A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment

Abstract

The present invention belongs to the field of plant technology, specifically a multi-layer sampling device for dynamically studying changes in the rhizosphere environment of plants, comprising a hexagonal base plate, a first hexagonal enclosure integrated on the top of the hexagonal base plate, a second hexagonal enclosure integrated on the top of the hexagonal base plate located inside the first hexagonal enclosure, a third hexagonal enclosure integrated on the top of the hexagonal base plate located inside the second hexagonal enclosure, and a fourth hexagonal enclosure integrated on the top of the hexagonal base plate located inside the third hexagonal enclosure. The present invention has the ability to form multiple longitudinal sampling areas on the hexagonal base plate, thereby enabling dynamic sampling according to the sampling time and ensuring that the plant root system is not damaged; in addition, the multi-layer sampling device provided by the present invention is not only simple in structure, but can also be reused multiple times, facilitating the research of scientific researchers.

Description

Technical Field

[0001] The invention relates to the field of plant technology, in particular to a multi-layer sampling device for dynamically studying changes in plant rhizosphere environment. Background Art

[0002] The rhizosphere refers to a microscopic region of soil that differs from the bulk soil in physical, chemical, and biological properties, influenced by plant root activity. The rhizosphere is very small, generally encompassing a few millimeters from the root axis. It exists not only radially from the root surface to the bulk soil, but also longitudinally from the root base to the root tip. Because studying the rhizosphere microdomain under field conditions is difficult, many researchers use custom-made rhizosphere chambers to simulate the rhizosphere and non-rhizosphere soils. Examples include the rhizosphere chambers used by Yao Jianhua et al. in Chinese Journal of Agricultural Sciences, Vol. 43, No. 4, 2010, and by He et al. in Soil Biology & Biochemistry, Vol. 37, 2005. The chamber dimensions are: length × width × height = 150 mm × 140 mm × 230 mm. A 30 μm nylon mesh divides the chamber into three zones: the rhizosphere with a center width of 20 mm and the non-rhizosphere with a width of 60 mm on either side. The growth space of plant roots is: length × width × height = 150mm × 20mm × 230mm. Therefore, its radial growth can only extend in two directions, which does not conform to the actual environment of extending and growing in all directions under field conditions.

[0003] CN103293035A discloses a rhizosphere test device suitable for studying plant rhizosphere microdomains. Although this application can solve the above-mentioned problems, it only has a large sampling area, making it difficult to perform dynamic sampling based on sampling time, which is a limitation. Therefore, a multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment was invented. Summary of the Invention

[0004] In view of the above problems and / or the problems existing in the existing multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment, the present invention is proposed.

[0005] Therefore, the purpose of the present invention is to provide a multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment, which can solve the above-mentioned existing problems.

[0006] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions:

[0007] A multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment, comprising a hexagonal base plate, a first hexagonal enclosure plate integrated on the top of the hexagonal base plate, a second hexagonal enclosure plate integrated on the top of the hexagonal base plate located inside the first hexagonal enclosure plate, a third hexagonal enclosure plate integrated on the top of the hexagonal base plate located inside the second hexagonal enclosure plate, and a fourth hexagonal enclosure plate integrated on the top of the hexagonal base plate located inside the third hexagonal enclosure plate.

[0008] A plurality of first boxes are inserted between the first hexagonal enclosure and the second hexagonal enclosure, a plurality of second boxes are inserted between the second hexagonal enclosure and the third hexagonal enclosure, and a plurality of third boxes are inserted between the third hexagonal enclosure and the fourth hexagonal enclosure.

[0009] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the space between the first hexagonal enclosure and the second hexagonal enclosure is set as a soil lifting cavity, and six groups of first box bodies are inserted into the soil lifting cavity.

[0010] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the space between the second hexagonal enclosure and the third hexagonal enclosure is set as a rhizosphere cavity, and six groups of second box bodies are inserted into the rhizosphere cavity.

[0011] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the space between the third hexagonal enclosure and the fourth hexagonal enclosure is set as a rhizosphere cavity, and six groups of third boxes are inserted into the rhizosphere cavity.

[0012] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the inner space of the fourth hexagonal enclosure is set as a planting cavity for planting plants.

[0013] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, six groups of first through holes are provided on the second hexagonal enclosure, six groups of second through holes are provided on the third hexagonal enclosure, and six groups of third through holes are provided on the fourth hexagonal enclosure.

[0014] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, each group of the first box bodies is provided with a fourth through hole on the inner side, each group of the second box bodies is provided with a fifth through hole on the outer side, each group of the second box bodies is provided with a sixth through hole on the inner side, each group of the third box bodies is provided with a seventh through hole on the outer side, and each group of the third box bodies is provided with an eighth through hole on the inner side.

[0015] As a preferred embodiment of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment according to the present invention, the fourth through hole is aligned with the first through hole, the first through hole is aligned with the fifth through hole, the fifth through hole is aligned with the sixth through hole, the sixth through hole is aligned with the second through hole, the second through hole is aligned with the seventh through hole, the seventh through hole is aligned with the eighth through hole, and the eighth through hole is aligned with the third through hole;

[0016] A spacing membrane is provided in each of the first through hole, the second through hole, the third through hole, the fourth through hole, the fifth through hole, the sixth through hole, the seventh through hole and the eighth through hole, and the spacing membrane is a 30-micron nylon membrane.

[0017] As a preferred embodiment of the multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to the present invention, it further comprises:

[0018] A lifting assembly is provided to facilitate lifting out the first box body, the second box body and the third box body, and the inner surfaces of the first box body, the second box body and the third box body are all provided with a lifting assembly.

[0019] As a preferred embodiment of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment according to the present invention, the pulling component includes:

[0020] Grooves, the top ends of the inner surfaces of the first box body, the second box body and the third box body are all provided with grooves;

[0021] Support blocks are fixedly installed on both sides of the top end of the inner cavity of the groove;

[0022] A rotating shaft, the rotating shaft being rotatably connected to the support block via a bearing;

[0023] A square plate, the square plate being fixedly installed between the two sets of rotating shafts;

[0024] A torsion spring, wherein the torsion spring is sleeved on the rotating shaft, and the two ends of the torsion spring are fixedly connected to the support block and the square plate respectively;

[0025] A notch is provided on the top of the inner surface of each of the first box body, the second box body and the third box body. The notch is located directly below the groove, and the notch and the groove are connected.

[0026] Compared with existing technologies:

[0027] By sequentially arranging a first hexagonal enclosure, a second hexagonal enclosure, a third hexagonal enclosure and a fourth hexagonal enclosure on the top of the hexagonal base plate, and by inserting a plurality of first boxes, second boxes and third boxes in the space between the first hexagonal enclosure, the second hexagonal enclosure, the third hexagonal enclosure and the fourth hexagonal enclosure plate, it is possible to form multiple longitudinal sampling areas on the hexagonal base plate, thereby enabling dynamic sampling according to the sampling time and ensuring that the plant roots are not damaged; in addition, the multi-layer sampling device provided by the present invention not only has a simple structure, but can also be reused multiple times, which facilitates the research of scientific researchers. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the structure of the present invention;

[0029] Figure 2 It is a schematic top view of the structure of the present invention;

[0030] Figure 3 It is a schematic top view and cross-sectional view of the structure of the present invention;

[0031] Figure 4 This is a schematic diagram of a top view of a local structure of the present invention.

[0032] Figure 5 It is a front view schematic diagram of the structure of the present invention;

[0033] Figure 6 This is a schematic top view of the lifting assembly structure of the present invention;

[0034] Figure 7 For the present invention Figure 6 A schematic diagram of the structure at center A;

[0035] Figure 8 It is a front view schematic diagram of the lifting assembly structure of the present invention.

[0036] In the figure: hexagonal base plate 10, first hexagonal enclosure 20, second hexagonal enclosure 30, first through hole 31, third hexagonal enclosure 40, second through hole 41, fourth hexagonal enclosure 50, third through hole 51, first box body 60, fourth through hole 61, second box body 70, fifth through hole 71, sixth through hole 72, third box body 80, seventh through hole 81, eighth through hole 82, groove 90, support block 91, rotating shaft 92, square plate 93, torsion spring 94, notch 95, and spacer membrane 100. DETAILED DESCRIPTION

[0037] To make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. Example

[0038] The present invention provides a multi-layer sampling device for dynamically studying changes in plant rhizosphere environment. Figures 1-8 , comprising a hexagonal base plate 10, a first hexagonal enclosure 20 being integrated on the top of the hexagonal base plate 10, a second hexagonal enclosure 30 being integrated on the top of the hexagonal base plate 10 located inside the first hexagonal enclosure 20, a third hexagonal enclosure 40 being integrated on the top of the hexagonal base plate 10 located inside the second hexagonal enclosure 30, and a fourth hexagonal enclosure 50 being integrated on the top of the hexagonal base plate 10 located inside the third hexagonal enclosure 40;

[0039] A plurality of first boxes 60 are inserted between the first hexagonal enclosing plate 20 and the second hexagonal enclosing plate 30 , a plurality of second boxes 70 are inserted between the second hexagonal enclosing plate 30 and the third hexagonal enclosing plate 40 , and a plurality of third boxes 80 are inserted between the third hexagonal enclosing plate 40 and the fourth hexagonal enclosing plate 50 .

[0040] The space between the first hexagonal enclosure 20 and the second hexagonal enclosure 30 is set as a soil lifting cavity, in which six groups of first box bodies 60 are inserted; the space between the second hexagonal enclosure 30 and the third hexagonal enclosure 40 is set as a root cavity, in which six groups of second box bodies 70 are inserted; the space between the third hexagonal enclosure 40 and the fourth hexagonal enclosure 50 is set as a rhizosphere cavity, in which six groups of third box bodies 80 are inserted; the inner cavity space of the fourth hexagonal enclosure 50 is set as a planting cavity for planting plants.

[0041] The second hexagonal enclosure 30 is provided with six groups of first through holes 31, the third hexagonal enclosure 40 is provided with six groups of second through holes 41, the fourth hexagonal enclosure 50 is provided with six groups of third through holes 51, the inner side of each group of first box bodies 60 is provided with a fourth through hole 61, the outer side of each group of second box bodies 70 is provided with a fifth through hole 71, the inner side of each group of second box bodies 70 is provided with a sixth through hole 72, and the outer side of each group of third box bodies 80 is provided with a The seventh through hole 81, an eighth through hole 82 is opened on the inner side of each group of third box bodies 80, the fourth through hole 61 is aligned with the first through hole 31, the first through hole 31 is aligned with the fifth through hole 71, the fifth through hole 71 is aligned with the sixth through hole 72, the sixth through hole 72 is aligned with the second through hole 41, the second through hole 41 is aligned with the seventh through hole 81, the seventh through hole 81 is aligned with the eighth through hole 82, and the eighth through hole 82 is aligned with the third through hole 51;

[0042] A spacer membrane 100 is provided in each of the first through hole 31 , the second through hole 41 , the third through hole 51 , the fourth through hole 61 , the fifth through hole 71 , the sixth through hole 72 , the seventh through hole 81 and the eighth through hole 82 . The spacer membrane 100 is a 30-micron nylon membrane.

[0043] When used, the specific steps are as follows:

[0044] Step 1: air-dry and sieve the soil sample retrieved from the field, and then place it into the first box body 60, the second box body 70, the third box body 80 and the planting cavity;

[0045] Step 2: Plant the plants in the planting cavity;

[0046] Step 3: During the growth and development of the plant, relevant information or data of the rhizosphere soil and non-rhizosphere soil can be obtained by removing the first box body 60, the second box body 70 or the third box body 80, thereby promoting the normal development of relevant research on the rhizosphere micro-domain. Example

[0047] In order to facilitate lifting out the first box body 60 , the second box body 70 and the third box body 80 , lifting components are provided on the inner surfaces of the first box body 60 , the second box body 70 and the third box body 80 based on Example 1.

[0048] The lifting assembly includes: a groove 90, a support block 91, a rotating shaft 92, a square plate 93, a torsion spring 94, and a notch 95;

[0049] The top of the inner surface of the first box body 60, the second box body 70 and the third box body 80 are all provided with a groove 90, and support blocks 91 are fixedly installed on both sides of the top of the inner cavity of the groove 90. The rotating shaft 92 is rotatably connected to the support block 91 through a bearing. The square plate 93 is fixedly installed between the two sets of rotating shafts 92, and the torsion spring 94 is sleeved on the rotating shaft 92, and the two ends of the torsion spring 94 are fixedly connected to the support block 91 and the square plate 93 respectively. The top of the inner surface of the first box body 60, the second box body 70 and the third box body 80 are all provided with a notch 95, the notch 95 is located directly below the groove 90, and the notch 95 and the groove 90 are connected.

[0050] When used, the specific steps are as follows:

[0051] When the box body needs to be taken out, the square plate 93 is first rotated from the notch 95. At this time, the torsion spring 94 will be deformed until the square plate 93 contacts the top of the groove 90 after rotation. At this time, the square plate 93 and the groove 90 will be in a vertical state. Then, the square plate 93 is pulled upward to be able to lift the box body out. After that, the square plate 93 will be restored to its original position under the action of the torsion spring 94.

[0052] Although the present invention has been described above with reference to embodiments, various modifications may be made thereto and equivalent components may be substituted without departing from the scope of the present invention. In particular, as long as there are no structural conflicts, the various features of the embodiments disclosed herein may be combined with each other in any manner, and the omission of an exhaustive description of such combinations in this specification is solely for the sake of space and resource conservation. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment, characterized in that: The invention comprises a hexagonal bottom plate (10), wherein a first hexagonal enclosure plate (20) is integrally provided on the top of the hexagonal bottom plate (10), a second hexagonal enclosure plate (30) is integrally provided on the top of the hexagonal bottom plate (10) located inside the first hexagonal enclosure plate (20), a third hexagonal enclosure plate (40) is integrally provided on the top of the hexagonal bottom plate (10) located inside the second hexagonal enclosure plate (30), and a fourth hexagonal enclosure plate (50) is integrally provided on the top of the hexagonal bottom plate (10) located inside the third hexagonal enclosure plate (40); A plurality of first boxes (60) are inserted between the first hexagonal enclosure (20) and the second hexagonal enclosure (30), a plurality of second boxes (70) are inserted between the second hexagonal enclosure (30) and the third hexagonal enclosure (40), and a plurality of third boxes (80) are inserted between the third hexagonal enclosure (40) and the fourth hexagonal enclosure (50); The second hexagonal enclosing plate (30) is provided with six groups of first through holes (31), the third hexagonal enclosing plate (40) is provided with six groups of second through holes (41), and the fourth hexagonal enclosing plate (50) is provided with six groups of third through holes (51); Each group of the first box bodies (60) has a fourth through hole (61) on its inner side, each group of the second box bodies (70) has a fifth through hole (71) on its outer side, each group of the second box bodies (70) has a sixth through hole (72) on its inner side, each group of the third box bodies (80) has a seventh through hole (81) on its outer side, and each group of the third box bodies (80) has an eighth through hole (82) on its inner side; The fourth through hole (61) is aligned with the first through hole (31), the first through hole (31) is aligned with the fifth through hole (71), the fifth through hole (71) is aligned with the sixth through hole (72), the sixth through hole (72) is aligned with the second through hole (41), the second through hole (41) is aligned with the seventh through hole (81), the seventh through hole (81) is aligned with the eighth through hole (82), and the eighth through hole (82) is aligned with the third through hole (51); A spacing membrane (100) is provided in each of the first through hole (31), the second through hole (41), the third through hole (51), the fourth through hole (61), the fifth through hole (71), the sixth through hole (72), the seventh through hole (81) and the eighth through hole (82), and the spacing membrane (100) is a nylon membrane with a thickness of 30 microns.

2. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the first hexagonal enclosure plate (20) and the second hexagonal enclosure plate (30) is set as a soil lifting cavity, and six groups of first box bodies (60) are inserted into the soil lifting cavity.

3. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the second hexagonal enclosure plate (30) and the third hexagonal enclosure plate (40) is set as a root cavity, and six groups of second box bodies (70) are inserted into the root cavity.

4. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the third hexagonal enclosure (40) and the fourth hexagonal enclosure (50) is set as a rhizosphere cavity, and six groups of third box bodies (80) are inserted into the rhizosphere cavity.

5. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The inner cavity space of the fourth hexagonal enclosure (50) is set as a planting cavity for planting plants.

6. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: Also includes: A lifting assembly is provided for facilitating lifting the first box body (60), the second box body (70) and the third box body (80), and the inner surfaces of the first box body (60), the second box body (70) and the third box body (80) are all provided with the lifting assembly.

7. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 6, characterized in that: The lifting assembly includes: A groove (90), wherein the top ends of the inner surfaces of the first box body (60), the second box body (70) and the third box body (80) are all provided with a groove (90); Support blocks (91), support blocks (91) are fixedly mounted on both sides of the top end of the inner cavity of the groove (90); A rotating shaft (92), the rotating shaft (92) being rotatably connected to the support block (91) via a bearing; A square plate (93), wherein the square plate (93) is fixedly mounted between the two sets of rotating shafts (92); A torsion spring (94), wherein the torsion spring (94) is sleeved on the rotating shaft (92), and both ends of the torsion spring (94) are fixedly connected to the support block (91) and the square plate (93) respectively; A notch (95) is provided on the top of the inner surface of each of the first box body (60), the second box body (70), and the third box body (80). The notch (95) is located directly below the groove (90), and the notch (95) and the groove (90) are connected.

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