Sampling device and sample application equipment

Abstract

The embodiment of the invention provides a sampling device and sample application equipment. The sampling device comprises a mounting rack; a main body of the driving assembly is arranged on the mounting frame; the sampling assembly comprises an operation needle connected to the driving end of the driving assembly and a cap pressing piece with a penetrating hole, the operation needle is at least used for sampling and can ascend and descend along the penetrating hole, and the driving assembly is arranged in the mode that after the cap pressing piece is driven to press a bottle cap of a sample bottle, the cap pressing piece is driven to rotate; the operation needle is driven to penetrate through the penetrating-out hole to extend into the sample bottle and suck sample liquid in the sample bottle, and after the operation needle completing liquid suction is driven to penetrate through the penetrating-out hole to be separated from the sample bottle, the cover pressing piece is driven to be separated from the bottle cover. Therefore, the operation needle cannot move along with the sample bottle, and dangerous accidents are avoided.

Description

Technical Field

[0001] The present invention relates to the field of sample spotting technology, in particular to a sampling device and a sample spotting equipment having the same. Background Art

[0002] The TLC spotting experiment has a wide range of applications in the fields of chemistry, biology, medicine, and environment. Taking the field of chemistry as an example, the TLC spotting experiment can be used to separate and identify compounds: by smearing the mixture to be separated on the TLC plate and then conducting an experimental analysis, the relative mobility and relative content of each component can be obtained, thereby achieving the separation and identification of the mixture; in addition, the TLC spotting experiment can also be used to analyze the progress and products of the reaction. By taking samples during the reaction and then analyzing them on the TLC plate, the progress of the reaction and the generation of the products can be understood.

[0003] Thin layer chromatography spotting experiments require liquid aspiration through a sampling device. Specifically, the sample liquid can be placed in a sample bottle. The cylinder of the sampling device can drive the operating needle to extend into the sample bottle, so that the sample liquid in the sample bottle can be aspirated. When the aspiration is completed, the cylinder can drive the operating needle to be withdrawn from the sample bottle, so that it can be separated from the sample bottle. In this way, the operating needle can perform subsequent spotting operations.

[0004] However, when the cylinder drives the operating needle to disengage from the sample bottle, the operating needle will rub against the bottle cap of the sample bottle, and the sample bottle will move together. In this way, under the influence of the sample bottle, the operating needle cannot perform subsequent sample spotting operations, so the reliability of the sample spotting device is poor. In addition, the sample bottle may be damaged by hitting other parts, which may lead to dangerous accidents. Summary of the invention

[0005] In order to at least partially solve the problems existing in the prior art, according to one aspect of the present invention, a sampling device is provided. The sampling device comprises: a mounting frame; a driving assembly, the main body of the driving assembly is arranged on the mounting frame; and a sampling assembly, the sampling assembly comprises an operating needle connected to the driving end of the driving assembly and a capping member having a through hole, the operating needle is at least used for sampling and can be raised and lowered along the through hole, and the driving assembly is configured to: after the capping member is driven to press the bottle cap of the sample bottle, the operating needle is driven to pass through the through hole and extend into the sample bottle and absorb the sample liquid in the sample bottle, and after the operating needle that has completed the liquid absorption is driven to pass through the through hole and detach from the sample bottle, the capping member is driven to detach from the bottle cap.

[0006] Exemplarily, the driving assembly includes a first driving member, a second driving member and a third driving member, the driving end of the first driving member is connected to the pressing member to drive the pressing member to press and detach the bottle cap, the driving end of the second driving member is connected to the main body of the operating needle to drive the operating needle to pass through the outlet hole and extend into and detach from the sample bottle, and the driving end of the third driving member is connected to the piston rod of the operating needle to drive the operating needle to aspirate liquid.

[0007] Exemplarily, the main body of the first driving member is arranged on the mounting frame, and the driving end of the first driving member is connected to the main body of the second driving member, the main body of the third driving member and the pressure cover member, so as to drive the second driving member, the third driving member, the operating needle and the pressure cover member to rise and fall synchronously, and the driving end of the second driving member is connected to the main body of the third driving member and the main body of the operating needle, so as to drive the third driving member and the operating needle to rise and fall synchronously.

[0008] Exemplarily, the driving assembly also includes a first connecting frame, a second connecting frame, a third connecting frame, a first slide rail and a second slide rail, the first connecting frame is connected between the mounting frame and the main body of the first driving member, the first slide rail is arranged on the first connecting frame, the second connecting frame is connected between the driving end of the first driving member and the main body of the second driving member, and is slidably connected to the first slide rail, the pressure cover member and the second slide rail are arranged on the second connecting frame, the third connecting frame is connected between the driving end of the second driving member and the main body of the third driving member, and is slidably connected to the second slide rail, and the main body of the operating needle is arranged on the third connecting frame.

[0009] Exemplarily, the capping member includes a capping plate and an expansion boss protruding downward from the capping plate, the through hole passes through the capping plate and the expansion boss, the driving assembly is used to drive the capping plate to press and disengage the bottle cap, and the expansion boss is used to expand the sealing layer on the bottle cap to separate the sealing layer from the operating needle.

[0010] Exemplarily, the expansion boss is in the shape of a cone that tapers downward.

[0011] Exemplarily, a positioning boss protrudes downward from the outer edge of the pressing plate, and the positioning boss is sleeved on the top of the bottle cap before the pressing plate presses the bottle cap.

[0012] Exemplarily, the sampling device also includes an elastic component, which includes a pressure rod and an elastic member. The upper end of the elastic member is connected to the driving end of the driving component and the lower end is connected to the pressure rod. The driving component is used to drive the pressure rod to press and disengage the bottle cap. The pressure rod presses the bottle cap when the expansion boss expands the sealing layer to compress the elastic member and make the elastic member have elastic potential energy to drive the pressure rod to protrude downward from the expansion boss.

[0013] Exemplarily, the pressing rod includes a pressing portion and an extension rod extending upward from the pressing portion, and the elastic member is sleeved on the extension rod and the lower end of the elastic member is connected to the pressing portion.

[0014] Exemplarily, the operating needle includes a needle tube, a needle and a piston rod, the driving end of the driving assembly is connected to the piston rod to drive the piston rod to rise and fall in the needle tube, and the needle is integrally connected to the lower end of the needle tube.

[0015] Exemplarily, the outer diameter of the needle is between 0.4 mm and 0.6 mm.

[0016] Exemplarily, the inner diameter of the needle is between 0.1 mm and 0.3 mm.

[0017] According to another aspect of the present invention, a spotting device is provided, which comprises any of the above sampling devices, a spotting table for placing a spotting plate, and a drive assembly for driving the operating needle to spot the absorbed sample liquid onto the spotting plate.

[0018] Exemplarily, the spotting device further includes a positioning component. A groove for placing the spotting plate is provided on the spotting table, and the positioning component is used to position the spotting plate in the groove.

[0019] Exemplarily, the spotting device also includes a detector and a controller electrically connected to the detector, wherein the detector is located on the side of the spotting plate and is used to detect the height of the non-transparent top surface of the spotting plate at an angle downward to generate and send a signal to the controller, and the controller is used to drive the operating needle to spot based on the signal so that the operating needle has an expected height difference with the non-transparent top surface when the driving component drives the operating needle to spot.

[0020] Exemplarily, the spotting device further includes a reference calibration block, and the driving assembly is further used to drive the lower end of the operating needle to contact the reference calibration block, so that the position of the operating needle is used as the reference position.

[0021] In the sampling device provided by the embodiment of the present invention, during the process in which the driving component drives the operating needle to disengage from the sample bottle, since the driving component drives the capping member to press the bottle cap of the sample bottle, even if the operating needle rubs against the bottle cap of the sample bottle during the disengagement process, the capping member can keep the sample bottle still. In this way, the operating needle will not move with the sample bottle. Therefore, the operating needle can smoothly perform subsequent spotting operations, the reliability of the sampling device is high, and the user experience is good. In addition, the fixed sample bottle will not be damaged, and the sampling device has a high safety. In addition, during the process in which the operating needle extends into the sample bottle and absorbs the sample liquid in the sample bottle, the sample bottle can also remain fixed under the pressure of the capping member, so as not to affect the operation of the operating needle, thereby avoiding the occurrence of dangerous accidents.

[0022] A series of simplified concepts are introduced in the summary of the invention, which will be further described in detail in the detailed description. The summary of the invention does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0023] The advantages and features of the present invention are described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The following drawings of the present invention are used as part of the present invention for understanding the present invention. The drawings show the embodiments of the present invention and their descriptions, and are used to explain the principles of the present invention. In the drawings,

[0025] Figure 1 is a perspective view of a sampling device according to an exemplary embodiment of the present invention;

[0026] Figure 2 for Figure 1 A perspective view of a partial structure of the sampling device shown in FIG.

[0027] Figure 3 for Figure 2 A front view of a partial structure of the sampling device shown in FIG.

[0028] Figure 4 for Figure 3 A cross-sectional view of a portion of the structure of the sampling device shown in FIG.

[0029] Figure 5 for Figure 2 A three-dimensional view of the gland member shown in FIG.

[0030] The above drawings include the following reference numerals:

[0031] 100, mounting frame; 110, first horizontal driving member; 120, second horizontal driving member; 200, driving assembly; 210, first driving member; 220, second driving member; 230, third driving member; 240, first connecting frame; 250, second connecting frame; 260, third connecting frame; 270, first slide rail; 280, second slide rail; 291, first lead screw; 292, first nut; 293, second lead screw; 294, first Two nuts; 300, sampling assembly; 310, operating needle; 311, needle tube; 312, needle; 313, piston rod; 320, cover member; 321, through hole; 322, expansion boss; 323, positioning boss; 324, cover plate; 325, pressure rod through hole; 331, needle base; 332, operating needle cover plate; 400, elastic assembly; 410, pressure rod; 411, pressing part; 412, extension rod; 420, elastic member. DETAILED DESCRIPTION

[0032] In the following description, a large amount of details are provided so that the present invention can be thoroughly understood. However, it will be appreciated by those skilled in the art that the following description only exemplarily illustrates a preferred embodiment of the present invention, and the present invention can be implemented without one or more such details. In addition, in order to avoid confusion with the present invention, some technical features well known in the art are not described in detail.

[0033] According to one aspect of the present invention, a sampling device is provided. The sampling device can be used to absorb the sample liquid in the sample bottle, and then subsequent operations such as spotting can be performed. The sampling device can be applied to any suitable device, including but not limited to a spotting device. Therefore, according to another aspect of the present invention, a spotting device is also provided. The sampling device and the spotting device of the embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0034] like Figure 1-5 As shown, the sampling device may include a mounting frame 100 , a drive assembly 200 , and a sampling assembly 300 .

[0035] The mounting frame 100 can be fixed to the ground or a tabletop, etc., so as to serve as a base of the sampling device. The structure of the mounting frame 100 can be arbitrary.

[0036] The main body of the driving assembly 200 may be disposed on the mounting frame 100. The driving assembly 200 includes, but is not limited to, a motor driving assembly, a cylinder driving assembly, and / or an electric cylinder driving assembly.

[0037] The sampling assembly 300 may include an operating needle 310 and a capping member 320. The operating needle 310 and the capping member 320 may be connected to the driving end of the driving assembly 200, respectively. The operating needle 310 may adopt various types of operating needles known in the art or that may appear in the future, for example, it may be a high-precision micro-operating needle. The capping member 320 may have a through hole 321. Under the drive of the driving assembly 200, the operating needle 310 may rise and fall along the through hole 321. The operating needle 310 is at least used for sampling, that is, the operating needle 310 has other functions in addition to the sampling function, for example, when the sampling assembly 300 is applied to a spotting device, the operating needle 310 also has the function of spotting. The driving assembly 200 may be configured such that after the capping member 320 is driven to press the bottle cap of the sample bottle, the operating needle 310 is driven to pass through the through hole 321 and extend into the sample bottle, and absorb the sample liquid in the sample bottle. After the operating needle 310 completes the aspiration, the driving assembly 200 can drive the operating needle 310 to pass through the through hole 321 and detach from the sample bottle, and then drive the capping member 320 to detach from the bottle cap of the sample bottle. The operating needle 310 can always be inserted in the through hole 321, and can also detach from the through hole 321 during the lifting process.

[0038] In practical application, the sampling device can be located above the sample bottle. Then, the driving assembly 200 can drive the capping member 320 to descend to press the bottle cap of the sample bottle, so that the sample bottle can be fixed. Then, the driving assembly 200 can drive the operating needle 310 to descend so that the operating needle 310 can pass through the hole 321, so that it can extend into the sample bottle, and then the sample liquid in the sample bottle can be absorbed. The capping member 320 and the operating needle 310 can be descended at the same time, or they can be descended in sequence, as long as the capping member 320 can press the bottle cap of the sample bottle before the operating needle 310 extends into the sample bottle to absorb liquid. When the operating needle 310 absorbs liquid, the driving assembly 200 can drive the operating needle 310 to rise, so that it can pass through the hole 321 and detach from the sample bottle. Then, the driving assembly 200 can drive the capping member 320 to rise, so that it can detach from the bottle cap of the sample bottle, and then the fixation of the sample bottle can be cancelled. When spotting, the sampling assembly 300 can be located above the spotting plate. Then, the driving assembly 200 can drive the operating needle 310 to rise, fall or remain stationary, so that the operating needle 310 has a desired height difference with the spotting plate. Then, the sampling assembly 300 can drive the operating needle 310 to spot the sample liquid it absorbs onto the spotting plate.

[0039] In summary, in the sampling device provided by the embodiment of the present invention, in the process of the driving component 200 driving the operating needle 310 to disengage from the sample bottle, since the driving component 200 drives the capping member 320 to press the bottle cap of the sample bottle, even if the operating needle 310 rubs against the bottle cap of the sample bottle during the disengagement process, the capping member 320 can also keep the sample bottle still. In this way, the operating needle 310 will not move with the sample bottle. Therefore, the operating needle 310 can smoothly perform subsequent spotting operations, the reliability of the spotting device is high, and the user experience is good. In addition, the fixed sample bottle will not be damaged, and the safety of the sampling device is high. And, in the process of the operating needle 310 extending into the sample bottle and absorbing the sample liquid in the sample bottle, under the pressure of the capping member 320, the sample bottle can also remain fixed, so as not to affect the operation of the operating needle 310 to perform the operation, thereby avoiding the occurrence of dangerous accidents.

[0040] For example, Figure 2-4As shown, the operating needle 310 may include a needle tube 311, a needle 312 and a piston rod 313. The needle 312 may be connected to the lower end of the needle tube 311. The piston rod 313 may be movably inserted into the upper end of the needle tube 311. The driving end of the drive assembly 200 may be connected to the piston rod 313, so that the piston rod 313 may be driven to rise and fall in the needle tube 311, so that the operating needle 310 may aspirate and spot samples. Specifically, under the drive of the drive assembly 200, the needle 312 may descend along the through hole 321, so that it may extend into the sample bottle. Then, the drive assembly 200 may drive the piston rod 313 to rise, so that the sample liquid in the sample bottle may be aspirated through the needle 312. After the operating needle 310 completes the aspiration, the drive assembly 200 may drive the needle 312 to rise along the through hole 321, so that it may pass through the through hole 321 and be separated from the sample bottle. When spotting, the drive assembly 200 can drive the piston rod 313 to descend, so that the sample liquid can be spotted on the spotting plate through the needle 312. The needle 312 can be integrally connected to the lower end of the needle tube 311 by any suitable method such as welding, bonding or injection molding. With this arrangement, the connection strength between the needle 312 and the needle tube 311 is relatively high, especially for the needle 312 of the micro-operation needle, which has a smaller diameter, so it is particularly important to increase the connection strength with the needle tube 311. In addition, compared with the detachable connection method between the needle tube and the needle, this solution can avoid the deviation of the position of the needle 312 due to installation errors, thereby affecting the aspiration and spotting.

[0041] Exemplarily, the outer diameter of the needle 312 may be between 0.4 mm and 0.6 mm. The outer diameter of the needle 312 may be, for example, 0.4 mm, 0.5 mm, or 0.6 mm. If the outer diameter of the needle 312 is too large, it will occupy a large space and its applicability will be poor. If the outer diameter of the needle 312 is too small, the structural strength will be low, and it will be easy to bend or even break.

[0042] Exemplarily, the inner diameter of the needle 312 may be between 0.1 mm and 0.3 mm. The inner diameter of the needle 312 may be, for example, 0.1 mm, 0.2 mm, or 0.3 mm. If the inner diameter of the needle 312 is too large, the structural strength may be low, which may cause bending or even breaking. If the inner diameter of the needle 312 is too small, the resistance to liquid aspiration and sample application may be too large, which may result in high energy consumption of the drive assembly 200.

[0043] For example, the operating needle 310 may be a corrosion-resistant needle, so that the operating needle 310 can aspirate and spot corrosive sample liquids, and the operating needle 310 has high applicability.

[0044] Exemplarily, the operating needle 310 may include multiple ones, including but not limited to the two shown in the figure, for example, three or more. The drive assembly 200 can drive multiple operating needles 310 so that each operating needle 310 can absorb the sample liquid in the sample bottle one-to-one, and when spotting, it can also spot the sample on the spotting plate one-to-one. With this arrangement, the sampling assembly 300 has a high working efficiency in aspirating and spotting. Multiple operating needles 310 can be arranged in a horizontal direction. Multiple operating needles 310 can correspond to a capping member 320, and the capping member 320 can press the bottle caps of multiple sample bottles at the same time. Alternatively, multiple operating needles 310 can correspond to the capping member 320 one-to-one, and each capping member 320 can press the bottle cap of its corresponding sample bottle.

[0045] For example, Figure 1 As shown, the sampling device may further include a first horizontal driving member 110 and a second horizontal driving member 120. The driving end of the first horizontal driving member 110 may be connected to the main body of the driving assembly 200. Under the drive of the first horizontal driving member 110, the driving assembly 200 and the sampling assembly 300 may move along the first horizontal direction. The main body of the second horizontal driving member 120 may be arranged on the mounting frame 100. The driving end of the second horizontal driving member 120 may be connected to the main body of the first horizontal driving member 110. Under the drive of the second horizontal driving member 120, the first horizontal driving member 110, the driving assembly 200 and the sampling assembly 300 may move along the second horizontal direction. The second horizontal direction may be perpendicular to the first horizontal direction. The first horizontal driving member 110 includes but is not limited to a motor, a cylinder or an electric cylinder. The second horizontal driving member 120 includes but is not limited to a motor, a cylinder or an electric cylinder. With the cooperation of the first horizontal driving member 110 and the second horizontal driving member 120, the driving assembly 200 and the sampling assembly 300 can move in the first horizontal direction and the second horizontal direction, so that they can move to any suitable position in the horizontal direction. Exemplarily, the first horizontal driving member 110 and the second horizontal driving member 120 can drive the sampling assembly 300 to move above the sample bottle so that the operating needle 310 can aspirate liquid. When the aspiration is completed, the first horizontal driving member 110 and the second horizontal driving member 120 can drive the sampling assembly 300 to move above the spotting plate so that the operating needle 310 can spot the spotting plate. Compared with the use of driving members such as manipulators, the first horizontal driving member 110 and the second horizontal driving member 120 can accurately control the position of the sampling assembly 300, thereby improving the accuracy of the aspiration and spotting of the operating needle 310.

[0046] For example, Figure 2-4As shown, the drive assembly 200 may include a first drive member 210, a second drive member 220 and a third drive member 230. The driving end of the first drive member 210 may be connected to the gland member 320 to drive the gland member 320 to rise and fall, so that the bottle cap of the sample bottle can be pressed and separated. The driving end of the second drive member 220 may be connected to the main body (including the needle tube 311 and / or the needle 312) of the operating needle 310 to drive the operating needle 310 to rise and fall as a whole, so that it can pass through the hole 321 and extend into and out of the sample bottle. The driving end of the third drive member 230 may be connected to the piston rod 313 of the operating needle 310 to drive the piston rod 313 to rise and fall, so that liquid aspiration and spotting can be performed. The first drive member 210 includes but is not limited to a motor, a cylinder or an electric cylinder. The second drive member 220 includes but is not limited to a motor, a cylinder or an electric cylinder. The third drive member 230 includes but is not limited to a motor, a cylinder or an electric cylinder. Specifically, the first driving member 210 can drive the capping member 320 to descend to press the bottle cap of the sample bottle. Then, the second driving member 220 can drive the operating needle 310 to descend so that the operating needle 310 can pass through the hole 321, thereby extending into the sample bottle. Then, the third driving member 230 can drive the piston rod 313 of the operating needle 310 to rise, so that the sample liquid in the sample bottle can be absorbed. When the operating needle 310 completes the liquid aspiration, the second driving member 220 can drive the operating needle 310 to rise, so that it can pass through the hole 321 and detach from the sample bottle. Then, the first driving member 210 can drive the capping member 320 to rise, so that it can detach from the bottle cap of the sample bottle. When spotting is required, the first driving member 210 and / or the second driving member 220 can drive the operating needle 310 to have an expected height difference with the spotting plate. Then, the third driving member 230 can drive the piston rod 313 of the operating needle 310 to descend, so that the sample liquid can be spotted on the spotting plate. With such arrangement, the structure of the driving assembly 200 is relatively simple and easy to manufacture.

[0047] When the operating needle 310 is aspirating, the distance it descends will determine the length of the needle 310 extending into the sample bottle, thereby determining the maximum amount of sample liquid that can be aspirated. In addition, the distance the piston rod 313 rises will determine the amount of sample liquid aspirated.

[0048] When the operating needle 310 is spotting, and the height difference between the operating needle 310 and the spotting plate is greater than the expected height difference, the sample liquid may not be spotted on the spotting plate, or the spotting effect may not be as expected. When the height difference between the operating needle 310 and the spotting plate is less than the expected height difference, the operating needle 310 may contact the spotting plate, causing damage to both. Therefore, by accurately controlling the position of the operating needle 310 so that the operating needle 310 has the expected height difference with the spotting plate, the spotting effect can be improved. In addition, the distance that the piston rod 313 descends will determine the amount of sample liquid spotted.

[0049] For example, Figure 1-4As shown, the main body of the first driving member 210 can be directly or indirectly arranged on the mounting frame 100. In the embodiment where the sampling device includes the first horizontal driving member 110 and the second horizontal driving member 120, the main body of the first driving member 210 can be arranged on the driving end of the first horizontal driving member 110. The driving end of the first driving member 210 can be connected to the main body of the second driving member 220, the main body of the third driving member 230 and the gland member 320, so as to drive the second driving member 220, the third driving member 230, the operating needle 310 and the gland member 320 to rise and fall synchronously. The driving end of the second driving member 220 can be connected to the main body of the third driving member 230 and the main body of the operating needle 310, so as to drive the third driving member 230 and the operating needle 310 to rise and fall synchronously. With such an arrangement, the first driving member 210 can simultaneously drive the second driving member 220, the third driving member 230, the operating needle 310 and the gland member 320 to descend to approach the sample bottle. After the gland 320 presses the bottle cap of the sample bottle, the second driver 220 can drive the third driver 230 and the operating needle 310 to descend so that the operating needle 310 can extend into the sample bottle. Then, the third driver 230 can drive the piston rod 313 of the operating needle 310 to rise, so that the sample liquid in the sample bottle can be sucked. After the operating needle 310 has completed the liquid aspiration, the second driver 220 can drive the third driver 230 and the operating needle 310 to rise, so that the operating needle 310 can be separated from the sample bottle. Then, the first driver 210 can drive the second driver 220, the third driver 230, the operating needle 310 and the gland 320 to rise, so that the gland 320 can be separated from the bottle cap of the sample bottle. In this way, the drive assembly 200 can achieve multi-stage drive, thereby reducing the waste of energy. In addition, the second driver 220 and the third driver 230 drive the operation needle 310 and the piston rod 313 to rise and fall respectively, which can be relatively short, so that it is convenient to accurately control the position of the operation needle 310 and the piston rod 313 by adopting a structure with higher precision, and then the amount of liquid suction can be accurately controlled. When spotting is required, the first driver 210 and the second driver 220 can choose whether to start according to the position of the spotting plate. Exemplarily, when the height difference between the operation needle 310 and the spotting plate is much greater than the expected height difference, the first driver 210 can simultaneously drive the second driver 220, the third driver 230, the operation needle 310 and the gland member 320 to descend. Then, the second driver 220 can drive the third driver 230 and the operation needle 310 to descend, so that the operation needle 310 has the expected height difference with the spotting plate. Then, the third driver 230 can drive the piston rod 313 of the operation needle 310 to descend, so that the sample liquid can be spotted to the spotting plate. When the height difference between the operating needle 310 and the spotting plate is slightly greater than the expected height difference, the first driving member 210 may not need to be activated. The second driving member 220 may drive the third driving member 230 and the operating needle 310 to descend, so that the operating needle 310 is close to the spotting plate.Then, the third driving member 230 can drive the piston rod 313 of the operating needle 310 to descend, so that the sample liquid can be spotted to the spot plate. Exemplarily, when the operating needle 310 has an expected height difference with the spot plate, the first driving member 210 and the second driving member 220 do not need to be started. The third driving member 230 can directly drive the piston rod 313 of the operating needle 310 to descend, so that the sample liquid can be spotted to the spot plate. Of course, if the height difference between the operating needle 310 and the spot plate is less than the expected height difference, the first driving member 210 and / or the second driving member 220 can also drive the operating needle 310 to rise, and finally make the operating needle 310 have an expected height difference with the spot plate.

[0050] For example, Figure 2-4 As shown, the driving assembly 200 may further include a first connecting frame 240, a second connecting frame 250, a third connecting frame 260, a first slide rail 270 and a second slide rail 280. The first connecting frame 240 may be connected between the mounting frame 100 and the main body of the first driving member 210. In an embodiment where the sampling device includes a first horizontal driving member 110 and a second horizontal driving member 120, the first connecting frame 240 may be connected between the driving end of the first horizontal driving member 110 and the main body of the first driving member 210. The first slide rail 270 may be disposed on the first connecting frame 240. The second connecting frame 250 may be connected between the driving end of the first driving member 210 and the main body of the second driving member 220. Furthermore, the second connecting frame 250 may be slidably connected to the first slide rail 270. The gland member 320 and the second slide rail 280 may be disposed on the second connecting frame 250. The third connecting frame 260 may be connected between the driving end of the second driving member 220 and the main body of the third driving member 230. Furthermore, the third connecting frame 260 is slidably connected to the second slide rail 280. The main body of the operating pin 310 can be disposed on the third connecting frame 260. By disposing the first slide rail 270 and the second slide rail 280, the stability and linearity of the lifting and lowering of the pressing cover member 320 and the operating pin 310 can be improved.

[0051] For example, Figure 2-4 As shown, the sampling device may further include an operating needle base 331 and an operating needle cover plate 332. The operating needle base 331 may be disposed on the third connecting frame 260. The operating needle cover plate 332 may be detachably connected to the operating needle base 331 by any suitable means such as a connector connection or a snap connection to clamp the main body of the operating needle 310 (e.g., the needle tube 311 of the operating needle 310). With such a configuration, the operating needle 310 is easy to install and disassemble, thereby facilitating overall replacement.

[0052] For example, Figure 2-4As shown, the drive assembly 200 may further include a first lead screw 291 and a first nut 292. The drive end of the second drive member 220 may be connected to the first lead screw 291 to drive the first lead screw 291 to rotate. The first nut 292 may be connected to the third connecting frame 260. Furthermore, the first lead screw 291 may be threadedly connected to the first lead screw 291. The second drive member 220 may drive the first lead screw 291 to rotate, thereby driving the first nut 292 to rise and fall along the extension direction of the first lead screw 291, and then driving the third connecting frame 260 and the operating needle 310 connected to the third connecting frame 260 to rise and fall. By setting the first lead screw 291 and the first nut 292, the position of the operating needle 310 may be accurately controlled, and the stability of the lifting of the operating needle 310 is high. In this way, the precision of the liquid aspiration and spotting of the operating needle 310 is high.

[0053] For example, Figure 2-4 As shown, the drive assembly 200 may also include a second lead screw 293 and a second nut 294. The driving end of the third drive member 230 may be connected to the second lead screw 293 to drive the second lead screw 293 to rotate. The second nut 294 may be connected to the piston rod 313 of the operating needle 310 directly or through other components. In addition, the second nut 294 may be threadedly connected to the second lead screw 293. The third drive member 230 may drive the second lead screw 293 to rotate, thereby driving the piston rod 313 to rise and fall. By setting the second lead screw 293 and the second nut 294, the position of the piston rod 313 may be accurately controlled, and the stability of the lifting and lowering of the piston rod 313 is high. In this way, the precision of the operating needle 310 in aspirating and spotting liquid is high.

[0054] For some sample bottles, the bottle caps are usually provided with through holes. The through holes may be covered with a sealing layer to prevent the sample liquid in the sample bottle from volatilizing or spilling during transportation. The structure of the sealing layer may be arbitrary. In some embodiments, the sealing layer may be made of elastic materials such as silicone. The sealing layer may be provided with a gap in the shape of a letter or a cross. Generally, the sealing layer can close the gap by its own elasticity.

[0055] For example, Figure 2 and 5As shown, the gland member 320 may include a gland plate 324 and a flared boss 322. The flared boss 322 may protrude downward from the gland plate 324. The perforation hole 321 may successively penetrate the gland plate 324 and the flared boss 322. The drive assembly 200 may be connected to the gland plate 324 and / or the flared boss 322. The drive assembly 200 may be used to drive the gland plate 324 to press and disengage the bottle cap. Furthermore, the drive assembly 200 may be used to drive the flared boss 322 to flare the sealing layer on the bottle cap to separate the sealing layer from the operating pin 310. Specifically, as the drive assembly 200 drives the gland member 320 to descend, the flared boss 322 may be driven to flare the sealing layer, and then the gland plate 324 may press the bottle cap. Since the operating needle 310 passes through the hole 321, under the action of the expansion boss 322, the operating needle 310 will not contact the sealing layer, so that the structure of the sealing layer will not be damaged due to the expansion. In particular, for the above-mentioned sealing layer with a gap, if the expansion boss 322 is not provided, when the operating needle 310 expands the gap, the sealing layer will always be against the operating needle 310 due to the effect of elasticity. In this way, when the operating needle 310 rises and leaves the sample bottle, the sealing layer will scrape off the sample liquid on the operating needle 310, resulting in a reduction in the sample liquid that is already absorbed, and then resulting in a decrease in the effect of spotting. By providing the expansion boss 322, the sealing layer will not contact the operating needle 310, and the operating needle 310 can absorb the expected amount of sample liquid.

[0056] For example, Figure 5 As shown, the expansion boss 322 may be in a tapered shape that tapers downward. In this way, the area of ​​the lower end of the expansion boss 322 is small, so that the pressure acting on the sealing layer can be increased, which is conducive to expanding the sealing layer. Since the side of the expansion boss 322 is an inclined surface, the resistance when it leaves the sealing layer is small, thereby reducing the energy consumption of the drive assembly 200.

[0057] For example, Figure 5 As shown, the outer edge of the cover pressing plate 324 may have a positioning boss 323 protruding downward. Before the cover pressing plate 324 presses the bottle cap, the driving assembly 200 may drive the positioning boss 323 to preferentially sleeve the top of the bottle cap. The positioning boss 323 may be annular or arc-shaped. By providing the positioning boss 323, the bottle cap may be pre-positioned, so that the cover pressing plate 324 may accurately press the bottle cap of the sample bottle, and the operating needle 310 may accurately extend into the sample bottle.

[0058] For example, Figure 2-4As shown, the sampling device may further include an elastic assembly 400. The elastic assembly 400 may include a pressure rod 410 and an elastic member 420. The upper end of the elastic member 420 may be connected to the driving end of the drive assembly 200. In an embodiment in which the drive assembly 200 includes a first drive member 210, a second drive member 220 and a third drive member 230, the upper end of the elastic member 420 may be connected to the first drive member 210. The lower end of the elastic member 420 may be connected to the pressure rod 410. The drive assembly 200 may be used to drive the pressure rod 410 to rise and fall, thereby pressing and disengaging the bottle cap. The pressure rod 410 may protrude downward from the expansion boss 322. Under the drive of the drive assembly 200, the pressure rod 410 may press the bottle cap, and as it continues to decline, the pressure rod 410 may compress the elastic member 420, thereby relatively moving toward the expansion boss 322. As it continues to decline, the expansion boss 322 may expand the sealing layer. On the contrary, in the process that the drive assembly 200 drives the expanded boss 322 to rise and disengage from the sealing layer, the elastic member 420 can release elastic potential energy to drive the pressure rod 410 to move relative to the expanded boss 322, so that the bottle cap can be continuously pressed. If the elastic member 400 is not provided, the expanded boss 322 will rub against the sealing layer during the process of disengaging from the sealing layer, thereby driving the sample bottle to move. The elastic member 400 can make the pressure rod 410 continue to press the bottle cap to prevent the sample bottle from moving. With continued rise, when the pressure rod 410 is separated from the bottle cap, the elastic member 420 can completely release elastic potential energy, so that the pressure rod 410 can be driven to protrude downward from the expanded boss 322 for use next time. The elastic member 420 includes but is not limited to an elastic member made of elastic materials such as springs or silicone.

[0059] For example, Figure 2-4 As shown, the pressing rod 410 may include a pressing portion 411 and an extension rod 412. The extension rod 412 may extend upward from the pressing portion 411. The elastic member 420 may be sleeved on the extension rod 412. Furthermore, the lower end of the elastic member 420 may be connected to the pressing portion 411. The pressing portion 411 may be used to press and disengage the bottle cap. With such an arrangement, the structure of the elastic assembly 400 is relatively compact and occupies less space. Furthermore, the extension rod 412 may also guide the elastic member 420 to prevent the elastic member 420 from disengaging from the expected position.

[0060] For example, Figure 5 As shown, a pressure rod through hole 325 may be provided on the pressure cover plate 324. The extension rod 412 may pass through the pressure rod through hole 325. With such a configuration, the structure of the pressure cover member 320 and the elastic component 400 is relatively compact and occupies less space. In addition, the pressure rod through hole 325 may also guide the extension rod 412 to prevent the extension rod 412 from being out of the expected position.

[0061] Exemplarily, the spotting device may further include a spotting platform and a positioning assembly. A groove may be provided on the spotting platform. The groove may be used to place a spotting plate. The positioning assembly may be used to position the spotting plate in the groove. The structure of the positioning assembly may be arbitrary, for example, it may be a suction cup. In some embodiments, the positioning assembly may include a plurality of cylinder driving members, and the plurality of cylinder driving members may drive the spotting plate in the groove to move so as to position the spotting plate. The driving assembly 200 may be used to drive the operating needle 310 to spot the absorbed sample liquid onto the spotting plate. With such a configuration, the spotting device may also perform spotting operations, and its functions are more abundant.

[0062] Exemplarily, the spotting device may further include a detector and a controller. The controller may be electrically connected to the detector. The detector is located on the side of the spotting plate in the groove. In addition, the detector may be used to detect the height of the non-transparent top surface of the spotting plate at an angle downward to generate and send a signal to the controller. Generally speaking, the spotting plate may include a transparent body at the bottom and a coating on the top of the transparent body. The transparent body may be made of glass or other materials to facilitate photography. The coating may include a silicone coating or the like. The top surface of the coating may be a non-transparent top surface. The developing agent and the sample liquid may be attached to the non-transparent top surface. When the driving component 200 drives the operating needle 310 to spot, the controller may be used to make the operating needle 310 have an expected height difference with the non-transparent top surface based on the signal. The detector may use various types of detectors known in the art or that may appear in the future, including but not limited to laser sensors or infrared sensors. The controller can be built with electronic components such as timers, comparators, registers, digital logic circuits, etc., or it can be implemented using processor chips such as single-chip microcomputers, microprocessors, programmable logic controllers (PLCs), digital signal processors (DSPs), field programmable gate arrays (FPGAs), programmable logic arrays (PLA), application-specific integrated circuits (ASICs), and their peripheral circuits.

[0063] In actual application of the spotting device, the detector can tilt downward to send the detection light, and the detection light can be reflected back to the detector through the non-transparent top surface. In this way, the detector can send a signal to the controller. The controller can receive the signal, so that the height of the non-transparent top surface can be calculated. Exemplarily, the controller can use the distance from the non-transparent top surface of the standard spotting plate to the detector as the standard distance, and then can calculate the distance from the currently detected spotting plate to the detector based on the signal, and then can calculate the height of the non-transparent top surface of the currently detected spotting plate by comparison with the standard distance. In this way, when the driving component 200 drives the operating needle 310 to spot, the controller can control the distance of the operating needle 310 rising and falling based on the height of the non-transparent top surface, and then can make the operating needle 310 have an expected height difference with the non-transparent top surface. In this way, the spotting effect can be improved.

[0064] Due to manufacturing errors, the heights of different spotting plates may be different. The height of the non-transparent top surface of the spotting plate is calculated by the detector and the controller, so that the spotting effect of the operating needle 310 can be better. In this way, the differences in the spotting plates will not lead to a worse spotting effect. In addition, since the detector is located on the side of the spotting plate in the groove, it can avoid the space above the spotting plate, so as to facilitate the spotting of the operating needle 310, as well as the placement and removal of the spotting plate.

[0065] Exemplarily, the spotting device may further include a reference check block. After the operation needle 310 is replaced, the drive assembly 200 may be used to drive the lower end of the operation needle 310 to contact the reference check block, so that the position of the operation needle 310 is used as the reference position. In this way, the drive assembly 200 may drive the operation needle 310 to rise and fall at the reference position. In this way, even if there is an installation error when the operation needle 310 is replaced, the position of the operation needle 310 can still be accurately controlled.

[0066] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by directional words such as "front", "back", "up", "down", "left", "right", "lateral", "vertical", "vertical", "horizontal", "top", "bottom", etc. is usually based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention; the directional words "inside" and "outside" refer to the inside and outside relative to the contour of each component itself.

[0067] For ease of description, regional relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the regional positional relationship between one or more components or features shown in the figure and other components or features. It should be understood that regional relative terms include not only the orientation of the components as described in the figure, but also different orientations in use or operation. For example, if the components in the accompanying drawings are inverted as a whole, the components "above other components or features" or "above other components or features" will include the situation where the components are "below other components or structures" or "below other components or structures". Therefore, the exemplary term "above" may include both "above" and "below". In addition, these components or features may also be positioned at other different angles (e.g., rotated 90 degrees or other angles), and this article is intended to include all of these situations.

[0068] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, parts, components and / or combinations thereof.

[0069] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0070] The present invention has been described by the above embodiments, but it should be understood that the above embodiments are only for the purpose of example and description, and are not intended to limit the present invention to the scope of the described embodiments. In addition, it can be understood by those skilled in the art that the present invention is not limited to the above embodiments, and more variations and modifications can be made according to the teachings of the present invention, and these variations and modifications all fall within the scope of the protection claimed by the present invention. The protection scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A sampling device, characterized in that: include: Mounting frame; A driving assembly, the main body of which is arranged on the mounting frame; as well as A sampling component, the sampling component includes an operating needle connected to the driving end of the driving component and a pressure cover member with a penetration hole, the operating needle is at least used for sampling and can be raised and lowered along the penetration hole, the driving component is configured to: after driving the pressure cover member to press the bottle cap of the sample bottle, drive the operating needle to pass through the penetration hole and extend into the sample bottle to absorb the sample liquid in the sample bottle, and after driving the operating needle that has completed the liquid absorption to pass through the penetration hole and detach from the sample bottle, drive the pressure cover member to detach from the bottle cap.

2. The sampling device according to claim 1, characterized in that The driving assembly includes a first driving member, a second driving member and a third driving member. The driving end of the first driving member is connected to the capping member to drive the capping member to press and detach from the bottle cap. The driving end of the second driving member is connected to the main body of the operating needle to drive the operating needle to pass through the outlet hole and extend into and detach from the sample bottle. The driving end of the third driving member is connected to the piston rod of the operating needle to drive the operating needle to aspirate liquid.

3. The sampling device according to claim 2, characterized in that: The main body of the first driving member is arranged on the mounting frame, and the driving end of the first driving member is connected to the main body of the second driving member, the main body of the third driving member and the pressure cover member, so as to drive the second driving member, the third driving member, the operating needle and the pressure cover member to rise and fall synchronously, and the driving end of the second driving member is connected to the main body of the third driving member and the main body of the operating needle, so as to drive the third driving member and the operating needle to rise and fall synchronously.

4. The sampling device according to claim 3, characterized in that: The driving assembly also includes a first connecting frame, a second connecting frame, a third connecting frame, a first slide rail and a second slide rail. The first connecting frame is connected between the mounting frame and the main body of the first driving member, the first slide rail is arranged on the first connecting frame, the second connecting frame is connected between the driving end of the first driving member and the main body of the second driving member, and is slidably connected to the first slide rail, the pressure cover member and the second slide rail are arranged on the second connecting frame, the third connecting frame is connected between the driving end of the second driving member and the main body of the third driving member, and is slidably connected to the second slide rail, and the main body of the operating needle is arranged on the third connecting frame.

5. The sampling device according to claim 1, characterized in that: The cover pressing member includes a cover pressing plate and an expansion boss protruding downward from the cover pressing plate, the through hole passes through the cover pressing plate and the expansion boss, the driving assembly is used to drive the cover pressing plate to press and disengage from the bottle cap, and the expansion boss is used to expand the sealing layer on the bottle cap to separate the sealing layer from the operating needle.

6. The sampling device according to claim 5, characterized in that The expanded boss is in a tapered shape that tapers downward.

7. The sampling device according to claim 5, characterized in that: A positioning boss is protruded downward from the outer edge of the cover pressing plate, and the positioning boss is sleeved on the top of the bottle cap before the cover pressing plate presses the bottle cap.

8. The sampling device according to claim 5, characterized in that: The sampling device also includes an elastic component, which includes a pressure rod and an elastic member. The upper end of the elastic member is connected to the driving end of the driving component and the lower end is connected to the pressure rod. The driving component is used to drive the pressure rod to press and disengage from the bottle cap. When the expansion boss expands the sealing layer, the pressure rod presses the bottle cap to compress the elastic member and make the elastic member have elastic potential energy to drive the pressure rod to protrude downward from the expansion boss.

9. The sampling device according to claim 8, characterized in that The pressing rod comprises a pressing portion and an extending rod extending upward from the pressing portion, and the elastic member is sleeved on the extending rod and has a lower end connected to the pressing portion.

10. The sampling device according to claim 1, characterized in that: The operating needle includes a needle tube, a needle and a piston rod. The driving end of the driving assembly is connected to the piston rod to drive the piston rod to rise and fall in the needle tube. The needle is integrally connected to the lower end of the needle tube.

11. The sampling device according to claim 10, characterized in that: The outer diameter of the needle is between 0.4 mm and 0.6 mm; and / or The inner diameter of the needle is between 0.1 mm and 0.3 mm.

12. A spotting device, characterized in that: It comprises the sampling device as described in any one of claims 1 to 11, the spotting device also comprises a spotting table for placing a spotting plate, and the driving component is also used to drive the operating needle to spot the sucked sample liquid onto the spotting plate.

13. The spotting device according to claim 12, characterized in that: The spotting device further comprises a positioning component. A groove for placing the spotting plate is arranged on the spotting table, and the positioning component is used for positioning the spotting plate in the groove.

14. The spotting device according to claim 13, characterized in that: The spotting device also includes a detector and a controller electrically connected to the detector. The detector is located at the side of the spotting plate and is used to detect the height of the non-transparent top surface of the spotting plate at an angle downward to generate and send a signal to the controller. The controller is used to make the operating needle have an expected height difference with the non-transparent top surface when the driving component drives the operating needle to spot based on the signal.

15. The spotting device according to claim 12, characterized in that: The spotting device further comprises a reference check block, and the driving assembly is further used to drive the lower end of the operating needle to contact the reference check block, so that the position of the operating needle is used as a reference position.

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