Aloe extract nano-vesicle as well as preparation method and application thereof

Abstract

The invention relates to an aloe extract nano-vesicle as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The aloe extract nano-vesicle provided by the invention is a nano-micelle formed by an aloe extract and tocopheryl acetate, the particle size of the aloe extract nano-vesicle is 280 to 500 nm, the Zeta potential of the aloe extract nano-vesicle is-10.12 to-11.34 mV, and the polydispersity coefficient of the aloe extract nano-vesicle is 0.239 to 0.434. The aloe extract nano-vesicle disclosed by the invention has a nano-scale size, and can easily penetrate through a skin barrier and enter skin cells, so that the moisturizing effect of the aloe extract nano-vesicle is achieved. The permeability of the moisturizing composition is far better than that of the traditional moisturizing composition, so that the required moisture can be more effectively provided for the skin. The aloe extract nano-vesicle can carry other active ingredients such as moisturizing factors to deeply permeate into skin, comprehensive care is provided for the skin, the texture and appearance of the skin are improved, the preparation process is simple, and the aloe extract nano-vesicle is suitable for technological production.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an aloe vera extract nanocapsule and a preparation method and application thereof. Background Art

[0002] The stratum corneum of normal skin is a complete "membrane" whose main function is to prevent water loss and protect the internal structure from damage, which is medically called "barrier function". The barrier function of dry skin is affected, making the "protective membrane" no longer complete. Therefore, the water, electrolytes and other substances in the skin will be lost through the epidermis, which will make the skin drier and form a vicious cycle; at the same time, harmful or irritating substances from the outside world can easily invade, causing skin allergies or infections, resulting in damaged barrier function and various skin problems.

[0003] Skin dehydration often leads to a variety of skin problems, such as: (1) Accelerated skin aging: Dehydration can damage collagen and elastic fibers, causing the skin to lose elasticity and firmness, making wrinkles and sagging more likely, thereby accelerating the aging process of the skin; (2) Reduced skin barrier function: A normal skin barrier can resist the invasion of harmful substances from the outside world. When there is severe dehydration, the skin barrier is damaged and is easily invaded by bacteria, viruses and allergens, causing skin inflammation and allergic reactions; (3) Affecting skin metabolism: Cell metabolism requires sufficient water to participate. Dehydration can slow down metabolism, slow down skin cell renewal, accumulate dead skin cells, and make the skin look dull and lusterless. (4) Skin diseases: It may induce or aggravate some skin diseases, such as eczema, psoriasis, and dry dermatitis, leading to uncomfortable symptoms such as itching, redness, swelling, and pain; (5) Increased skin sensitivity: Dehydration makes the skin fragile and sensitive, reduces tolerance to external stimuli such as cosmetics and climate change, and is prone to allergies and tingling; (6) Affects appearance: Dry, flaky, and rough skin can seriously affect a person's appearance and reduce self-confidence. It can be seen that skin dehydration not only affects the health of the skin, but may also lead to a decline in the quality of life. Therefore, keeping the skin well hydrated is crucial to maintaining skin health and beauty.

[0004] Skin problems caused by lack of water are not as simple as dryness. Almost every skin problem is related to water replenishment and moisturizing. Water is the first element of beautiful skin. Whitening, sun protection, anti-aging, etc. are completed on the basis of water replenishment and moisturizing. No matter the season, age, skin type, or skin problem, you can achieve perfection through the soothing and moisturizing of water.

[0005] The main contributors to keeping facial skin hydrated and moisturized are the stratum corneum and sebum membrane located on the outermost layer of the skin. When the water content in the stratum corneum reaches 15-25% (the water content of baby skin is about 25%), the face will be full of elasticity and present a healthy and clear feeling. However, as we age, unhealthy lifestyles and skin care habits will gradually lose moisture in the skin. When the water content is less than 15%, problems such as acne, dryness, fine lines, spots, oiliness, and sensitivity will occur.

[0006] Moisturizing is not simply replenishing water. Replenishing water means directly injecting water into the skin, such as applying lotion, essence, spray and mask. Moisturizing is "water replenishment + barrier repair" and locks in moisture for a long time. It forms a protective film on the skin surface to prevent the loss of water and nutrients. The advantages of moisturizing are mainly reflected in the following aspects: (1) Preventing dry skin: Moisturizing products help maintain the barrier function of the skin and prevent the loss of water and nutrients, thereby avoiding various problems caused by dry skin, such as dull skin, rough skin, dry lines and allergies; (2) Protecting skin keratin: Moisturizing products help maintain healthy keratin and sebum membrane by repairing the sebum membrane and stratum corneum, and prevent skin problems caused by damaged keratin; (3) Maintaining skin water and oil balance: Moisturizing products help maintain the water and oil balance of the skin, prevent problems such as enlarged pores and acne caused by water and oil imbalance, and thus keep the skin healthy; (3) Preventing skin aging: Maintaining skin moisture for a long time helps prevent aging phenomena such as fine lines and wrinkles, keeping the skin young and energetic. In summary, moisturizing products not only help solve existing skin problems, but also help maintain the skin's health and prevent future skin problems.

[0007] As the most basic function in skin care, moisturizing has always been the basis for the development of cosmetics. Among the external active ingredients used in domestic products, moisturizers account for the largest proportion, with a market volume of about tens of billions. Moisturizers can be divided into many types, including polyols (such as glycerin, propylene glycol, butylene glycol and sorbitol), betaine, sodium pyrrolidone carboxylate (PCA-Na), mucopolysaccharides (such as sodium hyaluronate) and panthenol (vitamin B5). These moisturizers have their own characteristics. For example, glycerin is a colorless, transparent, viscous liquid, odorless, and sweet. It is the earliest and most commonly used moisturizer in cosmetics. The performance of moisturizers can be evaluated through moisture absorption tests and moisturizing tests. In the experiment, the moisture absorption rate and moisturizing rate of moisturizers are important indicators, which can help researchers and consumers choose suitable moisturizing products.

[0008] With the increasing demand for natural cosmetics, researchers have begun to focus on natural products with biological activity, such as polysaccharides in plant extracts. Plant extracts are rich in polysaccharides, and their excellent physiological activities such as hydration, anti-oxidation, inhibition of tyrosinase activity, antibacterial, repair of skin barrier and protection against ultraviolet radiation have great potential for application in cosmetics. The development trend of cosmetic moisturizers includes the pursuit of the use of natural raw materials and the application of highly active substances. Natural ingredients such as plant polysaccharides are expected to show strong competitiveness in the cosmetics market due to their high biological activity and safety. When using moisturizers in cosmetics, in addition to considering their moisturizing effect, their safety also needs to be considered. Some moisturizers such as propylene glycol have been reported to cause skin carcinogenesis, so safety is also an important factor in choosing moisturizers. In summary, there are many types of cosmetic moisturizers, each with its own characteristics and advantages. With the increasing demand of consumers for natural and efficient moisturizing products, the cosmetics industry is also constantly exploring and developing new moisturizing ingredients to meet market demand. At the same time, the safety and efficacy of moisturizers are also important aspects that cannot be ignored in the research and development of cosmetics.

[0009] Therefore, it is particularly important to develop an ideal moisturizer, which should have moderate moisture absorption capacity, continuous moisture absorption capacity, not be easily affected by changes in environmental conditions, have moisturizing effects on the skin and the product itself, have low volatility, good coexistence with other ingredients, low freezing point, moderate viscosity, good touch, good skin affinity, high safety, and be colorless, odorless and tasteless.

[0010] There are already some moisturizing cosmetics containing aloe vera extracts, such as Chinese patents CN202410750523.1, CN202410457346.8, CN117860640A and CN117530895A, but their moisturizing and transdermal effects still need to be improved.

[0011] Some researchers have also made some improvements to aloe vera extracts. For example, Zeng Lupeng used aloe vera as the research object, isolated and extracted aloe derived nanovesicles (ADNVs) from aloe vera, and optimized the extraction method (Zeng Lupeng. Research on a new drug delivery system based on aloe vera derived nanovesicles [D]. Fujian Medical University, 2022.), but the aloe vera nanovesicles prepared are exosomes and are used as drug carriers for drug delivery. At the same time, the process for preparing aloe vera nanovesicles in this paper is ultracentrifugation, which has a high process cost and is not suitable for process production. Summary of the invention

[0012] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an aloe extract nanocapsule with significant moisture absorption and moisture retention advantages, and a preparation method and application thereof.

[0013] To achieve the above object, the technical solution adopted by the present invention is:

[0014] In a first aspect, the present invention provides an aloe extract nanovesicle, wherein the aloe extract nanovesicle is a nanomicelle formed by aloe extract and tocopherol acetate, and the aloe extract nanovesicle has a particle size of 280 to 500 nm, a Zeta potential of -10.12 to -11.34 mV, and a polydispersity coefficient of 0.239 to 0.434.

[0015] The aloe extract nanovesicles of the present invention are nanomicelles formed by the reaction of the hydroxyl groups of aloe polysaccharides in the aloe extract and the carbonyl groups of tocopherol acetate; the aloe polysaccharide molecules contain a large number of hydroxyl groups and have a rich modifiable surface, while the tocopherol acetate molecules contain free carbonyl groups, which can be used for grafting modification of aloe polysaccharides and can be prepared into nanomicelles. The nanomicelles formed by the aloe extract and tocopherol acetate can not only play a moisturizing role, but also overcome the shallow moisturizing problem that is common in traditional moisturizing with only polysaccharide extracts, and achieve deep water locking and moisturizing of the skin.

[0016] Furthermore, the mass ratio of the aloe extract to tocopherol acetate is 1:(2-6).

[0017] Preferably, when the mass ratio of the aloe extract to tocopherol acetate is 1:4, the aloe extract nanovesicle particle size is as small as 280.63nm, the Zeta potential and PDI are small, the Zeta potential is -10.64mV, and the PDI is 0.239. The aloe extract nanovesicles of this ratio can easily penetrate the skin barrier and enter the skin cells due to their nanoscale size, thereby exerting their moisturizing effect. This permeability far exceeds that of traditional moisturizing ingredients and can more effectively provide the skin with the required moisture.

[0018] In a second aspect, the present invention provides a method for preparing the aloe extract nanovesicles, comprising mixing aloe extract, tocopherol acetate and water, stirring at 50-60° C. for 2-8 hours, and performing solid-liquid separation to obtain the aloe extract nanovesicles.

[0019] In a specific embodiment of the present invention, the aloe extract nanovesicles are obtained by stirring at 50° C. for 2 to 8 hours and filtering with a 0.22 μm microporous filter membrane.

[0020] In a third aspect, the present invention provides the use of the aloe extract nanovesicles in the preparation of moisture absorbing and / or moisturizing products.

[0021] In a third aspect, the present invention provides an aloe nanocomposition, wherein the aloe nanocomposition contains the aloe extract nanovesicles and a moisturizer, wherein the moisturizer contains at least two of sodium PCA, acetyl glucosamine and hydroxyethyl urea.

[0022] Preferably, the mass ratio of the aloe vera extract nanovesicles to the moisturizing agent is (1-5):2.

[0023] More preferably, the components of the moisturizer are equal in mass.

[0024] More preferably, the moisturizer is any one of the following combinations:

[0025] Sodium PCA and acetyl glucosamine; Sodium PCA and hydroxyethyl urea; Acetyl glucosamine and hydroxyethyl urea. The composition in this formula has a synergistic effect in moisture absorption and / or moisturizing effect, significantly improving the moisture absorption and moisturizing properties of the composition.

[0026] Most preferably, when the mass ratio of the aloe extract nanovesicles, acetyl glucosamine and hydroxyethyl urea is aloe extract nanovesicles: acetyl glucosamine: hydroxyethyl urea = 5:1:1, the hygroscopicity and moisturizing effects are optimal.

[0027] In a fourth aspect, the present invention provides use of the aloe nanocomposition in preparing a moisture absorbing and / or moisturizing product.

[0028] In a fifth aspect, the present invention provides a hygroscopic and / or moisturizing product, wherein the hygroscopic and / or moisturizing product contains the aloe extract nanovesicles.

[0029] Furthermore, the hygroscopic and / or moisturizing product contains the aloe nanocomposition. The aloe extract nanovesicles of the present invention can carry other active ingredients such as moisturizing factors and penetrate deeply into the skin, provide comprehensive care for the skin, and improve the texture and appearance of the skin.

[0030] Furthermore, the content of aloe vera extract nanovesicles in the hygroscopic and / or moisturizing product is 1-4% w / w.

[0031] Furthermore, the content of the aloe nanocomposition in the hygroscopic and / or moisturizing product is 1-4% w / w, preferably 2% w / w.

[0032] Furthermore, the moisture absorbing and / or moisturizing product also contains cosmetic excipients.

[0033] Furthermore, the cosmetic auxiliary material includes at least one of a chelating agent, a moisturizing agent, a soothing agent, a thickening agent, an antioxidant, a pH regulator and a solvent.

[0034] Furthermore, the chelating agent includes disodium EDTA, the moisturizing agent includes at least one of glycerin, butylene glycol and 1,2-hexanediol, the soothing agent includes dipotassium glycyrrhizinate, the thickening agent includes carbomer, the antioxidant includes p-hydroxyacetophenone, the pH regulator includes arginine, and the solvent includes water.

[0035] Furthermore, the hygroscopic and / or moisturizing product contains, by mass, 74.22 to 91.32 parts of water, 0.05 to 0.2 parts of disodium EDTA, 2 to 6 parts of glycerol, 0.05 to 0.2 parts of dipotassium glycyrrhizinate, 0.4 to 0.8 parts of carbomer, 4 to 10 parts of butylene glycol, 0.5 to 3 parts of 1,2-hexanediol, 0.3 to 0.8 parts of p-hydroxyacetophenone, 0.38 to 0.78 parts of arginine and 1 to 4 parts of the aloe extract nanovesicles.

[0036] Furthermore, the hygroscopic and / or moisturizing product contains, by mass, 74.22 to 91.32 parts of water, 0.05 to 0.2 parts of disodium EDTA, 2 to 6 parts of glycerol, 0.05 to 0.2 parts of dipotassium glycyrrhizinate, 0.4 to 0.8 parts of carbomer, 4 to 10 parts of butylene glycol, 0.5 to 3 parts of 1,2-hexanediol, 0.3 to 0.8 parts of p-hydroxyacetophenone, 0.38 to 0.78 parts of arginine and 1 to 4 parts of the aloe nanocomposition.

[0037] Furthermore, the hygroscopic and / or moisturizing product contains, by mass, 83.6 parts of water, 0.1 parts of disodium EDTA, 4 parts of glycerol, 0.12 parts of dipotassium glycyrrhizinate, 0.6 parts of carbomer, 7 parts of butylene glycol, 1.5 parts of 1,2-hexanediol, 0.5 parts of p-hydroxyacetophenone, 0.58 parts of arginine and 2 parts of the aloe nanocomposition.

[0038] In a sixth aspect, the present invention provides a method for preparing the moisture absorbing and / or moisturizing product, comprising the following steps:

[0039] S1: Mix the components in phase A, adjust the temperature to 80-85°C, and disperse them homogeneously;

[0040] S2: After mixing and dissolving the components in phase B, mix with the product of step S1, stir evenly, and adjust the temperature to 40-45°C;

[0041] S3: Mix the components in phase C with the product of step S2, stir evenly, and adjust the temperature to room temperature;

[0042] The phase A contains at least one of water, disodium EDTA, glycerin, dipotassium glycyrrhizinate and carbomer;

[0043] The phase B contains at least one of butanediol, 1,2-hexanediol and p-hydroxyacetophenone;

[0044] The C phase contains arginine and the aloe vera extract nanovesicles.

[0045] Furthermore, the phase C contains the aloe nanocomposition.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] 1. The aloe vera extract nanovesicles and the composition of the aloe vera extract nanovesicles and other moisturizers of the present invention contain a variety of moisturizing ingredients, which can form a moisturizing film on the skin surface to lock in moisture, maintain the moisture content of the skin, and make the skin more moisturized.

[0048] 2. The aloe vera extract nanovesicles of the present invention have a nanoscale size and can easily penetrate the skin barrier and enter the skin cells to exert their moisturizing effect. This permeability far exceeds that of traditional moisturizing ingredients and can more effectively provide the skin with the required moisture.

[0049] 3. The aloe vera extract nanovesicles of the present invention have high stability and can maintain their structure and activity under different environmental conditions. This means that during the production, storage and use of cosmetics, their moisturizing effect can remain stable and is not easily destroyed.

[0050] 4. The aloe vera extract nanovesicles of the present invention have good biocompatibility and will not cause adverse reactions such as allergies and irritation. This makes the application of the aloe vera extract nanovesicles and the aloe vera extract nanovesicle composition of the present invention in moisturizing cosmetics safer and suitable for use on all skin types.

[0051] 5. The aloe vera extract nanovesicles of the present invention can carry other active ingredients such as moisturizing factors and penetrate deeply into the skin, provide comprehensive care for the skin, and improve the texture and appearance of the skin.

[0052] 6. The aloe vera extract nanovesicles and the aloe vera extract nanovesicle composition of the present invention can be applied to moisturizing products, and have the characteristics of fast absorption and long-lasting moisturizing effect. At the same time, the product can be non-clogging during long-term use and is soothing and non-irritating.

[0053] 7. The preparation process of the aloe extract nanovesicles of the present invention is simple and suitable for technological production. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 This is a sample picture of the aloe extract nanovesicles prepared in Example 1.

[0055] Figure 2 This is the particle size distribution diagram of the aloe extract nanovesicles prepared in Example 1.

[0056] Figure 3 The moisture absorption rate changes of different moisturizing materials in Example 2 under RH=43% environment.

[0057] Figure 4 The moisture absorption rate changes of different moisturizing materials in Example 2 under RH=81% environment.

[0058] Figure 5 The changes in the moisturizing rates of different moisturizing materials in Example 2 under an environment of RH=43%.

[0059] Figure 6 The moisture retention rate changes of different moisturizing raw materials in Example 2 under the dry silica gel drying environment.

[0060] Figure 7 This is a diagram showing the change in the tail area of ​​zebrafish in Example 3.

[0061] Figure 8 This is a diagram showing the change in the tail area of ​​zebrafish in Example 5. DETAILED DESCRIPTION

[0062] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific examples. Other materials, reagents, etc. used in the examples, unless otherwise specified, can be obtained from commercial sources.

[0063] The trade name of the aloe extract used in the present invention is: Aloe Extract 100:1, and the supplier is: Yunnan Wanlv Biological Co., Ltd.

[0064] Example 1

[0065] 1. The prescription of Example 1 is shown in Table 1:

[0066] Table 1 Different amounts of aloe extract and tocopherol acetate used in the nanovesicle system (by mass)

[0067]

[0068] Preparation method:

[0069] (1) Accurately weigh the formulated amount of aloe vera extract and tocopherol acetate, place them in a glass bottle, and mix them thoroughly;

[0070] (2) adding a corresponding amount of deionized water at 50° C. and stirring to dissolve the aloe extract and tocopherol acetate in step (1), and stirring for 2 to 8 hours;

[0071] (3) Filter with a 0.22 m microporous membrane to obtain aloe vera extract nanovesicles ( Figure 1 ).

[0072] Test Example 1

[0073] 1. Experimental Methods

[0074] The particle size, Zeta potential and polydispersity index (PDI) of the aloe vera extract nanovesicles in Example 1 were measured using a nanoparticle size analyzer.

[0075] 2. Experimental Results

[0076] The results are shown in Table 2. When the amount of aloe extract is 4 parts and tocopherol acetate is 1 part, the particle size of the aloe extract nanovesicles is smaller, which is 280.63nm ( Figure 2 ); Zeta potential and PDI were relatively small, with Zeta potential of -10.64 mV and PDI of 0.239, so Formulation 4 was finally selected for subsequent experiments.

[0077] Table 2 Different dosages of aloe extract and tocopherol acetate in nanovesicle system

[0078]

[0079] Test Example 2 Study on the Stability of Aloe Extract Nanovesicles

[0080] 1. Experimental Methods

[0081] The aloe extract nanovesicles of component 4 of Example 1 were stored at 4°C (cold-resistant), 25°C (normal temperature) and 45°C (heat-resistant), and were taken out after 1, 3, 5, 7, 9 and 11 days, respectively. The appearance was observed, and the particle size, Zeta potential, PDI and encapsulation efficiency of the aloe extract nanovesicles were measured to investigate the storage stability of the aloe extract nanovesicles.

[0082] 2. Experimental Results

[0083] The results are shown in Tables 3, 4 and 5. When the amount of aloe extract is 4 parts and the tocopherol acetate is 1 part, the aloe extract nanovesicles of component 4 of Example 1 are stored at 4°C (cold-resistant), 25°C (normal temperature) and 45°C (heat-resistant), respectively, and the aloe extract nanovesicles are taken out after 1, 3, 5, 7, 9 and 11 days, respectively, and the appearance is observed. The particle size, Zeta potential, PDI and encapsulation efficiency of the aloe extract nanovesicles are measured, and no obvious fluctuation is shown, which proves that they have good stability.

[0084] Table 3 Stability of Aloe Vera Extract Nanovesicles under Cold (4°C) Conditions

[0085]

[0086] Table 4 Stability of Aloe Vera Extract Nanovesicles at Room Temperature (25°C)

[0087]

[0088] Table 5 Stability of Aloe Vera Extract Nanovesicles under Heat-resistant (45°C) Conditions

[0089]

[0090] Test Example 3 Moisture absorption rate and moisturizing rate of different moisturizing raw materials

[0091] 1. Experimental Methods

[0092] (1) Hygroscopicity evaluation

[0093] Normal indoor relative humidity (RH) is generally 40% to 60%, so two relative humidities of 43% and 81% were set in the experiment to investigate the moisture absorption and moisturizing properties of the seven moisturizing raw materials prepared by formula 4 in Example 1, namely, aloe extract nanovesicles, aloe extract, sodium PCA (sodium pyrrolidone hydroxyacid), glycerol glucoside, acetyl glucosamine, trehalose and hydroxyethyl urea in these two environments.

[0094] The samples of the moisturizing raw materials to be tested were placed in a dryer until constant weight was reached. 200 mg of each sample was weighed in a weighing bottle and placed in a dryer containing a saturated solution of (NH4)2SO4 and a relative humidity of RH=81%; 200 mg of each sample was weighed in a weighing bottle and placed in another dryer containing a saturated solution of Na2CO3 and a relative humidity of RH=43%. The dryer was placed in a biochemical incubator at 25°C, and the mass of each sample was weighed at regular intervals. The experiment was repeated 3 times in parallel, and the in vitro moisture absorption rate of the moisturizing raw materials was determined and calculated according to Formula 1.

[0095] Moisture absorption rate (%) = (M2-M1) / M1×100% (Formula 1)

[0096] Where M1 is the mass of the sample before placement (g), and M2 is the mass of the sample after placement (g).

[0097] (2) Moisture Retention Evaluation

[0098] Dry the sample of the moisturizing raw material to be tested to constant weight, accurately weigh 200 mg of the sample in a weighing bottle, add 10% of the sample weight of deionized water to different samples, and place them in a dryer containing a saturated solution of Na2CO3 and a relative humidity of RH=43% and another dryer containing only dry silica gel. Place the dryer in a biochemical incubator at 25°C, weigh the mass of each sample at regular intervals, conduct 3 parallel experiments, and determine and calculate the in vitro moisturizing rate of different moisturizing raw materials according to Formula 2.

[0099] Moisture retention rate (%) = (H2-H1) / H2×100% (Formula 2)

[0100] Where H1 is the mass of water lost (g), and H2 is the mass of water contained in the sample (g).

[0101] 2. Experimental Results

[0102] The moisture absorption results are shown in Tables 6 and 7. Figure 3 and Figure 4As shown, the moisturizing rate results are shown in Tables 8 and 9 and Figure 5 and Figure 6 As shown. From the results of the in vitro moisture absorption and moisturizing evaluation of each moisturizing raw material, it can be seen that the moisture absorption and moisturizing rates of aloe vera extract nanovesicles, aloe vera extract, PCA sodium and glycerol glucoside are much higher than those of acetyl glucosamine, trehalose and hydroxyethyl urea. From the results, it can be seen that the moisture absorption and moisturizing rates of aloe vera extract nanovesicles, aloe vera extract, glycerol glucoside and PCA sodium are large, and the moisturizing effect is better; the moisture absorption and moisturizing rates of trehalose, hydroxyethyl urea and acetyl glucosamine are small, and the moisturizing effect is weak. It was also found from the results that the moisture absorption and moisturizing rates of aloe vera extract nanovesicles are better than those of aloe vera extract, indicating that the moisturizing performance of aloe vera extract nanovesicles is better than that of aloe vera extract.

[0103] Table 6 Changes in moisture absorption rate of different moisturizing materials in an environment of RH = 43%

[0104]

[0105]

[0106] Table 7 Changes in moisture absorption rate of different moisturizing materials in an environment of RH = 81%

[0107]

[0108] Table 8 Changes in moisturizing rate of different moisturizing materials under RH = 43% environment

[0109]

[0110] Table 9 Changes in moisture retention rate of different moisturizing materials under dry silica gel drying environment

[0111]

[0112] Test Example 4 Evaluation of Hygroscopicity and Moisturizing Properties of Moisturizing Raw Materials

[0113] 1. Experimental Methods

[0114] Different proportions of aloe vera extract or aloe vera extract nanovesicles are screened and compounded with moisturizing raw materials such as hydroxyethyl urea, acetyl glucosamine, glycerol glucoside, PCA sodium and trehalose, in order to obtain the most optimal ratio compound so that it can exert a better moisturizing effect and achieve a synergistic effect between the components.

[0115] In Test Example 3, based on component 4 of Example 1, it was found that glycerol glucoside, sodium PCA, hydroxyethyl urea, and acetyl glucosamine had a greater impact on the experimental results, so glycerol glucoside, sodium PCA, hydroxyethyl urea, and acetyl glucosamine were selected for compounding, and the experimental design was shown in Tables 10 and 11, and the total mass of each group remained consistent. According to the method of Test Example 3, the moisture absorption rate and the moisture retention rate were detected and calculated.

[0116] Table 10 Examples of compounding of different moisturizing raw materials (parts by mass)

[0117]

[0118] Table 11 Comparative Examples of Compounds of Different Moisturizing Raw Materials (Parts by Mass)

[0119]

[0120]

[0121] 2. Experimental Results

[0122] The results of in vitro moisture absorption and moisturizing evaluation of each composite raw material are shown in Tables 12 to 15. When aloe extract nanovesicles are added to the strong moisturizing raw material, Example 3, i.e., aloe extract nanovesicles: acetyl glucosamine: hydroxyethyl urea = 5:1:1 (mass ratio), the moisture absorption and moisturizing effects are optimal.

[0123] Table 12 Changes in moisture absorption rate of aloe vera extract nanovesicles compounded with moisturizing raw materials in an environment with RH = 43%

[0124]

[0125] Table 13 Changes in moisture absorption rate of aloe vera extract nanovesicles compounded with moisturizing raw materials in an environment with RH = 81%

[0126]

[0127]

[0128] Table 14 Changes in moisturizing rate after aloe vera extract nanovesicles were compounded with moisturizing raw materials in an environment with RH = 43%

[0129]

[0130]

[0131] Table 15 Changes in moisturizing rate of aloe vera extract nanovesicles compounded with moisturizing raw materials in dry environment

[0132]

[0133] Test Example 5 Synergistic Effect of Moisturizing Raw Material Compound in Hygroscopicity and Moisturizing Property

[0134] I. Experimental Method

[0135] From the results of Test Example 4, it is known that the effect of the aloe extract nanovesicle compound composition is better than that of the aloe extract. To explore whether there is a synergistic effect between its components, the addition principle is used to calculate the synergy coefficient to screen the existing components, in order to obtain the synergistic and antagonistic results between different compositions.

[0136] The addition rule is usually used to evaluate the expected effects of two drugs or ingredients. If two ingredients A and B have independent effects E_A and E_B respectively, then their additive effect is: E_total = E_A + E_B. If the actually observed effect E_observed is greater than E_total, there may be a synergistic effect. If the effects of two substances acting alone are A and B respectively, and their sum is A + B. When they act together, the effect is greater than A + B, then there may be a synergistic effect. For example, substance A can achieve a moisturizing effect of 30%, and substance B can achieve 40%. The sum is 70%, but when the two are used together, the moisturizing effect reaches 80%, which means there is a synergistic effect. The specific calculation rules for the theoretical hygroscopicity and moisturizing rate values of the compound composition are as follows:

[0137] The operation rules for the theoretical hygroscopicity and moisturizing rate E_total of the compound composition are shown in Equation (3).

[0138]

[0139] Where E_A is the hygroscopicity or moisturizing rate of moisturizing raw material A used alone; E_B is the hygroscopicity or moisturizing rate of moisturizing raw material B used alone; n1 is the proportion (parts by mass) of moisturizing raw material A in the compound composition; n2 is the proportion (parts by mass) of moisturizing raw material B in the compound composition; N is the total amount (parts by mass) of the composition

[0140] If the actually measured hygroscopicity and moisturizing rate of the compound composition, that is, E_observed > E_total, it proves that the composition has a synergistic effect. If the actually measured hygroscopicity and moisturizing rate of the compound composition, that is, E_observed < E_total, it proves that the composition has an antagonistic effect.

[0141] II. Experimental Results

[0142] The synergy coefficient was calculated from the in vitro hygroscopic and moisturizing evaluation results of each compound raw material, which verified that the addition of aloe vera extract nanovesicles can significantly improve the hygroscopic and moisturizing properties of the formula, and has a synergistic effect. When aloe vera extract nanovesicles are added to strong moisturizing raw materials, the hygroscopic and moisturizing effects show a significant increasing trend, and have a synergistic effect; when aloe vera extract nanovesicles are added to weak moisturizing raw materials, the hygroscopic and moisturizing effects also show a significant increasing trend, and have a synergistic effect (Tables 16 to 19). In summary, when aloe vera extract nanovesicles are added to cosmetic formulas, while ensuring that the dosage remains unchanged, the moisturizing effect of the formula can be significantly improved, achieving synergistic effects and reducing costs.

[0143] The moisture absorption synergy of the formulation in Example 2 under RH=43% is used as an example for calculation:

[0144] E_total(%)=(23.45%×1) / 3+(9.52%×1) / 3+(6.88%×1) / 3=13.28%.

[0145] Table 16 Moisture absorption synergy of aloe vera extract nanovesicles after compounding in RH = 43% environment

[0146]

[0147]

[0148] Table 17 Moisture absorption synergy of aloe extract nanovesicles after compounding in an environment of RH = 81%

[0149]

[0150] Table 18 Moisturizing synergy of aloe vera extract nanovesicles compounded in RH = 43% environment

[0151]

[0152] Table 19 Moisture retention synergy of aloe vera extract nanovesicles compounded in dry silica gel drying environment

[0153]

[0154] Test Example 6 Zebrafish test to evaluate the moisturizing properties of aloe vera extract nanovesicles

[0155] 1. Inspection basis:

[0156] BYMWKJ-3A-021 "Standard operating procedures for zebrafish moisturizing efficacy evaluation experiment".

[0157] 2. System and sample size

[0158] Translucent Albino zebrafish with melanin allele mutation or wild-type AB zebrafish.

[0159] 3. Zebrafish age

[0160] 2 days post fertilization (2dpf).

[0161] 4. Sample size of each experimental group:

[0162] 15 tails (N=10).

[0163] 5. Principle and method

[0164] When zebrafish are treated with sodium chloride, the skin surface of the zebrafish will lose water and shrink due to osmotic pressure, and the tail area will become smaller due to shrinkage. In this experiment, whether the samples (Example 3 and Example 5) have moisturizing effects is evaluated by the change in the tail area of ​​the zebrafish.

[0165] 6. Experimental steps

[0166] (1) Zebrafish with similar growth and development were randomly selected and placed in a 6-well plate, with 15 zebrafish in each well.

[0167] (2) Water-soluble administration samples, normal control group and model control group were set up at the same time, and the volume of each well was 3 mL.

[0168] (3) Sodium chloride was dissolved in water to establish a zebrafish skin dehydration model.

[0169] (4) Incubate at 28.5°C in the dark for 22 to 24 hours.

[0170] (5) Ten zebrafish were randomly selected from each experimental group and photographed under a stereo microscope. Advanced image processing software was used to analyze and collect data. The tail area (S) of the zebrafish was analyzed, and the moisturizing effect of the sample was calculated according to the following formula (Formula 4) to determine whether it had moisturizing effect.

[0171] Moisturizing effect (%) = [S (sample group) - S (model control group)] / [S (normal control group) - S (model control group)] × 100% (Formula 4)

[0172] 7. Experimental results

[0173] The test results are shown in the following table (Table 20). Figure 7 and Figure 8 As shown, they represent Example 3 and Example 5 respectively.

[0174] Table 20 Analysis of the difference in zebrafish tail area between the model control group and the sample group

[0175]

[0176] After testing, the tail area of ​​zebrafish in the sample group with a concentration of 0.5% was significantly increased compared with the model control group, revealing that the sample has a moisturizing effect. Among them, the moisturizing effect of Example 3 is 44%, showing the best moisturizing effect. Therefore, when hydroxyethyl urea: acetyl glucosamine: aloe extract nanovesicles = 1:1:5 (mass ratio), the moisturizing effect is the best.

[0177] Application Example 1

[0178] An aloe vera gel prepared by using the aloe vera nanocomposition 3 of the compound raw materials of Example 3, the components of the aloe vera gel are shown in Table 21 below:

[0179] Table 21 Aloe vera gel containing aloe vera extract nanovesicles

[0180]

[0181] Preparation method:

[0182] (1) Add water to phase A, and add the remaining components of phase A in sequence while stirring, heat to 80-85°C, and mix until fully dispersed. Keep the temperature constant for 15 minutes for later use;

[0183] (2) Preheat and dissolve phase B, then add it to phase A in step (1), stir evenly and cool to 40-45°C;

[0184] (3) Add phase C to the product obtained in step (2) after stirring evenly, stir evenly, and cool to room temperature.

[0185] Comparative Example 24

[0186] The difference between the aloe vera gel component of Comparative Example 24 and that of Application Example 1 is that the aloe vera gel component of Comparative Example 24 does not contain aloe vera extract nanovesicles, and the other preparation methods are the same.

[0187] Test Example 7: Test on the immediate repair effect of aloe vera gel on the human body

[0188] Transepidermal water loss (TEWL) is an important parameter for evaluating the barrier function of the skin. It indicates the loss of water from the deep dermis through evaporation of the epidermis. The higher the TEWL, the more water is lost through the skin and the weaker the skin's moisturizing ability. The TEWL is closely related to the hydration of the stratum corneum and is a common indicator reflecting the barrier function of the stratum corneum. In the development of cosmetics, the efficacy of moisturizing cosmetics can be evaluated by testing the TEWL value of skin water loss. In addition, the TEWL can also be used in allergic patch tests, contact dermatitis, physical therapy, burns and new tissue monitoring to promptly detect whether the skin's protective function has been destroyed. Therefore, the TEWL is an important parameter for evaluating skin health and is of great significance to the development and evaluation of moisturizing products.

[0189] 1. Experimental Methods

[0190] Fifty healthy subjects aged between 18 and 60 years old with no injuries on the inner side of their arms were selected and divided into groups, each with 25 people, including 12 males and 13 females. A sample group and a blank group (i.e., negative control group: no test sample) were set up, and the test area was randomly distributed 1 cm inside the volunteers' arms. 2 Area. The forearms (two areas) of the above-mentioned subjects were torn off with medical tape 20 times to induce damage to the skin barrier function, and then 5 mg / mL histamine was added to model for 10 minutes (obvious erythema appeared). One area was treated with 1g of the test product, and the other area was blank as a negative control area. The transepidermal water loss (TEWL) of the forearm skin was collected before tape tearing off (T0), before using the product (i.e., immediately after histamine) (T1), and 2 hours after using the product (T2). The difference in skin index changes between the product-treated area and the negative control area before and after using the product was used to evaluate the short-term repair effect of the product on skin barrier damage caused by tape tearing and histamine-induced skin irritation, and the average value was taken.

[0191] Relative water loss rate of epidermis = (test value - blank value) / test value × 100% (Formula 5)

[0192] Table 22 Relative water loss rate of epidermis

[0193]

[0194] 2. Experimental Results

[0195] According to the results analysis in Table 22: From the results of Application Example 1 and Comparative Example 24, the aloe vera gel prepared by nanovesicles containing aloe vera extract has significant moisturizing and soothing effects. When the mass fraction of the composition in Application Example 1 is 2wt%, the moisturizing and soothing effects are already very obvious, indicating that the composition can effectively improve the moisturizing and soothing effects of the aloe vera gel matrix.

[0196] Test Example 8: Consumer Experience Survey

[0197] The aloe vera gel prepared in Application Example 1 was selected for consumer research and testing, and 50 healthy subjects aged between 18 and 60 were selected, including 15 males and 35 females. The inclusion criteria were dry skin, and the test site was the entire face. The subjects used the product for 14 consecutive days, once in the morning and once in the evening, and a questionnaire survey was conducted. The final results are shown in Table 23. The subjects' satisfaction with the product was analyzed, and the satisfaction evaluation included very satisfied, relatively satisfied, and somewhat satisfied. During the experiment, the subjects had no adverse reactions, and no subjects withdrew from the test.

[0198] Table 23 Results of consumer survey on application example 1

[0199]

[0200] The results show that the product satisfaction rate is 100%, which means that the product meets the market demand and is very popular in the market.

[0201] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An aloe vera extract nanovesicle, characterized in that: The aloe vera extract nanovesicles are nanomicelles formed by aloe vera extract and tocopherol acetate. The aloe vera extract nanovesicles have a particle size of 280 to 500 nm, a Zeta potential of -10.12 to -11.34 mV, and a polydispersity coefficient of 0.239 to 0.

434.

2. The aloe vera extract nanovesicle according to claim 1, characterized in that The mass ratio of the aloe extract to tocopherol acetate is 1:(2-6).

3. The method for preparing the aloe extract nanovesicles according to claim 1 or 2, characterized in that: Aloe extract, tocopherol acetate and water are mixed, stirred at 50-60° C. for 2-8 hours, and solid-liquid separation is performed to obtain the aloe extract nanovesicles.

4. Use of the aloe extract nanovesicles according to claim 1 or 2 in the preparation of moisture absorbing and / or moisturizing products.

5. An aloe nanocomposition, characterized in that the aloe nanocomposition contains the aloe extract nanovesicles according to claim 1 or 2 and a moisturizer, wherein the moisturizer contains at least two of sodium PCA, acetyl glucosamine and hydroxyethyl urea. 6 . The aloe nanocomposition according to claim 5 , characterized in that the mass ratio of the aloe extract nanovesicles to the moisturizing agent is (1-5):

2.

7. The aloe nanocomposition according to claim 6, characterized in that the components of the moisturizing agent are equal in mass.

8. Use of the aloe nanocomposition according to any one of claims 4 to 7 in the preparation of moisture absorbing and / or moisturizing products.

9. A hygroscopic and / or moisturizing product, characterized in that: The moisture absorbing and / or moisturizing product contains the aloe vera extract nanovesicles according to claim 1 or 2.

10. The method for preparing the hygroscopic and / or moisturizing product according to claim 9, characterized in that: The following steps are involved: S1: Mix the components in phase A, adjust the temperature to 80-85°C, and disperse them homogeneously; S2: After mixing and dissolving the components in phase B, mix with the product of step S1, stir evenly, and adjust the temperature to 40-45°C; S3: Mix the components in phase C with the product of step S2, stir evenly, and adjust the temperature to room temperature; The phase A contains at least one of water, disodium EDTA, glycerin, dipotassium glycyrrhizinate and carbomer; The phase B contains at least one of butanediol, 1,2-hexanediol and p-hydroxyacetophenone; The C phase contains arginine and the aloe vera extract nanovesicles according to claim 1 or 2.

Images