Application of melatonin in preparation of medicine for promoting growth of hair follicles of young yaks and enhancing oxidation resistance

Abstract

The invention provides application of melatonin in preparation of a medicine for promoting hair follicle development of young yaks and enhancing oxidation resistance, and belongs to the technical field of application of melatonin. The research shows that the melatonin implanted in the young yak can effectively promote the fineness of hair of the young yak and the development of secondary hair follicles and enhance the immune function of the young yak. Scientific basis and technical support are provided for promoting yak hair production.

Description

Technical Field

[0001] The invention relates to the technical field of application of melatonin, and in particular to the application of melatonin in preparing a drug for promoting hair follicle development of young yaks and enhancing antioxidant capacity. Background Art

[0002] Yak (Bos grunniens) is a livestock unique to the Qinghai-Tibet Plateau. It is famous for its fine, soft, high-strength, good moisture absorption, breathability and thermal insulation properties of its hair, which is a precious animal fiber resource. These characteristics give yak hair a high economic value and are also an important source of economic income for local herders. However, due to the harsh natural environment and limited feeding and management level, the output and quality of yak hair often cannot meet market demand. Therefore, exploring effective methods to improve the output and quality of yak hair is of great significance to promoting the sustainable development of yak breeding industry.

[0003] Melatonin is a hormone secreted by the pineal gland, which is widely involved in regulating the circadian rhythm, reproductive cycle, immune function and anti-oxidative stress of organisms. In animal husbandry, melatonin has been proven to promote the production and quality of wool of animals such as cashmere goats, rex rabbits, blue foxes, rabbits, silver foxes and alpacas.

[0004] Currently, most of the research on the effects of melatonin on hair follicle development and hair characteristics of fur-bearing animals is focused on adult cashmere goats, with very little research on yaks and even no research on young yaks. Summary of the invention

[0005] In view of this, the purpose of the present invention is to explore the effect of implanting melatonin on the development of hair follicles and villi of young yaks. To this end, the present invention provides the use of melatonin in the preparation of drugs for promoting the development of hair follicles of young yaks and enhancing antioxidant capacity. Studies have shown that implanting melatonin in young yaks can effectively promote the fineness of the villi and the development of secondary hair follicles of yaks aged 2-6 months, and enhance the immune function of young yaks. This provides a scientific basis and technical support for promoting the production of yak villi.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] Application of melatonin in the preparation of drugs for promoting hair follicle development and enhancing antioxidant capacity in young yaks.

[0008] Preferably, the young yaks are 2-6 month old yaks.

[0009] Preferably, the effective dose of melatonin is 20-50 mg / kg BW, more preferably 30 mg / kg BW.

[0010] Preferably, the medicament comprises an effective dose of melatonin and a pharmaceutically acceptable adjuvant.

[0011] Preferably, the drug is a melatonin sustained-release capsule or a stick-shaped solid.

[0012] The present invention aims to explore the effect of melatonin implantation on the development of hair follicles and villi of young yaks. Healthy young yaks of about 2 months old were selected as test subjects. The effect of melatonin implantation on the length, fineness, follicle density and serum trace element content of yak villi was analyzed, and the possible mechanism of action of melatonin was explored, so as to provide theoretical basis and practical guidance for the scientific management of yak breeding industry and the improvement of villi quality. Compared with the prior art, the present invention has the following beneficial effects:

[0013] The present study showed that melatonin implantation tended to increase the density of secondary hair follicles in the skin of young yaks, and also significantly increased the ratio of secondary hair follicles to primary hair follicles (S:P), which indicated that melatonin may promote the development of secondary hair follicles, thereby improving the yield and quality of villi.

[0014] The levels of copper, magnesium, calcium and zinc in the serum of young yaks implanted with melatonin increased significantly, indicating that melatonin may affect the trace element metabolism and immune function of yaks.

[0015] The improvement of the total antioxidant capacity in the serum of the present invention further supports the role of melatonin in enhancing the antioxidant capacity of the body.

[0016] The present invention implants melatonin into young yaks, which can effectively promote the fineness of young yak hair and the development of secondary hair follicles, and enhance the immune function of young yaks, thus providing scientific basis and technical support for yak hair production. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The effects of melatonin implantation on the hair characteristics of young yaks. A is the effect of melatonin implantation on the weight of young yaks; B is the effect of melatonin implantation on the weight of young yaks' hair; C is the effect of melatonin implantation on the length of young yaks' hair; D is the effect of melatonin implantation on the fineness of young yaks' hair.

[0018] Figure 2 The effects of melatonin implantation on the characteristics of primary and secondary hair follicles in young yaks. A is the effect of melatonin implantation on the hair follicle density of young yaks; B is the effect of melatonin implantation on the ratio of secondary hair follicle density to primary hair follicle density in young yaks.

[0019] Figure 3The effects of implanted melatonin on serum melatonin and metal ion concentrations in young yaks. A is the effect of implanted melatonin on the copper content in serum of young yaks; B is the effect of implanted melatonin on the magnesium content in serum of young yaks; C is the effect of implanted melatonin on the calcium content in serum of young yaks; D is the effect of implanted melatonin on the zinc content in serum of young yaks; E is the effect of implanted melatonin on the iron content in serum of young yaks; F is the effect of implanted melatonin on the melatonin content in serum of young yaks.

[0020] Figure 4 To investigate the effect of implanted melatonin on the total antioxidant capacity of serum of young yaks. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the specific implementation described is a part of the implementation of the present invention, not all the implementations. Based on the implementation of the present invention, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] The present invention aims to explore the effect of melatonin implantation on the hair follicle development and villi characteristics of young yaks. Healthy young yaks aged about 2-6 months were selected as test subjects. The effect of melatonin implantation on the length, fineness, hair follicle density and serum trace element content of yaks was analyzed, and the possible mechanism of action of melatonin was explored, so as to provide theoretical basis and practical guidance for the scientific management of yak breeding industry and the improvement of villi quality. The specific process is as follows:

[0023] 1 Materials and methods

[0024] 1.1 Experimental Animals The experiment used young yaks about 2 months old, which were selected from local yaks in Nagqu Senyi District and provided by the ranch of Nagqu Garde Company in Tibet. The experimental period was 3 months, starting from May 2023 and ending in August 2023. 20 healthy young yaks about 2 months old (weight 19.60±0.09) kg were selected and randomly divided into a control group and an experimental group, with 10 in each group, and each young yak was a replicate. The young yaks in the control group were not treated in any way, and melatonin was implanted subcutaneously in the neck of the young yaks in the experimental group at a dose of 30 mg / kg BW. Three months after implantation, hair, wool and skin samples were collected from the right side of the hind hip of the young yaks, and venous blood was collected from the tip of the tail. The collected samples were quickly frozen and stored in liquid nitrogen at -80°C. The melatonin implant is a white rod-shaped solid (donated by the Institute of Special Products, Chinese Academy of Agricultural Sciences). You can also choose melatonin sustained-release capsules (such as melatonin sustained-release capsules) as needed. Sustained-release capsules are only a carrier of melatonin. You can also choose other harmless carriers. There are no special requirements for this. You can choose according to actual needs.

[0025] 1.2 Sample collection and determination

[0026] 1.2.1 Length and fineness of yak wool

[0027] The yak hair was cut off close to the skin (5 cm × 5 cm) on the right side of the hind hip of the young yak in the experiment, and the yak wool and yak hair were separated. The yak wool was washed, dried and weighed, and the length and fineness of the yak wool were measured. The straight length of the yak wool was measured by the steel plate ruler method (measuring 100 strands); the fineness of the wool was measured by the CU-6 fiber fineness meter (Beijing Hezhong Vision Technology Co., Ltd., measuring 200 strands).

[0028] 1.2.2 Skin samples

[0029] The hairy part on the right side of the hind hip of the experimental young yaks was cut off and shaved, then the part was cleaned, disinfected with iodine tincture, and deiodinated with alcohol. Two pieces of skin tissue were collected using a skin collector with a diameter of 8 mm, washed in 1×PBS buffer, and immediately placed in a freshly prepared 4% paraformaldehyde solution for fixation for 24 hours, stored at room temperature, and then paraffin sections were made, HE stained, dehydrated, and sealed. After drying, the primary hair follicle density and secondary hair follicle density were observed under a microscope, and the ratio of the number of secondary hair follicles to primary hair follicles (S:P) was calculated.

[0030] 1.2.3 Blood samples and measurement indicators

[0031] At the end of the experiment, about 5 ml of venous blood was collected from the tail of the yaks in the control group and the experimental group, respectively, and the blood was kept at room temperature for 30 minutes, centrifuged at 2000×g for 15 minutes, and the supernatant was taken and stored at -20°C. The following indicators were measured: blood calcium (Ca), blood zinc (Zn), blood copper (Cu), and blood magnesium (Mg) levels were measured by visible spectrophotometer; blood iron (Fe) content was measured by semi-automatic biochemical analyzer; bovine melatonin (MT) was measured by ELISA; and total antioxidant capacity (T-AOC) was measured by biochemical analyzer.

[0032] 2.3 Statistical analysis

[0033] The experimental data were preliminarily sorted using Excel 2016, and all data were plotted using OriginPro2024 software to plot the number of hair follicles, villus characteristics, and blood-related measurement bar graphs for the melatonin implantation group and the control group. The data are presented as mean and standard error.

[0034] 2 Results

[0035] 2.1 Effect of melatonin implantation on wool production in young yaks

[0036] like Figure 1 As shown in the figure, compared with the control group, the average weight of young yaks in the melatonin implantation group increased by 4.36%, the average length of villi increased by 5.62%, and the average weight of villi per unit area increased by 2.64%, but the differences were not significant (p>0.05, Figure 1 The average wool fineness of young yaks decreased by 3.88%, and the difference was significant (p≤0.05, Figure 1 D).

[0037] 2.2 Analysis of hair follicle density in young yaks

[0038] like Figure 2 As shown in the figure, compared with the control group, the primary hair follicles of the young yaks in the melatonin implantation group were intact, with a 24.2% decrease in density, and the secondary hair follicles were intact, with a 14.4% increase in density, but the difference was not significant (p>0.05, Figure 2 A); the ratio of secondary hair follicle density to primary hair follicle density increased by 117%, and the difference was significant (p≤0.5, Figure 2 B).

[0039] 2.3 Analysis of melatonin and metal ion content in plasma of young calves

[0040] like Figure 3 As shown in Figure 2, compared with the control group, the copper concentration in the serum of young yaks in the melatonin implantation group increased by 38.7%, and the difference was extremely significant (p≤0.001, Figure 3A); the magnesium content and calcium content increased by 21.4% and 16.3%, respectively, and the difference was significant (p≤0.05, Figure 3 B. Figure 3 C); the zinc content increased by 30.7%, and the difference was extremely significant (p≤0.01, Figure 3 D). The iron content and melatonin content showed an upward trend, increasing by 7.0% and 11.0% respectively, but the difference was not significant (p>0.05, Figure 3 E, 3F);

[0041] like Figure 4 As shown, compared with the control group, the total antioxidant capacity in the serum of young yaks in the melatonin implantation group increased by 9.00%, and the difference was significant (p≤0.05).

[0042] In summary, the application of melatonin provided by the present invention in the preparation of drugs for promoting the development of hair follicles in young yaks and enhancing the antioxidant capacity, the implantation of melatonin in young yaks has no significant effect on the growth performance of yaks aged 2-6 months, but can effectively promote the fineness of young yak hair and the development of secondary hair follicles, and enhance the immune function of young yaks. This provides scientific basis and technical support for the production of yak hair.

[0043] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments can still be modified, or some or all of the technical features can be replaced by equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Application of melatonin in the preparation of drugs for promoting hair follicle development and enhancing antioxidant capacity in young yaks.

2. The use according to claim 1, characterized in that: Young yaks are yaks between 2 and 6 months old.

3. The use according to claim 1, characterized in that: The effective dose of melatonin is 20-50 mg / kg BW.

4. The use according to claim 1, characterized in that: The medicine comprises an effective dose of melatonin and a pharmaceutically acceptable auxiliary agent.

5. The use according to any one of claims 1 to 4, characterized in that: The drug is a melatonin sustained-release capsule or solid stick.

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