Spatial array electrode and method for detecting neurophysiological signals in the mitral cell layer of the olfactory bulb

Abstract

A spatial array electrode and method for acquiring neurophysiological signals from the olfactory bulb mitral cell layer are disclosed. In the spatial array electrode, an electrode bundle is implanted into the olfactory bulb of a rat. The electrode bundle includes multiple electrode bundles, each consisting of several electrode wires. The spatial height between the electrode wires is arranged according to the location of the mitral cell layer in the olfactory bulb region. A polyimide tube wraps around the electrode bundle to constrain its electrode wires, keeping the electrode wires parallel to each other. One end of the PCB interface is connected to the electrode bundle, and the other end is used to connect to the connector of an external acquisition device.

Description

Technical Field

[0001] This invention relates to the field of spatial array electrode technology, and in particular to a spatial array electrode and method for acquiring neurophysiological signals from the olfactory bulb mitral cell layer. Background Technology

[0002] The olfactory perception system, one of the primary sensory mechanisms by which rats explore the world, is a crucial brain region for neuroscientists studying how external odor molecules are converted into neural signals for odor recognition and classification. The olfactory bulb is the core of this system, and the mitral cell layer within it is a key region for olfactory signal processing and encoding. The mitral cells within this layer participate in the tuning and integration of odor signals through complex synaptic connections. Obtaining a large number of high-quality neurophysiological signals from the mitral cell layer helps scientists gain a deeper understanding of the mechanisms of olfactory information processing. However, the mitral cell layer in the olfactory bulb is a spherical, ribbon-like structure only 100 μm thick, yet its function in olfactory signal processing and transmission is vital. This small thickness makes acquiring electrical signals in this region extremely difficult and challenging.

[0003] Currently, the electrodes available on the market for neurophysiological acquisition mainly include microfilament array electrodes, silicon-based electrodes, and various flexible electrodes. However, using these electrodes to acquire neural electrical signals from the olfactory bulb's mitral cell layer is not a good choice. For example, most microfilament array electrodes are typically two-dimensional planar array electrodes without spatial array arrangement, and can only acquire neural electrical signals from the dorsal or ventral unilateral mitral cell layer, failing to obtain comprehensive information on mitral cell neural electrical activity. Besides microfilament array electrodes, high-channel, high-density silicon-based electrodes and flexible electrodes are also widely used for acquiring neurophysiological signals. However, high-density channels are only linearly and equidistantly arranged in space. After implantation in the olfactory bulb, most channels will fall into other non-mitral cell layers such as the granule cell layer, resulting in a relatively small number of channels actually acquiring mitral cell layer signals, thus wasting most of the channel count.

[0004] The information disclosed in the background section is only intended to enhance the understanding of the background of the present invention, and therefore may contain information that does not constitute prior art known to those skilled in the art. Summary of the Invention

[0005] To address the shortcomings or defects of the existing technology, a spatial array electrode and method for acquiring neurophysiological signals from the olfactory bulb mitral cell layer are provided. This method can maximize the use of all acquisition channels and simultaneously acquire neurophysiological signals from the dorsal and ventral mitral cell layers of the olfactory bulb.

[0006] The objective of this invention is achieved through the following technical solutions.

[0007] A spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer includes,

[0008] The electrode bundle includes multiple electrode bundles, each electrode bundle consisting of several electrode wires, with the spatial height between the electrode wires arranged according to the location of the mitral cell layer in the olfactory bulb region.

[0009] A polyimide tube that wraps a bundle of electrodes to constrain the electrode wires, keeping the electrode wires parallel to each other.

[0010] The interface has one end connected to the electrode bundle and the other end used to connect to the connector of an external acquisition device.

[0011] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the electrode bundle contains 16 polyimide tubes arranged in a 4×4 array. Each polyimide tube contains 4 electrode wires, for a total of 4×4×4 electrode microwires, to collect 64 channels of electrical signals.

[0012] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the polyimide tube has a diameter of 0.15 mm.

[0013] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, four electrode wires are arranged as follows: the four electrode wires are divided into two layers, upper and lower, each layer containing two electrode wires, with a spacing of 3.5 mm between the two layers, and a floating distance of 100 μm between the two electrode wires in the upper or lower layer.

[0014] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the electrode wire has a diameter of 35 μm, is made of nickel-chromium alloy, is covered with an acetylimine insulating layer, and has exposed metal tip contacts.

[0015] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the interface is divided into J1 and J2 surfaces, each with 32 signal acquisition channels.

[0016] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, an insulating layer is provided on the surface of the PCB interface.

[0017] In the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the insulating layer is formed by covering with AB glue.

[0018] The preparation method of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer includes the following steps.

[0019] Cut a polyimide tube and four electrode wires, two of which are 30 mm long and the other two are 25 mm long. Fix the polyimide tube on a smooth horizontal surface and insert the four electrode wires into the polyimide tube. Then pull the contact section of the four electrode wires to 5 mm and keep the tips of the four contact sections flush. Use tweezers to adjust the contact distance between the two 25 mm electrode wires at 5 mm to 100 μm. Then use tweezers to pull the two 30 mm electrode wires back to 1.5 mm and adjust the contact distance between the two electrode wires to 100 μm. After adjusting the spatial arrangement of the four electrode wires, apply glue to the entrance of the polyimide tube to fix the spatial arrangement of the four electrode wires and form an electrode bundle.

[0020] After completing the spatial arrangement of 16 single electrode bundles, the 16 single electrode bundles are divided into 8 groups, with two single electrode bundles in each group. Glue is applied to the polyimide tubes to bond the two single electrode bundles together in parallel. The 8 groups of single electrode bundles are then bonded together in pairs to form four groups, each containing 4 single electrode bundles. The 4 groups of single electrode bundles are stacked upwards in 4 layers, and glue is applied to the polyimide tubes to keep the 4×4 spatial array fixed to form the motor bundle.

[0021] After scraping off the insulation layer of the top connecting section of the 64 conductive electrode wires, use glue to fix the upper polyimide tube to the upper half of the blank space of the notch on the PCB substrate. Fill the notch with the remaining lower end. The 16 polyimide tubes are divided into 8 tubes on the J1 side and 8 tubes on the J2 side. All 25 mm long electrode wire connecting sections of the 8 polyimide tubes on the J1 side are soldered to the left pad contact on the J1 side, and the 30 mm long electrode wire connecting sections are soldered to the right pad contact on the J1 side.

[0022] In the method described, AB glue is applied to the pads on the J1 and J2 surfaces, and a hard protective layer is formed after the AB glue solidifies.

[0023] Compared with existing technologies, the beneficial effects of this invention are as follows: the spatial array electrode of this invention can simultaneously acquire neurophysiological signals from the dorsal and ventral mitral cell layers of the rat olfactory bulb, thus enabling greater flexibility in signal acquisition from the rat olfactory mitral cell layer. This overcomes the shortcomings of existing electrodes, such as their inability to obtain comprehensive information on the neuroelectrical activity of mitral cells, and the fact that most channels are located in other non-mitral cell layers such as the granule cell layer.

[0024] The description provided is merely an overview of the technical solution of this invention. In order to make the technical means of this invention clearer and more understandable, so that those skilled in the art can implement it according to the contents of the specification, and to make the described and other objects, features and advantages of this invention more obvious and understandable, specific embodiments of this invention are described below. Attached Figure Description

[0025] Various other advantages and benefits of the present invention will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiments below. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. It is obvious that the drawings described below are merely some embodiments of the invention, and those skilled in the art can obtain other drawings based on these drawings without any inventive effort. Furthermore, the same reference numerals denote the same parts throughout the drawings.

[0026] In the attached diagram:

[0027] Figure 1 This is a schematic diagram of the structure of a spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, according to an embodiment of the present invention.

[0028] Figure 2 This is a physical diagram of the overall structure of a spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, provided in one embodiment of the present invention.

[0029] Figure 3 A schematic diagram of the overall spatial structure and size of a spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, provided in an embodiment of the present invention;

[0030] Figure 4 This is a schematic diagram of the spatial array electrode 4-lead single-bundle spatial 3D arrangement for collecting neurophysiological signals from the olfactory bulb mitral cell layer, provided in one embodiment of the present invention.

[0031] Figure 5 A 3D schematic diagram of a spatial array electrode array of 16 electrode bundles arranged in a 4×4×4 spatial arrangement for collecting neuroelectrophysiological signals of the olfactory bulb mitral cell layer according to an embodiment of the present invention;

[0032] Figure 6 This is a schematic diagram of the PCB interface J1 and J2 surfaces of a spatial array electrode for acquiring neuroelectrophysiological signals from the olfactory bulb mitral cell layer, according to an embodiment of the present invention.

[0033] Figure 7 This is a schematic diagram of the neural electrical signals collected by a spatial array electrode for collecting neural electrophysiological signals of the olfactory bulb mitral cell layer, according to an embodiment of the present invention. The signal includes 64 channels of local field potentials and representative neural pulse signals.

[0034] The present invention will be further explained below with reference to the accompanying drawings and embodiments. Detailed Implementation

[0035] Specific embodiments of the invention will now be described in more detail with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it should be understood that the invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the invention and to fully convey the scope of the invention to those skilled in the art.

[0036] It should be noted that certain terms are used in the specification and claims to refer to specific components. Those skilled in the art will understand that different terms may be used to refer to the same component. This specification and claims do not distinguish components based on differences in terminology, but rather on differences in function. The terms "comprising" or "including" used throughout the specification and claims are open-ended and should be interpreted as "comprising but not limited to." The following descriptions are preferred embodiments for carrying out the invention; however, these descriptions are for the purpose of understanding the general principles of the specification and are not intended to limit the scope of the invention. The scope of protection of this invention is determined by the appended claims.

[0037] To facilitate understanding of the embodiments of the present invention, the following will provide further explanation and description with reference to the accompanying drawings and several specific embodiments, and the accompanying drawings do not constitute a limitation on the embodiments of the present invention.

[0038] To better understand, such as Figures 1 to 7 As shown, a spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer includes,

[0039] An electrode bundle, which can be implanted into suitable objects such as the olfactory bulb of a rat, comprises multiple electrode bundles, each consisting of several electrode wires. The spatial arrangement of the electrode wires corresponds to the location of the mitral cell layer in the olfactory bulb region. Specifically, the difference in mitral cell layer between the dorsal and ventral sides of the rat olfactory bulb is approximately 3.5 mm. The four electrodes are divided into upper and lower layers at 3.5 mm intervals. The lower layer corresponds to the ventral mitral cell layer, and the maximum positioning distance of its electrode contact is 4.1-4.2 mm. The overall length of the electrode is 5 mm. The positions of the upper two electrode contacts are 1.5 mm from the electrode origin. When the electrode is implanted into the rat olfactory bulb, the lower electrode contacts are positioned at 4.1-4.2 mm, while the upper two electrode contacts will fall at 600-800 μm in the olfactory bulb, corresponding to the location of the dorsal mitral cell layer. Furthermore, due to individual and tissue differences, the positions of the dorsal and ventral capillary cell layers may vary to some extent. In order to maximize contact with the capillary cell layers, the two conductive electrode contacts within the two layers will be spaced 100 μm apart.

[0040] A polyimide tube that wraps a bundle of electrodes to constrain the electrode wires, keeping the electrode wires parallel to each other.

[0041] The PCB interface has one end connected to the electrode bundle and the other end used to connect to the connector of an external acquisition device.

[0042] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the electrode bundle has 16 polyimide tubes arranged in a 4×4 array. Each polyimide tube contains 4 electrode wires, for a total of 4×4×4 electrode microwires, to collect 64 channels of electrical signals.

[0043] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the polyimide tube has a diameter of 0.15 mm.

[0044] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the four electrode wires are arranged as follows: the four electrode wires are divided into two layers, upper and lower, each layer containing two electrode wires, with a spacing of 3.5 mm between the two layers, and a floating distance of 100 μm between the two electrode wires in the upper or lower layer.

[0045] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the electrode wire has a diameter of 35 μm, is made of nickel-chromium alloy, is covered with an acetylimine insulating layer, and has exposed metal tip contacts.

[0046] In a preferred embodiment of the spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer, the PCB interface is divided into a J1 side and a J2 side, each side having 32 channels of signal acquisition lines.

[0047] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, an insulating layer is provided on the surface of the PCB interface.

[0048] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer, the insulating layer is formed by covering with AB glue.

[0049] The preparation method of the spatial array electrode for collecting neurophysiological signals from the olfactory bulb mitral cell layer includes the following steps.

[0050] Cut a polyimide tube and four electrode wires, two of which are 30 mm long and the other two are 25 mm long. Fix the polyimide tube on a smooth horizontal surface and insert the four electrode wires into the polyimide tube. Then pull the contact section of the four electrode wires to 5 mm and keep the tips of the four contact sections flush. Use tweezers to adjust the contact distance between the two 25 mm electrode wires at 5 mm to 100 μm. Then use tweezers to pull the two 30 mm electrode wires back to 1.5 mm and adjust the contact distance between the two electrode wires to 100 μm. After adjusting the spatial arrangement of the four electrode wires, apply glue to the entrance of the polyimide tube to fix the spatial arrangement of the four electrode wires and form an electrode bundle.

[0051] After completing the spatial arrangement of 16 single electrode bundles, the 16 single electrode bundles are divided into 8 groups, with two single electrode bundles in each group. Glue is applied to the polyimide tubes to bond the two single electrode bundles together in parallel. The 8 groups of single electrode bundles are then bonded together in pairs to form four groups, each containing 4 single electrode bundles. The 4 groups of single electrode bundles are stacked upwards in 4 layers, and glue is applied to the polyimide tubes to keep the 4×4 spatial array fixed to form the motor bundle.

[0052] After scraping off the insulation layer of the top connecting section of the 64 conductive electrode wires, use glue to fix the upper polyimide tube to the upper half of the blank space of the notch on the PCB substrate. Fill the notch with the remaining lower end. The 16 polyimide tubes are divided into 8 tubes on the J1 side and 8 tubes on the J2 side. All 25 mm long electrode wire connecting sections of the 8 polyimide tubes on the J1 side are soldered to the left pad contact on the J1 side, and the 30 mm long electrode wire connecting sections are soldered to the right pad contact on the J1 side.

[0053] In a preferred embodiment of the method, AB adhesive is applied to the pads on surfaces J1 and J2, and a hard protective layer is formed after the AB adhesive solidifies.

[0054] In one embodiment, the spatial structure and size of the spatial array electrodes for acquiring neurophysiological signals from the olfactory bulb mitral cell layer are determined based on a rat brain localization atlas. Specifically, combined with Figure 3According to the 6th edition of the Rat Brain Localization Atlas, the olfactory bulb is located 16.08 mm from the interauricular distance and 7.08 mm from the anterior fontanelle (AP: 7.08), which is on the second page of the brain localization atlas. A 1 mm × 1 mm rectangular window is drawn 0.6 mm lateral to the sagittal suture. Based on the brain atlas, the dorsal mitral cell layer of the olfactory bulb is approximately 600-700 μm below the dura mater, and the maximum distance between the two dorsal electrode contacts is located at 700 μm below the dura mater. Due to individual and tissue differences, the location of the dorsal mitral cell layer may vary slightly. To maximize contact with the dorsal mitral cell layer, the two dorsal electrode contacts are spaced 100 μm apart.

[0055] The ventral mitral cell layer is located at approximately 4.2 mm. The maximum positioning distance between the electrode contacts on the two ventral leads is 4.1-4.2 mm. Due to individual and tissue differences, the position of the ventral mitral cell layer may vary slightly. To maximize contact with the ventral mitral cell layer, the electrode contacts on the two ventral leads are spaced 100 μm apart.

[0056] Based on the approximately 3.5 mm difference in the dorsal and ventral mitral cell layers of the rat olfactory bulb, the distance between the two conductive electrode contacts on the dorsal side and the two conductive electrode contacts on the ventral side is 3.5 mm.

[0057] The overall length of the electrode is 5 mm. The two dorsal electrode contacts are located 1.5 mm from the electrode starting point. When implanted, the ventral electrode contacts are positioned at 4.1-4.2 mm, and the two dorsal electrode contacts will fall at 600-800 μm in the olfactory bulb, corresponding to the location of the dorsal mitral cell layer.

[0058] In the embodiments of this application, combined with Figure 4 The arrangement of a single bundle of 4-channel electrode wires includes the following steps.

[0059] Specifically, a polyimide tube with a length of approximately 10 mm and a diameter of 0.15 mm is cut, and four electrode wires are cut using special scissors, two of which are 30 mm long and the other two are 25 mm long.

[0060] The individual polyimide tubes were fixed to a smooth horizontal surface with medical tape. Using a high-powered microscope and a standard scale, the four electrode wires were first inserted into the polyimide tubes. Then, the contact sections of the four electrode wires were pulled to 5 mm, and the tips of the four contact sections were kept flush.

[0061] After completing the above operations, use fine tweezers to adjust the contact distance between the two 25 mm electrode wires at 5 mm to 100 μm.

[0062] After completing the above operations, use fine tweezers to pull the two 30 mm electrode wires back to 1.5 mm, and adjust the contact distance between the two electrode wires to 100 μm according to the design requirements.

[0063] After adjusting the spatial arrangement of the four electrode wires, use a toothpick to apply a small amount of glue to the inlet of the polyimide tube to fix the spatial arrangement distance of the four electrode wires.

[0064] In the embodiments of this application, combined with Figure 5 The arrangement of the 16 arrays involves the following steps.

[0065] Specifically, after completing the spatial arrangement of 16 single-bundle electrode clusters according to the above operations, in order to form a 4×4 array electrode, the 16 electrode clusters are first divided into 8 groups, with two single bundles in each group. A small amount of glue is applied to the polyimide tubes to bond the two bundles together in parallel. The 8 groups of electrode clusters are then bonded together in pairs in parallel, thus forming four groups, each containing 4 single-bundle electrode clusters. The 4 groups of electrode clusters are then stacked upwards in 4 layers, and a small amount of glue is applied to the polyimide tubes to keep the 4×4 spatial array fixed.

[0066] In the embodiments of this application, combined with Figure 6 The welding method for electrodes and PCB boards includes the following steps.

[0067] Specifically, once the above steps are completed, the connection segment, polyimide, and contact segment of the 4×4×4 spatial array electrode suitable for collecting neurophysiological signals from the olfactory bulb mitral cell layer of rats are completed.

[0068] The aforementioned 64 conductive wires in the connecting section all have an insulating coating. To enable signal conduction, the insulating layer at the top of the connecting section needs to be scraped off using tweezers. After completely scraping off the insulating layer at the top of the 64 conductive wire connecting section, the three parts that have been fixed above are treated as a whole. The upper polyimide tube is fixed to the upper half of the blank space at the notch on the PCB substrate shown in the figure below with glue, and the remaining lower end is filled into the notch, thus fixing the three parts together on the PCB substrate.

[0069] Using a soldering gun and solder, electrode wires with the insulation removed are soldered onto the pads of the PCB circuit board. Specifically, using a high-precision microscope, 16 polyimide tubes are divided into 8 tubes on side J1 and 8 tubes on side J2. All 25 mm long electrode wire connection segments of the 8 polyimide tubes on side J1 are soldered to the left pad contact on side J1, and the 30 mm long electrode wire connection segments are soldered to the right pad contact on side J1. After soldering, the electrode wires on the pads are tidied up, and two silver wires are soldered to the ground lines and reference pad contacts on both sides of side J1.

[0070] Similarly, all 25 mm long electrode wire connection segments of the 8 polyimide tubes on the J2 side are soldered to the left pad contact on the J2 side, and the 30 mm long electrode wire connection segments are soldered to the right pad contact on the J2 side.

[0071] After all 64 conductive wires are arranged and soldered, AB glue is applied to the pads on the J1 and J2 sides. Once the AB glue has solidified, a hard protective layer is formed.

[0072] In the embodiments of this application, the steps for using the spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer and the neurophysiological acquisition process include the following steps.

[0073] Specifically, rats weighing 250-290 g were selected, anesthetized with 4% isoflurane, the hair on the rat's head was removed, and the rat was immediately fixed on a stereotaxic instrument. The anesthesia concentration was maintained at 1.5-2%, the air flow rate was 500-700 ml / min, and finally erythromycin eye ointment was applied to the rat's eyes to keep them moist.

[0074] Clean the surgical incision site in the order of 75% alcohol, iodine, and then 75% alcohol. Remove the skin and muscle tissue between the two ears and the two eyes on the top of the rat's skull with scissors to fully expose the hard bone layer. After the skull surface dries, the sagittal suture, anterior fontanelle, and posterior fontanelle will be clearly visible. At this point, use a clamp to vertically fix the skull drill and move the tip of the skull drill to the front end of the sagittal suture. Move it slowly backward, ensuring that the tip stays on the sagittal suture throughout the movement and that the anterior and posterior distances from the skull surface are similar. This proves that the rat's head is fixed in accordance with the positioning requirements.

[0075] Based on the rat brain localization atlas, the location of the rat olfactory bulb was determined to be 16.08 mm from the interauricular distance, 7.08 mm from the anterior fontanelle (AP: 7.08), and 0.6 mm lateral to the sagittal suture (ML: 0.6). After setting the above parameters using an automated brain localization instrument, a 1 mm × 1 mm rectangular window was drilled in the left or right olfactory bulb using a cranial drill. The dura mater inside the rectangular window was then dissected using precision forceps. A small hole was also made below the contralateral olfactory bulb as the implantation point for the reference electrode. Another small hole was made in a brain region closer to the olfactory bulb as the implantation point for the ground wire.

[0076] When using this spatial array electrode to collect neurophysiological signals from the rat olfactory bulb mitral cell layer, the electrode tip was first immersed in anhydrous ethanol and then in 75% alcohol to clean and disinfect the electrode tip.

[0077] Fix the electrode base to the precision brain localizer using the electrode holder, adjust the angle so that the direction of the electrode wire is perpendicular to the plane of the skull, connect it to the input terminal of the signal acquisition system, slowly bring the electrode close to the cranial window, put the reference line and ground line of the electrode into the pre-drilled holes respectively, and apply special tissue adhesive to fix the ground line and reference line.

[0078] Clean the rectangular window that has been positioned in the olfactory bulb. After lowering the tip of the electrode to the brain surface using a precision stereotaxic instrument, set all spatial parameters of the stereotaxic instrument to 0. Implant the tip of the electrode at a position 4.1-4.2 mm below the dura mater of the olfactory bulb (DV: 4.1-4.2 mm).

[0079] A small amount of bleeding was absorbed using a medical cotton ball, and a special tissue adhesive was dripped into the cranial window to initially fix the electrode array. Then, a thinner dental cement was used to flow into the gaps of the excess electrode array at the top to further fix the electrode array. The tissue adhesive was then dripped onto the entire surgical wound. After it solidified, the entire exposed skull and the upper end of the electrodes were sealed with dental cement. After it had completely solidified, the anesthesia was turned off, the rat was removed from the locator, and placed on a heating pad until it recovered. Once it could move independently, it was placed in a cage for individual rearing.

[0080] After waiting 2-3 days, it can be used for the acquisition of neural electrical signals, combined with... Figure 7 The local field potential recordings detected by this application have high stability and a high yield rate. The detected neural impulse signals have good waveform shape and a high signal-to-noise ratio.

[0081] This spatial array electrode is used to acquire neurophysiological signals from the mitral cell layer of the rat olfactory bulb. It can simultaneously acquire neurophysiological signals from both the dorsal and ventral mitral cell layers of the rat olfactory bulb, providing greater flexibility in signal acquisition and maximizing the utilization of the available channels to obtain a larger number of neuronal information channels, avoiding the waste of most channels. This application overcomes the shortcomings of current commercially available electrodes, which cannot obtain comprehensive neurological activity information from mitral cells, and whose majority of channels are located in granular cells or other non-mitral cell layers.

[0082] The basic principles of this application have been described above with reference to specific embodiments. However, it should be noted that the advantages, benefits, and effects mentioned in this application are merely examples and not limitations, and should not be considered as essential features of each embodiment of this application. Furthermore, the specific details disclosed above are for illustrative and facilitative purposes only, and are not limitations. These details do not limit the application to the necessity of employing the aforementioned specific details for implementation.

[0083] The above description has been given for purposes of illustration and description. Furthermore, this description is not intended to limit the embodiments of this application to the forms disclosed herein. Although numerous exemplary aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, alterations, additions, and sub-combinations thereof.

Claims

1. A spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer, characterized in that, It includes, The electrode bundle comprises multiple electrode bundles, each consisting of several electrode wires. The spatial arrangement of the electrode wires is based on the location of the mitral cell layer in the olfactory bulb region. The electrode bundle includes 16 polyimide tubes. Four electrode wires are arranged in two layers, upper and lower, with two electrode wires in each layer. The distance between the two layers is 3.5 mm, and there is a 100 μm floating distance between the two electrode wires in the upper or lower layer. The electrode wires have a diameter of 35 μm, are made of nickel-chromium alloy, and are covered with an acetylimide insulating layer, with their metal tip contacts exposed. A polyimide tube is used to wrap a bundle of electrodes to constrain the electrode wires and keep the electrode wires parallel to each other; wherein the diameter of the polyimide tube is 0.15 mm. The interface has one end connected to the electrode bundle and the other end used to connect to the connector of an external acquisition device.

2. The spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer as described in claim 1, characterized in that, The interface is divided into J1 and J2 surfaces, each with 32 channels of signal acquisition lines.

3. The spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer as described in claim 1, characterized in that, An insulating layer is provided on the surface of the interface.

4. The spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer as described in claim 3, characterized in that, The insulating layer is formed by covering with AB glue.

5. The method for preparing a spatial array electrode for acquiring neurophysiological signals from the olfactory bulb mitral cell layer as described in any one of claims 1-4.

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