Application of interleukin 12P70 in the detection of rheumatoid arthritis

Abstract

The present invention discloses an application of interleukin 12P70 in rheumatoid arthritis detection, and belongs to the field of molecular biology of rheumatoid arthritis. Interleukin 12P70 is used as a detection index for rheumatoid arthritis for the diagnosis of rheumatoid arthritis. Therefore, the preparation for detecting interleukin 12P70 can be applied to the preparation of rheumatoid arthritis diagnostic preparations. The results showed that the expression level of IL-12P70 in the RA group was higher than that in the normal group. Through the correlation analysis of IL-12P70 with the RA patient's disease severity indicators ESR, RF, anti-CCP antibodies, DS28 disease activity score, it was found that IL-12P70 in RA patients was positively correlated with anti-CCP antibodies, ESR and DS28 disease activity score. Therefore, IL-12P70 plays an important role in the pathological mechanism of rheumatoid arthritis.

Description

Technical Field

[0001] The invention belongs to the field of rheumatoid arthritis molecular biology, and specifically relates to an application of interleukin 12P70 in rheumatoid arthritis detection. Background Art

[0002] Rheumatoid arthritis is an autoimmune disease with synovial inflammation, cartilage and bone destruction as its main pathological features. However, there is currently a lack of effective early diagnosis and treatment for the disease. Misdiagnosis and mistreatment can lead to severe disability and ultimately early death.

[0003] Rheumatoid arthritis can cause morning stiffness, swelling, pain, deformity, and dislocation of joints throughout the body, especially the proximal small joints. It can also affect the lungs, kidneys, nervous system, gastrointestinal system, etc. The assessment of systemic lesions of rheumatoid arthritis is particularly important. Therefore, early diagnosis of rheumatoid arthritis is conducive to delaying the course of rheumatoid arthritis and improving the prognosis of patients with rheumatoid arthritis. Summary of the invention

[0004] In view of the current shortcomings of rheumatoid arthritis such as the lack of diagnostic markers, the present invention provides an application of interleukin 12P70 in rheumatoid arthritis detection, which is used to solve the problems such as the lack of diagnostic evaluation in the prior art.

[0005] In order to achieve this object, the present invention proposes an application of interleukin 12P70 in the detection of rheumatoid arthritis. Interleukin 12P70 is used as a detection index for rheumatoid arthritis for the diagnosis of rheumatoid arthritis. Therefore, the preparation for detecting interleukin 12P70 can be used in the preparation of a diagnostic preparation for rheumatoid arthritis. Of course, interleukin 12P70 can also be used for the auxiliary diagnosis of rheumatoid arthritis.

[0006] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0007] 1. Through observation, it was found that the ROC curve analysis of IL-12P70 in the RA group and the normal group showed that the (total) sensitivity and specificity of the RA group were more significantly improved. By comparing the expression levels of IL-12P70 in the RA group and the normal group, the difference in the expression of IL-12P70 in the RA group and the normal group was observed. The results showed that the expression level of IL-12P70 in the RA group was higher than that in the normal group. Through the correlation analysis of IL-12P70 with the disease severity indicators of RA patients, ESR, RF, anti-CCP antibodies, and DS28 disease activity score, it was found that IL-12P70 was positively correlated with anti-CCP antibodies in RA patients (r=0.285, P=0.027). IL-12P70 was positively correlated with ESR (r=0.361, P<0.01), and IL-12P70 was positively correlated with DS28 disease activity score (r=0.369, P<0.01). Therefore, IL-12P70 plays an important role in the pathogenesis of rheumatoid arthritis.

[0008] 2. Experiments have confirmed that IL-12P70 plays an important role in rheumatoid arthritis and its systemic lesions. The assessment of the severity of rheumatoid arthritis can not only predict the prognosis of patients with rheumatoid arthritis, but also guide the clinical medication of rheumatoid arthritis, and has far-reaching significance for the evaluation of the results of immune function regulation in patients with rheumatoid arthritis. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 is the ROC curve.

[0010] Figure 2 Comparison of inflammatory IL-12P70 expression levels.

[0011] Figure 3 The correlation between the disease severity indicators ESR, RF, CCP, DS28 disease activity score and IL-12P70 was analyzed. DETAILED DESCRIPTION

[0012] The present invention is further described below in conjunction with specific embodiments, which are only used to explain the relevant principles and steps of the present invention and cannot be understood as limiting the present invention. It can be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and purpose of the present invention.

[0013] Experimental procedures

[0014] 1Immune inflammation related indicators

[0015] 3 mL of fasting forearm venous blood was collected from the subjects using an erythrocyte sedimentation tube (black tube), and the erythrocyte sedimentation rate (ESR) was detected using an automatic erythrocyte sedimentation analyzer; rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibody (Anti-CCP-Ab): 5 mL of forearm venous blood was collected from the subjects using a biochemical tube (yellow tube), and after coagulation at 3-5°C, it was placed in a centrifuge at 4000r / min for 15 minutes, and the serum was separated and stored in a -80°C refrigerator. After all the specimens were collected, they were detected by an automatic biochemical analyzer. The DS28 score of RA patients was calculated using the software method.

[0016] 2IL-12P70 detection

[0017] IL-12P70 was detected by flow cytometry. Methods: 1 mL of venous whole blood was collected using a heparin anticoagulant tube (green tube). After separating the serum, the specimen to be tested was diluted with the specimen diluent, and then the sample was added, incubated, and the luminescent substrate was added and mixed thoroughly according to the operating steps, and the reaction was carried out in a 4°C refrigerator for 30 minutes. Centrifuged at 1500r / min for 5 minutes, and the supernatant was removed. The cells were resuspended in PBS and detected by flow cytometry. The results were analyzed using Flow Jo 7.6.1 software.

[0018] Experimental results:

[0019] (1) The ROC curve results showed that the area under the ROC curve of IL-12P70 in the RA group was 0.832 (P < 0.01) compared with the normal group ( Figure 1 and shown in Table 1).

[0020] Table 1

[0021]

[0022] (2) By comparing the expression levels of inflammatory IL-12P70 in the RA group and the normal group, the difference in the expression of inflammatory IL-12P70 in the RA group and the normal group was observed. The results showed that the expression level of IL-12P70 in the RA group was higher than that in the normal group (P<0.01 or P<0.05) ( Figure 2 shown).

[0023] (3) The correlation analysis between RA patients' disease severity indicators ESR, RF, CCP, DS28 disease activity score and IL-12P70 showed that IL-12P70 was positively correlated with anti-CCP antibodies in RA patients (r=0.285, P=0.027). IL-12P70 was positively correlated with ESR (r=0.361, P<0.01). IL-12P70 was positively correlated with DS28 disease activity score (r=0.369, P<0.01) ( Figure 3 shown).

[0024] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. An application of interleukin 12P70 in the detection of rheumatoid arthritis, characterized in that: Interleukin 12P70 is used as a detection indicator for rheumatoid arthritis and for the diagnosis of rheumatoid arthritis.

2. An application of interleukin 12P70 in the detection of rheumatoid arthritis, characterized in that: Application of preparations for detecting interleukin 12P70 in the preparation of diagnostic preparations for rheumatoid arthritis.

3. The use according to claim 1 or 2, characterized in that: There were differences in the expression of IL-12P70 between the RA group and the normal group: the expression level of IL-12P70 in the RA group was higher than that in the normal group; through the correlation analysis of RA patients' disease severity indicators ESR, RF, anti-CCP antibodies, DS28 disease activity score and IL-12P70, it was found that IL-12P70 in RA patients was positively correlated with anti-CCP antibodies, IL-12P70 was positively correlated with ESR, and IL-12P70 was positively correlated with DS28 disease activity score.

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