Sampling device for bacteria detection

Abstract

The invention relates to the field of bacterial sampling, in particular to a sampling device for bacterial detection, which comprises a test tube seat, a test tube is detachably connected onto the test tube seat, a support is arranged on the test tube seat, a paper sample frame is arranged on the support, the paper sample frame is positioned on one side, far away from the test tube seat, of the test tube, and an alignment opening is formed in the paper sample frame. The paper sample frame is used for placing a paper sample carrying a sample, and the sample on the paper sample is positioned in the alignment opening; a sampling assembly is fixedly connected to the bracket and comprises a laser transmitter, a first preserving fluid releaser and a controller; the controller is used for controlling the laser transmitter to burn a through hole in the paper sample on the periphery of the sample after the paper sample is placed, and then controlling the first preserving fluid releaser to release the preserving fluid, so that the preserving fluid washes the sample and enters the test tube through the through hole and the alignment opening. By adopting the technical scheme provided by the invention, the through holes can be burnt on the paper sample, so that the sample flows into the test tube under the washing of the preserving fluid, the sample does not need to be contacted, and the use of consumables and the disinfection frequency are reduced.

Description

Technical Field

[0001] The invention relates to the field of bacteria sampling, and in particular to a sampling device for bacteria detection. Background Art

[0002] Enterococci are a type of bacteria widely distributed in nature, often living in the intestines of humans and animals and the female reproductive tract. They are Gram-positive cocci, arranged in single, double or short chains, and arranged in oval and chain shapes in liquid culture. Enterococci have no spores, no capsules, most have no flagella (some species have sparse flagella), are facultative anaerobic, have an optimal growth temperature of 35°C, can grow at both 10°C and 45°C, and have high nutritional requirements. In the prior art, Enterococcus faecalis is often collected for testing. The specific steps are to provide the patient with a paper sample of wiping feces, collect the sample on the paper sample with a cotton swab, and then insert the cotton swab into a test tube containing a preservation solution, repeatedly stir to fully dissolve the sample, and then seal the test tube.

[0003] In the prior art, for example, Patent Publication No. CN204789013U discloses a sampling rod for a feces sampling device, which improves the sample adsorption capacity by adding scales to the sampling head. Patent Publication No. CN219590051U discloses a liquid addition and sampling needle automatic adjustment device for feces detection, which completes sampling and sample injection by program-controlled needle body. Patent Publication No. CN112557162A discloses a feces sampling device, which samples through a sampling rod and probes into the cavity for mixing. However, whether it is the sampling head, needle body or sampling rod, it will contact the sample and will inevitably adhere to the sample. When used for continuous sampling, it still needs to be replaced or disinfected at the sampling interval. Secondly, CN112557162A has a mixing function, but it relies on a liquid transmission line, making it more difficult to replace or disinfect, and cleaning after use is also more troublesome. Summary of the invention

[0004] To solve the above problems, the present invention provides a sampling device for bacteria detection, which can burn through holes on paper samples so that the samples flow into test tubes under the flushing of preservation fluid, thereby eliminating the need to contact the samples, reducing the use of consumables and the number of disinfection times.

[0005] In order to achieve the above-mentioned purpose, the technical scheme of the present invention is as follows: a sampling device for bacteria detection, comprising a test tube holder, a test tube is detachably connected to the test tube holder, a bracket is fixedly connected to the test tube holder, a paper sample holder is fixedly connected to the bracket, the paper sample holder is located on a side of the test tube away from the test tube holder, an alignment port is provided on the paper sample holder, the alignment port is aligned with the opening of the test tube, the paper sample holder is used to place a paper sample carrying a sample and the sample on the paper sample is located in the alignment port;

[0006] A sampling assembly is fixedly connected to the bracket, and the sampling assembly includes a laser emitter, a first preservation liquid releaser and a controller;

[0007] The laser transmitter is used for transmitting laser for burning the paper sample;

[0008] The first preservation solution releaser is used for storing and releasing the preservation solution onto the paper sample;

[0009] The controller is used to control the laser emitter to burn a through hole on the paper sample outside the sample after placing the paper sample, and then control the first preservation liquid releaser to release the preservation liquid, so that the preservation liquid flushes the sample and enters the test tube through the through hole and the alignment port.

[0010] The above scheme has the following beneficial effects:

[0011] 1. In this solution, the user unscrews the cover of the test tube and installs it on the test tube holder, then places the paper sample carrying the sample on the paper sample holder so that the sample is in the alignment port. The sampling component will automatically extract bacteria from the sample, the laser emitter will process the paper sample, burn a through hole on the paper sample, and then use the first preservation liquid releaser to flush the sample to mix the sample with the preservation liquid. The mixed preservation liquid will drip into the test tube on the test tube holder through the through hole. When the preservation liquid in the test tube is sufficient, remove the test tube and screw on the cover to complete the sampling.

[0012] 2. In this scheme, paper samples are directly used as the medium for obtaining samples and mixing samples with preservation liquid, so that in the sampling process, except for the test tubes and paper samples which are consumables themselves, no other consumables are required, and the liquid only contacts the paper samples and test tubes during the process and will not adhere to the samples. When sampling multiple times, there is no need to disinfect or replace consumables between sampling, which not only reduces the cost of consumables but also reduces the maintenance cost, making the unmanned level higher.

[0013] Furthermore, the sampling component also includes a pressing ring, and the pressing ring is provided with a first driver, and the first driver is used to make the pressing ring press the paper sample after the paper sample is placed.

[0014] Beneficial effect: The paper sample is light in weight and is easily displaced under external interference. The pressing ring can press the paper sample tightly, thereby reducing the probability of displacement of the paper sample.

[0015] Furthermore, the sampling assembly also includes an air gun, which is used to make the paper sample concave toward the test tube by blowing out air after the paper sample is placed.

[0016] Beneficial effect: The paper sample itself is easy to deform, and the air gun can construct a depression on the paper sample, so that the preservation liquid released by the first preservation liquid releaser gathers in the depression and acts on the sample.

[0017] Furthermore, the diameter of the through hole formed by burning satisfies that the outflow rate of the preservation liquid through the through hole is smaller than the release rate of the first preservation liquid releaser.

[0018] Beneficial effect: When the outflow rate is less than the release rate, the preservation liquid will not flow directly into the test tube through the through hole after being released, but will be temporarily stored in the depression to fully contact with the sample. At the same time, the preservation liquid in the depression will generate turbulence as the first preservation liquid releaser continues to release, thereby enhancing the mixing effect.

[0019] Furthermore, the test tube stand comprises a base, a guide rail is provided on the base, a test tube coordination seat is slidably connected to the guide rail, the test tube coordination seat is detachably connected to the test tube, and a second driver for driving the test tube coordination seat to slide is provided on the guide rail.

[0020] Beneficial effect: The second driver can drive the test tube coordination seat to drive the test tube to move, thereby facilitating the automatic completion of the test tube alignment and removal.

[0021] Furthermore, a waste liquid receiving tank is provided on the base, and the waste liquid receiving tank is aligned with the alignment opening.

[0022] Beneficial effect: After the test tube is taken out, the preservation liquid may continue to drip from the through hole, and the waste liquid collection tank can collect the preservation liquid to reduce pollution to the external environment.

[0023] Furthermore, an automatic capping machine is provided on one side of the guide rail away from the bracket, and the automatic capping machine is used to remove or screw on the cap of the test tube.

[0024] Beneficial effect: The automatic capping machine can automatically remove or screw on the test tube cap, further improving the degree of automation.

[0025] Furthermore, the sampling component also includes a projector, which is used to project the range of action of the preservation solution flushing on the paper sample after the paper sample is placed.

[0026] Beneficial effect: The projector can project the working range on the paper sample, which is convenient for standardizing the placement of samples on the paper sample.

[0027] Furthermore, the sampling component also includes an image capturer, which is used to obtain sample images, and a controller is used to identify the sample quantity through grayscale edge detection. The controller is preset with the corresponding test tube carrying capacity of each sample quantity. When the preservation liquid flowing into the test tube reaches the test tube carrying capacity, the second drive is controlled to make the test tube coordination seat carry the test tube to the automatic capping machine.

[0028] Beneficial effects: Although the sample can be directly sampled through the paper sample as a medium, the sample amount on the paper sample is not fixed. Some patients may have too much sample on the paper sample, which will cause the solid content of the sample in the preservation solution to be too high, which is not convenient for subsequent testing. The image capturer can detect the sample image. Since the color of the sample is significantly different from that of the paper sample, the edge of the sample can be quickly detected through grayscale to determine the amount of the sample. When the sample amount is large, the amount of preservation solution in the test tube is reduced to control the solid content in the preservation solution during subsequent testing.

[0029] Furthermore, a second preservation liquid releaser is fixedly connected to the automatic capping machine, and the controller is used to inject preservation liquid into the test tube before the automatic capping machine screws the cap on the test tube, so that the total amount of preservation liquid in the test tube reaches a preset value.

[0030] Beneficial effect: After the sample solid is restrained, the amount of preservation solution in the test tube is not equal, and the concentration difference is also large. The second preservation solution releaser can replenish the preservation solution in the test tube, so that the amount of preservation solution and the sample concentration in each test tube are within a reasonable fluctuation range.

[0031] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is an axonometric schematic diagram of an embodiment of a sampling device for bacteria detection of the present invention;

[0033] Figure 2 A schematic side view of an embodiment of a sampling device for bacteria detection according to the present invention;

[0034] Figure 3 A schematic diagram of a sampling assembly of an embodiment of a sampling device for bacteria detection according to the present invention;

[0035] Figure 4 Schematic diagram of the second preservation solution releaser of the sampling device embodiment for bacteria detection of the present invention.

[0036] The figure marks in the drawings of the specification include: 1. base; 2. guide rail; 3. test tube coordination seat; 4. first drive; 5. second drive; 6. automatic capping machine; 7. bracket; 8. alignment port; 9. laser transmitter; 10. first preservation liquid releaser; 11. air gun; 12. compression ring; 13. image capturer; 14. second preservation liquid releaser; 15. projector. DETAILED DESCRIPTION

[0037] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0039] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] The following is further described in detail through specific implementation methods:

[0041] As attached Figure 1-Figure 4 As shown: A sampling device for bacteria detection, including a test tube holder, the test tube holder including a base 1, a guide rail 2 is installed on the base 1, a test tube seat 3 is slidably connected to the guide rail 2, the test tube seat 3 is detachably connected to the test tube, a second driver 5 for driving the test tube seat 3 to slide is provided on the guide rail 2, the second driver 5 is an electric screw, an automatic capping machine 6 is installed on the side of the guide rail 2 away from the bracket 7, the automatic capping machine 6 is used to remove or screw on the cover of the test tube. The bracket 7 is screwed to the base 1, and the paper sample rack is bolted to the bracket 7, the paper sample rack is located on the side of the test tube away from the test tube holder, the paper sample rack is provided with an alignment port 8, the alignment port 8 is aligned with the opening of the test tube, the paper sample rack is used to place a paper sample carrying a sample, and the sample on the paper sample is located in the alignment port 8;

[0042] A sampling assembly is installed on the bracket 7, and the sampling assembly includes a housing, on which a laser transmitter 9, a first preservation liquid releaser 10, a controller, a first driver 4 and an air gun 11 are bolted. The first driver 4 is a cylinder, and a compression ring 12 is bolted on the output shaft of the first driver 4;

[0043] The laser transmitter 9 is used to emit laser light for burning the paper sample;

[0044] The first preservation solution releaser 10 is used to store and release the preservation solution onto the paper sample;

[0045] The first driver 4 is used to make the pressing ring 12 press the paper sample after the paper sample is placed;

[0046] The air gun 11 is used to make the paper sample concave toward the test tube by blowing out air after the paper sample is placed;

[0047] The controller is used to control the laser emitter 9 to burn a through hole on the paper sample outside the sample after placing the paper sample, and then control the first preservation liquid releaser 10 to release the preservation liquid, so that the preservation liquid flushes the sample and enters the test tube through the through hole and the alignment port 8.

[0048] When in use, the patient needs to prepare a paper sample to carry the sample. The paper sample is a tissue used by the patient to wipe feces and other excretions or body fluids. The tissue should be able to be burned with regular through holes by the laser emitter 9, and at the same time, after being soaked in the preservation liquid, it still maintains sufficient toughness to carry the preservation liquid in the depression.

[0049] After the patient is ready to carry the paper sample of the sample, he / she will place the test tube bound with his / her own information barcode on the automatic capping machine 6, lay the paper sample on the paper sample rack, and start the sampling component.

[0050] The sampling assembly controls the first driver 4 to drive the pressing ring 12 so that the pressing ring 12 presses the paper sample, and controls the air gun 11 to make the paper sample concave toward the alignment port 8 by blowing air. The laser emitter 9 is controlled to emit laser light to burn a through hole on the paper sample, and the air gun 11 can blow air again to discharge the debris generated by the burning through the through hole. At the same time, the automatic capping machine 6 screws up the cover of the test tube and moves the test tube to the bottom of the alignment port 8 through the second driving member. The time point when the test tube moves to the bottom of the alignment port 8 is after the debris is discharged from the through hole, so as to reduce the probability of the debris falling into the test tube during the burning.

[0051] After the test tube moves to the position directly below the alignment port 8, the first preservation liquid releaser 10 injects preservation liquid into the depression, and the preservation liquid will flush the sample to mix the sample with the preservation liquid. The diameter of the through hole burned out satisfies that the outflow rate of the preservation liquid through the through hole is less than the release rate of the first preservation liquid releaser 10. After the preservation liquid is released, it will not flow directly into the test tube through the through hole, but will be temporarily stored in the depression to fully contact with the sample. At the same time, the preservation liquid in the depression will generate turbulence as the first preservation liquid releaser 10 continues to release, thereby enhancing the mixing effect.

[0052] The sampling component also includes an image capturer 13, which is a camera. The camera bolts are fixed to the bracket 7. The image capturer 13 is used to obtain sample images. The controller is used to identify the sample amount through grayscale edge detection. The controller is preset with the corresponding test tube carrying capacity of each sample amount. When the preservation solution flowing into the test tube reaches the test tube carrying capacity, the second driver 5 is controlled to make the test tube coordination seat 3 carry the test tube to the automatic capping machine 6.

[0053] Although the sample can be directly sampled through the paper sample as a medium, the sample amount on the paper sample is not fixed. Some patients may have too much sample on the paper sample, which will cause the solid content of the sample in the preservation solution to be too high, which is not convenient for subsequent detection. The image capture device 13 can detect the sample image. Since the sample color is significantly different from the paper sample, the edge detection of the sample can be quickly performed through grayscale to determine the amount of the sample. When the sample amount is large, the amount of preservation solution accepted in the test tube is reduced to control the solid content in the preservation solution during subsequent detection.

[0054] A second preservation liquid releaser 14 is bolted to the automatic capping machine 6. Both the first preservation liquid releaser 10 and the second preservation liquid releaser 14 are electric syringes. The controller is used to inject preservation liquid into the test tube before the automatic capping machine 6 screws the cap on the test tube, so that the total amount of preservation liquid in the test tube reaches a preset value.

[0055] After the sample solid is restrained, the amount of preservation solution in the test tube is not equal, and the concentration difference is also large. The second preservation solution releaser 14 can replenish the preservation solution in the test tube, so that the amount of preservation solution and the sample concentration in each test tube are within a reasonable fluctuation range.

[0056] The sampling assembly also includes a projector 15, which is bolted to the casing. The projector 15 is used to project the effective range of the preservation solution on the paper sample after the paper sample is placed.

[0057] The projector 15 can project the effective range on the paper sample, which is convenient for standardizing the placement position of the sample on the paper sample.

[0058] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A sampling device for bacteria detection, characterized in that: The invention comprises a test tube holder, to which a test tube is detachably connected, a bracket (7) is fixedly connected to the test tube holder, a paper sample holder is fixedly connected to the bracket (7), the paper sample holder is located on a side of the test tube away from the test tube holder, an alignment opening (8) is provided on the paper sample holder, the alignment opening (8) is aligned with the opening of the test tube, the paper sample holder is used to place a paper sample carrying a sample, and the sample on the paper sample is located in the alignment opening (8); A sampling assembly is fixedly connected to the bracket (7), and the sampling assembly comprises a laser emitter (9), a first preservation liquid releaser (10) and a controller; The laser emitter (9) is used to emit laser light for burning the paper sample; The first preservation liquid releaser (10) is used to store and release the preservation liquid onto the paper sample; The controller is used to control the laser emitter (9) to burn a through hole on the paper sample outside the sample after placing the paper sample, and then control the first preservation liquid releaser (10) to release the preservation liquid, so that the preservation liquid flushes the sample and enters the test tube through the through hole and the alignment port (8).

2. The sampling device for bacteria detection according to claim 1, characterized in that: The sampling component also includes a pressing ring (12), and the pressing ring (12) is provided with a first driver (4). The first driver (4) is used to make the pressing ring (12) press the paper sample after the paper sample is placed.

3. The sampling device for bacteria detection according to claim 2, characterized in that: The sampling assembly also includes an air gun (11), which is used to make the paper sample concave towards the test tube by blowing out air after the paper sample is placed.

4. The sampling device for bacteria detection according to claim 3, characterized in that: The diameter of the burned through hole satisfies the requirement that the outflow rate of the preservation liquid from the through hole is less than the release rate of the first preservation liquid releaser (10).

5. The sampling device for bacteria detection according to claim 4, characterized in that: The test tube stand comprises a base (1), a guide rail (2) is provided on the base (1), a test tube coordination seat (3) is slidably connected to the guide rail (2), the test tube coordination seat (3) is detachably connected to the test tube, and the guide rail (2) is provided with a second driver (5) for driving the test tube coordination seat (3) to slide.

6. The sampling device for bacteria detection according to claim 5, characterized in that: A waste liquid storage tank is provided on the base (1), and the waste liquid storage tank is aligned with the alignment opening (8).

7. The sampling device for bacteria detection according to claim 6, characterized in that: An automatic capping machine (6) is provided on one side of the guide rail (2) away from the support (7). The automatic capping machine (6) is used to remove or screw on the cap of the test tube.

8. The sampling device for bacteria detection according to claim 7, characterized in that: The sampling component also includes a projector (15), which is used to project the effective range of the preservation liquid flushing on the paper sample after the paper sample is placed.

9. The sampling device for bacteria detection according to claim 8, characterized in that: The sampling component also includes an image capturer (13) for acquiring a sample image, and a controller for identifying the sample amount through grayscale edge detection. The controller is preset with a corresponding test tube carrying capacity for each sample amount. When the preservation liquid flowing into the test tube reaches the test tube carrying capacity, the second driver (5) is controlled to make the test tube coordination seat (3) carry the test tube and move toward the automatic capping machine (6).

10. The sampling device for bacteria detection according to claim 9, characterized in that: The automatic capping machine (6) is fixedly connected to a second preservation liquid releaser (14). The controller is used to inject preservation liquid into the test tube before the automatic capping machine (6) screws the cap on the test tube, so that the total amount of preservation liquid in the test tube reaches a preset value.

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