Kit for rapidly purifying viral nucleic acid from different types of liquid biological samples and extraction method
By combining lysate with protease K in the magnetic bead method of viral nucleic acid extraction and optimizing the composition ratio, the problem of low sample processing efficiency in the prior art is solved, and fast and efficient viral nucleic acid extraction is achieved, which is suitable for different types of liquid biological samples.
Patent Information
- Application Number
- CN202411961724.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-03-28
AI Technical Summary
When the existing magnetic bead method of viral nucleic acid extraction methods are used to treat samples containing complex matrix or low concentration of viral nucleic acid, the nucleic acid yield is low and the pretreatment time is long, which significantly reduces the extraction efficiency.
A kit is provided, including lysis binding solution, protease K solution, magnetic beads, washing solution I, washing solution II and eluent. By combining lysate with protease K and optimizing the composition ratio in the lysate, protease K digestion, cleavage and nucleic acid binding, three steps of simultaneous protease K digestion, cleavage and nucleic acid binding are carried out, simplifying the operation steps.
It is possible to quickly and efficiently purify viral nucleic acids from different types of liquid biological samples. The obtained viral nucleic acids can be directly used in downstream experiments, such as PCR, RT-PCR, and qPCR, which significantly improves the extraction efficiency and nucleic acid yield.