Magnetic separation cleaning method, magnetic cleaning device and immunity analyzer

Abstract

The invention belongs to the technical field of medical instruments, and relates to a magnetic separation cleaning method, a magnetic cleaning device and an immunity analyzer, and the method comprises the following steps: transferring a reaction cup among a liquid injection station, an adsorption station and a liquid suction station; cleaning liquid is injected into the reaction cup at the liquid injection station; magnetic particle conjugates in the reaction cups are adsorbed to the inner walls of the reaction cups at the adsorption station, and waste liquid is extracted from the reaction cups injected with the cleaning liquid at the liquid suction station. After the steps are adopted, the magnetic separation cleaning device has the beneficial effects that by reasonably planning the number of the liquid injection stations and the number of the liquid suction stations and matching of the cleaning orders of magnetic separation cleaning, the structure of the magnetic separation cleaning device is effectively simplified, the size of the device is reduced, the manufacturing cost is reduced, and the cleaning efficiency of the magnetic separation cleaning device is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of automated analysis, and in particular relates to a magnetic separation and cleaning method, a magnetic cleaning device and an immunoassay analyzer. Background Art

[0002] Luminescent immunoassay is based on the immunological reaction of antigen-antibody binding. It uses enzymes, luminescent agents and other substances to label antigens and antibodies. After a luminescent reaction, the light signal is linked to the analyte concentration, etc., to analyze the analyte content in the sample.

[0003] Magnetic separation technology is to separate the magnetic particle complex from the reaction liquid by solid-liquid phase separation. In order to ensure the effect of magnetic separation, the reaction liquid needs to be fully mixed during the separation process, and the liquid needs to be repeatedly injected and aspirated to remove impurities, so as to obtain accurate test results in subsequent tests.

[0004] Specifically, in the magnetic separation and cleaning process, the target analyte in the sample is first captured and bound using magnetic particles (usually the main component of the magnetic particle reagent component) as a solid phase carrier, and then the magnetic particles directly or indirectly bound to the target analyte are adsorbed on the inner wall of the reaction cup through the magnetic field. After multiple injections of cleaning liquid and extraction of waste liquid, the free markers and other interfering impurities not bound to the magnetic particles are finally removed, so that the antigen-antibody complex (i.e., magnetic particle complex) connected to the magnetic particles can be detected for signal.

[0005] For traditional cleaning methods, the number of liquid injection stations is usually the same as the number of liquid suction stations. There are also cases where the number of liquid suction stations is more than the number of liquid injection stations, and the number of cleaning stages is equal to the number of liquid suction stations (the number of cleaning times corresponds to the number of stations). These cleaning methods make the device structure of the cleaning method complex and bulky. Therefore, it is necessary to make improvements. Summary of the invention

[0006] In order to solve the above problems in the prior art, the present invention provides a magnetic separation cleaning method, a magnetic cleaning device and an immunoassay analyzer, in order to improve the cleaning efficiency, simplify the structure of the magnetic separation cleaning device and reduce the size of the magnetic separation cleaning device.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] As one aspect of the present invention, a magnetic separation cleaning method is proposed, which comprises the following steps:

[0009] Transferring reaction cups between the injection station, adsorption station and aspiration station;

[0010] Injecting a cleaning solution into the reaction cup at the liquid injection station;

[0011] Adsorbing the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup at the adsorption station, and extracting waste liquid from the reaction cup into which the cleaning liquid has been injected at the liquid aspiration station;

[0012] The number of liquid injection stations is the same as the number of liquid aspiration stations, and at least one liquid injection station and liquid aspiration station are set; completing one liquid injection, one adsorption, and one liquid aspiration is called one-stage cleaning, and magnetic separation cleaning is 2-6-stage cleaning;

[0013] The number of injection stations is less than or equal to the number of cleaning stages required to complete a complete magnetic separation cleaning, and the number of cleaning stages required to complete a complete magnetic separation cleaning is at least 2 stages; to complete a complete magnetic separation cleaning, one injection station injects cleaning liquid into at least two reaction cups in succession, and one aspiration station extracts waste liquid into at least two reaction cups in succession.

[0014] Optionally, a plurality of cup grabbing stations are provided, and the manipulator grabs and places the reaction cups at different cup grabbing stations; the plurality of cup grabbing stations are located between the liquid injection station and the liquid aspiration station.

[0015] As one aspect of the present invention, a magnetic separation cleaning device for implementing the magnetic separation cleaning method as described above is proposed, comprising:

[0016] The carrying mechanism comprises a turntable for carrying the reaction cup and being intermittently rotatable, wherein the turntable is evenly provided with a plurality of carrying positions for carrying the reaction cup along its circumference, wherein the carrying positions can take the cup grabbing position as the initial position and go back to the cup grabbing position after at least one stage of cleaning;

[0017] A liquid injection mechanism, used for injecting cleaning liquid into the reaction cup;

[0018] A mixing mechanism for shaking the reaction cup;

[0019] A magnetic adsorption mechanism is used to adsorb the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup;

[0020] A liquid suction mechanism, used to extract waste liquid from a reaction cup that has been injected with cleaning liquid;

[0021] The number of the injection mechanisms is equal to the number of the suction mechanisms, and the turntable carries the reaction cup and intermittently rotates between the injection mechanism and the magnetic suction mechanism. When the rotation stops, the magnetic adsorption mechanism adsorbs the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup.

[0022] Optionally, a cleaning liquid injection component of a liquid injection mechanism and a liquid absorbing component of a liquid absorbing mechanism form a cleaning assembly;

[0023] The number of cleaning components is less than or equal to the number of cleaning stages of magnetic separation cleaning, and the required number of cleaning stages is achieved by carrying the reaction cup through the cleaning liquid injection part and the liquid suction part for cyclic cleaning;

[0024] The product of the number of gripping cup stations and the number of cleaning components is equal to the number of cleaning stages required to complete a complete magnetic separation cleaning.

[0025] Optionally, the cleaning method of the magnetic separation cleaning device comprises the following steps:

[0026] S801, the turntable intermittently rotates to transfer the reaction cup at the first cup grabbing station to the liquid injection station;

[0027] S802, injecting cleaning solution into the reaction cup at the liquid injection station;

[0028] S803, the turntable rotates the reaction cup to the adsorption station, adsorbs the magnetic particle binding material suspended in the reaction cup to the inner wall of the supporting reaction cup at the adsorption station, and extracts waste liquid from the reaction cup into which the cleaning liquid has been injected at the liquid aspiration station;

[0029] S804, the turntable rotates intermittently to transfer the reaction cup to the first cup grabbing station, and the robot puts the reaction cup of the next cycle to the second cup grabbing station;

[0030] S805, the turntable transfers the first reaction cup to the liquid injection station for second-stage cleaning, and the cycle continues to complete the cleaning stages of magnetic separation cleaning required for the reaction cup.

[0031] Optionally, the mixing mechanism is arranged above the turntable or the mixing mechanism is arranged below the turntable;

[0032] The mixing mechanism is used for mixing the reaction cups on the turntable; the mixing mechanism comprises a base, a motor fixing seat is fixedly connected to the base, and a first power unit is connected to the motor fixing seat; the movable end of the first power unit is connected to a first rotating shaft through a coupling to transmit power; the first rotating shaft is rotatably connected to a mixing seat plate arranged on the base, and the first rotating shaft can rotate on the mixing bottom plate; the other end of the first rotating shaft extends to the outside of the mixing bottom plate, and the other end of the first rotating shaft is fixedly connected to an eccentric wheel;

[0033] There is a distance between the center of the eccentric wheel and the center of the first rotating shaft; a circular slide groove is formed on the eccentric wheel, a cam follower is slidably connected in the circular slide groove, and the cam follower slides in the circular slide groove; a sliding connecting plate is movably connected to the outer end of the cam follower, and the other end of the sliding connecting plate is connected to a sliding plate;

[0034] The mixing seat plate is fixedly connected to a first slide rail arranged longitudinally, the first slide rail is slidably connected to a second slide block, and the slide plate is fixed to the first slide block;

[0035] The sliding plate is connected to a mixing assembly, which includes a mixing motor fixing plate connected to the sliding plate, and the mixing motor is connected to the mixing motor fixing plate; the movable end of the mixing motor is connected to a mixing accommodating portion that matches the reaction cup; the center of the mixing accommodating portion does not coincide with the center of the movable end of the mixing motor.

[0036] Optionally, a liquid injection mechanism is provided above the mixing mechanism; the magnetic adsorption mechanism is fixed on the mixing mechanism; the magnetic adsorption mechanism is used to adsorb the magnetic beads in the reaction cup on the turntable to prevent them from being sucked away by the liquid aspiration mechanism;

[0037] The magnetic adsorption mechanism includes a magnet seat, which is detachably connected between the top of the mixing seat plate and the top of the motor fixing seat; a magnet is provided on the magnet seat, and the magnet is carried on an annular magnet seat, and an annular magnet cover is connected to the magnet seat to limit and fix the magnet.

[0038] Optionally, the bearing mechanism is connected to the mixing mechanism; the bearing mechanism is used to position and support the reaction cup, and the bearing mechanism includes a motor seat, a second power unit is connected to the motor seat, and a movable end of the second power unit is connected to a second rotating shaft.

[0039] A rotating bearing is connected to the second rotating shaft, and the outer ring of the rotating bearing is connected to the annular magnet seat of the mixing mechanism. The motor seat is connected between the mixing seat plate and the motor fixing seat; the other end of the second rotating shaft is connected to a turntable, and the turntable is provided with a plurality of bearing positions for carrying reaction cups; the turntable is evenly provided with a plurality of bearing positions along its circumference for carrying reaction cups.

[0040] Optionally, the injection mechanism is fixed on the magnetic adsorption mechanism; the injection mechanism is used to inject cleaning liquid into the reaction cup, the injection mechanism includes an injection base, and the injection connecting seat is detachably connected to the magnet seat of the magnetic adsorption mechanism; the injection connecting seat is connected to a column; the column is connected to the injection base, and the injection base is connected to a cleaning liquid injection piece; the cleaning liquid injection piece is connected to the injection base through a first injection piece positioning seat, and the first injection piece positioning seat is connected to an injection needle pressure plate, which presses and limits the cleaning liquid injection piece; the first injection piece positioning seat is provided with a first through hole for cooperation with the cleaning liquid injection piece, which is used for the passage of the cleaning liquid injection piece.

[0041] As an option, the magnetic liquid suction mechanism is arranged on the mixing mechanism; the liquid suction mechanism is used to suck the cleaning liquid in the reaction cup; the magnetic liquid suction mechanism includes a liquid suction vertical rod, and the liquid suction vertical rod is connected to the base; the liquid suction vertical rod is connected to a screw motor, and the screw nut of the screw motor is connected to a screw seat; the screw seat is connected to a longitudinally arranged second slide rail, and the second slide rail is slidably connected to a second slider, and the second slider is connected to a slider seat; the slider seat is detachably connected to the motor fixing seat; the upper part of the second slide rail is connected to a liquid suction needle fixing rod, the liquid suction needle fixing rod is connected to a liquid suction needle connecting seat, the liquid suction needle connecting seat is connected to a liquid suction piece, and the liquid suction needle connecting seat is connected to a liquid suction needle pressure block, and the liquid suction needle pressure block presses and limits the liquid suction piece;

[0042] It also includes a liquid-absorbing needle positioning seat, which is connected to the liquid-injection base. The bottom end of the liquid-absorbing component movably passes through the through hole of the liquid-absorbing needle positioning seat to cooperate with the corresponding reaction cup.

[0043] Optionally, it also includes a substrate injection station, on which a substrate injection mechanism is provided, and a substrate injection mechanism is provided at a carrying position of a reaction cup behind the liquid aspiration mechanism, and a mixing component is provided at the corresponding station, and there are two mixing components, one mixing component is used for mixing after the cleaning liquid is injected, and the other mixing component is used for mixing after the substrate is injected;

[0044] The substrate injection mechanism includes an A-liquid injection needle, which is connected to the injection base through a second injection piece positioning seat, and the A-liquid injection needle and the cleaning liquid injection piece are arranged at an interval; the second injection piece positioning seat is connected to an injection needle pressure plate, and the injection needle pressure plate presses and limits the A-liquid injection needle; the second injection piece positioning seat is provided with a second through hole for the A-liquid injection needle to cooperate with, which is used for the A-liquid injection needle to pass through.

[0045] Optionally, the injection station, the pipetting station, the substrate injection station and the cup grabbing station are arranged at intervals along the same circumference of the turntable, and the turntable drives the reaction cup to perform circular motion among the injection station, the pipetting station and the substrate injection station.

[0046] As a third aspect of the present invention, an immunoassay analyzer is provided, which includes the magnetic cleaning device as described above.

[0047] The beneficial effects of the magnetic separation cleaning method, magnetic cleaning device and immunoassay analyzer of the present invention are specifically embodied in that: a plurality of cup grabbing stations are provided on the turntable; the product of the liquid injection station and the cup grabbing station is equal to a complete cleaning stage; and the plurality of cup grabbing stations are rationally utilized for cyclic cleaning to achieve the required cleaning stages. By rationally planning the number of liquid injection stations and liquid aspiration stations and the coordination of the cleaning stages of magnetic separation cleaning, the structure of the magnetic separation cleaning device is effectively simplified, the volume of the device is reduced, the manufacturing cost is reduced, and the cleaning efficiency of the magnetic separation cleaning device is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] The drawings in the specification, which constitute a part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0049] Figure 1 It is a structural schematic diagram of the magnetic cleaning device of the present invention;

[0050] Figure 2 It is a rear view of the structure of the magnetic cleaning device of the present invention;

[0051] Figure 3 This is a diagram showing the placement state of the reaction cups in the first circle of the magnetic cleaning device of the present invention;

[0052] Figure 4 It is a structural schematic diagram of the mixing mechanism of the present invention;

[0053] Figure 5 It is a structural schematic diagram of the magnetic adsorption mechanism of the present invention;

[0054] Figure 6 It is a structural schematic diagram of the bearing mechanism of the present invention;

[0055] Figure 7 It is a structural schematic diagram of the liquid injection mechanism of the present invention;

[0056] Figure 8 It is a structural schematic diagram of the liquid aspiration mechanism of the present invention;

[0057] Fig. 9 It is a schematic diagram of the process of the magnetic cleaning device of the present invention. DETAILED DESCRIPTION

[0058] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the specific embodiments of the present invention and the corresponding drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0059] A magnetic separation and cleaning method according to an embodiment of the present application comprises the following steps:

[0060] A plurality of cup grabbing stations are provided, and the robot arm of the analyzer grabs and places the reaction cup at different cup grabbing stations in each cycle;

[0061] The reaction cup is transferred between the liquid injection station, the adsorption station and the liquid aspiration station; a plurality of cup grabbing stations are located between the liquid injection station and the liquid aspiration station;

[0062] Injecting a cleaning solution into the reaction cup at the liquid injection station;

[0063] Adsorbing the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup at the adsorption station, and extracting waste liquid from the reaction cup into which the cleaning liquid has been injected at the liquid aspiration station;

[0064] Among them, the number of injection stations is the same as the number of aspiration stations, and at least one injection station and aspiration station are set; completing one injection, one adsorption, and one aspiration is called one-stage cleaning, and magnetic separation cleaning is 2-6-stage cleaning; the above design, through reasonable planning of the number of injection stations and aspiration stations and the coordination of the cleaning stages of magnetic separation cleaning, effectively simplifies the structure of the magnetic separation cleaning device, reduces the volume of the device and reduces the manufacturing cost, and improves the cleaning efficiency of the magnetic separation cleaning device.

[0065] A magnetic separation cleaning device according to an embodiment of the present application, such as Figure 1-Figure 8 As shown, the magnetic separation and cleaning device is used to clean the magnetic particle binding material inside the reaction cup 70, and the magnetic separation and cleaning device includes a carrying mechanism 40, a liquid injection mechanism 50, a liquid suction mechanism 60, a mixing mechanism 20, and a magnetic adsorption mechanism 30;

[0066] The carrying mechanism 40 includes a turntable 401 for carrying the reaction cup 70 and can rotate intermittently. The turntable 401 is evenly arranged along its circumference with a plurality of carrying positions for carrying the reaction cup. The carrying positions can take the cup grabbing position as the initial position and go back to the cup grabbing position after at least one stage of cleaning.

[0067] The liquid injection mechanism 50 is used to inject the cleaning liquid into the reaction cup 70;

[0068] A mixing mechanism 20, used for shaking the reaction cup 70;

[0069] The magnetic adsorption mechanism 30 is used to adsorb the magnetic particle conjugate in the reaction cup 70 onto the inner wall of the reaction cup 70;

[0070] The liquid suction mechanism 60 is used to extract waste liquid from the reaction cup 70 into which the cleaning liquid has been injected;

[0071] Among them, the number of the injection mechanisms 50 is equal to the number of the suction mechanisms 60, and the turntable 401 carries the reaction cup 70 and intermittently rotates between the injection mechanism 50 and the magnetic suction mechanism 60. When the rotation stops, the magnetic adsorption mechanism 30 adsorbs the magnetic particle complex in the reaction cup 70 on the inner wall of the reaction cup 70.

[0072] In this embodiment, a cleaning liquid injection part 507 of a liquid injection mechanism 50 and a liquid absorbing part 601 of a liquid absorbing mechanism 60 form a cleaning assembly;

[0073] The number of cleaning components is less than or equal to the cleaning stages of magnetic separation cleaning, and the required cleaning stages are achieved by carrying the reaction cup 70 through the cleaning liquid injection part 507 and the liquid absorption part 601 for cyclic cleaning.

[0074] Take the reaction cup 70 for example to complete one stage of cleaning. Figure 1-Figure 3 and Fig. 9 As shown, the cleaning method of the magnetic separation cleaning device comprises the following steps:

[0075] S801, the turntable 401 intermittently rotates to transfer the cuvette 70 at the first cup grabbing station to the liquid injection station;

[0076] S802, injecting cleaning liquid into the reaction cup 70 at the liquid injection station;

[0077] S803, the turntable 401 rotates the reaction cup 70 to the adsorption station, adsorbs the magnetic particle binding material suspended in the reaction cup 70 to the inner wall of the supporting reaction cup 70 at the adsorption station, and extracts the waste liquid from the reaction cup 70 into which the cleaning liquid has been injected at the liquid aspiration station;

[0078] S804, the turntable 401 intermittently rotates to transfer the reaction cup 70 to the first cup grabbing station, and the robot puts the reaction cup of the next cycle to the second cup grabbing station;

[0079] S805, the turntable 401 turns the first reaction cup 70 to the liquid injection station for second-stage cleaning;

[0080] Among them, the number of injection stations is equal to the number of aspiration stations, the number of injection stations is less than or equal to the number of cleaning stages required to complete a complete magnetic separation cleaning, and the number of cleaning stages required to complete a complete magnetic separation cleaning is at least 2 stages of cleaning; to complete a complete magnetic separation cleaning, one injection station injects cleaning liquid into at least two reaction cups 70 in succession, and one aspiration station extracts waste liquid from at least two reaction cups 70 in succession.

[0081] The product of the number of cup gripping stations and the number of cleaning components is equal to the number of cleaning stages required to complete a complete magnetic separation cleaning.

[0082] For example, there can be three cup grabbing stations, marked as the first cup grabbing station, the second cup grabbing station, and the third cup grabbing station, and there can be one or two cleaning components. The three cycles can respectively realize the three-stage cleaning and the six-stage cleaning;

[0083] There can also be two cup grabbing stations, marked as the first cup grabbing station and the second cup grabbing station respectively. There can be one or two cleaning components, and two cycles can respectively achieve two-stage cleaning and four-stage cleaning.

[0084] For a single cuvette 70, in order to ensure a good cleaning effect, a complete cleaning process includes at least three stages of cleaning, that is, three times of injecting cleaning liquid and extracting waste liquid. The following is an explanation of the situation of a set of cleaning components performing three stages of cleaning:

[0085] Step 1: The robot moves the first cuvette 70 to the first cup grabbing station of the turntable 401, and the turntable 401 rotates it to the injection station. The cleaning liquid injection part 507 of the injection mechanism 50 injects cleaning liquid into the cuvette 70, and the mixing mechanism 20 moves upward to drive the cuvette 70 to rotate, and the suspended liquid in the cuvette 70 produces eccentric oscillation to achieve a mixing effect; after the mixing is completed, the mixing mechanism 20 moves downward, and the cuvette 70 is separated from the mixing mechanism 10, so that the turntable 401 rotates to drive the cuvette 70 to leave the injection station; at this point, the reaction cup 70 completes the first stage of liquid injection.

[0086] Step 2: The turntable 401 transfers the reaction cup 70 injected with the cleaning liquid from the liquid injection station to the adsorption station. At the adsorption station, the magnetic particle conjugate suspended in the reaction cup 70 is adsorbed onto the inner wall of the supporting reaction cup 70, and the waste liquid is extracted from the reaction cup 70 injected with the cleaning liquid at the liquid aspiration station. The reaction cup 70 completes the first stage of liquid aspiration. Then, the turntable 401 rotates intermittently to transfer the reaction cup 70 to the liquid injection station for the second stage of liquid injection. This cycle is repeated to complete the third stage of cleaning.

[0087] Specifically, in one embodiment, at least one mixing mechanism 20 is provided for shaking the reaction cup 70 ; the mixing mechanism 20 is provided above the turntable 401 or the mixing mechanism 20 is provided below the turntable 401 .

[0088] In this embodiment, if Figure 4As shown, the mixing mechanism 20 is mainly used for mixing the reaction cup 70 located on the turntable 401, and the mixing mechanism 20 can rotate up and down relative to the turntable 401; the mixing mechanism 20 includes a base 215, and a motor fixing seat 217 is fixedly connected to the base 215, and a first power unit 204 is connected to the motor fixing seat 217; the first power unit 204 is a motor, and its structure is the prior art and is not repeated here; the movable end of the first power unit 204 is connected to a first rotating shaft 208 through a coupling 220 to transmit power; the first rotating shaft 208 is rotatably connected to a mixing seat plate 216 provided on the base 215, and the first rotating shaft 208 can rotate on the mixing bottom plate 216; the other end of the first rotating shaft 208 extends to the outside of the mixing bottom plate 216, and the other end of the first rotating shaft 208 is fixedly connected to an eccentric wheel 201;

[0089] There is a distance between the center of the eccentric wheel 201 and the center of the first rotating shaft 208; a circular slide groove 2011 is formed on the eccentric wheel 201, and a cam follower 209 is slidably connected in the circular slide groove 2011, and the cam follower 209 slides in the circular slide groove 2011; the structure of the cam follower 209 is the prior art and will not be described here; the outer end of the cam follower 209 is movably connected to a sliding connecting plate 202, and the other end of the sliding connecting plate 202 is connected to a sliding plate 210;

[0090] The mixing seat plate 216 is fixedly connected to a longitudinally arranged first slide rail 207, and the first slide rail 207 is slidably connected to a second slider 2071. The sliding plate 210 is fixed on the first slider 2071. The first power unit 204 works to drive the eccentric wheel 201 to rotate, and then drives the sliding connecting plate 202 connected to the cam follower 209 to move up and down relative to the base 215, thereby realizing the up and down movement of the sliding plate 210 relative to the base 215.

[0091] Specifically, the sliding plate 210 is connected to a mixing component, and the mixing component includes a mixing motor fixing plate 211 connected to the sliding plate 210, and the mixing motor fixing plate 211 is connected to a mixing motor 205; the movable end of the mixing motor 205 is connected to a mixing accommodating portion 203 that cooperates with the reaction cup 70, and as an example, the mixing accommodating portion 203 is a recessed portion; the center of the mixing accommodating portion 203 does not coincide with the center of the movable end of the mixing motor 205, that is, there is a preset distance between the center of the mixing accommodating portion 203 and the center of the movable end of the mixing motor 205. With such a design, the mixing accommodating portion 203 realizes a shaking action under the drive of the mixing motor 205.

[0092] Furthermore, a first optical coupler 219 is disposed on the mixing seat plate 216 , and an optical coupler baffle 218 matched with the first optical coupler 219 is disposed on the sliding plate 210 for sensing the movement of the sliding plate 20 .

[0093] The mixing accommodation portion 203 is fixedly connected with a mixing optical coupling baffle 202, and the mixing seat plate 216 is provided with a mixing optical coupler 214 matched with the mixing optical coupling baffle 202 through the mixing optical coupling fixing plate 213, for sensing the movement of the mixing accommodation portion 203;

[0094] In this embodiment, a liquid injection mechanism 50 is provided above the mixing mechanism 20 .

[0095] In one embodiment, the magnetic adsorption mechanism 30 is fixed on the mixing mechanism 20; the magnetic adsorption mechanism 30 is used to adsorb the magnetic beads in the reaction cup 70 on the turntable 401 to prevent them from being sucked away by the liquid aspiration mechanism 60. Specifically, Figure 5 As shown, the magnetic adsorption mechanism 30 includes a magnet seat 302, which is annular and detachably connected between the top of the mixing seat plate 216 and the top of the motor fixing seat 217; a magnet 301 is provided on the magnet seat 302, and the magnet 301 is carried on the annular magnet seat 302, and an annular magnet cover plate 303 is connected to the magnet seat 302 to limit and fix the magnet 301. There are multiple magnets 301, and multiple magnets are connected to the annular magnet seat 302 in an annular array.

[0096] In one embodiment, if Figure 6 As shown, the supporting mechanism 40 is connected to the mixing mechanism 20; the supporting mechanism 40 is used to position and support the reaction cup 70, and the supporting mechanism 40 includes a motor seat 406, and a second power unit 408 is connected to the motor seat 406. The second power unit 408 is a motor, which is a prior art and will not be described in detail; the movable end of the second power unit 408 is connected to the second rotating shaft 403. Further, the motor seat 406 is fixedly connected with a second optical coupler 407, and the second rotating shaft 403 is fixedly connected with a second code disk 405 cooperating with the second optical coupler 407, which is used to sense the rotation angle of the second rotating shaft 403; the second rotating shaft 403 is connected with a rotating bearing 404, and the outer ring of the rotating bearing 404 is connected with the annular magnet seat 302 of the mixing mechanism 20, and the motor seat 406 is connected between the mixing seat plate 216 and the motor fixing seat 217; the other end of the second rotating shaft 403 is connected with a turntable 401, and the turntable 401 is provided with a plurality of bearing positions 402 for bearing the reaction cup 70, and as an example, the bearing positions 402 are through holes;

[0097] Specifically, the rotating disk 401 is evenly provided with a plurality of bearing positions 402 along its circumference for bearing the reaction cup 70. The bearing position 402 can be any structure suitable for the shape of the reaction cup. In this embodiment, the bearing position 402 is a through hole. The through holes are evenly arranged along the circumference of the rotating disk 401, and the number is at least 3. In this embodiment, the number of the through holes is 12.

[0098] In one embodiment, the cleaning liquid injection member 507 of the injection mechanism 50 may be a liquid injection needle, a liquid injection tube or other structures capable of injecting cleaning liquid into the reaction cup 70 .

[0099] In this embodiment, the injection mechanism 50 is fixed on the magnetic adsorption mechanism 30; the injection mechanism 50 is used to inject the cleaning liquid into the reaction cup 70. Figure 7 As shown, the injection mechanism 50 includes an injection base 501, and the injection connection base 501 is detachably connected to the magnet base 302 of the magnetic adsorption mechanism 30; the injection connection base 501 is connected to a column 502; the column 502 is connected to an injection base 503, and the injection base 503 is connected to a cleaning liquid injection part 507, and the cleaning liquid injection part 507 is a cleaning liquid injection part injection needle, which is a prior art and will not be described in detail; the cleaning liquid injection part 507 is connected to the magnetic base 302 of the magnetic adsorption mechanism 30; The first injection part positioning seat 504 is connected to the injection base 503, and the first injection part positioning seat 504 is connected to the injection needle pressure plate 505, and the injection needle pressure plate 505 presses and limits the cleaning liquid injection part 507; the first injection part positioning seat 504 is provided with a first through hole for the cooperation of the cleaning liquid injection part 507, which is used for the passing of the cleaning liquid injection part 507; it should be noted that the injection process of the cleaning liquid injection part 507 is a prior art and will not be repeated.

[0100] In one embodiment, the liquid suction component 601 of the magnetic liquid suction mechanism 60 can be a liquid suction needle, a liquid suction tube or other structures that can extract waste liquid from the reaction cup 70 .

[0101] In this embodiment, the liquid suction mechanism 60 is disposed on the mixing mechanism 20; the liquid suction mechanism 60 is used to suck the cleaning liquid in the reaction cup 70, and the liquid suction member 601 moves up and down to suck the liquid; specifically, Figure 8As shown, the magnetic liquid suction mechanism 60 includes a liquid suction rod 602, and the liquid suction rod 602 is connected to the base 215; the liquid suction rod 602 is connected to a screw motor 603, and the screw nut of the screw motor 603 is connected to a screw seat 604; the screw seat 604 is connected to a second longitudinally arranged slide rail 608, and the second slide rail 608 is slidably connected to a second slider 6081, and the second slider 6081 is connected to a slider seat 605; the slider seat 605 is detachably connected to the motor fixing seat 217; the screw seat 604 is connected to a liquid suction optical coupling baffle 606; the slider seat 605 A liquid suction optical coupler 607 is provided on the upper part, which cooperates with the liquid suction optical coupler baffle 606 and is used to sense the action of liquid suction; the upper part of the second slide rail 608 is connected to a liquid suction needle fixing rod 609, and the liquid suction needle fixing rod 609 is connected to a liquid suction needle connecting seat 610, and the liquid suction needle connecting seat 610 is connected to a liquid suction piece 601, and the liquid suction piece 601 is a liquid suction needle, and the liquid suction needle connecting seat 610 is connected to a liquid suction needle pressure block 602, and the liquid suction needle pressure block 602 presses and limits the liquid suction piece 601; the screw motor 603 works, driving the screw seat 604 to rise and fall, and then driving the liquid suction piece 601 to rise and fall relative to the turntable 401. It should be noted that the liquid suction process of the liquid suction piece 601 is a prior art and will not be repeated.

[0102] It also includes a liquid aspiration needle positioning seat 611, which is connected to the liquid injection base 503. The bottom end of the liquid aspiration member 601 moves through the through hole of the liquid aspiration needle positioning seat 611 and cooperates with the corresponding reaction cup 70 to perform liquid aspiration.

[0103] In this embodiment, the multiple magnets 301 on the magnetic adsorption mechanism 30 are used to adsorb the magnetic particle conjugate in the reaction cup 70 to the inner wall of the reaction cup 70 before the liquid is absorbed. One or more magnetic adsorption stations correspond to a cleaning component, and a cleaning liquid injection part 507 of an injection mechanism 50 and a liquid absorption part 601 of a liquid absorption mechanism 60 constitute a cleaning component. In this embodiment, six magnets 301 are evenly distributed circumferentially below the turntable 401, and the six magnets 301 are all arranged on the annular magnet seat 302 between the cleaning liquid injection part 507 and the liquid absorption part 601. When the turntable 401 rotates intermittently, the reaction cup 70 corresponds to the magnet 301 one by one each time the turntable is intermittent. The intermittent time is sufficient to complete the injection of the liquid injection mechanism, the liquid absorption mechanism completes the liquid absorption, the mixing mechanism completes the mixing, and the manipulator completes the grasping and placing of the reaction cup.

[0104] It should be noted here that completing a process from liquid injection to liquid aspiration is called 1-stage cleaning. Generally, magnetic separation cleaning adopts 2-6-stage cleaning. At least 1 group of cleaning components is set, which can be 2 groups, 3 groups, etc. The number of cleaning components in traditional design is equal to the number of cleaning stages.

[0105] In this embodiment, a three-stage cleaning is adopted, and a set of cleaning components is designed to achieve a complete magnetic separation cleaning by cycling three times.

[0106] In one embodiment, if Figure 1-Figure 3 As shown, it also includes a substrate injection station, on which a substrate injection mechanism 80 is provided. The substrate injection mechanism 80 is set at a carrying position of a reaction cup behind the liquid aspiration mechanism, and a mixing component is provided below the substrate injection mechanism 80. In this embodiment, there are two mixing components, one mixing component is used for mixing after the cleaning liquid is injected, and the other mixing component is used for mixing after the substrate is injected; the substrate injection mechanism 80 injects substrate liquid into the reaction cup 70 after cleaning, and shakes it evenly; the substrate liquid is liquid A (the main component of liquid A is luminol) or liquid B (the main component of liquid B is peroxidase) to meet the luminescence detection requirements of different samples.

[0107] like Figure 5 As shown, the substrate injection mechanism 80 includes a liquid A injection needle 506, which is a prior art and will not be described in detail; the liquid A injection needle 506 is connected to the injection base 503 through a second injection part positioning seat 5041, and the liquid A injection needle 506 and the cleaning liquid injection part 507 are arranged at intervals; the second injection part positioning seat 5041 is connected to an injection needle pressure plate 505, and the injection needle pressure plate 505 presses and limits the liquid A injection needle 506; the second injection part positioning seat 5041 is provided with a second through hole for the liquid A injection needle 506 to cooperate with, which is used for the liquid A injection needle 506 to pass through.

[0108] In this embodiment, three cup grabbing stations are provided, and the three cup grabbing stations are located between the liquid injection station and the substrate liquid injection station. When corresponding to three cycles respectively, the robot places a new reaction cup into the magnetic separation cleaning device, among which the third cup grabbing station also serves as the cup discharge station of the magnetic separation cleaning device.

[0109] In some embodiments, the injection station, the pipetting station, the substrate injection station and the cup grabbing station are arranged at intervals along the same circumference of the turntable 401, and the turntable 401 drives the reaction cup 70 to perform circular motion among the injection station, the pipetting station and the substrate injection station.

[0110] Since magnetic separation cleaning is usually multi-stage cleaning, it is a common technical implementation method in the art that the number of cleaning components is equal to the number of cleaning stages. The number of cleaning components in the present invention is less than the number of cleaning stages, which needs to be implemented in conjunction with a preset process and method.

[0111] An immunoassay analyzer according to one embodiment of the present application includes the magnetic separation and cleaning device as described above.

[0112] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0113] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps set forth in these embodiments do not limit the scope of the present application. Meanwhile, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as a part of the specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

[0114] In the description of the present application, it should be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction, and therefore cannot be understood as limiting the scope of protection of the present application; the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0115] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" may include both "above" and "below". The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0116] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of this application.

[0117] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0118] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A magnetic separation cleaning method, characterized in that: It includes the following steps: Transferring reaction cups between the injection station, adsorption station and aspiration station; Injecting a cleaning solution into the reaction cup at the liquid injection station; Adsorbing the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup at the adsorption station, and extracting waste liquid from the reaction cup into which the cleaning liquid has been injected at the liquid aspiration station; The number of liquid injection stations is the same as the number of liquid aspiration stations, and at least one liquid injection station and liquid aspiration station are set; completing one liquid injection, one adsorption, and one liquid aspiration is called one-stage cleaning, and magnetic separation cleaning is 2-6-stage cleaning; The number of injection stations is less than or equal to the number of cleaning stages required to complete a complete magnetic separation cleaning, and the number of cleaning stages required to complete a complete magnetic separation cleaning is at least 2 stages; to complete a complete magnetic separation cleaning, one injection station injects cleaning liquid into at least two reaction cups in succession, and one aspiration station extracts waste liquid into at least two reaction cups in succession.

2. The magnetic separation cleaning method according to claim 1, characterized in that: It also includes a plurality of cup grabbing stations, where the manipulator grabs and places the reaction cup at different cup grabbing stations; the plurality of cup grabbing stations are located between the liquid injection station and the liquid aspiration station.

3. A magnetic separation cleaning device for implementing the magnetic separation cleaning method according to claim 2, characterized in that: It includes: The carrying mechanism comprises a turntable for carrying the reaction cup and being intermittently rotatable, wherein the turntable is evenly provided with a plurality of carrying positions for carrying the reaction cup along its circumference, wherein the carrying positions can take the cup grabbing position as the initial position and go back to the cup grabbing position after at least one stage of cleaning; A liquid injection mechanism, used for injecting cleaning liquid into the reaction cup; A mixing mechanism for shaking the reaction cup; A magnetic adsorption mechanism is used to adsorb the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup; A liquid suction mechanism, used to extract waste liquid from a reaction cup that has been injected with cleaning liquid; The number of the injection mechanisms is equal to the number of the suction mechanisms, and the turntable carries the reaction cup and intermittently rotates between the injection mechanism and the magnetic suction mechanism. When the rotation stops, the magnetic adsorption mechanism adsorbs the magnetic particle conjugate in the reaction cup onto the inner wall of the reaction cup.

4. The magnetic cleaning device according to claim 3, characterized in that: A cleaning liquid injection part of a liquid injection mechanism and a liquid absorbing part of a liquid absorbing mechanism form a cleaning assembly; The number of cleaning components is less than or equal to the number of cleaning stages of magnetic separation cleaning, and the required number of cleaning stages is achieved by carrying the reaction cup through the cleaning liquid injection part and the liquid suction part for cyclic cleaning; The product of the number of gripping cup stations and the number of cleaning components is equal to the number of cleaning stages required to complete a complete magnetic separation cleaning.

5. The magnetic cleaning device according to claim 4, characterized in that: The cleaning method of the magnetic separation cleaning device comprises the following steps: S801, the turntable intermittently rotates to transfer the reaction cup at the first cup grabbing station to the liquid injection station; S802, injecting cleaning solution into the reaction cup at the liquid injection station; S803, the turntable rotates the reaction cup to the adsorption station, adsorbs the magnetic particle binding material suspended in the reaction cup to the inner wall of the supporting reaction cup at the adsorption station, and extracts waste liquid from the reaction cup into which the cleaning liquid has been injected at the liquid aspiration station; S804, the turntable rotates intermittently to transfer the reaction cup to the first cup grabbing station, and the robot puts the reaction cup of the next cycle to the second cup grabbing station; S805, the turntable transfers the first reaction cup to the liquid injection station for second-stage cleaning, and the cycle continues to complete the cleaning stages of magnetic separation cleaning required for the reaction cup.

6. The magnetic cleaning device according to claim 5, characterized in that: The mixing mechanism is arranged above the turntable or the mixing mechanism is arranged below the turntable; The mixing mechanism is used for mixing the reaction cups on the turntable; the mixing mechanism comprises a base, a motor fixing seat is fixedly connected to the base, and a first power unit is connected to the motor fixing seat; the movable end of the first power unit is connected to a first rotating shaft through a coupling to transmit power; the first rotating shaft is rotatably connected to a mixing seat plate arranged on the base, and the first rotating shaft can rotate on the mixing bottom plate; the other end of the first rotating shaft extends to the outside of the mixing bottom plate, and the other end of the first rotating shaft is fixedly connected to an eccentric wheel; There is a distance between the center of the eccentric wheel and the center of the first rotating shaft; a circular slide groove is formed on the eccentric wheel, a cam follower is slidably connected in the circular slide groove, and the cam follower slides in the circular slide groove; a sliding connecting plate is movably connected to the outer end of the cam follower, and the other end of the sliding connecting plate is connected to a sliding plate; The mixing seat plate is fixedly connected to a first slide rail arranged longitudinally, the first slide rail is slidably connected to a second slide block, and the slide plate is fixed to the first slide block; The sliding plate is connected to a mixing assembly, which includes a mixing motor fixing plate connected to the sliding plate, and the mixing motor is connected to the mixing motor fixing plate; the movable end of the mixing motor is connected to a mixing accommodating portion that matches the reaction cup; the center of the mixing accommodating portion does not coincide with the center of the movable end of the mixing motor.

7. The magnetic cleaning device according to claim 6, characterized in that: A liquid injection mechanism is provided above the mixing mechanism; the magnetic adsorption mechanism is fixed on the mixing mechanism; the magnetic adsorption mechanism is used to adsorb the magnetic beads in the reaction cup on the turntable to prevent them from being sucked away by the liquid aspiration mechanism; The magnetic adsorption mechanism includes a magnet seat, which is detachably connected between the top of the mixing seat plate and the top of the motor fixing seat; a magnet is provided on the magnet seat, and the magnet is carried on an annular magnet seat, and an annular magnet cover is connected to the magnet seat to limit and fix the magnet.

8. The magnetic cleaning device according to claim 7, characterized in that: The bearing mechanism is connected to the mixing mechanism; the bearing mechanism is used to position and support the reaction cup, and the bearing mechanism includes a motor seat, a second power unit is connected to the motor seat, and a second rotating shaft is connected to the movable end of the second power unit. A rotating bearing is connected to the second rotating shaft, and the outer ring of the rotating bearing is connected to the annular magnet seat of the mixing mechanism. The motor seat is connected between the mixing seat plate and the motor fixing seat; the other end of the second rotating shaft is connected to a turntable, and the turntable is provided with a plurality of bearing positions for carrying reaction cups; the turntable is evenly provided with a plurality of bearing positions along its circumference for carrying reaction cups.

9. The magnetic cleaning device according to claim 8, characterized in that: The injection mechanism is fixed on the magnetic adsorption mechanism; the injection mechanism is used to inject cleaning liquid into the reaction cup, the injection mechanism includes an injection base, and the injection connecting seat is detachably connected to the magnet seat of the magnetic adsorption mechanism; the injection connecting seat is connected to a column; the column is connected to the injection base, and the injection base is connected to a cleaning liquid injection piece; the cleaning liquid injection piece is connected to the injection base through a first injection piece positioning seat, and the first injection piece positioning seat is connected to an injection needle pressure plate, which presses and limits the cleaning liquid injection piece; the first injection piece positioning seat is provided with a first through hole for the cooperation of the cleaning liquid injection piece, which is used for the passage of the cleaning liquid injection piece.

10. The magnetic cleaning device according to claim 9, characterized in that: The magnetic liquid suction mechanism is arranged on the mixing mechanism; the liquid suction mechanism is used to suck the cleaning liquid in the reaction cup; the magnetic liquid suction mechanism includes a liquid suction vertical rod, and the liquid suction vertical rod is connected to the base; the liquid suction vertical rod is connected to a screw motor, and the screw nut of the screw motor is connected to a screw seat; the screw seat is connected to a longitudinally arranged second slide rail, and a second slider is slidably connected to the second slide rail, and the second slider is connected to a slider seat; the slider seat is detachably connected to the motor fixing seat; the upper part of the second slide rail is connected to a liquid suction needle fixing rod, and the liquid suction needle fixing rod is connected to a liquid suction needle connecting seat, and a liquid suction piece is connected to the liquid suction needle connecting seat, and a liquid suction needle pressure block is connected to the liquid suction needle connecting seat, and the liquid suction needle pressure block presses and limits the liquid suction piece; It also includes a liquid-absorbing needle positioning seat, which is connected to the liquid-injection base. The bottom end of the liquid-absorbing component movably passes through the through hole of the liquid-absorbing needle positioning seat to cooperate with the corresponding reaction cup.

11. The magnetic cleaning device according to claim 10, characterized in that: It also includes a substrate injection station, on which a substrate injection mechanism is provided. The substrate injection mechanism is provided at a bearing position of a reaction cup behind the liquid aspiration mechanism, and a mixing component is provided at the corresponding station. There are two mixing components, one mixing component is used for mixing after the cleaning liquid is injected, and the other mixing component is used for mixing after the substrate is injected; The substrate injection mechanism includes an A-liquid injection needle, which is connected to the injection base through a second injection piece positioning seat, and the A-liquid injection needle and the cleaning liquid injection piece are arranged at an interval; the second injection piece positioning seat is connected to an injection needle pressure plate, and the injection needle pressure plate presses and limits the A-liquid injection needle; the second injection piece positioning seat is provided with a second through hole for the A-liquid injection needle to cooperate with, which is used for the A-liquid injection needle to pass through.

12. The magnetic cleaning device according to claim 11, characterized in that: The liquid injection station, the liquid aspiration station, the substrate liquid injection station and the cup grabbing station are arranged at intervals along the same circumference of the turntable, and the turntable drives the reaction cup to perform circular motion among the cup grabbing station, the liquid injection station, the liquid aspiration station and the substrate liquid injection station.

13. An immunoassay analyzer, characterized in that: It comprises a magnetic cleaning device as described in any one of claims 3-12.

Images