Application of efcaab2 gene in construction of hydrocephalus animal model and construction method of model
The hydrocephalus zebrafish model with efcab2 gene defect was constructed through CRISPR/Cas9 technology, which solved the problem of difficulty in studying the role of the efcab2 gene in hydrocephalus pathogenesis in the existing technology, and achieved in-depth research on the pathogenesis of hydrocephalus and the identification of potential therapeutic targets.
CN119709879AActive Publication Date: 2025-03-28TIANJIN UNIV
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Patent Information
- Application Number
- CN202411948953.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2044-12-27
AI Technical Summary
Technical Problem
The prior art is difficult to effectively study the role of the efcab2 gene in hydrocephalus pathogenesis, and there is a lack of effective animal models to study such diseases.
Method used
Through CRISPR/Cas9 gene knockout technology, an animal model of hydrocephalus with efcab2 gene defect was constructed to study the pathogenesis and potential therapeutic targets of hydrocephalus.
Benefits of technology
This method not only deepens the understanding of gene regulation during the onset of hydrocephalus with efcab2 gene defect, but also provides a potential therapeutic target for hydrocephalus, providing a high-level animal model for translational medicine and new drug research and development.
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Abstract
The invention discloses an application of an efcaab2 gene in construction of a hydrocephalus animal model and a construction method of the model, belongs to the technical field of animal model preparation in biology and basic medical research, and provides the application of the efcaab2 gene in construction of the hydrocephalus animal model, and the nucleotide sequence of the efcaab2 gene is as shown in SEQ ID NO.1. The invention also provides a method for constructing the hydrocephalus zebrafish animal model, which comprises the following steps: synthesizing gRNA and Cas9mRNA on the basis of an efcaab2 gene, and mixing to obtain a mixed solution; co-injecting the zebra fish embryos and a phenol red reagent to a zebra fish embryo I cell stage, and culturing to the 3th day after fertilization to obtain zebra fish embryos; and measuring the mRNA expression quantity of the efcaab2 gene, and observing the fourth ventricle, the spine and the pericardium. A CRISPR (clustered regularly interspaced short palindromic repeats) gene knockout technology is utilized to establish an efcaab2 gene defect hydrocephalus animal model, and an efcaab2 gene defect hydrocephalus pathogenesis and a potential treatment target are clarified.
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