Cacumen biotae fermentation filtrate, caviar and Cacumen biotae nano-coated slow-release composition, shampoo and application

Abstract

The invention relates to the technical field of daily chemicals, in particular to a cacumen biotae fermentation filtrate, a caviar and cacumen biotae nano-coated slow-release composition, shampoo and application. The fermentation filtrate is prepared from cacumen biotae through a liquid fermentation method; wherein the used strain is bacillus and / or aspergillus. The cacumen biotae fermented filtrate provided by the invention contains flavonoid compounds (such as quercetin) and volatile oil (such as cacumen biotae ketone) which can improve scalp blood circulation, nourish hair follicles and promote healthy growth of hair. The shampoo can inhibit hair follicle inflammation and prevent hair follicle atrophy, thereby reducing alopecia. Tannin components can balance grease secretion of scalp, and hair follicle blockage caused by excessive grease is avoided. The shampoo is particularly friendly to oily scalp and is beneficial to keeping the scalp fresh and cool. Polysaccharide and flavonoid substances in the cacumen biotae can prolong the hair growth period and stimulate the activity of hair follicles, so that new hair regeneration is promoted. The plant extract mildly stimulates scalp and does not damage hair follicle tissues.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, in particular to a Platycladus orientalis leaf fermentation filtrate, a caviar Platycladus orientalis leaf nano-encapsulated sustained-release composition, a shampoo and applications. Background Art

[0002] Platycladi Cacumen is the dried branches and leaves of Platycladus orientalis (L.) Franco, a plant of the Cupressaceae family. It is commonly used in traditional Chinese medicine and hair and skin care products. It has attracted extensive attention due to its unique chemical composition and multiple pharmacological effects. Platycladi Cacumen contains a wealth of active chemical components, (1) volatile oils: thujone, thujol, camphene. (2) flavonoids: quercetin, isoquercitrin. (3) tannins; (4) polysaccharides; (5) other components: alkaloids, amino acids.

[0003] Androgenic alopecia (AGA): The most common type of hair loss in both men and women, commonly known as "Mediterranean" or "baldness". Genetics determine the sensitivity of hair follicles to androgens (DHT), which causes the hair follicles to gradually shrink, the hair becomes thinner, and even falls out. Hyperthyroidism or hypothyroidism can affect the hair growth cycle, leading to diffuse hair loss. Hair is made of keratin, and a lack of protein may cause hair to grow slowly or even fall out.

[0004] Anti-hair loss shampoo is a shampoo product specially designed to reduce hair loss and promote hair health. It achieves the anti-hair loss effect by regulating the scalp environment, improving the condition of hair follicles, and stimulating hair regeneration. Anti-hair loss shampoo gently removes dirt, oil and cuticles to keep the scalp fresh. Reduce the blockage of hair follicles to avoid hair loss caused by excessive oil. Regulate the scalp microenvironment, provide nutrients that promote hair follicle activity, and prolong the hair growth period. Promote hair regeneration by improving blood circulation and directly stimulating hair follicles.

[0005] The invention provides a fermentation filtrate of Platycladus orientalis leaves, a composition, a shampoo and an application thereof. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a fermentation filtrate of Platycladus orientalis leaves, wherein the fermentation filtrate is prepared by liquid fermentation of Platycladus orientalis leaves; wherein the fungi used are: Bacillus and / or Aspergillus.

[0007] As an embodiment of the present invention, the bacteria used in the fermentation are Bacillus subtilis, Bacillus cereus and Aspergillus.

[0008] The second aspect of the present invention provides a caviar-Platus orientalis leaf nano-encapsulated sustained-release composition, comprising the Platus orientalis leaf fermentation filtrate, caviar and silicon dioxide.

[0009] As an embodiment of the present invention, the specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm.

[0010] The present invention provides a method for preparing the composition, the steps of which are as follows:

[0011] S01 Dissolve caviar powder in deionized water, use a stirrer, start stirring for 0.5h, and set aside;

[0012] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0013] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0014] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0015] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0016] S06: Dry the obtained product to obtain a composite material.

[0017] As an embodiment of the present invention, the silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0018] The present invention provides a shampoo, and the raw materials for its preparation include, in parts by mass:

[0019] A solvent, a composition as claimed in claim 3, a surfactant, a thickener, a preservative, a moisturizer, an antidandruff agent, a pH adjuster, a chelating agent, a fragrance, and a hair conditioner.

[0020] As an embodiment of the present invention, the surfactant comprises sodium laureth sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, sodium lauroyl glutamate, lauryl diethylenediaminoglycine, and ammonium lauryl sulfate.

[0021] As an embodiment of the present invention, the hair conditioner comprises ginseng root extract, adenosine, allantoin and creatine.

[0022] The Platycladus orientalis leaf fermentation filtrate provided by the invention is applied to the field of daily chemicals.

[0023] By adopting the above technical solution, the present invention has the following beneficial effects:

[0024] The fermented filtrate of Platycladus orientalis leaves provided by the present invention contains flavonoids (such as quercetin) and volatile oils (such as thujone), which can improve scalp blood circulation, nourish hair follicles, and promote healthy hair growth. It can inhibit hair follicle inflammation, prevent hair follicle atrophy, and thus reduce hair loss.

[0025] Tannin ingredients can balance the oil secretion of the scalp and avoid clogging of hair follicles caused by excessive oil. It is especially friendly to oily scalps and helps keep the scalp fresh.

[0026] The polysaccharides and flavonoids in Platycladus orientalis leaves can prolong the growth phase of hair and stimulate the activity of hair follicles, thereby promoting the regeneration of new hair. The plant extracts gently stimulate the scalp without damaging the hair follicle tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 This is a picture of the caviar Platycladus orientalis leaf nano-encapsulated sustained-release composition. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The invention provides a fermentation filtrate of Platycladus orientalis leaves. The fermentation filtrate is prepared by liquid fermentation of Platycladus orientalis leaves. The fungi used are Bacillus and / or Aspergillus.

[0031] The best method for preparing the Platycladus orientalis leaf fermentation filtrate is as follows:

[0032] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0033] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0034] Among them, the bacterial species are Bacillus subtilis, Bacillus cereus and Aspergillus, and the inoculation ratio is 4:2:2.

[0035] Fermentation conditions: temperature: 30-37°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0036] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0037] The second aspect of the present invention provides a composition comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0038] As an embodiment of the present invention, the specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0039] The present invention provides a method for preparing the composition, the steps of which are as follows:

[0040] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0041] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0042] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0043] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0044] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0045] S06: Dry the obtained product to obtain a composite material.

[0046] As an embodiment of the present invention, the silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0047] The present invention provides a shampoo, and the raw materials for its preparation include, in parts by mass:

[0048] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0. 9:0.1:0.1) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; EDTA disodium 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; water is added to 100 parts.

[0049] The flavonoids in Platycladus orientalis leaves have antioxidant, anti-inflammatory and blood circulation promoting effects, which can improve scalp microcirculation and provide more nutrition for hair follicles. Among the flavonoids, thujone is the priority. Amentoflavone is a unique biflavonoid compound with multiple biological activities such as antioxidant, antibacterial and anti-inflammatory, which helps to improve the hair follicle environment. However, its low solubility in water limits its direct application; at the same time, thujone is easily decomposed by light or oxidants, so the extract needs to be stored away from light.

[0050] In addition, the tannin components in Platycladus orientalis leaves have high antioxidant activity and have a protective effect on hair follicles and scalp cells; its water solubility is more ideal than that of flavonoids. Therefore, conventional water extraction or solvent extraction has the disadvantages of incomplete extraction, easy destruction at high temperature, and different polarity; the present invention uses a fermentation method for extraction. However, the main components are mainly concentrated in the epidermis and glandular tissues of Platycladus orientalis leaves, which are the key sources of the antioxidant and antibacterial capabilities of Platycladus orientalis leaves. For this reason, the present invention uses two types of Bacillus for fermentation treatment, which can secrete cellulase, protease and pectinase, degrade plant cell walls, decompose proteins, and make glandular destruction more thorough. In addition, the addition of Aspergillus can produce protein and glycolipid surfactants, which have a stronger adsorption effect with silicon dioxide in the later preparation process of the composition; but after the silicon dioxide is treated, it can be parsed due to the effects of friction and electrostatic force during use, achieving better absorption.

[0051] In addition, the culture medium is regulated during fermentation to extend the fermentation period and release more flavonoid components; the metabolic pathways of flavonoid precursors (such as phenylalanine and lignin) in Platycladus orientalis leaves are activated; and the effective content of the prepared Platycladus orientalis leaf extract is higher.

[0052] In addition, the present invention takes into account the water solubility and ultraviolet instability of the Platycladus orientalis leaf extract, and integrates Platycladus orientalis leaf, caviar powder and silicon dioxide to further improve its stability and scalp absorbency after friction; however, after integration into silicon dioxide spheres, its suspension needs to be adjusted; therefore, on the one hand, the inoculation amount of Aspergillus is increased during fermentation; on the other hand, multiple ingredients are provided when preparing shampoo to ensure its suspension, for example, the addition of ingredients such as cyclodextrin, maltodextrin, cocamidopropyl betaine, guar hydroxypropyltrimethylammonium chloride, etc., can achieve better suspension.

[0053] The present invention is further explained below in conjunction with specific implementation modes.

[0054] Example 1

[0055] The present embodiment provides a Platycladus orientalis leaf extract, and the preparation method thereof is as follows: the Platycladus orientalis leaves are dried at low temperature, crushed by submerging, extracted with distilled water at a solid-liquid ratio of 1:10, the extract temperature is 90 degrees Celsius, the time is 3 hours, and this operation is repeated 3 times. The three extracts are collected and concentrated by reduced pressure evaporation to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf extract.

[0056] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf extract, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf extract, caviar and silicon dioxide is 1:1:8.

[0057] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0058] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0059] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0060] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0061] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0062] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the Platycladus orientalis leaf extract into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0063] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0064] S06: Dry the obtained product to obtain a composite material.

[0065] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0066] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0067] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0068] Example 2

[0069] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0070] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0071] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0072] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290), and the inoculum ratio was 1:1.

[0073] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0074] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0075] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0076] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0077] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0078] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0079] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0080] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0081] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0082] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0083] S06: Dry the obtained product to obtain a composite material.

[0084] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0085] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0086] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0087] Example 3

[0088] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0089] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0090] (2) Preparation of culture medium: 30 g of glucose, 5 g of yeast extract, 3 g of ammonium sulfate, 2 g of potassium dihydrogen phosphate, 0.5 g of magnesium salt (MgSO4·7H2O), 0.01 g of iron salt (FeSO4·7H2O), 50 g of Platycladus orientalis leaf powder, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0091] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290) and Aspergillus (Aspergillus 40223), and the inoculum ratio was 4:2:2.

[0092] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0093] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0094] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0095] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0096] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0097] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0098] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0099] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0100] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0101] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0102] S06: Dry the obtained product to obtain a composite material.

[0103] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0104] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0105] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0106] Example 4

[0107] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0108] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0109] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0110] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290) and Aspergillus (Aspergillus 40223), and the inoculum ratio was 4:3.5:0.5.

[0111] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0112] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0113] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0114] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0115] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0116] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0117] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0118] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0119] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0120] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0121] S06: Dry the obtained product to obtain a composite material.

[0122] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0123] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0124] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate, and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture 1 part of 1,4-dimethicone (mass ratio is 72.9:10:8:8:0.9:0.1:0.1); 0.8 part of sodium chloride; 0.5 part of niacinamide; 0.4 part of phenoxyethanol; 0.35 part of taurine; 0.3 part of sodium benzoate; 0.3 part of adenosine; 0.17 part of allantoin; 0.03 part of creatine; 0.2 part of panthenol; 0.15 part of piroctone olamine salt; 0.12 part of citric acid; 0.1 part of disodium EDTA; 0.5 part of (daily use) flavor; 0.003 part of ginsenoside; and add water to 100 parts.

[0125] Example 5

[0126] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0127] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0128] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0129] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290) and Aspergillus (Aspergillus 40223), and the inoculum ratio was 4:2:2.

[0130] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0131] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0132] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0133] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , pore size is 19.04533nm, particle size is 10-20nm.

[0134] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0135] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0136] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0137] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0138] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0139] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0140] S06: Dry the obtained product to obtain a composite material.

[0141] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0142] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0143] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0144] Example 6

[0145] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0146] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0147] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0148] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290) and Aspergillus (Aspergillus 40223), and the inoculum ratio was 4:2:2.

[0149] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0150] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0151] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0152] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0153] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0154] The composition can be obtained by simply mixing and stirring the fermented filtrate of Platycladus orientalis leaves, caviar and silicon dioxide.

[0155] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0156] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0157] Example 7

[0158] This embodiment provides a fermentation filtrate of Platycladus orientalis leaves, and the preparation method of the fermentation filtrate of Platycladus orientalis leaves is as follows:

[0159] (1) Select fresh, mildew-free Platycladus orientalis leaves, preferably leaves picked from areas with good growth and no pollution. Rinse off the dirt and impurities on the surface with clean water. Dry at low temperature (temperature controlled below 50°C). Grind the dried Platycladus orientalis leaves into coarse powder and sieve.

[0160] (2) Preparation of culture medium: glucose: 30 g, yeast extract: 5 g, ammonium sulfate: 3 g, potassium dihydrogen phosphate: 2 g, magnesium salt (MgSO4·7H2O): 0.5 g, iron salt (FeSO4·7H2O): 0.01 g, Platycladus orientalis leaf powder 50 g, starch hydrolyzate: 1 g, glutamic acid: 0.1 g, add water to 500 mL; inoculate the bacteria, inoculation amount: 8%.

[0161] Among them, the strain is Bacillus subtilis (Bacillus subtilis 10732), Bacillus cereus 21290) and Aspergillus (Aspergillus 40223), and the inoculum ratio was 4:2:2.

[0162] Fermentation conditions: temperature: 30°C; time: 72 hours; 150 rpm shaking; stirring regularly during static fermentation.

[0163] (3) After the fermentation is completed, the fermentation liquid and the solid residue are separated using a filter cloth; the fermentation liquid is subjected to low-temperature pasteurization (60-70° C., 30 minutes) and then evaporated under reduced pressure to concentrate the fermentation liquid to a solid content of 15%, thereby obtaining the Platycladus orientalis leaf fermentation filtrate.

[0164] The second aspect of this embodiment provides a composition, comprising the above-mentioned Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide, and the mass ratio of the Platycladus orientalis leaf fermentation filtrate, caviar and silicon dioxide is 1:1:8.

[0165] The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm, and the D50 distribution range is 9-14μm.

[0166] This embodiment provides a method for preparing the composition, and the steps are as follows:

[0167] S01 Dissolve 1 part of caviar powder in 8 parts of deionized water, use a stirrer, start at 2000r / s, stir for 0.5h, and set aside;

[0168] S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine;

[0169] S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state;

[0170] S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min;

[0171] S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water.

[0172] S06: Dry the obtained product to obtain a composite material.

[0173] The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

[0174] This embodiment provides a shampoo, and its preparation raw materials include, by weight:

[0175] 13 parts of a mixture of sodium laureth sulfate and water (mass ratio 7:3); 8 parts of a mixture of water, cocamidopropyl betaine, sodium chloride and sodium benzoate (mass ratio 65.5:29.5:4.5:0.5); 3 parts of a mixture of water, sodium lauroyl sarcosinate, sodium lauroyl glutamate and lauryl diethylenediaminoglycine (mass ratio 70:29.8:40.1:0.1); 3 parts of water, ethoxydiglycol, 1,2-pentanediol, PEG-40 hydrogenated castor oil, 1,2-hexanediol, ginseng (PANAX GINSENG) root extract mixture (mass ratio 83.5:10:2.5:2:1:1) 2 parts; polydimethylsiloxane, water, sodium laureth sulfate, sodium chloride mixture (mass ratio 70:20.6:8.4:1) 1.2 parts; cocamide MEA, methyl cocoate mixture (mass ratio 95:5) 1.1 parts; composition 5 parts; water, ammonium lauryl sulfate, hydrogenated castor oil, cocamide MEA, phenoxyethanol, disodium EDTA, sodium benzoate mixture (mass ratio 72.9:10:8:8:0.9:0.1:0.1 ) 1 part; sodium chloride 0.8 part; niacinamide 0.5 part; phenoxyethanol 0.4 part; taurine 0.35 part; sodium benzoate 0.3 part; guar hydroxypropyltrimethylammonium chloride, water, sodium chloride mixture (mass ratio is 85:10:5) 0.3 part; adenosine 0.3 part; allantoin 0.17 part; creatine 0.03 part; panthenol 0.2 part; piroctone olamine salt 0.15 part; citric acid 0.12 part; disodium EDTA 0.1 part; (daily use) flavor 0.5 part; ginsenoside 0.003 part; cyclodextrin 0.15 part; maltodextrin 0.15 part; water is added to 100 parts.

[0176] Performance Testing

[0177] Test 1: The shampoos prepared in Examples 1 to 7 were placed in thermostats at 48°C, 5°C, and 25°C, respectively. After the samples were restored to room temperature on the 30th day, the color appearance of the samples and whether the solutions had faded, stratified, or precipitated were observed.

[0178] The stability of hot and cold cycles was tested by a 2-day hot and cold cycle. The solution was placed in a -18°C constant temperature box for 1 day and in a 48°C constant temperature box for 1 day. The appearance of the solution was observed after returning to room temperature. A total of five cycles were monitored.

[0179] Table 1 Test results of test 1

[0180] Example 0℃ 25℃ 40℃ Hot and cold cycle experiment Example 1 No change No change No change No change Example 2 No change No change Slightly delaminated Slightly delaminated Example 3 No change No change No change No change Example 4 No change Layering Layering Layering Example 5 No change No change No change Layering Example 6 No change No change Layering Layering Example 7 No change No change No change No change

[0181] Test 2: Human Trial

[0182] 1. Materials and Methods

[0183] 1. Trial materials: Examples 1 to 7

[0184] 2. Subjects: A total of 30 people, including 10 males and 20 females, aged 30 to 60 years old, with an average age of 42.53±8.21 years old, who met the voluntary inclusion criteria.

[0185] 3. Trial method: Have the subjects use the test product directly according to the usage characteristics and methods indicated on the cosmetic product label, observe or follow up by phone once a week for the skin reactions of the subjects, and record the results according to the grading standards for adverse skin reactions in human trial tests in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The trial period shall be no less than 4 weeks.

[0186] Table 2 Test results of test 2

[0187]

[0188]

[0189] Test 3: Cosmetic efficacy test

[0190] Materials and methods

[0191] 1. Test product: Example 7

[0192] 2. Comparative product: Example 1 to Example 6

[0193] 3. Wash-out products: Same as the control products. Both the test group and the control group use the control products during the wash-out period.

[0194] 4. Instruments and Equipment

[0195] 4.1 Professional digital camera: The pixel is not less than 15 million. The parameters including aperture size, sensitivity (ISO) and focal length should remain consistent throughout the test.

[0196] 4.2 Image shooting bracket: It can fix the position of the subject's head so that the top of the head is perpendicular to the camera lens, and use a professional digital camera to take photos of all the hair with the top of the head as the center.

[0197] 4.3 Dermatoscope: LED light source, wavelength 450-750nm, color temperature 6500-8000K, illumination not less than 540lx, magnification ≥20 times, detection diameter not less than 1.0cm or area not less than 0.8cm 2 .

[0198] 4.4 Comb: The tooth density is moderate (tooth spacing 0.9-1.1mm), the tooth length is 2.0-3.0cm, and the comb length is not less than 10cm (excluding the comb handle). The comb of the same specification and material must be used throughout the test. After each use, it must be disinfected in accordance with the relevant requirements of the "Technical Specifications for Disinfection of Medical Institutions" (WS-T 367-2012).

[0199] 5. Subjects: A total of 60 subjects completed the test, aged 19 to 60 years old, including 24 males and 36 females, with an average age of 42.43±9.32 years old. They met the voluntary inclusion and exclusion criteria.

[0200] 6. Test method: The test shall be carried out in accordance with the specific requirements of the currently valid technical specifications and methods. Subjects who pass the initial screening are required to undergo a 2-week wash-out period, and will be screened again after the wash-out period. Qualified subjects selected for the formal trial are divided into the test group and the control group according to the stratified random method, and the test product and the control product are distributed respectively. Use the product correctly according to the product instructions. During the 12-week trial, subjects are required to keep a usage diary and strictly abide by the restrictions. Visits are conducted before product use (baseline value) and 12 weeks after use. The visits are for hair loss counts, assessment of local hair density, visual assessment of hair density, image capture, and records.

[0201] Table 3 Test results of test 3

[0202]

[0203] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fermentation filtrate of Platycladus orientalis leaves, characterized in that: The fermentation filtrate is prepared by liquid fermentation of Platycladus orientalis leaves; the fungi used are Bacillus and / or Aspergillus.

2. The fermentation filtrate of Platycladus orientalis leaves according to claim 1, characterized in that: The bacterial species used in the fermentation are Bacillus subtilis, Bacillus cereus and Aspergillus.

3. A caviar Platycladus orientalis leaf nano-encapsulated sustained-release composition, characterized in that: The invention comprises the fermentation filtrate of Platycladus orientalis leaves as claimed in claim 1 or 2, caviar and silicon dioxide.

4. The caviar Platycladus orientalis leaf nano-encapsulated sustained-release composition according to claim 3, characterized in that: The specific surface area of ​​the silicon dioxide is 208.02618 m 2 / g, pore volume is 0.88647cm 3 , the pore size is 19.04533nm.

5. The method for preparing a composition according to claim 3, characterized in that: Here are the steps: S01 Dissolve caviar powder in deionized water, use a stirrer, start stirring for 0.5h, and set aside; S02 placing silicon dioxide powder into the pelletizing chamber of a pelletizing machine; S03: Turn on the bottom induced draft fan to introduce air into the distributor at the bottom of the container, so that the silicon dioxide particles are fluidized and form a suspended jumping state; S04 turns on the high-pressure atomizing spray gun, and the spray gun sprays the caviar solution and the fermented filtrate of Platycladus orientalis leaves into the pelleting chamber of the pelleting machine at a flow rate of 15 ml / min; S05: Continue to add deionized water until the silica particles form 40-60 mesh round balls, then stop adding deionized water. S06: Dry the obtained product to obtain a composite material.

6. The method for preparing a composition according to claim 5, characterized in that: The silicon dioxide in the S02 is pre-treated, and the steps are as follows: the silicon dioxide is immersed in a 0.1wt% sodium hydroxide solution for 2h.

7. A shampoo, characterized in that: In parts by mass, the raw materials for its preparation include: A solvent, a composition as claimed in claim 3, a surfactant, a thickener, a preservative, a moisturizer, an antidandruff agent, a pH adjuster, a chelating agent, a fragrance, and a hair conditioner.

8. The shampoo according to claim 7, characterized in that The surfactant comprises sodium laureth sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, sodium lauroyl glutamate, lauryl diethylenediaminoglycine, and ammonium lauryl sulfate.

9. The shampoo according to claim 7, characterized in that The hair conditioning agent comprises ginseng root extract, adenosine, allantoin and creatine.

10. The fermented filtrate of Platycladus orientalis leaves according to claim 1, characterized in that Used in daily chemical field.

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