Anti-pollution filling device and method for cell culture tank
By designing an anti-pollution filling device that includes a protective box, display monitoring screen, sealed door, anti-pollution filling assembly and disinfection gas atomization column, the problem of bacteria entering the tank during cell culture is solved, and a more efficient anti-pollution effect and a stable culture environment are achieved.
Patent Information
- Application Number
- CN202411986433.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-03-28
AI Technical Summary
During cell culture, every time the culture tube lid is opened for solvent refill, bacteria can easily enter the tank, affecting the culture effect.
An anti-pollution filling device including a protective box, a display monitoring screen, a sealed door, an anti-pollution filling assembly and a disinfection gas atomization column is designed. The sealing and disinfection of the cell culture tank is achieved through the sealing door and a disinfection gas atomization column, and the anti-pollution filling operation is performed using the anti-pollution filling assembly.
Effectively prevent bacteria from entering the cell culture tank, improve the overall anti-pollution effect, and ensure the stability and reliability of the culture environment.
Smart Images
Figure CN119709405A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cell culture, and in particular to an anti-pollution filling device and a filling method for a cell culture tank. Background Art
[0002] Cell culture tanks are commonly used equipment in tissue engineering and stem cell research. They provide a closed culture environment to support the normal growth and development of cells and tissues, and provide a stable and reliable culture environment.
[0003] The key to anti-pollution measures for cell culture tanks is prevention, which should be carried out throughout the entire cell culture process. During cell culture, the culture tube cover needs to be opened each time to add the corresponding solvent. Due to each opening and closing, it is easy for bacteria to enter the interior, causing changes in the internal culture and affecting the overall cultivation effect.
[0004] Therefore, when culturing cells, it is necessary to open the lid of the culture tube each time to add the corresponding solvent. Due to each opening and closing, it is easy for bacteria to enter the interior, causing changes in the internal culture and affecting the overall cultivation effect. A contamination-proof filling device and filling method for a cell culture tank can be designed to solve the above-mentioned problems. Summary of the invention
[0005] In order to overcome the problem that during cell culture, the culture tube cover needs to be opened each time to add the corresponding solvent, and each opening and closing makes it easy for bacteria to enter the interior, causing changes in the internal culture and affecting the overall cultivation effect.
[0006] The technical solution of the present invention is: an anti-pollution filling device for a cell culture tank, comprising a protective box, a display monitoring screen, a sealing door, a first anti-pollution filling component, a second anti-pollution filling component, a third anti-pollution filling component and a disinfection gas atomization column; a protective box used to store cell culture tanks for protection, a display monitoring screen for monitoring the internal cell culture tank status feedback screen is provided on the top of the protective box, a sealing door for placing multiple cell culture tanks at one time to form a sealed protection with a single cell culture tank is provided at the outer end of the protective box, a disinfection gas atomization column is provided in the center of the protective box for atomizing and disinfecting the interior to prevent contamination, and the first anti-pollution filling component, the second anti-pollution filling component and the third anti-pollution filling component are provided on both sides of the protective box from bottom to top in sequence for pulling out the corresponding cell culture tanks for anti-pollution filling operations.
[0007] Preferably, a sealing door is provided at the outer end of the protective box for snap-fitting, so that multiple or a single cell culture tank can be placed at one time to form a sealed protection; a disinfecting gas atomizing column is provided at the center of the protective box, so that the interior can be atomized and disinfected to prevent contamination; a first anti-pollution filling component, a second anti-pollution filling component and a third anti-pollution filling component are provided on both sides of the protective box in sequence from bottom to top, so that the corresponding cell culture tanks can be pulled out for anti-pollution filling operations, thereby forming a multi-level anti-pollution structure and improving the overall anti-pollution effect.
[0008] Preferably, a chassis is provided at the bottom of the protective box, a connection hole for accommodating a disinfection gas atomization column is provided in the center of the chassis, a plurality of groups of bases are evenly distributed at the bottom of the chassis, docking grooves for installing a first anti-pollution filling component, a second anti-pollution filling component and a third anti-pollution filling component are provided on both sides of the protective box from bottom to top, an anti-pollution storage space is provided inside the protective box, and three groups of partitions are provided inside the anti-pollution storage space from bottom to top.
[0009] Preferably, air holes are distributed on the partition, a center hole is opened in the center of the partition, a sleeve for installing the partition is provided on the inner wall of the anti-pollution storage space, a first sealing strip is provided at the inner edge of the protective box, three groups of buckles are linearly provided on one side of the first sealing strip, and a sleeve is provided inside the buckle.
[0010] Preferably, a sealed box is provided at the bottom of the display monitoring screen, and a temperature sensor, a gas concentration sensor and a first camera which are electrically connected to the protective box are provided at the bottom of the sealed box.
[0011] Preferably, a hinge module is provided between the sealed door and the protective box, a first thermometer is provided at the center of the outer end of the sealed door, a handle is provided at the outer end of the sealed door near the edge, three groups of clips are linearly provided on the side end of the sealed door, clip columns are provided inside the clips, a sealing plug plate is provided on one side of the sealed door corresponding to the protective box, a second sealing strip is provided on the periphery of the sealing plug plate, and a second camera is distributed on the sealing plug plate.
[0012] Preferably, the first anti-pollution filling component, the second anti-pollution filling component and the third anti-pollution filling component all include a filling box, a docking box, a support plate, air flow holes, rollers, handles, inner slide grooves, pull plates, pull-out sleeves, filling holes, docking sleeves and a second thermometer. The filling boxes and the support plates are each arranged in three groups, corresponding to the first anti-pollution filling component, the second anti-pollution filling component and the third anti-pollution filling component that are lengthened in sequence.
[0013] Preferably, the interior of the filling box is hollow, and a pullout tray is provided at the bottom of the filling box. Air flow holes are distributed on one end of the tray. A connecting shaft is linearly provided at the bottom of one end of the tray. A plurality of groups of rollers are linearly provided laterally on the connecting shaft. A handle is provided at the other end of the tray. A docking box is provided at the top of the filling box. An inner slide groove is provided on the inner side of the docking box near the top. A pull plate is provided inside the inner slide groove. A pull-out sleeve is provided at one end of the pull plate. A docking sleeve is provided on the docking box corresponding to the pull plate. A second thermometer is provided on the side end of the filling box, and five filling holes are linearly provided on the pull plate in ascending order.
[0014] Preferably, a connecting ring is linearly provided on the disinfection gas atomization column, a spray hole is circumferentially opened on the outer side of the connecting ring, an injector is provided inside the spray hole, a plug-in and disassembly box is provided on the top of the disinfection gas atomization column, a water pipe connected to the spray hole is provided at the bottom of the plug-in and disassembly box, a bacteria monitor is provided in the center of the plug-in and disassembly box, a cable is provided at the bottom of the bacteria monitor, and a PLC control cabinet is provided in the extending direction of the cable.
[0015] According to another aspect of the present invention, a method for filling a cell culture tank is provided, comprising the following steps: S1. According to the culture experiment to be carried out as needed, use the handle to open the sealed door, remove the plug-in and disassembly box, fill in the disinfection solvent, close the sealed door, use the PLC control cabinet to control the bacteria monitor through the cable to monitor the internal state, control each injector in turn to perform atomization spray disinfection, then open the sealed door, place the tanks that need to culture cells on the pallet in turn, install the retention device on the cell culture tank, and stagger the position of the air flow hole; S2. Close the sealing door, make sure the card strip and the buckle are aligned, insert the card column into the card sleeve to complete the installation, debug the display monitoring screen, use the temperature sensor, gas concentration sensor, the first camera and the second camera to monitor the whole system, and wait for the first thermometer and the second thermometer to reach the appropriate storage temperature; S3. When filling and culturing are required, first observe the display monitoring screen to find the cell culture tank of the corresponding level, select one of the first anti-pollution filling component, the second anti-pollution filling component and the third anti-pollution filling component, pull the handle to drive the support plate, and the support plate slides into the filling by rolling; S4. Fill the pipette gun to the required size. First, pull out the pull sleeve to drive the pull plate to slide along the inner slide groove to select the appropriate filling hole for operation. Then close the seal in turn, and the disinfection gas atomization column performs the atomization operation again.
[0016] Beneficial effects of the present invention: 1. During cell culture, it is necessary to open the culture tube cover each time to add the corresponding solvent. Each time it is opened and closed, it is easy for bacteria to enter the inside, causing changes in the internal culture and affecting the overall cultivation effect. Differently, a protective box can be used to store cell culture tanks for protection. A display monitoring screen is arranged on the top of the protective box, which can be used to monitor the internal cell culture tank status feedback screen. A sealing door is arranged at the outer end of the protective box, which can be used to place multiple or single cell culture tanks at one time to form a sealed protection. A disinfection gas atomization column is arranged in the center of the protective box, which can be used to perform atomization disinfection on the inside to prevent contamination. A first anti-pollution filling component, a second anti-pollution filling component and a third anti-pollution filling component are arranged on both sides of the protective box from bottom to top, which can be used to pull out the corresponding cell culture tanks for anti-pollution filling operations, forming a multi-level anti-pollution structure to improve the overall anti-pollution effect.
[0017] 2. When performing anti-pollution filling, observe the display monitoring screen to find the corresponding level of cell culture tanks, select one of the first anti-pollution filling component, the second anti-pollution filling component and the third anti-pollution filling component, pull the handle to drive the support plate, and the support plate slides into the filling by rolling. The size of the pipette gun needs to be filled. First, the pulling sleeve drives the pull plate to slide along the inner slide groove to select the appropriate filling hole for operation, and then the seals are closed in turn. The disinfection gas atomization column performs the atomization operation again to increase the overall anti-pollution operation performance. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 This is a schematic diagram of the overall structure of the anti-pollution filling device of the present invention; Figure 2 It is a schematic diagram of the protection box and the sealing door of the anti-pollution filling device of the present invention; Figure 3 This is a schematic diagram of a sealing door of the anti-pollution filling device of the present invention; Figure 4 This is a schematic diagram of a first anti-pollution filling component of the anti-pollution filling device of the present invention; Figure 5 This is a schematic diagram of a support plate of the anti-pollution filling device of the present invention; Figure 6 It is a schematic diagram of the sterilizing gas atomization column of the anti-pollution filling device of the present invention.
[0019] Description of reference numerals: 1. protection box; 2. display monitoring screen; 3. sealing door; 4. first anti-pollution filling assembly; 5. second anti-pollution filling assembly; 6. third anti-pollution filling assembly; 7. disinfection gas atomization column; 101. chassis; 102. base; 103. through-hole; 104. first sealing strip; 105. anti-pollution storage space; 106. docking groove; 107. buckle; 108. card sleeve; 109. sleeve; 110. partition; 111. air vent; 201. temperature sensor; 202. gas concentration sensor; 203. first camera; 301. hinge module; 302 , first thermometer; 303, handle; 304, card strip; 305, sealing plug plate; 306, second sealing strip; 307, second camera; 308, card column; 401, filling box; 402, docking box; 403, support plate; 404, air flow hole; 405, roller; 406, grip; 407, inner slide groove; 408, pull plate; 409, pull-out sleeve; 410, filling hole; 411, docking sleeve; 412, second thermometer; 701, PLC control cabinet; 702, cable; 703, connecting ring; 704, bacteria monitor; 705, injection hole; 706, plug-in and disassembly box. DETAILED DESCRIPTION
[0020] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0021] See also Figure 1-3 The present invention provides an embodiment: an anti-pollution filling device for a cell culture tank, comprising a protection box 1, a display monitoring screen 2, a sealing door 3, a first anti-pollution filling component 4, a second anti-pollution filling component 5, a third anti-pollution filling component 6 and a disinfection gas atomization column 7; a protection box 1 for storing cell culture tanks to form protection, a display monitoring screen 2 for monitoring the internal cell culture tank status feedback screen is provided on the top of the protection box 1, a sealing door 3 for placing multiple cells at one time to form a sealed protection with a single cell culture tank is provided at the outer end of the protection box 1, a disinfection gas atomization column 7 for atomizing and disinfecting the interior to prevent contamination is provided in the center of the protection box 1, and a first anti-pollution filling component 4, a second anti-pollution filling component 5 and a third anti-pollution filling component 6 for pulling out the corresponding cell culture tank for anti-pollution filling operation are provided on both sides of the protection box 1 from bottom to top.
[0022] See also Figure 1-4In this embodiment, a chassis 101 is provided at the bottom of the protective box 1, a through hole 103 for accommodating the disinfection gas atomization column 7 is provided in the center of the chassis 101, a plurality of bases 102 are evenly distributed at the bottom of the chassis 101, docking grooves 106 for installing the first anti-pollution filling component 4, the second anti-pollution filling component 5 and the third anti-pollution filling component 6 are provided on both sides of the protective box 1 from bottom to top, and an anti-pollution storage space 105 is provided inside the protective box 1, and three anti-pollution storage spaces 105 are provided inside the anti-pollution storage space 105 from bottom to top. A group of partitions 110 are used. Three groups of partitions 110 can be used for separated storage. There are air holes 111 distributed on the partitions 110. A center hole is opened in the center of the partition 110. The inner wall of the anti-pollution storage space 105 is provided with a sleeve 109 for installing the partition 110. A first sealing strip 104 is provided at the inner edge of the protective box 1. Three groups of buckles 107 are linearly provided on one side of the first sealing strip 104. A sleeve 108 is provided inside the buckle 107. The first sealing strip 104 can improve the protective sealing performance.
[0023] See also Figure 3-5 In this embodiment, a sealed box is provided at the bottom of the display monitoring screen 2, and a temperature sensor 201, a gas concentration sensor 202 and a first camera 203 which are electrically connected to the protective box 1 are provided at the bottom of the sealed box. A hinge module 301 is provided between the sealed door 3 and the protective box 1, a first thermometer 302 is provided at the center of the outer end of the sealed door 3, a handle 303 is provided at the outer end of the sealed door 3 near the edge, three groups of card strips 304 are linearly provided at the side end of the sealed door 3, and a card column 308 is provided inside the card strip 304. A sealing plug plate 305 is provided on one side of the sealed door 3 corresponding to the protective box 1, a second sealing strip 306 is provided on the periphery of the sealing plug plate 305, and a second camera 307 is distributed on the sealing plug plate 305. The temperature sensor 201 model is DHT11, the gas concentration sensor 202 model is ZE27-O3, and the first camera 203 and the second camera 307 model are ds-a71048r-icvs to monitor the internal feedback data to the display monitoring screen 2.
[0024] See also Figure 4-5In this embodiment, the first anti-pollution filling component 4, the second anti-pollution filling component 5 and the third anti-pollution filling component 6 all include a filling box 401, a docking box 402, a support plate 403, an air flow hole 404, a roller 405, a handle 406, an inner slide groove 407, a pull plate 408, a pull sleeve 409, a filling hole 410, a docking sleeve 411 and a second thermometer 412. The filling box 401 and the support plate 403 are all three sets of settings corresponding to the first anti-pollution filling component 4, the second anti-pollution filling component 5 and the third anti-pollution filling component 6 being lengthened in sequence. The interior of the filling box 401 is hollow, and a pullable support plate 403 is provided at the bottom of the filling box 401. There is air distribution on one end of the support plate 403. A flow hole 404 is provided, a connecting shaft is linearly provided at the bottom of one end of the support plate 403, a plurality of rollers 405 are linearly provided laterally on the connecting shaft, a handle 406 is provided at the other end of the support plate 403, a docking box 402 is provided on the top of the filling box 401, an inner slide groove 407 is provided on the inner side near the top of the docking box 402, a pull plate 408 is provided inside the inner slide groove 407, a pull sleeve 409 is provided at one end of the pull plate 408, a docking sleeve 411 is provided on the docking box 402 corresponding to the pull plate 408, a second thermometer 412 is provided on the side end of the filling box 401, five filling holes 410 are linearly provided on the pull plate 408 in ascending order, and the five filling holes 410 in ascending order can be used to adapt to pipettes of different calibers.
[0025] See also Figure 1-6 In this embodiment, a connecting ring 703 is linearly provided on the disinfection gas atomization column 7, a spray hole 705 is circumferentially opened on the outer side of the connecting ring 703, an injector is provided inside the spray hole 705, a plug-in and disassembly box 706 is provided on the top of the disinfection gas atomization column 7, a water pipe connected to the spray hole 705 is provided at the bottom of the plug-in and disassembly box 706, a bacteria monitor 704 is provided at the center of the plug-in and disassembly box 706, a cable 702 is provided at the bottom of the bacteria monitor 704, and a PLC control cabinet 701PLC is provided in the extending direction of the cable 702, and the internal bacteria situation is monitored through the bacteria monitor 704, model LB-6300.
[0026] According to another aspect of the present invention, a method for filling a cell culture tank is provided, comprising the following steps: S1. According to the culture experiment required, use the handle 303 to open the sealed door 3, remove the plug-in and disassembly box 706, inject the disinfectant, close the sealed door 3, use the PLC control cabinet 701PLC to control the bacteria monitor 704 through the cable 702 to monitor the internal state, control each injector in turn to perform atomization spray disinfection, then open the sealed door 3, place the tanks that need to culture cells on the support plate 403 in turn, install the retention device on the cell culture tank, and stagger the position of the air flow hole 404; S2, close the sealing door 3, note that the card strip 304 corresponds to the buckle 107, the card column 308 enters the card sleeve 108 to complete the installation, debug the display monitoring screen 2, use the temperature sensor 201, the gas concentration sensor 202, the first camera 203 and the second camera 307 to monitor the whole, and wait for the first thermometer 302 and the second thermometer 412 to reach the incubation temperature of 37°; S3, when filling and culturing are needed, first observe the display monitoring screen 2 to find the cell culture tank of the corresponding level, select one of the first anti-pollution filling component 4, the second anti-pollution filling component 5 and the third anti-pollution filling component 6, pull the handle 406 to drive the support plate 403, and the support plate 403 slides into the filling by rolling; S4. Fill the pipette gun to the required size. First, pull out the sleeve 409 to drive the pull plate 408 to slide along the inner slide groove 407 to select a suitable filling hole 410 for operation, and then close the seals in turn, and the disinfection gas atomization column 7 performs atomization operation again.
[0027] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the spirit of the present invention.
Claims
1. A contamination-proof filling device for a cell culture tank, comprising a protective box (1); characterized in that: The invention also comprises a display monitoring screen (2), a sealing door (3), a first anti-pollution filling component (4), a second anti-pollution filling component (5), a third anti-pollution filling component (6) and a disinfection gas atomizing column (7); a protective box (1) for storing cell culture tanks for protection; a display monitoring screen (2) for monitoring the feedback screen of the status of the internal cell culture tanks is provided on the top of the protective box (1); a sealing door (3) for placing a plurality of cell culture tanks at one time to form a sealed protection with a single cell culture tank is provided at the outer end of the protective box (1); a disinfection gas atomizing column (7) for atomizing and disinfecting the interior to prevent contamination is provided at the center of the protective box (1); and a first anti-pollution filling component (4), a second anti-pollution filling component (5) and a third anti-pollution filling component (6) for pulling out the corresponding cell culture tanks for anti-pollution filling operations are provided on both sides of the protective box (1) from bottom to top.
2. The anti-pollution filling device for a cell culture tank according to claim 1, characterized in that: A bottom plate (101) is provided at the bottom of the protection box (1), a through hole (103) for accommodating a disinfecting gas atomizing column (7) is provided at the center of the bottom plate (101), a plurality of bases (102) are evenly distributed at the bottom of the bottom plate (101), docking grooves (106) for installing a first anti-pollution filling component (4), a second anti-pollution filling component (5) and a third anti-pollution filling component (6) are provided on both sides of the protection box (1) from bottom to top, and an anti-pollution storage space (105) is provided inside the protection box (1), and three groups of partitions (110) are provided inside the anti-pollution storage space (105) from bottom to top.
3. The anti-pollution filling device for a cell culture tank according to claim 2, characterized in that: The partition (110) is provided with air holes (111) at the beginning, the center of the partition (110) is provided with a center hole, the inner wall of the anti-pollution storage space (105) is provided with a sleeve (109) for installing the partition (110), the inner edge of the protective box (1) is provided with a first sealing strip (104), one side of the first sealing strip (104) is linearly provided with three groups of buckles (107), and the inside of the buckle (107) is provided with a clamping sleeve (108).
4. The anti-pollution filling device for a cell culture tank according to claim 1, characterized in that: A sealed box is provided at the bottom of the display monitoring screen (2), and a temperature sensor (201), a gas concentration sensor (202) and a first camera (203) are provided at the bottom of the sealed box, which are electrically connected to the protective box (1).
5. The anti-pollution filling device for a cell culture tank according to claim 1, characterized in that: A hinge module (301) is provided between the sealed door (3) and the protective box (1); a first thermometer (302) is provided at the center of the outer end of the sealed door (3); a handle (303) is provided at the outer end of the sealed door (3) near the edge; three groups of clamping strips (304) are linearly provided at the side end of the sealed door (3); clamping columns (308) are provided inside the clamping strips (304); a sealing plug plate (305) is provided on one side of the sealed door (3) corresponding to the protective box (1); a second sealing strip (306) is provided on the outer periphery of the sealing plug plate (305); and a second camera (307) is distributed on the sealing plug plate (305).
6. The anti-pollution filling device for a cell culture tank according to claim 1, characterized in that: The first anti-pollution filling assembly (4), the second anti-pollution filling assembly (5) and the third anti-pollution filling assembly (6) all include a filling box (401), a docking box (402), a support plate (403), an air flow hole (404), a roller (405), a handle (406), an inner slide groove (407), a pull plate (408), a pull sleeve (409), a filling hole (410), a docking sleeve (411) and a second thermometer (412). The filling box (401) and the support plate (403) are all arranged in three groups, corresponding to the first anti-pollution filling assembly (4), the second anti-pollution filling assembly (5) and the third anti-pollution filling assembly (6) being lengthened in sequence.
7. The anti-pollution filling device for a cell culture tank according to claim 6, characterized in that: The filling box (401) is hollow inside. A pull-out support plate (403) is provided at the bottom of the filling box (401). Air flow holes (404) are distributed on one end of the support plate (403). A connecting shaft is linearly provided at the bottom of one end of the support plate (403). A plurality of rollers (405) are linearly provided on the connecting shaft. A handle (406) is provided at the other end of the support plate (403). A docking box (402) is provided at the top of the filling box (401). An inner slide groove (407) is provided on the inner side of the docking box (402) near the top. A pull plate (408) is provided inside the inner slide groove (407). A pull-out sleeve (409) is provided at one end of the pull plate (408). A docking sleeve (411) is provided on the docking box (402) corresponding to the pull plate (408). A second thermometer (412) is provided at the side end of the filling box (401). Five filling holes (410) are linearly provided on the pull plate (408) in ascending order.
8. The anti-pollution filling device for a cell culture tank according to claim 1, characterized in that: A connecting ring (703) is linearly provided on the disinfecting gas atomizing column (7), a spray hole (705) is circumferentially opened on the outer side of the connecting ring (703), an injector is provided inside the spray hole (705), a plug-in and disassembly box (706) is provided on the top of the disinfecting gas atomizing column (7), a water pipe connected to the spray hole (705) is provided at the bottom of the plug-in and disassembly box (706), a bacteria monitor (704) is provided at the center of the plug-in and disassembly box (706), a cable (702) is provided at the bottom of the bacteria monitor (704), and a PLC control cabinet (701) is provided in the extension direction of the cable (702).
9. A method for filling a cell culture tank, comprising the anti-pollution filling device for a cell culture tank according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. According to the culture experiment to be performed as required, the sealed door (3) is opened using the handle (303), the plug-in and disassembly box (706) is removed, a disinfectant solution is injected, the sealed door (3) is closed, the bacteria monitor (704) is controlled by the PLC control cabinet (701) through the cable (702) to monitor the internal state, and each injector is controlled in turn to perform atomization spray disinfection, and then the sealed door (3) is opened, the tanks to be cultured cells are placed in turn on the support plate (403), and the retention device on the cell culture tank is installed, and the position of the air flow hole (404) is staggered to leave room; S2, close the sealing door (3), make sure that the card strip (304) corresponds to the buckle (107), the card column (308) enters the card sleeve (108) to complete the installation, debug the display monitoring screen (2), use the temperature sensor (201), the gas concentration sensor (202), the first camera (203) and the second camera (307) to monitor the whole, and wait for the first thermometer (302) and the second thermometer (412) to reach the appropriate storage temperature; S3, when filling and culturing are required, first observe the display monitoring screen (2) to find the cell culture tank of the corresponding level, select one of the first anti-pollution filling component (4), the second anti-pollution filling component (5) and the third anti-pollution filling component (6), pull the handle (406) to drive the support plate (403), and the support plate (403) is rolled and slid into the filling; S4. Fill the pipette gun to the required size. First, pull the sleeve (409) to drive the pull plate (408) to slide along the inner slide groove (407) to select a suitable filling hole (410) for operation, and then close the seals in turn, and the disinfection gas atomization column (7) performs the atomization operation again.