A kind of blood lipid lowering tea bag and preparation method thereof

Abstract

The invention provides a blood lipid lowering tea bag and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The blood lipid lowering tea bag of the invention comprises: hawthorn, gynostemma pentaphyllum, bu za leaf, honeysuckle, kuding tea, pollen and liquorice. Among them, hawthorn can help digestion and invigorate the stomach, promote the digestion and decomposition of fat, gynostemma pentaphyllum can clear turbidity and reduce lipid, inhibit the differentiation of adipocytes and the synthesis of lipids, and they are all monarch drugs; bu za leaf can help digestion and resolve stagnation, and honeysuckle can clear heat and detoxify, and they are minister drugs; kuding tea can disperse wind and heat, clear the head, regulate body fluid metabolism, pollen can remove blood stasis and stop bleeding, and improve blood circulation, and they are adjuvant drugs; liquorice can harmonize various drugs and is a guiding drug. Various drugs are used together to synergistically achieve the effect of lowering blood lipids. The results of animal experiments show that taking the tea bag of the invention can significantly reduce the cholesterol, triglyceride and low-density lipoprotein cholesterol levels of mice, and increase the high-density lipoprotein cholesterol level, indicating that the tea bag of the invention has an excellent blood lipid lowering effect.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a blood lipid-lowering tea bag and a preparation method thereof. Background Art

[0002] With the changes in modern lifestyles, including the influence of high-calorie diets, lack of exercise, and long-term mental stress, the incidence of hyperlipidemia has shown a significant upward trend worldwide. Hyperlipidemia refers to a metabolic disorder syndrome in which the levels of lipid components in the blood, such as cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), are elevated, and the levels of high-density lipoprotein cholesterol (HDL-C) are reduced. Long-term high blood lipid status can lead to the formation of atherosclerosis, causing the blood vessel walls to thicken, harden, and lose elasticity, which in turn leads to a series of serious cardiovascular and cerebrovascular diseases, such as coronary heart disease, myocardial infarction, and stroke. These diseases not only seriously affect the quality of life of patients, but also have a high disability rate and mortality rate, bringing a heavy medical burden to families and society. According to relevant statistics, among the people who died from cardiovascular diseases, more than 70% are closely related to hyperlipidemia.

[0003] At present, commonly used lipid-lowering drugs mainly include chemical synthetic drugs such as statins, fibrates, and niacin. Statins are the most widely used cholesterol-lowering drugs. They lower blood cholesterol levels by inhibiting key enzymes in the cholesterol synthesis process. Although their lipid-lowering effects are significant, long-term use may cause some adverse reactions, such as liver damage, rhabdomyolysis, etc., and some patients are intolerant to statins. Fibrates are mainly used to lower triglyceride levels, but they may also cause side effects such as gastrointestinal discomfort and abnormal liver and kidney function. While niacin drugs lower blood lipids, they are often accompanied by adverse reactions such as facial flushing, skin itching, and hyperglycemia, which limits their widespread clinical application.

[0004] Traditional Chinese medicine has a long history and rich experience in treating metabolic diseases. Traditional Chinese medicine is relatively safe, has fewer adverse reactions, and is more suitable for long-term use. The development of an efficient and safe lipid-lowering traditional Chinese medicine composition has broad market prospects and important significance, and is expected to provide a new treatment option for patients with hyperlipidemia and meet the growing medical needs. Summary of the invention

[0005] In view of the above technical problems, the present invention provides a blood lipid lowering tea bag and a preparation method thereof. The tea bag provided by the present invention has an excellent blood lipid lowering effect.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: The invention provides a blood lipid lowering Chinese medicinal composition, comprising the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of buzia leaves, 20-30 parts of honeysuckle, 3-8 parts of kudingcha tea, 1-3 parts of pollen cattail and 1-3 parts of liquorice.

[0007] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of blood lipid lowering drugs.

[0008] The invention also provides a tea bag for lowering blood lipids, which is made from the above-mentioned Chinese medicine composition.

[0009] The present invention also provides a preparation method of the above-mentioned blood lipid-lowering tea bag, comprising: respectively crushing hawthorn, gynostemma pentaphyllum, buzia leaf, honeysuckle, kudingcha and licorice; mixing hawthorn and gynostemma pentaphyllum with water, enzymolysis with hemicellulase and nattokinase in turn, fermenting with composite microorganisms, sterilizing, filtering, and obtaining fermentation liquid; mixing buzia leaf and honeysuckle, ultrasonically extracting with ethanol solution, filtering, and obtaining filtrate; mixing the fermentation liquid and the filtrate, and spray-drying to obtain dry powder; mixing kudingcha, pollen pollen, licorice and the dry powder, sterilizing, and putting into tea bags to obtain the blood lipid-lowering tea bag; the composite microorganisms are composed of purple red yeast rice and schizophyllum in a weight ratio of 1-3:2-6.

[0010] Preferably, the ratio of the total weight of the hawthorn and Gynostemma pentaphyllum to the weight of water is 1:5-8.

[0011] Preferably, the amount of hemicellulase added is 0.5‰-1.3‰ of the total weight of hawthorn and Gynostemma pentaphyllum, the temperature of hemicellulase enzymolysis is 50-60°C, the pH value is 4-6, and the time is 1-3h; the amount of nattokinase added is 1‰-3‰ of the total weight of hawthorn and Gynostemma pentaphyllum, the temperature of nattokinase enzymolysis is 45-55°C, the pH value is 7-9, and the time is 2-4h.

[0012] Preferably, the inoculation amount of the composite microorganism is 4‰-8‰ of the total weight of hawthorn and Gynostemma pentaphyllum, the fermentation temperature is 25-35°C, the pH value is 5-6.5, and the fermentation time is 5-10 days.

[0013] Preferably, the volume percentage of the ethanol solution is 45%-65%, and the mass ratio of the total mass of the Buza leaves and honeysuckle to the ethanol solution is 1:5-8.

[0014] Preferably, the power of the ultrasound is 200-300W.

[0015] Preferably, the material-liquid ratio of the extraction is 1:5-8 g / ml, the temperature is 40-50° C., the time is 30-40 min, and the number of extractions is 2-4 times.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a blood lipid lowering Chinese medicine composition, comprising: hawthorn, gynostemma pentaphyllum, bu za leaf, honeysuckle, kudingcha, pollen typhae and liquorice. Among them, hawthorn can help digestion and invigorate the stomach, promote the digestion and decomposition of fat, gynostemma pentaphyllum can clear turbidity and reduce lipid, inhibit the differentiation of adipocytes and the synthesis of lipids, and they are all monarch drugs; bu za leaf can help digestion and resolve stagnation, and honeysuckle can clear heat and detoxify, and they are minister drugs; kudingcha can dissipate wind and heat, clear the head, and regulate body fluid metabolism, pollen typhae can remove blood stasis and stop bleeding, and improve blood circulation, and they are adjuvant drugs; liquorice can harmonize various drugs and is a guiding drug. All drugs are used together to synergistically achieve the effect of lowering blood lipids. DETAILED DESCRIPTION

[0017] The invention provides a blood lipid lowering Chinese medicinal composition, comprising the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of buzia leaves, 20-30 parts of honeysuckle, 3-8 parts of kudingcha tea, 1-3 parts of pollen cattail and 1-3 parts of liquorice.

[0018] The hawthorn of the present invention is good for digestion and stomach, contains a variety of organic acids, such as hawthorn acid, citric acid, etc., which can increase the secretion of digestive enzymes in the stomach and promote the digestion and decomposition of fat. At the same time, hawthorn can also regulate lipid metabolism and reduce the content of triglycerides and cholesterol in the blood. In the traditional Chinese medicine composition, hawthorn plays a leading and key role in regulating blood lipids and is the main drug.

[0019] The Gynostemma pentaphyllum described in the present invention can clear turbidity and reduce lipids, inhibit the differentiation of adipocytes and the synthesis of lipids, reduce low-density lipoprotein cholesterol (LDL-C), and increase high-density lipoprotein cholesterol (HDL-C), playing an important role in the entire blood lipid regulation process. In the traditional Chinese medicine composition, it serves as the main medicine together with hawthorn.

[0020] The Buza leaf of the present invention has the effect of digesting food and removing stagnation, and can assist the main drug Crataegus pinnatifida to strengthen the digestion of fat in food. The Buza leaf can inhibit cholesterol absorption, regulate lipid metabolism, and promote excretion, thereby indirectly playing a role in lowering blood lipids. In the traditional Chinese medicine composition, the Buza leaf is used as an assistant drug to assist the main drug Crataegus pinnatifida to enhance its effect of digesting food and lowering lipids.

[0021] The honeysuckle of the present invention can clear away heat and detoxify, and has antioxidant and anti-inflammatory properties. Oxidative stress and inflammatory reactions often occur in patients with hyperlipidemia, and these reactions interfere with lipid metabolism. Honeysuckle can alleviate inflammatory reactions and remove free radicals, thereby creating a good internal environment for the normal metabolism of blood lipids. In combination with the main drug, it assists in lowering blood lipids from the perspective of improving the overall state of the body, and plays the role of the minister drug.

[0022] The Kudingcha of the present invention can dissipate wind-heat, clear the head and eyes, and regulate body fluid metabolism. In the Chinese medicine composition, Kudingcha can prevent the dryness and heat that may be caused by other drugs (such as hawthorn, etc.) in the formula. At the same time, Kudingcha is beneficial to the cardiovascular system, can regulate blood lipids, and play an auxiliary role in lowering blood lipids. Therefore, it is an adjuvant, enhances the blood lipid-lowering effect of the entire formula, and harmonizes the medicinal properties.

[0023] The pollen of the present invention has the effect of removing blood stasis and stopping bleeding. In the case of abnormal blood lipids, blood viscosity increases and blood stasis is likely to occur. Pollen of the present invention can improve the stasis state of blood, promote blood circulation, and enable lipids in the blood to be better transported and metabolized. In the Chinese medicine composition, pollen of the present invention is used as an adjuvant to assist the main medicine and the ministerial medicine to improve blood circulation, thereby facilitating the regulation of blood lipids.

[0024] The licorice of the present invention has the effect of harmonizing various medicines, can alleviate the strongness of other medicines, and is a drug. For example, the efficacy of drugs such as hawthorn and gynostemma pentaphyllum is relatively strong, and licorice can make them better synergistic with each other and avoid possible conflicts between drugs. At the same time, licorice itself also has certain pharmacological effects, such as anti-inflammatory, anti-allergic, etc., which also help to improve the overall state of the body and are conducive to the regulation of blood lipids.

[0025] In the Chinese medicine composition of the present invention, hawthorn can help digestion and invigorate the stomach, promote the digestion and decomposition of fat, gynostemma pentaphyllum can clear turbidity and reduce lipids, inhibit the differentiation of fat cells and the synthesis of lipids, and they are all the main drugs; buza leaves can help digestion and resolve stagnation, and honeysuckle can clear away heat and detoxify, and they are the assistant drugs; kudingcha can disperse wind and heat, clear the head, and regulate body fluid metabolism, and pollen can remove blood stasis and stop bleeding, and improve blood circulation, and they are the adjuvant drugs; and liquorice can harmonize the drugs and is the guiding drug. The drugs are used together to synergistically achieve the effect of lowering blood lipids.

[0026] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of blood lipid lowering drugs.

[0027] The invention also provides a tea bag for lowering blood lipids, which is made from the above-mentioned Chinese medicine composition.

[0028] The present invention also provides a preparation method of the above-mentioned lipid-lowering tea bag, comprising: respectively crushing hawthorn, gynostemma pentaphyllum, buzia leaves, honeysuckle, kudingcha and licorice; mixing hawthorn and gynostemma pentaphyllum with water, enzymolysis with hemicellulase and nattokinase in turn, fermenting with composite microorganisms, filtering to obtain fermentation liquid; mixing buzia leaves and honeysuckle, ultrasonic extraction with ethanol solution, filtering to obtain filtrate; mixing the fermentation liquid and the filtrate, spray drying to obtain dry powder; mixing kudingcha, pollen pollen, licorice and the dry powder, and putting them into tea bags to obtain the lipid-lowering tea bag; the composite microorganisms are composed of purple red yeast rice and schizophyllum in a weight ratio of 1-3:2-6.

[0029] The weight ratio of the total weight of hawthorn and Gynostemma pentaphyllum to the weight of water is preferably 1:5-8, more preferably 1:6.

[0030] The enzymatic activity of the hemicellulase of the present invention is preferably 40,000-80,000 U / g, more preferably 50,000 U / g, and the added amount is preferably 0.5‰-1.3‰ of the total weight of hawthorn and Gynostemma pentaphyllum, more preferably 1‰, the temperature of the hemicellulase hydrolysis is preferably 50-60°C, more preferably 55°C, the pH value is preferably 4-6, more preferably 5, and the time is preferably 1-3h, more preferably 2h; the enzymatic activity of the nattokinase is preferably 10,000-30,000 U / g, more preferably 20,000 U / g, the added amount is preferably 1‰-3‰ of the total weight of hawthorn and Gynostemma pentaphyllum, more preferably 2‰, the temperature of the nattokinase hydrolysis is preferably 45-55°C, more preferably 50°C, the pH value is preferably 7-9, more preferably 8, and the time is preferably 2-4h, more preferably 3h. The invention firstly uses hemicellulase to enzymolyze hawthorn and gynostemma pentaphyllum to destroy the cell wall structure, promote the dissolution and transformation of effective components, and then uses nattokinase to enzymolyze to more significantly regulate blood lipid metabolism and synergistically achieve the effect of lowering blood lipids.

[0031] The purple red yeast rice in the composite microorganism of the present invention is purple red yeast rice Monascus purpureus CICC ® 5046, the bacterial activity is 3 billion to 8 billion CFU / g, more preferably 5 billion CFU / g; the Schizophyllum in the composite microorganism is Schizophyllum Schizophyllum commune CICC ® 2592, the bacterial activity is 8-12 billion CFU / g, more preferably 10 billion CFU / g. Purple red yeast rice can inhibit cholesterol synthesis, reduce triglycerides and low-density lipoprotein, improve lipid metabolism, and has the effect of lowering blood lipids; Schizophyllum fermentation can produce schizophyllum polysaccharide, which can inhibit the activity of fatty acid synthase and reduce the synthesis of fatty acids. The present invention ferments purple red yeast rice and schizophyllum together on hawthorn and gynostemma pentaphyllum, which has a synergistic effect and achieves a more significant blood lipid-lowering effect.

[0032] The composite microorganism of the present invention is preferably composed of purple red yeast rice and schizophyllum in a weight ratio of 1-3:2-6, more preferably 2:5; the inoculation amount of the composite microorganism is preferably 4‰-8‰ of the total weight of hawthorn and Gynostemma pentaphyllum, more preferably 5‰, the fermentation temperature is preferably 25-35°C, more preferably 30°C, the pH value is preferably 5-6.5, more preferably 6, and the time is preferably 5-10d, more preferably 8d.

[0033] The volume percentage of the ethanol solution of the present invention is preferably 45%-65%, more preferably 55%, the mass ratio of the total mass of the Buzha leaves and honeysuckle to the ethanol solution is preferably 1:5-8, more preferably 1:6, the power of the ultrasound is preferably 200-300W, more preferably 260W; the solid-liquid ratio of the extraction is preferably 1:5-8g / ml, more preferably 1:6g / ml, the temperature is preferably 40-50°C, more preferably 45°C, the time is preferably 30-40min, more preferably 35min, and the number of extractions is preferably 2-4 times, more preferably 3 times.

[0034] The invention carries out ethanol ultrasonic extraction on buza leaves and honeysuckle, which can accelerate the dissolution of effective ingredients in the traditional Chinese medicine and improve the effect of lowering blood lipids.

[0035] Each Chinese medicine tea bag contains 3g of tea and is brewed with boiling water once a day.

[0036] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0037] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0039] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0040] The purple red yeast rice involved in the embodiment is purple red yeast rice Monascus purpureus CICC ® 5046, Schizophyllum is Schizophyllum Schizophyllum commune CICC ® 2592.

[0041] Example 1 Preparation of blood lipid lowering tea bag (1) 75 parts by weight of hawthorn, 60 parts by weight of gynostemma pentaphyllum, 40 parts by weight of buzia leaf, 25 parts by weight of honeysuckle, 5 parts by weight of kudingcha tea and 2 parts by weight of liquorice were respectively ground into a particle size of 100 mesh; (2) Mix hawthorn, Gynostemma pentaphyllum and water (the weight of water is 6 times the total weight of hawthorn and Gynostemma pentaphyllum), add hemicellulase (enzyme activity of 50,000 U / g) at a total weight of 1‰ of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 55°C and pH 5 for 2 hours. Then, add nattokinase (enzyme activity of 20,000 U / g) at a total weight of 2‰ of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 50°C and pH 8 for 3 hours. Then, heat the mixture to 85°C to inactivate the enzyme for 15 minutes. After cooling to room temperature, inoculate the mixture with a composite microorganism (composed of purple red yeast rice with a bacterial activity of 5 billion CFU / g and schizophyllum with a bacterial activity of 10 billion CFU / g in a weight ratio of 2:5) at a total weight of 5‰ of hawthorn and Gynostemma pentaphyllum, and ferment at 30°C and pH 6 for 8 days. Sterilize the mixture and filter it to obtain a fermentation liquid. (3) Mix the leaves of Buzha and honeysuckle with 55% by volume ethanol solution (the weight of the ethanol solution is 6 times the total weight of the leaves of Buzha and honeysuckle), extract for 35 min at 260 W ultrasonic power and 45 ° C, filter, extract twice more according to the above steps, and combine the filtrates; (4) After mixing the fermentation broth and the filtrate, sterilize at 121°C for 15 min, and spray dry at 100°C to a water content of 4% to obtain a dry powder; (5) The dry powder, Kudingcha, liquorice and 2 parts by weight of Puhuang are mixed, sterilized and put into tea bags to obtain blood lipid lowering tea bags.

[0042] Example 2 Preparation of blood lipid lowering tea bag (1) 70 parts by weight of hawthorn, 50 parts by weight of gynostemma pentaphyllum, 35 parts by weight of buzia leaf, 20 parts by weight of honeysuckle, 3 parts by weight of kudingcha tea and 1 part by weight of liquorice were respectively ground into a particle size of 80 mesh; (2) Mix hawthorn, Gynostemma pentaphyllum and water (the weight of water is 5 times the total weight of hawthorn and Gynostemma pentaphyllum), add hemicellulase (enzyme activity is 40,000 U / g) at 0.5‰ of the total weight of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 50°C and pH 4 for 1 hour. Then, add nattokinase (enzyme activity is 10,000 U / g) at 1‰ of the total weight of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 45°C and pH 7 for 2 hours. Then, heat the mixture to 85°C to inactivate the enzyme for 15 minutes. After cooling to room temperature, inoculate the mixture with a composite microorganism (composed of purple red yeast rice with a bacterial activity of 3 billion CFU / g and schizophyllum with a bacterial activity of 8 billion CFU / g in a weight ratio of 1:2) at 4‰ of the total weight of hawthorn and Gynostemma pentaphyllum, and ferment at 25°C and pH 5 for 10 days. Sterilize the mixture and filter it to obtain a fermentation liquid. (3) Mix the leaves of Buzha and honeysuckle with a 45% by volume ethanol solution (the weight of the ethanol solution is 5 times the total weight of the leaves of Buzha and honeysuckle), extract for 40 min at 200 W ultrasonic power and 40 ° C, filter, extract once more according to the above steps, and combine the filtrates; (4) After mixing the fermentation broth and the filtrate, sterilize at 121°C for 15 min, and spray dry at 80°C to a water content of 8% to obtain a dry powder; (5) The dry powder, Kudingcha, liquorice and 1 part by weight of Puhuang are mixed, sterilized and put into tea bags to obtain blood lipid lowering tea bags.

[0043] Example 3 Preparation of blood lipid lowering tea bag (1) 80 parts by weight of hawthorn, 70 parts by weight of gynostemma pentaphyllum, 45 parts by weight of buzia leaf, 30 parts by weight of honeysuckle, 8 parts by weight of kudingcha tea and 3 parts by weight of liquorice were respectively ground into a particle size of 120 mesh; (2) Mix hawthorn, Gynostemma pentaphyllum and water (the weight of water is 8 times the total weight of hawthorn and Gynostemma pentaphyllum), add hemicellulase (enzyme activity is 80,000 U / g) at a total weight of 1.3‰ of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 60°C and pH 6 for 3 hours. Then, add nattokinase (enzyme activity is 30,000 U / g) at a total weight of 3‰ of hawthorn and Gynostemma pentaphyllum, and perform enzymolysis at 55°C and pH 9 for 4 hours. Then, heat the mixture to 85°C to inactivate the enzyme for 15 minutes. After cooling to room temperature, inoculate the mixture with a composite microorganism (composed of purple red yeast rice with a bacterial activity of 8 billion CFU / g and schizophyllum with a bacterial activity of 12 billion CFU / g in a weight ratio of 3:6) at a total weight of 8‰ of hawthorn and Gynostemma pentaphyllum, and ferment at 35°C and pH 6.5 for 5 days. Sterilize the mixture and filter it to obtain a fermentation liquid. (3) Mix the leaves of Buzha and honeysuckle with a 65% by volume ethanol solution (the weight of the ethanol solution is 8 times the total weight of the leaves of Buzha and honeysuckle), extract for 30 min at 300 W ultrasonic power and 50 ° C, filter, and extract three more times according to the above steps, and combine the filtrates; (4) After mixing the fermentation broth and the filtrate, sterilize at 121°C for 15 min, and spray dry at 110°C to a water content of 3% to obtain a dry powder; (5) The dry powder, Kudingcha, liquorice and 3 parts by weight of Puhuang are mixed, sterilized and put into tea bags to obtain blood lipid lowering tea bags.

[0044] Comparative Example 1 The specific implementation method is the same as that of Example 1, except that the enzymolysis order of hemicellulase and nattokinase is swapped.

[0045] Comparative Example 2 The specific implementation method is the same as Example 1, except that the composite microorganism is replaced by 5 billion CFU / g of Bacillus subtilis Bacillus subtilis CICC ® 10148 and bacterial activity is 10 billion CFU / g brewer's yeast Saccharomyces cerevisiae CICC ®1423 A composite microorganism composed in a weight ratio of 2:5.

[0046] Comparative Example 3 The specific implementation is the same as that of Example 1, except that the ethanol solution is replaced by an aqueous solution.

[0047] Comparative Example 4 The specific implementation is the same as that of Example 1, except that hawthorn and Gynostemma pentaphyllum are not added.

[0048] Comparative Example 5 The specific implementation is the same as that of Example 1, except that no Buza leaves and honeysuckle are added.

[0049] Experimental Example 1 Animal experiments 100 4-week-old Kunming mice, half male and half female, weighing 15-20g, were fed with adaptive basal feed for one week and then divided into 10 groups, 10 mice in each group. There was no significant difference in the number of males and females, weight, growth, etc. among the groups. During the experiment, mice in each group were free to eat and drink. The specific groups are shown in Table 1. The high-fat feed was 85% basal feed + 10% lard + 1% cholesterol + 4% protein powder (the percentage content is the mass percentage content).

[0050] Table 1 Feeding scheme of experimental groups Group plan Blank group Basic feed + 0.4mL normal saline gavage Model Group High-fat diet + 0.4 mL saline solution gavage Example 1 High-fat diet + 0.4g of the Chinese medicine composition of Example 1 / kg body weight gavage Example 2 High-fat diet + 0.4g of the Chinese medicine composition of Example 2 / kg body weight gavage Example 3 High-fat diet + 0.4g of the Chinese medicine composition of Example 3 / kg body weight gavage Comparative Example 1 High-fat diet + 0.4g of the Chinese medicine composition of comparative example 1 / kg body weight gavage Comparative Example 2 High-fat diet + 0.4g of the Chinese medicine composition of comparative example 2 / kg body weight gavage Comparative Example 3 High-fat diet + 0.4g of the Chinese medicine composition of comparative example 3 / kg body weight gavage Comparative Example 4 High-fat diet + 0.4g of the Chinese medicine composition of comparative example 4 / kg body weight gavage Comparative Example 5 High-fat diet + 0.4g of the Chinese medicine composition of comparative example 5 / kg body weight gavage The mice were gavaged according to the feeding scheme in Table 1. After 8 weeks of gavage, blood was collected from the tail of each group of mice to measure the levels of cholesterol, triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in serum. The specific results are shown in Table 2.

[0051] Table 2 Serum TC, TG, HDL-C and LDL-C levels of mice in each group Group Cholesterol TC (mmol / L) Triglyceride TG (mmol / L) HDL-C (mmol / L) LDL-C (mmol / L) Blank group 2.05 0.93 0.62 1.01 Model Group 4.36 1.65 0.46 3.15 Example 1 2.96 0.84 0.93 1.65 Example 2 3.07 0.88 0.90 1.77 Example 3 3.12 0.90 0.87 1.84 Comparative Example 1 3.39 0.91 0.83 2.15 Comparative Example 2 3.46 0.94 0.79 2.24 Comparative Example 3 3.34 0.92 0.75 2.17 Comparative Example 4 3.74 1.24 0.61 2.57 Comparative Example 5 3.57 1.18 0.68 2.35 From the data in Table 2, the cholesterol in the blank group is significantly higher than that in the model group, indicating that the mouse hyperlipidemia model was successfully constructed. From the data of the model group and Examples 1-3, it can be seen that taking the tea bags of Examples 1-3 of the present invention can significantly reduce the cholesterol, triglyceride and low-density lipoprotein cholesterol levels in mice, and increase the high-density lipoprotein cholesterol level, indicating that the tea bags of the present invention have excellent lipid-lowering effects. From the data of Example 1 and Comparative Examples 1-2, it can be seen that the enzymatic hydrolysis order of the monarch drug and the type of composite microorganisms affect the lipid-lowering effect; from the data of Example 1 and Comparative Example 3, it can be seen that the change of the auxiliary drug solvent also affects the lipid-lowering effect of the tea bag; from the data of Example 1 and Comparative Examples 4-5, it can be seen that the monarch drug and the auxiliary drug have a significant effect on the lipid-lowering effect of the tea bag.

[0052] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for lowering blood lipids, characterized in that: The invention comprises the following raw materials in parts by weight: 70-80 parts of hawthorn, 50-70 parts of gynostemma pentaphyllum, 35-45 parts of buzia leaves, 20-30 parts of honeysuckle, 3-8 parts of kudingcha, 1-3 parts of pollen cattail and 1-3 parts of liquorice.

2. Use of the Chinese medicine composition according to claim 1 in the preparation of lipid-lowering drugs.

3. A tea bag for lowering blood lipids, characterized in that: The tea bag is made from the Chinese medicine composition according to claim 1.

4. The method for preparing the blood lipid lowering tea bag according to claim 3, characterized in that: include: The method comprises the following steps of: crushing hawthorn, gynostemma pentaphyllum, buzia leaf, honeysuckle, kudingcha and licorice respectively; mixing hawthorn and gynostemma pentaphyllum with water, performing enzymolysis with hemicellulase and nattokinase in turn, fermenting with composite microorganisms, sterilizing and filtering to obtain fermentation liquid; mixing buzia leaf and honeysuckle, performing ultrasonic extraction with ethanol solution, filtering and obtaining filtrate; mixing the fermentation liquid and the filtrate, spray drying and obtaining dry powder; mixing kudingcha, pollen pollen, licorice and the dry powder, sterilizing and putting into tea bags to obtain the lipid-lowering tea bags; the composite microorganisms are composed of purple red yeast rice and schizophyllum in a weight ratio of 1-3:2-6.

5. The preparation method according to claim 4, characterized in that: The weight ratio of the total weight of the hawthorn and Gynostemma pentaphyllum to the weight of water is 1:5-8.

6. The preparation method according to claim 4, characterized in that: The amount of hemicellulase added is 0.5‰-1.3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the hemicellulase enzymolysis is 50-60°C, the pH value is 4-6, and the time is 1-3h; the amount of nattokinase added is 1‰-3‰ of the total weight of hawthorn and gynostemma pentaphyllum, the temperature of the nattokinase enzymolysis is 45-55°C, the pH value is 7-9, and the time is 2-4h.

7. The preparation method according to claim 4, characterized in that: The inoculation amount of the composite microorganism is 4‰-8‰ of the total weight of hawthorn and gynostemma pentaphyllum, the fermentation temperature is 25-35°C, the pH value is 5-6.5, and the fermentation time is 5-10 days.

8. The preparation method according to claim 4, characterized in that: The volume percentage of the ethanol solution is 45%-65%, and the mass ratio of the total mass of the Buza leaves and honeysuckle to the ethanol solution is 1:5-8.

9. The preparation method according to claim 4, characterized in that: The power of the ultrasound is 200-300W.

10. The preparation method according to claim 4, characterized in that: The material-liquid ratio of the extraction is 1:5-8 g / ml, the temperature is 40-50° C., the time is 30-40 min, and the number of extractions is 2-4 times.