Composition with moisturizing and anti-aging effects and application of composition in cosmetics

Abstract

The invention discloses a composition with moisturizing and anti-aging effects and application of the composition in cosmetics, the moisturizing and anti-aging composition comprises retinyl sodium hyaluronate and a radix semiaquilegiae extract, and tests prove that the retinyl sodium hyaluronate and the radix semiaquilegiae extract have a synergistic effect, so that the moisturizing and anti-aging effects are achieved. The compound has a remarkable effect of promoting expression of an X VII type collagen gene and generation of AQP3 protein (aquaporin 3), can be used in cosmetics to improve the problems of dry skin and aging, and is good in stability, safe and non-irritant.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a composition with moisturizing and anti-aging effects and an application thereof in cosmetics. Background Art

[0002] In human tissues, collagen accounts for 70% to 80% of the dry weight. Type XVII collagen, as a transmembrane glycoprotein, is an important component of epidermal stem cell hemidesmosomes. It is mainly located in epidermal stem cells at the junction of the epidermis and dermis. Its function is to make the epidermis and dermis tightly connected through the basement membrane zone to promote epidermal replacement and stabilize the basement membrane. Type XVII collagen can effectively prevent the separation of the epidermis and dermis, avoid problems such as blisters and sagging of the skin, and maintain the stability and integrity of the overall structure of the skin. With age, the content of type XVII collagen in the skin will gradually decrease, resulting in a decrease in skin elasticity and toughness. Increasing the content of type XVII collagen in the skin can effectively delay the aging process of the skin.

[0003] Sodium retinoyl hyaluronate is an ester of all-trans retinoic acid and 13-cis retinoic acid with sodium hyaluronate. This structural formula is recorded in the invention patent (application number: 202411907798.8). All-trans retinoic acid, also known as retinoic acid, has the effects of promoting epidermal cell renewal and stimulating collagen synthesis. Sodium hyaluronate has good moisturizing effects. Sodium retinoyl hyaluronate has potential moisturizing and anti-aging effects.

[0004] Medicinal plant ingredients are chemical substances with medical effects or physiological activities in traditional Chinese medicine, and have been used in China for thousands of years. Medicinal plant ingredients have been widely used in the fields of cosmetics and food processing. Cosmetics containing natural Chinese herbal active ingredients are safe to use, widely applicable, and have characteristics that cannot be replaced by chemical cosmetics.

[0005] Currently, most of the skin care products on the market claiming to be anti-aging cannot meet the needs of consumers, and some products are highly irritating to the skin, causing dry skin. It is urgent to develop anti-aging ingredients that have significant anti-aging effects, are moisturizing, mild and non-irritating. Sodium retinoyl hyaluronate is a potential moisturizing and anti-aging ingredient. Medicinal plant ingredients have good soothing and repairing effects. At present, there are no reports on the synergistic moisturizing and anti-aging effects of sodium retinoyl hyaluronate and medicinal plant ingredients. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a composition with moisturizing and anti-aging effects and its application in cosmetics. The moisturizing and anti-aging composition comprises sodium retinoyl hyaluronate and aquilegia seed extract. Experimental verification shows that sodium retinoyl hyaluronate and aquilegia seed extract have a synergistic effect and significantly promote the expression of type XVII collagen gene and the production of AQP3 protein (aquaporin 3). The composition can be used in cosmetics to improve skin dryness and aging problems. At the same time, the composition has good stability, is safe and non-irritating, and therefore has good practical promotion and application value.

[0007] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0008] The first aspect of the present invention provides a composition with moisturizing and anti-aging effects, the composition comprising sodium retinoyl hyaluronate and aquilegia seed extract, wherein, by weight, the sodium retinoyl hyaluronate is 0.01-0.2 parts and the aquilegia seed extract is 4-15 parts.

[0009] Preparation method: Mix sodium retinoyl hyaluronate and geranium seed extract together and dissolve in purified water (make up to 100 parts).

[0010] Preferably, the composition with moisturizing and anti-aging effects comprises, by weight, 0.08-0.14 parts of sodium retinoyl hyaluronate and 8-12 parts of Gynura asiatica extract.

[0011] The sodium retinoyl hyaluronate is an ester prepared by reacting carbonyl diimidazole, retinoic acid, isotretinoin and hyaluronic acid. Its degree of esterification (degree of substitution) DS (mol / mol) is 4.0% to 7.0%, the retinoic acid / isoretinoic acid K (mol / mol) combined with HA is 1.6 to 2.3, and the molecular weight is 5KDa to 500KDa. Specifically: weigh an appropriate amount of carbonyl diimidazole, retinoic acid and isotretinoin and dissolve them in dimethyl sulfoxide, and the solution is stirred and reacted at below 60°C for 10-14h. Weigh an appropriate amount of hyaluronic acid and dissolve it in dimethylformamide or dimethyl sulfoxide at 65-75°C, and stir at a constant temperature for 20-40min. The molar ratio of carbonyl diimidazole, retinoic acid, isotretinoin and hyaluronic acid is 28-32:0.4-0.6:1. The solution of carbonyldiimidazole, retinoic acid and isotretinoin is added dropwise to the hyaluronic acid solution, reacted at 65-75°C for 5-8 hours, and then diluted with saturated sodium chloride solution. Ethanol is added for precipitation and washing. The precipitate is collected after filtration and dried under reduced pressure to obtain a light yellow to yellow granular powder.

[0012] The preparation method of the aquilegia seed extract is as follows: aquilegia seed powder is extracted by heating and refluxing with a 40-60% ethanol solution, the extract is concentrated under reduced pressure to a density of 1.0-1.2 g / mL, and an appropriate amount of butanediol is added to the extract until the concentration is 0.05-0.12 g / mL (calculated as crude drug amount), thereby obtaining the aquilegia seed extract.

[0013] The second aspect of the present invention provides the use of the above composition in the preparation of moisturizing, anti-aging and skin care cosmetics. The present invention has verified through experiments that the above composition can synergistically promote the expression of type XVII collagen gene and the production of AQP3 protein; at the same time, it is safe and non-irritating, so it can be used in moisturizing, anti-aging and skin care cosmetics.

[0014] Therefore, the third aspect of the present invention provides a moisturizing, anti-aging and skin care cosmetic, which comprises the above composition.

[0015] The moisturizing, anti-aging and skin care cosmetics may also contain any other raw material ingredients permitted in the cosmetics field, including but not limited to emulsifiers, emollients, moisturizers and thickeners.

[0016] Furthermore, based on the total mass of the moisturizing and anti-aging skin care cosmetic, the amount of the composition with moisturizing and anti-aging effects is 0.1-7.0%, preferably 0.5-3.0%.

[0017] At the same time, the present invention can also prepare different cosmetic dosage forms, such as essence water, essence lotion, essence cream, etc., by reasonably adding the above-mentioned raw material ingredients. At the same time, based on the above-mentioned basic cosmetic categories, the cosmetic categories obtained by further derivation and preparation obviously all fall within the scope of protection of this application.

[0018] Beneficial effects of one or more of the above technical solutions:

[0019] (1) The moisturizing and anti-aging composition obtained by compounding sodium retinoyl hyaluronate and kelp seed extract of the present invention has a significant synergistic effect, and achieves good moisturizing and anti-aging effects by promoting the expression of type XVII collagen gene and the production of AQP3 protein;

[0020] (2) The sodium retinoyl hyaluronate in the moisturizing and anti-aging composition has a slight skin irritation, and the skin irritation of the sodium retinoyl hyaluronate can be significantly reduced by combining it with the extract of Glechoma longifolia.

[0021] (3) The experiments have shown that the extract of Gastrodia elata seed ② (water extraction and alcohol precipitation method) and sodium retinoyl hyaluronate do not have a synergistic effect in moisturizing and anti-aging. The combination of Gastrodia elata seed extract ① (reflux extraction method) and sodium retinoyl hyaluronate can make the product have a higher moisturizing and anti-aging effect. This shows that the extraction method of Gastrodia elata seed is very critical.

[0022] (4) The moisturizing and anti-aging skin care cosmetics of the present invention have a simple and easy preparation method, and the raw materials are easy to obtain, which is suitable for mass production. They have passed human efficacy experiments and have good moisturizing and anti-aging effects, and therefore have good practical application value. DETAILED DESCRIPTION

[0023] The present invention is further described below by examples, but the present invention is not limited to the scope of the embodiments. Based on the embodiments in the present invention, any changes to the present invention by those skilled in the art without creative work are all within the scope of the present invention. Meanwhile, in the embodiments of the present invention, unless otherwise specified, all preparation raw materials are commercially available products well known to those skilled in the art.

[0024] Examples 1-5 and Comparative Examples 1-18:

[0025] The specific schemes of Examples 1-5 and Comparative Examples 1-18 are shown in Table 1 below, calculated by mass fractions. The preparation method is: Sodium retinoyl hyaluronate and Glechoma longituba extract (Comparative Examples are replaced with corresponding ingredients according to Table 1) are mixed together and dissolved in purified water.

[0026] The preparation method of sodium retinoyl hyaluronate comprises the following specific steps:

[0027] Weigh an appropriate amount of carbonyldiimidazole, retinoic acid and isotretinoin and dissolve them in dimethyl sulfoxide. The solution is stirred and reacted for 12 hours at below 60°C. Weigh an appropriate amount of hyaluronic acid and dissolve it in dimethylformamide at 70°C, and stir at constant temperature for 30 minutes. The molar ratio of carbonyldiimidazole, retinoic acid, isotretinoin and hyaluronic acid is 30:0.5:1. Add the solution of carbonyldiimidazole, retinoic acid and isotretinoin to the hyaluronic acid solution, react at 70°C for 6 hours, add saturated sodium chloride solution to dilute, add ethanol to precipitate and wash, collect the precipitate after filtration, and dry under reduced pressure to obtain a light yellow to yellow granular powder, and obtain a retinoyl hyaluronic acid sodium with an esterification degree (substitution degree) DS (mol / mol) of 5.0%, a retinoic acid / isoretinoic acid K (mol / mol) bound to HA of 1.8, and a molecular weight of 8KDa.

[0028] The preparation method of the Achyranthes bidentata seed extract comprises the following specific steps:

[0029] The extraction method of Gastrodia elata extract ① is as follows:

[0030] (1) Wash the seeds of Asparagus cochinchinensis with deionized water, dry them at 40°C, grind them, pass them through an 80-mesh sieve, and place them in a desiccator for later use;

[0031] (2) Accurately weigh an appropriate amount of Gastrodia elata powder, place it in a round-bottom flask, heat and reflux for extraction, the extraction solvent is 50% ethanol solution, the first solid-liquid ratio is 1:10, the second solid-liquid ratio is 1:6, and the two filtrates are combined. The filtrate is concentrated under reduced pressure to a density of 1.1574 g / mL, and an appropriate amount of butanediol is added to the extract concentration of 0.1012 g / mL (based on the amount of crude drug), thereby obtaining Gastrodia elata extract①.

[0032] The extraction method of the extract of Gastrodia elata seeds is as follows:

[0033] (1) Wash the seeds of Gastrodia elata with deionized water, dry them at 40°C, grind them, pass them through an 80-mesh sieve, and place them in a desiccator for later use.

[0034] (2) Accurately weigh an appropriate amount of Radix Glechomae Semen, add 10 times the amount of water, decoct for 3 times, 60 min each time, combine the decoctions, and concentrate under reduced pressure at 65°C to a density of 1.1042 g / mL. Cool, add alcohol to make the alcohol concentration reach 70%, refrigerate and let stand for 12 h. After standing, filter, take the upper layer of liquid and concentrate under reduced pressure to a density of 1.1615 g / mL. Add an appropriate amount of butanediol to the extract concentration of 0.1037 g / mL (based on the amount of crude drug), and obtain Radix Glechomae Semen Extract②.

[0035] Table 1 Specific examples and comparative examples ingredient list

[0036]

[0037]

[0038] Example 6: Essence Cream

[0039] The above composition with moisturizing and anti-aging effects is used to prepare an essence cream product containing the composition with moisturizing and anti-aging effects, and the formula is shown in Table 2.

[0040] Table 2 Essence cream product formula

[0041]

[0042] Preparation method of essence cream:

[0043] S1: Preparation of aqueous phase: Add the raw materials in phase A respectively, heat to 85°C and stir to dissolve;

[0044] S2: Preparation of oil phase: Add the raw materials in phase B respectively and heat to 80°C to melt;

[0045] S3: Combine phase A and phase B, start homogenization (3000r / min), and homogenize for 12min;

[0046] S4: After homogenization, start cooling down. When the temperature drops to 70°C, add phase C and stir to dissolve. After dissolution, continue cooling down.

[0047] S5: When the temperature drops to 45°C, add the phase D ingredients, stir to dissolve and disperse evenly to obtain the essence cream product.

[0048] Test Example 1: Irritation Evaluation Test

[0049] The experiment recruited 30 subjects (7 males and 23 females), aged 18 to 60 years old.

[0050] Human skin closed patch test method: Dilute the test samples of Examples 1-5 and Comparative Examples 5, 10, 11, 13, 15, and 17 with distilled water into a 1% solution as the test substance, and distilled water as the control group. Select a qualified patch tester and place 0.020-0.025 mL of the test substance into the small chamber of the patch tester. Apply the patch tester with the test substance to the back of the subject for 24 hours. After 24 hours, remove the test substance patch tester, observe the skin reaction at 0.5 hours, 24 hours, and 48 hours after removal, and record the results according to the skin reaction grading standard (Table 3) in the "Cosmetic Safety Technical Specifications 2015". Detailed results are shown in Table 4. Explain the results according to the "Cosmetic Hygiene Specifications (2007 Edition)". The interpretation criteria for the results of the skin occlusive patch test are as follows: among 30 subjects, if more than 5 cases of Grade 1 adverse skin reactions occur, or more than 2 cases of Grade 2 adverse skin reactions occur, or if any case of Grade 3 or above adverse skin reaction occurs, the test substance is judged to have adverse skin reactions to humans.

[0051] Table 3 Skin reaction grading standards

[0052]

[0053] The test results are shown in Table 4. Examples 1-5 and Comparative Examples 10, 11, and 15 showed no adverse reactions and were mild and non-irritating. Comparative Examples 5, 13, and 17 all showed adverse reactions to varying degrees, indicating that sodium retinoyl hyaluronate alone, sodium retinoyl hyaluronate in combination with Achyranthes seed extract ②, and 0.4 parts of sodium retinoyl hyaluronate in combination with Achyranthes seed extract ① were irritating to the skin.

[0054] Table 4 Irritation evaluation test results

[0055]

[0056]

[0057] Test Example 2: In vitro anti-aging efficacy study

[0058] The experiment used human skin fibroblasts (HSF) as a model cell line. After the cells were revived, when the plating rate reached about 60%, the cells were inoculated into a 6-well plate and incubated overnight in a CO2 incubator (37°C, 5% CO2). The working solution of the test substance was prepared according to the test scheme (Table 5). When the cell plating rate in the 6-well plate reached 40% to 60%, the group administration was carried out, and 3 replicates were set up in each group. The culture medium was DMEM culture medium, 2 mL of culture medium was added to each well of the blank control group, 2 mL of culture medium containing TGF-β1 was added to each well of the positive control group, and 2 mL of culture medium containing the corresponding concentration of the sample to be tested was added to each well of the sample group. After the administration was completed, the 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) for 24 hours. After 24 hours of culture and incubation, the old solution was discarded, PBS was washed twice, 1 mL of RNAiso Plus was added to each well, and the cells were lysed by blowing and collecting the samples. RNA was extracted and reverse transcribed into cDNA, and then fluorescent quantitative PCR was performed. -△△CT Methods The results of Collagen XVII gene were calculated. The formula for calculating the up-regulation rate of Collagen XVII is as follows.

[0059]

[0060] Using the COLBY formula to verify synergy: Where X and Y are the test results of a single component, respectively, and E is calculated according to the COLBY formula. 测定 is the result of the composition test, E 测定 >E 计算 Expressed as synergy, E 测定 =E 计算 Expressed as a sum, E 测定 <E 计算 Indicated as no synergistic effect.

[0061] GraphPad Prism was used for graphing, and the results were expressed as Mean ± SD. The t-test was used for statistical analysis between the groups. All statistical analyses were two-tailed. P < 0.05 was considered to be significantly different, and P < 0.01 was considered to be extremely significantly different.

[0062] Table 5 Test plan

[0063]

[0064] Table 6 Summary of Collagen XVII Gene Detection Results

[0065]

[0066]

[0067] Table 7 Summary of in vitro anti-aging synergistic effects

[0068]

[0069]

[0070] It can be seen from Tables 6 and 7 that the E 测定 >E 计算 , indicating that the combination of sodium retinoyl hyaluronate and geranium seed extract ① in Examples 1-5 has a synergistic effect in promoting the expression of type XVII collagen gene, that is, has a synergistic anti-aging effect, and the synergistic effect of Example 3 is the most significant. 测定 <E 计算 , indicating that the combination of dimethylsilanol hyaluronate and Aquilegia odorata extract ① in comparative example 11 does not have the effect of synergistically promoting the expression of type XVII collagen gene; the combination of sodium retinoyl hyaluronate and Aquilegia odorata extract ② in comparative example 13 does not have the effect of synergistically promoting the expression of type XVII collagen gene; the combination of sodium retinoyl hyaluronate and 20 parts of Aquilegia odorata extract ① in comparative example 15 does not have the effect of synergistically promoting the expression of type XVII collagen gene; comparative example 17 indicates that the combination of 0.4 parts of sodium retinoyl hyaluronate and Aquilegia odorata extract ① does not have the effect of synergistically promoting the expression of type XVII collagen gene.

[0071] Test Example 3: In vitro moisturizing (AQP3 enhancement) efficacy study

[0072] The experiment used keratinocytes as a model cell line for research. After the cells were revived, when the plating rate reached about 60%, the cells were inoculated into a 24-well plate and incubated overnight in a CO2 incubator (37°C, 5% CO2). The working solution of the test substance was prepared according to the test scheme (Table 8). According to the test grouping, when the cell plating rate in the 24-well plate reached 40% to 60%, group medication was carried out, and each group had 3 replicate wells. The culture medium was KcGrowth culture medium. 1 mL of culture medium was added to each well of the blank control group, 1 mL of culture medium containing CaCl2 was added to each well of the positive control group, and 1 mL of culture medium containing the sample to be tested at the corresponding concentration was added to each well of the sample group. After the administration was completed, the 24-well plate was placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24 hours.

[0073] The cells were fixed with 4% paraformaldehyde for 30 minutes, and then immunofluorescence detection was performed. The cells were observed under a microscope, and the images were collected and analyzed. The calculation formula for the AQP3 elevation rate is as follows.

[0074]

[0075] Use the COLBY formula to verify synergy:

[0076] GraphPad Prism was used for graphing, and the results were expressed as Mean ± SD. The t-test was used for statistical analysis between the groups. All statistical analyses were two-tailed. P < 0.05 was considered to be significantly different, and P < 0.01 was considered to be extremely significantly different.

[0077] Table 8 Test plan

[0078]

[0079] Table 9: Summary of AQP3 immunofluorescence analysis results

[0080] Sample name Average concentration (ng / mL) SD P-value Improvement rate (vsBC) BC 0.94 0.06 / / PC 1.39 0.15 0.008 47.87％ Example 1 1.33 0.08 0.003 41.49％ Example 2 1.49 0.12 0.002 58.51％ Example 3 1.76 0.21 0.003 87.23％ Example 4 1.56 0.16 0.003 65.96％ Example 5 1.45 0.11 0.002 54.26％ Comparative Example 1 1.10 0.06 0.031 17.02％ Comparative Example 2 1.13 0.05 0.014 20.21％ Comparative Example 3 1.20 0.11 0.023 27.66％ Comparative Example 4 1.25 0.15 0.029 32.98％ Comparative Example 5 1.27 0.09 0.006 35.11％ Comparative Example 6 1.00 0.05 0.254 6.38％ Comparative Example 7 1.03 0.08 0.194 9.57％ Comparative Example 8 1.04 0.07 0.133 10.64％ Comparative Example 9 1.03 0.08 0.194 9.57％ Comparative Example 10 1.04 0.07 0.133 10.64％ Comparative Example 11 1.30 0.12 0.010 38.30％ Comparative Example 12 1.27 0.11 0.010 35.11％ Comparative Example 13 1.18 0.09 0.018 25.53％ Comparative Example 14 0.96 0.04 0.656 2.13％ Comparative Example 15 1.30 0.13 0.012 38.30％ Comparative Example 16 1.03 0.06 0.140 9.57％ Comparative Example 17 1.37 0.15 0.010 45.74％ Comparative Example 18 1.29 0.17 0.028 37.23％

[0081] Table 10 Summary of in vitro moisturizing synergy

[0082]

[0083]

[0084]

[0085] The test results are shown in Tables 9 and 10. 测定 >E 计算 , indicating that the combination of sodium retinoyl hyaluronate and kelp seed extract ① in Examples 1-5 can synergistically promote the production of AQP3 protein, that is, has a synergistic moisturizing effect, and the synergistic effect of Example 3 is the most significant. 测定 <E 计算 , indicating that the combination of dimethylsilanol hyaluronate and Aquilegia odorata extract ① in comparative example 11 does not have the effect of synergistically promoting the production of AQP3 protein; the combination of sodium retinoyl hyaluronate and Aquilegia odorata extract ② in comparative example 13 does not have the effect of synergistically promoting the production of AQP3 protein; the combination of sodium retinoyl hyaluronate and 20 parts of Aquilegia odorata extract ① in comparative example 15 does not have the effect of synergistically promoting the production of AQP3 protein; comparative example 17 indicates that the combination of 0.4 parts of sodium retinoyl hyaluronate and Aquilegia odorata extract ① does not have the effect of synergistically promoting the production of AQP3 protein.

[0086] Test Example 4: Test results of the moisturizing and anti-aging experiment on human body using the moisturizing and anti-aging composition of the present invention

[0087] Example 6 was tested for its moisturizing and anti-aging efficacy on human skin, and a total of 30 people completed the test, including 8 males and 22 females aged 25 to 55 years old, meeting the voluntary inclusion and exclusion criteria of the subjects.

[0088] How to use: After using toner, take an appropriate amount of sample and apply it evenly on the face, pat until absorbed, and then carry out normal skin care steps. Use it once in the morning and evening every day. Keep other skin care habits unchanged and use it continuously for 28 days.

[0089] Detection time: before using the sample (D0), 7 days after using the sample (D7), and 28 days after using the sample (D28).

[0090] Test environment: Temperature: 20.0℃-22℃; Relative humidity: 40%-60%.

[0091] Test method:

[0092] 1. Skin Moisture Content Tester CM825 is used to detect the relative moisture content of the skin surface.

[0093] 2. Skin elasticity tester MPA580 is used to detect skin elasticity.

[0094] Experimental parameters:

[0095] 1. Skin moisture content: The larger the skin moisture content, the higher the skin moisture content and the better the moisturizing effect.

[0096] 2. Skin elasticity: The larger the skin elasticity R2 and R7 values, the better the skin elasticity and the better the anti-aging effect.

[0097] Test requirements: Test 3 times and take the average value.

[0098] The results are shown in Tables 11-13.

[0099] Table 11 Descriptive statistics of skin moisture content (n=30)

[0100]

[0101]

[0102] Statistical analysis results: “***”: the difference is statistically significant (P<0.001); “****”: the difference is statistically significant (P<0.0001).

[0103] As shown in Table 11, the skin moisture content was significantly increased after 7 days and 28 days of using Example 6, which has a good moisturizing effect.

[0104] Table 12 Descriptive statistics of skin elasticity R2 value (n=30)

[0105]

[0106] Statistical analysis results: “***”: the difference is statistically significant (P<0.001); “****”: the difference is statistically significant (P<0.0001).

[0107] Table 13 Descriptive statistics of skin elasticity R7 value (n=30)

[0108]

[0109] Statistical analysis results: “**”: the difference is statistically significant (0.001≤P<0.01); “***”: the difference is statistically significant (P<0.001).

[0110] As shown in Tables 12 and 13, the skin elasticity of R2 and R7 was significantly improved after 7 days and 28 days of using Example 6, which has good anti-aging effect.

[0111] It should be noted that the above examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art may modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A composition with moisturizing and anti-aging effects, characterized in that: The composition comprises sodium retinoyl hyaluronate and abalone seed extract, wherein, by weight, the sodium retinoyl hyaluronate comprises 0.01-0.2 parts and the abalone seed extract comprises 4-15 parts.

2. A composition with moisturizing and anti-aging effects as claimed in claim 1, characterized in that: Calculated by weight, the ingredients include 0.08-0.14 parts of sodium retinoyl hyaluronate and 8-12 parts of Gastrodia elata seed extract.

3. A composition with moisturizing and anti-aging effects as claimed in claim 1, characterized in that: The sodium retinoyl hyaluronate is an ester prepared by reacting carbonyldiimidazole, retinoic acid, isotretinoin and hyaluronic acid, with an esterification degree of 4.0% to 7.0%, a retinoic acid / isotretinoin molar ratio of HA to 1.6 to 2.3, and a molecular weight of 5KDa to 500KDa.

4. The composition with moisturizing and anti-aging effects as claimed in claim 1, characterized in that: The preparation method of the aquilegia seed extract is as follows: extracting aquilegia seed powder by heating and refluxing with a 40-60% ethanol solution, concentrating the extract under reduced pressure to a density of 1.0-1.2 g / mL, and then adding an appropriate amount of butanediol to a concentration of 0.05-0.12 g / mL in terms of crude drug amount, thereby obtaining the aquilegia seed extract.

5. The composition with moisturizing and anti-aging effects as claimed in claim 1, characterized in that: Mix sodium retinoyl hyaluronate and geranium seed extract together and dissolve in water to make up to 100 parts.

6. Use of the composition with moisturizing and anti-aging effects according to any one of claims 1 to 5 in the preparation of moisturizing and anti-aging skin care cosmetics.

7. The use according to claim 6, characterized in that: By promoting the expression of type XVII collagen gene and the production of AQP3 protein, it achieves the effect of moisturizing and anti-aging.

8. A moisturizing, anti-aging and skin care cosmetic, characterized in that: A composition with moisturizing and anti-aging effects comprising any one of claims 1 to 5.

9. The moisturizing, anti-aging and skin-care cosmetic according to claim 8, characterized in that: The amount of the moisturizing and anti-aging composition according to claim 5 is 0.1-7.0%.

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