Composition and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition and application thereof. The invention relates to a composition, in particular to a composition with repairing and anti-wrinkle effects and application thereof. The invention provides a composition. The composition comprises a first active composition and a second active composition, the first active composition comprises sodium hyaluronate, phytosterol oleate and a polygonatum odoratum extract; and the second active composition comprises any one of the following two components: dipalmitoyl hydroxyproline or palmitoyl tripeptide-1. The applicant creatively discovers that by using the composition disclosed by the invention, through the synergistic effect of all the components, excellent repairing and anti-wrinkle effects can be formed.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and specifically, relates to a composition and application thereof; more specifically, relates to a composition with repairing and anti-wrinkle effects and application thereof. Background Art

[0002] Human skin is easily affected by the external environment, which can lead to dryness, peeling, cracks, etc., especially the lip skin, which has a thinner stratum corneum than other parts of the face, rich blood vessels, no sweat glands and sebaceous glands, and cannot moisturize itself. And as the lip skin ages, wrinkles, sagging, and dull color may appear, affecting the sense of youth. In addition, some ingredients in lip care products or lipsticks may cause allergic reactions, resulting in redness, swelling, itching or discomfort.

[0003] However, most of the lip products in the prior art are only considered from the perspective of color, but are not good in terms of repairing effect on the lips, and it is difficult to achieve the purpose of deep nourishment and repair.

[0004] Therefore, there is a need in the art for a product that has the effects of nourishing and repairing, can improve the health of the skin during daily lip care, and at the same time, can also solve problems such as wrinkles and sagging of the skin, reflecting a youthful look. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition with nourishing and repairing effects, which can also solve problems such as wrinkles and sagging of the skin.

[0006] In view of this, in a first aspect of the present invention, the present invention provides a composition comprising a first active composition and a second active composition, wherein:

[0007] The first active composition comprises:

[0008] Sodium hyaluronate, phytosteryl oleate, and polygonatum odoratum extract;

[0009] The second active composition includes any one of the following:

[0010] Dipalmitoyl hydroxyproline, or palmitoyl tripeptide-1.

[0011] The applicant creatively discovered that the composition of the present invention can achieve excellent repairing and anti-wrinkle effects through the synergistic effect of various components.

[0012] Furthermore, the mass ratio of the first active composition: the second active composition in the composition of the present invention may be (1-7):(7-1).

[0013] Preferably, the mass ratio of the first active composition: the second active composition in the composition of the present invention may be (1-5):(5-1).

[0014] Most preferably, the mass ratio of the first active composition: the second active composition in the composition of the present invention may be 5:3.

[0015] For example, per 100 g of the composition of the present invention, 12.5 g to 87.5 g of the first active composition and 12.5 g to 87.5 g of the second active composition may be contained. Preferably, per 100 g of the lip composition of the present invention, 62.5 g of the first active composition and 37.5 g of the second active composition may be contained.

[0016] By configuring and using the components of the composition of the present invention according to the above ratios, the repairing and anti-wrinkle effects of the composition can be further enhanced.

[0017] Furthermore, in the first active composition of the present invention, the mass ratio of sodium hyaluronate: phytosteryl oleate: polygonatum odoratum extract can be (1-5):(1-5):(1-5).

[0018] Preferably, in the first active composition of the present invention, the mass ratio of sodium hyaluronate: phytosteryl oleate: polygonatum odoratum extract can be (1-2):(1-2):(1-2).

[0019] Most preferably, in the first active composition of the present invention, the mass ratio of sodium hyaluronate: phytosteryl oleate: polygonatum odoratum extract can be 2:2:1.

[0020] In a second aspect, the present invention provides a use of the above composition in repairing and / or anti-wrinkle cosmetics and / or skin care products.

[0021] Furthermore, a use of the above composition for skin repair and / or anti-wrinkle, and / or lip repair and / or anti-wrinkle effects is provided.

[0022] Furthermore, the cosmetics and / or skin care products can be used on the face or on the lips.

[0023] Preferably, the cosmetics and / or skin care products may be cosmetics for lips, or skin care products for lips.

[0024] In a third aspect, the present invention provides a cosmetic and / or skin care product comprising the composition as described above.

[0025] Furthermore, the mass fraction of the composition in the cosmetics and / or skin care products is 0.01-5%, and can be 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5% in the cosmetics and / or skin care products. Preferably, the mass fraction of the composition in the cosmetics and / or skin care products is 1%.

[0026] For example, every 100 g of cosmetics and / or skin care products can include 0.01 g-5 g of the composition of the present invention, such as 0.01 g, 0.05 g, 0.1 g, 0.5 g, 1 g, 2 g, 3 g, 4 g, or 5 g of the composition of the present invention. Preferably, every 100 g of cosmetics and / or skin care products can include 1 g of the composition of the present invention.

[0027] Specifically, every 100 g of cosmetics may include 0.625 g of the first active composition and 0.375 g of the second active composition. More specifically, every 100 g of cosmetics may include 0.25 g of sodium hyaluronate, 0.25 g of phytosteryl oleate, 0.125 g of Polygonatum odoratum extract, and 0.375 g of dipalmitoyl hydroxyproline, or palmitoyl tripeptide-1.

[0028] Furthermore, the cosmetics and / or skin care products can be used on the face or on the lips.

[0029] Preferably, the cosmetics and / or skin care products may be cosmetics for lips, or skin care products for lips.

[0030] The cosmetics used on the lips can be lipstick, lip glaze, lipstick, lip gloss, lip gloss, lip cream, etc.

[0031] The dosage form that can be used as a cosmetic is a stick, dish, ointment, or the like.

[0032] In the present invention, in addition to the above-mentioned ingredients, the cosmetics may also contain any ingredients commonly used in cosmetics and pharmaceuticals. As the optional ingredients, the following ingredients may be mentioned, and one or more than one may be contained as long as the effects of the present invention are exerted.

[0033] In some specific embodiments, the cosmetic of the present invention further comprises one or more of an emollient, a filler, a colorant, a synthetic wax, an oil, a humectant, a preservative, a binder, a thickener, or an emulsifier.

[0034] The cosmetic of the present invention may contain any amount of water. As the water, water used in cosmetics, quasi-drugs, etc. can be used, for example, ion-exchanged water, distilled water, ultrapure water, tap water, etc. can be used.

[0035] In some specific embodiments, the filler of the present invention can be one or more of silica, polymethylsilsesquioxane, mica, muscovite, phlogopite, synthetic mica, red mica, or biotite.

[0036] Furthermore, the cosmetics used on the lips may be colorless lip cosmetics or colored lip cosmetics.

[0037] Furthermore, the lip cosmetic comprises a colorant.

[0038] In some specific embodiments, the colored lip product contains a colorant, such as CI77491, CI 15850, CI 77499, CI 77891, CI 77492, etc., and may also be titanium dioxide, iron red, red 7, iron yellow, iron black, etc. Preferably, it may be one or more of the aforementioned colorants.

[0039] In some specific embodiments, the synthetic wax of the present invention can be exemplified by microcrystalline wax, beeswax, candelilla wax, ozokerite, cotton wax, carnauba wax, bayberry wax, insect wax, spermaceti, montan wax, rice bran wax, lanolin, kapok wax, acetylated lanolin, lanolin oil, sugarcane wax, isopropyl lanolin, hexyl laurate, hydrogenated lanolin, jojoba wax, hard lanolin, shellac wax, etc.

[0040] In some specific embodiments, the moisturizing agent of the present invention can include polyethylene glycol, propylene glycol, glycerol, 1,3-butylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucin sulfate, cholesterol-12-hydroxystearate, sodium lactate, bile acid salts, pyrrolidone carboxylates, short-chain soluble collagen, diglycerol PO adduct, yarrow extract, fumitory extract, etc.

[0041] In some specific embodiments, the preservatives of the present invention can include parahydroxybenzoates such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, butyl parahydroxybenzoate, benzoic acid, salicylic acid, sorbic acid, para-chloromecresol, hexachlorophene, benzalkonium chloride, chlorhexidine, trichlorocarbanilide, photosensitizer, phenoxyethanol, etc.

[0042] In some specific embodiments, the thickener of the present invention can include gum arabic, carrageenan, carrageenan, tragacanth gum, carob bean gum, casein, dextrin, gelatin, sodium pectate, sodium alginate, methyl cellulose, ethyl cellulose, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, PVA, PVM, PVP, sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, dialkyl dimethyl ammonium cellulose sulfate, xanthan gum, magnesium aluminum silicate, bentonite, hectorite, propolis, silicic anhydride, etc.

[0043] In some specific embodiments, the emulsifier of the present invention can include nonionic surfactants, amphiphilic polymers, such as (acrylate / methacrylate stearyl alcohol polyether-20) copolymer, hydrophobically modified alkyl cellulose, hydrogenated lecithin, sorbitan laurate, sorbitan palmitate, sorbitan oleate, sorbitan stearate, sorbitan isostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan tristearate, sorbitan sesquiisostearate.

[0044] In a specific embodiment, the lip cosmetics further include candelilla wax, microcrystalline wax, ozokerite, isopentyl laurate, jojoba seed oil, squalane, caprylic / capric triglyceride, polyglyceryl-2 triisostearate, tocopherol, silica, iron red powder, red 7 powder, and titanium white powder.

[0045] In a fourth aspect, the present invention provides a synthetic carrier, on a part or all of the surface of which there is at least one layer of the lip composition of the present invention or the cosmetic of the present invention.

[0046] In a fifth aspect, the present invention provides a method for preparing a cosmetic, comprising the following steps:

[0047] Mixing the first active composition and the second active composition of the present invention and weighing them;

[0048] Mix and stir the weighed raw materials at 85-95°C until completely melted;

[0049] further adding one or more of synthetic wax, oil, emollient, filler, colorant, preservative, adhesive, thickener, stabilizer, or emulsifier, stirring evenly, and grinding;

[0050] The ground product is further stirred evenly to obtain a fluid, and finally the cosmetics are prepared.

[0051] In some specific embodiments, the steps include:

[0052] Heat the synthetic wax and / or grease at 85-90°C until completely melted;

[0053] further adding one or more of an emollient, a filler, a colorant, a preservative, a binder, a thickener, a stabilizer, or an emulsifier, stirring evenly, and grinding;

[0054] Mixing the first active composition and the second active composition of the present invention and weighing them;

[0055] The weighed raw materials are mixed and stirred at 85-95°C until completely melted and added to the completely melted synthetic wax and / or grease composition;

[0056] The product is further stirred evenly to obtain a fluid, and finally the cosmetics are prepared. DETAILED DESCRIPTION

[0057] The present invention will be described in detail below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments and examples are used to illustrate the present invention, rather than to limit the present invention.

[0058] The term "sodium hyaluronate" is a polysaccharide widely present in the human body. It is a disaccharide unit glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine, also known as hyaluronic acid. It has the effects of promoting wound healing, auxiliary treatment, moisturizing, and anti-wrinkle.

[0059] The term "phytosteryl oleate" refers to a lipid molecule with a structure similar to phytosterols in cholesterol. It is usually extracted from legumes (such as soybeans), cereals (such as wheat germ), vegetable oils (such as sunflower oil) or nut oils (such as almond oil). In cosmetics, it has moisturizing, barrier strengthening, reduction of transepidermal water loss, anti-inflammatory and soothing effects, protection against environmental stressors, enhancement of skin elasticity, anti-aging properties, and soothing of dry skin.

[0060] The term "dipalmitoyl hydroxyproline" is a derivative of an amino acid, also known as dipalmitoyl hydroxyproline. In cosmetics, dipalmitoyl hydroxyproline has good anti-aging and skin firming effects, is more active than vitamin C, can also increase the moisture content in the skin, and has a good moisturizing effect. At the same time, as a fat-soluble derivative of hydroxyproline, it is easier to penetrate into the dermis and promote the synthesis of collagen.

[0061] The term "palmitoyl tripeptide-1" is a peptide molecule composed of three amino acids, and its amino acid sequence is Pal-Gly-His-Lys-OH. It can effectively prevent and reduce wrinkles, making the skin younger, smoother and more elastic. Studies have shown that its activity in promoting the synthesis of collagen and glycosaminoglycans is comparable to that of vitamin A, but avoids its irritation and side effects.

[0062] The term "polygonatum odoratum extract" is POLYGONATUM ODORATUM, which is obtained by extracting the rhizome of polygonatum odoratum and is composed of a variety of compounds, including flavonoids, polysaccharides, organic acids and amino acids. It has extremely low toxicity and has antioxidant, anti-inflammatory, anti-tumor, anti-aging and antibacterial activities to a certain extent. It can improve the condition of dry and rough skin, make it soft and smooth, and thus play a role in beauty and skin care.

[0063] Example 1: Formula for preparing cosmetics using the composition of the present invention

[0064] The formula of the lip composition of the present invention for preparing cosmetics is shown in Table 1. Table 1 lists the components of various lip compositions used in the present invention for preparing cosmetics, and the values ​​in the table are all parts by weight.

[0065] Table 1. Formula for preparing cosmetics based on the composition of the present invention

[0066]

[0067] The preparation methods of the lip products in Comparative Examples 1-6 are the same as those in Examples 1 and 2, and only some components in the formulas used are different. Please see Table 2 below for specific differences. The present invention designs a variety of comparisons, including formulas that do not include the second active composition, and formulas in which the second active composition has two kinds.

[0068] Table 2. Differences between the examples and comparative examples

[0069] Serial number Notes Comparative Example 1 The difference from Examples 1 and 2 is that the product contains only sodium hyaluronate Comparative Example 2 The difference from Examples 1 and 2 is that the product contains only phytosteryl oleate Comparative Example 3 The difference from Examples 1 and 2 is that the product contains only palmitoyl tripeptide-1 Comparative Example 4 The difference from Examples 1 and 2 is that the product does not contain Polygonatum odoratum extract Comparative Example 5 The difference from Examples 1 and 2 is that the product does not contain the second active composition Comparative Example 6 The only difference from Examples 1 and 2 is that the product includes two second active compositions

[0070] Example 2: Preparation of cosmetics using the composition of the present invention

[0071] The cosmetics were prepared according to the formula described in Table 2'. Specifically, the waxes (candelilla wax, microcrystalline wax, ozokerite) and oily substances (isoamyl laurate, jojoba seed oil, squalane, caprylic / capric triglyceride, polyglyceryl-2 triisostearate) in each formula in Table 1 were heated to 85-90°C until completely melted, and then color powder and silica were added and stirred evenly, and then ground using a three-roll grinder. After the grinding process was repeated three times, the pre-prepared composition of the present invention was added and heated and stirred evenly at 85-95°C to obtain a fluid. Finally, the material temperature was 85-90°C, and the lipstick was obtained by filling, cooling, and demolding.

[0072] Table 2'

[0073] Raw material name Formula content (%) Candelilla wax 8 Microcrystalline wax 8 Ozokerite 5 Isoamyl laurate 19 Jojoba Seed Oil 15 Squalane 18 Caprylic / capric triglyceride 10 Polyglyceryl-2 Triisostearate 2 Tocopherol 1 Silica 9 Compositions of Examples or Comparative Examples of the Invention 1 Iron red powder 2 Red 7 color powder 0.3 Titanium white powder 1.7

[0074] Example 3: Testing of the repairing effect of the lip composition of the present invention

[0075] Repair effect testing principle:

[0076] The repair efficacy of the formula was tested using the zebrafish repair efficacy test method. The principle is to cut the tail fin perpendicular to the trunk with a scalpel, and use the quantitative results of the zebrafish regenerated tail fin area to evaluate the repair efficacy of the sample. The regeneration of the zebrafish tail fin is divided into three processes: wound healing, bud formation, and regeneration outcome, with bud formation being the core. There are multiple sources of cells for fin regeneration, including epidermal cells, fibroblasts, and osteoblasts, all of which promote the regeneration of the tail fin, and these cells are highly lineage-restricted. The regeneration of the zebrafish tail fin is similar to the repair mechanism of human skin, bones, blood vessels, etc. The zebrafish tail fin has become an important model for studying the tissue regeneration process due to its simple structure, easy surgical operation, no impact on survival after surgery, and easy observation.

[0077] Repair efficacy testing steps and result analysis:

[0078] The tail fin of zebrafish was removed with a scalpel to establish a zebrafish tail fin injury model, and the model zebrafish were randomly assigned to a 6-well plate, with 15 tails per well. The sample was given in water, and a normal control group and a model control group were set up at the same time, with a capacity of 3mL per well. Incubate in the dark for 48 hours at 28°C. Ten zebrafish were randomly selected from each experimental group and placed under a dissecting microscope to take pictures. The data were analyzed and collected using advanced image processing software, and the zebrafish tail fin area (A) was analyzed. The repair efficacy of the sample was calculated according to the formula to determine whether it had a repair efficacy. Statistical analysis p<0.05 was determined to be significantly different. The specific results are shown in Table 3. Under the same experimental conditions, the formulas of Examples 1 and 2 of the present invention and Comparative Example 6 have particularly significant repair effects, indicating that the effects of these three formulas are better (the detection concentration is 0.01% of the sample loading concentration of the composition of the present invention on zebrafish, and the solvent is ethanol).

[0079] Table 3

[0080] Formula Name Test items Detection concentration (%) effect(%) p-value Test results Comparative Example 1 Repair effect 0.01 4 >0.05 Not significant Comparative Example 2 Repair effect 0.01 7 >0.05 Not significant Comparative Example 3 Repair effect 0.01 8 ＜0.05 Significant Comparative Example 4 Repair effect 0.01 6 >0.05 Not significant Comparative Example 5 Repair effect 0.01 10 ＜0.05 Significant Example 1 Repair effect 0.01 16 ＜0.001 Particularly significant Example 2 Repair effect 0.01 17 ＜0.001 Particularly significant Comparative Example 6 Repair effect 0.01 13 ＜0.01 Particularly significant

[0081] Example 4: Anti-wrinkle efficacy test of the lip composition of the present invention

[0082] Anti-wrinkle efficacy testing principle:

[0083] The anti-wrinkle efficacy of the formula was tested using the zebrafish anti-wrinkle efficacy test method. Skin growth, repair, nutrition, elasticity and tension are all related to collagen. Its loss will reduce the smoothness of the skin and produce wrinkles. In tetrapods, type I collagen is a trimer, mainly composed of two α1 chains and one α2 chain, encoded by col1a1a and col1a2 genes, respectively, and performs collagen-related biological functions in connective tissue and bone. There are three type I collagen genes in zebrafish, col1a1a, col1a1b, and col1a2 encoding α1(I), α2(I) and α3(I) chains, respectively. Therefore, by detecting the relative expression of col1a1a or (and) col1a1b or (and) col1a2 genes, it can be indicated whether the sample has anti-wrinkle efficacy.

[0084] Anti-wrinkle efficacy testing steps and result analysis:

[0085] Zebrafish were randomly selected in a 6-well plate, with 30 fish in each well. The sample was given in water, and a normal control group was set up at the same time, with a capacity of 3 mL per well. Three biological replicates were performed. Incubate in the dark at 28°C for 24 hours. Total RNA of zebrafish in each experimental group was extracted, cDNA was synthesized, and q-PCR was used to detect the gene expression of β-actin and the target gene. β-actin was used as an internal reference for gene expression to calculate the relative RNA expression of the target gene. The specific results are shown in Table 4. Under the same experimental conditions, the anti-wrinkle effects of Examples 1 and 2 of the present invention are particularly significant, indicating that the effects of these two formulas are better. At the same time, it is also proved that the composition of the present invention can achieve both repair and anti-wrinkle effects through synergistic effects. On the contrary, when more ingredients are used in the second active composition, the effect is far less than that of using only one ingredient, indicating that the composition of the present invention works through specific synergistic effects, and it is not possible to achieve both repair and anti-wrinkle effects by randomly finding ingredients.

[0086] Table 4

[0087]

Claims

1. A composition comprising: A first active composition and a second active composition, wherein The first active composition comprises: Sodium hyaluronate, phytosteryl oleate, and polygonatum odoratum extract; The second active composition includes any one of the following: Dipalmitoyl hydroxyproline, or palmitoyl tripeptide-1.

2. The composition according to claim 1, characterized in that The mass ratio of the first active composition to the second active composition in the composition is (1-7):(7-1).

3. The composition according to claim 2, characterized in that The mass ratio of the first active composition to the second active composition in the composition is 5:

3.

4. The composition according to claim 1, characterized in that The mass ratio of sodium hyaluronate: phytosteryl oleate: polygonatum odoratum extract in the first active composition is (1-5):(1-5):(1-5).

5. The composition according to claim 4, characterized in that The mass ratio of sodium hyaluronate: phytosteryl oleate: polygonatum odoratum extract in the first active composition is 2:2:

1.

6. A cosmetic and / or skin care product comprising the composition according to any one of claims 1 to 5.

7. The cosmetic and / or skin care product according to claim 6, characterized in that: The mass fraction of the composition in cosmetics is 0.01% to 5%.

8. The cosmetic and / or skin care product according to claim 6 or 7, characterized in that: The cosmetics are cosmetics and / or skin care products for lips.

9. Use of the composition according to any one of claims 1 to 5 for skin repair and / or anti-wrinkle, and / or lip repair and / or anti-wrinkle effects.

10. A method for preparing a cosmetic, comprising: Mixing and weighing the composition according to any one of claims 1 to 5; Mix and stir the weighed raw materials at 85-95°C until they are completely melted; further adding one or more of synthetic wax, oil, emollient, filler, colorant, moisturizer, preservative, adhesive, thickener, or emulsifier, stirring evenly, and grinding; and The ground product is further stirred evenly to obtain a fluid, and finally the cosmetics are prepared.

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