Sampling detection device for collagen matrix preparation

Abstract

The invention discloses a sampling detection device for collagen matrix preparation, and relates to the field of collagen matrix sampling, the sampling detection device comprises a base, a rotating seat, a mechanical arm and an air cylinder, the rotating seat driven by a motor is installed on the base, the mechanical arm is fixed on the rotating seat, the air cylinder is fixed on the mechanical arm, and the air cylinder is fixed on the rotating seat. A limiting mechanism is fixed to the output end of the air cylinder, a sampling mechanism is fixed to the lower end of the limiting mechanism, and a scraping mechanism is arranged on the outer side of the sampling mechanism. According to the sampling detection device for collagen matrix preparation, after sampling is completed and a sampling mechanism is separated from the liquid level of a collagen matrix, a convex shaft is driven to move through contraction of an air cylinder, and a scraping plate rotates around the outer wall of a sampling barrel in cooperation with the sliding effect between the convex shaft and a sliding groove spiral structure, so that the effect of scraping the collagen matrix on the outer wall of the sampling barrel is achieved; waste of the collagen matrix adhered to the outer wall of the sampling barrel is avoided, manual treatment is not needed in the whole operation, and the use convenience of the device is improved.

Description

Technical Field

[0001] The invention relates to the technical field of collagen matrix sampling, in particular to a sampling and detection device for collagen matrix preparation. Background Art

[0002] Collagen matrix is ​​a substance widely used in the biomedical field. It has the advantages of promoting cell growth and repair, low immunogenicity, good biodegradability and excellent hemostatic effect. After the production of collagen matrix is ​​completed, sampling and testing are required to determine the production quality of collagen matrix; At present, when sampling collagen matrix, since the collagen matrix is ​​a colloidal substance, it is necessary to manually extract the sample through a syringe during the sampling process. After sampling, a lot of collagen matrix will be adhered to the outer wall of the syringe and cannot be recovered, resulting in waste of collagen matrix. Therefore, in view of the above problem, a sampling and detection device for collagen matrix preparation is designed to better meet the actual use needs. Summary of the invention

[0003] The object of the present invention is to provide a sampling and detection device for collagen matrix preparation to solve the problems raised in the above background technology.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a sampling and detection device for preparing collagen matrix, comprising a base, a rotating base, a mechanical arm and a cylinder, wherein a rotating base driven by a motor is installed on the base, a mechanical arm is fixed on the rotating base, a cylinder is fixed on the mechanical arm, a limiting mechanism is fixed on the output end of the cylinder, a sampling mechanism is fixed on the lower end of the limiting mechanism, a scraping mechanism is arranged on the outer side of the sampling mechanism, the scraping mechanism comprises a scraper, a connecting rod, a convex shaft, a slide groove, a cylindrical rod, a limiting rod and a guide ring, the scraper contacts and slides with the outer wall of the sampling barrel, a connecting rod is fixed on the scraper, a convex shaft is fixed on the upper end of the connecting rod, the convex shaft and the slide groove are slidably connected, and the slide groove is opened on the cylindrical The rod is on the upper side of the slide groove and is a spiral structure, and the lower side of the slide groove is a strip structure. The cylindrical rod is fixed on the cylinder, and a limiting rod is also fixed on the connecting rod, and the end of the limiting rod is a spherical structure, and the spherical structure at the end of the limiting rod is nested in the groove on the guide ring for sliding. The guide ring is fixed on the fixed box, and the cylinder extends to drive the limiting mechanism and the sampling mechanism to move for sampling operation. After the sampling is completed, when the cylinder contracts to separate the sampling mechanism from the collagen matrix liquid surface, the convex shaft just cooperates with the spiral groove on the upper side of the slide groove. The cylinder continues to contract and cooperates with the sliding action between the convex shaft and the slide groove, so that the scraper can rotate. The sliding action between the scraper and the sampling barrel can realize the scraping of the collagen matrix remaining on the outer wall of the sampling barrel.

[0005] Preferably, the limiting mechanism includes a fixed box, a contact sensor, a cross bar, a first spring and a block. The fixed box is fixed at the output end of the cylinder, and a contact sensor is fixed in the fixed box, and a cross bar is slidably connected to the fixed box. Through the action of the contact sensor, a basic guarantee can be provided for the normal operation of the device.

[0006] Preferably, a first spring is fixed between the cross bar and the fixed box, and a clamping block is also fixed on the cross bar. The elastic action of the first spring can provide a basic force for the automatic reset of the clamping block, thereby ensuring the normal operation of the device.

[0007] Preferably, the sampling mechanism includes a sampling barrel, a sample storage chamber, an electric valve, a liquid inlet, a vertical rod, a piston head, a reset spring, a curved block and a push rod. The sampling barrel is fixed to the lower end of the fixed box by bolts, and sample storage chambers are opened at equal intervals in the sampling barrel, and the sample storage chamber is connected to the electric valve fixed on the sampling barrel. At the same time, the sample storage chamber and the liquid inlet are connected to each other. A sealing mechanism is fixed in the liquid inlet. The sample storage chamber and the liquid inlet can ensure normal sampling. In combination with the electric valve, the air pressure inside the sample storage chamber can be balanced to ensure the normal operation of the device.

[0008] Preferably, the sampling barrel is slidably connected to a vertical rod, and piston heads are fixed on the vertical rod at equal intervals, and the piston heads and the sample storage chambers are distributed in a one-to-one correspondence. At the same time, the piston head and the sample storage chamber are slidably connected, and a reset spring is also fixed between the piston head and the sample storage chamber. Through the sliding action between the piston head and the sample storage chamber, the sample can be sucked, ensuring the normal sampling, and through the elastic action of the reset spring, it can provide a basic guarantee for the automatic removal of samples in the later stage.

[0009] Preferably, an arc block is fixed to the upper end of the vertical rod, and the arc block and the clamping block are snap-fitted, and a push rod is also fixed to the upper end of the arc block. Through the snap-fitting action between the arc block and the clamping block, the positioning of the vertical rod and the piston head can be achieved to ensure the normal operation of the device.

[0010] Preferably, the push rod is located directly below the contact sensor, and the height of the push rod and the arc block is equal to the distance between the card block and the contact sensor. Through the action of the push rod and the contact sensor, a basic guarantee can be provided for the automatic control of the device.

[0011] Preferably, the sealing mechanism includes a partition, a sealing plug, a fixed rod, a fixed plate, a sliding rod and a second spring. The partition is fixed in the liquid inlet, and the partition and the sealing plug are nested. The sealing effect between the partition and the sealing plug can effectively prevent sample overflow.

[0012] Preferably, the sealing plug is fixed to one end of the fixed rod, and the other end of the fixed rod is fixed to the fixed plate, and a sliding rod is fixed to the fixed plate, and the sliding rod and the partition are slidably connected. When the sealing plug and the fixed plate move, the sliding guiding effect between the sliding rod and the partition can ensure the stability of the movement of the sealing plug and the fixed plate.

[0013] Preferably, the fixing plate and one end of the second spring are fixed to each other, and the other end of the second spring is fixed to the partition plate, and the elastic action of the second spring can provide a basic force for the automatic reset of the fixing plate and the sealing plug.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The sampling and detection device for preparation of collagen matrix, after sampling is completed, when the sampling mechanism is separated from the collagen matrix liquid surface, the cylinder contracts to drive the convex shaft to move, and the sliding action between the convex shaft and the spiral structure of the slide groove makes the scraper rotate around the outer wall of the sampling barrel, so as to scrape away the collagen matrix on the outer wall of the sampling barrel, thereby avoiding the waste of the collagen matrix adhered to the outer wall of the sampling barrel, and the whole operation does not require manual processing, thereby improving the convenience of using the device; 2. The sampling and detection device for collagen matrix preparation can enable the limiting mechanism and the sampling mechanism to enter the storage container through the cylinder during sampling. With the cooperation of the vertical rod, the piston head, the electric valve, and the liquid inlet, the sample extraction at multiple positions can be realized, thereby realizing the sampling function. After the sampling is completed, the vertical rod can be locked through the action of the cross bar, the first spring, the clamping block, and the arc surface block to avoid sample exclusion and ensure the normal sampling. The entire sampling process is controlled by the operation of the mechanical arm and the cylinder, without the need for manual intervention, thereby reducing the labor intensity of workers. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the device of the present invention from a front view; Figure 2 It is a front view structural diagram of the cylinder, the limiting mechanism and the sampling mechanism of the present invention; Figure 3 This is a schematic diagram of a three-dimensional structure of a practical limit mechanism and a scraper mechanism in a front view; Figure 4 It is a schematic diagram of the front cross-sectional three-dimensional structure of the limiting mechanism of the present invention; Figure 5 For the present invention Figure 4 The enlarged structural diagram at A in the middle; Figure 6 It is a schematic diagram of the front cross-sectional three-dimensional structure of the sampling mechanism of the present invention; Figure 7 It is a schematic diagram of the three-dimensional structure of the sealing mechanism of the present invention.

[0016] In the figure: 1, base; 2, rotating seat; 3, mechanical arm; 4, cylinder; 5, limiting mechanism; 501, fixed box; 502, contact sensor; 503, cross bar; 504, first spring; 505, block; 6, sampling mechanism; 601, sampling barrel; 602, sample storage chamber; 603, electric valve; 604, liquid inlet; 605, vertical rod; 606, piston head; 6061, reset spring; 607, arc block; 608, push rod; 7, sealing mechanism; 701, partition; 702, sealing plug; 703, fixed rod; 704, fixed plate; 705, slide rod; 706, second spring; 8, scraping mechanism; 801, scraper; 802, connecting rod; 803, convex shaft; 804, slide groove; 805, cylindrical rod; 806, limiting rod; 807, guide ring. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] See also Figure 1-Figure 7The present invention provides a technical solution: a sampling and detection device for preparing collagen matrix, comprising a base 1, a rotating base 2, a mechanical arm 3 and a cylinder 4, wherein the base 1 is provided with a rotating base 2 driven by a motor, the rotating base 2 is fixed with a mechanical arm 3, the mechanical arm 3 is fixed with a cylinder 4, the output end of the cylinder 4 is fixed with a limiting mechanism 5, the lower end of the limiting mechanism 5 is fixed with a sampling mechanism 6, a scraping mechanism 8 is arranged on the outer side of the sampling mechanism 6, the scraping mechanism 8 comprises a scraper 801, a connecting rod 802, a convex shaft 803, a slide groove 804, a cylindrical rod 805, a limiting rod 806 and a guide ring 807, the scraper 801 contacts and slides with the outer wall of the sampling barrel 601, the scraper 801 is fixed with a connecting rod 802, the upper end of the connecting rod 802 is fixed with a convex shaft 803, the convex shaft 803 and the slide groove 804 are slidably connected, the slide groove 804 is arranged on the cylindrical rod 805, and the slide groove 804 The upper side is a spiral structure, and the lower side of the slide groove 804 is a strip structure. The cylindrical rod 805 is fixed on the cylinder 4. The limiting rod 806 is also fixed on the connecting rod 802, and the end of the limiting rod 806 is a spherical structure, and the spherical structure at the end of the limiting rod 806 is nested in the groove on the guide ring 807 for sliding. The guide ring 807 is fixed on the fixed box 501. The cylinder 4 extends to drive the limiting mechanism 5 and the sampling mechanism 6 to move for sampling operation. After the sampling is completed, when the cylinder 4 contracts to separate the sampling mechanism 6 from the collagen matrix liquid surface, the convex shaft 803 just cooperates with the spiral groove on the upper side of the slide groove 804. The cylinder 4 continues to contract and cooperates with the sliding action between the convex shaft 803 and the slide groove 804, so that the scraper 801 can rotate. The sliding action between the scraper 801 and the sampling barrel 601 is used to scrape off the collagen matrix remaining on the outer wall of the sampling barrel 601.

[0019] When using the sampling and detection device for preparing collagen matrix, Figure 1-Figure 7 As shown, when sampling is performed, the positions of the cylinder 4 and the sampling mechanism 6 can be adjusted through the action of the rotating seat 2 and the mechanical arm 3 to ensure that the distance from the lower end surface of the sampling mechanism 6 to the collagen matrix liquid surface is greater than the height of the threaded groove on the slide 804. After the position adjustment of the sampling mechanism 6 is completed, the cylinder 4 is controlled to extend, thereby driving the limit mechanism 5, the sampling mechanism 6 and the convex shaft 803 to move downward. When the convex shaft 803 moves from the threaded groove on the slide 804 to the strip groove on the slide 804, the sampling mechanism 6 does not contact the collagen matrix liquid surface at this time, thereby preventing the convex shaft 803 from sliding in the threaded groove on the slide 804 to drive the scraper 801 to rotate and stirring the collagen matrix, thereby ensuring the normal progress of subsequent sampling; The sampling mechanism 6 includes a sampling barrel 601, a sample storage chamber 602, an electric valve 603, a liquid inlet 604, a vertical rod 605, a piston head 606, a return spring 6061, a curved surface block 607 and a push rod 608. The sampling barrel 601 is fixed to the lower end of the fixed box 501 by bolts, and the sampling barrel 601 is provided with sample storage chambers 602 at equal intervals, and the sample storage chambers 602 are connected to the electric valve 603 fixed on the sampling barrel 601. At the same time, the sample storage chamber 602 is connected to the liquid inlet. The ports 604 are connected to each other, and a sealing mechanism 7 is fixed in the liquid inlet 604; a vertical rod 605 is slidably connected to the sampling barrel 601, and piston heads 606 are fixed at equal intervals on the vertical rod 605, and the piston heads 606 and the sample storage chamber 602 are distributed one by one, and the piston heads 606 and the sample storage chamber 602 are slidably connected, and a return spring 6061 is also fixed between the piston heads 606 and the sample storage chamber 602; an arc block 607 is fixed on the upper end of the vertical rod 605 , and the arc block 607 is connected with the clamping block 505 by a snap-fit ​​connection, and a push rod 608 is fixed on the upper end of the arc block 607; the push rod 608 is located directly below the contact sensor 502, and the height of the push rod 608 and the arc block 607 is equal to the distance between the clamping block 505 and the contact sensor 502; the sealing mechanism 7 includes a partition 701, a sealing plug 702, a fixing rod 703, a fixing plate 704, a sliding rod 705 and a second spring 706, and the partition 701 is fixed In the liquid inlet 604, the partition 701 and the sealing plug 702 are nested and connected; the sealing plug 702 is fixed to one end of the fixing rod 703, and the other end of the fixing rod 703 is fixed to the fixing plate 704, and a sliding rod 705 is fixed to the fixing plate 704, and the sliding rod 705 and the partition 701 are slidably connected; the fixing plate 704 and one end of the second spring 706 are fixed to each other, and the other end of the second spring 706 is fixed to the partition 701; When the cylinder 4 continues to extend and drives the limit mechanism 5, the sampling mechanism 6 and the convex shaft 803 to move downward, Figure 1-Figure 7As shown, at this time, the scraper 801 does not rotate. During the downward movement of the sampling mechanism 6, when the vertical rod 605 contacts the bottom surface of the collagen matrix storage container, the electric valve 603 is opened to balance the air pressure at the upper end of the sample storage chamber 602, and the vertical rod 605 is forced to slide upward relative to the sampling barrel 601, thereby driving the piston head 606 to slide upward in the sample storage chamber 602, so that a negative pressure is formed in the space below the sample storage chamber 602 and the piston head 606. Under the action of the air pressure, the sealing plug 702 in the liquid inlet 604 is forced to move, and the sliding rod 70 The sliding guide effect between the sealing plug 702 and the partition 701 can ensure the stability of the movement of the sealing plug 702. When the sealing plug 702 is separated from the opening on the partition 701, the sealing effect is released. At this time, under the negative pressure in the sample storage cavity 602 and the lower space of the piston head 606, the collagen matrix enters the sample storage cavity 602 through the liquid inlet 604 for storage, thereby realizing the sampling effect. Through the effect of multiple sample storage cavities 602, the collagen matrix at different positions in the collagen matrix storage container can be sampled, so as to facilitate the accuracy of subsequent detection data. The limiting mechanism 5 includes a fixed box 501, a contact sensor 502, a cross bar 503, a first spring 504 and a clamping block 505. The fixed box 501 is fixed to the output end of the cylinder 4, and the contact sensor 502 is fixed in the fixed box 501, and the cross bar 503 is slidably connected to the fixed box 501; the first spring 504 is fixed between the cross bar 503 and the fixed box 501, and the clamping block 505 is also fixed on the cross bar 503; When the vertical rod 605 is forced to slide upward relative to the sampling barrel 601, Figure 1-Figure 7 As shown, the vertical rod 605 moves synchronously to drive the arc block 607 and the push rod 608 to move. When the arc block 607 contacts and slides with the inclined surface of the block 505, the block 505 is forced to move. With the sliding guide effect between the cross bar 503 and the fixed box 501, the stability of the movement of the block 505 can be ensured. When the push rod 608 contacts the contact sensor 502, the contact sensor 502 transmits a signal to the controller, so that the cylinder 4 stops extending, and the electric valve 603 is closed at this time, thereby completing the sampling operation. After the pressure in the space under the piston head 606 is balanced, under the action of the second spring 706, the sealing plug 702 is reset to seal the opening on the partition 701 to prevent the sample from overflowing. At this time, the arc block 607 moves to the top of the block 505, and with the elastic effect of the first spring 504, the block 505 is reset and engaged with the lower end surface of the arc block 607, thereby realizing the positioning effect of the vertical rod 605. After sampling is completed, Figure 1-Figure 7As shown, the cylinder 4 is controlled to contract to drive the limiting mechanism 5, the sampling mechanism 6 and the convex shaft 803 to move upward. When the lower end surface of the sampling barrel 601 is separated from the collagen matrix liquid surface, the convex shaft 803 slides in the strip groove on the slide groove 804. When the cylinder 4 continues to contract and drives the convex shaft 803 to move upward to slide in the spiral groove on the slide groove 804, the scraper 801 and the connecting rod 802 can be driven to rotate. The sliding effect of the spherical structure at the end of the limiting rod 806 nesting between the slots of the guide ring 807 can ensure the rotation stability of the scraper 801 and the connecting rod 802. Through the rotation of the scraper 801, the collagen matrix bonded to the outer surface of the sampling barrel 601 can be scraped off, and the scraped collagen matrix falls to the collagen matrix storage tank. The sample is stored in the container to avoid wasting the collagen matrix until the cylinder 4 and the convex shaft 803 return to their initial positions, thereby completing the sampling operation. When removing the sample later, it is only necessary to open the electric valve 603 and then pull the cross bar 503 to separate the block 505 from the arc block 607, thereby releasing the limiting effect, and with the action of the reset spring 6061, the piston head 606 slides downward in the sample storage chamber 602, thereby squeezing out the sample stored in the sample storage chamber 602 for subsequent detection. When the sample is extruded, under the action of the extrusion pressure, the sealing plug 702 can be moved and separated from the partition 701 to release the sealing effect, thereby facilitating the normal discharge of the sample. This is the working principle of the sampling and detection device for collagen matrix preparation.

[0020] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sampling and detection device for preparing a collagen matrix, comprising a base (1), a rotating base (2), a mechanical arm (3) and a cylinder (4), wherein the base (1) is mounted with a rotating base (2) driven by a motor, the rotating base (2) is fixed with a mechanical arm (3), and the mechanical arm (3) is fixed with a cylinder (4), characterized in that: A limiting mechanism (5) is fixed at the output end of the cylinder (4), a sampling mechanism (6) is fixed at the lower end of the limiting mechanism (5), a scraping mechanism (8) is arranged outside the sampling mechanism (6), the scraping mechanism (8) comprises a scraper (801), a connecting rod (802), a convex shaft (803), a slide groove (804), a cylindrical rod (805), a limiting rod (806) and a guide ring (807), the scraper (801) contacts and slides with the outer wall of the sampling barrel (601), a connecting rod (802) is fixed on the scraper (801), a convex shaft (803) is fixed on the upper end of the connecting rod (802), the convex shaft (803) and the slide groove (804) are connected to each other. 04) are connected by sliding connection, the slide groove (804) is opened on the cylindrical rod (805), and the upper side of the slide groove (804) is a spiral structure, and the lower side of the slide groove (804) is a strip structure, the cylindrical rod (805) is fixed on the cylinder (4), and a limit rod (806) is also fixed on the connecting rod (802), and the end of the limit rod (806) is a spherical structure, and the spherical structure at the end of the limit rod (806) is nested in the groove on the guide ring (807) for sliding, and the guide ring (807) is fixed on the fixed box (501), and the cylinder (4) extends to drive the limit mechanism (5) and the sampling mechanism (6) to move to perform the sampling operation.

2. A sampling and detection device for preparing collagen matrix according to claim 1, characterized in that: The limiting mechanism (5) comprises a fixed box (501), a contact sensor (502), a cross bar (503), a first spring (504) and a clamping block (505); the fixed box (501) is fixed to the output end of the cylinder (4), the contact sensor (502) is fixed in the fixed box (501), and the cross bar (503) is slidably connected to the fixed box (501).

3. A sampling and detection device for preparing collagen matrix according to claim 2, characterized in that: A first spring (504) is fixed between the cross bar (503) and the fixed box (501), and a clamping block (505) is also fixed on the cross bar (503).

4. A sampling and detection device for preparing collagen matrix according to claim 3, characterized in that: The sampling mechanism (6) comprises a sampling barrel (601), a sample storage chamber (602), an electric valve (603), a liquid inlet (604), a vertical rod (605), a piston head (606), a return spring (6061), a curved surface block (607) and a push rod (608); the sampling barrel (601) is fixed to the lower end of the fixed box (501) by bolts, and the sampling barrel (601) is provided with sample storage chambers (602) at equal intervals, and the sample storage chambers (602) are connected to the electric valve (603) fixed on the sampling barrel (601); the sample storage chamber (602) and the liquid inlet (604) are connected to each other, and a sealing mechanism (7) is fixed in the liquid inlet (604).

5. A sampling and detection device for preparing collagen matrix according to claim 4, characterized in that: The sampling barrel (601) is slidably connected to a vertical rod (605), and piston heads (606) are fixed on the vertical rod (605) at equal intervals, and the piston heads (606) and the sample storage chamber (602) are distributed in a one-to-one correspondence. At the same time, the piston head (606) and the sample storage chamber (602) are slidably connected, and a return spring (6061) is also fixed between the piston head (606) and the sample storage chamber (602).

6. A sampling and detection device for preparing collagen matrix according to claim 4, characterized in that: A curved surface block (607) is fixed to the upper end of the vertical rod (605), and the curved surface block (607) is in a snap-fit ​​connection with the clamping block (505), and a top rod (608) is also fixed to the upper end of the curved surface block (607).

7. A sampling and detection device for preparing collagen matrix according to claim 4, characterized in that: The push rod (608) is located directly below the contact sensor (502), and the height of the push rod (608) and the arc surface block (607) is equal to the distance between the clamping block (505) and the contact sensor (502).

8. A sampling and detection device for preparing collagen matrix according to claim 4, characterized in that: The sealing mechanism (7) comprises a partition (701), a sealing plug (702), a fixing rod (703), a fixing plate (704), a sliding rod (705) and a second spring (706); the partition (701) is fixed in the liquid inlet (604), and the partition (701) and the sealing plug (702) are nestedly connected.

9. A sampling and detection device for preparing collagen matrix according to claim 8, characterized in that: The sealing plug (702) is fixed to one end of the fixing rod (703), and the other end of the fixing rod (703) is fixed to the fixing plate (704). A sliding rod (705) is fixed to the fixing plate (704), and the sliding rod (705) is slidably connected to the partition plate (701).

10. A sampling and detection device for preparing collagen matrix according to claim 8, characterized in that: The fixing plate (704) and one end of the second spring (706) are fixed to each other, and the other end of the second spring (706) is fixed to the partition plate (701).

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