A composition and its application in preparing products with anti-wrinkle effect

Abstract

The present invention provides a composition and its application in preparing a product with anti-wrinkle effect, the composition comprises the following components by weight: 10-20 parts of hydroxypropyl tetrahydropyrantriol, 35-45 parts of bacillus / soybean fermentation product extract, 3-6 parts of folic acid, 4-10 parts of sodium hyaluronate, 5-10 parts of tripeptide-1, 15-25 parts of acetyl hexapeptide-8, 3-8 parts of pentapeptide-3, 4-8 parts of amino acid mixture, 2-5 parts of fucus extract and 7-12 parts of soluble collagen; the amino acid mixture comprises 0.1-0.2 parts of glutamic acid, 0.1-0.3 parts of histidine, 0.5-1.5 parts of acetylhydroxyproline, 0.3-0.8 parts of arginine and 1-3 parts of aspartic acid; the molecular weight of the soluble collagen is 8000-12000. The composition has significant anti-wrinkle and anti-oxidation effects, and has a long duration, and can effectively solve the problems existing in existing products.

Description

Technical Field

[0001] The invention belongs to the technical field of anti-wrinkle cosmetics, and specifically relates to a composition and application thereof in preparing products with anti-wrinkle effects. Background Art

[0002] Generally, after the age of 25, women's skin metabolism slows down, the balance between cell proliferation, differentiation, aging and disappearance is broken, and the body's ability to remove free radicals decreases. In addition, due to the influence of ultraviolet rays, electromagnetic radiation and other factors, free radicals and reactive oxygen will accumulate in the human body. These two substances will deform the protein in the skin, causing it to lose its original function, which in turn causes a decrease in skin elasticity and wrinkles.

[0003] Anti-wrinkle products are an important category of products in the field of beauty and skin care, which are designed to reduce skin wrinkles, improve skin quality and delay aging. As people pursue beauty and attach importance to anti-aging, the market demand for anti-wrinkle products continues to grow.

[0004] Current anti-wrinkle cosmetics mainly contain ingredients such as collagen or hydrolyzed collagen, retinol, etc. Although these products can produce anti-wrinkle effects in a short period of time, they cannot play an anti-wrinkle and firming role for a long time; some anti-wrinkle products are unstable and easily oxidized, and some even have side effects, causing the local skin sensitivity to decrease. Summary of the invention

[0005] The present invention aims to provide a composition and its application in preparing a product with anti-wrinkle effect, which is prepared by adding hydroxypropyl tetrahydropyrantriol, bacillus / soybean fermentation product extract, soluble collagen and other ingredients; the composition has significant anti-wrinkle effect, long duration, small side effects, and can effectively solve the problems of existing products.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A composition comprises the following components in parts by weight: 10-20 parts of hydroxypropyl tetrahydropyrantriol, 35-45 parts of bacillus / soybean fermentation product extract, 3-6 parts of folic acid, 4-10 parts of sodium hyaluronate, 5-10 parts of tripeptide-1, 15-25 parts of acetyl hexapeptide-8, 3-8 parts of pentapeptide-3 and 7-12 parts of soluble collagen.

[0007] Furthermore, 4-8 parts of amino acid mixture are included.

[0008] Furthermore, the amino acid mixture includes 0.1-0.2 parts of glutamic acid, 0.1-0.3 parts of histidine, 0.5-1.5 parts of acetylhydroxyproline, 0.3-0.8 parts of arginine and 1-3 parts of aspartic acid.

[0009] Furthermore, 2-5 portions of Fucus vesiculosus extract are also included.

[0010] Furthermore, the molecular weight of the soluble collagen is 8000-12000.

[0011] A method for preparing a composition comprises the following steps: S1: Preparation of Bacillus / soybean fermentation product extract; S2: preparing amino acid mixture and Fucus vesiculosus extract; S3: First, 5-10 parts of tripeptide-1, 15-25 parts of acetyl hexapeptide-8, and 3-8 parts of pentapeptide-3 are mixed, and stirred at a temperature of 25-35° C. for 15-25 minutes to obtain a mixture A; S4: 35-45 parts of the Bacillus / soybean fermentation product extract, 7-12 parts of soluble collagen, 4-8 parts of the amino acid mixture and 2-5 parts of the Fucus vesiculosus extract in step S1 are stirred at a temperature of 50-60° C. for 20-30 minutes to obtain a mixture B; S5: 10-20 parts of hydroxypropyl tetrahydropyrantriol, 3-6 parts of folic acid, 4-10 parts of sodium hyaluronate, the mixture A obtained in step S3 and the mixture B obtained in step S4 are mixed, and stirred at a temperature of 34-45° C. for 2-4 hours to obtain the composition.

[0012] Furthermore, the preparation process of the Bacillus / soybean fermentation product extract in step S1 is as follows: adding 80-120 parts of water to 20-40 parts of ground soybean powder, adding 1-2 parts of protease, performing enzymatic hydrolysis at a temperature of 40-50°C for 1-2 hours, and inactivating the enzyme at a temperature of 80-90°C for 15-25 minutes to obtain a soybean powder hydrolysate; taking 1-5% of the soybean powder hydrolysate, 1-3% of sodium glutamate, 1-3% of glucose, 0.2-0.5% of sodium chloride and 60-80 parts of water to mix to obtain a culture medium; inoculating the Bacillus seed liquid into the culture medium, fermenting at a temperature of 40-60°C for 50-80 minutes, and obtaining the Bacillus / soybean fermentation product extract after sterilization.

[0013] Furthermore, the preparation process of the amino acid mixture in step S2 is: first, 0.5-1 parts of arginine and 1-2 parts of serine are added to 30-50 parts of water, and completely dissolved at room temperature to obtain liquid A; 1-3 parts of glutamic acid, 0.5-2.5 parts of aspartic acid and 0.8-1.3 parts of glutamine are added to 35-45 parts of water, and completely dissolved at a temperature of 50-80°C to obtain liquid B; then liquid A and liquid B are mixed, the pH value of the solution is adjusted to 6.5-7, and the mixture is stirred and mixed at a temperature of 25-35°C for 55-75 minutes to obtain an amino acid mixture.

[0014] Furthermore, the preparation process of the Fucus vesiculosus extract in step S2 is: take 10-20 parts of crushed Fucus vesiculosus, add 100-150 parts of water, 0.15-0.35 parts of hemicellulase, 0.1-0.2 parts of aminopeptidase and 0.05-0.15 parts of papain, enzymolysis at 40-45°C for 1-2h, inactivate the enzyme at 80-90°C for 30-40min, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, concentrate and dry to obtain the Fucus vesiculosus extract.

[0015] The composition prepared above is applied to products with anti-wrinkle effect.

[0016] Compared with the prior art, the advantages and beneficial effects of the present invention are: The invention uses hydroxypropyl tetrahydropyrantriol, bacillus / soybean fermentation product extract, folic acid, sodium hyaluronate, tripeptide-1, acetyl hexapeptide-8, pentapeptide-3 and soluble collagen as ingredients, and adds an amino acid composition and a fucus extract. The invention reduces muscle contraction and wrinkle generation by inhibiting the release of the neurotransmitter acetylcholine; promotes the synthesis of matrix proteins, especially collagen, and increases the generation of elastin, hyaluronic acid, glycosaminoglycan and fibronectin, thereby achieving the purpose of anti-aging and wrinkle removal, and has a long duration and good performance effect. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] The raw materials used in the present invention are as follows: Protease (Shanghai Yuanye Biotechnology Co., Ltd. Product No. S51641); monosodium glutamate (Hubei Baidu Chemical Co., Ltd. Product No. BD1527); glucose (Shandong Yonglida New Materials Technology Co., Ltd. Product No. 297); Bacillus seed solution (Aberson Biotechnology Co., Ltd. Product No. 126); arginine (Hefei Tanrun Biotechnology Co., Ltd. Product No. 320); serine (Hefei Tanrun Biotechnology Co., Ltd. Product No. 321); glutamic acid (Hefei Tanrun Biotechnology Co., Ltd. Product No. 323); aspartic acid (Hefei Tanrun Biotechnology Co., Ltd. Product No. 325); glutamine (Anhui Runtai Biotechnology Co., Ltd. Product No. 3007); hemicellulase (Shanghai Yuanye Biotechnology Co., Ltd. Technology Co., Ltd. Product No. S10045); aminopeptidase (Jiangsu Caiwei Biotechnology Co., Ltd. Product No. 251); papain (Jiangsu Caiwei Biotechnology Co., Ltd. Product No. 213); tripeptide-1 (Shanghai Yuanye Biotechnology Co., Ltd. Product No. Y42203); acetyl hexapeptide-8 (Shanghai Yuanye Biotechnology Co., Ltd. Product No. S80841); pentapeptide-3 (Zhengpeptide Biochemical Technology (Nanjing) Co., Ltd. Product No. 1144); hydroxypropyl tetrahydropyrantriol (Shanghai Yuanye Biotechnology Co., Ltd. Product No. S29957); folic acid (Hebei Yaxin Biotechnology Co., Ltd. Product No. 880); sodium hyaluronate (Shanghai Yuanye Biotechnology Co., Ltd. Product No. S12034).

[0019] Example 1 This embodiment provides a method for preparing a composition, comprising the following steps: S1: Add 20g of ground soybean powder to 80g of water, then add 1g of protease, perform enzymatic hydrolysis at 40°C for 1h, and inactivate the enzyme at 80°C for 15min to obtain soybean powder enzymatic hydrolysate; mix 1g of soybean powder enzymatic hydrolysate, 1g of sodium glutamate, 1g of glucose, 0.2g of sodium chloride and 60g of water to obtain a culture medium; inoculate 5ml of Bacillus seed liquid into the culture medium, ferment at 40°C for 50min, and obtain a Bacillus / soybean fermentation product extract after sterilization; S2: First, add 0.5g arginine and 1g serine to 30g water and dissolve them completely at room temperature to obtain liquid A; add 1g glutamic acid, 0.5g aspartic acid and 0.8g glutamine to 35g water and dissolve them completely at 50°C to obtain liquid B; then mix liquid A and liquid B, adjust the pH value of the solution to 6.5, and stir and mix at a speed of 500r / min for 55min at a temperature of 25°C to obtain an amino acid mixture; Take 10g of the crushed Fucus vesiculosus, add 100g of water, 0.15g of hemicellulase, 0.1g of aminopeptidase and 0.05g of papain, enzymolyze at 40°C for 1h, inactivate the enzyme at 80°C for 30min, filter with a 2kDa ultrafiltration membrane, take the filtrate with a content less than 2kDa, concentrate and dry, and obtain the Fucus vesiculosus extract; S3: First, 5 g of tripeptide-1, 15 g of acetyl hexapeptide-8, and 3 g of pentapeptide-3 were mixed, and stirred at a temperature of 25°C and a speed of 600 r / min for 15 min to obtain a mixture A; S4: 35 g of the Bacillus / soybean fermentation product extract, 7 g of soluble collagen, 4 g of the amino acid mixture and 2 g of the Fucus vesiculosus extract in step S1 were stirred at a temperature of 50° C. and a speed of 500 r / min for 20 min to obtain a mixture B; S5: 10 g of hydroxypropyl tetrahydropyrantriol, 3 g of folic acid, 4 g of sodium hyaluronate, the mixture A obtained in step S3 and the mixture B obtained in step S4 were mixed, and stirred at a temperature of 34° C. and a speed of 600 r / min for 2 h to obtain the composition.

[0020] Example 2 This embodiment provides a method for preparing a composition, comprising the following steps: S1: 40g of ground soybean powder was added to 120g of water, and then 2g of protease was added, and the enzymatic reaction was carried out at a temperature of 50°C for 2h, and the enzyme was inactivated at a temperature of 90°C for 25min to obtain a soybean powder enzymatic solution; 5g of soybean powder enzymatic solution, 3g of sodium glutamate, 3g of glucose, 0.5g of sodium chloride and 80g of water were mixed to obtain a culture medium; 4ml of Bacillus seed liquid was inoculated into the culture medium, and fermented at a temperature of 60°C for 80min, and a Bacillus / soybean fermentation product extract was obtained after sterilization; S2: First, add 1g arginine and 2g serine to 50g water and dissolve them completely at room temperature to obtain liquid A; add 3g glutamic acid, 2.5g aspartic acid and 1.3g glutamine to 45g water and dissolve them completely at 80°C to obtain liquid B; then mix liquid A and liquid B, adjust the pH value of the solution to 7, and stir and mix at a temperature of 35°C at a speed of 400r / min for 75min to obtain an amino acid mixture; Take 20g of the crushed Fucus vesiculosus, add 150g of water, 0.35g of hemicellulase, 0.2g of aminopeptidase and 0.15g of papain, enzymolyze at 45°C for 2h, inactivate the enzyme at 90°C for 40min, filter with a 2kDa ultrafiltration membrane, take the filtrate with a content less than 2kDa, concentrate and dry, and obtain a Fucus vesiculosus extract; S3: First, 10 g of tripeptide-1, 25 g of acetyl hexapeptide-8, and 8 g of pentapeptide-3 were mixed, and stirred at a temperature of 35°C and a speed of 500 r / min for 25 min to obtain a mixture A; S4: 45 g of the Bacillus / soybean fermentation product extract, 12 g of soluble collagen, 8 g of the amino acid mixture and 5 g of the Fucus vesiculosus extract in step S1 are stirred at 60° C. and 500 r / min for 30 min to obtain a mixture B; S5: 20 g of hydroxypropyl tetrahydropyrantriol, 6 g of folic acid, 10 g of sodium hyaluronate, the mixture A obtained in step S3 and the mixture B obtained in step S4 were mixed, and stirred at a temperature of 45° C. and a speed of 400 r / min for 4 h to obtain the composition.

[0021] Example 3 This embodiment provides a method for preparing a composition, comprising the following steps: S1: 35g of ground soybean powder was added to 100g of water, and then 1.5g of protease was added, and the enzymatic reaction was carried out at a temperature of 45°C for 1.5h, and the enzyme was inactivated at a temperature of 85°C for 22min to obtain a soybean powder hydrolysate; 3g of soybean powder hydrolysate, 2g of sodium glutamate, 2g of glucose, 0.4g of sodium chloride and 75g of water were mixed to obtain a culture medium; 3ml of Bacillus seed liquid was inoculated into the culture medium, and fermented at a temperature of 55°C for 70min, and a Bacillus / soybean fermentation product extract was obtained after sterilization; S2: First, add 0.7g arginine and 1.5g serine to 45g water and dissolve them completely at room temperature to obtain liquid A; add 2g glutamic acid, 1.5g aspartic acid and 1g glutamine to 40g water and dissolve them completely at 70°C to obtain liquid B; then mix liquid A and liquid B, adjust the pH value of the solution to 6.8, and stir and mix at a speed of 500r / min for 65min at a temperature of 30°C to obtain an amino acid mixture; Take 15g of the crushed Fucus vesiculosus, add 120g of water, 0.2g of hemicellulase, 0.15g of aminopeptidase and 0.1g of papain, enzymolyze at 43°C for 1.5h, inactivate the enzyme at 85°C for 35min, filter with a 2kDa ultrafiltration membrane, take the filtrate with a volume less than 2kDa, concentrate and dry, and obtain a Fucus vesiculosus extract; S3: First, 7 g of tripeptide-1, 20 g of acetyl hexapeptide-8, and 6 g of pentapeptide-3 were mixed, and stirred at a temperature of 30°C and a speed of 600 r / min for 22 min to obtain a mixture A; S4: 40 g of the Bacillus / soybean fermentation product extract, 10 g of soluble collagen, 7 g of the amino acid mixture and 4 g of the Fucus vesiculosus extract in step S1 were stirred at a temperature of 56° C. and a speed of 500 r / min for 25 min to obtain a mixture B; S5: 15 g of hydroxypropyl tetrahydropyrantriol, 4 g of folic acid, 7 g of sodium hyaluronate, the mixture A obtained in step S3 and the mixture B obtained in step S4 were mixed, and stirred at a temperature of 40° C. and a speed of 550 r / min for 3.5 h to obtain the composition.

[0022] Comparative Example 1 The difference from Example 1 is that step S1 is omitted, that is, the Bacillus / soybean fermentation product extract is not added to the composition, and the other steps remain unchanged.

[0023] Comparative Example 2 The difference from Example 1 is that no amino acid mixture is added in step S2, and the other steps remain unchanged.

[0024] Comparative Example 3 The difference from Example 1 is that the Fucus vesiculosus extract is not added in step S2, and the other steps remain unchanged.

[0025] Comparative Example 4 The difference from Example 1 is that aspartic acid and glutamine are missing in step S2, and the other steps remain unchanged.

[0026] Comparative Example 5 The difference from Example 1 is that aminopeptidase and papain are missing in step S2, and the other steps remain unchanged.

[0027] The compositions of the embodiments and comparative examples of the present invention are applied to anti-wrinkle essence, and the preparation method thereof is as follows: 7 g of propylene glycol as a moisturizer and 0.2 g of xanthan gum as a thickener are added to water, stirred at 85° C. for 20 min, and cooled to 40° C.; 25 g of the anti-wrinkle composition and 0.5 g of benzyl alcohol as a preservative are added thereto, and the mixture is stirred and mixed at a rate of 500 r / min at room temperature for 1 h to obtain the anti-wrinkle essence.

[0028] Performance Testing: Skin moisturizing and anti-wrinkle effect detection: A total of 55 female subjects (26-47 years old) were selected and divided into 11 groups, with 5 people in each group. Each group used the products of Examples 1-3 and Comparative Examples 1-5 respectively, and applied them evenly on the face, once in the morning and once in the evening; the skin elasticity was measured using a skin elasticity meter before use, after 14 days of use, and after 30 days of use. Detection of DPPH free radical scavenging effect: Use purified water to dilute each compound into a sample solution with a mass concentration of 6%, take 3.5mL of 0.3mmol / L DPPH-anhydrous ethanol solution and 1mL of sample solution in a cuvette, place it at room temperature and away from light for 40min, and measure the absorbance at a wavelength of 517nm, recorded as N1; take 3.5mL of anhydrous ethanol solution and 1mL of sample solution in a cuvette, place it at room temperature and away from light for 40min, and measure the absorbance at a wavelength of 517nm, recorded as N2; take 3.5mL of 0.3mmol / L DPPH-anhydrous ethanol solution and 1mL of anhydrous ethanol solution in a cuvette, place it at room temperature and away from light for 40min, and measure the absorbance at a wavelength of 517nm, recorded as N3; make three parallel samples for each and take the average value. DPPH free radical scavenging rate (%) = [1-(N1-N2) / N3]×100% detection. The results are shown in Table 1 below.

[0029] Table 1

[0030] It can be seen from the above performance test results that the compositions prepared in Examples 1-3 have excellent anti-oxidation and anti-wrinkle properties. In particular, the comprehensive performance of Example 3 is the most outstanding. However, since Comparative Examples 1-5 do not adopt the necessary technical solutions, their corresponding performance tests are significantly worse than those of the Examples. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0031] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition, characterized in that Contains the following components in parts by weight: 10-20 parts hydroxypropyl tetrahydropyrantriol, 35-45 parts bacillus / soybean fermentation product extract, 3-6 parts folic acid, 4-10 parts sodium hyaluronate, 5-10 parts tripeptide-1, 15-25 parts acetyl hexapeptide-8, 3-8 parts pentapeptide-3 and 7-12 parts soluble collagen.

2. A composition according to claim 1, characterized in that Also includes 4-8 servings of an amino acid blend.

3. A composition according to claim 1, characterized in that The amino acid mixture includes 0.1-0.2 parts of glutamic acid, 0.1-0.3 parts of histidine, 0.5-1.5 parts of acetylhydroxyproline, 0.3-0.8 parts of arginine and 1-3 parts of aspartic acid.

4. A composition according to claim 1, characterized in that Also included is 2-5 portions of Fucus vesiculosus extract.

5. A composition according to claim 1, characterized in that The molecular weight of the soluble collagen is 8000-12000.

6. A method for preparing a composition, characterized in that: The steps include: S1: Preparation of Bacillus / soybean fermentation product extract; S2: preparing amino acid mixture and Fucus vesiculosus extract; S3: First, 5-10 parts of tripeptide-1, 15-25 parts of acetyl hexapeptide-8, and 3-8 parts of pentapeptide-3 are mixed, and stirred at a temperature of 25-35° C. for 15-25 minutes to obtain a mixture A; S4: 35-45 parts of the Bacillus / soybean fermentation product extract, 7-12 parts of soluble collagen, 4-8 parts of the amino acid mixture and 2-5 parts of the Fucus vesiculosus extract in step S1 are stirred at a temperature of 50-60° C. for 20-30 minutes to obtain a mixture B; S5: 10-20 parts of hydroxypropyl tetrahydropyrantriol, 3-6 parts of folic acid, 4-10 parts of sodium hyaluronate, the mixture A obtained in step S3 and the mixture B obtained in step S4 are mixed, and stirred at a temperature of 34-45° C. for 2-4 hours to obtain the composition.

7. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the Bacillus / soybean fermentation product extract in step S1 is as follows: adding 80-120 parts of water to 20-40 parts of ground soybean powder, adding 1-2 parts of protease, performing enzymatic hydrolysis at a temperature of 40-50°C for 1-2 hours, and deactivating the enzyme at a temperature of 80-90°C for 15-25 minutes to obtain a soybean powder hydrolysate; mixing 1-5% of the soybean powder hydrolysate, 1-3% of sodium glutamate, 1-3% of glucose, 0.2-0.5% of sodium chloride and 60-80 parts of water to obtain a culture medium; inoculating the Bacillus seed liquid into the culture medium, fermenting at a temperature of 40-60°C for 50-80 minutes, and obtaining the Bacillus / soybean fermentation product extract after sterilization.

8. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the amino acid mixture in step S2 is as follows: first, 0.5-1 parts of arginine and 1-2 parts of serine are added to 30-50 parts of water, and completely dissolved at room temperature to obtain liquid A; 1-3 parts of glutamic acid, 0.5-2.5 parts of aspartic acid and 0.8-1.3 parts of glutamine are added to 35-45 parts of water, and completely dissolved at a temperature of 50-80° C. to obtain liquid B; then, liquid A and liquid B are mixed, the pH value of the solution is adjusted to 6.5-7, and the mixture is stirred and mixed at a temperature of 25-35° C. for 55-75 minutes to obtain an amino acid mixture.

9. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the Fucus vesiculosus extract in step S2 is as follows: take 10-20 parts of crushed Fucus vesiculosus, add 100-150 parts of water, 0.15-0.35 parts of hemicellulase, 0.1-0.2 parts of aminopeptidase and 0.05-0.15 parts of papain, perform enzymolysis at 40-45°C for 1-2h, inactivate the enzyme at 80-90°C for 30-40min, filter with a 2kDa ultrafiltration membrane, take the filtrate less than 2kDa, concentrate and dry, and obtain the Fucus vesiculosus extract.

10. Applying the composition prepared according to claims 1 to 9 to products with anti-wrinkle effects.

Images