A magnetic bead method for blowing and aspirating nucleic acid extraction reagent strip and silicon-based chip amplification method

Abstract

The invention discloses a magnetic bead method blowing and aspirating nucleic acid extraction reagent strip, comprising a plate base, a barrel-type pipette tip chamber I, a barrel-type pipette tip chamber II, a barrel-type pipette tip chamber III, a lysis and binding liquid chamber, a cleaning liquid chamber I, a cleaning liquid chamber II, a magnetic bead storage chamber, an elution liquid chamber, a UV glue chamber, and a silicon-based amplification detection chip arranged on the plate base. The plastic injection moldings involved in the invention are all completed by an injection molding machine, and are light in weight, simple to make, and can be produced with one mold and multiple holes. The cost price and the overall price of a single tube are about 1.5 yuan cheaper, saving consumables costs. The embedded reagents can be batch canned by a linked peristaltic pump, saving labor and production equipment and site costs. The advantages of silicon-based chips, silicon-based materials will not cause immune reactions, have good biocompatibility, and the consistency of experimental results is more stable than plastic and glass-based, and are suitable for detecting DNA, RNA cell pathology, protein enzymolysis, virus and bacterial diagnosis.

Description

Technical Field

[0001] The invention relates to the technical field of nucleic acid extraction and PCR amplification, and in particular to a magnetic bead method for blowing and aspirating nucleic acid extraction reagent strips and a silicon-based chip amplification method. Background Art

[0002] The magnetic bead-based nucleic acid extraction and PCR amplification currently used in the market have some problems to varying degrees: the existing nucleic acid extraction is mostly a 96-well deep-well nucleic acid extraction plate, which cannot achieve single-person nucleic acid extraction and is not convenient enough. The existing nucleic acid extraction can only extract but cannot achieve rapid amplification. The magnetic bead-based blowing and suction nucleic acid extraction reagent strip and silicon-based chip amplification solution can extract and rapidly amplify nucleic acid from a single person and a single sample. The existing chip amplification is made of plastic injection molding and cannot meet the requirements of rapid heating and cooling rates.

[0003] 1. The artificial magnetic bead method has low efficiency and slow speed in nucleic acid extraction, and the extraction results vary from person to person and are unstable.

[0004] 2. Semi-automatic magnetic bead method for nucleic acid extraction requires manual nucleic acid transfer after extraction, resulting in high testing consumables and labor costs, inconsistent and unstable pipetting accuracy.

[0005] 3. The fully automatic magnetic bead method for nucleic acid extraction has high instrument costs and special consumables. In addition, it is limited by the environment, personnel, and technology and is not flexible enough.

[0006] 4. Currently, most PCR amplifications on the market use eight-tube and plastic chip amplification. Due to the material of the amplifications, the temperature cannot be quickly raised or lowered for PCR amplification, and therefore cannot meet the current demand for rapid testing. Summary of the invention

[0007] The purpose of the present invention is to provide a magnetic bead method blowing and aspirating nucleic acid extraction reagent strip and a silicon-based chip amplification method to solve the problems of high cost of nucleic acid extraction reagent strips and low amplification efficiency of silicon-based chips.

[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0009] The present invention provides a magnetic bead method blowing and aspirating nucleic acid extraction reagent strip, comprising a plate base, a barrel-type pipette tip chamber I, a barrel-type pipette tip chamber II, a barrel-type pipette tip chamber III, a lysis and binding liquid chamber, a cleaning liquid chamber I, a cleaning liquid chamber II, a magnetic bead storage chamber, an elution liquid chamber, a UV glue chamber, and a silicon-based amplification detection chip arranged on the plate base in sequence;

[0010] A pipette tip I is adapted to be arranged in the barrel-type pipette tip chamber I, a pipette tip II is adapted to be arranged in the barrel-type pipette tip chamber II, a pipette tip III is adapted to be arranged in the barrel-type pipette tip chamber III, and an amplification cavity corresponding to the silicon-based amplification detection chip is arranged on the plate base.

[0011] Furthermore, the cylindrical pipette tip chamber I, the cylindrical pipette tip chamber II, and the cylindrical pipette tip chamber III are all cylindrical flat-bottom chambers.

[0012] Furthermore, the pipette tip I is a pipette tip with a volume of 1000 μL, and the pipette tip and the pipette tip III are both pipette tips with a volume of 100 μL.

[0013] Furthermore, the lysis and binding liquid chamber is a cylindrical round-bottom liquid chamber with a volume of 3 ml; 500 μl of lysis and binding liquid is injected into it; and a heating module is arranged at the bottom of the lysis and binding liquid chamber.

[0014] Furthermore, the cleaning liquid chamber I and the cleaning liquid chamber II are both round-bottom chambers with a volume of 1.5 ml, and 250-300 μl of cleaning liquid is added to each of them.

[0015] Furthermore, the magnetic bead storage chamber is a cylindrical round-bottom chamber with a volume of 2 ml, and 80 microliters of magnetic beads are added therein; the elution liquid chamber is a cylindrical round-bottom chamber with a volume of 2 ml, and 100 microliters of elution liquid are added therein; the UV glue chamber is a cylindrical round-bottom chamber with a volume of 2 ml, and 300 microliters of UV glue are added therein.

[0016] Furthermore, the silicon-based chip amplification chamber includes four chambers concentrated on one estimation chip.

[0017] Furthermore, the cylindrical pipette tip chamber I, cylindrical pipette tip chamber II, cylindrical pipette tip chamber III, lysis and binding liquid chamber, cleaning liquid chamber I, cleaning liquid chamber II, magnetic bead storage chamber, elution liquid chamber, and UV glue chamber on the plate base are integrally formed on the plate base and formed by injection molding.

[0018] This embodiment discloses a silicon-based chip amplification method, comprising the following steps:

[0019] S1, collect 200 μl of the sample and add it into the lysis and binding liquid chamber, and heat it to 75°C through the bottom heating module;

[0020] S2, taking out the pipette tip I, and repeatedly blowing and sucking the magnetic beads in the magnetic bead storage chamber to mix, then sucking into the pipette tip I, the external magnet of the pipette tip I adsorbs the magnetic beads inside the pipette tip I on the tube wall of the pipette tip I, and the magnetic bead diluent is released and dripped into the magnetic bead storage chamber;

[0021] S3, the pipette tip I transfers the magnetic beads to the top of the lysis and binding liquid chamber, the magnet outside the pipette tip I leaves, the pipette tip I moves downward to suck the lysis and binding liquid in the lysis and binding liquid chamber, and repeatedly blows and sucks to mix, and all the liquid and magnetic beads in the lysis and binding liquid chamber are sucked into the pipette tip I, the pipette tip I rises to a specified height, the external magnet of the pipette tip I adsorbs the magnetic beads in the pipette tip I on the inner wall of the pipette tip I, the pipette tip I releases the lysis and binding liquid in it and drops it into the lysis and binding liquid chamber, and the magnetic beads adsorbed with the sample nucleic acid are retained in the pipette tip I;

[0022] The pipette tip I is transferred to the top of the cleaning liquid chamber I, the outer magnet of the pipette tip I leaves the pipette tip I, the pipette tip I descends into the cleaning liquid to absorb the cleaning liquid, the magnetic beads and the cleaning liquid are repeatedly blown and aspirated to mix, the nucleic acid on the magnetic beads is washed and purified, the pipette tip I completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber I into the pipette tip I, the outer magnet of the pipette tip I adsorbs the magnetic beads on the inner wall of the pipette tip I, the pipette tip I releases the cleaning liquid in the pipette tip I, and retains the magnetic beads;

[0023] S4, transfer pipette tip I to the top of cleaning liquid chamber II, the external magnet of pipette tip I leaves pipette tip I, pipette tip I moves down to the cleaning liquid to absorb the cleaning liquid, the magnetic beads and the cleaning liquid are repeatedly blown and aspirated to mix, the nucleic acid on the magnetic beads is washed and purified, pipette tip I completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber II into pipette tip I, the external magnet of pipette tip I adsorbs the magnetic beads on the inner wall of pipette tip I, pipette tip I releases the cleaning liquid in pipette tip I, and retains the magnetic beads;

[0024] S5, the pipette tip I is transferred to the top of the elution chamber, the outer magnet of the pipette tip I leaves the pipette tip I, the pipette tip I descends to the elution chamber to suck the elution, the magnetic beads and the elution are repeatedly blown and sucked to mix the nucleic acid on the magnetic beads and release them, the pipette tip I completely sucks the elution and the magnetic beads into the pipette tip I, the pipette tip I ascends to the designated position, the outer magnet adsorbs the magnetic beads on the inner wall of the pipette tip I, the pipette tip I releases the elution in the pipette tip I and drips it into the elution chamber, retaining the magnetic beads;

[0025] S6, the pipette tip I carries the magnetic beads to the top of the magnetic bead storage chamber and the magnet leaves, the pipette tip I descends to the magnetic bead storage chamber to suck up the magnetic bead diluent and mixes it by blowing and suction. The pipette tip I ascends to a specified height and releases the magnetic beads and magnetic bead diluent in the pipette tip I;

[0026] S7, the pipette tip I returns to the barrel-type pipette tip chamber I;

[0027] S8, take out the pipette tip II to the top of the elution solution, and move the pipette tip II downward to add 15 μL of the liquid into the amplification chamber of the silicon-based chip in sequence, re-dissolve the freeze-dried ball in the chamber, and then blow the re-dissolved liquid into the designated positions of the chip in sequence after re-dissolution;

[0028] S9, the pipette tip II returns to the barrel-type pipette tip chamber II;

[0029] S10, taking out the pipette tip III to absorb the UV glue in the UV glue chamber, and sealing the sample addition hole and the exhaust hole on the chip;

[0030] S11, returning the pipette tip III to the barrel tip chamber III; UV light causes the glue to quickly solidify and adhere, and the temperature rise and fall module performs the entire nucleic acid amplification process.

[0031] Compared with the prior art, the beneficial technical effects of the present invention are:

[0032] 1. The plastic injection molded parts involved in the present invention are all made by injection molding machines, which are light in weight, simple to make, can be produced with one mold and multiple cavities, and the unique supporting structure is more stable than any ordinary single tube placement. The cost price and the overall price of the single tube are about 1.5 yuan cheaper, saving consumables costs.

[0033] 2. Pre-embedded reagents can be canned in batches through a linked peristaltic pump, saving labor and mechanical site costs. If fully automatic canning is used, a reagent strip can be canned within three seconds.

[0034] 3. Advantages of silicon-based chips: Silicon-based materials do not cause immune responses, have good biocompatibility, and the consistency of experimental results is more stable than that of plastic and glass-based materials. They are suitable for detecting DNA, RNA cell pathology, protein enzymolysis, and virus and bacterial diagnosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention will be further described below in conjunction with the accompanying drawings.

[0036] Figure 1 It is a schematic diagram of the structure of the magnetic bead method nucleic acid extraction reagent strip;

[0037] Figure 2 It is a side schematic diagram of the magnetic bead method blowing and aspirating nucleic acid extraction reagent strip of the present invention;

[0038] Figure 3 This is a schematic axial view from another angle of the magnetic bead method for blowing and aspirating nucleic acid extraction reagent strip of the present invention.

[0039] Explanation of the reference numerals: 100, plate base; 101, cartridge pipette tip chamber I; 102, cartridge pipette tip chamber II; 103, cartridge pipette tip chamber III; 104, lysis and binding liquid chamber; 105, cleaning liquid chamber I; 106, cleaning liquid chamber II; 107, magnetic bead storage chamber; 108, elution liquid chamber; 109, UV glue chamber; 200, silicon-based amplification detection chip; 201, amplification chamber; 300, pipette tip I; 400, pipette tip II; 500, pipette tip III. DETAILED DESCRIPTION

[0040] The present embodiment discloses a magnetic bead method aspiration nucleic acid extraction reagent strip, which includes 7 combined chamber nucleic acid extraction strips and 1 detachable assembly silicon-based detection chip.

[0041] Specifically, a magnetic bead method for blowing and aspirating nucleic acid extraction reagent strip in this embodiment includes a plate base 100, a cylindrical pipette tip chamber I 101, a cylindrical pipette tip chamber II 102, a cylindrical pipette tip chamber III 103, a lysis and binding liquid chamber 104, a cleaning liquid chamber I 105, a cleaning liquid chamber II 106, a magnetic bead storage chamber 107, an elution liquid chamber 108, a UV glue chamber 109, and a silicon-based amplification detection chip 200 installed on the plate base 100. Specifically, the cylindrical pipette tip chamber I 101, the cylindrical pipette tip chamber II 102, the cylindrical pipette tip chamber III 103, the lysis and binding liquid chamber 104, the cleaning liquid chamber I 105, the cleaning liquid chamber II 106, the magnetic bead storage chamber 107, the elution liquid chamber 108, and the UV glue chamber 109 on the plate base 100 are integrally formed on the plate base 100 and formed by injection molding.

[0042] A pipette tip I300 is adapted and installed in the barrel tip chamber I101, a pipette tip II400 is adapted and installed in the barrel tip chamber II102, a pipette tip III500 is adapted and installed in the barrel tip chamber III103, and an amplification chamber 201 corresponding to the silicon-based amplification detection chip 200 is installed on the plate base 100.

[0043] In this embodiment, Figure 1-3 As shown, the cylindrical pipette tip chamber I 101, the cylindrical pipette tip chamber II 102, and the cylindrical pipette tip chamber III 103 are all cylindrical flat-bottom chambers.

[0044] The pipette tip I300 is a pipette tip with a volume of 1000 μL, and the pipette tip 400 and the pipette tip III500 are both pipette tips with a volume of 100 μL.

[0045] The lysis and binding liquid chamber 104 is a cylindrical round-bottom liquid chamber with a volume of 3 ml. 500 μl of lysis and binding liquid is injected into it. A heating module is installed at the bottom of the lysis and binding liquid chamber 104. Specifically, it can be an electric heating module or a water bath jacket structure.

[0046] The cleaning liquid chamber I 105 and the cleaning liquid chamber II 106 are both round-bottom chambers with a volume of 1.5 ml, and 250-300 μl of cleaning liquid is added to each of them; specifically, different cleaning liquids are added to the cleaning liquid chamber I 105 and the cleaning liquid chamber II 106, respectively.

[0047] In this embodiment, the magnetic bead storage chamber 107 is a cylindrical round-bottom chamber with a volume of 2 ml, and 80 microliters of magnetic beads are added therein; the eluent chamber 108 is a cylindrical round-bottom chamber with a volume of 2 ml, and 100 microliters of eluent are added therein; the UV glue chamber 109 is a cylindrical round-bottom chamber with a volume of 2 ml, and 300 microliters of UV glue are added therein.

[0048] like Figure 3 As shown, the amplification chamber 201 includes 4 chambers.

[0049] This embodiment provides a silicon-based chip amplification method, comprising the following steps:

[0050] S1. Collect 200 μl of sample and add it into the lysis and binding liquid chamber 104, and heat it to 60° C. through the bottom heating module.

[0051] S2. Take out the pipette tip Ⅰ300, and after repeatedly blowing and sucking the magnetic beads in the magnetic bead storage chamber 107 to mix, suck them into the pipette tip Ⅰ300. The magnet on the outside of the pipette tip Ⅰ300 is a permanent magnet. After the side of the tip is attached, it is used to adsorb the magnetic beads in the tip. The magnetic beads inside the pipette tip Ⅰ300 are adsorbed on the wall of the pipette tip Ⅰ300 tube, and the magnetic bead diluent is released and dripped into the magnetic bead storage chamber 107.

[0052] S3. The pipette tip Ⅰ300 transfers the magnetic beads to the top of the lysis and binding liquid chamber 104, the magnet outside the pipette tip Ⅰ300 leaves, the pipette tip Ⅰ300 moves downward to suck the lysis and binding liquid in the lysis and binding liquid chamber 104, and repeatedly blows and sucks to mix, and all the liquid and magnetic beads in the lysis and binding liquid chamber 104 are sucked into the pipette tip Ⅰ300, the pipette tip Ⅰ300 rises to a specified height, the external magnet of the pipette tip Ⅰ300 adsorbs the magnetic beads in the pipette tip Ⅰ300 on the inner wall of the pipette tip Ⅰ300, the pipette tip Ⅰ300 releases the lysis and binding liquid inside it and drops it into the lysis and binding liquid chamber 104, and the magnetic beads adsorbed with the sample nucleic acid are retained in the pipette tip Ⅰ300.

[0053] The pipette tip Ⅰ300 is transferred to the top of the cleaning liquid chamber Ⅰ105, the external magnet of the pipette tip Ⅰ300 leaves the pipette tip Ⅰ300, and the pipette tip Ⅰ300 moves downward into the cleaning liquid to absorb the cleaning liquid, the magnetic beads and the cleaning liquid are repeatedly blown and sucked to mix, the nucleic acid on the magnetic beads is washed and purified, the pipette tip Ⅰ300 completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber Ⅰ105 into the pipette tip Ⅰ300, the magnet outside the pipette tip Ⅰ300 adsorbs the magnetic beads on the inner wall of the pipette tip Ⅰ300, and the pipette tip Ⅰ300 releases the cleaning liquid in the pipette tip Ⅰ300 to retain the magnetic beads.

[0054] S4. The pipette tip Ⅰ300 is transferred to the top of the cleaning liquid chamber Ⅱ106. The external magnet of the pipette tip Ⅰ300 leaves the pipette tip Ⅰ300. The pipette tip Ⅰ300 moves downward into the cleaning liquid to absorb the cleaning liquid. The magnetic beads and the cleaning liquid are repeatedly blown and aspirated to mix to wash the nucleic acid on the magnetic beads and purify them. The pipette tip Ⅰ300 completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber Ⅱ106 into the pipette tip Ⅰ300. The external magnet of the pipette tip Ⅰ300 adsorbs the magnetic beads on the inner wall of the pipette tip Ⅰ300. The pipette tip Ⅰ300 releases the cleaning liquid in the pipette tip Ⅰ300 to retain the magnetic beads.

[0055] S5. The pipette tip Ⅰ300 is transferred to the top of the elution chamber 108. The external magnet of the pipette tip Ⅰ300 leaves the pipette tip Ⅰ300. The pipette tip Ⅰ300 moves downward into the elution chamber 108 to absorb the eluent. The magnetic beads and the eluent are repeatedly blown and aspirated to mix and elute the nucleic acid on the magnetic beads and release them. The pipette tip Ⅰ300 completely absorbs the eluent and the magnetic beads into the pipette tip Ⅰ300. The pipette tip Ⅰ300 moves upward to a designated position. The external magnet adsorbs the magnetic beads on the inner wall of the pipette tip Ⅰ300. The pipette tip Ⅰ300 releases the eluent in the pipette tip Ⅰ300 and drips it into the elution chamber 108 to retain the magnetic beads.

[0056] S6. The pipette tip Ⅰ300 carries the magnetic beads to the top of the magnetic bead storage chamber 107, where the magnet leaves. The pipette tip Ⅰ300 moves downward to the magnetic bead storage chamber 107, inhales the magnetic bead diluent, and mixes it by blowing and suction. The pipette tip Ⅰ300 moves upward to a specified height and releases the magnetic beads and magnetic bead diluent in the pipette tip Ⅰ300.

[0057] S7. The pipette tip I300 returns into the cylindrical tip chamber I101.

[0058] S8. Take out the pipette tip II 400 to the top of the elution liquid, and move the pipette tip II 400 downward to add 15 μl of the liquid into the amplification chamber 201 of the silicon-based chip in sequence. Redissolve the freeze-dried ball in the chamber, and then blow the reconstituted liquid into the designated positions of the chip in sequence. The detection reagent can be freeze-dried to remove moisture by using the freeze-dried ball, so that it can be stored at room temperature for a long time.

[0059] S9. The pipette tip II 400 returns into the cylindrical tip chamber II 102.

[0060] S10, take out the pipette tip III 500 to absorb the UV glue in the UV glue chamber 109, and seal the sample addition hole and the exhaust hole on the chip.

[0061] S11, returning the pipette tip III 500 to the tubular tip chamber III 103; UV light causes the glue to solidify and adhere quickly, and the temperature rise and fall module performs the entire nucleic acid amplification process.

[0062] The above embodiments are only descriptions of the preferred methods of the invention, and are not intended to limit the scope of the invention. Without departing from the design spirit of the invention, various modifications and improvements made to the technical solutions of the invention by ordinary technicians in this field should fall within the protection scope of the claims of the invention.

Claims

1. A magnetic bead method nucleic acid extraction reagent strip, characterized in that: It comprises a plate base (100), a cylindrical pipette tip chamber I (101), a cylindrical pipette tip chamber II (102), a cylindrical pipette tip chamber III (103), a lysis and binding liquid chamber (104), a cleaning liquid chamber I (105), a cleaning liquid chamber II (106), a magnetic bead storage chamber (107), an elution liquid chamber (108), a UV glue chamber (109), and a silicon-based amplification detection chip (200) arranged on the plate base (100); A pipette tip I (300) is adapted to be installed in the barrel-type pipette tip chamber I (101), a pipette tip II (400) is adapted to be installed in the barrel-type pipette tip chamber II (102), a pipette tip III (500) is adapted to be installed in the barrel-type pipette tip chamber III (103), and an amplification chamber (201) corresponding to the silicon-based amplification detection chip (200) is provided on the plate base (100).

2. The magnetic bead method nucleic acid extraction reagent strip according to claim 1, characterized in that: The cylindrical pipette tip chamber I (101), the cylindrical pipette tip chamber II (102), and the cylindrical pipette tip chamber III (103) are all cylindrical flat-bottom chambers.

3. The magnetic bead method nucleic acid extraction reagent strip according to claim 2, characterized in that: The pipette tip I (300) is a pipette tip with a volume of 1000 μL, and the pipette tip (400) and the pipette tip III (500) are both pipette tips with a volume of (100) μL.

4. The magnetic bead method nucleic acid extraction reagent strip according to claim 1, characterized in that: The lysis and binding liquid chamber (104) is a cylindrical round-bottom liquid chamber with a volume of 3 ml. (500) microliters of lysis and binding liquid are injected into it. A heating module is arranged at the bottom of the lysis and binding liquid chamber (104).

5. The magnetic bead method nucleic acid extraction reagent strip according to claim 4, characterized in that: The cleaning liquid chamber I (105) and the cleaning liquid chamber II (106) are both round-bottom chambers with a volume of 1.5 ml, and 250-300 μl of cleaning liquid is added to each of them.

6. The magnetic bead method nucleic acid extraction reagent strip according to claim 1, characterized in that: The magnetic bead storage chamber (107) is a cylindrical round-bottom chamber with a volume of 2 ml, into which 80 microliters of magnetic beads are added; the eluent chamber (108) is a cylindrical round-bottom chamber with a volume of 2 ml, into which (100) microliters of eluent are added; the UV glue chamber (109) is a cylindrical round-bottom chamber with a volume of 2 ml, into which (300) microliters of UV glue are added.

7. The magnetic bead method nucleic acid extraction reagent strip according to claim 1, characterized in that: The amplification chamber (201) includes 4 chambers.

8. The magnetic bead method nucleic acid extraction reagent strip according to claim 1, characterized in that: The cylindrical pipette tip chamber I (101), cylindrical pipette tip chamber II (102), cylindrical pipette tip chamber III (103), lysis and binding liquid chamber (104), cleaning liquid chamber I (105), cleaning liquid chamber II (106), magnetic bead storage chamber (107), elution liquid chamber (108), and UV glue chamber (109) on the plate base (100) are integrally formed on the plate base (100) and are formed by injection molding.

9. A silicon-based chip amplification method, according to any one of claims 1 to 7, wherein: The steps include: S1, collect sample and take (200) μl to add into the lysis and binding liquid chamber (104), and heat it to 70°C through the bottom heating module; S2, taking out the pipette tip I (300), and repeatedly blowing and sucking the magnetic beads in the magnetic bead storage chamber (107) to mix them, and then sucking them into the pipette tip I (300), the external magnet of the pipette tip I (300) adsorbs the magnetic beads inside the pipette tip I (300) on the tube wall of the pipette tip I (300), and releases the magnetic bead protective agent and drips it into the magnetic bead storage chamber (107); S3, the pipette tip I (300) transfers the magnetic beads to the top of the lysis and binding liquid chamber (104), the magnet outside the pipette tip I (300) leaves, the pipette tip I (300) moves downward to suck the lysis and binding liquid in the lysis and binding liquid chamber (104), and repeatedly blows and sucks to mix, so that all the liquid and magnetic beads in the lysis and binding liquid chamber (104) are sucked into the pipette tip I (300), the pipette tip I (300) rises to a specified height, the external magnet of the pipette tip I (300) adsorbs the magnetic beads in the pipette tip I (300) on the inner wall of the pipette tip I (300), the pipette tip I (300) releases the lysis and binding liquid inside it and drops it into the lysis and binding liquid chamber (104), and the magnetic beads adsorbed with the sample nucleic acid are retained in the pipette tip I (300); The pipette tip I (300) is transferred to the top of the cleaning liquid chamber I (105), the external magnet of the pipette tip I (300) leaves the pipette tip I (300), the pipette tip I (300) moves downward into the cleaning liquid to absorb the cleaning liquid, the magnetic beads and the cleaning liquid are repeatedly blown and aspirated to mix, the nucleic acid on the magnetic beads is washed and purified, the pipette tip I (300) completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber I (105) into the pipette tip I (300), the external magnet of the pipette tip I (300) adsorbs the magnetic beads on the inner wall of the pipette tip I (300), the pipette tip I (300) releases the cleaning liquid in the pipette tip I (300), and retains the magnetic beads; S4, the pipette tip I (300) is transferred to the top of the cleaning liquid chamber II (106), the external magnet of the pipette tip I (300) leaves the pipette tip I (300), the pipette tip I (300) moves downward into the cleaning liquid to absorb the cleaning liquid, the magnetic beads and the cleaning liquid are repeatedly blown and aspirated to mix, the nucleic acid on the magnetic beads is washed and purified, the pipette tip I (300) completely absorbs the cleaning liquid and magnetic beads in the cleaning liquid chamber II (106) into the pipette tip I (300), the external magnet of the pipette tip I (300) adsorbs the magnetic beads on the inner wall of the pipette tip I (300), the pipette tip I (300) releases the cleaning liquid in the pipette tip I (300), and retains the magnetic beads; S5, the pipette tip I (300) is transferred to the top of the eluent chamber (108), at which time the temperature of the heating module below the eluent (108) rises to 60°C, the external magnet of the pipette tip I (300) leaves the pipette tip I (300), the pipette tip I (300) moves downward to the eluent chamber (108) to absorb the eluent, the magnetic beads and the eluent are repeatedly blown and aspirated to mix and elute the nucleic acid on the magnetic beads and release them, the pipette tip I (300) completely absorbs the eluent and the magnetic beads into the pipette tip I (300), the pipette tip I (300) moves upward to the designated position, the external magnet adsorbs the magnetic beads on the inner wall of the pipette tip I (300), the pipette tip I (300) releases the eluent in the pipette tip I (300) and drips it into the eluent chamber (108), retaining the magnetic beads; S6, the pipette tip I (300) carries the magnetic beads to the top of the magnetic bead storage chamber (107), the magnet leaves, the pipette tip I (300) moves downward to the magnetic bead storage chamber (107), sucks in the magnetic bead protective agent, and mixes it by blowing and suction, and the pipette tip I (300) moves upward to a specified height and releases the magnetic beads and magnetic bead protective solution in the pipette tip I (300); S7, the pipette tip I (300) returns to the cylindrical pipette tip chamber I (101); S8, take out the pipette tip II (400) to the top of the elution liquid, and move the pipette tip II (400) downward to add 15 microliters of the liquid into the amplification chamber (201) of the silicon-based chip in sequence, re-dissolve the freeze-dried ball in the chamber, and then blow the re-dissolved liquid into the designated positions of the chip in sequence after re-dissolution; S9, the pipette tip II (400) returns into the cylindrical pipette tip chamber II (102); S10, taking out the pipette tip III (500) to absorb the UV glue in the UV glue chamber (109), and sealing the sample addition hole and the exhaust hole on the chip; S11, returning the pipette tip III (500) to the tubular tip chamber III (103); UV light causes the glue to solidify and adhere quickly, and the temperature rise and fall module performs the entire nucleic acid amplification process.

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