CO2 concentration of 5% ± 1% for 24 hours.

[0137] Collection of cell supernatant solution: After 24 hours of culture, collect a sufficient amount of cell supernatant, divide it into portions, and store it in a -80°C ultra-low temperature refrigerator.

[0138] ELISA test: Follow the instructions of the ELISA kit to determine the content of type I collagen. Before the formal test, the sample dilution ratio should be set, and a preliminary experiment should be conducted to explore and determine the dilution ratio of the experimental test group to ensure that the ELISA test value falls within the standard curve range.

[0139] The software "CurveExpeti" and "IBMSPSSStatistics26" were used for data analysis and processing. The T-test method was used for analysis between the groups. When P < 0.05, it indicated a significant difference (indicated by *), and when P < 0.01, it indicated an extremely significant difference (indicated by **).

[0140] The detection results of human dermal fibroblasts are shown in Table 5.

[0141] Table 5

[0142]

[0143] The results showed that compared with the BC group, the PC group significantly promoted the secretion of type I collagen (P<0.01), and the experimental system was established. Compared with the BC group, the algae extract significantly promoted the secretion of type I collagen at concentrations of 5g / L, 3g / L, and 2g / L (P<0.01), with the upregulation rates being 301.36%, 232.76%, and 165.84%, respectively.

[0144] 3. Anti-aging effect

[0145] The anti-aging effect of algae extracts was studied using the zebrafish anti-aging effect test method, and the operation was carried out in accordance with the "Standard Operating Procedures for Zebrafish Anti-Aging Effect Evaluation Experiment".

[0146] Principle and method: Peroxides enter the body to produce free radicals, causing lipid peroxidation, thereby destroying the stability of biological membranes, producing lipofuscin, damaging nuclear DNA and mitochondrial DNA, causing protein oxidative damage and macromolecular cross-linking, thereby inducing aging. β-galactosidase (SA-β-gal) is a marker of cell aging. In aging cells, β-galactosidase activity increases and expression increases. Therefore, the average value of β-galactosidase staining intensity is detected to evaluate whether the sample has an anti-aging effect.

[0147] System and sample size

[0148] Experimental system: Wild-type AB strain.

[0149] Zebrafish age: 6 hours post fertilization (6hpf).

[0150] The sample size of each experimental group was 15 (N=10).

[0151] Adult fish rearing and breeding methods: The rearing and breeding methods meet the requirements of the international AAALAC certification (certification number: 001458).

[0152] Experimental procedures

[0153] 1. Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish in each well.

[0154] 2. Establish a zebrafish aging model by administering water-soluble hydrogen peroxide.

[0155] 3. The water-soluble samples were administered, and a normal control group and a model control group were set up at the same time. The capacity of each well was 3 mL.

[0156] 4. Change the liquid once a day and incubate at 28℃ in the dark for 120 hours.

[0157] 5. The zebrafish were fixed with 4% cell tissue fixative overnight and stained with a β-galactosidase staining kit. After staining overnight, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. The data were analyzed and collected using advanced image processing software. The average value (S) of the zebrafish β-galactosidase staining intensity was analyzed, and the formula was used to calculate and determine whether the sample had an anti-aging effect. Anti-aging effect (%) = (S (model control group) - S (sample group)) / (S (model control group) - S (normal control group)) * 100%.

[0158] The test results are shown in Table 6.

[0159] Table 6

[0160]

[0161] The sample action experimental phenotype diagram, such as Figure 2 As shown, Figure 2 is a typical anti-aging diagram involved in the examples of this disclosure, in Figure 2 In the figure, the red dotted line is the quantitative area.

[0162] It was observed that the average β-galactosidase staining intensity of the sample group of the N. oculiformis extract was significantly reduced compared with the model control group, revealing that the sample has an anti-aging effect. In summary, the sample N. oculiformis extract has an anti-aging effect.

[0163] 4. Hyaluronic acid inhibition rate

[0164] Hyaluronic acid is an important component of the extracellular matrix of the skin and plays an important role in maintaining the volume of the extracellular matrix and the normal physiological function of the skin. Hyaluronidase is an enzyme that can hydrolyze hyaluronic acid and is associated with most IgE-mediated type I and T-cell-mediated type IV allergic reactions. In addition, the hydrolysis products of hyaluronic acid promote skin inflammatory reactions. Inhibiting the activity of hyaluronidase can ensure the normal content and function of hyaluronic acid in the skin.

[0165] This test method is an in vitro method, which mainly characterizes the soothing effect by evaluating the inhibition rate of the test sample on hyaluronidase activity. It is suitable for evaluating cosmetics that achieve soothing effects by inhibiting hyaluronidase activity.

[0166] Test process: add enzyme and CaCl2 to the reaction tube, treat at 37℃ for 20min, add sample, treat at 7℃ for 20min, add hyaluronate solution, add acetylacetone and sodium hydroxide solution, boil in water bath and then ice bath, add color developer, measure absorbance at 530nm, the results are shown in Table 7.

[0167] The calculation formula is: Hyaluronidase inhibition rate (%) = [1-(AB) / (CD)] × 100%.

[0168] Where: A is the absorbance of the experimental group, B is the absorbance of the experimental blank group, C is the absorbance of the control group, and D is the absorbance of the blank group.

[0169] Table 7

[0170]

[0171]

[0172] Note: p < 0.05 indicates a statistically significant difference compared with the negative control group, and p ≥ 0.05 indicates no statistically significant difference compared with the negative control group.

[0173] According to the test results: when the concentration of the positive control group was 5 mg / mL, the hyaluronidase inhibition rate was ≥ 90%, indicating that the reaction system was effective;

[0174] When the concentration of the test sample group was 0.2% (v / v), the hyaluronidase inhibition rate was approximately 42.55%; when the concentration was 0.3% (v / v), the hyaluronidase inhibition rate was approximately 57.45%, which was statistically different from that of the negative control group (p < 0.05), indicating that the water extract of Pseudochlorophyll has the ability to inhibit hyaluronidase activity at the tested concentration, which can be used as one of the evidences to support the claim that the sample has a soothing effect.

[0175] 5. Moisturizing rate

[0176] According to the difference in moisturizing properties of cosmetic ingredients, different moisturizer molecules have different forces on water molecules, and their ability to absorb and retain water is also different. Oil has a sealing effect on water, which can prevent water loss. The one with a strong hygroscopic effect has a strong binding force on water molecules, and absorbs and moisturizes a large amount of water. The one with good sealing properties has less water loss.

[0177] This test method places the sample under constant temperature and humidity conditions, continuously monitors the change in water weight of the test sample, and plots a curve of the test sample's moisture retention rate versus time. The moisture retention capacity of the test sample is observed based on the trend of the moisture retention rate change curve, and the moisturizing effect of the test sample on the skin is evaluated.

[0178] Use a weighing bottle as a carrier to weigh 1g of sample (algae extract, accurate to 0.0001g), select a reagent with known moisturizing ability as a positive control (5% glycerol aqueous solution), record the mass of the weighing bottle and the mass of the sample, place the sample and the positive control in an environment of (22±2)℃ and humidity (50±10)%, weigh and record the data at intervals, calculate the moisturizing rate, and draw a change curve. The results are shown in Table 8. Figure 3 is a graph showing the moisture retention rates of the samples according to the examples of the present disclosure.

[0179] Table 8

[0180]

[0181]

[0182] According to the test results:

[0183] When the concentration of the tested sample was 0.05%, the moisture retention rates of the tested sample at 1h and 2h were 94.07% and 87.48% respectively, which were better than the positive control, indicating that the tested sample had a moisturizing effect under this experimental condition; the moisture retention rates at 4h and 8h were 76.28% and 56.12%;

[0184] When the concentration of the tested sample was 0.1%, the moisturizing rates of the tested sample at 1h and 2h were 94.48% and 87.76% respectively, which were better than the positive control, indicating that the tested sample had a moisturizing effect under this experimental condition; the moisturizing rates at 4h and 8h were 76.95% and 57.01%;

[0185] When the concentration of the tested sample was 0.2%, the moisturizing rates of the tested sample at 1h, 2h and 4h were 94.92%, 88.08% and 77.86% respectively, which were better than the positive control, indicating that the tested sample had a moisturizing effect under this experimental condition; the moisturizing rate at 8h was 57.79%;

[0186] When the concentration of the tested sample was 0.3%, the moisturizing rates of the tested sample at 1h, 2h and 4h were 95.22%, 88.13% and 78.20% respectively, which were better than the positive control, indicating that the tested sample had a moisturizing effect under the experimental conditions; the moisturizing rate at 8h was 58.33%.

[0187] 6. Evaluation of the efficacy of algae extract in reducing fine lines

[0188] Prepare a 0.1% algae extract solution, take 1 mL and apply it around the eyes of the subjects, and observe the fine lines at the corners of the eyes of the subjects after 5 minutes. Figure 4 and Figure 5 As shown, Figure 4 is a schematic diagram showing the periorbital area of ​​a subject before administration of an algae extract according to an embodiment of the present disclosure, Figure 5 is a schematic diagram showing the periorbital area of ​​a subject after administration of an algae extract in accordance with an embodiment of the present disclosure.

[0189] from Figure 4 and Figure 5 It can be seen that algae extract can significantly reduce fine lines at the corners of the eyes.

[0190] 7. Conductivity of algae extract

[0191] The conductivity of the algae extract solution was tested using a conductivity meter, and the results are shown in Table 9.

[0192] Table 9

[0193] Experimental concentration Conductivity (μS / cm) 0.30％ 689 0.50％ 1428

[0194] In summary, the present disclosure can provide a simple method for preparing algae extracts, which is suitable for large-scale industrial production. The prepared microalgae extracts are rich in beneficial ingredients such as nucleic acids, sugars, amino acids and inorganic salts, and can be used in fields such as food and cosmetics. In particular, the present disclosure also verifies that the algae extracts have good anti-wrinkle, anti-aging, soothing, moisturizing and fine line lightening effects, and can be used as functional components of cosmetics.

[0195] Although the present disclosure is specifically described above in conjunction with the accompanying drawings and examples, it is to be understood that the above description does not limit the present disclosure in any form. Those skilled in the art may modify and change the present disclosure as needed without departing from the essential spirit and scope of the present disclosure, and these modifications and changes all fall within the scope of the present disclosure.

Claims

1. A method for preparing an algae extract, characterized in that: The following steps are involved: Degreasing the algae: extracting the algae with an organic solvent at a temperature of 60° C. to 90° C. for 1 to 3 hours to obtain defatted algae tissue; Extracting the defatted algae tissue with water: boiling the defatted algae tissue with water at a temperature of 80° C. to 100° C. for 1 to 5 hours to obtain an algae aqueous solution; and Concentrating the algae aqueous solution to obtain an algae extract; The algae has intact cell walls, and the algae extract comprises 15% to 50% nucleic acid, 10% to 30% sugars, 10% to 30% amino acids, and 5% to 30% inorganic salts.

2. The preparation method according to claim 1, characterized in that: The organic solvent comprises any one or more combinations of ethanol, n-hexane, petroleum ether and n-heptane, and the mass ratio of the organic solvent to the algae is 2:1 to 10:

1.

3. The preparation method according to claim 1, characterized in that: The defatted algae tissue is dried to be in the form of dry powder and then water extraction is performed.

4. The preparation method according to claim 1, characterized in that: When the defatted algae tissue is subjected to water extraction, the mass volume ratio of the defatted algae tissue to water is 5 g:1 L to 20 g:1 L.

5. The preparation method according to claim 1, characterized in that: The method for concentrating the algae aqueous solution is vacuum concentration, and the temperature of vacuum concentration is 70° C. to 100° C.

6. The preparation method according to claim 1, characterized in that: The method also includes concentrating the algae aqueous solution and drying the obtained product to obtain the algae extract in powder form.

7. The preparation method according to claim 1, characterized in that: The algae is Nannochloropsis spp.

8. An algae extract, characterized in that The algae extract is prepared by any one of the preparation methods described in claims 1-7.

9. A cosmetic, characterized in that: The algae extract comprises 15% to 50% of nucleic acid, 10% to 30% of sugar, 10% to 30% of amino acid and 5% to 30% of inorganic salt.

10. The cosmetic according to claim 9, characterized in that: In the cosmetic, the concentration of the algae extract is 0.01%-0.5%.

11. The cosmetic according to claim 9, characterized in that: The conductivity of the solution of the algae extract at a concentration of 0.3% to 0.5% is 600 μS / cm to 1500 μS / cm.

12. The cosmetic according to claim 9, characterized in that: The elastase inhibition rate of the solution of the algae extract with a concentration of 0.1%-0.3% is not less than 14%; the up-regulation rate of the type I collagen secretion promoted by the solution of the algae extract with a concentration of 2g / L-5g / L is not less than 165%; the hyaluronidase inhibition rate of the solution of the algae extract with a concentration of 0.2%-0.3% is not less than 40%; the 8h moisturizing rate of the solution of the algae extract with a concentration of 0.05%-0.3% is not less than 55%.

13. The cosmetic according to claim 9, characterized in that: The carbohydrate substances include any one or more of (1→3,1→4)-β glucan, anionic sulfated heterorhamnose, (1→3,1→4)-α mannan, and ribose, and among the carbohydrate substances, the sulfation degree of low molecular weight polysaccharides with a molecular weight lower than 10 KDa is 5%-15%, and the sulfation degree of high molecular weight polysaccharides with a molecular weight higher than 10 KDa is 40%-50%.

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