Immunoassay microwell plate sample adding auxiliary device

Abstract

The invention discloses an immunoassay microwell plate sample adding auxiliary device, and belongs to the field of microwell plate sample adding. Comprising a placing table, clamping plates are fixedly connected to the left side of the upper surface of the placing table in a front-back symmetry mode, two telescopic air pipes are fixedly connected to the positions, located on the left sides of the clamping plates, of the upper surface of the placing table, and an inflation box I is fixedly connected to the front surface of the placing table; and first ventilation hoses are fixedly connected between the first inflation box and the two telescopic air pipes correspondingly. When the device is used, a plurality of microwell plate bodies are stacked and placed between two clamping plates, then a motor is started to drive a cam to rotate, under linkage of a traction rod, a first piston plate can be pulled to repeatedly move left and right, and then a first inflation box can be utilized to repeatedly inflate and exhaust air into a telescopic air pipe; then the pushing strip is controlled to repeatedly move left and right, so that the microwell plate bodies can be pushed one by one to move to the right for sample adding treatment.

Description

Technical Field

[0001] The invention relates to the field of microplate sample addition, in particular to an immunoassay microplate sample addition auxiliary device. Background Art

[0002] Medical testing is a science that conducts microbiological, immunological, biochemical, genetic, hematological, biophysical, cytological and other tests on materials taken from the human body, thereby providing information for the prevention, diagnosis, treatment of human diseases and the assessment of human health.

[0003] Patent: CN220835666U discloses an auxiliary device for sample loading on a microplate of an immunoassay, including a base, a positioning groove is provided on the top of the base, a pre-tightening support mechanism is fixedly connected to the front right side of the top of the base, a sample loading mechanism is connected to the top of the pre-tightening support mechanism, a microplate positioning mechanism is arranged on the top of the base, the pre-tightening support mechanism includes a slide bar, a limit block, a spring and a connecting block, the slide bar is fixedly connected to the front right side of the top of the base, the limit block is fixedly connected to the top of the slide bar, the spring is sleeved on the outside of the slide bar, and the connecting block is slidably connected to the outer ring of the slide bar. The auxiliary device for sample loading on a microplate of an immunoassay can drip liquid into all the wells of the microplate at one time because the sample loading mechanism has multiple dropper heads distributed in a matrix, and the sample loading process can be made more accurate and stable by positioning the pre-tightening support mechanism, thereby improving the overall efficiency of the detection;

[0004] The above technology is not convenient for sequentially dripping liquids on multiple microplates, and is not convenient for automatic dripping and inconvenient to operate, and needs to be improved. For this reason, we propose an immunoassay microplate loading auxiliary device. Summary of the invention

[0005] Purpose of the invention: The purpose of the present invention is to provide a device that is convenient for dripping liquid on multiple microplates in sequence; another purpose of the present invention is to provide a device that is convenient for automatic dripping and easy to operate.

[0006] Technical solution: An immunoassay microplate loading auxiliary device comprises a placing table, a clamping plate is symmetrically fixedly connected to the left side of the upper surface of the placing table, two telescopic air tubes are fixedly connected to the upper surface of the placing table located on the left side of the clamping plate, an inflation box 1 is fixedly connected to the front surface of the placing table, a ventilation hose 1 is fixedly connected between the inflation box 1 and the two telescopic air tubes, a piston plate 1 is slidably connected to the inside of the inflation box 1, a traction rod is fixedly connected to the right side of the piston plate 1, the right end of the traction rod passes through the right side of the inflation box 1 and is slidably connected to the inflation box 1, an L-shaped mounting plate is fixedly connected to the front surface of the placing table, a motor is fixedly connected to the rear surface of the L-shaped mounting plate, a cam is fixedly connected to the rear end of the output shaft of the motor, the cam is movably connected to the traction rod, and a push bar is fixedly connected to the right ends of the two telescopic air tubes;

[0007] A mounting frame is fixedly connected to the right side of the upper surface of the placing table in a front-to-back symmetrical manner, a liquid storage box is fixedly connected between the two mounting frames, and a plurality of dripping pipes are fixedly connected to the lower side of the liquid storage box.

[0008] Furthermore, a transverse sliding groove is provided on the upper surface of the placement table, and L-shaped sliding grooves are provided on opposite sides of the two clamping plates.

[0009] Furthermore, a slider is slidably connected inside the L-shaped slide groove, a micro-plate body is fixedly connected between the two sliders facing each other, a limiting slide bar is fixedly connected to the lower surface of the micro-plate body, and the limiting slide bar is adapted to the transverse slide groove.

[0010] Furthermore, a wedge-shaped surface is integrally formed on the right side of the microporous plate body.

[0011] Furthermore, a liquid injection pipe is integrally formed at the rear of the upper surface of the liquid storage tank, and a pipe head is threadedly connected to the top of the liquid injection pipe.

[0012] Furthermore, the front surface of the liquid storage tank is fixedly connected to an air filling box 2, a ventilation hose 2 is fixedly connected between the air filling box 2 and the liquid storage tank, a piston plate 2 is slidably connected inside the air filling box 2, and a spring 1 is fixedly connected between the piston plate 2 and the air filling box 2.

[0013] Furthermore, the front surface of the mounting frame located in front of the upper surface of the placing table is rotatably connected to an extrusion rod via a rotating shaft, an extrusion head is integrally formed at the top of the extrusion rod, the outer side wall of the extrusion head is in contact with the lower surface of the piston plate 2, and a plurality of springs 2 are fixedly connected to the right side of the extrusion rod, and the right ends of the plurality of springs 2 are fixedly connected to a fixing block together with the front surface of the placing table.

[0014] Furthermore, a push rod is provided on the front surface of the placement table, a linkage rod is fixedly connected between the push rod and the push bar, and a ball head is fixedly connected to the right end of the push rod.

[0015] Furthermore, a connecting groove is formed on the front surface of the cam, and a connecting column is fixedly connected to the rear surface of the traction rod inside the connecting groove.

[0016] Beneficial effect: When the device is used, multiple microplate bodies are stacked and placed between two clamping plates, and then the motor is started to drive the cam to rotate. Under the linkage of the traction rod, the piston plate can be pulled to move left and right repeatedly, and then the air box can be used to repeatedly inflate and exhaust air into the telescopic air pipe, and then the push bar can be controlled to move left and right repeatedly, so that the microplate bodies can be pushed one by one to move to the right for sample addition processing;

[0017] The L-shaped slide groove can be used to stack multiple microplate bodies between the two clamping plates, and when pushed, they can slide out from between the two clamping plates. The limit slide bar and the transverse slide groove can prevent the microplate body from deviating when sliding from left to right, which is convenient for subsequent alignment and sample addition.

[0018] When the push bar moves to the right and pushes the microplate body to the right to the bottom of the liquid storage tank for adding liquid, the ball head will move to the right together with the linkage rod. When the ball head contacts the squeezing rod and continues to move to the right, the squeezing rod will be activated by the toggle. At this time, the squeezing head will not squeeze the piston plate 2. When the microplate body pushes the position, as the push bar resets to the left, the ball head will move to the left together, contact the squeezing rod at this time, and push the squeezing rod to rotate. The squeezing head will squeeze the piston plate 2 to rise, thereby squeezing the liquid out of the dropper tube to achieve automatic sample addition. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 It is a cross-sectional structural schematic diagram of the inflatable box 1, the cam and the traction rod of the present invention;

[0021] Figure 3 It is a schematic diagram of the cross-sectional connection structure of the second inflation box and the liquid outlet box of the present invention;

[0022] Figure 4 It is a schematic diagram of the connection structure of the extrusion rod and the fixing block of the present invention;

[0023] Figure 5 It is a structural schematic diagram of the microporous plate body of the present invention.

[0024] In the figure: 1. placing table; 2. clamping plate; 3. telescopic air pipe; 4. inflation box one; 5. ventilation hose one; 6. piston plate one; 7. traction rod; 8. L-shaped mounting plate; 9. motor; 10. cam; 11. push bar; 12. mounting frame; 13. liquid storage tank; 14. drip tube; 15. horizontal slide groove; 16. L-shaped slide groove; 17. slider; 18. microplate body; 19. limit slide bar; 20. wedge surface; 21. injection tube; 22. pipe head; 23. inflation box two; 24. ventilation hose two; 25. piston plate two; 26. spring one; 27. extrusion rod; 28. extrusion head; 29. ​​spring two; 30. fixing block; 31. push rod; 32. linkage rod; 33. ball head; 34. connecting groove; 35. connecting column. DETAILED DESCRIPTION

[0025] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] Example 1

[0027] like Figure 1 and Figure 2 As shown, an immunoassay microplate loading auxiliary device is provided, comprising a placing table 1, a clamping plate 2 is symmetrically fixedly connected to the left side of the upper surface of the placing table 1, two telescopic air tubes 3 are fixedly connected to the upper surface of the placing table 1 located on the left side of the clamping plate 2, an inflatable box 4 is fixedly connected to the front surface of the placing table 1, and a ventilation hose 5 is fixedly connected between the inflatable box 4 and the two telescopic air tubes 3 respectively;

[0028] The interior of the inflatable box 4 is slidably connected with a piston plate 6, and a traction rod 7 is fixedly connected to the right side of the piston plate 6. The right end of the traction rod 7 penetrates to the right side of the inflatable box 4 and is slidably connected to the inflatable box 4. The front surface of the placement table 1 is fixedly connected with an L-shaped mounting plate 8, and the rear surface of the L-shaped mounting plate 8 is fixedly connected with a motor 9. The rear end of the output shaft of the motor 9 is fixedly connected with a cam 10, and the cam 10 is movably connected to the traction rod 7. The right ends of the two telescopic air pipes 3 are fixedly connected with a push bar 11;

[0029] A connecting groove 34 is formed on the front surface of the cam 10, and a connecting column 35 is fixedly connected to the rear surface of the traction rod 7 at the inner side of the connecting groove 34;

[0030] When the device is in use, multiple microplate bodies 18 are stacked and placed between two clamping plates 2, and then the motor 9 is started to drive the cam 10 to rotate. Under the linkage of the traction rod 7, the piston plate 6 can be pulled to move left and right repeatedly, and then the inflation box 4 can be used to repeatedly inflate and exhaust air into the telescopic air tube 3, and then the pushing bar 11 is controlled to move left and right repeatedly, so that the microplate bodies 18 can be pushed one by one to move to the right for sample addition processing.

[0031] like Figure 1 and Figure 5 As shown, a transverse slide groove 15 is provided on the upper surface of the placement table 1, and L-shaped slide grooves 16 are provided on opposite sides of the two clamping plates 2;

[0032] A slider 17 is slidably connected inside the L-shaped slide groove 16, and a microplate body 18 is fixedly connected between the two sliders 17 facing each other. A limit slide bar 19 is fixedly connected to the lower surface of the microplate body 18, and the limit slide bar 19 is adapted to the horizontal slide groove 15;

[0033] A wedge-shaped surface 20 is integrally formed on the right side of the microplate body 18;

[0034] By means of the L-shaped slide groove 16, a plurality of microplate bodies 18 can be stacked between the two clamping plates 2, and can slide out from between the two clamping plates 2 when pushed. By means of the limit slide bar 19 and the transverse slide groove 15, the microplate body 18 will not deviate when sliding from left to right, which is convenient for subsequent alignment and sample loading.

[0035] like Figure 3 As shown, a mounting frame 12 is symmetrically fixedly connected to the upper surface of the placement table 1 on the right side, and a liquid storage tank 13 is fixedly connected between the two mounting frames 12. A plurality of dripping pipes 14 are fixedly connected to the lower side of the liquid storage tank 13.

[0036] A liquid injection pipe 21 is integrally formed at the rear of the upper surface of the liquid storage box 13, and a pipe head 22 is threadedly connected to the top of the liquid injection pipe 21;

[0037] The front surface of the liquid storage tank 13 is fixedly connected with an air box 23, a ventilation hose 24 is fixedly connected between the air box 23 and the liquid storage tank 13, a piston plate 25 is slidably connected inside the air box 23, and a spring 26 is fixedly connected between the piston plate 25 and the air box 23;

[0038] The injection tube 21 is convenient for adding liquid into the liquid storage box 13. By squeezing the piston plate 25 to inflate the liquid storage box 13, the internal liquid can be squeezed out through the multiple dropper tubes 14, thereby realizing the sample addition process to the microplate body 18.

[0039] like Figure 1 and Figure 4 As shown, the front surface of the mounting frame 12 located in front of the upper surface of the placing table 1 is rotatably connected to an extrusion rod 27 through a rotating shaft, and an extrusion head 28 is integrally formed at the top of the extrusion rod 27. The outer wall of the extrusion head 28 contacts the lower surface of the piston plate 25, and a plurality of springs 29 are fixedly connected to the right side of the extrusion rod 27. The right ends of the plurality of springs 29 and the front surface of the placing table 1 are fixedly connected to a fixing block 30;

[0040] A push rod 31 is provided on the front surface of the placement table 1, a linkage rod 32 is fixedly connected between the push rod 31 and the push bar 11, and a ball head 33 is fixedly connected to the right end of the push rod 31;

[0041] When the push bar 11 moves to the right and pushes the microplate body 18 to the right to the bottom of the liquid storage tank 13 for adding liquid, the ball head 33 will move to the right together under the linkage of the linkage rod 32. When the ball head 33 contacts the squeezing rod 27 and continues to move to the right, the squeezing rod 27 will be activated. At this time, the squeezing head 28 will not squeeze the piston plate 25. When the microplate body 18 finishes pushing the position, as the push bar 11 resets to the left, the ball head 33 will move to the left together, contact the squeezing rod 27, and push the squeezing rod 27 to rotate. The squeezing head 28 will squeeze the piston plate 25 to rise, thereby squeezing the liquid out of the dropper 14 to achieve an automatic sample addition effect.

[0042] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An immunoassay microplate sample loading auxiliary device, comprising a placement table (1), characterized in that: A clamping plate (2) is symmetrically fixedly connected to the left side of the upper surface of the placing platform (1); two telescopic air tubes (3) are fixedly connected to the upper surface of the placing platform (1) located on the left side of the clamping plate (2); an air filling box (4) is fixedly connected to the front surface of the placing platform (1); a ventilation hose (5) is fixedly connected between the air filling box (4) and the two telescopic air tubes (3); a piston plate (6) is slidably connected inside the air filling box (4); a traction member (6) is fixedly connected to the right side of the piston plate (6). A guide rod (7), the right end of the traction rod (7) passes through the right side of the inflation box (4) and is slidably connected to the inflation box (4); the front surface of the placement table (1) is fixedly connected to an L-shaped mounting plate (8); the rear surface of the L-shaped mounting plate (8) is fixedly connected to a motor (9); the rear end of the output shaft of the motor (9) is fixedly connected to a cam (10); the cam (10) is movably connected to the traction rod (7); the right ends of the two telescopic air tubes (3) are fixedly connected to a push bar (11); A mounting frame (12) is symmetrically fixedly connected to the right side of the upper surface of the placement table (1) in a front-to-back manner, a liquid storage box (13) is fixedly connected between the two mounting frames (12), and a plurality of dripping tubes (14) are fixedly connected to the bottom of the liquid storage box (13).

2. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: A transverse sliding groove (15) is provided on the upper surface of the placement platform (1), and L-shaped sliding grooves (16) are provided on opposite sides of the two clamping plates (2).

3. The immunoassay microplate sample loading auxiliary device according to claim 2, characterized in that: The L-shaped slide groove (16) is internally slidably connected with a slider (17), and a microplate body (18) is fixedly connected between two sliders (17) facing each other in the front and rear directions. A limit slide bar (19) is fixedly connected to the lower surface of the microplate body (18), and the limit slide bar (19) is adapted to the transverse slide groove (15).

4. The immunoassay microplate sample loading auxiliary device according to claim 3, characterized in that: A wedge-shaped surface (20) is integrally formed on the right side of the microporous plate body (18).

5. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: A liquid injection pipe (21) is integrally formed at the rear of the upper surface of the liquid storage box (13), and a pipe head (22) is threadedly connected to the top of the liquid injection pipe (21).

6. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: The front surface of the liquid storage tank (13) is fixedly connected to an air charging box 2 (23), a ventilation hose 2 (24) is fixedly connected between the air charging box 2 (23) and the liquid storage tank (13), a piston plate 2 (25) is slidably connected inside the air charging box 2 (23), and a spring 1 (26) is fixedly connected between the piston plate 2 (25) and the air charging box 2 (23).

7. The immunoassay microplate sample loading auxiliary device according to claim 6, characterized in that: The front surface of the mounting frame (12) located in front of the upper surface of the placement table (1) is rotatably connected to an extrusion rod (27) via a rotating shaft, and an extrusion head (28) is integrally formed at the top end of the extrusion rod (27). The outer wall of the extrusion head (28) contacts the lower surface of the piston plate 2 (25). A plurality of springs 2 (29) are fixedly connected to the right side of the extrusion rod (27), and the right ends of the plurality of springs 2 (29) are fixedly connected to a fixing block (30) together with the front surface of the placement table (1).

8. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: A pushing rod (31) is provided on the front surface of the placement table (1), a linkage rod (32) is fixedly connected between the pushing rod (31) and the pushing bar (11), and a ball head (33) is fixedly connected to the right end of the pushing rod (31).

9. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: The front surface of the cam (10) is provided with a connection groove (34), and the rear surface of the traction rod (7) is located inside the connection groove (34) and is fixedly connected with a connection column (35).

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