A green muscardine algal glue bait granule for controlling bamboo forest click beetles and a preparation method thereof

Abstract

The present application discloses a kind of green algae glue for controlling bamboo forest gold needleworm of Metarhizium and preparation method, and each 100ml water contains the following weight components: 1-3g of sodium alginate, 0.7-0.8g of Metarhizium spore powder, 1-3g of yeast, 7-9g of attapulgite, 0.3-0.7g of rice flour.The green algae glue for Metarhizium prepared by the present application is safe to human and other organisms, has long-lasting control effect, is non-toxic and non-residual, has strong specificity in pest control, is easy to coordinate with its control measures, can save energy, and can realize green management of bamboo industry.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological pesticides, and particularly relates to a green muscadine fungus alginate bait granule for controlling bamboo forest wireworms and a preparation method. BACKGROUND

[0002] Wireworms are the larvae of Elateridae (Coleoptera Polyphaga Elateridae), which harm various crops, trees, Chinese medicinal materials and pasture, and mainly feed on the underground parts of plants. Wireworms have a long life cycle, long underground activity and damage time, and strong concealment, and the activity depth changes with the change of the external environment.

[0003] Wireworms are one of the most important pests in bamboo shoot season in southern regions, causing serious damage to the underground parts of bamboo. Wireworms feed on bamboo shoots during the bamboo shoot season, which not only reduces the market value of bamboo shoots, but also causes the death of bamboo shoots, thereby seriously restricting the renewal of bamboo forests. Therefore, the control of bamboo forest wireworms has become an urgent problem in production.

[0004] Biological control has the advantages of environmental friendliness, safety to humans and other organisms, long-lasting control effect, no toxicity and no residue, strong specificity for pest control, easy coordination with other control measures, and energy saving. With the enhancement of people's environmental protection consciousness and the increase of food safety requirements, biological control plays an increasingly important role in integrated pest control.

[0005] Biological control has received high attention in recent years. Muscadine fungus is one of the most studied and applied entomogenous fungi in the world. Various muscadine fungus preparations for grassland locusts and grubs have been developed at home and abroad. However, there are few reports on the pathogenicity and control of muscadine fungus on wireworms. In recent years, Kabuluk et al. systematically studied the control effect of muscadine fungus on Agriotes obscurus. The results of field tests showed that the muscadine fungus had obvious toxic effect. However, the indoor pathogenicity test of the strain on bamboo forest wireworms in China showed no obvious effect. Muscadine fungus has strong specificity for its host. Therefore, it is crucial to isolate a biocontrol strain with strong pathogenicity against bamboo forest wireworms in China, which is the basis and key to the biological control of wireworms. SUMMARY

[0006] In view of the problems in the prior art, the application is designed to provide a green muscadine fungus alginate bait granule for controlling bamboo forest wireworms and a preparation method, which is realized by the following technical scheme:

[0007] A kind of green muscardine algae glue bait granules for controlling bamboo forest gold needleworm, containing the following components by weight per 100ml water: 1-3g of sodium alginate, 0.7-0.8g of green muscardine spore powder, 1-3g of yeast, 7-9g of attapulgite, 0.3-0.7g of rice flour.

[0008] Further, containing the following components by weight per 100ml water: 2g of sodium alginate, 0.75g of green muscardine spore powder, 2g of yeast, 8g of attapulgite, 0.5g of rice flour.

[0009] Further, the green muscardine is Metarhizium pingshaense WP08 strain Metarhizium pingshaense, and the preservation number is CGMCC No.22474.

[0010] Further, the attapulgite is selected from 1250 mesh attapulgite.

[0011] The preparation method of the green muscardine algae glue bait granules for controlling bamboo forest gold needleworm, the preparation method comprises the following steps:

[0012] 1) 1-3g of sodium alginate, 0.7-0.8g of green muscardine spore powder, 1-3g of yeast, 7-9g of attapulgite, 0.3-0.7g of rice flour are sequentially added into 100ml water, and mixed and stirred uniformly to obtain a stirring liquid;

[0013] 2) the stirring liquid is sucked by using a micro-injection pump and dropped into a 0.2 mol / L calcium chloride solution drop by drop, the particle size is controlled to be 6mm, the algae glue granules are formed, after being placed for 15-25min, the drying time is 2-3d.

[0014] Further, the flow rate of the micro-injection pump is 400 mL / h.

[0015] Further, in step 2), the standing time is 15-25min, and the drying time is 2-3d.

[0016] The green muscardine algae glue bait granules prepared by the method have the advantages of safety to human and other organisms, long-lasting control effect, no toxicity and no residue, strong specificity in pest control, easy coordination with its control measures, energy saving, and realization of green management of bamboo industry. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a rate effect diagram for green muscardine algae glue granules for controlling bamboo forest gold needleworm in the forest;

[0018] Figure 2 It is a rate effect diagram for green muscardine algae glue granules for controlling bamboo forest gold needleworm in the forest;

[0019] Figure 3 Comparison of the effects of Metarhizium anisopliae algae granules on wireworm shoot pruning in forests. Detailed Implementation

[0020] The present invention will be further described below with reference to specific embodiments in order to better understand the technical solution.

[0021] Example: Preparation of Metarhizium anisopliae alginate granules

[0022] Prepare the necessary spore powder, carrier, and nutrient activator for the experiment. Add 0.75% Metarhizium anisopliae spore powder, 2% yeast, 8% 1250 mesh attapulgite clay, and 0.5% rice flour to a 2% sodium alginate solution. Then, use a micro-injection pump (LINZ-9A, flow rate 400 mL / h) to draw up the stirred liquid and dropwise add it to a 0.2 mol / L calcium chloride solution, controlling the particle size to 6 mm to form alginate particles. After standing for 20 min to solidify, wash back and forth with clean water for 2-3 min, filter, and place on a clean tray to dry for 2-3 days to form dried Metarhizium anisopliae alginate granules.

[0023] Preparation of yeast granules: The preparation method is the same as that of Metarhizium anisopliae alginate attractant granules, the only difference being that Metarhizium anisopliae spore powder is not added during the preparation process.

[0024] Preparation of Metarhizium anisopliae granules: The preparation method is the same as that of Metarhizium anisopliae alginate attractant granules, the only difference being that yeast is not added during the preparation process.

[0025] Experimental Example 1: Sporulation Rate of Three Types of Granules

[0026] Sporulation rates of the three granules were measured under the same conditions, and the results are shown in Table 1.

[0027] Table 1: Sporulation yield of three types of granules

[0028]

[0029] Experimental Example 2: Selective Behavior of Wireworms Towards Three Granular Formulations

[0030] Fill the two-way selector with 1.75 kg of sterile soil with a moisture content of 10%, and shake the wooden box horizontally to make the soil surface level and the soil texture uniform in all parts, and set it aside.

[0031] Twenty granules of each of the yeast granules, Metarhizium granules, and Metarhizium alginate bait granules were buried in the bait chambers on either side of the two-way selection instrument, and the other side of the bait chamber was left as a control. At the same time, 10 heads of the test golden needleworm were placed in the sterile soil in the center of the worm chamber, and the partition was removed. After 24 hours, the previously removed partition was reassembled. First, the number of golden needleworms in the center of the worm chamber was counted (marked as "no selection"), and then the number of golden needleworms contained in the two bait chambers was counted in turn. The test was carried out in the dark (room temperature 25 ± 1°C, relative humidity 80% ± 10%), and each treatment had 12 replicates. The comparison of the attraction effects of the three granules on the golden needleworm is shown in Table 2.

[0032] Table 2: Comparison of the attraction effects of the three granules on the golden needleworm

[0033]

[0034] The results showed that the total number of C. chinensis larvae captured in the bait chamber of the Metarhizium alginate bait granules was 52, accounting for 66.67% of the selected insects, with an average of 4.33 ± 0.38 captured per bait chamber (GP = 8.83, P < 0.005; GH = 8.11, P = 0.703); the total number of C. chinensis larvae captured in the bait chamber of the yeast granules was 53, accounting for 72.60% of the selected insects, with an average of 4.42 ± 0.49 captured per bait chamber (GP = 15.47, P < 0.001; GH = 23.06, P = 0.017); the total number of C. chinensis larvae captured in the bait chamber of the Metarhizium granules was 27, accounting for 38.57% of the selected insects, with an average of 2.25 ± 0.33 captured per bait chamber (GP = 3.69, P > 0.05; GH = 20.43, P = 0.039); the selection behavior of C. chinensis larvae was significantly affected by the Metarhizium alginate bait granules and the yeast granules.

[0035] Test Example 3: Field application of Metarhizium alginate bait granules

[0036] The application method used ditch application, with a ditch width of 20 cm, a ditch spacing of 80-100 cm, and a ditch depth of 20-30 cm. The bait granules were applied into the ditch in early May and late October to early November, with a single application amount of 20 kg / hm2 to 40 kg / hm2, and covered with soil. The demonstration results showed that the comprehensive control technology significantly controlled the golden needleworm in bamboo forests in each demonstration area. For example, in the Fuyang demonstration area, after two years of continuous management, the golden needleworm damage rate in the Fuyang demonstration area decreased from 65.24% to 8.20% (as shown in Figure 1 ), the bamboo shoot yield increased by 15.38%, and the population density decreased from 0.93 per plant to 0.42 per plant (as shown in Figure 2 ). The control effect was Figure 3The results showed that the damage of the gold needle worm was controlled in the demonstration area, and the normal renewal of bamboo forest was ensured.

Claims

1. A Metarhizium albugo alginate-bait granule for controlling bamboo armyworm, characterized in that The following components are contained in 100ml of water: 1-3g of sodium alginate, 0.7-0.8g of Metarhizium anisopliae spore powder, 1-3g of yeast, 7-9g of attapulgite, and 0.3-0.7g of rice powder; The granules are prepared by the following method: 1) 1-3g of sodium alginate, 0.7-0.8g of Metarhizium anisopliae spore powder, 1-3g of yeast, 7-9g of attapulgite, and 0.3-0.7g of rice powder are sequentially added into 100ml of water and mixed and stirred uniformly to obtain a stirring liquid; 2) the stirring liquid is sucked by a micro-injection pump and dropped into a 0.2 mol / L calcium chloride solution drop by drop, the particle size is controlled to be 6mm, and Metarhizium anisopliae alginate granules are formed; after being solidified and formed, the Metarhizium anisopliae alginate granules are washed with water for 2-3min, filtered, and placed in a clean tray for drying to obtain Metarhizium anisopliae alginate granules; Metarhizium anisopliae is Metarhizium planum WP08 strain Metarhizium pingshaense with the preservation number of CGMCC No.22474; The attapulgite is 1250-mesh attapulgite.

2. The Metarhizium alveatum alginate-bait granule for controlling the bamboo borer as claimed in claim 1, wherein the Metarhizium alveatum is Metarhizium anisopliae var. anisopliae. The following components are contained in 100ml of water: 2g of sodium alginate, 0.75g of Metarhizium anisopliae spore powder, 2g of yeast, 8g of attapulgite, and 0.5g of rice powder.

3. The Metarhizium alveatum alginate-bait granule for controlling bamboo borer as claimed in claim 1, wherein the Metarhizium alveatum is Metarhizium anisopliae var. anisopliae. The flow rate of the micro-injection pump is 400 mL / h.

4. The Metarhizium alveatum alginate-bait granule for controlling bamboo borer as claimed in claim 1, wherein the Metarhizium alveatum is Metarhizium anisopliae var. acridum. In step 2), the Metarhizium anisopliae alginate granules are placed for 15-25min, and the drying time is 2-3d.

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