Reaction equipment for synthesizing mixed enzyme through fine glufosinate-ammonium biological method

Abstract

Relating to the field of mixed enzyme biological method reaction equipment, the invention discloses a refined glufosinate-ammonium biological method synthesis mixed enzyme reaction equipment, which comprises a reaction kettle and a rotating rod extending to the bottom of the reaction kettle, the reaction kettle is internally provided with a separation assembly, the bottom of the reaction kettle is provided with a gas transmission pipeline, and the tail end of the gas transmission pipeline is provided with an exhaust assembly. The neck-shaped part of the separation assembly limits the moving path of the material and separates the interior of the reaction kettle, so that the reaction kettle is divided into an upper half part and a lower half part, and the forward rotation and reverse rotation of the rotating rod are matched to change the circular flowing direction of the material and change the opening and closing state of the communicating vessel, thereby changing the exhaust position of the exhaust assembly and exhaust gas. The bubbles in the material can leave the material more quickly when the material entrained bubbles are located in the upper half part, and the bubbles in the material are difficult to separate when the material entrained bubbles are located in the lower half part, so that the contact time of the bubbles and the material is prolonged, and the quick and effective control required when the pH value of the material is changed is met.

Description

Technical Field

[0001] The present invention relates to the field of mixed enzyme biological reaction equipment, in particular to a mixed enzyme reaction equipment for the biological synthesis of refined glufosinate ammonium. Background Art

[0002] Glufosinate-ammonium is a widely used herbicide that achieves its weed-killing effect by inhibiting the amino acid synthesis of plants. In industrial production, the biosynthesis of glufosinate-ammonium is mainly achieved through microbial and mixed enzyme fermentation.

[0003] In the synthesis process, a bioreactor, i.e. a special reactor, is mainly used for fermentation. The reactions of microorganisms, mixed enzymes and other substances in the fermentation process usually have special requirements. For example, microorganisms and mixed enzymes usually need continuous oxygen to meet the aerobic conditions of microorganisms and mixed enzymes during the reaction process, and carbon dioxide is used to adjust the pH value that changes during the reaction process. Most of the existing gas injection methods directly set up different gas pipelines in the reactor and inject gas from bottom to top by setting exhaust nozzles on the surface of the gas pipelines. However, the gas injected into the reactor will quickly float to the surface of the material in the form of bubbles and then be discharged outward, resulting in limited contact time between the gas and the material. If the pH value changes rapidly and needs to be quickly adjusted by carbon dioxide, it cannot be guaranteed that the gas can effectively and quickly lower the pH value of the material in a short time. If the pH value cannot be effectively controlled in a short time, the entire material may be scrapped. Summary of the invention

[0004] In order to make up for the deficiencies of the prior art, the purpose of the present invention is to solve the problems existing in the prior art: the contact time between gas and materials in the existing reactor during the gas injection process is limited, and when special circumstances occur during the reaction process, the existing gas injection method is difficult to quickly adjust the pH.

[0005] In order to solve the problems of the prior art, the technical solution of the present invention is as follows: comprising a reactor and a rotating rod extending to the bottom of the reactor, and the rotating rod is connected to an external power device, an upwardly protruding partition component is arranged inside the reactor, a neck-shaped portion is arranged at the center of the partition component, a connecting component is arranged at the edge bottom of the partition component, and a connecting groove is opened on the surface of the connecting component; An impeller is arranged at the position where the rotating rod passes through the neck portion. When the impeller rotates forward, the material is transported downward along the neck portion of the partition component. When the impeller rotates reversely, the material is transported upward along the neck portion. A gas pipeline is arranged at the bottom of the reactor, and an exhaust component is arranged at the end of the gas pipeline.

[0006] Furthermore, there are two gas pipelines, the ends of the two gas pipelines are close to each other, and the two gas pipelines are a carbon dioxide pipeline and an oxygen pipeline respectively.

[0007] Furthermore, the exhaust assembly includes two connecting pipes respectively connected to two gas pipelines. A connecting vessel is provided on the inner side of the two connecting pipes and the two groups of exhaust pipes are respectively connected through the connecting vessel, so that the two exhaust pipes can be respectively connected to the two gas pipelines.

[0008] Furthermore, a limit bearing is provided at the bottom of the rotating rod, and a connecting column is provided at the bottom of the limit bearing. Two groups of inclined support rods are movably installed on one side of the surface of the connecting column close to the two connecting pipes. The ends of the support rods extend into the interior of the communicating vessel and are movably connected with plugs. The inclination directions of the two groups of support rods are opposite. The rotation angle of the connecting column is limited by the limit bearing, and the support rods with opposite inclination directions move in opposite ways when the connecting column rotates.

[0009] Furthermore, the communicating vessel is divided into a connecting end and a connecting cavity, the diameter of the plug is smaller than the inner diameter of the connecting end, and a rubber layer is provided on the side of the plug surface close to the support rod, and the diameter of the part of the plug provided with the rubber layer is larger than the inner diameter of the connecting end, and the connecting state between the connecting end and the connecting cavity is changed by moving the plug on both sides of the connecting cavity.

[0010] Furthermore, the connecting pipe is an annular pipeline, and the two connecting pipes are vertically arranged, and the connecting pipe connected to the oxygen pipeline is located at the bottom, and the connecting pipe connected to the carbon dioxide pipeline is located at the top, and the number of connecting vessels inside the connecting pipe connected to the oxygen pipeline is smaller than the connecting vessels inside the other connecting pipe, so that the amount of exhaust to the outside of the connecting pipe connected to the oxygen pipeline through the connecting vessels is smaller than that of the other connecting pipe.

[0011] Furthermore, the exhaust pipes are four concentrically arranged annular pipes, and the four exhaust pipes are divided into two groups, an inner group and an outer group. The two exhaust pipes in each group are connected. The two groups of exhaust pipes are respectively arranged at the top and bottom of the connecting pipe and are respectively connected to the two gas pipelines through the connecting vessel and the connecting pipe, wherein the exhaust pipe connected to the oxygen pipeline is the outer group, and the exhaust pipe connected to the carbon dioxide pipeline is the inner group, so as to distinguish the exhaust areas of the two groups of exhaust pipes.

[0012] Furthermore, the exhaust pipe connected to the oxygen pipeline in the gas transmission pipeline is arranged close to the connecting groove of the connecting component, and the exhaust pipe connected to the carbon dioxide pipeline is located inside the connecting component and is arranged higher than the connecting groove in the horizontal direction.

[0013] Furthermore, the partition component is composed of a top neck-shaped part and a bottom inclined part connected to the reactor. The connecting component is distributed in an annular manner at the bottom of the inclined part of the partition component. The connecting component is a rectangular box-shaped structure with an opening at the top, and the opening part passes through the partition component. The connecting groove is opened on the side of the connecting component close to the bottom to limit the movement path of the material in the reactor, and the neck-shaped part forms a tubular structure adapted to the impeller and forms an auger-shaped component with the impeller, and the partition component separates the reactor into two upper and lower parts.

[0014] Furthermore, a wavy circular plate is provided on the surface of the rotating rod, and the wavy circular plate is located at a position of the rotating rod close to the connecting component. A stabilizing bracket connected to the inner wall of the reactor is provided on the surface of the rotating rod between the wavy circular plate and the exhaust component. The stabilizing bracket is rotatably connected to the rotating rod through a rotating shaft. The wavy circular plate will not drive the material to rotate during rotation but will stir the material, and the wavy circular plate can block the flowing material and bubbles. The stabilizing bracket stabilizes the vibration that may be generated by the wavy circular plate during rotation of the rotating rod, thereby ensuring a stable connection with the limit bearing.

[0015] Compared with the prior art, the advantages of the present invention are as follows: 1. The present invention limits the moving path of the material and separates the interior of the reactor by the neck-shaped part of the partition component, so that the reactor is divided into an upper half and a lower half. When the bubbles entrained by the material are located in the upper half, the bubbles in the material can leave the material faster to meet the circulation flow of the gas in the material, while when the bubbles entrained by the material are located in the lower half, the bubbles in the material are difficult to escape, so that the bubbles in the material can be in contact with the material for a long time, thereby meeting the rapid and effective control required when the pH value of the material changes.

[0016] 2. The present invention drives the rotating rod to rotate forward or reversely through a motor, and changes the opening and closing and connection status of the communicating vessel and the flow direction of the material during the rotation. The opening and closing and connection status of the communicating vessel determine the exhaust position and exhaust gas of the exhaust component. The different flow directions determine the contact time between oxygen and carbon dioxide and the material. During forward rotation, the material entrains oxygen into the upper part and circulates. The oxygen in the material can leave the material in time after the reaction to ensure that new oxygen can enter the material, thereby optimizing the reaction effect between the material and oxygen. During reverse rotation, the material entrains carbon dioxide in the lower part and is restricted by the partition component. The material is still circulating, but it is difficult for the gas to follow the circulation through the partition component, which increases the carbon dioxide bubbles in the lower part. The material in the lower part can come into contact with a large amount of carbon dioxide, thereby quickly reducing the pH value in the material. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0018] Figure 2 It is a schematic cross-sectional view of the overall structure of the present invention.

[0019] Figure 3 It is a schematic diagram of the structure of the partition assembly of the present invention.

[0020] Figure 4 It is a schematic diagram of the rotating rod structure of the present invention.

[0021] Figure 5 It is a schematic diagram of the connection of the exhaust assembly of the present invention.

[0022] Figure 6 It is a schematic diagram of the connecting column structure of the present invention.

[0023] Figure 7 It is a schematic diagram of the connecting pipe structure of the present invention.

[0024] Figure 8 It is a cross-sectional schematic diagram of the communicating vessel of the present invention.

[0025] Figure numerals: 1. Reactor; 2. Rotating rod; 201. Wave circular plate; 202. Limit bearing; 203. Connecting column; 204. Support rod; 205. Plug; 206. Stabilizing bracket; 3. Partitioning assembly; 4. Neck-shaped portion; 5. Connecting assembly; 501. Connecting groove; 6. Impeller; 7. Gas pipeline; 8. Exhaust assembly; 801. Connecting pipe; 802. Connecting vessel; 803. Exhaust pipe. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0027] like Figure 1-8As shown, a mixed enzyme reaction device for the biological synthesis of refined glufosinate ammonium comprises a reactor 1 and a rotating rod 2 extending to the bottom of the reactor 1, and the rotating rod 2 is connected to an external power device, an upwardly protruding partition component 3 is arranged inside the reactor 1, a neck-shaped portion 4 is arranged at the center of the partition component 3, a connecting component 5 is arranged at the edge bottom of the partition component 3, a wavy circular plate 201 is arranged on the surface of the rotating rod 2, the wavy circular plate 201 is located at a position of the rotating rod 2 close to the connecting component 5, a stabilizing bracket 206 connected to the inner wall of the reactor 1 is arranged at a position on the surface of the rotating rod 2 between the wavy circular plate 201 and the exhaust component 8, the stabilizing bracket 206 is rotatably connected to the rotating rod 2 through a rotating shaft, the wavy circular plate 201 will not drive the material to rotate during the rotation process but will stir the material, and the wavy circular plate 201 can block the flowing material and bubbles, and the stabilizing bracket 206 stabilizes the vibration of the rotating rod 2 during the rotation process due to the wavy circular plate 201, thereby ensuring a stable connection with the limit bearing 202, and a connecting groove 501 is provided on the surface of the connecting component 5; The portion where the rotating rod 2 passes through the neck portion 4 is provided with an impeller 6. The partition component 3 is composed of a top neck portion 4 and an inclined portion connected to the reactor 1 at the bottom. The connecting component 5 is annularly distributed at the bottom of the inclined portion of the partition component 3. The connecting component 5 is a rectangular box-shaped structure with an opening at the top, and the opening portion passes through the partition component 3. The connecting groove 501 is provided on one side of the connecting component 5 close to the bottom to limit the movement path of the material in the reactor 1. The neck portion 4 forms a tubular structure adapted to the impeller 6 and forms an auger-shaped component with the impeller 6. The partition component 3 divides the reactor 1 into two upper and lower parts. When the impeller 6 rotates forward, the material is transported downward along the neck portion 4 of the partition component 3. When the impeller 6 rotates reversely, the material is transported upward along the neck portion 4. A gas pipeline 7 is arranged at the bottom of the reactor 1. There are two gas pipelines 7. The ends of the two gas pipelines 7 are close to each other. The two gas pipelines 7 are respectively a carbon dioxide pipeline and an oxygen pipeline. An exhaust assembly 8 is arranged at the end of the gas pipeline 7. The exhaust assembly 8 includes two connecting pipes 801 respectively connected to the two gas pipelines 7. The inner sides of the two connecting pipes 801 are both provided with connecting vessels 802, and two groups of exhaust pipes 803 are respectively connected through the connecting vessels 802, so that the two exhaust pipes 803 can be respectively connected to the two gas pipelines 7. The connecting pipe 801 is an annular pipeline. The two connecting pipes 801 are arranged vertically, and the connecting pipe 801 connected to the oxygen pipeline is located at the bottom, and the connecting pipe 801 connected to the carbon dioxide pipeline is located at the top. The number of connecting vessels 802 inside the connecting pipe 801 connected to the oxygen pipeline is less than that of the connecting vessel 802 inside the other connecting pipe 801, so that the exhaust volume of the connecting pipe 801 connected to the oxygen pipeline through the connecting vessel 802 is less than that of the other connecting pipe 801. The exhaust pipes 803 are four concentrically arranged annular pipes, and the four exhaust pipes 803 are divided into two groups, inner and outer. The two exhaust pipes 803 in each group are connected. The two groups of exhaust pipes 803 are respectively arranged at the top and bottom of the connecting pipe 801 and are respectively connected to the two gas pipelines 7 through the connecting vessel 802 and the connecting pipe 801, wherein the exhaust pipe 803 connected to the oxygen pipeline is the outer group, and the exhaust pipe 803 connected to the carbon dioxide pipeline is the inner group, and the exhaust areas of the two groups of exhaust pipes 803 are distinguished. The exhaust pipe 803 connected to the oxygen pipeline in the gas pipeline 7 is arranged close to the connecting groove 501 of the connecting component 5, and the exhaust pipe 803 connected to the carbon dioxide pipeline is located on the inner side of the connecting component 5 and is higher than the connecting groove 501 in the horizontal direction; A limit bearing 202 is provided at the bottom of the rotating rod 2, and a connecting column 203 is provided at the bottom of the limit bearing 202. Two groups of inclined support rods 204 are movably installed on the side of the surface of the connecting column 203 close to the two connecting pipes 801. The ends of the support rods 204 extend into the interior of the communicating vessel 802 and are movably connected with plugs 205. The inclination directions of the two groups of support rods 204 are opposite. The rotation angle of the connecting column 203 is limited by the limit bearing 202, and the support rods 204 with opposite inclination directions move in opposite ways when the connecting column 203 rotates. The communicating vessel 802 is divided into a connecting end and a communicating cavity. The diameter of the plug 205 is smaller than the inner diameter of the connecting end, and a rubber layer is provided on the side of the surface of the plug 205 close to the support rod 204. The diameter of the part of the plug 205 with the rubber layer is larger than the inner diameter of the connecting end. The connecting end and the communicating cavity are changed by moving the plug 205 on both sides of the communicating cavity.

[0028] Working principle description: The present invention provides power through an external power device at the top of the rotating rod 2 to drive the rotating rod 2 and the impeller 6 and the wave circular plate 201 on its surface to rotate. In the normal use state, that is, the state of inputting oxygen, the rotating rod 2 rotates forward. When rotating forward, the rotating rod 2 drives the connecting column 203 to follow the rotation through the limit bearing 202 and limits the rotation angle of the connecting column 203, ensuring that the rotating rod 2 will not drive the connecting column 203 to rotate infinitely during normal rotation. After the connecting column 203 rotates, it drives the support rod 204 to move the plug 205, wherein the plug 205 located in the connecting vessel 802 of the connecting pipe 801 connected to the oxygen gas transmission pipeline 7 is pulled outward, and the plug 205 located in the other connecting vessel 802 is pulled outward. 5 is pushed inward, so that the connection state of the two communicating vessels 802 is reversed. At this time, oxygen enters the exhaust pipe 803 through the communicating vessel 802, and is discharged upward through the exhaust pipe 803 to mix with the material in the form of bubbles and move upward. During the movement, it contacts the wavy circular plate 201, and the wavy circular plate 201 stirs the material during the rotation process. In this process, the impeller 6 drives the material to pass through the partition component 3 and enter the upper half of the reactor 1. At this time, part of the bubbles entrained in the material will leave the material, and the other part of the bubbles will follow the material into the connecting component 5 again and return to the lower half of the reactor 1 through the connecting groove 501 to circulate, and complete the reaction with oxygen during the circulation process; During the reaction with oxygen, the mixed enzymes and microorganisms inside the material gradually react to saturation and reduce the oxygen demand. At the same time, they will release associated substances that affect the pH value of the material. When the operator needs to inject carbon dioxide to change the pH value according to the data reflected by the monitoring equipment, the external power device drives the rotating rod 2, and the impeller 6 and the wavy circular plate 201 on its surface to reverse. During the reversal process, the impeller 6 drives the material located in the upper half of the reactor 1 to move downward through the partition component 3 into the lower half of the reactor 1. At the same time, the rotating rod 2 drives the connecting column 203 to reverse through the limit bearing 202. After the connecting column 203 rotates, it drives the support rod 204 to move the plug 205, wherein the plug 205 is located in the connecting vessel 802 of the connecting pipe 801 connected to the carbon dioxide gas transmission pipeline 7. The material moves downward and the bubbles move upward to ensure sufficient mixing. The rotating wavy circular plate 201 stirs the material to further mix the bubbles and the material. At this time, the bubbles in the material are difficult to pass through the partition component 3 that is passing the material downward and continue to accumulate inside the lower half of the reactor 1 until the material entrains the bubbles and enters the connecting groove 501 at the bottom of the connecting component 5 and moves to the upper half of the reactor 1 through the connecting component 5. Only then can the bubbles leave the material, so that the material in the lower half of the reactor 1 can contact with a large number of carbon dioxide bubbles to quickly reduce the pH value.

[0029] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mixed enzyme reaction device for the biological synthesis of refined glufosinate ammonium, comprising a reactor (1) and a rotating rod (2) extending to the bottom of the reactor (1), wherein the rotating rod (2) is connected to an external power device, and is characterized in that: The reactor (1) is provided with an upwardly protruding partition component (3), a neck-shaped portion (4) is provided at the center of the partition component (3), a connecting component (5) is provided at the bottom of the edge of the partition component (3), and a connecting groove (501) is provided on the surface of the connecting component (5); An impeller (6) is provided at the portion where the rotating rod (2) passes through the neck portion (4). When the impeller (6) rotates forward, the material is transported downward along the neck portion (4) of the partition assembly (3). When the impeller (6) rotates reversely, the material is transported upward along the neck portion (4). A gas pipeline (7) is provided at the bottom of the reaction kettle (1), and an exhaust assembly (8) is provided at the end of the gas pipeline (7).

2. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: There are two gas pipelines (7), the ends of the two gas pipelines (7) are close to each other, and the two gas pipelines (7) are respectively a carbon dioxide pipeline and an oxygen pipeline.

3. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 2, characterized in that: The exhaust assembly (8) comprises two connecting pipes (801) respectively connected to two gas delivery pipelines (7); connecting vessels (802) are provided on the inner sides of the two connecting pipes (801) and are respectively connected to two groups of exhaust pipes (803) via the connecting vessels (802).

4. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 3, characterized in that: A limit bearing (202) is provided at the bottom of the rotating rod (2), a connecting column (203) is provided at the bottom of the limit bearing (202), two groups of inclined support rods (204) are movably mounted on one side of the surface of the connecting column (203) close to the two connecting pipes (801), the ends of the support rods (204) extend into the interior of the communicating vessel (802) and are movably connected to a plug (205), and the two groups of support rods (204) are inclined in opposite directions.

5. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 4, characterized in that: The communicating vessel (802) is divided into a connecting end and a connecting cavity, the diameter of the plug (205) is smaller than the inner diameter of the connecting end, a rubber layer is provided on the surface of the plug (205) close to the support rod (204), and the diameter of the portion of the plug (205) provided with the rubber layer is larger than the inner diameter of the connecting end.

6. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 3, characterized in that: The connecting pipe (801) is an annular pipeline, and the two connecting pipes (801) are arranged vertically, and the connecting pipe (801) connected to the oxygen pipeline is located at the bottom and the connecting pipe (801) connected to the carbon dioxide pipeline is located at the top, and the number of connecting vessels (802) inside the connecting pipe (801) connected to the oxygen pipeline is less than the number of connecting vessels (802) inside the other connecting pipe (801).

7. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 6, characterized in that: The exhaust pipes (803) are four concentrically arranged annular pipes. The four exhaust pipes (803) are divided into two groups, an inner group and an outer group. The two exhaust pipes (803) in each group are connected to each other. The two groups of exhaust pipes (803) are respectively arranged at the top and bottom of the connecting pipe (801) and are respectively connected to the two gas pipelines (7) through the connecting vessel (802) and the connecting pipe (801), wherein the exhaust pipes (803) connected to the oxygen pipeline are the outer group, and the exhaust pipes (803) connected to the carbon dioxide pipeline are the inner group.

8. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 7, characterized in that: The exhaust pipe (803) connected to the oxygen pipeline in the gas transmission pipeline (7) is arranged close to the connecting groove (501) of the connecting component (5), and the exhaust pipe (803) connected to the carbon dioxide pipeline is located inside the connecting component (5) and is arranged higher than the connecting groove (501) in the horizontal direction.

9. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The partition component (3) is composed of a neck-shaped portion (4) at the top and an inclined portion at the bottom connected to the reaction kettle (1); the connecting component (5) is distributed in an annular manner at the bottom of the inclined portion of the partition component (3); the connecting component (5) is a rectangular box-shaped structure with an opening at the top, and the opening portion passes through the partition component (3); and the connecting groove (501) is provided on one side of the connecting component (5) close to the bottom.

10. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The surface of the rotating rod (2) is provided with a wavy circular plate (201), the wavy circular plate (201) being located at a position of the rotating rod (2) close to the connecting component (5), and a stabilizing bracket (206) connected to the inner wall of the reaction kettle (1) is provided at a position of the surface of the rotating rod (2) between the wavy circular plate (201) and the exhaust component (8), the stabilizing bracket (206) being rotatably connected to the rotating rod (2) via a rotating shaft.

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