Recombinant porcine alpha and gamma interferon
Through the insect-baculovirus expression system and synonymous preference modification codons, combined with the use of phomin signal peptides, the defects of the pig α and γ interferon expression systems in the prior art were solved, efficient and stable co-expression and purification were achieved, and biological activity and production efficiency were improved.
Patent Information
- Application Number
- CN202510092737.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2025-03-28
AI Technical Summary
The existing expression systems have problems such as protein modification, inclusion body existence, and hybrid protein production when expressing pig α and γ interferon, which leads to increased purification difficulty and cost and affects activity.
The insect-baculovirus expression system was adopted to modify the codons through synonymous preferences, add phomin signal peptides to achieve extracellular expression, and recombinant vector construction and viral infection were carried out using pFastBacTMDual vector and DH10Bac cells to achieve efficient co-expression of pig α and gamma interferon.
The efficient expression of pig α and γ interferon is achieved, the protein structure is close to the natural state, the biological activity is high, the purification is easy to carry out, which reduces production costs and improves the quality and stability of the recombinant protein.