Combined fillers for biological deodorization and method for their preparation

Abstract

The application provides a combined filler for biological deodorization and a preparation method thereof, and relates to the technical field of water treatment. The preparation method of the combined filler for biological deodorization has the characteristics of simple operation and stable operation, and the prepared combined filler has high strength, long service life, easy penetration of nutrients and easy proliferation of microorganisms. Through the cooperation between the fillers, the main foul-smelling gases such as VOCs, H2S and NH3 in sewage can be quickly and effectively removed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of water treatment, and particularly relates to a combined filler for biological deodorization and a preparation method thereof. BACKGROUND

[0002] With the progress of society and the improvement of living standards, the public demand for a good ecological environment is also increasingly high, and the influence of odor gas generated in the sewage treatment process on atmospheric pollution and the surrounding environment is increasingly concerned. At present, the odor pollution control technology of sewage treatment plants mainly includes "washing absorption method, biological method, adsorption method, advanced oxidation method, masking method" and the like. Among them, the biological deodorization technology has the advantages of low treatment cost, good effect and no secondary pollution, and is the most widely used deodorization technology in sewage treatment plants. However, it is easily affected by changes in pollution load and components, non-steady state conditions and external environmental temperature, and has problems such as unstable treatment effect and low removal rate of pollutants (especially organic pollutants).

[0003] The core of the biological deodorization technology is the biological filler and the microbial community. Therefore, improving the biological filler and increasing the abundance and activity of high-efficiency deodorization microorganisms are the key to the depth and stability of odor gas removal. The existing deodorization biological fillers can be divided into three categories: inorganic fillers, organic fillers and composite fillers. Among them, the inorganic fillers commonly used are bamboo charcoal, ceramic balls and volcanic rocks; the organic fillers commonly used are artificial synthetic organic materials such as polyolefin and polyurethane, and natural organic materials such as tree bark, coconut shell and straw; the composite fillers are simple mixtures or compression molding of inorganic fillers and organic fillers. The inorganic fillers and artificial synthetic organic materials have the advantages of high strength and long service life, but also have the disadvantages of lack of nutrients required for microbial growth, slow biofilm formation and unstable treatment effect. The natural organic fillers and composite fillers are easy for microbial proliferation, but have the problems of low strength, short service life and secondary pollution caused by organic matter corruption.

[0004] At present, CN113305731A and CN102910727B provide a method for preparing deodorizing biological filler by adding nutrient agents and slow-release agents in the process of artificial sintering ceramsite polishing, which proposes that the deodorizing biological filler uses high-strength and long-life ceramsite material as a substrate, and adds nutrient substances and slow-release agents to improve the utilization efficiency and period of nutrient substances, which is beneficial to improve the nutrient substances required by microorganisms and thus beneficial to the proliferation of microorganisms. However, it is found through multiple field practices and theoretical proofs that the above-mentioned technology has the problem that the pore structure of the ceramsite material is not conducive to the penetration of nutrient substances, resulting in a low load of nutrient substances. In addition, the ceramsite after high-temperature sintering is mostly inert oxide, which is not easy to be utilized by microorganisms. In addition, the above-mentioned technology also has the defects of incomplete addition of nutrient elements and non-addition of special microorganisms. CN114177767 A provides a method for preparing a biological filler embedded with bacillus by a natural porous ore. However, bacillus does not have a specific effect on hydrogen sulfide, ammonia and other main malodorous gases produced by sewage plants.

[0005] Therefore, there is an urgent need to provide a new biological deodorizing filler.

[0006] In view of this, the present application is proposed. SUMMARY

[0007] The first object of the present application is to provide a preparation method of a combined filler for biological deodorization to solve the above-mentioned technical problems.

[0008] The second object of the present application is to provide a combined filler for biological deodorization.

[0009] In order to achieve the above-mentioned objects, the following technical solutions are adopted:

[0010] In a first aspect, the present application provides a preparation method of a combined filler for biological deodorization, wherein the combined filler comprises V filler, H filler, N filler and Q filler;

[0011] The bacterial flora loaded on the V filler comprises at least one of Pseudomonas phenylpyruvica, Pseudomonas stutzeri, yeast, Desulfomonas phenolica or Alcaligenes;

[0012] The bacterial flora loaded on the H filler comprises at least one of Rhodopseudomonas palustris, Geobacter, Chromatium or Sulfolobus acidophilus;

[0013] The bacterial flora loaded on the N filler comprises nitrifying bacteria;

[0014] The bacterial flora loaded on the Q filler comprises at least one of Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia;

[0015] The preparation method of the V filler, H filler, N filler or Q filler comprises the following steps:

[0016] a. embedding the bacterial suspension with polyvinyl alcohol as the embedding agent to obtain a polyvinyl alcohol-embedded bacterial suspension;

[0017] b. mixing polypropylene, activated carbon and sodium alginate, then adding a polypropylene foaming agent for foaming treatment to obtain a foaming product;

[0018] c. mixing the foaming product obtained in step b and the polyvinyl alcohol-embedded bacterial suspension prepared in step a, then performing compression molding and crosslinking in a mold to obtain a V filler, an H filler, an N filler or a Q filler;

[0019] The V filler, the H filler, the N filler and the Q filler each independently comprise, in terms of mass fraction, 50-70 parts of polypropylene, 5-10 parts of activated carbon, 5-10 parts of sodium alginate and 10-20 parts of the polyvinyl alcohol-embedded bacterial suspension.

[0020] As a further technical solution, in step a, the step of embedding the bacterial suspension with polyvinyl alcohol as the embedding agent is as follows:

[0021] After the bacteria are inoculated in a culture solution and cultured to the late logarithmic phase, the bacteria are mixed with polyvinyl alcohol to obtain a polyvinyl alcohol-embedded bacterial suspension.

[0022] As a further technical solution, the culture solution of the bacterial suspension of the V filler comprises peptone, beef extract, sodium chloride and water;

[0023] In terms of mass fraction, the raw materials of the polyvinyl alcohol-embedded bacterial suspension in the V filler comprise, in terms of mass fraction, 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0024] As a further technical solution, the culture solution of the bacterial suspension of the H filler comprises yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate and water;

[0025] In terms of mass fraction, the raw materials of the polyvinyl alcohol-embedded bacterial suspension in the H filler comprise, in terms of mass fraction, 20-30 parts of polyvinyl alcohol, 5-10 parts of yeast extract, 0.5-2 parts of ammonium chloride, 0.5-2 parts of dipotassium hydrogen phosphate and 36-64 parts of water.

[0026] As a further technical solution, the culture solution of the bacterial suspension of the N filler comprises peptone, beef extract, sodium chloride and water;

[0027] In terms of mass fraction, the raw materials of the polyvinyl alcohol-embedded bacterial suspension in the N filler comprise, in terms of mass fraction, 15-25 parts of polyvinyl alcohol, 10-20 parts of peptone, 2-7 parts of beef extract, 3-5 parts of sodium chloride and 50-70 parts of water.

[0028] As a further technical solution, the culture solution of the bacterial suspension of the Q filler comprises peptone, beef extract, sodium chloride and water.

[0029] In the Q filler, the raw materials of the bacterial suspension embedded by polyvinyl alcohol comprise, by mass fraction, polyvinyl alcohol 20-30 parts, peptone 5-15 parts, beef extract 10-30 parts, sodium chloride 5-10 parts and water 15-60 parts.

[0030] As a further technical solution, the combined filler comprises, by mass fraction, V filler 20-40 parts, H filler 15-35 parts, N filler 10-30 parts and Q filler 5-25 parts.

[0031] As a further technical solution, the bacterial population loaded by the V filler is Pseudomonas phenylpyruvica, Pseudomonas stutzeri, yeast, Desulfobacterium phenolicum and Alcaligenes.

[0032] The bacterial population loaded by the H filler is Rhodopseudomonas palustris, Thiocapsa, Chromatium and Sulfolobus acidocaldarius.

[0033] The bacterial population loaded by the Q filler is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

[0034] In a second aspect, the application provides a combined filler for biological deodorization, which is prepared by the above preparation method.

[0035] In a third aspect, the application provides application of the above combined filler in sewage treatment.

[0036] Compared with the prior art, the application has the following beneficial effects:

[0037] The preparation method of the combined filler for biological deodorization provided by the application has the characteristics of simple operation and smooth operation. The combined filler prepared by the method has high strength and long service life, is easy to penetrate nutrients and proliferate microorganisms, and can quickly and effectively remove main malodorous gases such as VOCs, H2S and NH3 in sewage through the cooperation of the fillers. DETAILED DESCRIPTION

[0038] The embodiments of the application will be described in detail below with reference to the embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only used to illustrate the application and should not be regarded as limiting the scope of the application. Based on the examples in the application, all other examples obtained by those skilled in the art without creative labor are within the scope of protection of the application. If the specific conditions are not specified, the conventional conditions or the conditions recommended by the manufacturer are used. If the reagents or instruments used are not specified by the manufacturer, they are all conventional products that can be purchased on the market.

[0039] In a first aspect, the present application provides a preparation method of a combined filler for biological deodorization, wherein the combined filler comprises a V filler, an H filler, an N filler and a Q filler.

[0040] The V filler loaded bacterial flora comprises at least one of Pseudomonas phenazinium, Pseudomonas stutzeri, Saccharomycetes, Thioalkalivibrio phenodioxidans or Alcaligenes, and is mainly used for absorbing and removing VOCs.

[0041] The H filler loaded bacterial flora comprises at least one of Rhodopseudomonas palustris, Chloronema, Chromatium or Sulfolobus acidocaldarius, and is mainly used for absorbing and removing H2S.

[0042] The N filler loaded bacterial flora comprises nitrifying bacteria, and is mainly used for absorbing and removing NH3.

[0043] The Q filler loaded bacterial flora comprises at least one of Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia, and is used for absorbing and removing malodorous gases such as methyl mercaptan, dimethyl disulfide and trimethylamine.

[0044] The preparation method of the V filler, the H filler, the N filler or the Q filler comprises the following steps:

[0045] a. embedding a bacterial suspension with polyvinyl alcohol as an embedding agent to obtain a polyvinyl alcohol embedded bacterial suspension;

[0046] b. mixing polypropylene, activated carbon and sodium alginate, then adding a polypropylene foaming agent for foaming treatment to obtain a foaming product;

[0047] c. mixing the foaming product obtained in step b and the polyvinyl alcohol embedded bacterial suspension prepared in step a, then performing compression molding and crosslinking in a mold to obtain the V filler, the H filler, the N filler or the Q filler;

[0048] In the V filler, the H filler, the N filler and the Q filler, the mass fraction of polypropylene may be, but is not limited to, 50 parts, 60 parts or 70 parts; the mass fraction of activated carbon may be, but is not limited to, 5 parts, 8 parts or 10 parts; the mass fraction of sodium alginate may be, but is not limited to, 5 parts, 8 parts or 10 parts; and the mass fraction of the polyvinyl alcohol embedded bacterial suspension may be, but is not limited to, 10 parts, 15 parts or 20 parts.

[0049] The preparation method of the combined filler for biological deodorization has the characteristics of simple operation and stable operation.

[0050] In some optional embodiments, in step a, the step of embedding the bacterial suspension with polyvinyl alcohol as an embedding agent is as follows:

[0051] The bacteria are inoculated into a culture solution and cultured to the late logarithmic phase, and then mixed with polyvinyl alcohol to obtain a polyvinyl alcohol-embedded bacterial suspension.

[0052] It should be noted that the required inoculation is selected according to the type of bacterial population loaded in the filler.

[0053] In some alternative embodiments, if the filler includes multiple bacteria, preferably each bacterium is separately cultured to the late logarithmic phase, and then mixed in equal volumes, and then polyvinyl alcohol is added for embedding.

[0054] In some alternative embodiments, the culture solution of the bacterial suspension of the V filler includes peptone, beef extract, sodium chloride, and water.

[0055] In the V filler, the raw materials of the polyvinyl alcohol-embedded bacterial suspension include, by mass fraction: polyvinyl alcohol 15-25 parts, peptone 10-20 parts, beef extract 2-7 parts, sodium chloride 3-5 parts, and water 50-70 parts.

[0056] In some alternative embodiments, the culture solution of the bacterial suspension of the H filler includes yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate, and water.

[0057] In the H filler, the raw materials of the polyvinyl alcohol-embedded bacterial suspension include, by mass fraction: polyvinyl alcohol 20-30 parts, yeast extract 5-10 parts, ammonium chloride 0.5-2 parts, dipotassium hydrogen phosphate 0.5-2 parts, sodium bicarbonate 10-20 parts, and water 36-64 parts.

[0058] In some alternative embodiments, in the H filler, the raw materials of the polyvinyl alcohol-embedded bacterial suspension further include sodium bicarbonate 10-20 parts; the sodium bicarbonate is used as a protective agent and is added to the bacterial suspension at the same time as the polyvinyl alcohol in step a.

[0059] In some alternative embodiments, the culture solution of the bacterial suspension of the N filler includes peptone, beef extract, sodium chloride, and water.

[0060] In the N filler, the raw materials of the polyvinyl alcohol-embedded bacterial suspension include, by mass fraction: polyvinyl alcohol 15-25 parts, peptone 10-20 parts, beef extract 2-7 parts, sodium chloride 3-5 parts, and water 50-70 parts.

[0061] In some alternative embodiments, the culture solution of the bacterial suspension of the Q filler includes peptone, beef extract, sodium chloride, and water.

[0062] In the Q filler, the raw materials of the polyvinyl alcohol-embedded bacterial suspension include, by mass fraction: polyvinyl alcohol 20-30 parts, peptone 5-15 parts, beef extract 10-30 parts, sodium chloride 5-10 parts, and water 15-60 parts.

[0063] The present application designs culture media suitable for the cultivation of different bacterial species to promote the proliferation of the bacterial species.

[0064] In some alternative embodiments, the combined filler includes V filler 20-40 parts, H filler 15-35 parts, N filler 10-30 parts and Q filler 5-25 parts by mass fraction.

[0065] By the cooperation of various fillers, the main malodorous gases such as VOCs, H2S and NH3 in sewage can be better removed.

[0066] In some alternative embodiments, the V filler loaded bacterial flora is Pseudomonas mesophila, Pseudomonas stutzeri, yeast, Desulfobacterium phenolicum and Alcaligenes;

[0067] The H filler loaded bacterial flora is Rhodopseudomonas palustris, Geobacter, Chromatium and Thiobacterium thiooxidans;

[0068] The Q filler loaded bacterial flora is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

[0069] The inventor found that the above-mentioned combination of multiple bacterial flora has better deodorizing effect than single bacterial flora.

[0070] In a second aspect, the present application provides a combined filler for biological deodorization, which is prepared by the above preparation method.

[0071] The combined filler prepared by the present application has high strength, long service life, easy penetration of nutrients and microbial proliferation, and can quickly and effectively remove the main malodorous gases such as VOCs, H2S and NH3 in sewage through the cooperation of various fillers.

[0072] In a third aspect, the present application provides the application of the above-mentioned combined filler in sewage treatment.

[0073] The present application will be further illustrated by specific examples and comparative examples, but it should be understood that these examples are only for more detailed illustration and should not be understood as limiting the present application in any form.

[0074] Comparative Example 1

[0075] A preparation method of a combined filler for biological deodorization (without adding activated carbon):

[0076] Preparation of V filler:

[0077] Pseudomonas phenazinium, Pseudomonas stutzeri, Desulfobulbus phenoxidiants were respectively cultured in the formula of peptone 10 parts, beef extract 5 parts, sodium chloride 5 parts, water 60 parts to the late logarithmic phase, then the three kinds of bacterial suspension were mixed in equal amounts, and 60 parts of polyvinyl alcohol was mixed in, and then it was used after mixing evenly;

[0078] 70 parts of polypropylene (PP), 10 parts of sodium alginate were mixed evenly, and a commercially available PP foaming agent was added, and then it was heated to 220±10℃ in a foaming machine to make the PP mixture expand and form a porous structure. The preliminarily foamed PP mixture was put into 20 parts of V filler bacterial population which was immobilized, and then it was soaked and mixed evenly. After that, it was put into a mold and pressed to form a shape. During the pressing process, the V filler bacterial population embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and combined firmly. After the pressing was completed, the V filler was obtained, which was cut into 2×2 mm blocks and used.

[0079] H filler preparation:

[0080] Rhodopseudomonas palustris and Thiobacterium thiooxidans were respectively cultured in the formula of yeast extract 10 parts, ammonium chloride 1 part, dipotassium hydrogen phosphate 1 part, water 53 parts to the late logarithmic phase, and then the two kinds of bacterial suspensions were mixed in equal amounts, and 40 parts of polyvinyl alcohol and 30 parts of sodium bicarbonate were mixed in, and then it was used after mixing evenly;

[0081] 70 parts of polypropylene (PP), 10 parts of sodium alginate were mixed evenly, and a commercially available PP foaming agent was added, and then it was heated to 220±10℃ in a foaming machine to make the PP mixture expand and form a porous structure. The preliminarily foamed PP mixture was put into 20 parts of H filler bacterial population which was immobilized, and then it was soaked and mixed evenly. After that, it was put into a mold and pressed to form a shape. During the pressing process, the H filler bacterial population embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and combined firmly. After the pressing was completed, the H filler was obtained, which was cut into 3×3 mm blocks and used.

[0082] N filler preparation:

[0083] Nitrifying bacteria were cultured in the medium of peptone 10 parts, beef extract 5 parts, sodium chloride 5 parts, water 60 parts to the late logarithmic phase, and then 20 parts of polyvinyl alcohol was mixed in, and the cultured bacterial suspension was embedded and protected for use;

[0084] 70 parts of polypropylene (PP), 10 parts of sodium alginate, mixed evenly, add commercially available PP foaming agent, put into the foaming machine for heating treatment to 220±10℃, the PP mixture expands, forming a porous structure, the preliminary foaming of PP mixture into the fixed 20 parts of Q filler microbial population, soaking mixed evenly, put into the mold after compression molding, the compression process of polyvinyl alcohol embedded Q filler microbial population and PP mixture crosslinking fully, combined firmly, after the completion of the Q filler, cut into 2x2mm block size for use.

[0085] Q filler preparation:

[0086] Bacillus subtilis, Lactobacillus plantarum, Nocardia were respectively cultured in the formula of 10 parts of proteose peptone, 15 parts of beef extract, 7 parts of sodium chloride, 43 parts of water to the late logarithmic phase, the three kinds of bacterial suspension were mixed in equal amounts, and 75 parts of polyvinyl alcohol was mixed at the same time, and then used;

[0087] 70 parts of polypropylene (PP), 10 parts of sodium alginate, mixed evenly, add commercially available PP foaming agent, put into the foaming machine for heating treatment to 220±10℃, the PP mixture expands, forming a porous structure, the preliminary foaming of PP mixture into the fixed 20 parts of Q filler microbial population, soaking mixed evenly, put into the mold after compression molding, the compression process of polyvinyl alcohol embedded Q filler microbial population and PP mixture crosslinking fully, combined firmly, after the completion of the Q filler, cut into 2x2mm block size for use.

[0088] Combined use:

[0089] The original deodorization equipment of a sewage treatment plant used ceramic filter as filler. After the original system had been running for 2 years, the H2S was 0.56-0.68 mg / m 3 , NH3 was 3.5-6.7 mg / m 3 , and odor concentration was 60-72 at the monitoring point of the sewage plant, which could not stably reach the third level of the factory boundary in the "Odor Pollutant Emission Standard" (GB14554-93). Therefore, the original deodorization system ceramic filter was replaced by the combination of 30 parts of V filler without activated carbon, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler. After running for 10 days, the odor at the monitoring point was significantly reduced. After 10 days of running, the odor was significantly reduced, and the odor detection was carried out at the monitoring point for 20 days. The detection showed that the H2S was 0.03-0.10 mg / m 3 , NH3 was 0.9-1.7 mg / m 3, the odor concentration is 6-15, which can reach the factory boundary secondary standard in the "Odor Pollutant Discharge Standard" (GB14554-93), indicating that the prepared combined functional biological filler without activated carbon has excellent performance and can meet the deodorization function.

[0090] Comparative Example 2

[0091] A preparation method of a combined filler for biological deodorization (single strain filler):

[0092] Preparation of V filler:

[0093] The Pseudomonas putida is cultured in a formula of 10 parts of proteose peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water to the late logarithmic phase, then 20 parts of polyvinyl alcohol are mixed, the polyvinyl alcohol is used as an embedding agent of the V filler bacterial population, and after mixing, the prepared bacterial suspension is embedded and protected for use;

[0094] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate are uniformly mixed, a commercially available PP foaming agent is added, and heating treatment is performed in a foaming machine to 220±10 DEG C, so that the PP mixture is expanded to form a porous structure, the preliminarily foamed PP mixture is placed in the 20 parts of the immobilized V filler bacterial population, and after uniform soaking and mixing, it is placed in a mold and pressed to form a shape, in the pressing process, the V filler bacterial population embedded by the polyvinyl alcohol and the PP mixture are fully crosslinked and firmly combined, and after the pressing is completed, the V filler is prepared, which is cut into a block with a size of 2*2 mm for use.

[0095] Preparation of H filler:

[0096] The Rhodopseudomonas palustris is cultured in a formula of 10 parts of yeast extract, 1 part of ammonium chloride, 1 part of dipotassium hydrogen phosphate, and 53 parts of water to the late logarithmic phase, then 20 parts of polyvinyl alcohol and 15 parts of sodium bicarbonate are mixed, the polyvinyl alcohol is used as an embedding agent of the V filler bacterial population, and the sodium bicarbonate is used as a H filler protective agent, and after mixing, the prepared bacterial suspension is embedded and protected for use;

[0097] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate are uniformly mixed, a commercially available PP foaming agent is added, and heating treatment is performed in a foaming machine to 220±10 DEG C, so that the PP mixture is expanded to form a porous structure, the preliminarily foamed PP mixture is placed in the 20 parts of the immobilized V filler bacterial population, and after uniform soaking and mixing, it is placed in a mold and pressed to form a shape, in the pressing process, the V filler bacterial population embedded by the polyvinyl alcohol and the PP mixture are fully crosslinked and firmly combined, and after the pressing is completed, the V filler is prepared, which is cut into a block with a size of 2*2 mm for use.

[0098] Preparation of N filler:

[0099] The nitrifying bacteria are cultured in a medium containing 10 parts of proteose peptone, 5 parts of beef extract, 5 parts of sodium chloride, and 60 parts of water to the late logarithmic phase, and 20 parts of polyvinyl alcohol are mixed in as the embedding agent for the V filler bacterial population. After mixing, the bacterial suspension is embedded and protected for later use.

[0100] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate are mixed uniformly, a commercially available PP foaming agent is added, and heating treatment is performed in a foaming machine to 220±10℃, so that the PP mixture is expanded to form a porous structure. The preliminarily foamed PP mixture is placed in the 20 parts of N filler bacterial population that has been immobilized, and after uniform mixing by soaking, it is placed in a mold and compression molded. During the compression process, the N filler bacterial population embedded by polyvinyl alcohol and the PP mixture are fully crosslinked and firmly combined. After the compression is completed, the N filler is obtained, which is cut into 3×3 mm blocks for later use.

[0101] Q filler preparation:

[0102] The Bacillus subtilis is cultured in a formula containing 10 parts of proteose peptone, 15 parts of beef extract, 7 parts of sodium chloride, and 43 parts of water to the late logarithmic phase, and then 25 parts of polyvinyl alcohol are mixed in as the embedding agent for the V filler bacterial population. After mixing, the bacterial suspension is embedded and protected for later use.

[0103] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate are mixed uniformly, a commercially available PP foaming agent is added, and heating treatment is performed in a foaming machine to 220±10℃, so that the PP mixture is expanded to form a porous structure. The preliminarily foamed PP mixture is placed in the 20 parts of N filler bacterial population that has been immobilized, and after uniform mixing by soaking, it is placed in a mold and compression molded. During the compression process, the N filler bacterial population embedded by polyvinyl alcohol and the PP mixture are fully crosslinked and firmly combined. After the compression is completed, the N filler is obtained, which is cut into 3×3 mm blocks for later use.

[0104] Combined use:

[0105] The original deodorization equipment of a sewage treatment plant used ceramic filter as the filler. After the original system had been operated for 2 years, continuous detection was performed at the monitoring point of the sewage plant interface twice a day for 1 week, and the H2S was 0.56-0.68 mg / m 3 , and the NH3 was 3.5-6.7 mg / m 3, the odor concentration is 60-72, and the third level standard of the factory boundary in the "Odorous Pollutant Discharge Standard" (GB14554-93) cannot be stably reached. Therefore, the second time, 30 parts of single-strain V filler, 30 parts of H filler, 20 parts of N filler and 20 parts of Q filler are combined in turn to replace the first time of the combination filler without activated carbon. It is found that the performance of the combined filler is relatively rapid, and the odor at the monitoring point of the factory boundary is obviously reduced after 7 days of operation. After 7 days of operation, the odor is obviously reduced, and the odor detection is carried out at the specified monitoring point every day for 23 days. After detection, H2S is 0.10-0.20 mg / m 3 , NH3 is 1.3-2.6 mg / m 3 , the odor concentration is 15-36, and the third level standard of the factory boundary in the "Odorous Pollutant Discharge Standard" (GB14554-93) can be stably reached. It is indicated that the starting speed of the single-strain combined functional biological filler prepared by the application is slightly faster than that of the activated carbon combined filler, but the deodorization effect is slightly lower than that of the multi-strain combined filler without the cooperation of various deodorization functional bacteria.

[0106] Example 1

[0107] A combined filler preparation method for biological deodorization (containing activated carbon multi-strain filler):

[0108] V filler preparation:

[0109] Pseudomonas phenylpyruvica, Pseudomonas stutzeri and Thiobacillus phenolicus are respectively cultured in a formula of 10 parts of proteose peptone, 5 parts of beef extract, 5 parts of sodium chloride and 60 parts of water to the late logarithmic phase of the bacterial strain, and the three bacterial suspensions are mixed in equal amounts. 60 parts of polyvinyl alcohol are mixed, and the cultured bacterial suspension is embedded and protected for use;

[0110] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon and 10 parts of sodium alginate are uniformly mixed, a commercially available PP foaming agent is added, and heating treatment is carried out in a foaming machine to 220±10℃, so that the PP mixture expands to form a porous structure. The preliminarily foamed PP mixture is placed in 20 parts of V filler bacteria group for immersion and mixing, and then placed in a mold for compression molding. The V filler bacteria group embedded by polyvinyl alcohol and the PP mixture are fully crosslinked and combined firmly during the compression process. After compression molding, V filler is prepared, which is cut into 2×2mm blocks for use.

[0111] H filler preparation:

[0112] Respectively, the moor red false single coccus, sulfur bacteria in the yeast paste 10 parts, ammonium chloride 1 part, potassium phosphate dibasic 1 part, water 53 parts of the formula to the late logarithmic phase of the strain, two kinds of bacteria suspension mixed with equal amount, mixed into polyvinyl alcohol 40 parts, sodium bicarbonate 30 parts, sodium bicarbonate as H filler protectant, mixed evenly after embedding the culture of bacteria suspension protection for later use;

[0113] 60 parts of polypropylene (PP), 10 parts of powder activated carbon, sodium alginate 10 parts, mixed evenly, add commercially available PP foaming agent, put into the foaming machine for heating treatment to 220±10℃, make the PP mixture expand, form a porous structure, put the preliminary foaming PP mixture into the 20 parts of H filler bacteria group which has been immobilized, soak and mix evenly, then put into the mold and press into shape, the H filler bacteria group and PP mixture embedded by polyvinyl alcohol are fully crosslinked and combined firmly during the pressing process, after pressing, H filler is obtained, which is cut into 3×3mm block size for later use.

[0114] N filler preparation:

[0115] The nitrifying bacteria were cultured in a medium containing 10 parts of proteose peptone, 5 parts of beef extract, 5 parts of sodium chloride and 60 parts of water to the late logarithmic phase of the strain, and then 20 parts of polyvinyl alcohol was mixed, and the culture of bacteria suspension was embedded and protected for later use;

[0116] 60 parts of polypropylene (PP), 10 parts of powder activated carbon, sodium alginate 10 parts, mixed evenly, add commercially available PP foaming agent, put into the foaming machine for heating treatment to 220±10℃, make the PP mixture expand, form a porous structure, put the preliminary foaming PP mixture into the 20 parts of H filler bacteria group which has been immobilized, soak and mix evenly, then put into the mold and press into shape, the H filler bacteria group and PP mixture embedded by polyvinyl alcohol are fully crosslinked and combined firmly during the pressing process, after pressing, H filler is obtained, which is cut into 3×3mm block size for later use.

[0117] Q filler preparation:

[0118] Respectively, the moor red false single coccus, sulfur bacteria in the yeast paste 10 parts, ammonium chloride 1 part, potassium phosphate dibasic 1 part, water 53 parts of the formula to the late logarithmic phase of the strain, two kinds of bacteria suspension mixed with equal amount, mixed into polyvinyl alcohol 40 parts, sodium bicarbonate 30 parts, sodium bicarbonate as H filler protectant, mixed evenly after embedding the culture of bacteria suspension protection for later use;

[0119] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, 10 parts of sodium alginate are mixed uniformly, a commercially available PP foaming agent is added, and the mixture is put into a foaming machine for heating treatment to 220±10℃, so that the PP mixture is expanded to form a porous structure. The preliminarily foamed PP mixture is put into 20 parts of immobilized Q filler bacterial population, and after being soaked and mixed uniformly, it is put into a mold for compression molding. During the compression process, the Q filler bacterial population embedded by polyvinyl alcohol and the PP mixture are fully crosslinked, and are combined firmly. After the compression is completed, the Q filler is obtained, which is cut into 2*2mm blocks for use.

[0120] Combination:

[0121] The original deodorization equipment of a sewage treatment plant uses ceramic filter as the filler. After the original system has been operated for 2 years, the H2S is 0.56-0.68mg / m 3 , the NH3 is 3.5-6.7mg / m 3 , and the odor concentration is 60-72 at the monitoring point of the sewage plant, which cannot stably reach the third level standard of the plant boundary in the “Standard for Odor Pollutant Discharge” (GB14554-93). Therefore, the third time, the active carbon and multi-bacterial population are combined with V filler 30 parts, H filler 30 parts, N filler 20 parts and Q filler 20 parts in turn to replace the second single-strain combined filler. It is found that the performance of the combined filler starts quickly, and the odor at the monitoring point is obviously reduced after 4 days of operation. After the odor is obviously reduced after 4 days of operation, the odor detection is carried out at the monitoring point for 26 days of continuous detection twice a day. After detection, the H2S is 0.03-0.08mg / m 3 , the NH3 is 0.8-1.4mg / m 3 , and the odor concentration is 6-13, which can stably reach the second level standard of the plant boundary in the “Standard for Odor Pollutant Discharge” (GB14554-93), indicating that the active carbon and multi-bacterial population combined functional biological filler prepared by the present application has excellent performance, fast starting speed and good deodorization effect.

[0122] Example 2

[0123] A combined filler preparation method for biological deodorization (containing active carbon and multi-strain filler):

[0124] Preparation of V filler:

[0125] Pseudomonas putida, Pseudomonas stutzeri and yeast are respectively cultured in a formula of 15 parts of proteose peptone, 2 parts of beef extract, 3 parts of sodium chloride and 50 parts of water to the late logarithmic phase of the bacterial strains, and then the three bacterial suspensions are mixed in equal amounts, 45 parts of polyvinyl alcohol is mixed, and the cultured bacterial suspension is embedded and protected for use;

[0126] 50 parts of polypropylene (PP), 5 parts of powdered activated carbon, 5 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and the mixture was put into a foaming machine for heating treatment to 220±10°C, so that the PP mixture was expanded to form a porous structure. The preliminarily foamed PP mixture was put into 10 parts of V filler bacteria group for which immobilization was completed, and then soaked and mixed uniformly. After that, the mixture was put into a mold for compression molding. During the compression process, the V filler bacteria group embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and combined firmly. After the compression was completed, the V filler was prepared, which was cut into 2×2 mm blocks for use.

[0127] H filler preparation:

[0128] The mud green bacteria and the coloring bacteria were respectively cultured in a formula of 5 parts of yeast extract, 0.5 parts of ammonium chloride, 0.5 parts of dipotassium hydrogen phosphate, and 36 parts of water to the late logarithmic phase of the bacterial strains. The suspensions of the two kinds of bacteria were mixed in equal amounts, and 50 parts of polyvinyl alcohol and 20 parts of sodium bicarbonate were simultaneously mixed in. The sodium bicarbonate was used as a H filler protective agent. After the mixture was uniformly mixed, the cultured bacterial suspension was embedded and protected for use.

[0129] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and the mixture was put into a foaming machine for heating treatment to 220±10°C, so that the PP mixture was expanded to form a porous structure. The preliminarily foamed PP mixture was put into 20 parts of H filler bacteria group for which immobilization was completed, and then soaked and mixed uniformly. After that, the mixture was put into a mold for compression molding. During the compression process, the H filler bacteria group embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and combined firmly. After the compression was completed, the H filler was prepared, which was cut into 3×3 mm blocks for use.

[0130] N filler preparation:

[0131] The nitrifying bacteria were cultured in a culture medium of 15 parts of proteose peptone, 2 parts of beef extract, 3 parts of sodium chloride, and 50 parts of water to the late logarithmic phase of the bacterial strains. Then, 15 parts of polyvinyl alcohol was mixed in. After the mixture was uniformly mixed, the cultured bacterial suspension was embedded and protected for use.

[0132] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and the mixture was put into a foaming machine for heating treatment to 220±10°C, so that the PP mixture was expanded to form a porous structure. The preliminarily foamed PP mixture was put into 20 parts of H filler bacteria group for which immobilization was completed, and then soaked and mixed uniformly. After that, the mixture was put into a mold for compression molding. During the compression process, the H filler bacteria group embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and combined firmly. After the compression was completed, the H filler was prepared, which was cut into 3×3 mm blocks for use.

[0133] Q filler preparation:

[0134] Bacillus, Lactobacillus plantarum, and beer yeast are respectively cultured in a formula of 5 parts of proteose peptone, 10 parts of beef extract, 5 parts of sodium chloride, and 15 parts of water to the late logarithmic phase, and the three bacterial suspensions are mixed in equal amounts, and polyvinyl alcohol 60 parts is mixed in, and the mixed bacterial suspension is embedded and protected for use;

[0135] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate are uniformly mixed, a commercially available PP foaming agent is added, and the mixture is heated to 220±10℃ in a foaming machine to make the PP mixture expand and form a porous structure. The preliminarily foamed PP mixture is placed in the 20 parts of Q filler bacterial population for immobilization to soak and mix uniformly, and then is placed in a mold for compression molding. During the compression molding process, the Q filler bacterial population embedded by polyvinyl alcohol and the PP mixture are fully crosslinked and firmly combined. After the compression molding is completed, the Q filler is obtained, which is cut into 2×2 mm blocks for use.

[0136] Combination use:

[0137] The original deodorization equipment of a sewage treatment plant used ceramic filter as the filler. After the original system was operated for 2 years, the H2S was 0.56-0.68 mg / m 3 , the NH3 was 3.5-6.7 mg / m 3 , and the odor concentration was 60-72 at the monitoring point of the sewage plant, which could not stably reach the third level of the factory boundary in the “Odor Pollutant Emission Standard” (GB14554-93). The V filler 20 parts, the H filler 30 parts, the N filler 30 parts, and the Q filler 20 parts in Example 2 were combined in sequence to replace the combined filler in Example 1. It was found that the performance of the combined filler started quickly, and the odor at the monitoring point was significantly reduced after 6 days of operation. After the odor was significantly reduced after 6 days of operation, the odor detection was performed at the monitoring point for 24 days of continuous detection twice a day. The detection results showed that the H2S was 0.04-0.08 mg / m 3 , the NH3 was 0.9-1.6 mg / m 3 , and the odor concentration was 6-15, which could stably reach the second level of the factory boundary in the “Odor Pollutant Emission Standard” (GB14554-93).

[0138] Example 3

[0139] A preparation method of a combined filler for biological deodorization (containing activated carbon multi-strain filler):

[0140] Preparation of V filler:

[0141] Pseudomonas phenazinium, Pseudomonas stutzeri, yeast, Desulfobulbus phenoxidiants, Alcaligenes sp. were respectively cultured in a formula of peptone 20 parts, beef extract 7 parts, sodium chloride 4 parts, water 70 parts to the late logarithmic phase, and then the five bacterial suspensions were mixed in equal amounts, and 125 parts of polyvinyl alcohol were mixed in as the embedding agent for the V filler bacterial group. After mixing, the bacterial suspension was embedded and protected for use;

[0142] 70 parts of polypropylene (PP), 8 parts of powdered activated carbon, and 8 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and the mixture was heated to 220±10℃ in a foaming machine to make the PP mixture expand and form a porous structure. The preliminarily foamed PP mixture was mixed with 15 parts of the completed V filler bacterial group by immersion, and then the mixture was placed in a mold and pressed to form a shape. During the pressing process, the V filler bacterial group embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and firmly combined. After the pressing was completed, the V filler was obtained, which was cut into blocks with a size of 2×2 mm for use.

[0143] H filler preparation:

[0144] Rhodopseudomonas palustris, mud green bacteria, chromogenic bacteria, and sulfur-producing bacteria were respectively cultured in a formula of yeast extract 8 parts, ammonium chloride 2 parts, dipotassium hydrogen phosphate 2 parts, and water 64 parts to the late logarithmic phase. The four bacterial suspensions were mixed in equal amounts, and 120 parts of polyvinyl alcohol and 80 parts of sodium bicarbonate were mixed in. Sodium bicarbonate was used as a protective agent for the H filler. After mixing, the bacterial suspension was embedded and protected for use.

[0145] 60 parts of polypropylene (PP), 10 parts of powdered activated carbon, and 10 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and the mixture was heated to 220±10℃ in a foaming machine to make the PP mixture expand and form a porous structure. The preliminarily foamed PP mixture was mixed with 20 parts of the completed H filler bacterial group by immersion, and then the mixture was placed in a mold and pressed to form a shape. During the pressing process, the H filler bacterial group embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and firmly combined. After the pressing was completed, the H filler was obtained, which was cut into blocks with a size of 3×3 mm for use.

[0146] N filler preparation:

[0147] Nitrifying bacteria were cultured in a medium of peptone 20 parts, beef extract 7 parts, sodium chloride 4 parts, and water 70 parts to the late logarithmic phase, and then 25 parts of polyvinyl alcohol were mixed in. After mixing, the bacterial suspension was embedded and protected for use.

[0148] 60 parts of polypropylene (PP), 10 parts of powder activated carbon, 10 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and heating treatment was carried out in a foaming machine to 220±10℃, so that the PP mixture was expanded to form a porous structure. The preliminarily foamed PP mixture was placed into 20 parts of immobilized Q filler bacterial groups for soaking and mixing uniformly, and then was placed into a mold for compression molding. During the compression process, the Q filler bacterial groups embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and firmly combined. After the compression was completed, the Q filler was prepared, which was cut into 2x2mm block size for use.

[0149] Q filler preparation:

[0150] Bacillus, Lactobacillus plantarum, beer yeast and Nocardia were respectively cultured in a formula of 15 parts of proteose peptone, 30 parts of beef extract, 10 parts of sodium chloride and 60 parts of water to the late logarithmic phase of the bacterial strains, and then the three bacterial suspensions were mixed in equal amounts. At the same time, 120 parts of polyvinyl alcohol were mixed, and the cultured bacterial suspension was embedded and protected for use.

[0151] 60 parts of polypropylene (PP), 10 parts of powder activated carbon, 10 parts of sodium alginate were mixed uniformly, a commercially available PP foaming agent was added, and heating treatment was carried out in a foaming machine to 220±10℃, so that the PP mixture was expanded to form a porous structure. The preliminarily foamed PP mixture was placed into 20 parts of immobilized Q filler bacterial groups for soaking and mixing uniformly, and then was placed into a mold for compression molding. During the compression process, the Q filler bacterial groups embedded by polyvinyl alcohol and the PP mixture were fully crosslinked and firmly combined. After the compression was completed, the Q filler was prepared, which was cut into 2x2mm block size for use.

[0152] Combination use:

[0153] The original deodorization equipment of a sewage treatment plant used ceramic filter as filler. After the original system was operated for 2 years, the H2S was 0.56-0.68mg / m 3 , NH3 was 3.5-6.7mg / m 3 , and odor concentration was 60-72 at the monitoring point of the sewage plant, which could not stably reach the third level of the factory boundary in the "Odor Pollutant Discharge Standard" (GB14554-93). The V filler 30 parts, H filler 30 parts, N filler 20 parts and Q filler 20 parts in Example 3 were combined in turn to replace the combined filler in Example 2, and it was found that the performance of the combined filler started quickly, and the odor at the monitoring point was obviously reduced after 5 days of operation. After the odor was obviously reduced for 5 days, the odor detection was carried out at the monitoring point for 2 times a day for 25 consecutive days. After detection, the H2S was 0.05-0.08mg / m 3 , NH3 was 0.8-1.3mg / m 3The odor concentration is 8-17, and the factory boundary secondary standard in the "odor pollutant discharge standard" (GB14554-93) can be stably reached.

[0154] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, but not limited to them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for preparing a combined filler for biological deodorization, characterized in that, The combination filler comprises V filler, H filler, N filler and Q filler; The V filler loaded bacterial community comprises at least one of Pseudomonas phenazinium, Pseudomonas stutzeri, yeast, Desulfobacterium phenazinium or Alcaligenes faecalis; The H filler loaded bacterial community comprises at least one of Rhodopseudomonas palustris, Chloronema sp., Chromatium sp. or Sulfolobus acidocaldarius; The N filler loaded bacterial community comprises nitrifying bacteria; The Q filler loaded bacterial community comprises at least one of Bacillus sp., Lactobacillus plantarum, Saccharomyces cerevisiae or Nocardia sp.; The preparation method of the V filler, H filler, N filler or Q filler comprises the following steps: a. embedding bacterial suspension with polyvinyl alcohol as embedding agent to obtain polyvinyl alcohol embedded bacterial suspension; b. mixing polypropylene, activated carbon and sodium alginate, then adding polypropylene foaming agent for foaming treatment to obtain foaming product; c. mixing the foaming product obtained in step b and the polyvinyl alcohol embedded bacterial suspension prepared in step a, then performing compression molding and crosslinking in a mold to obtain V filler, H filler, N filler or Q filler; The V filler, H filler, N filler and Q filler each independently comprises polypropylene 50-70 parts, activated carbon 5-10 parts, sodium alginate 5-10 parts and polyvinyl alcohol embedded bacterial suspension 10-20 parts by mass fraction.

2. The production method according to claim 1, characterized by, In step a, the step of embedding bacterial suspension with polyvinyl alcohol as embedding agent is as follows: After the bacteria are inoculated in the culture solution and cultured to the late logarithmic phase, the polyvinyl alcohol embedded bacterial suspension is obtained by mixing with polyvinyl alcohol.

3. The preparation method according to claim 2, characterized in that, The culture solution of the V filler bacterial suspension comprises peptone, beef extract, sodium chloride and water; In the V filler, the raw materials of the polyvinyl alcohol embedded bacterial suspension comprise polyvinyl alcohol 15-25 parts, peptone 10-20 parts, beef extract 2-7 parts, sodium chloride 3-5 parts and water 50-70 parts by mass fraction.

4. The production method according to claim 2, characterized by, The culture solution of the H filler bacterial suspension comprises yeast extract, ammonium chloride, dipotassium hydrogen phosphate, sodium bicarbonate and water; In the H filler, the raw materials of the polyvinyl alcohol embedded bacterial suspension comprise polyvinyl alcohol 20-30 parts, yeast extract 5-10 parts, ammonium chloride 0.5-2 parts, dipotassium hydrogen phosphate 0.5-2 parts and water 36-64 parts by mass fraction.

5. The preparation method according to claim 2, characterized in that, The culture solution of the N filler bacterial suspension comprises peptone, beef extract, sodium chloride and water; In the N filler, the raw materials of the polyvinyl alcohol embedded bacterial suspension comprise polyvinyl alcohol 15-25 parts, peptone 10-20 parts, beef extract 2-7 parts, sodium chloride 3-5 parts and water 50-70 parts by mass fraction.

6. The preparation method according to claim 2, characterized in that, The culture solution of the Q filler bacterial suspension comprises peptone, beef extract, sodium chloride and water; In the Q filler, the raw materials of the polyvinyl alcohol embedded bacterial suspension comprise polyvinyl alcohol 20-30 parts, peptone 5-15 parts, beef extract 10-30 parts, sodium chloride 5-10 parts and water 15-60 parts by mass fraction.

7. The preparation method according to claim 1, characterized in that, The combination filler comprises V filler 20-40 parts, H filler 15-35 parts, N filler 10-30 parts and Q filler 5-25 parts by mass fraction.

8. The method of claim 1, wherein, The V filler loaded bacterial community is Pseudomonas phenazinium, Pseudomonas stutzeri, Saccharomycetes, Desulfobacterium phenoxodum and Alcaligenes; The H filler loaded bacterial community is Rhodopseudomonas palustris, Chlorobium limicola, Chromatium and Sulfolobus acidocaldarius; The Q filler loaded bacterial community is Bacillus, Lactobacillus plantarum, Saccharomyces cerevisiae and Nocardia.

9. A combined filler for biological deodorization, characterized in that, The preparation method of any one of claims 1-8 is adopted to prepare.

10. The application of the combined filler of claim 9 in sewage treatment.