Efficient electrogenesis microbial fuel cell as well as preparation and application thereof

Abstract

The invention relates to the technical field of microbial fuel biological cells, in particular to an efficient electrogenesis microbial fuel cell as well as preparation and application thereof. The method comprises the following steps: firstly, carrying out anaerobic domestication on current-producing bacteria in active sludge subjected to sieving and impurity removal to obtain mixed current-producing bacteria; then putting the mixed current-producing bacteria into an anaerobic basic culture medium containing phenazine-1-carboxylic acid for enrichment culture to obtain enriched mixed current-producing bacteria; and finally, putting the enriched mixed electrogenesis bacteria and the anolyte into an anode chamber of a microbial fuel cell double-chamber reactor, and then adding the catholyte into a cathode chamber to obtain the microbial fuel cell capable of efficiently generating electricity. Wherein in the anaerobic domestication process, the salt tolerance of the current-producing bacteria in the activated sludge is enhanced by utilizing the stress pressure of jump salinity. According to the method, the electricity-producing bacteria are cultured by adopting a jumping type salinity gradient stimulation method, and the obtained MFC is suitable for application scenes of chemical high-salinity wastewater, so that the method has a relatively good industrialization prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fuel bio-batteries, and in particular to a high-efficiency electricity-generating microbial fuel cell and its preparation and application. Background Art

[0002] Energy has always affected people's quality of life. With the rapid development of urbanization and industrialization, the energy crisis has continued to intensify. A series of environmental pollution problems have arisen in the process of energy use. The high discharge of high-salinity wastewater has also brought tremendous pressure to water environment protection. High-salinity wastewater is wastewater with a total salt content of more than 1%. Its composition is complex and presents complex pollution characteristics, that is, the COD value in the water body is high, the biodegradability is poor, and the toxicity is strong. Therefore, the development of new, environmentally friendly, renewable energy and energy supply methods is the main idea of ​​future energy development. Among all kinds of clean energy, microbial fuel cells are a very promising technology. As a new energy supply method, microbial fuel cells can convert chemical energy in wastewater into electrical energy. They have the advantages of cleanliness, mild operating conditions, and good environmental compatibility, and have always received widespread attention.

[0003] Microbial fuel cells (MFCs) use the catalytic activity of microorganisms to decompose organic matter and release protons and electrons. Protons are transferred from the anode to the cathode for reduction reaction, and electrons move to the cathode through the external circuit to form an electric current. Electroactive microorganisms play a very important role in biodegradation and power generation. The activity of microorganisms directly affects the rate and total amount of electron transfer. Exoelectric bacteria and electrotrophic bacteria are two major types of conductive microorganisms. Exoelectric bacteria transfer electrons to the electrode surface, while electrotrophic bacteria obtain electrons from the electrode. They are widely used as biocatalysts in different types of systems. The exchange of electrons between the outer surface and the inside of the cell has a crucial influence on the overall performance in bioelectrochemical systems, especially in microbial fuel cells. This electron exchange process not only determines the energy conversion efficiency, but also directly regulates the metabolic function of the cell. Compared with single bacteria, electroactive microbial communities composed of multiple species establish a bacterial substance energy metabolism network through cell metabolic cascade reactions and aerobic and anaerobic respiratory chains, which can effectively broaden the spectrum of available substrates and improve electron transfer to enhance environmental stability. During the startup phase of a microbial fuel cell, the influence of the components of the anolyte on the microorganisms, as well as the changes in the number and type of electrogenic bacteria after the inoculated bacteria enter the battery, are highly random. These problems have a significant impact on the battery startup time, energy consumption, the activity, number and type diversity of anaerobic electrogenic bacteria, and the ultimate efficiency and power generation capacity of the microbial fuel cell. Therefore, seeking a method for domesticating anolyte electrogenic bacteria with high power generation efficiency and stable performance is a feasible strategy to increase the power generation of microbial fuel cells and promote the development of MFC technology.

[0004] In the prior art, Chinese patent CN 116093390 A discloses the application of supernatant of Pseudomonas aeruginosa in microbial fuel cells. This method mainly adds supernatant of Pseudomonas aeruginosa containing a large amount of phenazine to the anode chamber of the microbial fuel cell, killing most of the miscellaneous bacteria that do not have the ability to transfer extracellular electrons, ensuring that the abundance of electrogenic bacteria on the microbial membrane is maintained at a high level. At the same time, phenazine can also serve as an electron transfer mediator to avoid the internal consumption of electrons generated during the oxidation of organic matter, so as to increase the power generation capacity of MFC. This method has a certain effect in increasing the abundance of electrogenic bacteria in the anode chamber, but it requires a certain amount of time to promote the growth of electrogenic bacteria, thereby affecting the startup speed of MFC.

[0005] Patent CN 116111156 A discloses a stable and efficient electricity-generating microbial fuel cell and its preparation method and application. Four different types of electricity-generating mixed bacteria are selected and mixed in equal volume ratios as anode inoculation sources, and added to the anode chamber at a volume ratio of 1:1 to 5 with the anode liquid. The cathode liquid is a potassium ferrocyanide solution to extend the duration of stable voltage. This method has a certain effect on improving the electricity generation of MFC, but it does not reduce the consumption and interference of most of the miscellaneous bacteria that do not have the ability to transfer extracellular electrons on the organic matter in the anode chamber.

[0006] Patent CN 112795528 A discloses a method for taming an electrogenic microbial community in a microbial fuel cell. By supplying voltage to the microbial anode and cathode components through an external power supply, the ability of the electrogenic microbial community in the anode of the microbial fuel cell to generate and transfer electrons is significantly improved, the growth of the anode biofilm is promoted, the relative abundance of the electrogenic microbial community is increased, and the power generation performance of the microbial fuel cell is thereby improved. This method has certain feasibility, but the enrichment of electrogenic bacteria requires an external power supply, which increases additional consumption and has the disadvantage of energy consumption.

[0007] Therefore, it is very important to provide a technical solution that can solve the above technical problems. Summary of the invention

[0008] In order to solve the above problems, the purpose of the present invention is to provide a highly efficient electricity-generating microbial fuel cell and its preparation and application. The present invention strengthens the salt tolerance of the electrogenic bacteria by applying a jump-type salinity stress pressure, and at the same time, adds phenazine-1-carboxylic acid (PCA) with a bacteria-inhibiting effect to the culture medium to improve the purity of the electrogenic bacteria, thereby enriching and culturing salt-tolerant and highly efficient electrogenic bacteria, and further obtaining a highly efficient electricity-generating microbial fuel cell.

[0009] The purpose of the present invention can be achieved by the following technical solutions:

[0010] The first object of the present invention is to provide a method for preparing a highly efficient electricity-generating microbial fuel cell, comprising the following steps:

[0011] (S1) performing anaerobically acclimatization on the electrogenic bacteria in the activated sludge after screening and impurity removal to obtain mixed electrogenic bacteria;

[0012] (S2) placing the mixed electrogenic bacteria prepared in step (S1) in an anaerobic basal culture medium containing phenazine-1-carboxylic acid for enrichment culture to obtain enriched mixed electrogenic bacteria;

[0013] (S3) placing the enriched mixed electrogenic bacteria and the anolyte prepared in step (S2) in the anode chamber of a microbial fuel cell dual-chamber reactor, and then adding the cathode liquid into the cathode chamber to obtain a microbial fuel cell with high efficiency in generating electricity;

[0014] Among them, in step (S1), during the anaerobic acclimation process, the salt tolerance of the electrogenic bacteria in the activated sludge is enhanced by using the stress pressure of jump salinity.

[0015] In one embodiment of the present invention, in step (S1), the anaerobic acclimation process is specifically as follows:

[0016] (101) Using the activated sludge after sieving and removing impurities as an inoculant, mixing it with a carbon source and an anolyte, placing it in the anode chamber of the first microbial fuel cell, and stably operating it for one cycle;

[0017] (102) using the reaction solution in the anode chamber of the first microbial fuel cell as an inoculant, mixing it with a carbon source and an anode solution containing 18 to 22 g / L of sodium chloride, placing it in the anode chamber of the second microbial fuel cell, and stably operating it for one cycle;

[0018] (103) using the reaction solution in the anode chamber of the second microbial fuel cell as an inoculant, mixing it with a carbon source and an anode solution containing 8 to 12 g / L of sodium chloride, and placing it in the anode chamber of the third microbial fuel cell, and stably operating it for one cycle;

[0019] (104) using the reaction liquid in the anode chamber of the third microbial fuel cell as an inoculant, mixing it with a carbon source and an anode liquid containing 13 to 17 g / L of sodium chloride, placing it in the anode chamber of the fourth microbial fuel cell, and operating it stably for one cycle;

[0020] (105) using the reaction liquid in the anode chamber of the fourth microbial fuel cell as an inoculant, mixing it with a carbon source and an anode liquid containing 3 to 7 g / L of sodium chloride, and placing it in the anode chamber of the fifth microbial fuel cell, stably operating for one cycle to complete the anaerobic acclimation of the electrogenic bacteria in the activated sludge;

[0021] The cathode liquid in the cathode chamber of the first microbial fuel cell, the second microbial fuel cell, the third microbial fuel cell, the fourth microbial fuel cell and the fifth microbial fuel cell is a 0.1-0.5 mol / L potassium ferrocyanide solution.

[0022] In one embodiment of the present invention, the activated sludge is sieved using a 200-mesh screen to remove impurities in the activated sludge.

[0023] In one embodiment of the present invention, in a microbial fuel cell dual-chamber reactor, the anode chamber and the cathode chamber are separated by a proton exchange membrane, the cathode and the anode are connected by a titanium wire (resistance of 1000Ω), and finally the titanium wire is connected to an external resistor to form a closed loop.

[0024] In one embodiment of the present invention, the anode chamber is subjected to nitrogen blowing and deoxygenation treatment before operation, and is sealed to maintain an anaerobic state to avoid interference with the attachment and growth of anode microorganisms;

[0025] During operation, the temperature is 15-40°C.

[0026] In one embodiment of the present invention, the volume ratio of the inoculant to the anolyte is 1:2 to 1:4;

[0027] Preferably, the volume ratio of the inoculant amount to the anolyte is 1:3.

[0028] In one embodiment of the present invention, in step (S2), the anode containing mixed electrogenic bacteria in the anode chamber of the fifth microbial fuel cell is used as an enrichment substrate for enrichment culture.

[0029] In one embodiment of the present invention, in step (S2), the concentration of phenazine-1-carboxylic acid in the anaerobic basal medium containing phenazine-1-carboxylic acid is 10 to 30 mg / L;

[0030] The anaerobic basal medium included the following components at the following concentrations:

[0031] 10-16 g / L peptone, 5-10 g / L yeast extract, 4-10 g / L sodium chloride, 1 g / L starch, 1-3 g / L glucose, 1 g / L sodium pyruvate, 1 g / L arginine, 0.5 g / L sodium succinate, 0.4 g / L sodium bicarbonate, 0.5-1 g / L L-cysteine ​​hydrochloride, 0.5 g / L ferric pyrophosphate, 0.005 g / L hemoglobin, 0.0005-0.001 g / L vitamin K, 1 g / L DTT, 0.5 g / L sodium thioglycolate, and the solvent is deionized water.

[0032] In one embodiment of the present invention, phenazine-1-carboxylic acid mother liquor is prepared by the following method: 0.02 g of phenazine-1-carboxylic acid is weighed, 4.0 mL of dimethyl sulfoxide is used to fully dissolve phenazine-1-carboxylic acid, and the final concentration of phenazine-1-carboxylic acid mother liquor is 5 mg / mL.

[0033] In one embodiment of the present invention, the concentration of phenazine-1-carboxylic acid in the anaerobic basal medium containing phenazine-1-carboxylic acid is 15 mg / L.

[0034] In one embodiment of the present invention, in step (S2), during the enrichment culture, the temperature is 15-40°C and the rotation speed is 50-300 rpm until the OD of the bacterial suspension reaches 600 It is 0.6~1.

[0035] In one embodiment of the present invention, after the enrichment culture is completed, the enrichment culture solution is centrifuged at 6000 rpm for 5 to 10 minutes, and the precipitate is the enriched mixed electrogenic bacteria.

[0036] In one embodiment of the present invention, the anolyte comprises the following components in concentrations:

[0037] 1.42g / L sodium acetate, 1-4g / L glucose, 6.57g / L potassium dihydrogen phosphate, 2.88g / L potassium dihydrogen phosphate, 0.2-0.4g / L ammonium chloride, 0.13g / L potassium chloride, 5-10mL / L vitamin solution, 10-15mL / L trace element solution, the solvent is deionized water;

[0038] The cathode liquid is a 0.1-0.5 mol / L potassium ferrocyanide solution.

[0039] In one embodiment of the present invention, the vitamin solution comprises the following components in the following concentrations:

[0040] 5mg / L vitamin B1, 5mg / L vitamin B2, 5mg / L vitamin B3, 5mg / L vitamin B5, 10mg / L vitamin B6, 2mg / L vitamin B7, 2mg / L vitamin B9, 1mg / L vitamin B12, 5mg / L p-aminobenzoic acid, 5mg / L lipoic acid, the solvent is deionized water;

[0041] The trace element solution includes the following components in concentration:

[0042] 1.5g / L aminotriacetic acid, 1g / L sodium chloride, 3g / L MgSO4·7H2O, 0.5g / L MnSO4·H2O, 0.1g / L FeSO4·7H2O, 0.152g / L CoCl2·6H2O, 0.1g / L CaCl2·2H2O, 0.1g / L ZnSO4, 0.1g / L CuSO4·5H2O, 0.02g / L KAl(SO4)2·12H2O, 0.01g / L boric acid, 0.01g / LNa2MoO4·2H2O, 0.03g / L NiSO4·6H2O, 1mg / L Na2SeO3·5H2O, the solvent is deionized water.

[0043] In one embodiment of the present invention, the anode chamber and cathode chamber in the dual-chamber reactor of the microbial fuel cell are separated by a pretreated Nafion 117 proton exchange membrane;

[0044] Specifically, the preprocessing method is as follows:

[0045] The Nafion 117 proton exchange membrane was boiled in hydrogen peroxide and then immersed in a 5% sodium chloride solution.

[0046] In one embodiment of the present invention, the anode and the cathode are both modified carbon felt obtained by modification pretreatment, which specifically includes the following steps:

[0047] The carbon felt was soaked in 0.1mol / L hydrochloric acid solution for 12 to 36 hours, rinsed with deionized water until neutral, then soaked in 1mol / L sodium hydroxide solution for 12 to 24 hours, rinsed with deionized water until neutral, and dried at 105°C for 3 hours; then soaked in an acidic potassium dichromate solution composed of 1mol / L sulfuric acid and 0.4mol / L potassium dichromate, heated in a 60°C water bath for 1 hour, repeatedly rinsed with deionized water until neutral, and then dried, then placed in a muffle furnace, and heated at 450°C for 30 minutes to obtain a modified carbon felt.

[0048] The second object of the present invention is to provide a high-efficiency electricity-generating microbial fuel cell prepared by the above method.

[0049] In one embodiment of the present invention, when the output voltage at both ends of the high-efficiency electricity-generating microbial fuel cell is monitored to be stable, it indicates that the high-efficiency electricity-generating microbial fuel cell is successfully started.

[0050] The third object of the present invention is to provide an application of a highly efficient electricity-generating microbial fuel cell in the field of high-salinity wastewater treatment.

[0051] Compared with the prior art, the present invention has the following beneficial effects:

[0052] (1) The present invention adopts a jumping salinity gradient stimulation method to cultivate electrogenic bacteria. The obtained MFC is suitable for the application scenario of chemical high-salt wastewater. This method is effective for any type of activated sludge and therefore has a good industrial prospect.

[0053] (2) The present invention applies the phenazine-1-carboxylic acid (PCA) bacteria inhibition method to the enrichment culture of electrogenic bacteria. The reduction of bacteria and the strengthening of the electrogenic bacteria group greatly enhance the power generation capacity of the obtained MFC device, significantly shorten the startup time, and have good industrial prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 The voltage variation diagram of MFC-1 and MFC-2;

[0055] Figure 2 The voltage variation diagrams of MFC-3, MFC-4 and MFC-7;

[0056] Figure 3 The voltage variation diagrams of MFC-5, MFC-6 and MFC-8;

[0057] Figure 4 This is the power density curve of MFC-1, MFC-3 and MFC-7.

[0058] Figure 5 This is the power density curve of MFC-2, MFC-4 and MFC-8. DETAILED DESCRIPTION

[0059] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0060] In the following examples, the activated sludge is derived from the secondary sedimentation tank of a chemical and pharmaceutical company's wastewater treatment.

[0061] The anolyte solution includes the following components in the following concentrations:

[0062] 1.42g / L sodium acetate, 1g / L glucose, 6.57g / L potassium dihydrogen phosphate, 2.88g / L potassium dihydrogen phosphate, 0.31g / L ammonium chloride, 0.13g / L potassium chloride, 6mL / L vitamin solution, 15mL / L trace element solution, the solvent is deionized water;

[0063] The mother solution of phenazine-1-carboxylic acid is a 5 mg / mL solution of phenazine-1-carboxylic acid (the solvent is dimethyl sulfoxide);

[0064] The anaerobic basal medium included the following components at the following concentrations:

[0065] 16g / L peptone, 6g / L yeast extract, 5g / L sodium chloride, 1g / L starch, 1g / L glucose, 1g / L sodium pyruvate, 1g / L arginine, 0.5g / L sodium succinate, 0.4g / L sodium bicarbonate, 0.5g / L L-cysteine ​​HCl, 0.5g / L ferric pyrophosphate, 0.005g / L hemoglobin, 0.0005g / L vitamin K, 1g / L DTT, 0.5g / L sodium thioglycolate, the solvent is deionized water; sterilize at 121℃, 105KPa for 30min.

[0066] 60% glycerol was prepared by the following method: 60 mL of glycerol was added to a 100 mL volumetric flask, the volume was fixed with ultrapure water, sterilized at 121° C. and 105 kPa for 30 min, and stored at room temperature.

[0067] Method for preserving bacterial strains: add 0.75 mL of bacterial solution to 0.25 mL of 60% glycerol and mix thoroughly to obtain a mixed solution; place the mixture at room temperature for 30 minutes and then transfer it to an ultra-low temperature refrigerator for freezing.

[0068] The first microbial fuel cell, the second microbial fuel cell, the third microbial fuel cell, the fourth microbial fuel cell and the fifth microbial fuel cell used below are all microbial fuel cell dual-chamber reactors; wherein the working volumes of the cathode chamber and the anode chamber are both 120 mL, the anode chamber and the cathode chamber are separated by a proton exchange membrane (pretreated Nafion 117 proton exchange membrane: the Nafion 117 proton exchange membrane is placed in hydrogen peroxide and boiled, and then immersed in a 5wt% sodium chloride solution), an anode electrode is arranged in the anode chamber, and a cathode electrode is arranged in the cathode chamber; a titanium wire with a diameter of 0.6 mm is used to connect the cathode and cathode circuits, and a 1000Ω resistor is used to connect the external circuit to form a closed loop.

[0069] The anode electrode and cathode electrode are both 2 mm thick and 9 cm in area. 2 (3cm*3cm) modified carbon felt;

[0070] The modified carbon felt is prepared by the following method:

[0071] The carbon felt was soaked in 0.1mol / L hydrochloric acid solution for 24h, rinsed with deionized water until neutral, then soaked in 1mol / L sodium hydroxide solution for 18h, rinsed with deionized water until neutral, and dried at 105℃ for 3h; then soaked in an acidic potassium dichromate solution composed of 1mol / L sulfuric acid and 0.4mol / L potassium dichromate, heated in a 60℃ water bath for 1h, repeatedly rinsed with deionized water until neutral, and then dried, then placed in a muffle furnace, and heated at 450℃ for 30min to obtain a modified carbon felt.

[0072] Before the operation of the dual-chamber reactor of the microbial fuel cell (after the inoculant and the anode liquid are added), the anode chamber is deoxygenated by nitrogen blowing and sealed to maintain an anaerobic state to avoid interference with the attachment and growth of anode microorganisms; the potassium ferrocyanide solution in the cathode chamber needs to be replaced when it changes from yellow to light green.

[0073] The power density curve and polarization curve were determined by the variable resistance method. The external resistance was changed from large to small. The external resistance range was 100-10000Ω, and the external resistance values ​​were 10000Ω, 8000Ω, 6000Ω, 4000Ω, 2000Ω, 1000Ω, 800Ω, 600Ω, 400Ω, 200Ω, and 100Ω. The voltage was monitored using the Lanbo voltage test system. The system was run for 20-30 minutes at each resistance value. The stable voltage at each resistance value was recorded and the corresponding current was calculated.

[0074] The polarization curve is a curve drawn by different voltages at different current densities, calculated according to U = I × (r + R), where r (Ω) is the internal resistance of the stable and efficient power-generating microbial fuel cell, and R is the external resistance. The power density curve is drawn by different power densities at different current densities, calculated according to P = U 2 / (R×A) to calculate the power density, where R is the external resistor value, mA (m 2 ) is the anode electrode area. The maximum power density on the curve is defined as when R = r, P is the maximum power density.

[0075] Unless otherwise specified, all reagents used are commercially available reagents, and all detection means and methods used are conventional detection means and methods in the art.

[0076] Example 1

[0077] This embodiment provides a method for culturing enriched mixed electrogenic bacteria, which specifically includes the following steps:

[0078] (S1) After the activated sludge is allowed to stand for sealing, the supernatant is discarded, and then a 200-mesh screen is used to remove impurities, and an anolyte is added to immerse the sludge to provide nutrients required for microbial growth and metabolism to maintain a high activity of the microorganisms, thereby obtaining a mixed sludge with a concentration of 10 g / L, and then the mixed sludge is placed in a stirrer, sealed and stirred at room temperature for 3 days to obtain pretreated activated sludge;

[0079] (S2) using the pretreated activated sludge prepared in step (S1) as an inoculant (the volume ratio of the inoculant to the anolyte is 1:3), mixing it with the anolyte and placing it in the anode chamber of the first microbial fuel cell, and stably operating it for one cycle;

[0080] The reaction liquid in the anode chamber of the first microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 20 g / L sodium chloride, and placed in the anode chamber of the second microbial fuel cell, and stably operated for one cycle;

[0081] The reaction liquid in the anode chamber of the second microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 10 g / L sodium chloride, and placed in the anode chamber of the third microbial fuel cell, and stably operated for one cycle;

[0082] The reaction liquid in the anode chamber of the third microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 15 g / L sodium chloride, and placed in the anode chamber of the fourth microbial fuel cell, and stably operated for one cycle;

[0083] The reaction liquid in the anode chamber of the fourth microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 5 g / L sodium chloride and placed in the anode chamber of the fifth microbial fuel cell, and stably operated for one cycle to complete the anaerobic acclimation of the electrogenic bacteria in the activated sludge;

[0084] (S3) cutting the anode electrode containing the mixed electrogenic bacteria in the anode chamber of the fifth microbial fuel cell in step (S2) to obtain a 2 mm thick and 1 cm area anode electrode; 2 The anode electrode was used as the enrichment substrate; it was washed several times with phosphate buffer (50 mM, pH 7), and then transferred to a sterile anaerobic basal medium containing 15 mg / L phenazine-1-carboxylic acid and cultured at 30°C and 150 rpm until the bacterial solution OD 600 is 0.8, and an enriched mixed electrogenic bacteria 1 solution is obtained.

[0085] Comparative Example 1

[0086] This comparative example provides a method for culturing enriched mixed electrogenic bacteria, which specifically comprises the following steps:

[0087] (S1) After the activated sludge is allowed to stand for sealing, the supernatant is discarded, and then a 200-mesh screen is used to remove impurities, and an anolyte is added to immerse the sludge to provide nutrients required for microbial growth and metabolism to maintain a high activity of the microorganisms, thereby obtaining a mixed sludge with a concentration of 10 g / L, and then the mixed sludge is placed in a stirrer, sealed and stirred at room temperature for 3 days to obtain pretreated activated sludge;

[0088] (S2) using the pretreated activated sludge prepared in step (S1) as an inoculant (the volume ratio of the inoculant to the anolyte is 1:3), mixing it with the anolyte and placing it in the anode chamber of the first microbial fuel cell, and stably operating it for one cycle;

[0089] The reaction liquid in the anode chamber of the first microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 20 g / L sodium chloride, and placed in the anode chamber of the second microbial fuel cell, and stably operated for one cycle;

[0090] The reaction liquid in the anode chamber of the second microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 10 g / L sodium chloride, and placed in the anode chamber of the third microbial fuel cell, and stably operated for one cycle;

[0091] The reaction liquid in the anode chamber of the third microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 15 g / L sodium chloride, and placed in the anode chamber of the fourth microbial fuel cell, and stably operated for one cycle;

[0092] The reaction liquid in the anode chamber of the fourth microbial fuel cell is used as an inoculant, mixed with the anode liquid containing 5 g / L sodium chloride and placed in the anode chamber of the fifth microbial fuel cell, and stably operated for one cycle to complete the anaerobic acclimation of the electrogenic bacteria in the activated sludge;

[0093] (S3) cutting the anode electrode containing the mixed electrogenic bacteria in the anode chamber of the fifth microbial fuel cell in step (S2) to obtain a 2 mm thick and 1 cm area anode electrode; 2 The anode electrode was used as the enrichment substrate; it was washed several times with phosphate buffer (50 mM, pH 7), then transferred to sterile anaerobic basal medium and cultured at 30 ° C and 150 rpm until the bacterial solution OD 600 is 0.8, and an enriched mixed electrogenic bacteria 2 solution is obtained.

[0094] Example 2

[0095] This embodiment provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0096] In the anode chamber: 40 mL of the enriched mixed electrogenic bacteria 1 solution prepared in Example 1 was centrifuged at 6000 rpm for 8 min, the precipitated bacteria were resuspended with the anode liquid in the clean bench, and then added to the anode chamber and the anode liquid was replenished, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0097] In the cathode chamber: add 0.1 mol / L potassium ferrocyanide solution to obtain a high-efficiency electricity-generating microbial fuel cell: MFC-1.

[0098] The high-efficiency electricity-generating microbial fuel cell MFC-1 prepared in this example was placed in a constant temperature incubator at 30°C. The voltage was monitored using a Rambo voltage test system, and the voltage value was collected every 5 minutes and the data was automatically saved. When the voltage was lower than 50 mV, a cycle was completed.

[0099] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0100] The voltage variation diagram of MFC-1 in this embodiment is shown in FIG. Figure 1 As shown, the power density curve is as follows Figure 4 shown.

[0101] Comparative Example 2

[0102] This comparative example provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0103] In the anode chamber: 40 mL of the enriched mixed electrogenic bacteria 2 solution prepared in Comparative Example 1 was centrifuged at 6000 rpm for 8 min, the precipitated bacteria were resuspended with the anode liquid in the clean bench, and then added to the anode chamber and the anode liquid was replenished, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0104] In the cathode chamber: add 0.1 mol / L potassium ferrocyanide solution to obtain a highly efficient electricity-generating microbial fuel cell: MFC-2.

[0105] The high-efficiency electricity-generating microbial fuel cell: MFC-2 prepared in this comparative example was placed in a constant temperature incubator at a temperature of 30°C; the voltage was monitored using a Lanbo voltage test system, the voltage value was collected every 5 minutes and the data was automatically saved, and when the voltage was lower than 50m V, a cycle was completed.

[0106] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0107] The voltage variation diagram of MFC-2 in this comparative example is as follows: Figure 1 shown.

[0108] The output voltage of the high-efficiency electricity-generating microbial fuel cell prepared in Example 2 and Comparative Example 2 is as follows: Figure 1 As shown in the figure, the maximum stable voltage of MFC-1 is 752mV, and the maximum power density is 711.8mW·m 2 The stable power generation time is 125h, and the power generation cycle time is 264h. The maximum stable voltage of MFC-2 is 715mV, the stable power generation time is 132h, and the power generation cycle time is 264h. The voltage of Example 2 is higher than that of Comparative Example 2, and the time to reach the maximum stable voltage is shorter than that of Comparative Example 2.

[0109] Example 3

[0110] This embodiment provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0111] In the anode chamber: the enriched mixed electrogenic bacteria 1 solution in Example 1 and the pretreated activated sludge in Example 1 were mixed at a volume ratio of 1:1 to obtain a bacterial suspension 1; 40 mL of the bacterial suspension 1 was added to the anode chamber, and then supplemented with the anode liquid, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0112] In the cathode chamber: add 0.1 mol / L potassium ferrocyanide solution to obtain a highly efficient electricity-generating microbial fuel cell: MFC-3.

[0113] The high-efficiency electricity-generating microbial fuel cell MFC-3 prepared in this example was placed in a constant temperature incubator at 30°C. The voltage was monitored using a Rambo voltage test system, and the voltage value was collected every 5 minutes and the data was automatically saved. When the voltage was lower than 50 mV, a cycle was completed.

[0114] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0115] The voltage variation diagram of MFC-3 in this embodiment is shown in FIG. Figure 2 As shown, the power density curve is as follows Figure 4 shown.

[0116] Comparative Example 3

[0117] This comparative example provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0118] In the anode chamber: the enriched mixed electrogenic bacteria 2 solution in comparative example 1 and the pretreated activated sludge in comparative example 1 were mixed at a volume ratio of 1:1 to obtain a bacterial suspension 2; 40 mL of the bacterial suspension 2 was added to the anode chamber, and then supplemented with the anode liquid, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0119] In the cathode chamber: add 0.1 mol / L potassium ferrocyanide solution to obtain a highly efficient electricity-generating microbial fuel cell: MFC-4.

[0120] The high-efficiency electricity-generating microbial fuel cell: MFC-4 prepared in this comparative example was placed in a constant temperature incubator at a temperature of 30°C; the voltage was monitored using a Lanbo voltage test system, the voltage value was collected every 5 minutes and the data was automatically saved, and when the voltage was lower than 50m V, a cycle was completed.

[0121] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0122] The voltage variation diagram of MFC-4 in this comparative example is as follows: Figure 2 shown.

[0123] The voltage changes of the microbial fuel cells prepared in Example 3 and Comparative Example 3 are as follows: Figure 2 As shown in the figure, the maximum stable voltage of MFC-3 is 706mV, the stable power generation time is 115h, and the power generation cycle time is 235h. The maximum stable voltage of MFC-4 is 694mV, the stable power generation time is 120h, and the power generation cycle time is 246h. It can be seen that the voltage of Example 3 is higher than that of Comparative Example 3, and its startup time is slightly faster than that of Comparative Example 3. The power density curve of MFC-3 is shown in the figure Figure 4 As shown, through Figure 4 It can be found that the maximum power density of MFC-3 is 700.4mWm 2 .

[0124] Example 4

[0125] This embodiment provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0126] In the anode chamber: the enriched mixed electrogenic bacteria 1 solution in Example 1 and the pretreated activated sludge in Example 1 were mixed at a volume ratio of 1:1 to obtain a bacterial suspension 1; 40 mL of the bacterial suspension 1 was added to the anode chamber, and then supplemented with the anode liquid, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0127] In the cathode chamber: add 40mL of pretreated aerobic activated sludge, then make up with cathode liquid, put an aeration head in the cathode chamber, adjust the aeration volume so that the aeration head emits uniform small bubbles smoothly to provide oxygen to the cathode chamber, using oxygen and other substances as proton acceptors; obtain a high-efficiency electricity-generating microbial fuel cell: MFC-5.

[0128] The high-efficiency electricity-generating microbial fuel cell MFC-5 prepared in this example was placed in a constant temperature incubator at 30°C. The voltage was monitored using a Lanbo voltage test system, and the voltage value was collected every 5 minutes and the data was automatically saved. When the voltage was lower than 50m V, a cycle was completed.

[0129] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0130] The voltage variation diagram of MFC-5 in this embodiment is as follows: Figure 3 As shown, the power density curve is Figure 5 shown.

[0131] In this embodiment, the pretreated aerobic activated sludge is prepared by the following method:

[0132] After the activated sludge is allowed to stand for sealing, the supernatant is discarded, and then a 200-mesh screen is used to remove impurities, and cathode liquid is added to immerse the sludge to provide nutrients required for microbial growth and metabolism, maintain high microbial activity, and obtain a mixed sludge with a concentration of 4g / L. The activated sludge and wastewater are placed in a bucket at room temperature, and an aeration pump is added with an aeration flow rate of 6L / min. The sludge is cultured at room temperature for 10 days until the color of the mud water turns yellow (the sludge is aerated and acclimated), and the pretreated aerobic activated sludge is obtained.

[0133] The cathode liquid in this embodiment includes the following components in concentration:

[0134] 1.42g / L sodium nitrate, 2g / L sodium bicarbonate, 0.31g / L ammonium chloride, 0.13g / L potassium chloride, 2.88g / L potassium dihydrogen phosphate, 6.57g / L dipotassium hydrogen phosphate, the solvent is deionized water.

[0135] Comparative Example 4

[0136] This embodiment provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0137] In the anode chamber: the enriched mixed electrogenic bacteria 1 solution in comparative example 1 and the pretreated activated sludge in comparative example 1 were mixed at a volume ratio of 1:1 to obtain a bacterial suspension 2; 40 mL of the bacterial suspension 2 was added to the anode chamber, and then supplemented with the anode liquid, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0138] In the cathode chamber: add 40mL of pretreated aerobic activated sludge, then make up with cathode liquid, put an aeration head in the cathode chamber, adjust the aeration volume so that the aeration head emits uniform small bubbles smoothly to provide oxygen for the cathode chamber, using oxygen and other substances as proton acceptors; obtain a high-efficiency electricity-generating microbial fuel cell: MFC-6.

[0139] The high-efficiency electricity-generating microbial fuel cell: MFC-6 prepared in this comparative example was placed in a constant temperature incubator at a temperature of 30°C; the voltage was monitored using a Lanbo voltage test system, the voltage value was collected every 5 minutes and the data was automatically saved, and when the voltage was lower than 50m V, a cycle was completed.

[0140] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0141] The voltage variation diagram of MFC-6 in this comparative example is as follows: Figure 3 As shown, the power density curve is Figure 5 Shown

[0142] In this comparative example, the pretreated aerobic activated sludge was prepared by the following method:

[0143] After the activated sludge is allowed to stand for sealing, the supernatant is discarded, and then a 200-mesh screen is used to remove impurities, and an anolyte is added to immerse the sludge to provide the nutrients required for the growth and metabolism of microorganisms, maintain the high activity of microorganisms, and obtain a mixed sludge with a concentration of 4g / L. The activated sludge and wastewater are placed in a bucket at room temperature, and an aeration pump is added with an aeration flow rate of 6L / min. The sludge is cultured at room temperature for 10 days until the color of the mud water turns yellow (the sludge is aerated and acclimated), and the pretreated aerobic activated sludge is obtained.

[0144] The cathode liquid in this comparative example includes the following components in concentration:

[0145] 1.42g / L sodium nitrate, 2g / L sodium bicarbonate, 0.31g / L ammonium chloride, 0.13g / L potassium chloride, 2.88g / L potassium dihydrogen phosphate, 6.57g / L dipotassium hydrogen phosphate, the solvent is deionized water.

[0146] The voltage changes of the two groups of microbial fuel cells prepared in Example 4 and Comparative Example 4 are as follows: Figure 3 As shown, it can be seen that the voltage of the biocathode is significantly lower than that of the microbial fuel cell with potassium ferrocyanide solution as the cathode, and the biocathode MFC takes a long time to reach the maximum voltage, and the stable power generation time is lower than that of the MFC with potassium ferrocyanide solution as the cathode. The maximum stable voltage of MFC-5 (Example 4) is 452mV, and the maximum power density is 537.5mWm 2 , the stable power generation time is 104h, and the power generation cycle time is 158h. The maximum stable voltage of MFC-6 is 415mV, and the maximum power density is 505.1mWm 2 , the stable power generation time is 91h, and the power generation cycle time is 158h. And the startup time of MFC-5 is slightly lower than that of MFC-6.

[0147] Comparative Example 5

[0148] This comparative example provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0149] In the anode chamber: 40 mL of the pretreated activated sludge in Example 1 was added to the anode chamber, and then supplemented with anolyte, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0150] In the cathode chamber: add 0.1 mol / L potassium ferrocyanide solution to obtain a high-efficiency electricity-generating microbial fuel cell: MFC-7.

[0151] The high-efficiency electricity-generating microbial fuel cell MFC-7 prepared in this comparative example was placed in a constant temperature incubator at 30°C. The voltage was monitored using a Lanbo voltage test system, and the voltage value was collected every 5 minutes and the data was automatically saved. When the voltage was lower than 50m V, a cycle was completed.

[0152] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0153] The voltage variation diagram of MFC-7 in this comparative example is as follows: Figure 2 As shown, the power density curve is Figure 4 shown.

[0154] The voltage change diagram of MFC-7 is as follows Figure 2 As shown in the figure, its maximum stable voltage is 665mV, the stable power generation time is 102h, the power generation cycle time is 252h, and the maximum power of MFC-7 is 645.5mW·m 2 Compared with Example 3, the anode inoculum is only activated sludge, and the maximum stable voltage is relatively low. It can be concluded that the power generation voltage is related to the anode inoculum. The more electrogenic bacteria there are in the inoculum, the more electrons are generated by the electrogenic bacteria in the process of degrading organic matter, and the greater the power generation voltage of the microbial fuel cell.

[0155] Table 1 Power generation performance of MFC-3, MFC-4 and MFC-7

[0156]

[0157] Comparative Example 6

[0158] This embodiment provides a highly efficient electricity-generating microbial fuel cell, which is as follows:

[0159] In the anode chamber: 40 mL of the pretreated activated sludge in Example 1 was added to the anode chamber, and then supplemented with anolyte, and the anode chamber was subjected to nitrogen blowing and deoxygenation treatment;

[0160] In the cathode chamber: add 40mL of pretreated aerobic activated sludge, then make up with cathode liquid, put an aeration head in the cathode chamber, adjust the aeration volume so that the aeration head emits uniform small bubbles smoothly to provide oxygen for the cathode chamber, using oxygen and other substances as proton acceptors; obtain a high-efficiency electricity-generating microbial fuel cell: MFC-8.

[0161] The high-efficiency electricity-generating microbial fuel cell MFC-8 prepared in this example was placed in a constant temperature incubator at 30°C. The voltage was monitored using a Rambo voltage test system, and the voltage value was collected every 5 minutes and the data was automatically saved. When the voltage was lower than 50 mV, a cycle was completed.

[0162] When the voltage is lower than 50mV, two-thirds of the anode liquid is replaced and the microbial fuel cell is restarted to run for three cycles; the startup time is the time required from monitoring the voltage at the beginning of the operation to reaching the maximum stable voltage.

[0163] The voltage variation diagram of MFC-8 in this comparative example is as follows: Figure 3 As shown, the power density curve is Figure 5 shown.

[0164] In this comparative example, the pretreated aerobic activated sludge was prepared by the following method:

[0165] After the activated sludge is allowed to stand for sealing, the supernatant is discarded, and then a 200-mesh screen is used to remove impurities, and an anolyte is added to immerse the sludge to provide the nutrients required for the growth and metabolism of microorganisms, maintain the high activity of microorganisms, and obtain a mixed sludge with a concentration of 4g / L. The activated sludge and wastewater are placed in a bucket at room temperature, and an aeration pump is added with an aeration flow rate of 6L / min. The sludge is cultured at room temperature for 10 days until the color of the mud water turns yellow (the sludge is aerated and acclimated), and the pretreated aerobic activated sludge is obtained.

[0166] The cathode liquid in this comparative example includes the following components in concentration:

[0167] 1.42g / L sodium nitrate, 2g / L sodium bicarbonate, 0.31g / L ammonium chloride, 0.13g / L potassium chloride, 2.88g / L potassium dihydrogen phosphate, 6.57g / L dipotassium hydrogen phosphate, the solvent is deionized water.

[0168] The voltage results of MFC-8 in this comparative example are as follows Figure 3 The maximum stable voltage of MFC-8 is 362mV, the power generation cycle is 143h, and the maximum power density is 411.7mW·m 2 , the start-up time is 42h. It can be concluded that the power generation voltage is related to the anode inoculum and the cathode electron acceptor. The more electrogenic bacteria there are in the inoculum, the more electrons are generated by the electrogenic bacteria in the process of degrading organic matter, the faster the reduction reaction at the cathode, and the greater the power generation voltage of the microbial fuel cell.

[0169] Table 2 Power generation performance of MFC-3, MFC-4 and MFC-8

[0170]

[0171] pass Figure 1 It can be found that the maximum output voltage and power density of Example 2 are slightly higher than those of Comparative Example 2, and the MFC power generation performance is mainly related to the electrogenic bacteria, indicating that adding phenazine-1-carboxylic acid during the cultivation of electrogenic bacteria can promote their proliferation and inhibit the growth of miscellaneous bacteria, thereby improving the power generation performance.

[0172] pass Figure 2 It can be found that when the cathode liquid is 0.1 mol / L potassium ferrocyanide, the maximum output voltage and power density of Example 3 are slightly higher than those of Comparative Example 3. The anode inoculum of Comparative Example 5 (MFC-7) is only activated sludge, and the maximum output voltage is relatively low. It can be concluded that the power generation voltage is related to the anode inoculum. The more electrogenic bacteria there are in the inoculum, the more electrons are generated in the process of electrogenic bacteria degrading organic matter, and the greater the power generation voltage of the microbial fuel cell.

[0173] pass Figure 3 It can be found that the voltage of the biocathode is significantly lower than that of the microbial fuel cell with potassium ferrocyanide solution as the cathode, and the biocathode MFC takes a long time to reach the maximum voltage, and the stable power generation time is lower than that of the MFC with potassium ferrocyanide solution as the cathode.

[0174] pass Figure 4 It can be found that the maximum output power is: MFC-1>MFC-3>MFC-7. When the cathode liquid is 0.1 mol / L potassium ferrocyanide, the anode inoculum is different. The more the number of electrogenic bacteria in the inoculum, the greater the output voltage and maximum power density.

[0175] pass Figure 5 It can be found that the maximum output power is: MFC-5>MFC-6>MFC-8. When the cathodes are all biological cathodes, the more electrogenic bacteria there are in the inoculum, the greater the output voltage and maximum power density.

[0176] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the explanation of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A method for preparing a highly efficient electricity-generating microbial fuel cell, characterized in that: The following steps are involved: (S1) performing anaerobically acclimatization on the electrogenic bacteria in the activated sludge after screening and impurity removal to obtain mixed electrogenic bacteria; (S2) placing the mixed electrogenic bacteria prepared in step (S1) in an anaerobic basal culture medium containing phenazine-1-carboxylic acid for enrichment culture to obtain enriched mixed electrogenic bacteria; (S3) placing the enriched mixed electrogenic bacteria and the anolyte prepared in step (S2) in the anode chamber of a microbial fuel cell dual-chamber reactor, and then adding the cathode liquid into the cathode chamber to obtain a microbial fuel cell with high efficiency in generating electricity; Among them, in step (S1), during the anaerobic acclimation process, the salt tolerance of the electrogenic bacteria in the activated sludge is enhanced by using the stress pressure of jump salinity.

2. The method for preparing a high-efficiency electricity-generating microbial fuel cell according to claim 1, characterized in that: In step (S1), the anaerobic acclimation process is specifically as follows: (101) Using the activated sludge after sieving and removing impurities as an inoculant, mixing it with a carbon source and an anolyte, placing it in the anode chamber of the first microbial fuel cell, and stably operating it for one cycle; (102) using the reaction solution in the anode chamber of the first microbial fuel cell as an inoculant, mixing it with a carbon source and an anode solution containing 18 to 22 g / L of sodium chloride, placing it in the anode chamber of the second microbial fuel cell, and stably operating it for one cycle; (103) using the reaction solution in the anode chamber of the second microbial fuel cell as an inoculant, mixing it with a carbon source and an anode solution containing 8 to 12 g / L of sodium chloride, and placing it in the anode chamber of the third microbial fuel cell, and stably operating it for one cycle; (104) using the reaction liquid in the anode chamber of the third microbial fuel cell as an inoculant, mixing it with a carbon source and an anode liquid containing 13 to 17 g / L of sodium chloride, placing it in the anode chamber of the fourth microbial fuel cell, and operating it stably for one cycle; (105) using the reaction liquid in the anode chamber of the fourth microbial fuel cell as an inoculant, mixing it with a carbon source and an anode liquid containing 3 to 7 g / L of sodium chloride, and placing it in the anode chamber of the fifth microbial fuel cell, stably operating for one cycle to complete the anaerobic acclimation of the electrogenic bacteria in the activated sludge; The cathode liquid in the cathode chamber of the first microbial fuel cell, the second microbial fuel cell, the third microbial fuel cell, the fourth microbial fuel cell and the fifth microbial fuel cell is a 0.1-0.5 mol / L potassium ferrocyanide solution.

3. The method for preparing a high-efficiency electricity-generating microbial fuel cell according to claim 2, characterized in that: The volume ratio of the inoculant to the anolyte is 1:2 to 1:

4.

4. The method for preparing a highly efficient electricity-generating microbial fuel cell according to claim 2, characterized in that: In step (S2), the anode containing the mixed electrogenic bacteria in the anode chamber of the fifth microbial fuel cell is used as an enrichment substrate for enrichment culture.

5. The method for preparing a high-efficiency electricity-generating microbial fuel cell according to claim 1, characterized in that: In step (S2), the concentration of phenazine-1-carboxylic acid in the anaerobic basal medium containing phenazine-1-carboxylic acid is 10 to 30 mg / L; The anaerobic basal medium included the following components at the following concentrations: 10-16 g / L peptone, 5-10 g / L yeast extract, 4-10 g / L sodium chloride, 1 g / L starch, 1-3 g / L glucose, 1 g / L sodium pyruvate, 1 g / L arginine, 0.5 g / L sodium succinate, 0.4 g / L sodium bicarbonate, 0.5-1 g / L L-cysteine ​​hydrochloride, 0.5 g / L ferric pyrophosphate, 0.005 g / L hemoglobin, 0.0005-0.001 g / L vitamin K, 1 g / L DTT, 0.5 g / L sodium thioglycolate, and the solvent is deionized water.

6. The method for preparing a highly efficient electricity-generating microbial fuel cell according to claim 1, characterized in that: In step (S2), during the enrichment culture, the temperature is 15-40°C and the rotation speed is 50-300 rpm until the bacterial suspension OD 600 It is 0.6~1.

7. The method for preparing a highly efficient electricity-generating microbial fuel cell according to claim 1, characterized in that: The anolyte includes the following components in concentrations: 1.42g / L sodium acetate, 1-4g / L glucose, 6.57g / L potassium dihydrogen phosphate, 2.88g / L potassium dihydrogen phosphate, 0.2-0.4g / L ammonium chloride, 0.13g / L potassium chloride, 5-10mL / L vitamin solution, 10-15mL / L trace element solution, the solvent is deionized water; The cathode liquid is a 0.1-0.5 mol / L potassium ferrocyanide solution.

8. The method for preparing a high-efficiency electricity-generating microbial fuel cell according to claim 7, characterized in that: The vitamin solution includes the following components in the following concentrations: 5mg / L vitamin B1, 5mg / L vitamin B2, 5mg / L vitamin B3, 5mg / L vitamin B5, 10mg / L vitamin B6, 2mg / L vitamin B7, 2mg / L vitamin B9, 1mg / L vitamin B12, 5mg / L p-aminobenzoic acid, 5mg / L lipoic acid, the solvent is deionized water; The trace element solution includes the following components in concentration: 1.5g / L aminotriacetic acid, 1g / L sodium chloride, 3g / L MgSO4·7H2O, 0.5g / L MnSO4·H2O, 0.1g / L FeSO4·7H2O, 0.152g / L CoCl2·6H2O, 0.1g / L CaCl2·2H2O, 0.1g / L ZnSO4, 0.1g / L CuSO4·5H2O, 0.02g / L KAl(SO4)2·12H2O, 0.01g / L boric acid, 0.01g / LNa2MoO4·2H2O, 0.03g / L NiSO4·6H2O, 1mg / L Na2SeO3·5H2O, the solvent is deionized water.

9. A highly efficient electricity-generating microbial fuel cell, characterized in that: It is prepared by the method according to any one of claims 1 to 8.

10. Use of the high-efficiency electricity-producing microbial fuel cell according to claim 9 in the field of high-salinity wastewater treatment.

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