Anti-aging active peptide compound and application thereof in skin tightening products

Abstract

The invention provides an anti-aging active peptide compound and application of the anti-aging active peptide compound in a skin tightening product. According to the invention, crucian fish skin leftovers are used as a starting material, two fish skin peptides are separated by using a composite enzymolysis process, and the two fish skin peptides are combined with aloe juice and vitamin C to prepare the anti-aging active peptide compound, which is proved to be capable of effectively promoting the proliferation activity of fibroblasts, and the anti-aging active peptide compound can be used for preparing the anti-aging active peptide compound. The composition promotes fibroblasts to secrete type I and type III collagen and improves the SOD enzyme activity of skin, and can be used for resisting skin aging.

Description

Technical Field

[0001] The present invention relates to the technical field of skin firming, and in particular to an anti-aging active peptide complex and application thereof in skin firming products. Background Art

[0002] Skin aging is considered to be a decline or deterioration of physiological functions. The causes of skin aging are usually attributed to genetic mutations, telomere abnormalities, increased protein and cell damage, increased inflammation, increased cell aging, endogenous stem cell exhaustion, and cell transmission failure. How to alleviate premature skin aging is the main research and development direction of the industry.

[0003] Bioactive peptides are common cosmetic ingredients with multiple functions: anti-oxidation, moisturizing, anti-wrinkle, whitening and anti-inflammatory, etc. They can improve skin texture, delay aging, enhance skin immunity, etc., and are widely used in high-end functional cosmetics. However, there is currently a lack of effective active peptide complexes for skin anti-aging and their preparation methods. Summary of the invention

[0004] In view of the technical problems existing in the prior art, the present invention provides an anti-aging active peptide complex and its application in skin firming products. The present invention uses crucian carp skin scraps as starting materials, separates two fish skin peptides by a composite enzymatic hydrolysis process, and uses aloe vera juice and vitamin C to prepare an anti-aging active peptide complex. It has been verified that the anti-aging active peptide complex can effectively promote the proliferation activity of fibroblasts, promote the secretion of type I and type III collagen by fibroblasts and increase the activity of skin SOD enzyme, and can be used to resist skin aging.

[0005] Specifically, the present invention first provides an anti-aging active peptide complex, which includes, by weight: 10-20 parts of fish skin peptide, 5-10 parts of aloe vera, 15-20 parts of glycerin, 0.2-0.5 parts of vitamin C and 20-50 parts of ionized water.

[0006] Preferably, the anti-aging active peptide complex comprises, by weight: 10 parts of fish skin peptide, 5 parts of aloe juice, 15 parts of glycerin, 0.2 parts of vitamin C and 20 parts of deionized water; wherein the fish skin peptide sequence is SEQ ID NO.1.

[0007] Preferably, the anti-aging active peptide complex comprises, by weight: 15 parts of fish skin peptide, 8 parts of aloe juice, 16 parts of glycerin, 0.4 parts of vitamin C and 40 parts of ionized water; wherein the fish skin peptide sequence is SEQ ID NO.2.

[0008] Preferably, the anti-aging active peptide complex comprises, in parts by mass: 20 parts of fish skin peptide, 10 parts of aloe juice, 20 parts of glycerin, 0.5 parts of vitamin C and 50 parts of ionized water; wherein the fish skin peptide sequence is a combination of SEQ ID NO.1 and 2, and the mass ratio of the two is 1:1.

[0009] Preferably, the fish skin peptide is isolated from crucian carp skin waste.

[0010] Preferably, the method for separating the fish skin peptide is to use a composite enzyme consisting of 100U neutral protease and 80U trypsin to perform enzymatic separation and collect 2000-4000Da polypeptide molecules, and then separate and purify them by HPLC.

[0011] Preferably, a total of two fish skin peptides are isolated and obtained, and their sequences are shown in SEQ ID NO.1-2.

[0012] On the other hand, the present invention also provides a method for preparing an anti-aging active peptide complex, comprising the following steps:

[0013] 1) Take deionized water in portions into a container, add aloe vera juice and glycerin in sequence, and stir continuously in a water bath at 25-38°C to fully dissolve them;

[0014] 2) Add fish skin peptide and vitamin C to step 1) in sequence, and continue stirring in a 20-25° C. water bath environment to fully dissolve them.

[0015] Preferably, the method comprises the following steps:

[0016] 1) Take deionized water in portions into a container, add aloe vera juice and glycerin in sequence, and stir continuously in a 25°C water bath to fully dissolve them;

[0017] 2) Add fish skin peptide and vitamin C to step 1) in sequence, and continue stirring in a 20° C. water bath to fully dissolve them.

[0018] Preferably, the method comprises the following steps:

[0019] 1) Take deionized water in portions into a container, add aloe vera juice and glycerin in sequence, and stir continuously in a 38°C water bath to fully dissolve them;

[0020] 2) Add fish skin peptide and vitamin C to step 1) in sequence, and continue stirring in a 25° C. water bath to fully dissolve them.

[0021] On the other hand, the present invention also provides an application of an anti-aging active peptide complex in the preparation of a skin firming product.

[0022] Preferably, the anti-aging active peptide complex achieves the purpose of skin anti-aging by promoting the regeneration of skin fibroblasts, improving the antioxidant capacity of skin cells, and improving the activity of SOD enzyme.

[0023] The advantages of the present invention are as follows: the present invention uses crucian carp skin scraps as starting materials, uses a composite enzymatic hydrolysis process to separate two fish skin peptides, and combines them with aloe juice and vitamin C to prepare an anti-aging active peptide complex. It has been verified that the anti-aging active peptide complex can effectively promote the proliferation activity of fibroblasts, promote the secretion of type I and type III collagen by fibroblasts and improve the activity of skin SOD enzyme, and can be used to resist skin aging. DETAILED DESCRIPTION

[0024] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0025] The following embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. Based on the specific embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work belong to the protection scope of the present invention.

[0026] In the examples of the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art; in the examples of the present invention, unless otherwise specified, the technical means used are conventional means well known to those skilled in the art.

[0027] Example 1

[0028] An anti-aging active peptide complex comprises: 10 parts of fish skin peptide, 5 parts of aloe juice, 15 parts of glycerin, 0.2 parts of vitamin C and 20 parts of deionized water; wherein the fish skin peptide sequence is SEQ ID NO.1.

[0029] The fish skin peptide is separated from the scraps of crucian carp skin. The method for separating the fish skin peptide is to add a composite enzyme consisting of 100U neutral protease and 80U trypsin to each kilogram of crucian carp skin, make up to 1L volume with deionized water, control the conditions at 35°C, and continue stirring for 12 hours of enzymolysis. After enzymolysis, separate and collect 2000-4000Da polypeptide molecules, and separate and purify them by HPLC. A total of two fish skin peptides were separated and obtained, and the sequences are shown in SEQ ID NO.1-2.

[0030] The preparation method of the anti-aging active peptide complex comprises the following steps:

[0031] 1) Take deionized water in portions into a container, add aloe vera juice and glycerin in sequence, and stir continuously in a 25°C water bath to fully dissolve them;

[0032] 2) Add fish skin peptide and vitamin C to step 1) in sequence, and continue stirring in a 20° C. water bath to fully dissolve them.

[0033] Example 2

[0034] An anti-aging active peptide complex comprises, by weight: 15 parts of fish skin peptide, 8 parts of aloe juice, 16 parts of glycerin, 0.4 parts of vitamin C and 40 parts of ionized water. The sequence of the fish skin peptide is SEQ ID NO.2.

[0035] Example 3

[0036] An anti-aging active peptide complex comprises, by weight: 20 parts of fish skin peptide, 10 parts of aloe juice, 20 parts of glycerin, 0.5 parts of vitamin C and 50 parts of ionized water. The fish skin peptide sequence is a combination of SEQ ID NO. 1 and 2, and the mass ratio of the two is 1:1.

[0037] Comparative Example

[0038] The preparation steps of the comparative example are the same as those of Example 1, except that the anti-aging active peptide complex does not contain fish skin peptide.

[0039] Test example

[0040] Effect of anti-aging active peptide complex on fibroblast proliferation: The MTT method was used to detect the effects of the stem cell active factor essence of the examples and comparative examples on the proliferation of fibroblast HFF-1. The results are shown in Table 1. The specific detection steps can be routinely adjusted according to the instructions of the kit, wherein the blank group is DMEM complete medium, the experimental groups 1-3 respectively contain 15 μg / ml of the anti-aging active peptide complex of Examples 1-3 of the present invention, and the control group 1 is DMEM complete medium containing 15 μg / ml of the complex of the comparative example. The amount of cells in the experimental group and the control group is 1×10 5 indivual.

[0041] Table 1 Fibroblast proliferation analysis

[0042] Group OD value Blank group 0.202±0.015 Experimental Group 1 0.759±0.231 Experimental Group 2 0.689±0.051 Experimental Group 3 2.136±0.365 Control group 1 0.328±0.068

[0043] The test results are shown in Table 1: the blank group cannot effectively promote the proliferation of fibroblasts, and its proliferation ability is limited, while the anti-aging active peptide complex of the experimental group of the present invention can efficiently promote the proliferation of fibroblasts, and its proliferation promoting effect is significantly better than that of the control group (P < 0.05), the difference is significant and statistically significant.

[0044] Effect of anti-aging active peptide complex on collagen secretion of fibroblasts: According to the above grouping, the treated fibroblasts HFF-1 were cultured, and after 24 hours of culture, the cells were collected, and the secretion of type I and type III collagen was detected by ELISA kit, and superoxide dismutase was detected by xanthine oxidase method. The results are shown in Table 2.

[0045] Table 2 Type I and Type III collagen secretion

[0046]

[0047]

[0048] From the results in Table 2, it can be seen that the anti-aging active peptide complex of the present invention can efficiently promote fibroblasts to secrete type I and type III collagen and effectively increase SOD enzyme activity, and the effect is significantly better than that of the control group (P < 0.05), with significant differences and statistical significance.

[0049] It is necessary to point out that the above embodiments are limited to further elaboration and explanation of the technical solution of the present invention, and are not further limitations of the technical solution of the present invention. The method of the present invention is only a preferred implementation scheme, and is not used to limit the protection scope of the present invention. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An anti-aging active peptide complex, characterized in that: In terms of mass, it comprises: 10-20 parts of fish skin peptide, 5-10 parts of aloe vera, 15-20 parts of glycerin, 0.2-0.5 parts of vitamin C and 20-50 parts of ion water.

2. The composite according to claim 1, characterized in that In parts by mass, it comprises: 10 parts of fish skin peptide, 5 parts of aloe juice, 15 parts of glycerin, 0.2 parts of vitamin C and 20 parts of deionized water; wherein the sequence of the fish skin peptide is SEQ ID NO.

1.

3. The composite according to claim 1, characterized in that In terms of weight, it comprises: 15 parts of fish skin peptide, 8 parts of aloe juice, 16 parts of glycerin, 0.4 parts of vitamin C and 40 parts of ionized water; wherein the sequence of the fish skin peptide is SEQ ID NO.

2.

4. The composite according to claim 1, characterized in that In terms of mass, it includes: 20 parts of fish skin peptide, 10 parts of aloe juice, 20 parts of glycerin, 0.5 parts of vitamin C and 50 parts of ionized water; wherein the fish skin peptide sequence is a combination of SEQ ID NO.1 and 2, and the mass ratio of the two is 1:

1.

5. The composite according to any one of claims 1 to 4, characterized in that The fish skin peptide is separated from crucian carp skin waste.

6. The composite according to claim 5, characterized in that The method for separating the fish skin peptide is to use a composite enzyme composed of 100U neutral protease and 80U trypsin to perform enzymatic separation and collect 2000-4000Da polypeptide molecules, and then separate and purify the peptide by HPLC.

7. The composite according to claim 7, characterized in that A total of two fish skin peptides were isolated and obtained, and their sequences are shown in SEQ ID NO.1-2.

8. The method for preparing the anti-aging active peptide complex according to any one of claims 1 to 7, characterized in that: The steps include: 1) Take deionized water in portions into a container, add aloe vera juice and glycerin in sequence, and stir continuously in a water bath at 25-38°C to fully dissolve them; 2) Add fish skin peptide and vitamin C to step 1) in sequence, and continue stirring in a 20-25° C. water bath to fully dissolve them.

9. Use of the anti-aging active peptide complex according to any one of claims 1 to 7 in the preparation of skin firming products.

10. The use according to claim 9, characterized in that The anti-aging active peptide complex achieves skin anti-aging by promoting the regeneration of skin fibroblasts, improving the antioxidant capacity of skin cells, and improving the activity of SOD enzyme.

Images