An osmotic base membrane anti-aging lip care composition containing a plurality of recombinant collagens and a method of preparing the same

Abstract

The present application relates to the field of lip cosmetic technology, international classification number is A61K8, more particularly relates to a kind of containing multiple recombinant collagen's permeation type basement membrane anti-aging lip care composition and its preparation method.The present application uses multiple recombinant collagen, polyhydric alcohol, vegetable oil, penetration enhancer consisting of azone, PPG-3-lauryl alcohol polyether-12 and PEG-10 soya sterol, the plant extract prepared by decocting, enzymolysis, extracting and extracting of ganoderma extract, african mahogany bark, liquorice, snowbell, and thickener and water, jointly prepare lip composition, the composition obtained by the present application can effectively promote moisturizing and prevent lip aging, with high efficient permeability and can effectively enhance the irritability of lip resistance to external allergen or irritant source, experience effect is good, can effectively wet and moisturize lip skin, while giving lip not tight effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology for lips, with the international classification number A61 K8, and more specifically to a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens and its preparation method. Background Technology

[0002] Lip care cosmetics mainly include lip balm, lip mask, lip oil, and lip scrub, etc. The main function of these products is to nourish and repair the lips.

[0003] Lip balm typically contains oils, waxes, and pigments to moisturize and protect the lips. Lip masks are products used to deeply nourish and repair the lips; they usually contain rich nutrients and provide long-lasting hydration and repair. Lip oils are lip care products that provide moisture and shine, offering high moisturizing effects and long-lasting protection. Lip scrubs are used to remove dead skin and cuticles from the lips, helping to restore their smoothness and health; they usually contain fine particles to gently remove dead skin.

[0004] There is a wide variety of lip cosmetics available, but their quality varies greatly. Many of these cosmetics, while pursuing multiple functions, often suffer from inconsistent strength of each function. Furthermore, the use of large amounts of chemical raw materials, active ingredients, and large-molecule peptides in these cosmetics results in poor penetration and delivery to the skin. Therefore, providing a lip cosmetic that effectively promotes moisturizing and prevents lip aging, possesses high permeability, and effectively enhances the lips' resistance to irritation from external allergens or irritants is a current research topic. Summary of the Invention

[0005] To address the aforementioned technical problems, the inventors have proposed the technical solution of this invention, specifically relating to a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagen proteins and its preparation method.

[0006] This invention provides a penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens, comprising the following raw materials in parts by weight: 0.05-1 parts recombinant collagen, 8-15 parts polyol, 4-7 parts vegetable oil, 2-4 parts penetration enhancer, 0.1-2 parts plant extract, 0.1-0.8 parts thickener, and 75-100 parts water.

[0007] Preferably, the recombinant collagen includes recombinant type I humanized collagen, recombinant type III humanized collagen, recombinant type VI humanized collagen, recombinant type VI I humanized collagen, and recombinant type XVI I humanized collagen. These recombinant collagens are referred to as recombinant collagen type I, type III, type VI, type VI I, and type XVI I, respectively, in a mass ratio of (0.5-2):(0.5-2):(0.5-2):(0.5-2):(0.5-2).

[0008] Preferably, the polyol includes at least one selected from sorbitol, ethylene glycol, propylene glycol, butylene glycol, and glycerol.

[0009] Preferably, the vegetable oil includes at least one of grapeseed oil, jojoba oil, camellia oil, evening primrose oil, rapeseed oil, lemon oil, avocado oil, olive oil, almond oil, palm oil, and sweet almond oil.

[0010] Preferably, the penetration enhancer includes 0.2-0.5 parts azone, 0.9-1.2 parts PPG-3-lauryl polyether-12 and 0.9-1.3 parts PEG-10 soybean sterol.

[0011] Preferably, the method for preparing the plant extract includes obtaining it by decoction, enzymatic hydrolysis, extraction with ethanol aqueous solution, and extraction from Ganoderma lucidum extract, neem bark, licorice, and Centella asiatica.

[0012] Preferably, the method for preparing the plant extract includes:

[0013] S1: Pulverize 0.2-1 parts of Ganoderma lucidum, 0.1-0.8 parts of neem bark, 0.2-0.8 parts of licorice, and 0.2-0.9 parts of Centella asiatica according to the mass ratio. Add 8-30 times the mass of water to the pulverized material, decoct and stir at 60-80℃ for 2-6 hours, cool, and obtain product one;

[0014] S2: Add 400-700 u / g of Serratia peptides to product one, and enzymatically hydrolyze at 35-55℃ for 3-6 hours to inactivate the enzyme and obtain product two.

[0015] S3: Add anhydrous ethanol to product two, adjust the ethanol concentration to 15-35wt%, stir at 40-60℃ for 2.5-5 hours, concentrate to 0.8-1.2 times the mass of the pulverized material, and obtain product three;

[0016] S4: Add 15-25 times the mass of the extractant to product 3, extract at 40-50℃ for 3-5 hours, take the aqueous phase after extraction, concentrate and dry to obtain the plant extract.

[0017] Preferably, the extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2-3:1, wherein the oil phase is ethyl acetate and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.2-0.5%.

[0018] Preferably, the thickener includes at least one of plant gum, polyacrylate and its derivatives, maleic acid copolymer, acrylic acid copolymer, cellulose and its derivatives.

[0019] Preferably, the composition further includes an adjuvant, which includes at least one of a preservative, a chelating agent, a pigment, and a pH adjuster.

[0020] Preferably, the amount of the additive is 0.05-1 part.

[0021] The present invention also provides a method for preparing the permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens, comprising the following steps:

[0022] Step 1: Weigh the raw materials;

[0023] Step 2: Heat the vegetable oil and polyol, stir and mix to obtain the oil phase;

[0024] Step 3: Heat the penetration enhancer and water, stir and mix to obtain the aqueous phase;

[0025] Step 4: Mix the oil phase and water phase, cool, add the remaining raw materials, continue stirring, sterilize, and obtain the penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagen.

[0026] The beneficial effects of this invention are:

[0027] This invention utilizes multi-type recombinant collagen, polyols, plant oils, a penetration enhancer composed of azone, PPG-3-lauryl ether-12, and PEG-10 soy sterol, plant extracts prepared from Ganoderma lucidum extract, neem bark, licorice, and Centella asiatica through decoction, enzymatic hydrolysis, ethanol aqueous solution extraction, and extraction, along with a thickener and water, to prepare a lip composition. The composition obtained by this invention can effectively promote moisturizing, prevent lip aging, and has high permeability, effectively enhancing the lips' resistance to irritation from external allergens or irritants.

[0028] The composition prepared by this invention has a good anti-inflammatory effect, which can effectively reduce the inflammation or swelling of the lips caused by the environment or irritants, and give the lips a soothing and comfortable effect.

[0029] The composition of this invention uses PG-3-laurhoyl polyether-12 and PEG-10 soybean sterol, which can effectively promote the anti-swelling effect. The two can synergistically enhance the skin-soothing effect of the lip composition. The combination of the two can effectively promote the penetration and delivery of active ingredients to the skin, thereby giving the composition a better effect.

[0030] This invention employs decoction, enzymatic hydrolysis, ethanol-water extraction, and extraction processes to effectively enhance the anti-inflammatory effects of plant extracts. The specific enzymatic hydrolysis process used in the preparation of plant extracts enhances their anti-inflammatory effects to a certain extent, giving the composition a better skin-soothing effect. The African neem bark and centella asiatica used in this invention have a synergistic effect, further enhancing the anti-inflammatory effect of the plant extracts. This invention uses an extraction agent composed of calcium chloride aqueous solution and ethyl acetate, which effectively enhances the efficacy of the plant extracts. The choice of calcium chloride solution effectively improves the dissolution and enrichment of anti-inflammatory active ingredients, resulting in a better anti-inflammatory effect. Ethyl acetate allows the organic phase to more effectively enrich and extract impurities or oily components, leading to a higher concentration of active ingredients in the plant extract obtained after drying the aqueous phase following extraction, thus enhancing the anti-inflammatory effect of the extract.

[0031] The composition of the present invention has a good user experience, effectively moisturizing and hydrating the lips while providing a non-tight feeling. Detailed Implementation

[0032] The present invention will be described below with reference to specific embodiments, thereby making the various effects of the invention clearer. Those skilled in the art should understand that these specific embodiments are for illustrative purposes only and not for limiting the invention.

[0033] Recombinant Type I Humanized Collagen: Jiangsu Chuangjian Medical Technology Co., Ltd. Recombinant Type III Humanized Collagen: Jiangsu Chuangjian Medical Technology Co., Ltd. Recombinant Type VI Humanized Collagen: Shanghai Chunmai Biotechnology Co., Ltd. Recombinant Type VI I Humanized Collagen: Shanghai Kanglang Biotechnology Co., Ltd. Recombinant Type XVI I Humanized Collagen: Jiangsu Chuangjian Medical Technology Co., Ltd. PEG-10 Soy Sterol: Hubei Hanwei Chemical Co., Ltd.

[0034] Composition 1:

[0035] The composition is prepared as follows:

[0036] Step 1: Weigh the raw materials, by weight: 0.2 parts recombinant collagen (recombinant type I humanized collagen, recombinant type I II humanized collagen, recombinant type VI humanized collagen, recombinant type VI I humanized collagen and recombinant type XVI I humanized collagen are mixed in equal weight), 5 parts 1,2-propanediol, 5 parts glycerin, 2 parts grape seed oil, 2 parts jojoba oil, 1.5 parts camellia oil, 1 part evening primrose oil, 0.3 parts azone, 1 part PPG-3-laurhodium polyether-12 and 1.2 parts PEG-10 soybean sterol, 1.5 parts plant extracts, 0.5 parts thickener carbomer-941 and 80 parts water.

[0037] Step 2: Heat the vegetable oils (grapeseed oil, jojoba oil, camellia oil, evening primrose oil) and polyols (1,2-propanediol, glycerin) to 68°C and stir at 80 rpm for 0.5 hours to obtain the oil phase;

[0038] Step 3: Heat the penetration enhancer (azone, PPG-3-lauryl ether-12 and PEG-10 soybean sterol) and water to 50°C and stir at 70 rpm for 0.3 hours to obtain the aqueous phase;

[0039] Step 4: Mix the oil phase and water phase at 70 rpm for 0.5 hours, cool to 40°C, add the remaining raw materials, continue stirring at the same speed for 0.2 hours, sterilize, and obtain the composition.

[0040] The preparation method of the plant extracts used above is as follows:

[0041] S1: Crush 0.7 parts of Ganoderma lucidum, 0.6 parts of neem bark, 0.5 parts of licorice, and 0.6 parts of Centella asiatica. Add 20 times the weight of water to the crushed materials, decoct at 78°C and stir at 60 rpm for 4.5 hours, and cool to 40°C to obtain product one.

[0042] S2: Add 500 u / g of Saraptyl peptide enzyme to product one, hydrolyze at 45°C and stir at 60 rpm for 4.5 hours to inactivate the enzyme and obtain product two.

[0043] S3: Add anhydrous ethanol to product 2, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product 3.

[0044] S4: Add 20 times the mass of the extractant to product 3, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0045] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1. The oil phase is ethyl acetate, and the aqueous phase is a 0.3% calcium chloride aqueous solution.

[0046] Composition 2:

[0047] Step 1: Weigh the raw materials, by weight: 0.2 parts recombinant collagen (recombinant type I humanized collagen, recombinant type I II humanized collagen, recombinant type VI humanized collagen, recombinant type VI I humanized collagen and recombinant type XVI I humanized collagen are mixed in equal weight), 3 parts 1,2-propanediol, 7 parts glycerin, 1.5 parts grape seed oil, 2.5 parts jojoba oil, 1 part camellia oil, 1.5 parts evening primrose oil, 0.2 parts azone, 1.1 parts PPG-3-laurhoa polyether-12 and 1.3 parts PEG-10 soybean sterol, 1.45 parts plant extracts, 0.4 parts thickener carbomer-941 and 78.5 parts water.

[0048] Step 2: Heat the vegetable oils (grapeseed oil, jojoba oil, camellia oil, evening primrose oil) and polyols (1,2-propanediol, glycerin) to 68°C and stir at 80 rpm for 0.5 hours to obtain the oil phase;

[0049] Step 3: Heat the penetration enhancer (azone, PPG-3-lauryl ether-12 and PEG-10 soybean sterol) and water to 50°C and stir at 70 rpm for 0.3 hours to obtain the aqueous phase;

[0050] Step 4: Mix the oil phase and the water phase, cool to 40°C, add the remaining raw materials, sterilize, and obtain the composition.

[0051] The preparation method of the plant extracts used above is as follows:

[0052] S1: Crush 0.5 parts of Ganoderma lucidum, 0.7 parts of neem bark, 0.4 parts of licorice, and 0.7 parts of Centella asiatica. Add 25 times the weight of water to the crushed materials, decoct at 80°C and stir at 55 rpm for 4 hours, and cool to 40°C to obtain product one.

[0053] S2: Add 550 u / g of Serratia peptides of product one to product one, enzymatically hydrolyze at 40°C and stir at 60 rpm for 4 hours to inactivate the enzyme and obtain product two.

[0054] S3: Add anhydrous ethanol to product two, adjust the ethanol concentration to 35wt%, stir at 50℃ for 4.5 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product three;

[0055] S4: Add 25 times the mass of the extractant to product 3, extract at 45℃ for 4.2 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.11 wt%, and obtain the plant extract.

[0056] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 3:1. The oil phase is ethyl acetate, and the aqueous phase is a 0.5% calcium chloride aqueous solution.

[0057] Composition 3:

[0058] Compared to Composition 1, which does not contain 1 part PPG-3-laurhoyl polyether-12 and 1.2 parts PEG-10 soybean sterol, its amount can be made up with water. Everything else is the same as Composition 1.

[0059] Composition 4:

[0060] Compared to composition 1, PPG-3-laurhodium polyether-12 is omitted and the amount of PEG-10 soybean sterol is changed to 2.2 parts. Everything else is the same as composition 1.

[0061] Composition 5:

[0062] Compared to composition 1, PEG-10 soybean sterol is omitted and the amount of PPG-3-laurhoyl polyether-12 is changed to 2.2 parts. Everything else is the same as composition 1.

[0063] Composition 6:

[0064] The difference between this composition and composition 1 lies in the preparation method of the plant extract, specifically:

[0065] The preparation method of plant extracts is as follows:

[0066] S1: Crush 0.7 parts of Ganoderma lucidum, 0.6 parts of neem bark, 0.5 parts of licorice, and 0.6 parts of Centella asiatica. Add 20 times the weight of water to the crushed materials, decoct at 78°C and stir at 60 rpm for 4.5 hours, and cool to 40°C to obtain product one.

[0067] S3: Add anhydrous ethanol to product one, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product two.

[0068] S4: Add 20 times the mass of the extractant to product 2, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0069] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1. The oil phase is ethyl acetate, and the aqueous phase is a 0.3% calcium chloride aqueous solution.

[0070] Composition 7:

[0071] The difference between this composition and composition 1 lies in the preparation method of the plant extract, specifically:

[0072] The preparation method of plant extracts is as follows:

[0073] S1: Crush 0.7 parts of Ganoderma lucidum, 0.5 parts of licorice, and 1.2 parts of Centella asiatica, add 20 times the weight of water to the crushed materials, decoct at 78°C and stir at 60 rpm for 4.5 hours, cool to 40°C to obtain product one;

[0074] S3: Add anhydrous ethanol to product one, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product two.

[0075] S4: Add 20 times the mass of the extractant to product 2, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0076] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1. The oil phase is ethyl acetate, and the aqueous phase is a 0.3% calcium chloride aqueous solution.

[0077] Composition 8:

[0078] The difference between this composition and composition 1 lies in the preparation method of the plant extract, specifically:

[0079] The preparation method of plant extracts is as follows:

[0080] S1: Grind 0.7 parts of Ganoderma lucidum, 1.2 parts of neem bark, and 0.5 parts of licorice into powder, add 20 times the weight of water to the powder, decoct at 78°C and stir at 60 rpm for 4.5 hours, cool to 40°C to obtain product one;

[0081] S2: Add 500 u / g of Saraptyl peptide enzyme to product one, hydrolyze at 45°C and stir at 60 rpm for 4.5 hours to inactivate the enzyme and obtain product two.

[0082] S3: Add anhydrous ethanol to product 2, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product 3.

[0083] S4: Add 20 times the mass of the extractant to product 3, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0084] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1. The oil phase is ethyl acetate, and the aqueous phase is a 0.3% calcium chloride aqueous solution.

[0085] Composition 9:

[0086] The difference between this composition and composition 1 lies in the preparation method of the plant extract, specifically:

[0087] The preparation method of plant extracts is as follows:

[0088] S1: Crush 0.7 parts of Ganoderma lucidum, 0.6 parts of neem bark, 0.5 parts of licorice, and 0.6 parts of Centella asiatica. Add 20 times the weight of water to the crushed materials, decoct at 78°C and stir at 60 rpm for 4.5 hours, and cool to 40°C to obtain product one.

[0089] S2: Add 500 u / g of Saraptyl peptide enzyme to product one, hydrolyze at 45°C and stir at 60 rpm for 4.5 hours to inactivate the enzyme and obtain product two.

[0090] S3: Add anhydrous ethanol to product 2, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product 3.

[0091] S4: Add 20 times the mass of the extractant to product 3, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0092] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1, with the oil phase being ethyl acetate and the aqueous phase being water.

[0093] Composition 10:

[0094] The difference between this composition and composition 1 lies in the preparation method of the plant extract, specifically:

[0095] The preparation method of plant extracts is as follows:

[0096] S1: Crush 0.7 parts of Ganoderma lucidum, 0.6 parts of neem bark, 0.5 parts of licorice, and 0.6 parts of Centella asiatica. Add 20 times the weight of water to the crushed materials, decoct at 78°C and stir at 60 rpm for 4.5 hours, and cool to 40°C to obtain product one.

[0097] S2: Add 500 u / g of Saraptyl peptide enzyme to product one, hydrolyze at 45°C and stir at 60 rpm for 4.5 hours to inactivate the enzyme and obtain product two.

[0098] S3: Add anhydrous ethanol to product 2, adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, concentrate under reduced pressure at 50℃ to 1 times the mass of the pulverized material, and obtain product 3.

[0099] S4: Add 20 times the mass of the extractant to product 3, extract at 48℃ for 4 hours, take the aqueous phase after extraction, concentrate under reduced pressure at 58℃ to 1 times the mass of the pulverized material, freeze dry at -42℃ to a water content of 2.05wt%, and obtain the plant extract.

[0100] The extractant consists of an aqueous phase and an oil phase in a mass ratio of 2.5:1. The oil phase is cyclohexane, and the aqueous phase is a 0.3% calcium chloride aqueous solution.

[0101] Experimental test:

[0102] 1. Patch test

[0103] The above composition was diluted to a concentration of 100 mg / mL using PBS buffer at pH 7 as the test sample.

[0104] Healthy volunteers aged 30-45 years were recruited and randomly assigned to groups of 5 men and 5 women. 0.05 mL of test sample was placed in a spot tester using a syringe, and the spot test tape was applied to the volunteer's left forearm skin. Gentle pressure was applied to remove air and ensure even sample distribution. Observations were conducted 24 hours later, and skin irritation was assessed according to the evaluation criteria described in Table 1. Results are shown in Table 2.

[0105] Table 1: Evaluation Criteria

[0106]

[0107] Table 2: Test Results

[0108]

[0109]

[0110] 2. Anti-inflammatory test

[0111] Healthy SPF-grade Wistar rats weighing 230±10g were selected and acclimatized for 5 days. The rats were randomly divided into three groups: a control group, a positive drug group (positive drugs were compound dexamethasone acetate cream, 999 Piyanping, and CR Sanjiu Pharmaceutical Co., Ltd.), and three combination groups (groups 1-10), for a total of 12 groups, with 10 rats in each group. In the positive drug group, 30mg of the positive drug was applied to the right ear of the rats daily at 9:00 AM. In the combination groups, 50mg of the combination was applied to the right ear of the rats daily at 9:00 AM and 8:00 PM. In the control group, 50mg of deionized water was applied to the right ear of the rats daily at 9:00 AM. The treatment continued for 10 days, with free access to water and food. On day 11, one hour after administration at 9:00 AM, the right ear of each rat in each group was exposed, and 50μL of xylene was instilled. The left ear, which did not cause inflammation, served as a control. Half an hour after inducing inflammation, the rats were euthanized by cervical dislocation. A 6mm diameter punch was used to make circular ear patches along the auricle at the same location on both ears. Ear swelling and swelling inhibition rate were calculated, and the results are shown in Table 3. Swelling degree = Right ear weight - Left ear weight; Swelling inhibition rate / % = (Average swelling degree of the blank group - Average swelling degree of the treated group) / Average swelling degree of the blank group × 100%. The test results are shown in Table 2.

[0112] Table 2: Anti-inflammatory experimental tests

[0113]

[0114] As shown in Table 1-2, the composition prepared by the present invention has a good anti-inflammatory effect, which can effectively reduce the inflammation or swelling of the lips caused by the environment or irritants, and give the lips a soothing and comfortable effect.

[0115] According to the test results of compositions 1 and 3-5, the use of PG-3-laurhoyl polyether-12 and PEG-10 soybean sterol in the composition of the present invention can effectively promote the anti-swelling effect. The two can synergistically enhance the skin-soothing effect of the lip composition. The combination of the two can effectively promote the penetration and delivery of active ingredients to the skin, thereby giving the composition a better effect.

[0116] According to tests of compositions 1 and 6-10, the decoction, enzymatic hydrolysis, ethanol-water extraction, and extraction processes of this invention effectively promote the anti-inflammatory effect of plant extracts. Tests of compositions 1 and 6 show that the specific enzymatic hydrolysis process used in the preparation of plant extracts in this invention can enhance the anti-inflammatory effect of the extracts to a certain extent, giving the compositions a better skin-soothing effect. Tests of compositions 1 and 7-8 show that African neem bark and centella asiatica can produce a compounding effect, better enhancing the anti-inflammatory effect of the plant extracts. This invention uses an extractant composed of calcium chloride aqueous solution and ethyl acetate, which can effectively enhance the effect of plant extracts. The choice of calcium chloride solution can effectively enhance the dissolution and enrichment of anti-inflammatory active ingredients, resulting in a better anti-inflammatory effect. Ethyl acetate allows the organic phase to enrich and extract impurities or oily components to a greater extent, resulting in a higher concentration of active ingredients in the plant extract obtained after drying the aqueous phase following extraction, thus enhancing the anti-inflammatory effect of the extract.

[0117] 3. User Experience Test

[0118] Fifteen volunteers aged 35-45 were selected and divided into three groups of five. Each group applied 0.3g of the above-mentioned composition (steps 1-3) to their lips. After two hours, the volunteers rated the moisturizing and skin-feeling effects. The highest score was 10. For moisturizing, the higher the score, the more moisturized the lips were; for skin-feeling, the higher the score, the more comfortable the lips felt, without any tightness. The test results are shown in Table 4.

[0119] Table 4: User Experience Test

[0120]

[0121] According to Table 4, the composition of the present invention has a good user experience, effectively moisturizing and hydrating the lips while providing a non-tightening effect. However, composition 3 lacks PPG-3-laurhoyl ether-12 and PEG-10 soybean sterol, resulting in poor dispersion and dissolution of the active ingredients and a significant decrease in moisturizing and skin-feel effects.

[0122] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagen proteins, characterized in that, The ingredients, by weight, include the following: 0.05-1 part recombinant collagen, 8-15 parts polyol, 4-7 parts vegetable oil, 2-4 parts penetration enhancer, 0.1-2 parts plant extract, 0.1-0.8 parts thickener, and 75-100 parts water; The penetration enhancer includes 0.2-0.5 parts azone, 0.9-1.2 parts PPG-3-lauryl ether-12 and 0.9-1.3 parts PEG-10 soybean sterol; The preparation method of the plant extract includes: S1: 0.2-1 parts by weight of Ganoderma lucidum, 0.1-0.8 parts by weight of neem bark, 0.2-0.8 parts by weight of licorice, and 0.2-0.9 parts by weight of Centella asiatica are pulverized, 8-30 times the weight of water is added, and the mixture is decocted and stirred at 60-80℃ for 2-6 hours, then cooled to obtain product one; S2: 400-700 u / g of Serratia peptides of product one is added to product one, and the mixture is enzymatically hydrolyzed at 35-55℃ for 3-6 hours to inactivate the enzyme, thus obtaining product two; S3: Anhydrous ethanol is added to product two, the ethanol concentration is adjusted to 15-35 wt%, the mixture is stirred at 40-60℃ for 2.5-5 hours, and concentrated to 0.8-1.2 times the weight of the pulverized material to obtain product three; S4: Add 15-25 times the mass of the extractant to product three, extract at 40-50℃ for 3-5 hours, take the aqueous phase after extraction, concentrate and dry to obtain the plant extract; the extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2-3:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.2-0.5%.

2. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that, The recombinant collagen includes recombinant collagen types I, III, VI, VII, and XVII.

3. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that, The polyol includes at least one of sorbitol, ethylene glycol, propylene glycol, butylene glycol, and glycerin.

4. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that, Vegetable oils include at least one of the following: grapeseed oil, jojoba oil, camellia oil, evening primrose oil, rapeseed oil, lemon oil, avocado oil, olive oil, almond oil, palm oil, and sweet almond oil.

5. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that, Thickeners include at least one of plant gums, polyacrylates and their derivatives, maleic acid copolymers, acrylic acid copolymers, cellulose and their derivatives.

6. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that, It also includes additives, which include at least one of preservatives, chelating agents, pigments, and pH adjusters.

7. The penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 6, characterized in that, The dosage of the auxiliary agent is 0.05-1 part.

8. A method for preparing a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to any one of claims 1-7, characterized in that, Includes the following steps: Step 1: Weigh the raw materials; Step 2: Heat the vegetable oil and polyol, stir and mix to obtain the oil phase; Step 3: Heat the penetration enhancer and water, stir and mix to obtain the aqueous phase; Step 4: Mix the oil phase and water phase, cool, add the remaining raw materials, continue stirring, sterilize, and obtain the penetrating basement membrane anti-aging lip care composition containing multiple recombinant collagen.

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