Papaya-containing composition capable of improving bioavailability of glucoraphanin and preparation method thereof

Abstract

The invention discloses a papaya-containing composition capable of improving the bioavailability of glucoraphanin and a preparation method of the papaya-containing composition. The composition comprises a broccoli seed aqueous extract, a papaya extract and a vitamin C solution, and the broccoli seed aqueous extract, the papaya extract and the vitamin C solution are mixed according to the mass ratio of (10-30): (1-5): 1; according to the composition, the utilization rate of glucoraphanin is increased, and glucoraphanin can be stably and efficiently converted into sulforaphane; the conversion rate of glucoraphanin can be up to 45%, and the preparation process, method and the like are safe and stable and are convenient to operate and produce.

Description

Technical Field

[0001] The invention relates to the field of food production, and in particular to a composition containing papaya and capable of improving the bioavailability of glucoraphanin and a preparation method thereof. Background Art

[0002] Broccoli is known as the "crown of vegetables". It is rich in basic nutrients and various active ingredients needed by the human body, such as glucosinolates, polyphenols, vitamin C, etc. Glucoraphanin is 4-methylsulfinylbutyl glucosinolate, and the R group is CH3SO(CH2)4. It belongs to aliphatic glucosinolates, and its degradation products are isothiocyanates, mainly sulforaphane. Sulforaphane is the most powerful isothiocyanate found in vegetables so far. As a natural product with strong biological activity, sulforaphane has shown excellent pharmacological effects in the fields of anti-cancer, anti-inflammatory and antioxidant, but its stability is very poor and is greatly affected by factors such as temperature and pH. It degrades particularly fast in water and under high temperature conditions, and its application is greatly limited. Therefore, there are very few sulforaphane health products or pharmaceutical products on the market. Although single glucoraphane has good stability, its biological absorption and utilization rate in the human body is very low. It needs to be partially converted into sulforaphane after the action of intestinal microorganisms, and the conversion rate is only 5%-10%. To this end, the present invention provides a composition containing papaya for improving the bioavailability of glucoraphanin and a preparation method thereof, so as to solve the defects and shortcomings of the prior art. Summary of the invention

[0003] In view of the above problems, the present invention provides a composition containing papaya for improving the bioavailability of glucoraphanin and a preparation method thereof.

[0004] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions:

[0005] A papaya-containing composition for improving the bioavailability of glucoraphanin, characterized in that it comprises broccoli seed water extract, papaya extract and vitamin C solution;

[0006] The broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of (10-30):(1-5):1;

[0007] Wherein, the concentration of the vitamin C solution is 1.05-1.25mmol / L.

[0008] Preferably, the broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of (15-25):(2-4):1 in mass percentage.

[0009] Preferably, the broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of 20:3:1 in mass percentage.

[0010] Preferably, the broccoli seed water extract contains 15%-18.5% glucoraphanin.

[0011] Preferably, the concentration of the vitamin C solution is 1.15 mmol / L.

[0012] Preferably, the preparation method of the composition containing papaya for improving the bioavailability of glucoraphanin comprises the following steps:

[0013] S1: preparing the papaya extract;

[0014] (1) Papaya seeds are crushed and sieved with liquid nitrogen, 0.04 mol / L of L-cys reagent and 0.002 mol / L of EDTA are added, 2-3 times the mass of the papaya and 60%-70% ethanol is added after mixing, the temperature is raised to 60-65° C., the pH of the mixed solution is adjusted to 5.8-6.2, and extraction is performed by immersion method;

[0015] (2) taking the supernatant and concentrating it to 20%-30% of the original volume, adding it to a solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying them to obtain the papaya extract;

[0016] S2: preparation of broccoli seed aqueous extract;

[0017] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 100-200 mesh sieve to obtain broccoli seed pulverized material;

[0018] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0019] (3) The supernatant is then concentrated to 20%-30% of the original volume, followed by alcohol precipitation, and finally filtered to obtain a filtrate;

[0020] (4) further concentrating the filtrate to 20%-30% of the original volume, and finally obtaining the broccoli seed water extract by spray drying;

[0021] S3: mixing the prepared papaya extract, broccoli seed water extract and vitamin C solution to obtain the composition;

[0022] S4: Add 0.9% NaCl solution to the composition in a vacuum mixer, adjust the pH of the solution to 5-7, adjust the reaction temperature to 40-50° C., add myrosinase, mix well and react for 30-45 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0023] Preferably, the extraction method of the myrosinase is:

[0024] (1) Grind broccoli seeds with liquid nitrogen to crush cells, then add petroleum ether, apply ultrasonic treatment for 5-10 minutes, and then stand for 10-12 hours to defat;

[0025] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 18-22° C., removing petroleum ether, and obtaining the myrosinase.

[0026] Due to the adoption of the above technical scheme, the beneficial effects of the present invention are as follows: the composition of the present invention improves the utilization rate of glucoraphanin and can be stably and efficiently converted into sulforaphane; the addition of the papaya extract and vitamin C of the present invention can directly affect the conversion rate of glucoraphanin. When the papaya extract and vitamin C are added at a specific mass ratio, the conversion rate of glucoraphanin can be as high as 45%. The preparation process and method of the present invention are safe and stable and easy to operate and produce. DETAILED DESCRIPTION

[0027] To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Embodiment 1:

[0029] This embodiment 1 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation of the composition specifically includes the following steps:

[0030] S1: preparing papaya extract;

[0031] (1) Papaya seeds were crushed and sieved with liquid nitrogen, 0.04 mol / L L-cys reagent and 0.002 mol / L EDTA were added, and after mixing, 65% ethanol with a concentration of 2-3 times the mass of the papaya was added, the temperature was raised to 62° C., the pH of the mixed solution was adjusted to 6.0, and the mixture was extracted by immersion method;

[0032] (2) taking the supernatant and concentrating it to 25% of the original volume, adding it to a 55% methanol solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying to obtain a papaya extract;

[0033] S2: preparation of broccoli seed aqueous extract;

[0034] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 150-mesh sieve to obtain broccoli seed pulverized material;

[0035] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0036] (3) The supernatant is then concentrated to 25% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0037] (4) further concentrating the filtrate to 25% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0038] The inlet temperature was controlled at 140°C, the outlet temperature was controlled at 98°C, the centrifugal nozzle speed was controlled at 2600rpm, the feed rate was controlled at 55mL / h, and the drying time was 5s;

[0039] S3: Mixing the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:3:1 to obtain a composition;

[0040] S4: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.0, adjust the reaction temperature to 45° C., add the extracted myrosinase, mix well and react for 35 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0041] Wherein, the extraction method of myrosinase is:

[0042] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0043] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase, which is a crude myrosinase with a low purity.

[0044] Embodiment 2:

[0045] This Example 2 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation of the composition specifically includes the following steps:

[0046] S1: preparing papaya extract;

[0047] (1) Papaya seeds were crushed and sieved with liquid nitrogen, 0.04 mol / L L-cys reagent and 0.002 mol / L EDTA were added, and after mixing, 70% ethanol with a concentration of 2-3 times the mass of the papaya was added, the temperature was raised to 65° C., the pH of the mixed solution was adjusted to 6.2, and the mixture was extracted by immersion method;

[0048] (2) taking the supernatant and concentrating it to 30% of the original volume, adding it to a 55% methanol solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying to obtain a papaya extract;

[0049] S2: preparation of broccoli seed aqueous extract;

[0050] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 120-mesh sieve to obtain broccoli seed pulverized material;

[0051] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0052] (3) The supernatant is then concentrated to 30% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0053] (4) further concentrating the filtrate to 30% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0054] Control the inlet temperature at 135-145℃, the outlet temperature at 95-100℃, the centrifugal nozzle speed at 2500-3000rpm, the feed rate at 35-70mL / h, and the drying time at 4.5-6s;

[0055] S3: Mixing the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.25 mmol / L in a mass ratio of 15:2:1 to obtain a composition;

[0056] S4: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.1, adjust the reaction temperature to 42° C., add the extracted myrosinase, mix well and react for 40 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0057] Wherein, the extraction method of myrosinase is:

[0058] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0059] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0060] Embodiment 3:

[0061] This Example 3 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation of the composition specifically includes the following steps:

[0062] S1: preparing papaya extract;

[0063] (1) Papaya seeds were crushed and sieved with liquid nitrogen, 0.04 mol / L L-cys reagent and 0.002 mol / L EDTA were added, and after mixing, 60% ethanol with a concentration of 2-3 times the mass of the papaya was added, the temperature was raised to 60° C., the pH of the mixed solution was adjusted to 5.8, and the mixture was extracted by immersion method;

[0064] (2) taking the supernatant and concentrating it to 20% of the original volume, adding it to a 55% methanol solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying to obtain a papaya extract;

[0065] S2: preparation of broccoli seed aqueous extract;

[0066] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 170-mesh sieve to obtain broccoli seed pulverized material;

[0067] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0068] (3) The supernatant is then concentrated to 20% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0069] (4) further concentrating the filtrate to 20% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0070] Control the inlet temperature at 135-145℃, the outlet temperature at 95-100℃, the centrifugal nozzle speed at 2500-3000rpm, the feed rate at 35-70mL / h, and the drying time at 4.5-6s;

[0071] S3: Mixing the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.05 mmol / L in a mass ratio of 25:4:1 to obtain a composition;

[0072] S4: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 5.8, adjust the reaction temperature to 48°C, add the extracted myrosinase, mix well and react for 35 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0073] Wherein, the extraction method of myrosinase is:

[0074] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0075] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0076] Embodiment 4:

[0077] This Example 4 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation of the composition specifically includes the following steps:

[0078] S1: preparing papaya extract;

[0079] (1) Papaya seeds were crushed and sieved with liquid nitrogen, 0.04 mol / L L-cys reagent and 0.002 mol / L EDTA were added, and after mixing, 62% ethanol with a concentration of 2-3 times the mass of the papaya was added, the temperature was raised to 64° C., the pH of the mixed solution was adjusted to 5.9, and the mixture was extracted by immersion method;

[0080] (2) taking the supernatant and concentrating it to 26% of the original volume, adding it to a 55% methanol solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying to obtain a papaya extract;

[0081] S2: preparation of broccoli seed aqueous extract;

[0082] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 200-mesh sieve to obtain broccoli seed pulverized material;

[0083] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0084] (3) The supernatant is then concentrated to 25% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0085] (4) further concentrating the filtrate to 25% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0086] Control the inlet temperature at 135-145℃, the outlet temperature at 95-100℃, the centrifugal nozzle speed at 2500-3000rpm, the feed rate at 35-70mL / h, and the drying time at 4.5-6s;

[0087] S3: Mixing the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.2 mmol / L in a mass ratio of 30:5:1 to obtain a composition;

[0088] S4: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.4, adjust the reaction temperature to 45° C., add the extracted myrosinase, mix well and react for 40 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0089] Wherein, the extraction method of myrosinase is:

[0090] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0091] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0092] Embodiment 5:

[0093] This Example 5 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation of the composition specifically includes the following steps:

[0094] S1: preparing papaya extract;

[0095] (1) Papaya seeds were crushed and sieved with liquid nitrogen, 0.04 mol / L L-cys reagent and 0.002 mol / L EDTA were added, and after mixing, 63% ethanol with a concentration of 2-3 times the mass of the papaya was added, the temperature was raised to 62° C., the pH of the mixed solution was adjusted to 6.0, and the mixture was extracted by immersion method;

[0096] (2) taking the supernatant and concentrating it to 30% of the original volume, adding it to a 55% methanol solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying to obtain a papaya extract;

[0097] S2: preparation of broccoli seed aqueous extract;

[0098] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 100-mesh sieve to obtain broccoli seed pulverized material;

[0099] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0100] (3) The supernatant is then concentrated to 20% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0101] (4) further concentrating the filtrate to 30% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0102] Control the inlet temperature at 135-145℃, the outlet temperature at 95-100℃, the centrifugal nozzle speed at 2500-3000rpm, the feed rate at 35-70mL / h, and the drying time at 4.5-6s;

[0103] S3: Mixing the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.1 mmol / L in a mass ratio of 10:1:1 to obtain a composition;

[0104] S4: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.7, adjust the reaction temperature to 45°C, add the extracted myrosinase, mix well and react for 30 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0105] Wherein, the extraction method of myrosinase is:

[0106] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0107] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0108] Embodiment 6:

[0109] The preparation conditions and methods of Example 1 in this Example 6 are the same, and the only difference is in the S3 step group, which is specifically:

[0110] The papaya extract, the broccoli seed water extract containing 16% glucoraphanin and the vitamin C solution with a concentration of 1.05 mmol / L were mixed in a mass ratio of 20:3:1 to obtain a composition.

[0111] Embodiment 7:

[0112] The preparation conditions and methods of Example 1 in this Example 7 are the same, and the only difference is in the S3 step group, which is specifically:

[0113] The papaya extract, the broccoli seed water extract containing 16% glucoraphanin and the vitamin C solution with a concentration of 1.25 mmol / L are mixed in a mass ratio of 20:3:1 to obtain a composition.

[0114] Comparative Example 1:

[0115] The preparation conditions and methods of this comparative example 1 and embodiment 1 are the same, and the only difference is in the step group S3, which is specifically:

[0116] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 0.1:3:1 to obtain a composition.

[0117] Comparative Example 2:

[0118] The preparation conditions and methods of Comparative Example 2 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0119] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 1:3:1 to obtain a composition.

[0120] Comparative Example 3:

[0121] The preparation conditions and methods of this comparative example 3 and example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0122] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 5:3:1 to obtain a composition.

[0123] Comparative Example 4:

[0124] The preparation conditions and methods of Comparative Example 4 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0125] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 40:3:1 to obtain a composition.

[0126] Comparative Example 5:

[0127] The preparation conditions and methods of Comparative Example 5 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0128] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 50:3:1 to obtain a composition.

[0129] Comparative Example 6:

[0130] Comparative Example 6 is a method for preparing a composition capable of improving the bioavailability of glucoraphanin. The preparation of the composition specifically comprises the following steps:

[0131] S1: preparation of broccoli seed aqueous extract;

[0132] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 150-mesh sieve to obtain broccoli seed pulverized material;

[0133] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0134] (3) The supernatant is then concentrated to 25% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0135] (4) further concentrating the filtrate to 25% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0136] The inlet temperature was controlled at 140°C, the outlet temperature was controlled at 98°C, the centrifugal nozzle speed was controlled at 2600rpm, the feed rate was controlled at 55mL / h, and the drying time was 5s;

[0137] S2: mixing a broccoli seed water extract containing 16% glucoraphanin and a vitamin C solution having a concentration of 1.15 mmol / L at a mass ratio of 20:1 to obtain a composition;

[0138] S3: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.0, adjust the reaction temperature to 45° C., add the extracted myrosinase, mix well and react for 35 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0139] Wherein, the extraction method of myrosinase is:

[0140] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0141] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0142] Comparative Example 7:

[0143] The preparation conditions and methods of Comparative Example 7 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0144] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:0.1:1 to obtain a composition.

[0145] Comparative Example 8:

[0146] The preparation conditions and methods of Comparative Example 8 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0147] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:7:1 to obtain a composition.

[0148] Comparative Example 9:

[0149] The preparation conditions and methods of Comparative Example 9 and Example 1 are the same, and the only difference is in the S3 step group, which is specifically:

[0150] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:10:1 to obtain a composition.

[0151] Comparative Example 10:

[0152] Comparative Example 10 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0153] S3: Mix the papaya extract and the broccoli seed water extract containing 16% glucoraphanin in a mass ratio of 20:3 to obtain a composition.

[0154] Comparative Example 11:

[0155] Comparative Example 11 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0156] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:3:0.1 to obtain a composition.

[0157] Comparative Example 12:

[0158] Comparative Example 12 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0159] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:3:3 to obtain a composition.

[0160] Comparative Example 13:

[0161] Comparative Example 13 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0162] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.15 mmol / L in a mass ratio of 20:3:5 to obtain a composition.

[0163] Comparative Example 14:

[0164] Comparative Example 14 is a method for preparing a composition containing papaya for improving the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0165] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 0.5 mmol / L in a mass ratio of 20:3:1 to obtain a composition.

[0166] Comparative Example 15:

[0167] Comparative Example 15 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0168] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 0.8 mmol / L in a mass ratio of 20:3:1 to obtain a composition.

[0169] Comparative Example 16:

[0170] Comparative Example 16 is a method for preparing a composition containing papaya that can improve the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0171] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.35 mmol / L in a mass ratio of 20:3:1 to obtain a composition.

[0172] Comparative Example 17:

[0173] Comparative Example 17 is a method for preparing a composition containing papaya for improving the bioavailability of glucoraphanin. The preparation steps of the composition are different from those of Example 1 only in step S3, which are specifically as follows:

[0174] S3: Mix the papaya extract, the broccoli seed water extract containing 16% glucoraphanin, and the vitamin C solution with a concentration of 1.45 mmol / L in a mass ratio of 20:3:1 to obtain a composition.

[0175] Comparative Example 18:

[0176] The preparation method of the composition of this comparative example 18 specifically comprises the following steps:

[0177] S1: preparation of broccoli seed aqueous extract;

[0178] (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 150-mesh sieve to obtain broccoli seed pulverized material;

[0179] (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation;

[0180] (3) The supernatant is then concentrated to 25% of the original volume, precipitated with alcohol, and finally filtered to obtain a filtrate;

[0181] (4) further concentrating the filtrate to 25% of the original volume, and finally spray drying to obtain a broccoli seed aqueous extract;

[0182] The inlet temperature was controlled at 140°C, the outlet temperature was controlled at 98°C, the centrifugal nozzle speed was controlled at 2600rpm, the feed rate was controlled at 55mL / h, and the drying time was controlled at 5s to obtain a composite;

[0183] S2: Add 0.9% NaCl solution and 3% acetic acid solution to the composition in a vacuum mixer to adjust the pH value of the solution to 6.0, adjust the reaction temperature to 45°C, add the extracted myrosinase, mix well and react for 35 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

[0184] Wherein, the extraction method of myrosinase is:

[0185] (1) The broccoli seeds were ground with liquid nitrogen to crush the cells, and then petroleum ether was added and ultrasonicated for 10 min. The mixture was then allowed to stand for 12 h to defat.

[0186] (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 20° C., removing petroleum ether, and obtaining myrosinase.

[0187] Testing and statistics:

[0188] The content of sulforaphane in the examples and comparative examples was detected by liquid chromatography (HPLC), and the detection equipment included: a high performance liquid chromatograph, an ultraviolet detector, a fluorescence detector, a centrifuge, and a micro-injector.

[0189] Specific detection methods include:

[0190] (1) adding the composition to be tested into methanol, subjecting it to ultrasonic treatment at a power of 0.5 kW and extraction for 15 min, and then centrifuging and collecting the clear liquid separated from the upper layer;

[0191] (2) Chromatographic column loading: An octadecyl chromatographic column was loaded into a liquid chromatograph, the column temperature was set at 35.5° C., and the mobile phase pump and detector were turned on;

[0192] (3) preparing the mobile phase. In this embodiment and comparative example, a mixed solution of methanol, water and 0.125% acetic acid was used, and the pH value was adjusted to about 4.0;

[0193] (4) injecting the prepared sample into a liquid chromatograph using a microsyringe, adjusting the flow rate to 2.25 mL / min, and performing liquid chromatography separation at a detection wavelength of 201 nm to separate sulforaphane;

[0194] (5) Detect the peak value of sulforaphane using an ultraviolet or fluorescence detector.

[0195] Among them, the quantitative method is the existing external standard method. By using the sulforaphane standard as a control, the content of sulforaphane in the sample can be more accurately determined. The specific method is to perform chromatographic analysis after the standard and the composition sample are treated in the same way, and the content of sulforaphane is calculated based on the peak area of ​​the standard.

[0196] The content of glucoraphanin in the composition and the content of the generated sulforaphane are recorded, and then the conversion rate of glucoraphanin is calculated, conversion rate = (sulforaphane content / glucoraphanin content) × 100%; the statistical data is made into a data table.

[0197] Table 1: Statistical table of sulforaphane content and conversion rate detected in Examples 1-7

[0198]

[0199] Based on Table 1 above, we can conclude that:

[0200] (1) From Examples 1-5, it can be concluded that the conversion rate of the composition containing papaya for improving the bioavailability of glucoraphanin can reach up to 45% under the catalysis of myrosinase;

[0201] (2) It can be concluded from Example 1 and Examples 6-7 that when papaya extract, broccoli seed water extract and vitamin C solution are mixed at a mass ratio of 20:3:1, the content of sulforaphane will increase when the concentration of the vitamin C solution increases, that is, the conversion rate will increase; and the content of sulforaphane will decrease, that is, the conversion rate will increase when the concentration of the vitamin C solution decreases.

[0202] Table 2: Statistical table of sulforaphane content and conversion rate detected in Comparative Examples 1-5

[0203]

[0204] Based on Table 2 above, we can conclude that:

[0205] When the mass ratio of broccoli seed water extract increased from 0.1:3:1 to 20:3:1, the conversion rate of glucoraphanin showed an upward trend with a slow increase rate; when the mass ratio of broccoli seed water extract increased from 20:3:1 to 50:3:1, the conversion rate of glucoraphanin remained almost unchanged and showed a stable trend.

[0206] Table 3: Statistical table of sulforaphane content and conversion rate detected in Comparative Examples 6-9

[0207]

[0208] Based on the above, we can conclude that:

[0209] (1) When the mass ratio of papaya extract increased from 20:0:1 to 20:5:1, it can be seen that the conversion rate of glucoraphanin increased with a large rate of increase;

[0210] (2) When the mass ratio of papaya extract increased from 20:5:1 to 20:10:1, it can be seen that the conversion rate of glucoraphanin gradually decreased, and the rate of decrease was small.

[0211] Table 4: Statistical table of sulforaphane content and conversion rate in comparative examples 10-13

[0212]

[0213] Based on Table 3-Table 4, we can conclude that:

[0214] (1) It can be seen from Comparative Examples 6 and 10 that when no papaya extract or vitamin C solution is added, the conversion rate of glucoraphanin in the composition is significantly reduced.

[0215] (2) When the mass proportion of vitamin C solution was increased, the conversion of glucoraphanin first increased to a maximum value of 45% and then gradually decreased.

[0216] Table 5: Statistical table of sulforaphane content and conversion rate in comparative examples 14-17

[0217]

[0218] Based on Tables 1-5 above, we can conclude that:

[0219] (1) Comparative Examples 14-17 and Examples 1, 6-7 show that when the mass ratio of papaya extract, broccoli seed water extract and vitamin C solution is 20:3:1, when the concentration of the vitamin C solution increases to 1.15 mmol / L, the conversion rate of glucoraphanin gradually increases. When the concentration of the vitamin C solution increases from 1.15 mmol / L to 1.45 mmol / L, the generation rate of glucoraphanin gradually decreases, and decreases rapidly after exceeding 1.25 mmol / L.

[0220] (2) When papaya extract and vitamin C solution were not added to the composition, the conversion rate of glucoraphanin decreased significantly.

[0221] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition containing papaya for improving the bioavailability of glucoraphanin, characterized in that: Includes broccoli seed water extract, papaya extract and vitamin C solution; The broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of (10-30):(1-5):1; Wherein, the concentration of the vitamin C solution is 1.05-1.25mmol / L.

2. The composition for improving the bioavailability of glucoraphanin containing papaya according to claim 1, characterized in that: The broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of (15-25):(2-4):

1.

3. The composition containing papaya for improving the bioavailability of glucoraphanin according to claim 2, characterized in that: The broccoli seed water extract, papaya extract and vitamin C solution are mixed in a mass ratio of 20:3:

1.

4. The composition containing papaya for improving the bioavailability of glucoraphanin according to claim 1, characterized in that: The broccoli seed water extract contains 15%-18.5% glucoraphanin.

5. The composition for improving the bioavailability of glucoraphanin containing papaya according to claim 1, characterized in that: The concentration of the vitamin C solution is 1.15 mmol / L.

6. The method for preparing the composition containing papaya for improving the bioavailability of glucoraphanin according to any one of claims 1 to 5, characterized in that: The preparation of the composition specifically comprises the following steps: S1: preparing the papaya extract; (1) Papaya seeds are crushed and sieved with liquid nitrogen, 0.04 mol / L of L-cys reagent and 0.002 mol / L of EDTA are added, 2-3 times the mass of the papaya and 60%-70% ethanol is added after mixing, the temperature is raised to 60-65° C., the pH of the mixed solution is adjusted to 5.8-6.2, and extraction is performed by immersion method; (2) taking the supernatant and concentrating it to 20%-30% of the original volume, adding it to a solvent, and then adding it to a chromatography column for elution and impurity removal, collecting the eluted components, and purifying them to obtain the papaya extract; S2: preparation of broccoli seed aqueous extract; (1) performing enzyme inactivation treatment on broccoli seeds, then pulverizing and grinding the seeds with a pulverizer, and passing the pulverized seeds through a 100-200 mesh sieve to obtain broccoli seed pulverized material; (2) adding petroleum ether to the crushed broccoli seeds to extract and defat the broccoli seeds, repeating this process 3-4 times to obtain a water extract, and extracting the supernatant after separation; (3) The supernatant is then concentrated to 20%-30% of the original volume, followed by alcohol precipitation, and finally filtered to obtain a filtrate; (4) further concentrating the filtrate to 20%-30% of the original volume, and finally obtaining the broccoli seed water extract by spray drying; S3: mixing the prepared papaya extract, broccoli seed water extract and vitamin C solution to obtain the composition; S4: Add 0.9% NaCl solution to the composition in a vacuum mixer, adjust the pH of the solution to 5-7, adjust the reaction temperature to 40-50° C., add myrosinase, mix well and react for 30-45 minutes, filter and detect the conversion rate of glucoraphanin to sulforaphane.

7. The method for preparing the composition containing papaya for improving the bioavailability of glucoraphanin according to claim 6, characterized in that: The extraction method of the myrosinase is: (1) Grind broccoli seeds with liquid nitrogen to crush cells, then add petroleum ether, apply ultrasonic treatment for 5-10 minutes, and then stand for 10-12 hours to defat; (2) placing the defatted mixed solution in a vacuum drying oven, controlling the temperature at 18-22° C., removing petroleum ether, and obtaining the myrosinase.

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