Platelet sorting device and sorting method
By designing a platelet sorting device, using the combination technology of microflower and acoustic transducer, efficient separation of platelets is achieved, and the problems of low separation efficiency and negative impact on platelet function in the prior art are solved.
Patent Information
- Application Number
- CN202510022850.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-07
- Publication Date
- 2025-03-28
AI Technical Summary
In the prior art, the separation efficiency of platelets is low and has a negative impact on platelet function and integrity, making it difficult to effectively separate platelets from submicron-level particles.
A platelet sorting device is designed, and by using the connected first and second microflowers and corresponding transducers, the standing wave field uniformly distributed in the pressure node line is used to control the particles of micron and submicron particle size to generate lateral displacement during the flow process, thereby achieving efficient separation of platelets.
It improves the separation efficiency and purity of platelets, significantly reduces sorting time, is easy to operate, is cheap to the instrument and has high separation accuracy.
Smart Images
Figure CN119709365A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of microfluidics, and in particular to a platelet sorting device and a platelet sorting method. Background Art
[0002] Platelets are an important component of the blood. They not only assist in hemostasis, coagulation, vasoconstriction and repair of damaged blood vessels, but can also accelerate the recovery process of human bones and soft tissues. Platelet extraction is of great significance in medical treatment and can help patients restore normal hemostasis and coagulation functions, thus ensuring their health.
[0003] Blood contains many particles of different sizes, such as red blood cells and white blood cells with a particle size of micrometer level (particle size between 1 and 100 micrometers), and some chylomicrons and protein aggregates with a particle size of submicrometer level (particle size between 100 nanometers (nm) and 1 micrometer (μm)). In the prior art, high-speed centrifugation or microporous membrane filtration is usually used to sort and enrich platelets in blood. Taking high-speed centrifugation as an example, high-speed centrifugation uses a continuous centrifugation device to separate the patient's blood into different parts according to the difference in density of each component in the blood (red blood cells> white blood cells> platelets> plasma); however, this centrifugation separation method takes a long time (several hours), and high-speed centrifugation is very likely to have a negative impact on the function and integrity of platelets; in addition, the particle size of platelets is about 2-4 micrometers, which is similar to the particle size of submicrometer particles. Traditional methods can usually only separate larger particle sizes in blood (such as red blood cells and white blood cells), and it is not easy to separate platelets from submicrometer particles, which affects the effect of platelet sorting. Summary of the invention
[0004] In order to improve the platelet separation efficiency and the platelet separation effect at the same time, a platelet separation device and a platelet separation method are provided.
[0005] In order to achieve the above-mentioned purpose of the present invention, the present invention provides a platelet separation device.
[0006] The present invention provides a platelet sorting device, comprising a first microchannel and a second microchannel connected to each other, wherein the first microchannel comprises a first liquid inlet section and a first sorting section, one end of the first sorting section is connected to the first liquid inlet section, and the other end is connected to a first output channel and a second output channel; a first transducer is provided on the outer wall of the first sorting section, and the first transducer is used to sort particles with a micron-sized particle size;
[0007] The second microchannel includes a second sorting section, one end of which is connected to the first output channel, and the other end is connected to the third output channel and the fourth output channel. The outer wall of the second sorting section is provided with a second transducer, and the second transducer is used to sort particles with submicron particle size.
[0008] Optionally, a first wedge-shaped block is provided on the outer wall of the first selection section, the sound wave emitting end of the first transducer abuts against the side wall of the first wedge-shaped block away from the first selection section, and an acute angle is formed between the central axis of the first selection section and the plane where the sound wave emitting end of the first transducer is located;
[0009] A second wedge block is arranged on the outer wall of the second sorting section, the sound wave emitting end of the second transducer abuts against the side wall of the second wedge block away from the second sorting section, and an acute angle is formed between the central axis of the second sorting section and the plane where the sound wave emitting end of the second transducer is located.
[0010] Optionally, the angle between the central axis of the first selection segment and the plane where the sound wave emitting end of the first transducer is located is α1, the angle between the central axis of the second selection segment and the plane where the sound wave emitting end of the second transducer is located is α2, the value range of the angle α1 is 15°<α1<21°, and the value range of the angle α2 is 15°<α2<21°.
[0011] Optionally, the first sorting section includes a first fluid channel and a second fluid channel, the first fluid channel is connected to the first output channel, and an extension direction of the first fluid channel is arranged at an acute angle to a plane where the sound wave emitting end of the first transducer is located;
[0012] The second fluid channel is communicated with the second output channel, and an extension direction of the second fluid channel is parallel to a plane where the sound wave emitting end of the first transducer is located.
[0013] Optionally, the first fluid channel includes at least one first target channel. When there are multiple first target channels, all of the multiple first target channels are connected to the first liquid inlet section, and the first output channel is connected to one of the first target channels.
[0014] Optionally, the second sorting section includes a third fluid channel and a fourth fluid channel, the third fluid channel is connected to the third output channel, and the extension direction of the third fluid channel is parallel to the plane where the sound wave emitting end of the second transducer is located;
[0015] The fourth fluid channel is communicated with the fourth output channel, and an extension direction of the fourth fluid channel is arranged at an acute angle to a plane where the sound wave emitting end of the second transducer is located.
[0016] Optionally, the first transducer and the second transducer are traveling wave acoustic transducers.
[0017] Optionally, the force exerted on the particle by the first transducer or the second transducer is
[0018]
[0019] Where p0 is the sound pressure amplitude (Pa), Vp is the particle volume (m 3 ), β w is the liquid compressibility coefficient (Pa -1 ), λ is the signal wavelength (m), is the acoustic contrast coefficient, y is the distance between the particle and the node of the standing wave (m);
[0020] The expression of acoustic contrast coefficient is:
[0021]
[0022] Among them, β p is the particle compression coefficient (Pa -1 );ρ p is the particle density (kg / m 3 );ρ w is the density of water (kg / m 3 ).
[0023] In order to solve the above problems, the present invention further provides a platelet separation method, which is applied to the above platelet separation device, and the method comprises:
[0024] The state of the first liquid inlet section is monitored, and the first transducer is turned on when it is detected that the solution to be sorted is injected into the first liquid inlet section. After the first transducer is turned on, a first standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles of different particle sizes during the flow process, and the particles corresponding to the platelet particle size or particles smaller than the platelet particle size are introduced into the first output channel;
[0025] The liquid entering the first output channel is recorded as the first sorting solution. When the first sorting solution is monitored to be injected into the first liquid inlet section, the second transducer is turned on. After the second transducer is turned on, a second standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles with micron and submicron sizes during the flow process. Particles with micron sizes and particles with submicron sizes enter different output channels respectively.
[0026] Optionally, the platelet separation device further comprises a vortex mixer for mixing the solution to be separated, and the method further comprises:
[0027] The solution to be sorted is uniformly mixed by a vortex mixer, and then the solution to be sorted is injected into the first liquid inlet section.
[0028] In summary, this application includes the following beneficial technical effects:
[0029] The first transducer can generate a standing wave field with uniformly distributed pressure node lines to make the particles to be sorted move along the inclined direction of the standing wave field, so as to control the particles with micron size to enter the first output channel or the second output channel, and separate the particles with micron size such as red blood cells and white blood cells from the platelet particles in the whole blood solution to be sorted; then the second transducer is used to perform secondary sorting on the treated platelet solution; the particles with submicron size in the treated platelet solution are separated from the platelet particles to obtain the final platelet separation liquid. The whole sorting process is easy to operate, the equipment is cheap, the separation accuracy is high, and the target particles in the separated target solution are of high purity. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] Figure 1 A schematic diagram of the structure of a platelet separation device provided in one embodiment of the present invention;
[0031] Figure 2 It is a schematic diagram of particle distribution of the solution to be sorted when it just enters the first sorting section;
[0032] Figure 3 It is a schematic diagram of particle distribution of the solution to be sorted after the first transducer sorts particles of different particle sizes;
[0033] Figure 4 This is a schematic diagram of particle distribution of the first sorting solution just entering the second sorting section;
[0034] Figure 5 This is a schematic diagram of particle distribution after the first sorting solution is sorted by the second transducer to sort particles of different particle sizes.
[0035] Figure numerals: 1. first microfluidic channel; 11. first liquid inlet section; 12. first sorting section; 13. first output channel; 14. second output channel; 2. second microfluidic channel; 21. second sorting section; 22. third output channel; 23. fourth output channel; 3. first transducer; 4. second transducer; 5. first wedge block; 6. second wedge block.
[0036] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION
[0037] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.
[0038] In the description of the present invention, it is necessary to understand that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0039] In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a mechanical connection or an electrical connection, or it can be the internal connection between two components. It can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances.
[0040] Reference Figure 1 FIG. 1 is a flow chart of a platelet separation device provided in one embodiment of the present invention. In this embodiment, the platelet sorting device includes a first microchannel 1 and a second microchannel 2 which are connected to each other, wherein the first microchannel 1 includes a first liquid inlet section 11 and a first sorting section 12, one end of the first sorting section 12 is connected to the first liquid inlet section 11, and the other end is connected to a first output channel 13 and a second output channel 14; a first transducer 3 is provided on the outer wall of the first sorting section 12, and the first transducer 3 can sort particles with a micron-sized particle size; the sound wave emitting end of the first transducer 3 is inclined to the outer wall of the first sorting section 12, and the first transducer 3 is turned on after the whole blood solution to be sorted is input into the first liquid inlet section 11, and the first transducer 3 can generate a standing wave field with a uniformly distributed pressure node line to make the particles to be sorted move along the inclined direction of the standing wave field, so as to control the particles with a micron-sized particle size to enter the first output channel 13 or the second output channel 14, and separate the particles with a micron-sized particle size such as red blood cells and white blood cells in the whole blood solution to be sorted from the platelet particles.
[0041] In this embodiment, particles with smaller particle sizes are discharged from the first output channel 13, and particles with larger particle sizes are discharged from the second output channel 14; the second microchannel 2 includes a second sorting section 21, one end of the second sorting section 21 is connected to the first output channel 13, and the other end is connected to the third output channel 22 and the fourth output channel 23. The outer wall of the second sorting section 21 is provided with a second transducer 4, and the second transducer 4 is used to sort particles with submicron particle sizes. The sound wave emitting end of the first transducer 3 is inclined to the outer wall of the first sorting section 12.
[0042] The liquid entering the first output channel 13 is recorded as the first sorting solution. After the first sorting solution is input into the first output channel 13, the second transducer 4 is turned on. After the second transducer 4 is turned on, it emits sound waves. The generated sound waves are reflected by the wall of the second sorting section 21 to form a second standing wave field with uniformly distributed pressure node lines, so that particles of different particle sizes produce lateral displacement during the flow process, so as to control particles of different particle sizes to enter the third output channel 22 or the fourth output channel 23, and separate the submicron particle size particles from the platelet particles in the first sorting solution to obtain the final platelet separation liquid. The whole sorting process is easy to operate, the equipment is cheap, the separation accuracy is high, and the purity of the target particles in the separated target solution is high. The time unit for processing the whole blood solution using micro-control flow technology is seconds or minutes. Compared with the traditional method that takes several hours to separate platelets, it can significantly reduce the sorting time and improve the sorting efficiency.
[0043] In a preferred implementation of this embodiment, the first transducer 3 and the second transducer 4 are acoustic traveling wave transducers.
[0044] A first wedge block 5 is provided on the outer wall of the first selection section 12, and the cross-sectional profile of the first wedge block 5 is a right triangle. The sound wave emitting end of the first transducer 3 abuts against the side wall of the first wedge block 5 away from the first selection section 12, and the central axis of the first selection section 12 and the plane where the sound wave emitting end of the first transducer 3 is located are arranged at an acute angle; the angle between the central axis of the first selection section 12 and the plane where the sound wave emitting end of the first transducer 3 is located is α1. In the present embodiment, the value range of the angle α1 is 15°<α1<21°.
[0045] The first transducer 3 can generate sound waves after being turned on, and a standing wave field with uniformly distributed pressure node lines formed by reflection from the opposite wall causes the particles to be sorted to move along the inclined direction of the standing wave field. Particles of different particle sizes move away from the first transducer 3 at different speeds after being acted upon by external forces. Specifically, particles with larger particle sizes move away from the first transducer 3 at a speed greater than that of particles with smaller particle sizes, thereby achieving separation of particles of different particle sizes.
[0046] It is worth noting that in this embodiment, under the action of the standing wave field, the force of the particle on the acoustic field node or anti-node depends on the acoustic contrast of the particle material relative to the medium, and the force on particles of different particle sizes is as follows:
[0047]
[0048] in, is the acoustic contrast coefficient, β p is the particle compression coefficient (Pa -1 ), β w is the liquid compressibility coefficient (Pa-1 ), ρ p is the particle density (kg / m 3 ), ρ w is the density of water (kg / m 3 ).
[0049] When the acoustic contrast factor is positive, the particle will move toward the node, and the force acting on the particle in the acoustic field is:
[0050]
[0051] Where p0 is the sound pressure amplitude (Pa), V p is the particle volume (m 3 ), λ is the signal wavelength (m), and y is the distance between the particle and the node of the standing wave (m). When the particle and the liquid near it produce relative displacement, they will be subject to viscous resistance F which is proportional to the relative displacement speed. d The resistance value is:
[0052] F d =-6πη w dv
[0053] Among them, F d is the viscous resistance of the particle (N); d is the particle diameter (m); v is the relative velocity between the particle and the liquid (m / s). r >F d The force exerted by the acoustic field controls the movement of particles, and the particles approach the acoustic nodes. It can be seen that the movement of the particles in the first sorting channel is affected by factors such as particle diameter, sound pressure, liquid viscosity, and frequency. In order to improve the sorting effect, before the solution to be sorted is input into the first sorting section 12, the solution to be sorted can be placed in a centrifuge tube, and then the centrifuge tube can be placed on a vortex mixer for oscillation mixing to make the solution evenly mixed.
[0054] In this embodiment, the first sorting section 12 includes a first fluid channel close to the first transducer 3 and a second fluid channel away from the first transducer 3. The first fluid channel is connected to the first output channel 13, and the second fluid channel is connected to the second output channel 14. The side wall of the first sorting section 12 away from the first transducer 3 is inclined in the direction away from the central axis of the first sorting section 12, and the inner diameter of the channel of the first sorting section 12 close to the first liquid inlet section 11 is smaller than the inner diameter of the channel of the first sorting section 12 away from the first liquid inlet section 11. The extension direction of the second fluid channel is parallel to the plane where the sound wave emitting end of the first transducer 3 is located, thereby increasing the lateral movement space of particles with larger micron-sized particles in the whole blood solution to be sorted inside the first sorting section 12, thereby improving the sorting effect of micron-sized particles of different particle sizes.
[0055] The extension direction of the first fluid channel is set at an acute angle to the plane where the sound wave emitting end of the first transducer 3 is located, and the extension direction of the second fluid channel is parallel to the plane where the sound wave emitting end of the first transducer 3 is located; in this embodiment, after the first transducer 3 is turned on, the speed at which the particles with larger particle size move away from the first transducer 3 is greater than the speed at which the particles with smaller particle size move away from the first transducer 3; after the first transducer 3 is turned on, the micron-sized particles with larger particle size (such as red blood cells, white blood cells or protein particles with a particle size larger than platelets) in the whole blood solution to be sorted move faster in the direction away from the first transducer 3 and flow out from the second output channel 14; the micron-sized particles and submicron-sized particles (such as platelets or particles with a particle size at the submicron level) with smaller particle size move slower in the direction away from the first transducer 3 and flow out from the first output channel 13.
[0056] The first fluid channel includes at least one first target channel. In a preferred implementation of the present embodiment, a plurality of first target channels are provided, and the plurality of first target channels are all connected to the first liquid inlet section 11, and the first output channel 13 is connected to one of the first target channels. Since a plurality of first target channels are provided in the solution, the first transducer 3 can drive particles of different sizes to move laterally at different speeds after being turned on, so that particles of different sizes can be exported from different first target channels, so that the sorting device can separate particles of multiple sizes at one time, thereby improving the sorting efficiency of particles of different sizes in the solution.
[0057] A second wedge block 6 is provided on the outer wall of the second selecting section 21, the sound wave emitting end of the second transducer 4 abuts against the side wall of the second wedge block 6 away from the second selecting section 21, and the central axis of the second selecting section 21 and the plane where the sound wave emitting end of the second transducer 4 is located are arranged at an acute angle; the angle between the central axis of the second selecting section 21 and the plane where the sound wave emitting end of the second transducer 4 is located is α2, and the value range of the angle α2 is 15°<α2<21°.
[0058] The second sorting section 21 includes a third fluid channel and a fourth fluid channel, the third fluid channel is connected to the third output channel 22, and the fourth fluid channel is connected to the fourth output channel 23. The side wall of the second sorting section 21 away from the second transducer 4 is inclined in the direction away from the central axis of the second sorting section 21, and the inner diameter of the channel of the second sorting section 21 close to the first output channel 13 is smaller than the inner diameter of the channel of the second sorting section 21 away from the first output channel 13. The extension direction of the third fluid channel is parallel to the plane where the sound wave emitting end of the second transducer 4 is located; the fourth fluid channel is connected to the fourth output channel 23, and the extension direction of the fourth fluid channel is set at an acute angle to the plane where the sound wave emitting end of the second transducer 4 is located.
[0059] The calculation formulas of the force exerted on the particle by the second transducer 4 and the first transducer 3 are both
[0060] The only difference is in the values of the sound pressure amplitude p0 and the wavelength λ, which will not be described here for the sake of brevity.
[0061] After the second transducer 4 is turned on, the micron-sized particles with larger particle sizes (such as platelets) in the first sorting solution move faster in the direction away from the second transducer 4 and flow out from the third output channel 22; the submicron-sized particles with smaller particle sizes (such as submicron-sized chylomicrons and protein aggregate particles) move slower in the direction away from the second transducer 4 and flow out from the fourth output channel 23; the submicron-sized particles in the first sorting solution are separated from the platelet particles to obtain a platelet separation solution with higher purity.
[0062] The first transducer 3 and the second transducer 4 are traveling acoustic wave transducers (BAW transducers). Exemplarily, in this embodiment, the first transducer 3 uses a 2 MHz BAW transducer, and the second transducer 4 uses a 4 MHz BAW transducer. Microchannels are etched on a glass substrate using a photolithography machine.
[0063] In order to verify the separation effect of the platelet separation device of the present application on platelet particles, the present application selected a microsphere sample solution obtained by mixing microspheres with a diameter of 10 microns, microspheres with a diameter of 3 microns and microspheres with a diameter of 1 micron to verify the separation effect of the platelet separation device on 3 micron microspheres (the particle size of platelet particles is 2-4 microns):
[0064] First, use a pipette to take 100 μl (microliter) of the microsphere sample solution into a 1.5 ml centrifuge tube, then take 900 μl (microliter) of a 0.1% Tween 20 aqueous solution into the centrifuge tube, and then place the centrifuge tube on a vortex mixer for oscillation mixing to ensure that the solution is evenly mixed and the polystyrene microspheres are evenly dispersed.
[0065] The solution prepared above is introduced into the first liquid inlet section 11, and the solution channel flow rate is 6e -7 m 3 / s, reference Figure 2 and Figure 3 After the first transducer 3 is turned on, the microspheres with a particle size greater than 10 microns enter the second output channel 14 after being sorted by the acoustic body wave standing wave field channel, and the microspheres with a diameter of 3 microns and the microspheres with a diameter of 1 micron do not migrate and directly enter the first output channel 13;
[0066] refer to Figure 4 and Figure 5After the second transducer 4 is turned on, the microspheres with a particle size of 3 microns enter the third output channel 22 after being sorted by the acoustic body wave standing wave field channel; the microspheres with a diameter of 1 micron do not migrate and directly enter the fourth output channel 23; it can be seen that the platelet sorting device of the present application can effectively sort platelets.
[0067] Based on the above platelet separation device, an embodiment of the present invention further provides a platelet separation method, comprising:
[0068] S1, monitoring the state of the first liquid inlet section 11, and starting the first transducer 3 when it is detected that the solution to be sorted is injected into the first liquid inlet section 11. After the first transducer 3 is started, a first standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles of different particle sizes during the flow process, and the particles corresponding to the platelet particle size or particles smaller than the platelet particle size are introduced into the first output channel 13,
[0069] S2. The liquid entering the first output channel 13 is recorded as the first sorting solution. When the first sorting solution is monitored to be injected into the first liquid inlet section 11, the second transducer 4 is turned on. After the second transducer 4 is turned on, a second standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles with micron and submicron particle sizes during the flow process, so that particles with micron particle sizes and particles with submicron particle sizes enter different output channels respectively.
[0070] In a preferred embodiment of this embodiment, the platelet sorting method further comprises:
[0071] S10, uniformly mixing the solution to be sorted by a vortex mixer.
[0072] After the solution to be sorted is centrifugally mixed, particles of different particle sizes in the solution are evenly dispersed, so that the particles can achieve better sorting effects in subsequent sorting steps.
[0073] After the particles in the solution are sorted, the first microchannel 1 and the second microchannel 2 are cleaned with a cleaning solution to facilitate the subsequent use of the platelet sorting device.
[0074] It should be understood that the embodiments are for illustrative purposes only and are not limited to this structure in the scope of the patent application. In the description of this specification, the description of reference terms "one embodiment", "some embodiments", "example", "specific example", "an implementation", "a preferred implementation" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0075] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.
Claims
1. A platelet separation device, characterized in that: The invention comprises a first microfluidic channel (1) and a second microfluidic channel (2) which are connected to each other. The first microfluidic channel (1) comprises a first liquid inlet section (11) and a first sorting section (12). One end of the first sorting section (12) is connected to the first liquid inlet section (11), and the other end is connected to a first output channel (13) and a second output channel (14). A first transducer (3) is arranged on the outer wall of the first sorting section (12), and the first transducer (3) is used to sort particles with a micron-sized particle size. The second microfluidic channel (2) comprises a second sorting section (21), one end of the second sorting section (21) is connected to the first output channel (13), and the other end is connected to a third output channel (22) and a fourth output channel (23), and a second transducer (4) is arranged on the outer wall of the second sorting section (21), and the second transducer (4) is used to sort particles with a submicron particle size.
2. The platelet separation device according to claim 1, characterized in that: A first wedge-shaped block (5) is arranged on the outer wall of the first selection section (12); the sound wave emitting end of the first transducer (3) abuts against the side wall of the first wedge-shaped block (5) away from the first selection section (12); and the central axis of the first selection section (12) and the plane where the sound wave emitting end of the first transducer (3) is located form an acute angle; A second wedge-shaped block (6) is arranged on the outer wall of the second sorting section (21); the sound wave emitting end of the second transducer (4) abuts against the side wall of the second wedge-shaped block (6) away from the second sorting section (21); and an acute angle is formed between the central axis of the second sorting section (21) and the plane where the sound wave emitting end of the second transducer (4) is located.
3. The platelet separation device according to claim 2, characterized in that: The angle between the central axis of the first selection section (12) and the plane where the sound wave emitting end of the first transducer (3) is located is α1, and the angle between the central axis of the second selection section (21) and the plane where the sound wave emitting end of the second transducer (4) is located is α2. The value range of the angle α1 is 15°<α1<21°, and the value range of the angle α2 is 15°<α2<21°.
4. The platelet separation device according to claim 2 or 3, characterized in that: The first sorting section (12) comprises a first fluid channel and a second fluid channel, the first fluid channel is connected to the first output channel (13), and the extension direction of the first fluid channel is arranged at an acute angle to the plane where the sound wave emitting end of the first transducer (3) is located; The second fluid channel is in communication with the second output channel (14), and the extension direction of the second fluid channel is parallel to the plane where the sound wave emitting end of the first transducer (3) is located.
5. The platelet separation device according to claim 4, characterized in that: The first fluid channel comprises at least one first target channel. When there are multiple first target channels, all of the multiple first target channels are connected to the first liquid inlet section (11), and the first output channel (13) is connected to one of the first target channels.
6. The platelet separation device according to claim 2 or 3, characterized in that: The second sorting section (21) comprises a third fluid channel and a fourth fluid channel, the third fluid channel is connected to the third output channel (22), and the extension direction of the third fluid channel is parallel to the plane where the sound wave emitting end of the second transducer (4) is located; The fourth fluid channel is in communication with the fourth output channel (23), and the extension direction of the fourth fluid channel is arranged at an acute angle to the plane where the sound wave emitting end of the second transducer (4) is located.
7. The platelet separation device according to claim 1, 2, 3 or 5, characterized in that: The first transducer (3) and the second transducer (4) are acoustic traveling wave transducers.
8. The platelet separation device according to claim 7, characterized in that: The force exerted on the particle by the first transducer (3) or the second transducer (4) is Where p0 is the sound pressure amplitude (Pa), V p is the particle volume (m 3 ), β w is the liquid compressibility coefficient (Pa -1 ), λ is the signal wavelength (m), is the acoustic contrast coefficient, y is the distance between the particle and the node of the standing wave (m); The expression of acoustic contrast coefficient is: Among them, β p is the particle compression coefficient (Pa -1 );ρ p is the particle density (kg / m 3 );ρ w is the density of water (kg / m 3 ).
9. A platelet separation method, applied to the platelet separation device according to any one of claims 1 to 9, characterized in that: The method comprises: The state of the first liquid inlet section (11) is monitored, and the first transducer (3) is turned on when it is detected that the solution to be sorted is injected into the first liquid inlet section (11). After the first transducer (3) is turned on, a first standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles of different particle sizes during the flow process, and to guide particles corresponding to or smaller than the platelet particle size into the first output channel (13); The liquid entering the first output channel (13) is recorded as the first sorting solution. When the first sorting solution is monitored to be injected into the first liquid inlet section (11), the second transducer (4) is turned on. After the second transducer (4) is turned on, a second standing wave field with uniformly distributed pressure node lines is generated to control the lateral displacement of particles with micron and submicron particle sizes during the flow process. The particles with micron particle sizes and the particles with submicron particle sizes enter different output channels respectively.
10. The platelet separation method according to claim 9, characterized in that: The platelet separation device further comprises a vortex mixer for mixing the solution to be separated, and the method further comprises: The solution to be sorted is uniformly mixed by a vortex mixer, and then the solution to be sorted is injected into the first liquid inlet section (11).