Pre-stretching device and preparation method of isotropic pre-stretched silk fibroin film

Abstract

The embodiment of the invention provides a pre-stretching device and a preparation method of an isotropic pre-stretched silk fibroin film.The pre-stretching device comprises a magnetic clamp, a movable shaft rod and a first preset number of base plates, the first preset number of base plates are arranged in parallel, and a preset distance is kept between every two adjacent base plates; a second preset number of tracks in one-to-one correspondence are respectively arranged on the first preset number of substrates; the movable shaft rod is driven by external force to move along the track, and the magnetic clamp drives the to-be-stretched film to move so as to perform isotropic pre-stretching on the to-be-stretched film. By means of the device, radial uniform isotropic quantitative stretching of the to-be-stretched film is achieved, the stretching amplitude can be controlled by adjusting the initial position of the movable shaft rod, and the magnetic clamp is used for fixing the film more conveniently. The pre-stretching device can be used for preparing a standardized and uniform isotropic stretching silk fibroin film, and has the potential of realizing industrial batch production.

Description

Technical Field

[0001] The present application relates to biomedical engineering, and in particular to a pre-stretching device and a method for preparing an isotropic pre-stretched silk fibroin film. Background Art

[0002] Isotropic pre-stretched silk fibroin film is a biomaterial with uniform mechanical properties, which exhibits the same physical and mechanical properties in all directions. Based on its unique super-shrinkage characteristics, the isotropic pre-stretched silk fibroin film has adjustable water-deformation properties. Compared with the swelling behavior of general hydrogel materials in water, this type of protein film exhibits swelling, surface area invariance and shrinkage behaviors depending on the degree of pre-stretching. In particular, for the surface area invariant and shrinkage silk fibroin film, the metal wire structure is prepared on its surface, so that the metal wire structure can maintain structural integrity in a humid environment such as a body fluid environment, thereby ensuring that the device function is stable and can be used for long-term use. However, the current isotropic pre-stretching method cannot achieve uniform stretching of standardized batches, nor can it adapt to the transfer process, which limits the use of the produced silk fibroin film. Therefore, there is an urgent need for a device and preparation method that can be used for the standardized preparation of isotropic pre-stretched silk fibroin film. Summary of the invention

[0003] In order to solve the above technical problems, the present application proposes a pre-stretching device and a method for preparing an isotropic pre-stretched silk fibroin film.

[0004] On the one hand, an embodiment of the present application provides a pre-stretching device, the pre-stretching device comprising a magnetic clamp, a movable shaft and a first preset number of substrates;

[0005] A first preset number of substrates are arranged in parallel, and a preset distance is maintained between adjacent substrates in the first preset number of substrates, and a second preset number of tracks corresponding to each other are respectively arranged on the first preset number of substrates;

[0006] A movable shaft rod is arranged to penetrate a first preset number of substrates, and the movable shaft rod moves along the track direction;

[0007] A magnetic clamp is arranged at one end of the movable shaft rod and is used to fix the film to be stretched; the number of the magnetic clamp, the movable shaft rod and the track is the same;

[0008] The movable shaft rod is driven by an external force and moves along the track, and drives the film to be stretched to move through the magnetic clamp, so as to pre-stretch the film to be stretched.

[0009] Furthermore, the pre-stretching device also includes a limiting device;

[0010] The limiting device is nested on the movable shaft and is arranged between adjacent substrates of a first preset number of substrates.

[0011] Furthermore, a bayonet is provided on the track, and the bayonet is movably connected to the limiting device.

[0012] Further, the limiting device includes a first limiting device, a second limiting device and an elastic driving member;

[0013] The first limiting device and the second limiting device are nested on the movable shaft and are arranged between adjacent substrates of a first preset number of substrates;

[0014] The elastic component is nested on the movable shaft and is arranged between the first limiting device and the second limiting device.

[0015] Further, the magnetic clamp includes a first magnetic clamp and a second magnetic clamp, and the first magnetic clamp and the second magnetic clamp are movably connected;

[0016] The first magnetic clamp and the second magnetic clamp are nested on the movable shaft, and the first magnetic clamp is arranged at one end of the movable shaft, and the second magnetic clamp is arranged on the surface of the first magnetic clamp.

[0017] Furthermore, adjacent substrates among the first preset number of substrates are fixedly connected by screws.

[0018] On the other hand, the present application also provides a method for preparing an isotropic pre-stretched silk fibroin film, which is prepared using the above-mentioned pre-stretching device, characterized in that the method comprises:

[0019] A film to be stretched is provided; the film to be stretched is a silk fibroin film to be stretched;

[0020] By adjusting the position of the movable shaft rod in the track, the starting position of the stretching is determined;

[0021] Fixing the silk fibroin film to be stretched based on a magnetic clamp;

[0022] The movable shaft is driven to move along the track, thereby driving the silk fibroin membrane to be stretched to move, thereby obtaining an isotropic pre-stretched silk fibroin membrane.

[0023] Furthermore, the pre-stretching device also includes a limiting device; the limiting device includes a first limiting device, a second limiting device and an elastic driving member; and a bayonet is also provided on the track;

[0024] After obtaining the film having isotropic mechanical properties, the method further comprises:

[0025] Using an elastic driving member to drive the first limiting device and the second limiting device to move along the movable shaft;

[0026] And an elastic driving member is used to drive the first limiting device and / or the second limiting device into the bayonet, so as to fix the movable shaft rod and avoid movement during the process of transferring the isotropic pre-stretched silk fibroin film.

[0027] Furthermore, the magnetic clamp includes a first magnetic clamp and a second magnetic clamp, and the method of fixing the silk fibroin film to be stretched based on the magnetic clamp includes:

[0028] Placing the silk fibroin film to be stretched on a first magnetic fixture;

[0029] The second magnetic clamp is adsorbed onto the first magnetic clamp, so that the silk fibroin film to be stretched is fixed on the magnetic clamp.

[0030] Furthermore, the magnetic clamp includes a first magnetic clamp and a second magnetic clamp, and the method of fixing the silk fibroin film to be stretched based on the magnetic clamp includes:

[0031] Placing the silk fibroin film to be stretched and the protective film on a first magnetic fixture;

[0032] The second magnetic clamp is adsorbed onto the first magnetic clamp, so that the silk fibroin film to be stretched and the protective film are fixed on the magnetic clamp.

[0033] The embodiment of the present application provides a pre-stretching device and a method for preparing an isotropic pre-stretched silk fibroin film, wherein the pre-stretching device includes a magnetic clamp, a movable shaft and a first preset number of substrates; the first preset number of substrates are arranged in parallel, and the adjacent substrates in the first preset number of substrates maintain a preset distance, and the first preset number of substrates are respectively provided with a one-to-one corresponding track; the movable shaft runs through the first preset number of substrates, and the movable shaft moves along the track direction; the magnetic clamp is arranged at one end of the movable shaft, and is used to fix the film to be stretched; the movable shaft moves along the track under the drive of an external force, and drives the film to be stretched to move through the magnetic clamp, so as to pre-stretch the film to be stretched isotropically. The above device is used to realize the radially uniform isotropic quantitative stretching of the film to be stretched, and the stretching amplitude can be controlled by adjusting the starting position of the movable shaft, and it is more convenient to use a magnetic clamp to fix the film material. The pre-stretching device can be used to prepare standardized, uniformly isotropically stretched silk fibroin films, and has the potential to achieve industrial mass production. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions and advantages in the embodiments of the present application or the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0035] Figure 1 It is a side view of a pre-stretching device provided in an embodiment of the present application before a pre-stretching operation is performed.

[0036] Figure 2 It is a side view of a pre-stretching device provided in an embodiment of the present application before a pre-stretching operation is performed.

[0037] Figure 3 It is a top view of a pre-stretching device provided in an embodiment of the present application before a pre-stretching operation is performed.

[0038] Figure 4 It is a front view of a pre-stretching device provided in an embodiment of the present application before a pre-stretching operation is performed.

[0039] Figure 5 It is a schematic diagram of the process structure of a pre-stretching device performing a pre-stretching operation provided in an embodiment of the present application.

[0040] Figure 6 It is a side view of a pre-stretching device provided in an embodiment of the present application after a pre-stretching operation is performed.

[0041] Figure 7 It is a side view of a pre-stretching device provided in an embodiment of the present application after a pre-stretching operation is performed.

[0042] Figure 8 It is a top view of a pre-stretching device provided in an embodiment of the present application after a pre-stretching operation is performed.

[0043] Fig. 9 It is a front view of a pre-stretching device provided in an embodiment of the present application after a pre-stretching operation is performed.

[0044] Fig.10 It is a schematic diagram of thin film transfer using a transfer bracket provided in an embodiment of the present application.

[0045] Fig.11 The present invention provides a method for preparing an isotropically pre-stretched silk fibroin film.

[0046] Among them, the reference numerals in the figure correspond to:

[0047] 1-base plate; 11-track; 111-bayonet;

[0048] 2-movable shaft; 21-limiting device; 211-first limiting device; 212-second limiting device;

[0049] 3-magnetic clamp; 31-first magnetic clamp; 32-second magnetic clamp;

[0050] 4- screw;

[0051] 51 - bracket body; 521 - first annular clamp; 522 - second annular clamp. DETAILED DESCRIPTION

[0052] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0053] It should be noted that the terms "first", "second", etc. in the specification and claims of the embodiment of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the embodiment of the present application described here can be implemented in an order other than those illustrated or described here. Thus, the features defined as "first" and "second" can expressly or implicitly include one or more of the features. In the description of the present embodiment, unless otherwise specified, the meaning of "multiple" is two or more. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or server comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0054] Isotropic pre-stretched silk fibroin film is a biomaterial with uniform mechanical properties, which exhibits the same physical and mechanical properties in all directions. Based on its unique super-shrinkage characteristics, the isotropic pre-stretched silk fibroin film has adjustable water-deformation properties. Compared with the swelling behavior of general hydrogel materials in water, this type of protein film exhibits swelling, surface area invariance and shrinkage behaviors depending on the degree of pre-stretching. In particular, for the surface area invariant and shrinkage silk fibroin film, the metal wire structure is prepared on its surface, so that the metal wire structure can maintain structural integrity in a humid environment such as a body fluid environment, thereby ensuring that the device function is stable and can be used for long-term use. However, the current isotropic pre-stretching method cannot achieve uniform stretching of standardized batches, nor can it adapt to the transfer process, which limits the use of the produced silk fibroin film. Therefore, there is an urgent need for a device and preparation method that can be used for the standardized preparation of isotropic pre-stretched silk fibroin film.

[0055] Based on this, Figure 1-Figure 5 As shown, an embodiment of the present application provides a pre-stretching device, including a magnetic clamp 3, a movable shaft 2 and a first preset number of substrates 1;

[0056] A first preset number of substrates 1 are arranged in parallel, and a preset distance is maintained between adjacent substrates 1 in the first preset number of substrates 1, and a second preset number of tracks 11 corresponding to each other are respectively arranged on the first preset number of substrates 1;

[0057] A movable shaft 2 is arranged to penetrate a first preset number of substrates 1, and the movable shaft 2 moves along the direction of the track 11;

[0058] A magnetic clamp 3 is provided at one end of the movable shaft 2 and is used to fix the film to be stretched; the number of the magnetic clamp, the movable shaft and the track is the same;

[0059] The movable shaft 2 is driven by an external force to move along the track 11, and drives the film to be stretched to move through the magnetic clamp 3, so as to pre-stretch the film to be stretched.

[0060] Exemplarily, pre-stretching is a material processing technology, which refers to the application of a certain stretching force to the material in advance during the material processing process to cause it to deform to a certain extent. The purpose of this is to enable the material to maintain a certain shape and dimensional stability, or to improve certain properties, during subsequent use or processing. Isotropy refers to the property that the physical, chemical and other properties of an object will not change due to different directions, that is, the performance values ​​of an object measured in different directions are exactly the same, also known as homogeneity. The property that physical properties do not change with the measurement direction. That is, the properties measured along different directions of the object show the same value.

[0061] like Figure 1-Figure 5 As shown, the pre-stretching device includes a first preset number of substrates 1, which are arranged in parallel and correspond to each other up and down; tracks 11 are arranged on the first preset number of substrates 1, and the number and position of the tracks 11 on each substrate 1 are exactly the same; the movable shaft 2 passes through each layer of the substrate 1 through the track 11; the magnetic clamp 3 is nested on the movable shaft 2 and is arranged at the end of the movable shaft 2 away from the substrate 1, that is, it is arranged above the surface of the first preset number of substrates 1, and the function of the magnetic clamp 3 is to fix the film to be stretched.

[0062] The working principle of the above device is: the movable shaft 2 moves along the track 11 under the drive of external force, thereby driving the magnetic clamp 3 and the film to be stretched fixed on the magnetic clamp 3 to move, and the smart man realizes isotropic pre-stretching of the film to be stretched. The structural schematic diagram of the pre-stretching device after the pre-stretching operation is shown in FIG. Figure 5-Figure 9 shown.

[0063] Optionally, to ensure the pre-stretching effect, a second preset number of tracks are evenly arranged on the substrate.

[0064] Optionally, in order to ensure the pre-stretching effect, the shape of the substrate 1 is set to be a circular ring, and the hollow shape in the middle is conducive to the film transfer operation after stretching.

[0065] Optionally, the first preset number of substrates 1 is greater than or equal to two layers of substrates 1 .

[0066] Optionally, there is no limit on the number of tracks 11 on each substrate 1, that is, there is no limit on the second preset number, but to ensure the pre-stretching effect, more tracks 11 should be set as much as possible, that is, as much as possible within the allowable range of substrate space and magnetic clamp size.

[0067] Optionally, the shape of the track 11 is not limited and may be an ellipse, a rectangle, or the like.

[0068] Optionally, the above device can control the stretching degree of pre-stretching by adjusting the starting position or the stretching end position of the movable shaft 2.

[0069] Optionally, the number of magnetic clamps 3 , rails 11 , movable shafts 2 and limiting devices 21 remain consistent.

[0070] Optionally, the length of the movable shaft 2 is greater than the distance between the first substrate 1 and the last substrate 1 among the first preset number of substrates 1 .

[0071] The embodiment of the present application provides a pre-stretching device, including a magnetic clamp 3, a movable shaft 2 and a first preset number of substrates 1, and the first preset number of substrates 1 are provided with tracks 11 corresponding in position and number, so that the movable shaft 2 can move along the track 11 under the drive of an external force, and the film to be stretched can be driven to move by the magnetic clamp 3, so as to perform isotropic pre-stretching on the film to be stretched. The above device is used to achieve radially uniform isotropic quantitative stretching of the film to be stretched, and the stretching amplitude can be controlled by adjusting the starting position of the movable shaft 2. It is more convenient to fix the film material using the magnetic clamp 3, and a large hollow is left in the middle of the device to facilitate the transfer operation of the film after stretching. The device can be used to standardize, uniformly and isotropically stretch the silk fibroin film, and transfer it to other clamps after stretching for further processing, and has the potential to achieve industrial mass production.

[0072] As an optional implementation, the pre-stretching device further includes a limiting device 21 ; the limiting device 21 is nested on the movable shaft 2 and is arranged between adjacent substrates 1 among the first preset number of substrates 1 .

[0073] As an optional embodiment, the limiting device 21 includes a first limiting device 211, a second limiting device 212 and an elastic driving member; the first limiting device 211 and the second limiting device 212 are nested on the movable shaft 2 and are arranged between adjacent substrates 1 among a first preset number of substrates 1; the elastic component is nested on the movable shaft 2 and is arranged between the first limiting device 211 and the second limiting device 212.

[0074] As an optional implementation, a bayonet 111 is further provided on the track 11 , and the bayonet 111 is movably connected to the limiting device 21 .

[0075] For example, Figure 1-Figure 5 As shown, the pre-stretching device also includes a limit device 21, which is nested on the movable shaft 2 and arranged between adjacent substrates 1. The limit device 21 includes a first limit device 211, a second limit device 212 and an elastic component (not shown in the figure). The first limit device 211 and the second limit device 212 are nested on the movable shaft 2 and arranged between adjacent substrates 1 in a first preset number of substrates 1, and the elastic component is arranged between the first limit device 211 and the second limit device 212.

[0076] like Figure 1 and Figure 5 As shown, a bayonet 111 is also provided on the track 11. The bayonet 111 refers to some notches designed on the track 11 board. The top end of the limiting device 21 can be embedded into the bayonet 111 under the drive of the elastic element, so the bayonet 111 and the limiting device 21 are movably connected; specifically, the bayonet 111 is set at a position of the track 11 close to the outer end of the base plate 1, and the limiting device 21 is provided with a structure matching the bayonet 111 for fixing the movable shaft 2.

[0077] like Figure 5 As shown, the first limiting device 211 refers to the limiting device 21 close to the upper substrate 1 among the adjacent substrates 1 , and the second limiting device 212 refers to the limiting device 21 close to the lower substrate 1 among the adjacent substrates 1 .

[0078] The limiting device 21 is used to ensure that the shaft rod does not tilt due to the tension of the film after stretching.

[0079] Through the above structure, after the pre-stretching operation is completed, the elastic element can be used to drive the first limiting device 211 and the second limiting device 212 to fix the movable shaft 2, and the top end of the first limiting device 211 and / or the second limiting device 212 can be embedded in the bayonet 111, thereby preventing the movable shaft 2 from moving and tilting in subsequent operations.

[0080] Optionally, the bayonet 111 can be set on all tracks 11 or part of the track 11 on any substrate 1 of the first preset number of substrates 1, or all tracks 11 or part of the track 11 on all substrates 1. Accordingly, the shape of the limiting device 21 can be adjusted according to the shape of the track 11 and whether the bayonet 111 is set on the track 11.

[0081] Optionally, the shape of the bayonet 111 is not limited and can be any shape.

[0082] Optionally, the elastic component may be a spring, a rubber spring, an elastic alloy, etc.

[0083] The embodiment of the present application uses structures such as the limit device 21 and the bayonet 111 to achieve that after the pre-stretching operation is completed, the limit device 21 and the bayonet 111 are used to ensure that the movable shaft 2 does not tilt or move during the subsequent film transfer process and does not affect the transfer of the film or the performance of the film, thereby effectively ensuring the isotropic performance of the film, that is, effectively ensuring the stretching effect of the pre-stretching device.

[0084] As an optional implementation, the magnetic clamp 3 includes a first magnetic clamp 313 and a second magnetic clamp 323, and the first magnetic clamp 313 and the second magnetic clamp 323 are movably connected;

[0085] The first magnetic clamp 313 and the second magnetic clamp 323 are nested on the movable shaft 2 , and the first magnetic clamp 313 is arranged at one end of the movable shaft 2 , and the second magnetic clamp 32 is arranged on the surface of the first magnetic clamp 31 .

[0086] For example, Figure 5 As shown, the magnetic clamp 3 includes a first magnetic clamp 31 and a second magnetic clamp 32, and the first magnetic clamp 31 and the second magnetic clamp 32 are movably connected and adsorbed by magnetic force. The first magnetic clamp 31 and the second magnetic clamp 32 are both arranged on the movable shaft 2, and the first magnetic clamp 31 is arranged above the uppermost substrate 1 among the first preset number of substrates 1, and the second magnetic clamp 32 is arranged on the surface of the first magnetic clamp 31.

[0087] The combination of the first magnetic clamp 31 and the second magnetic clamp 32 can be used to fix the film to be stretched, that is, the film to be stretched is placed between the two.

[0088] Optionally, the shapes of the first magnetic clamp 31 and the second magnetic clamp 32 are not limited and may be cylindrical, square, or the like.

[0089] The embodiment of the present application fixes the film to be stretched by the first magnetic clamp 31 and the second magnetic clamp 32, so that the film fixing function in the pre-stretching device is simpler and more convenient, and saves space, and the fixing method is stable, which is conducive to the transfer process after the pre-stretching operation is completed.

[0090] As an optional implementation manner, adjacent substrates 1 in the first preset number of substrates 1 are fixedly connected via screws 4 .

[0091] For example, Figure 1 As shown, adjacent substrates 1 among the first preset number of substrates 1 are fixedly connected via screws 4 , and the length of the screws 4 is equal to the preset distance between the adjacent substrates 1 .

[0092] Optionally, the number of screw rods 4 is greater than or equal to 2.

[0093] In the embodiment of the present application, the first preset number of screws is fixed by the screw rod 4, thereby improving the stability of the pre-tensioning device.

[0094] As an optional embodiment, the pre-stretching device also includes a separately arranged transfer bracket, the transfer bracket includes a bracket body 51 and an annular clamp, the annular clamp includes a first annular clamp 521 and a second annular clamp 522, the first annular clamp 521 is connected to the bracket body 51, and the second annular clamp 522 is movably connected to the first annular clamp 521.

[0095] For example, Fig.10 As shown, the above-mentioned transfer bracket is used to transfer the film prepared by the pre-stretching device, and the specific method of use is as follows:

[0096] The second annular clamps 522 in the two transfer brackets are removed, and two transfer brackets without the second annular clamps 522 are placed in parallel in the hollow area in the middle of the pre-stretching device, and the prepared film is placed on the first annular clamps 521 of the two transfer brackets, and the second annular clamps 522 are pressed down to connect with the first annular clamps 521 by magnetic force; and the prepared film is cut along the edge of the second annular clamp 522, that is, the prepared film is transferred to the transfer bracket, and then the pre-stretched film can be transferred to other clamps for further processing.

[0097] Optionally, the first annular clamp 521 and the second annular clamp 522 may be connected magnetically.

[0098] It should be noted that the transfer bracket may also be in other forms, as long as it can achieve the complete transfer of the pre-stretched film.

[0099] On the other hand, the present application also provides a method for preparing an isotropic pre-stretched silk fibroin film, which is prepared using the above-mentioned pre-stretching device, and the preparation method comprises:

[0100] S10: providing a film to be stretched; the film to be stretched is a silk fibroin film to be stretched;

[0101] S20: Determine the starting position of stretching by adjusting the position of the movable shaft rod 2 in the track 11;

[0102] S30: Fixing the silk fibroin film to be stretched based on the magnetic clamp 3;

[0103] S40: driving the movable shaft 2 to move along the track 11, thereby driving the silk fibroin membrane to be stretched to move, thereby obtaining an isotropic pre-stretched silk fibroin membrane.

[0104] For example, Fig.11 As shown, the process of preparing an isotropic pre-stretched silk fibroin film using the pre-stretching device provided in the embodiment of the present application is as described in S10-S40.

[0105] Specifically, in step S20, the starting position of stretching can be determined by adjusting the position of the movable shaft rod 2 in the track 11, and then the final stretching amplitude can be determined, because the stretching amplitude is related to the stretching distance.

[0106] In step S40, in order to ensure the effect of pre-stretching, when driving the movable shaft rods 2 to move along the track 11, it is necessary to ensure that all movable shaft rods 2 move simultaneously, and the moving speed and moving position are consistent. Figure 5-Figure 9 As shown, the movable shaft rod 2 is pushed to the outermost side of the track 11 , that is, the side close to the outer circle of the base plate 1 .

[0107] It should be noted that the silk fibroin membrane to be stretched described in the present application is a silk fibroin membrane prepared based on the prior art. The present application does not limit the preparation process of the silk fibroin membrane to be stretched.

[0108] The embodiment of the present application utilizes a pre-stretching device to perform a pre-stretching operation on the silk fibroin membrane to be stretched, thereby preparing an isotropic pre-stretched silk fibroin membrane. The isotropic pre-stretched silk fibroin membrane prepared by this method has the advantages of controllable and uniform stretching amplitude, adjustable stretching amplitude, and easy transfer. The prepared isotropic pre-stretched silk fibroin membrane has adjustable water-induced deformation properties and can be widely used in the preparation of various bioelectronic devices. The preparation of isotropic pre-stretched silk fibroin membrane based on this method has the potential for industrial mass production.

[0109] As an optional embodiment, the pre-stretching device further includes a limiting device 21; the limiting device 21 includes a first limiting device 211, a second limiting device 212 and an elastic driving member; a bayonet 111 is further provided on the track 11;

[0110] After the above method S40, that is, after the film with isotropic mechanical properties is obtained, the above method further includes:

[0111] Using an elastic driving member, driving the first limiting device 211 and the second limiting device 212 to move along the movable shaft 2;

[0112] And an elastic driving member is used to drive the first limiting device 211 and / or the second limiting device 212 into the bayonet 111 to fix the movable shaft 2 to avoid movement during the transfer of the isotropic pre-stretched silk fibroin film.

[0113] For example, Figure 5 As shown, after the isotropic pre-stretched silk fibroin film is prepared, in order to facilitate its transfer, an elastic driving member is used to drive the first limiting device 211 and the second limiting device 212 to move up and down along the movable shaft 2, which can prevent the movable shaft 2 from tilting. Continuing to use the elastic driving member to drive the first limiting device 211 and the second limiting device 212 to move up and down along the movable shaft 2 can make the first limiting device 211 and / or the second limiting device 212 enter the bayonet 111, thereby preventing the movable shaft 2 from moving and preventing it from moving during the process of transferring the isotropic pre-stretched silk fibroin film.

[0114] Optionally, the present application embodiment also provides a method for transferring an isotropic pre-stretched silk fibroin film, which is based on a transposition support to transfer the isotropic pre-stretched silk fibroin film prepared by a pre-stretching device, such as Fig. 9 As shown, the specific usage is:

[0115] The second annular clamps 522 in the two transfer brackets are removed, and two transfer brackets without the second annular clamps 522 are placed in parallel in the hollow area in the middle of the pre-stretching device, and the isotropic pre-stretched silk fibroin film is placed on the first annular clamps 521 of the two transfer brackets, and the second annular clamps 522 are pressed down to connect with the first annular clamps 521 through magnetic force; and the prepared film is cut along the edge of the second annular clamp 522, that is, the prepared film is transferred to the transfer bracket, and then the isotropic pre-stretched silk fibroin film can be transferred to other clamps for further processing.

[0116] After the pre-stretching operation is completed, the embodiment of the present application uses an elastic driving member to axially drive the first limit device 211 and the second limit device 212, and then matches and connects with the bayonet 111, thereby ensuring that the movable shaft 2 will not tilt or move during the subsequent transfer of the isotropic pre-stretched silk fibroin film, thereby affecting the transfer of the isotropic pre-stretched silk fibroin film or affecting the performance of the isotropic pre-stretched silk fibroin film, thereby effectively ensuring the isotropic performance of the isotropic pre-stretched silk fibroin film, that is, effectively ensuring the stretching effect of the pre-stretching device.

[0117] As an optional embodiment, the magnetic clamp 3 includes a first magnetic clamp 31 and a second magnetic clamp 32. In the above step S30, the above method of fixing the silk fibroin film to be stretched based on the magnetic clamp 3 includes:

[0118] S301: placing the silk fibroin film to be stretched on the first magnetic fixture 31;

[0119] S302 : adsorbing the second magnetic clamp 32 onto the first magnetic clamp 31 , so that the silk fibroin film to be stretched is fixed on the magnetic clamp 3 .

[0120] As an optional embodiment, the magnetic clamp 3 includes a first magnetic clamp 31 and a second magnetic clamp 32. In the above step S30, the above method of fixing the silk fibroin film to be stretched based on the magnetic clamp 3 includes:

[0121] S3001: placing the silk fibroin film to be stretched and the protective film on a first magnetic fixture 31;

[0122] S3002 : adsorbing the second magnetic clamp 32 onto the first magnetic clamp 31 , so that the silk fibroin film to be stretched and the protective film are fixed on the magnetic clamp 3 .

[0123] Exemplarily, the processes S301-S302 and S3001-S3002 are both about how to use the magnetic clamp 3 to fix the silk fibroin film to be stretched, and the difference lies in whether a protective film is included, that is, before the silk fibroin film is fixed by the magnetic clamp 3, the protective film is first placed on the first magnetic clamp 31. This can not only reduce the direct wear of the film during the stretching process, but also prevent pollutants or chemicals on the stretching apparatus from causing potential damage to the silk fibroin film, thereby ensuring the integrity and stretching quality of the silk fibroin film. In addition, the use of the protective film can also reduce electrostatic adsorption during the stretching process to a certain extent, and improve the safety and efficiency of the operation.

[0124] In the above process, if a protective film is included, the protective film is first placed on each first magnetic clamp 31, and then the silk fibroin film to be stretched is placed on the protective film; if no protective film is included, the silk fibroin film to be stretched is directly placed on each first magnetic clamp 31.

[0125] Then, the second magnetic clamp 32 is adsorbed to the first magnetic clamp 31 by magnetic force, so that the silk fibroin film to be stretched and the protective film are fixed to the magnetic clamp 3 .

[0126] It should be noted that whether a protective film is needed and the specific material of the protective film are determined according to the final use of the pre-stretched isotropic silk fibroin film.

[0127] The embodiment of the present application fixes the silk fibroin film to be stretched by the first magnetic clamp and the second magnetic clamp, so that the fixing method of the silk fibroin film to be stretched is simpler, more convenient and more stable, which is beneficial to the transfer process after the pre-stretching operation is completed.

[0128] The present application also provides an application example of the isotropic pre-stretched silk fibroin film prepared by the above method: a long-term and reliable brain electrode based on the isotropic pre-stretched silk fibroin film.

[0129] Specifically, the long-term reliable brain electrode is based on the method described in the embodiment of the present application to prepare an isotropic pre-stretched silk protein film, and a patterned metal conductive structure is prepared on the surface of the protein film by electron beam metal evaporation. Due to the super-contraction characteristics brought by pre-stretching, the protein film will not deform along the surface of the film when it comes into contact with water, which can ensure the stability of the metal conductive structure in a humid environment. At the same time, since silk protein has good biocompatibility, the electrode can be used to record EEG signals in vivo for a long time.

[0130] It should be noted that the above-mentioned sequence of the embodiments of the present application is for description only and does not represent the advantages and disadvantages of the embodiments. The above-mentioned specific embodiments of this specification are described. Other embodiments are within the scope of the attached claims. In some cases, the actions or steps recorded in the claims can be performed in an order different from that in the embodiments and still achieve the desired results. In addition, the processes depicted in the drawings do not necessarily require the specific order or continuous order shown to achieve the desired results. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.

[0131] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the device and server embodiments, since they are basically similar to the method embodiments, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiments.

[0132] A person skilled in the art will understand that all or part of the steps to implement the above embodiments may be accomplished by hardware, or by a program to instruct the relevant hardware, and the program may be stored in a computer-readable storage medium, and the above-mentioned storage medium may be a read-only memory, a disk or an optical disk, etc.

[0133] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A pre-stretching device, characterized in that: The pre-stretching device comprises a magnetic clamp (3), a movable shaft (2) and a first preset number of substrates (1); The first preset number of substrates (1) are arranged in parallel, and a preset distance is maintained between adjacent substrates (1) in the first preset number of substrates (1), and a second preset number of tracks (11) in one-to-one correspondence are respectively arranged on the first preset number of substrates (1); The movable shaft (2) is arranged to penetrate the first preset number of substrates (1), and the movable shaft (2) moves along the direction of the track (11); The magnetic clamp (3) is arranged at one end of the movable shaft (2) and is used to fix the film to be stretched; the number of the magnetic clamp, the movable shaft and the rail is the same; The movable shaft (2) is driven by an external force to move along the track (11), and drives the film to be stretched to move through the magnetic clamp (3), so as to perform pre-stretching on the film to be stretched.

2. The pre-stretching device according to claim 1, characterized in that: The pre-stretching device also includes a limiting device (21); The limiting device (21) is nested on the movable shaft (2) and is arranged between adjacent substrates (1) among the first preset number of substrates (1).

3. The pre-stretching device according to claim 2, characterized in that: The track (11) is also provided with a bayonet (111), and the bayonet (111) is movably connected to the limiting device (21).

4. The pre-stretching device according to claim 2, characterized in that: The limiting device (21) comprises a first limiting device (211), a second limiting device (212) and an elastic driving member; The first limiting device (211) and the second limiting device (212) are nested on the movable shaft (2) and are arranged between adjacent substrates (1) among the first preset number of substrates (1); The elastic component is nested on the movable shaft (2) and is arranged between the first limiting device (211) and the second limiting device (212).

5. The pre-stretching device according to claim 1, characterized in that: The magnetic clamp (3) comprises a first magnetic clamp (31) and a second magnetic clamp (32), wherein the first magnetic clamp (31) (3) and the second magnetic clamp (32) are movably connected; The first magnetic clamp (31) (3) and the second magnetic clamp (32) are nested on the movable shaft (2), and the first magnetic clamp (31) is arranged at one end of the movable shaft (2), and the second magnetic clamp (32) is arranged on the surface of the first magnetic clamp (31).

6. The pre-stretching device according to claim 1, characterized in that: Adjacent substrates (1) among the first preset number of substrates (1) are fixedly connected via screw rods (4).

7. A method for preparing an isotropic pre-stretched silk fibroin film, using the pre-stretching device according to any one of claims 1 to 6, characterized in that: The method comprises: A film to be stretched is provided; the film to be stretched is a silk fibroin film to be stretched; The starting position of stretching is determined by adjusting the position of the movable shaft rod (2) in the track (11); Fixing the silk fibroin film to be stretched based on a magnetic clamp (3); The movable shaft (2) is driven to move along the track (11), thereby driving the silk fibroin membrane to be stretched to move, thereby obtaining an isotropic pre-stretched silk fibroin membrane.

8. The preparation method according to claim 7, characterized in that: The pre-stretching device further comprises a limiting device (21); the limiting device (21) comprises a first limiting device (211), a second limiting device (212) and an elastic driving member; a bayonet (111) is further provided on the track (11); After obtaining the film with isotropic mechanical properties, the method further comprises: Using an elastic driving member to drive the first limiting device (211) and the second limiting device (212) to move along the movable shaft (2); And using an elastic driving member, driving the first limiting device (211) and / or the second limiting device (212) into the bayonet (111) to fix the movable shaft (2) to avoid movement during the transfer of the isotropic pre-stretched silk fibroin film.

9. The preparation method according to claim 7, characterized in that: The magnetic clamp (3) comprises a first magnetic clamp (31) and a second magnetic clamp (32), and the method of fixing the silk fibroin film to be stretched based on the magnetic clamp (3) comprises: Placing the silk fibroin film to be stretched on the first magnetic fixture (31); The second magnetic clamp (32) is adsorbed onto the first magnetic clamp (31) so that the silk fibroin film to be stretched is fixed on the magnetic clamp (3).

10. The preparation method according to claim 7, characterized in that: The magnetic clamp (3) comprises a first magnetic clamp (31) and a second magnetic clamp (32), and the method of fixing the silk fibroin film to be stretched based on the magnetic clamp (3) comprises: Placing the silk fibroin film to be stretched and the protective film on the first magnetic fixture (31) (3); The second magnetic clamp (32) is adsorbed onto the first magnetic clamp (31), so that the silk fibroin film to be stretched and the protective film are fixed to the magnetic clamp (3).

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