Preparation method of sheep manure bio-organic fertilizer, prepared sheep manure bio-organic fertilizer and application of prepared sheep manure bio-organic fertilizer

Abstract

The invention relates to the field of fertilizers, and discloses a preparation method of a sheep manure bio-organic fertilizer, the prepared sheep manure bio-organic fertilizer and application of the sheep manure bio-organic fertilizer, the preparation method comprises the following steps: mixing a compost raw material and a composite compost microbial inoculum for compost fermentation, and when the internal temperature of a compost fermentation product is reduced to not more than 35 DEG C, carrying out fermentation; the carbon nitrogen ratio of the compost raw materials is (20-25): 1, the composite compost microbial inoculum comprises trichoderma, paenibacillus, bacillus and burkholderia, and the compost raw materials comprise fresh sheep manure, rape seed cakes and straw. The organic fertilizer prepared by the invention is especially suitable for planting mulberries and blackberries.

Description

Technical Field

[0001] The invention relates to the field of materials, and in particular to a method for preparing a sheep dung bio-organic fertilizer, the prepared sheep dung bio-organic fertilizer and applications thereof. Background Art

[0002] With the continuous expansion of agricultural production scale and the improvement of the intensive degree of livestock and poultry breeding in my country, a large amount of agricultural organic solid waste is also generated. Studies have shown that when crop straw is directly returned to the field as a soil conditioner, due to the lack of mature and stable organic matter, it is easy to slow down the mineralization of organic matter and cause an oxygen-deficient environment in the soil, which not only causes a waste of resources, but also aggravates environmental pollution. Rapeseed cake is a by-product obtained after rapeseed is pressed for oil. It is rich in nutrients such as protein and amino acids. If it lacks maturity, it will poison the root system of plants and affect crop growth. In addition, livestock and poultry manure is not effectively treated and utilized. After discharge, it causes eutrophication of water bodies, eventually leading to the death of aquatic organisms and threatening human life. Therefore, the reduction and resource utilization of agricultural organic solid waste is an effective way to reduce environmental pollution. At present, the main way to realize the resource utilization of agricultural waste is to carry out composting. However, the composting process often encounters problems such as low fermentation temperature, long fermentation time, incomplete killing of pathogens and eggs, and loss of nutrients due to insufficient fermentation. Therefore, promoting compost maturity and shortening the composting fermentation cycle are the keys to improving composting quality and efficiency. Rapeseed cake, sheep manure and straw are the main agricultural by-products or wastes in some areas. At present, there are few reports on the mixed composting of these three solid wastes. Summary of the invention

[0003] In order to overcome the above technical problems, a method for preparing a sheep manure bio-organic fertilizer and the prepared sheep manure bio-organic fertilizer are provided. The organic fertilizer has comprehensive nutritional components and solves the effective treatment of three solid wastes at one time.

[0004] In order to achieve the above-mentioned object, the first aspect of the present invention provides a method for preparing sheep manure biological organic fertilizer, wherein the preparation method comprises: mixing a compost raw material and a composite composting bacterial agent to obtain a compost fermentation product, and then performing composting fermentation, and when the internal temperature of the compost fermentation product drops to no more than 35°C, the composting fermentation is terminated to obtain sheep manure organic fertilizer, wherein the compost raw material comprises fresh sheep manure, rapeseed cake and straw, and the carbon-nitrogen ratio thereof is 20-25:1, and the composite composting bacterial agent comprises Trichoderma, Paenibacillus, Bacillus and Burkholderia.

[0005] The above technical solution, by using a composite composting agent and controlling the carbon-nitrogen ratio of the composting raw materials, is conducive to promoting compost maturity and shortening the composting cycle, thereby improving the quality and efficiency of composting. In addition, the above solution uses fresh sheep manure, rapeseed cake and straw as raw materials, solving the treatment of three types of waste solids at one time, turning waste into treasure, and is suitable for market promotion and application.

[0006] The present invention has no special requirements for the method of ending the compost fermentation, as long as it can prevent further fermentation, such as removing part of the moisture of the compost fermentation product to end the fermentation. More specifically, it can be achieved by mechanical dehydration or air drying so that the moisture content of the compost fermentation product does not exceed 30%.

[0007] In some embodiments, preferably, the moisture content of the compost raw material is 50-60%. The moisture content of the compost raw material can be 50%, 53%, 55%, 57%, 60%, etc.

[0008] In some embodiments, preferably, the compost fermentation time is 30-40 days. The method provided by the present invention has a shorter fermentation time, generally about 30 days, and the fermentation time can also be 35 days, 40 days, etc.

[0009] In some embodiments, preferably, the mass ratio of the fresh sheep manure to the rapeseed cake is 1:1, and the amount of the straw is adjusted so that the carbon-nitrogen ratio of the compost raw material is 20-25:1. The carbon-nitrogen ratio can be 20:1, 22:1, 25:1, etc. In order to adjust the appropriate carbon-nitrogen ratio, urea can be added if necessary.

[0010] In some embodiments, preferably, based on the dry matter of the composting raw materials, the composite composting bacterial agent accounts for 4-10wt% (such as 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%) of the composting raw materials.

[0011] In some embodiments, preferably, the Trichoderma is Trichoderma asperellum TC01 (CGMCC No. 3.19218), the Paenibacillus is Paenibacillus taichungensis 80533, the Bacillus is Bacillus cereus 932875, and the Burkholderia is Burkholderia cepacia 932855. The present invention has no particular requirements for the specific types of Paenibacillus, Bacillus and Burkholderia, and any commercially available or currently preserved ones are all acceptable. For example, Paenibacillus can be a bacterium with a preservation number of CGMCC NO. 25184, Bacillus can be a bacterium with a preservation number of CGMCC No. 1.15914, and Burkholderia can be a bacterium with a preservation number of CGMCC No. 1.2787.

[0012] In some embodiments, preferably, the mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia is 1-3:1:1:3-5. The mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia can be 1:1:1:3, 1:1:1:4, 1:1:1:5, 2:1:1:3, 2:1:1:4, 2:1:1:5, 3:1:1:3, 3:1:1:4, 3:1:1:5, etc.

[0013] In some embodiments, preferably, the compost raw material also includes kitchen waste, and the kitchen waste is preferably kitchen waste from hot pot restaurants. The present invention also unexpectedly has a good decomposition effect on kitchen waste, especially for kitchen waste from hot pot restaurants, which contains more grease, especially butter grease. This type of kitchen waste is generally difficult to handle, especially for some areas where people like to eat hot pot, the treatment of this type of kitchen waste becomes an urgent problem to be solved. Through the method of the present invention, waste can be quickly and easily turned into treasure, and the decomposition rate of grease in the method provided by the present invention can reach up to about 80%.

[0014] In some embodiments, preferably, based on the total amount of the fresh sheep manure and rapeseed cake, the amount of the kitchen waste is 10-20wt%. The amount of the kitchen waste can be 10wt%, 12wt%, 15wt%, 17wt%, 20wt%, etc.

[0015] In some embodiments, preferably, the mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia is 3:1:1:3-5. When the composting raw material includes kitchen waste, especially kitchen waste from hot pot restaurants, using the ratio of bacterial agents within this range can improve the decomposition rate of oil.

[0016] In some embodiments, preferably, the composting fermentation process includes: mixing the compost raw material and the composite composting agent and then directly composting and fermenting, and turning the compost every 6-12 hours during the composting temperature rise period, every 2-3 days during the high temperature period, and every 4-5 days during the temperature drop period. The present invention unexpectedly found that the provided composite composting agent can be directly mixed with the compost raw material without activation and then fermented, and accordingly the number of compost turnings can be increased during the composting process. The method of the present invention is very friendly to some areas or operators who do not have the ability to activate the agent before using it.

[0017] In some embodiments, preferably, the composting fermentation process includes: activating the composite composting agent and then mixing it with the compost raw material and then composting and fermenting, and turning the compost once every 5 days during the composting temperature rise period and high temperature period, and turning the compost once every 7 days during the temperature drop and maturity period;

[0018] The method for activating the composite composting agent comprises: inoculating the composite composting agent into a mixed fermentation medium, culturing for 30-40 hours at a temperature of 28-30° C., a pH of 7.0-7.2, and a rotation speed of 180 r / min;

[0019] The mixed fermentation medium comprises: 10-20 g / L of soy peptone, 5-10 g / L of corn flour, 80-100 g / L of honey, 5-15 g / L of soybean meal and 0.5-1.5 g / L of chloride salt, and the balance is water.

[0020] Generally speaking, when using a microbial agent, it needs to be activated before use to improve the efficiency of the microbial agent. The conventional activation method is to inoculate each type of bacteria in the culture medium most suitable for its production, and then inoculate each activated bacteria in a mixed fermentation medium for mass culture. After proliferation culture, the activated bacteria are then inoculated into the compost raw material to be processed. The method provided by the present invention optimizes the mixed fermentation medium so that each type of bacteria can be exempted from the activation step of the special culture medium, and directly inoculated into the mixed fermentation medium, and then the cultured composite bacteria are inoculated into the compost raw material to be processed. At this time, the number of compost turning during the compost fermentation period can be appropriately reduced.

[0021] In addition, the present invention unexpectedly discovered that the composite bacterial agent of the present invention can be directly mixed with compost raw materials for fermentation without activation and value increase. When this method is adopted, it is only necessary to increase the frequency of turning the compost during the temperature rise period.

[0022] The second aspect of the present invention provides a sheep manure bio-organic fertilizer, wherein the sheep manure bio-organic fertilizer is prepared according to the preparation method described in the first aspect of the present invention.

[0023] The third aspect of the present invention provides an application of the sheep manure biological organic fertilizer described in the second aspect of the present invention in fruit tree planting and tea tree cultivation.

[0024] In some embodiments, preferably, the fruit trees include mulberry trees and blackberry trees.

[0025] In some embodiments, preferably, the application method includes: spreading the sheep manure bio-organic fertilizer at 500-1000 kg / mu during the germination period of the fruit trees, spreading the sheep manure bio-organic fertilizer at 1000-1500 kg / mu during the bud period of the fruit trees, and spreading the sheep manure bio-organic fertilizer at 800-1500 kg / mu during the fruit setting period of the fruit trees.

[0026] According to the method provided by the present invention, the organic fertilizer prepared by the present invention is applied as required in the germination period, bud period and fruit setting period of fruit trees, the yield of fruit trees can be effectively improved, and the economic benefits of fruit trees are greatly improved because of the use of organic fertilizer. The amount of fertilizer applied in the germination period can be 500kg / mu, 600kg / mu, 700kg / mu, 800kg / mu, 900kg / mu, 1000kg / mu, etc., the amount of fertilizer applied in the bud period can be 1000kg / mu, 1100kg / mu, 1200kg / mu, 1300kg / mu, 1400kg / mu, 1500kg / mu, etc., and the amount of fertilizer applied in the fruit setting period can be 800kg / mu, 900kg / mu, 1000kg / mu, 1200kg / mu, 1500kg / mu, etc. The organic fertilizer provided by the present invention is particularly suitable for the planting of mulberries and blackberries. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a temperature trend chart during the fermentation process;

[0028] Figure 2 It is a trend chart of organic matter content during fermentation;

[0029] Figure 3 It is the trend chart of total nitrogen content during fermentation;

[0030] Figure 4 NH during fermentation 4+ Content change trend chart. DETAILED DESCRIPTION

[0031] The specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention. The terms "first", "second", etc. are used to distinguish similar objects, and are not used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of the present application can be implemented in an order other than those illustrated or described here, and the objects distinguished by "first", "second", etc. are generally a class, and the number of objects is not limited. For example, the first object can be one or more. In addition, the term "and / or" in the specification and claims is used to describe the association relationship of associated objects, indicating that there can be three relationships, for example, A and / or B can be represented: A exists alone, A and B exist at the same time, and B exists alone. The character " / " generally indicates that the front and back associated objects are an "or" relationship. In the description of the present application, unless otherwise specified, the meaning of "multiple" is two or more.

[0032] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0033] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0034] The raw materials used in the following examples and comparative examples, unless otherwise specified, are commercially available products or existing preserved strains well known in the art.

[0035] Preparation Example I-1

[0036] Prepare Trichoderma asperellum TC01 (CGMCC No.3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875, and Burkholderia cepacia 932855 in a ratio of 1:1:1:3 for use;

[0037] Prepare a mixed fermentation medium composed of 15 g / L soy peptone, 8 g / L corn flour, 90 g / L honey, 10 g / L soybean meal and 1 g / L chloride salt, with the balance being water.

[0038] The prepared bacterial agents were simultaneously inoculated into the mixed fermentation medium, and cultured for 35 hours at 29° C., pH 7.0, and a rotation speed of 180 r / min to obtain a composite composting bacterial agent.

[0039] Preparation Example I-2

[0040] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Trichoderma asperellum TC01 (CGMCC No. 3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875 and Burkholderia cepacia 932855 was 3:1:1:4.

[0041] Preparation Example I-3

[0042] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Trichoderma asperellum TC01 (CGMCC No. 3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875 and Burkholderia cepacia 932855 was 3:1:1:3.

[0043] Preparation Example I-4

[0044] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Trichoderma asperellum TC01 (CGMCC No. 3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875 and Burkholderia cepacia 932855 was 3:1:1:5.

[0045] Preparation Example I-5

[0046] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Trichoderma asperellum TC01 (CGMCC No. 3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875 and Burkholderia cepacia 932855 was 2:1:1:5.

[0047] Preparation Example I-6

[0048] The preparation was carried out in the manner of Preparation Example Ⅰ-1, with the only difference being that the composition of the mixed fermentation medium was: 10 g / L soy peptone, 10 g / L corn flour, 80 g / L honey, 5 g / L soybean meal and 1.5 g / L chloride salt, with the remainder being water.

[0049] Preparation Example I-7

[0050] The preparation was carried out in the manner of Preparation Example Ⅰ-1, with the only difference being that the composition of the mixed fermentation medium was: 20 g / L soy peptone, 5 g / L corn flour, 100 g / L honey, 15 g / L soybean meal and 0.5 g / L chloride salt, with the remainder being water.

[0051] Preparation Example I-8

[0052] The preparation was carried out in the manner of Preparation Example Ⅰ-1, with the only difference being that the composition of the mixed fermentation medium was: 5 g / L soy peptone, 8 g / L corn flour, 50 g / L honey, 8 g / L soybean meal and 0.7 g / L chloride salt, with the remainder being water.

[0053] Preparation Example I-9

[0054] Trichoderma asperellum TC01 (CGMCC No.3.19218), Paenibacillus taichungensis 80533, Bacillus cereus 932875, and Burkholderia cepacia 932855 were mixed in a ratio of 1:1:1:3 to obtain a composite composting agent. The above strains were all stored in glycerol at -80°C before use.

[0055] Preparation Example I-10

[0056] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Paenibacillus taichungensis 80533, Bacillus cereus 932875 and Burkholderia cepacia 932855 was 1:1:4.

[0057] Preparation Example I-11

[0058] The preparation was carried out in the same manner as in Preparation Example I-1, except that the mass ratio of Trichoderma asperellum TC01 (CGMCC No. 3.19218), Paenibacillus taichungensis 80533 and Bacillus cereus 932875 was 3:1:1.

[0059] Preparation Example II-1

[0060] Compost raw materials were prepared according to a carbon-nitrogen ratio of 23:1. The composition of the compost raw materials was as follows: by mass ratio, fresh sheep manure: rapeseed cake: straw: kitchen waste from hot pot restaurants = 2:2:3:0.6. The moisture content of the compost raw materials was 56%. Based on the dry matter of the compost raw materials, 6 wt% of the composite composting agent from Preparation Example Ⅰ-2 was added, and then composting was carried out. The initial size of each pile was approximately 2.5 m long × 2 m wide × 0.75 m high. During the composting process, natural ventilation and turning were used to meet the needs of aerobic composting. The compost was turned once every 5 days during the composting heating period and high temperature period, and once every 7 days during the cooling and maturation period. The composting fermentation was terminated when the internal temperature of the pile did not exceed 35°C. The total fermentation days were 30 days.

[0061] Preparation Example II-2

[0062] The preparation was carried out in the manner of Preparation Example Ⅱ-1, except that the carbon-nitrogen ratio of the composting raw material was 20:1, the ratio of fresh sheep manure: rapeseed cake: straw: kitchen waste from a hot pot restaurant was 2:2:2.8:0.4, the moisture content of the composting raw material was 52%, and the amount of the composite composting agent from Preparation Example Ⅰ-2 added was 5wt%, based on the dry matter of the composting raw material.

[0063] Preparation Example II-3

[0064] The preparation was carried out in the manner of Preparation Example Ⅱ-1, except that the carbon-nitrogen ratio of the composting raw material was 25:1, fresh sheep manure: rapeseed cake: straw: hot pot restaurant kitchen waste = 2:2:3.3:0.8, the moisture content of the composting raw material was 57%, and the amount of the composite composting agent from Preparation Example Ⅰ-2 added was 10wt% based on the dry matter of the composting raw material.

[0065] Preparation Examples II-4 and 5

[0066] The preparation was carried out in the same manner as in Preparation Example II-1, with the only difference being that the composite composting bacterial agent came from Preparation Example I-3 and Preparation Example I-4 respectively.

[0067] Preparation Example II-6

[0068] The preparation was carried out in the same manner as in Preparation Example II-1, with the only difference being that the composite composting agent came from Preparation Example I-1.

[0069] Preparation Examples II-7 to 13

[0070] The preparation was carried out in the same manner as in Preparation Example II-1, with the only difference being that the composite composting bacterial agent was from Preparation Examples I-5 to 11 in sequence.

[0071] Among them, the composite composting agent of Preparation Example 11 comes from Preparation Example I-9. The compost is turned every 8 hours during the composting temperature rise period, every 2 days during the high temperature period, and every 5 days during the temperature drop and maturity period.

[0072] Preparation Example II-14

[0073] The method of Preparation Example II-1 was followed, except that 1 wt% of the composite composting agent from Preparation Example I-2 was added.

[0074] Preparation Example II-15

[0075] The method of Preparation Example II-1 is followed, except that the composting raw materials consist of fresh pig manure, rapeseed cake and straw.

[0076] Preparation Example II-16

[0077] The preparation was carried out in the same manner as in Preparation Example II-1, with the only difference being that: fresh sheep manure: rapeseed cake: straw: kitchen waste from a hot pot restaurant = 2:2:3:1.2.

[0078] Comparative Preparation Example 1

[0079] The preparation was carried out in the same manner as in Preparation Example II-1, except that no composite composting agent was added and only an equal amount of sterile water was added.

[0080] Comparative Preparation Example 2

[0081] The method of Preparation Example II-1 was followed, except that the carbon-nitrogen ratio of the compost raw material was 40:1.

[0082] Comparative Preparation Example 3

[0083] The method of Preparation Example II-1 was followed, except that the carbon-nitrogen ratio of the compost raw material was 10:1.

[0084] The composting process of Preparation Example II-1 and the composting process of Comparative Preparation Example 1 were tracked and monitored, and the monitoring items were temperature, organic matter content (OM), total nitrogen, NH 4+ , the results are as follows:

[0085] Both of them began to enter high temperature (>50°C) on the fourth day. The highest temperature of Preparation Example II-1 was 63°C, while the highest temperature of Comparative Preparation Example 1 was 61°C. The high temperature period of the two was maintained for 13 days and 12 days respectively. The temperature changes of the two during the composting process are as follows: Figure 1 shown.

[0086] At the end of composting, the OM content of the two was significantly different. The OM content of the organic fertilizer prepared in Preparation Example II-1 was significantly higher than that of the comparative example 1. Figure 2 shown.

[0087] At the end of composting, there was no significant difference in total nitrogen and total carbon content between the two, but NH 4+ The content varies significantly, such as Figure 3 The total nitrogen content of the two Figure 4 Shows the NH of both 4+ content.

[0088] After the fermentation of Preparation Examples II-1 to II-16 and Comparative Preparation Examples 1-3, water-soluble carbon, total nitrogen, NH 4+ The specific values ​​of fat decomposition rate, effective viable bacteria count, moisture content, ascaris egg mortality rate, fecal coliform count, etc. are shown in Table 1.

[0089] Table 1

[0090]

[0091]

[0092] It can be seen from Table 1 that, in combination with Preparation Examples II-1 to 5 and Preparation Example II-6, when the mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia is in the range of 3:1:1:3-5, the water-soluble carbon, total nitrogen, and NH 4+and organic matter content are higher, and the decomposition rate of fat is also higher; combined with Preparation Example Ⅱ-1 and Preparation Example Ⅱ-11, it can be seen that after inoculation of the mixed bacterial agent, the fermentation is carried out directly without activation and value-added. Although the application value of the organic fertilizer and the decomposition rate of fat are slightly lower than those of Preparation Example Ⅱ-1, generally speaking, a good fermentation effect is still obtained; combined with Preparation Example Ⅱ-1 and Preparation Example Ⅱ-14 and Preparation Example Ⅱ-15, it can be seen that when the dose of the inoculated composite bacteria is sufficient, a better fermentation effect can be obtained, and the preparation method provided by the present invention is more suitable for raw materials containing fresh sheep manure than raw materials containing fresh pig manure; combined with Preparation Example Ⅱ-1 and Preparation Example Ⅱ-16, it can be seen that controlling the kitchen waste of the hot pot restaurant within an appropriate range can make the decomposition efficiency of fat higher; combined with Preparation Example Ⅱ-1 and Preparation Examples Ⅱ-12 and Ⅱ-13, it can be seen that when the corresponding fungi are lacking in the composite bacterial agent, its decomposition rate of fat is significantly reduced.

[0093] Examples 1-16

[0094] During the germination period of mulberry trees (Yuezhen Da 10), 800 kg / mu of organic fertilizers prepared from Preparation Examples Ⅱ-1 to Ⅱ-16 were applied respectively, and during the bud period, 1200 kg / mu of organic fertilizers prepared from Preparation Examples Ⅱ-1 to Ⅱ-16 were applied respectively, and further during the fruit setting period of mulberry trees, 1000 kg / mu of organic fertilizers prepared from Preparation Examples Ⅱ-1 to Ⅱ-16 were applied respectively.

[0095] Embodiment 17

[0096] During the germination period, bud period and fruit setting period of blackberry trees (Shani), the organic fertilizer from Preparation Example II-1 was applied at 800 kg / mu, 1200 kg / mu and 1000 kg / mu respectively.

[0097] Embodiment 18

[0098] The procedure of Example 17 was followed, except that the blackberry trees were replaced with apple trees (Lu Li).

[0099] Embodiment 19

[0100] The procedure of Example 17 was followed, except that the blackberry trees were replaced with grape trees (Kyoho).

[0101] Embodiment 20

[0102] The procedure of Example 17 was followed, except that the blackberry trees were replaced with cherry trees (Black Pearl).

[0103] Comparative Examples 1-3

[0104] The method of Example 1 was followed, except that the organic fertilizers applied in Comparative Examples 1-3 were from Comparative Example Preparation Examples 1-3, respectively.

[0105] Comparative Example 4

[0106] The method of Example 1 is followed, except that the fertilizer applied is ordinary farmyard manure.

[0107] Comparative Example 5

[0108] The method of Example 17 was followed, except that the fertilizer applied was ordinary farmyard manure.

[0109] Comparative Examples 6-8

[0110] The method of Comparative Example 4 was followed, except that the fruit trees were apple trees (Luli), grape trees (Kyoho) and cherry trees (Black Pearl).

[0111] Table 2

[0112]

[0113]

[0114] In the above test results, the single fruit weight and sugar content were measured by randomly selecting 10 fruit trees for testing and taking the average value.

[0115] It can be seen that compared with ordinary farmyard manure and fertilizers fermented without adding composite microbial agents, the organic fertilizer prepared by the present invention can significantly increase the per-acre yield and single fruit weight of mulberries and blackberries, while the organic fertilizer provided by the present invention has no significant improvement on the yield and single fruit weight of apples, grapes and cherries compared with ordinary farmyard manure. At the same time, it can be seen from comparative examples 2 and 3 and example 1 that controlling the carbon-nitrogen ratio during fermentation has a great influence on the comprehensive nutritional components of the prepared organic fertilizer, thereby affecting the yield of fruit trees in the later stage.

[0116] Combining Comparative Example 4 with Example 1, and Comparative Example 5 with Example 1, it can be seen that applying the organic fertilizer provided by the present invention can significantly increase the yield, single fruit weight and sugar content of mulberries and blackberries. Combining Examples 18-20 and Comparative Examples 6-8, it can be seen that although the organic fertilizer of the present invention increases the yield of apples, grapes and cherries, the increase is not significant. It can be seen that the organic fertilizer prepared by the present invention is particularly suitable for planting mulberries and blackberries, which is beneficial to further improve their economic value.

[0117] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various specific technical features in any appropriate manner. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations. However, these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A method for preparing sheep dung bio-organic fertilizer, characterized in that: The preparation method comprises: mixing compost raw materials and a composite composting agent to obtain a compost fermentation product, then performing composting fermentation, and when the internal temperature of the compost fermentation product drops to no more than 35° C., the composting fermentation is terminated to obtain sheep manure organic fertilizer, wherein the compost raw materials comprise fresh sheep manure, rapeseed cake and straw, and the carbon-nitrogen ratio thereof is 20-25:1, and the composite composting agent comprises Trichoderma, Paenibacillus, Bacillus and Burkholderia.

2. The preparation method according to claim 1, wherein The moisture content of the compost raw material is 50-60%; And / or, the composting fermentation time is 30-40 days.

3. The preparation method according to claim 1 or 2, wherein The mass ratio of the fresh sheep manure to the rapeseed cake is 1:1, and the amount of the straw is adjusted so that the carbon-nitrogen ratio of the compost raw material is 20-25:

1.

4. The preparation method according to any one of claims 1 to 3, wherein The composite composting agent accounts for 4-10wt% of the composting raw material based on the dry matter of the composting raw material; and / or, the Trichoderma is Trichoderma asperellum TC01 (CGMCC No. 3.19218), the Paenibacillus is Paenibacillus taichungensis 80533, the Bacillus is Bacillus cereus 932875, and the Burkholderia is Burkholderia cepacia 932855; Preferably, the mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia is 1-3:1:1:3-5.

5. The preparation method according to claim 4, wherein: The compost raw materials also include kitchen waste, and the kitchen waste is preferably kitchen waste from hot pot restaurants; Preferably, based on the total amount of the fresh sheep manure and rapeseed cake, the amount of the kitchen waste is 10-20wt%; Preferably, the mass ratio of Trichoderma, Paenibacillus, Bacillus and Burkholderia is 3:1:1:3-5.

6. The preparation method according to any one of claims 1 to 5, wherein: The composting fermentation process comprises: mixing the compost raw material and the composite composting agent and then directly composting and fermenting, and turning the compost every 6-12 hours during the composting temperature rising period, every 2-3 days during the high temperature period, and every 4-5 days during the temperature falling and mature period; Or, the composting fermentation process includes: activating the composite composting agent and then mixing it with the compost raw material and then composting and fermenting, and turning the compost once every 5 days during the composting temperature rise period and high temperature period, and turning the compost once every 7 days during the temperature drop and mature period; The method for activating the composite composting agent comprises: inoculating the composite composting agent into a mixed fermentation medium, culturing for 30-40 hours at a temperature of 28-30° C., a pH of 7.0-7.2, and a rotation speed of 180 r / min; The mixed fermentation medium comprises: 10-20 g / L of soy peptone, 5-10 g / L of corn flour, 80-100 g / L of honey, 5-15 g / L of soybean meal and 0.5-1.5 g / L of chloride salt, and the balance is water.

7. A sheep manure biological organic fertilizer, characterized in that: Prepared according to the preparation method described in any one of claims 1 to 6.

8. Use of the sheep manure bio-organic fertilizer according to claim 7 in fruit tree planting and tea tree cultivation.

9. The use according to claim 8, wherein: The fruit trees include mulberry trees and blackberry trees.

10. The use according to claim 8 or 9, wherein: The application method comprises: applying the sheep manure bio-organic fertilizer at 500-1000 kg / mu during the germination period of the fruit trees, applying the sheep manure bio-organic fertilizer at 1000-1500 kg / mu during the bud period of the fruit trees, and applying the sheep manure bio-organic fertilizer at 800-1500 kg / mu during the fruit setting period of the fruit trees.

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