A painless and efficient composite bioenzyme tattoo remover, its preparation method and its application in tattoo removal

Abstract

The invention discloses a painless and efficient composite bioenzyme tattoo remover, a preparation method thereof and an application thereof in tattoo removal, wherein the tattoo remover comprises a composite bioenzyme, a permeating agent and an auxiliary agent. One or more of laccase, cysteine ​​desulfurase, oxide dismutase and catalase; the permeating agent comprises one or more of azone, dimethyl isosorbide, N-n-alkyl benzisothiazone or oxazolidinone, and cyclohexanehexol; the auxiliary agent comprises one or more of gelatin, anhydrous citric acid, sodium gluconate, ethanol, tea polyphenols, grape seed anthocyanin, vitamin C and urea. The preparation is colorless, tasteless, non-toxic and has no side effects. The ingredients are pure natural organic enzymes, permeating agents and auxiliary agents, all of which are of medical grade and cosmetic grade, and are safe and reliable. Tattoos can be removed by using the preparation provided by the invention without pain, swelling, allergy, adverse reaction or sequelae.

Description

Technical Field

[0001] The invention belongs to the technical field of medical cosmetology, and in particular relates to a painless and efficient composite bio-enzyme tattoo remover, a preparation method thereof, and application thereof in tattoo removal. Background Art

[0002] Tattoos are usually made by piercing the bottom layer of the skin with a needle containing ink to create some patterns or fonts on the skin. In terms of tattoo removal technology, surgery, chemical peeling, laser cleaning, etc. are generally used. Laser technology uses the energy released instantly by laser to act directly on the local area. Lasers of different colors are absorbed by the skin of different colors, breaking the pigment particles into extremely small fragments, decomposing and destroying them. Through skin metabolism, they are excreted from the body. The pigment will fade until it disappears, but complete elimination usually requires multiple treatments. At present, laser therapy is also the world's only successful technology. However, in the process of using laser cleaning, it brings great pain to the tattooer, and has the problems of expensive treatment, incomplete removal, and most of the scars left.

[0003] The main colors of tattoos are:

[0004] Red: The main component of red is mercuric sulfide, which is commonly known as cinnabar. Other pigments include organic pigments, such as magenta and carmine. Red mercuric sulfide can cause itching, eczematous granulomas, sarcoid reactions, etc. People who receive red tattoos usually live in peace with them for many years. However, the above changes will suddenly occur when the body is stimulated by some kind of stimulation, such as abrasions on the tattooed area, or vaccinations containing mercury.

[0005] Green: The most common ingredient is chromium oxide or hydrated chromium oxide. This pigment is quite stable, acid-resistant, and insoluble in water, alcohol, and acetone. Other pigments include emerald green, copper oxide, and chromium trioxide. The skin reaction caused by green chromium oxide is mainly eczema. Some people may develop systemic eczema due to tattooing with green pigment. The tattoo must be removed for the eczema to be cured.

[0006] Blue: The main component is cobalt aluminate, and other pigments are indigo and phthalocyanine. The reactions caused by blue cobalt aluminate include inflammatory reactions and sarcoid-like allergic reactions, and sometimes even the adjacent lymph nodes can also see similar changes.

[0007] Yellow: The common ingredient is cadmium sulfide, but sometimes this ingredient can also be found in red reagents. Other pigments include lemon yellow and chromium zinc oxide ferric hydrate. Chemists have found that some yellow colors will decompose when exposed to sunlight or certain enzymes, but it is not clear whether it is toxic. After being exposed to the sun, the tattoo area will feel itchy and red and swollen.

[0008] Purple: The main component is manganese. The reaction caused by purple manganese is itching and swelling. Black: The main components are hematoxylin and black ink.

[0009] Brown: Mainly cadmium salts and Venetian red.

[0010] White: The commonly used ingredient is zinc oxide.

[0011] From the above analysis, we can know that the pigments of tattoos are mainly metals, semi-metals or organic compounds. After these substances penetrate the epidermis, some people with special physiques will find skin symptoms, especially red, green, blue, yellow and purple, which are more likely to cause reactions.

[0012] Therefore, there is an urgent need for a tattoo removal technology with good effect, low cost and high safety. Summary of the invention

[0013] In view of the deficiencies of the prior art, the present invention provides a painless and efficient composite bio-enzyme tattoo remover, a preparation method thereof and its application in tattoo removal. The technical solution implemented by the present invention is:

[0014] In a first aspect, the present invention provides a painless and efficient composite bio-enzyme tattoo remover, comprising a composite bio-enzyme, wherein the composite bio-enzyme comprises the following components:

[0015] Laccase: 1-3 parts; cysteine ​​desulfurase: 1-2.5 parts; oxide dismutase: 1-3 parts; one or more of catalase 1-3 parts.

[0016] Preferably, laccase is 1-2.5 parts, cysteine ​​desulfurase is 1-2 parts, oxide dismutase is 1-2.5 parts, and catalase is 1-2.5 parts.

[0017] Furthermore, the tattoo remover further comprises a penetrant, and the penetrant comprises the following components:

[0018] One or more of azone: 1-3.5 parts; dimethyl isosorbide: 1-3 parts; N-n-alkylbenzisothiazine or oxazolidinone: 1-2 parts; cyclohexanehexol: 1-2.5 parts.

[0019] Preferably, the azone is lauryl azone, and the tattoo remover contains 1-2.5 parts of lauryl azone.

[0020] Preferably, the tattoo remover comprises 1.5 parts of dimethyl isosorbide and 1-1.5 parts of N-n-alkylbenzisothiazolone or oxazolidinone.

[0021] Also contains Tetrahydrocannabinol: 1-2 servings.

[0022] Furthermore, the tattoo remover further comprises an auxiliary agent, and the auxiliary agent comprises the following components: one or more of gelatin, urea, citric acid, sodium gluconate, ethanol, tea polyphenols, grape seed anthocyanin, and vitamin C.

[0023] Furthermore, the auxiliary agent includes gelatin: 1-5 parts; anhydrous citric acid: 1-2 parts; sodium gluconate: 1-3 parts; ethanol: 1-5 parts; tea polyphenols: 0.5-2 parts; grape seed anthocyanin: 0.5-3 parts; vitamin C: 1-1.5 parts; urea: 1-5 parts.

[0024] Furthermore, the amount of gelatin is 1-3 parts.

[0025] Furthermore, it also contains 1-80 parts of distilled water.

[0026] The functions of each component are described as follows:

[0027] (1) Laccase: Laccase is a polyphenol oxidase containing four copper ions (diphenol oxidase, ECI.10.3.2), which belongs to copper blue oxidase and exists in the form of monomeric glycoprotein. Laccase exists in mushrooms, fungi and plants, and can also survive in the air. The only product after the reaction is water, so it is essentially an environmentally friendly enzyme. Laccase is a copper protein, blue, with a molecular weight of about 120,000, and contains four atoms of copper. Laccase can oxidize polyphenols, promote methoxy substitution of phenol and diamine, and oxidize almost all substrates with similar structures to polyphenols. In addition, some fungal laccases can also oxidize monophenols such as cresol and ascorbic acid. At present, the application research of laccase in China is mainly focused on decomposing lignin, pulp bleaching, decolorizing dye wastewater, and degrading toxic substances in the environment. Laccase has a strong ability to degrade phenolic compounds. Immobilizing it on an organic gel carrier can remove aromatic compounds in the water body and those mixed from the outside from the suspended liquid. The precipitate produced by the laccase substrate after oxidation coupling is insoluble in industrial wastewater and is also conducive to the thorough removal of phenolic substances. Therefore, most of the pigments in tattoos, such as indigo, benzoindole, hematoxylin, black water and other organic substances and azo pigments, can be completely removed by coupling to produce precipitation.

[0028] (2) Cysteine ​​desulfurase: L-cysteine ​​is a common amino acid in living organisms. It is purple and exists in many proteins and glutathione. + , Hg + , Cu +Metal ions such as thiols can form insoluble thiols. Molecular formula C3H7N02S, molecular weight 121.6, colorless crystals. Soluble in water, ethanol, acetic acid and ammonia, insoluble in ether, acetone, ethyl acetate, benzene, carbon disulfide and carbon tetrachloride. It can be oxidized to cystine by air in a neutral weak alkaline solution. It has a wide range of uses, mainly in medicine, cosmetics, biochemical research, etc. This product has a detoxification effect and can also be used for acrylonitrile poisoning and aromatic poisoning. In cosmetics, it is mainly used in beauty water, perm solution, sunscreen, etc. Utilizing the special properties of cysteine ​​desulfurase in physiological functions, it can clean and remove toxic metals and semi-metals in the body of tattooers. It has an effective detoxification effect on a wide range of toxic substances, such as formaldehyde, acetaldehyde, chloroform, carbon tetrachloride, lead, cadmium, chloromethylmercury, lipid peroxide, PCB, pufferfish poison, alcohol, etc. It can maintain the activity of important sulfhydryl enzymes in the production of keratin in skin proteins, and supplement sulfur groups to maintain normal skin metabolism and regulate the bottom melanin produced by the pigment cells in the lowest layer of the epidermis. It is an ideal natural whitening cosmetic. It can also remove melanin in the skin, change the nature of the skin itself, and make the skin naturally white. It can also prevent inflammation. When allergies reduce sulfhydryl enzymes such as bile phosphatase, replenishing L-cysteine ​​can maintain the activity of sulfhydryl enzymes and improve skin symptoms of inflammation and allergies. Therefore, by utilizing the physiological function characteristics of cysteine ​​desulfurase, the metal and semi-metal poisons such as mercuric sulfide in red, chromium oxide in green, cobalt aluminate in blue, cadmium sulfide in yellow, and cadmium salts in brown in the body of tattooed people are detoxified, and then excreted from the body by using their own metabolic function. Using cysteine ​​desulfurase, in the process of tattoo cleaning, it can prevent the loss of skin keratin, supplement sulfur groups to maintain normal skin metabolism, and remove melanin in the skin. All of the above have been proven by clinical experiments.

[0029] (3) Oxide dismutase: Oxide dismutase is an enzyme related to copper, zinc and manganese. Superoxide dismutase (SOD) is also called peroxide dismutase. SOD belongs to metalloenzyme. According to the type of metal ions it binds, there are three types of this enzyme: copper- and zinc-containing superoxide dismutase, manganese-containing superoxide dismutase and iron-containing superoxide dismutase. All three types of SOD catalyze the dismutation of superoxide anion free radicals into hydrogen peroxide and oxygen. Therefore, using oxide dismutase, the copper ions in the green, zinc ions in the white, manganese ions in the purple and iron red in the body of the tattooed person are removed, which plays a role in protecting the skin and whitening. It can maximize the formation of non-toxic soluble complexes with copper ions, zinc ions, manganese ions and iron ions, and excrete them from the body through metabolic function.

[0030] (4) Catalase: Catalase (CAT) is an enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water. Hydrogen peroxide (H2O2) has strong oxidizing properties and can penetrate most cell membranes. Therefore, it has stronger cytotoxicity than superoxide anion free radicals (which cannot penetrate cell membranes). After penetrating the cell membrane, it can react with iron ions in the cell to generate hydroxyl free radicals. Catalase (CAT) is an important member of the antioxidant enzyme system. It is also called catalase, which is a conjugated enzyme with iron porphyrin as a cofactor. S0D enzyme generates hydrogen peroxide (H2O2) and oxygen (O2) after dismutation of free radicals. Hydrogen peroxide is still a substance with oxidant toxicity in the body. The role of catalase is to promote the decomposition of hydrogen peroxide into molecular oxygen and water, so that cells are free from the poisoning of H2O2. Therefore, catalase is used to catalyze the dismutation of three kinds of S0D anion free radicals into hydrogen peroxide during the tattoo removal dismutation reaction, so that it can be excreted from the body through metabolic function, preventing a large amount of hydrogen peroxide from damaging the cell membrane and causing harm to the body of the tattoo remover.

[0031] The functions and uses of penetrants are as follows:

[0032] (1) Laurocapram. So far, among all transdermal penetration enhancers, the most researched and applied one is Laurocapram (AZONE). Azone is a new type of highly effective penetration enhancer, which is characterized by having a good promoting effect on many hydrophilic and lipophilic synthetic drugs and natural drugs. As a penetrant, it is used in various creams and hair products in cosmetics, and is used as a penetration enhancer for applying external drugs and massage.

[0033] (2) Dimethyl isosorbide: abbreviated as DM1. Its functions are: enhancing the penetration and targeted delivery of active ingredients, reducing the concentration of active ingredients and their irritation to the skin. It improves the storage stability of the formula and is particularly suitable for easily hydrolyzed or transesterified formulas. It can be mixed with most organic solvents and non-ionic surfactants, and can be soluble in various types of products such as transparent gels. It can deliver water-soluble active ingredients to the skin without causing the product to crystallize.

[0034] (3) N-alkylbenzisothiazolone, its characteristics include: <1> , it has high purity, and a small amount is added when used, while the main drug penetrates the skin more, thereby improving the bioavailability of the drug. <2> It has no incompatibility with any component in the preparation, and it has a penetration-promoting effect when combined with a variety of Chinese and Western medicines. It is an amphiphilic penetration enhancer with a wide range of applications and has a penetration-promoting effect on both hydrophilic and hydrophobic drugs. <3> , it has no pharmacological effect itself, is eliminated quickly in the body, and has low bioavailability. <4> 2. The time it takes for the penetrated drug to reach its peak blood concentration in the body is short and the effect takes effect quickly.

[0035] (4) Cyclohexanehexol, its characteristics include: significant osmotic effect, which can improve the metabolism of dermal fibroblasts, protect keratinocytes from water loss due to hypertonic pressure, maintain the cell size / volume and water balance of keratinocytes under hypertonic conditions, and is a precursor of multiple metabolic pathways of skin cells.

[0036] (5) Tetrahydropiperidin can quickly help the functional ingredients reach the designated place, increase the fluidity of the lipid bilayer, open the tight junction proteins between cells, and reach other layers through the stratum corneum.

[0037] The functions and uses of auxiliary materials.

[0038] (1) Urea: moisturizing, softening the stratum corneum, promoting skin health, anti-inflammatory and anti-allergic, etc.

[0039] (2) Citric acid: It has a strong attraction to iron and copper ions, which prevents white cell membranes from turning black. It can speed up the body's metabolism, promote the renewal of the skin's stratum corneum, and make the skin shiny.

[0040] (3) Vitamin C: It has extremely strong antioxidant properties and can reduce pigments such as manganese, iron, and chromium into other substances.

[0041] (4) Tea polyphenols: Tea polyphenols have the ability to catalyze the degradation of dyes. Tea polyphenol silver ions have a significant catalytic effect on acidic dyes. They also have strong adsorption and antioxidant properties, and can degrade harmful substances, thereby stabilizing the color of the skin.

[0042] (5) Sodium gluconate: regulates the internal acid-base balance to restore the normal function of nerves. At the same time, it can promote the decrease of blood osmotic pressure, increase the excitability of nerves and muscles, and can make relevant drugs promote the recovery of nerve function, ensuring the recovery of injured nerves during tattooing and tattoo removal.

[0043] (6) Grape seed anthocyanin: It has a certain effect on skin whitening. It can help reduce melanin and has anti-inflammatory effects, which can reduce the occurrence of pigmentation caused by inflammation.

[0044] (7) Ethanol: It has good solubility and stability. As a carrier of decolorizing agent, it reduces the absorption capacity of pigment molecules by interacting with pigment molecules, thereby achieving the purpose of decolorization. It can also increase the permeability of cell membranes and cause color to fall off.

[0045] (8) Gelatin: It captures colors and impurities through its protein’s good adsorption properties. It also has the effect of moisturizing and protecting skin elasticity.

[0046] In a second aspect, the present invention provides a method for preparing a painless and efficient composite bio-enzyme tattoo remover, comprising the following steps:

[0047] Step 1, first add laccase, cysteine ​​desulfurase, oxide dismutase, and catalase into a reaction agitator respectively, and distilled water should be added to the reactor in advance, the amount of distilled water added is 30% of the total water, continue stirring, and stand for use;

[0048] Step 2, adding the penetrant into another stirred reactor pre-filled with distilled water, the amount of water added is 30% of the total amount of water used, and after continuing to stir, the reactor is allowed to stand for use;

[0049] Step 3, add the remaining 40% of the distilled water into the reactor, then add urea and stir to dilute it evenly, then add anhydrous citric acid, sodium gluconate and tea polyphenols into the reactor in that order, with a time interval of 15 minutes between each addition of materials, and stir continuously until all are diluted and ready for use; since the application of tea polyphenols requires an acidic environment, citric acid needs to be added first to create an acidic environment.

[0050] Step 4, adding the composite bio-enzyme preparation in step 1, the composite penetrant in step 2 and the auxiliary agent in step 3 into a stirred reactor, stirring for reaction, leaving it to stand, sterilizing it and filling it for use.

[0051] It also includes step 5, packaging. The packaging must be in brown glass bottles and must be stored in a light-proof place with a temperature controlled within the range of 10-25.

[0052] In a third aspect, the present invention provides a painless and highly effective composite bio-enzyme tattoo remover for use in tattoo removal.

[0053] Compared with the prior art, the present invention has the following beneficial effects:

[0054] (1) The present invention provides a painless and efficient composite bio-enzyme tattoo remover, comprising a composite bio-enzyme, a penetrant and an adjuvant; in the composite bio-enzyme, various single enzyme genes have the characteristics of being most suitable for enzyme activity reaction temperature, good thermal stability and fast reaction speed. The proportions of various enzyme components in the composite enzyme preparation can be compounded in an ideal ratio to maximize the synergistic promotion effect of the enzyme preparation, and effectively improve the inhibitory reaction caused by the deficiency of some single enzyme preparations in natural enzyme preparations.

[0055] (2) The composite biological enzyme in the tattoo remover provided by the present invention has clear components and known action sites, which can effectively prevent the generation of by-products and the occurrence of unexpected allergic reactions, and effectively prevent contamination by miscellaneous bacteria.

[0056] (3) Develop painless and highly effective composite bio-enzyme tattoo removers that generally have good pushability and a long enzyme half-life, which can significantly save the amount of enzyme preparations used.

[0057] (4) This preparation is colorless, odorless, non-toxic, has no side effects, has a neutral pH value, and is composed of pure natural organic enzymes, penetrants, and additives, all of which are of medical and cosmetic grade and are safe and reliable.

[0058] (5) The preparation provided by the present invention is used to remove tattoos without pain, swelling, allergy, adverse reactions or sequelae.

[0059] (6) The preparation method is simple, easy to store and transport, and simple to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0061] Figure 1 This is a comparison of the tattoo pigment removal effects of the composition of Example 1 in the test example; the left side is before removal, and the right side is after removal.

[0062] Figure 2 This is a comparison of the tattoo pigment removal effects of the composition of Example 2 in the test example; the left side is before removal, and the right side is after removal. DETAILED DESCRIPTION

[0063] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0064] Example 1, a preferred method for preparing formulation No. 1.

[0065] (1) First, add 1-1.5 parts of laccase, 1-2.5 parts of cysteine ​​desulfurase, 1-3 parts of oxide dismutase, and 1-3 parts of catalase into the reaction agitator (distilled water should be added to the reactor in advance, and the amount of distilled water added is 30% of the total water), continue stirring for 60 minutes, and let it stand for 2 hours for use.

[0066] (2) Add 1-2 parts of penetrant azone, 1-3 parts of dimethyl isosorbide, and 1-2 parts of thiothiazone into a stirred reactor pre-filled with distilled water (the amount of water added is 30% of the total amount of water), continue stirring for 60 minutes, and then stand for 2 hours for use.

[0067] (3) Auxiliary materials: First, add distilled water (40% of the total amount of water) into the reactor as required, then add 1-5.0 parts of urea and stir to dilute evenly, then add anhydrous citric acid: 1-2.0 parts; sodium gluconate: 1-3.0 parts; tea polyphenols: 0.5-2.0 parts into the reactor in the following order (the time interval between each addition of the medicine is required to be 15 minutes), and stir continuously until all diluted and ready for use.

[0068] (4) Add the composite bio-enzyme preparation, the composite penetrant and its auxiliary materials into a stirred reactor in a ratio of (1:1:2), stir and react for 90 minutes, let it stand for 24 hours, inspect and sterilize it, and then fill it for use.

[0069] (5) The product must be packaged in brown glass bottles and stored in a dark place at a temperature between 10-25°C.

[0070] Example 2, the preparation method of the preferred formulation No. 2

[0071] (1) First, add 1-3 parts of laccase, 1-2 parts of cysteine ​​desulfurase, 1-2.5 parts of oxide dismutase, and 1-2.5 parts of catalase to a stirred reactor pre-added with quantum activated small molecule water (the amount of water added is 30% of the total water amount), stir for 60 minutes, and then stand for 2 hours for use. (2) Add the penetrant, 1-3.5 parts of azone, 1-2.5 parts of cyclohexanehexol, and 1-2 parts of tetrahydropiperazine to the pre-treated activated water reactor in the required proportion and order (the amount of water added is 30% of the total amount of water added), stir and dilute for use. Auxiliary material urea: 1-5.0 parts. Add them to the stirred reactor with quantum activated water added in advance (the amount of water added is 40% of the total amount of water added), stir for 60 minutes, then add grape seed anthocyanin: 0.5-3.0 parts, vitamin C: 1-1.5 parts, ethanol: 1-5.0 parts according to the characteristics of the medicine, and let it stand for 2 hours before use. (3) Add the compounded bioenzyme preparation and the compounded permeabilizer and its auxiliary materials to the stirred reactor in a ratio of (1:1:3), stir for 90 minutes, let it stand for 24 hours before use, and after testing, sterilization and disinfection, fill it for use. (4) Brown glass bottles must be used for filling, stored in a dark and dry place, and the temperature is controlled at 10-25 degrees.

[0072] Test example

[0073] (I) How to use:

[0074] (1) Before use, check whether the biological enzyme preparation has deteriorated. Then gently shake it by hand to make it uniform.

[0075] (2) Before use, the tattooed area must be cleaned with warm water. After the skin is dried, use a cotton ball to apply the bio-enzyme preparation or spray the bio-enzyme preparation on the tattooed area. It is required to scrub the tattooed area once in the morning and evening. It is best to gently rub the tattooed area with your hands each time you scrub it to facilitate removal. However, the interval between each treatment should be 12 hours to provide the skin with a repair period.

[0076] (3) During use, if any adverse reactions such as allergies, skin redness, swelling, itching, etc. occur, wash it immediately with clean water (do not use alcohol) and stop using it as soon as possible.

[0077] (2) Notes

[0078] (1) Users must not drink alcohol, have a cold or fever before use, take a shower immediately after processing, or expose to the sun after processing. (2) This product should be sealed and stored at room temperature (10-25℃) away from light. It should not be transported or stored together with strong oxidants, reducing agents, strong alkalis, or strong acids. (3) This product is valid for one year.

[0079] (III) Results

[0080] The experiment was conducted by taking animal skin from a body. Different pigments were first inserted into the dermis of the skin using a tattoo gun, and then the tattoos of different colors were removed using the composite bio-enzyme tattoo remover provided in Example 1. The results are shown in FIG. Figure 1 As shown, the red and green tattoo pigments are completely removed, the black and blue are basically removed, and the yellow tattoo pigment is mostly removed. This product has a good tattoo removal effect.

[0081] The tattoo removal was performed using the composite bio-enzyme tattoo remover provided in Example 2. Figure 2 As shown, yellow, green, purple, and black tattoo pigments were completely removed, and red tattoo pigments were mostly removed. This product has a good tattoo removal effect.

[0082] The biggest advantage of the product of this application is that it has no irritation, no swelling, no allergy, no adverse reaction, and no sequelae. It can be removed quickly and thoroughly without leaving scars. It is easy to use, suitable for storage, low cost, cheap, suitable for all kinds of people, and easy to promote.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A painless and efficient composite bio-enzyme tattoo remover, characterized in that: The invention comprises a composite biological enzyme, wherein the composite biological enzyme comprises the following components: Laccase: 1-3 parts; cysteine ​​desulfurase: 1-2.5 parts; oxide dismutase: 1-3 parts; one or more of catalase 1-3 parts.

2. The painless and highly effective composite bio-enzyme tattoo remover according to claim 1, characterized in that: Laccase is 1-2.5 parts; or cysteine ​​desulfurase: 1-2 parts; or oxide dismutase: 1-2.5 parts; Or 1-2.5 parts of catalase.

3. The painless and highly effective composite bio-enzyme tattoo remover according to claim 1, characterized in that: The tattoo remover further comprises a penetrant, which comprises the following components: One or more of azone: 1-3.5 parts; dimethyl isosorbide: 1-3 parts; N-n-alkylbenzisothiazine or oxazolidinone: 1-2 parts; cyclohexanehexol: 1-2.5 parts.

4. The painless and efficient composite bio-enzyme tattoo remover according to claim 3, characterized in that: The azone is lauryl azone, and the tattoo remover contains 1-2.5 parts of lauryl azone.

5. The painless and highly effective composite bio-enzyme tattoo remover according to claim 3, characterized in that: The tattoo remover comprises 1.5 parts of dimethyl isosorbide and 1-1.5 parts of N-n-alkyl benzisothiazone or oxazolidinone.

6. The painless and highly effective composite bio-enzyme tattoo remover according to claim 1, characterized in that: The tattoo remover further comprises an auxiliary agent, which comprises the following components: one or more of gelatin, urea, citric acid, sodium gluconate, ethanol, tea polyphenols, grape seed anthocyanin, and vitamin C.

7. The painless and highly effective composite bio-enzyme tattoo remover according to claim 1, characterized in that: The auxiliary agent includes gelatin: 1-5 parts; anhydrous citric acid: 1-2 parts; sodium gluconate: 1-3 parts; ethanol: 1-5 parts; Tea polyphenols: 0.5-2 parts; grape seed anthocyanins: 0.5-3 parts; vitamin C: 1-1.5 parts; Urea: 1-5 parts.

8. The painless and highly effective composite bio-enzyme tattoo remover according to claim 1, characterized in that: It also contains 1-80 parts of distilled water.

9. A method for preparing a painless and highly effective composite bio-enzyme tattoo remover, characterized in that: The following steps are included: Step 1, first add laccase, cysteine ​​desulfurase, oxide dismutase, and catalase into a reaction agitator respectively, and distilled water should be added to the reactor in advance, the amount of distilled water added is 30% of the total water, continue stirring, and stand for use; Step 2, adding the penetrant into another stirred reactor pre-filled with distilled water, the amount of water added is 30% of the total amount of water used, and after continuing to stir, the reactor is allowed to stand for use; Step 3, add the remaining 40% of the total amount of water into the reactor, then add urea and stir to dilute evenly, then add anhydrous citric acid, sodium gluconate and tea polyphenols into the reactor in the order of first and last addition, requiring that the time interval between each addition of materials is 15 minutes, and stirring is continued until all are diluted and set aside; Step 4, adding the composite bio-enzyme preparation in step 1, the composite penetrant in step 2 and the auxiliary agent in step 3 into a stirred reactor, stirring for reaction, leaving it to stand, sterilizing it and filling it for use.

10. Use of the painless and highly effective composite bio-enzyme tattoo remover according to any one of claims 1 to 8 in tattoo removal.

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