Efficient sterilization mild laundry detergent and preparation method thereof

Abstract

The invention discloses an efficient sterilization mild laundry detergent and a preparation method thereof, and relates to the technical field of detergents. The neutral cellulase, the bromelain, the lipase and the alkaline protease are used for preparing the compound enzyme, holes and capillaries in fabric fibers are enlarged under the combined action of endonuclease and exonuclease in the neutral cellulase, stains mingled among the fabric fibers can be in contact with the bromelain, the lipase and the alkaline protease more easily, and the fabric fibers can be washed more easily. Then citric acid and acetic acid are used for providing a weak acid system for a washing environment, a clothes care effect is achieved, calcium and magnesium ions in hard water cannot be deposited, and the activity of cellulase can be improved, so that the decontamination capability and the antibacterial property of the laundry detergent are enhanced; wherein the lipase and the alkaline protease are wrapped in the supramolecular microspheres, so that the lipase and the alkaline protease are not interfered in the weakly acidic laundry detergent, and the dirt-removing power of the laundry detergent is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of detergents, in particular to a highly efficient bactericidal mild laundry detergent and a preparation method thereof. Background Art

[0002] Most of the laundry detergents on the market are washing powder or ordinary laundry liquid. Washing powder generally contains aluminum, phosphate polymers and a large amount of fillers, which not only wastes resources, but also causes eutrophication of water bodies and pollutes the environment. Washing powder has poor water solubility and is inconvenient to use. There are insoluble deposits on the surface of clothes after washing. Frequent use can easily cause ash to accumulate on clothes, making them dark and hard. In recent years, the liquid detergent industry has developed rapidly, and laundry liquid has long replaced washing powder in large quantities, becoming the main force for washing fabrics in most households.

[0003] There are a lot of stains on dirty clothes. The daily contact objects of human body such as food, blood, dairy products, sweat, grass, cosmetics, human secretions and dandruff contain a lot of protein. These proteins adhere to the fabric to form dirt, and expand and dry on the fiber over a large area. Over time, they will become gelatinous denatured proteins with poor water solubility and extremely difficult to wash off. In addition, these protein dirt can also bind other dust and granular dirt, promote the reproduction of bacteria, mold and other dirt microorganisms, produce new dirt, cause the fabric to change color and harden, and deteriorate the fiber performance. These special stains are difficult to remove with ordinary laundry detergent; and with the increasing requirements of people for environmental protection, high efficiency, no hand damage, and multi-functional washing, mild and efficient liquid detergents have become the future development direction of the washing industry. Summary of the invention

[0004] The object of the present invention is to provide a highly efficient bactericidal mild laundry detergent and a preparation method thereof, so as to solve the problems existing in the prior art.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: a highly effective bactericidal mild laundry detergent, wherein the highly effective bactericidal mild laundry detergent is prepared from a complex enzyme, a surfactant, an enzyme stabilizer and other additives.

[0006] Furthermore, the complex enzyme consists of neutral cellulase, bromelain, lipase and alkaline protease.

[0007] Furthermore, a method for preparing a highly efficient bactericidal mild laundry detergent comprises the following preparation steps:

[0008] (1) A 4.7 wt% triblock copolymer aqueous solution, lipase, and alkaline protease were mixed, stirred at 50-100 rpm for 20-40 min, a 2 wt% β-cyclodextrin aqueous solution was added, and stirring was continued for 10-15 h, and then centrifuged, the supernatant was discarded, and the remaining part was washed with deionized water for 2-5 times, and freeze-dried to obtain intermediate A;

[0009] (2) The deionized water is heated to 50-75° C., a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 2000-4000 rpm for 30-80 min. The mixture is cooled to 25-40° C., intermediate A, neutral cellulase, and bromelain are added, and the mixture is stirred at 50-100 rpm for 40-90 min to obtain a highly effective bactericidal and mild laundry detergent.

[0010] Furthermore, the triblock copolymer in step (1) is specifically a polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer.

[0011] Furthermore, the centrifugal speed in step (1) is 10000-15000 rpm and the time is 15-40 min.

[0012] Furthermore, the freeze-drying temperature of step (1) is -20 to -35°C and the time is 18 to 30 hours.

[0013] Furthermore, in step (1), the mass ratio of the 4.7 wt % triblock copolymer aqueous solution, lipase, alkaline protease, and 2 wt % β-cyclodextrin aqueous solution is 10:0.05-0.1:0.1:80-100.

[0014] Furthermore, the surfactant in step (2) is prepared by compounding soybean lecithin and fatty alcohol polyoxyethylene ether in a mass ratio of 1:1.

[0015] Furthermore, the auxiliary agent in step (2) is prepared by mixing acetic acid, citric acid and para-chloro-meta-xylenol in a mass ratio of 5:1:0.5.

[0016] Furthermore, in step (2), the mass ratio of deionized water, surfactant, auxiliary agent, 4-formylphenylboric acid, intermediate A, neutral cellulase and bromelain is 50-100:20-50:4-8:0.5-5:1:1:0.5.

[0017] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: the present invention utilizes neutral cellulase, bromelain, lipase and alkaline protease to prepare a composite enzyme, wherein the neutral cellulase first acts on the non-crystalline region on the surface of the fabric, and under the joint action of the endo- and exo-enzymes, the cavities and capillaries in the fabric fibers are enlarged, so that the fabric structure becomes fluffy, thereby making it easier for the stains interspersed between the fabric fibers to contact with the bromelain, lipase and alkaline protease, so that the stubborn stains embedded in the fibers are better removed, and then, a weakly acidic environment is provided by citric acid and acetic acid. The system is consistent with the pH value of human skin, and has the effect of softening and caring for clothes, thereby achieving mildness. At the same time, the calcium and magnesium ions in hard water will not be deposited, thereby not weakening the washing ability, and the activity of cellulase is improved in a weakly acidic environment, thereby enhancing the detergency and antibacterial properties of the laundry detergent. The lipase and alkaline protease are encapsulated in a rigid-flexible supramolecular hollow microsphere system constructed by cyclodextrin and triblock copolymers, so that the two enzymes are not disturbed in the weakly acidic laundry detergent, and the activity of the alkaline protease is protected, thereby enhancing the detergency of the laundry detergent. DETAILED DESCRIPTION

[0018] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0019] In order to more clearly illustrate the method provided by the present invention, the following examples are used to describe in detail the various index testing methods of the highly effective bactericidal mild laundry detergent prepared in the following examples are as follows:

[0020] Detergent effect: Take the same size of the embodiment and the comparative example and refer to GB / T13174, use three national standard dirty cloths of carbon black, protein and sebum, and the test concentration of laundry detergent is 0.1wt% to detect the decontamination ratio.

[0021] Example 1

[0022] (1) A 4.7 wt% aqueous solution of polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, lipase, and alkaline protease were mixed, stirred at 50 rpm for 20 min, a 2 wt% aqueous solution of β-cyclodextrin was added, and stirring was continued for 10 h, followed by centrifugation at a speed of 10,000 rpm for 15 min, the supernatant was discarded, the remaining portion was washed twice with deionized water, and freeze-dried at a temperature of -20°C for 18 h to obtain an intermediate A; the mass ratio of the 4.7 wt% aqueous solution of the triblock copolymer, lipase, alkaline protease, and 2 wt% aqueous solution of β-cyclodextrin was 10:0.05:0.1:80;

[0023] (2) Soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; acetic acid, citric acid, and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; deionized water is heated to 50°C, a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 2000 rpm for 30 minutes, the mixture is cooled to 25°C, an intermediate A, a neutral cellulase, and a bromelain are added, and the mixture is stirred at 50 rpm for 40 minutes to obtain a highly effective bactericidal and mild laundry detergent; the mass ratio of the deionized water, the surfactant, the auxiliary agent, 4-formylphenylboric acid, the intermediate A, the neutral cellulase, and the bromelain is 50:20:4:0.5:1:1:0.5.

[0024] Example 2

[0025] (1) A 4.7 wt% aqueous solution of polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, lipase, and alkaline protease were mixed, stirred at 80 rpm for 30 min, a 2 wt% aqueous solution of β-cyclodextrin was added, and stirring was continued for 12 h, followed by centrifugation at a speed of 12000 rpm for 25 min, the supernatant was discarded, and the remaining part was washed with deionized water for 3 times, and freeze-dried at a temperature of -30°C for 24 h to obtain an intermediate A; the mass ratio of the 4.7 wt% aqueous solution of the triblock copolymer, lipase, alkaline protease, and 2 wt% aqueous solution of β-cyclodextrin was 10:0.075:0.1:90;

[0026] (2) Soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; acetic acid, citric acid, and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; deionized water is heated to 65°C, a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 3000rpm for 50min, the mixture is cooled to 30°C, an intermediate A, a neutral cellulase, and a bromelain are added, and the mixture is stirred at 75rpm for 65min to obtain a highly effective bactericidal and mild laundry detergent; the mass ratio of the deionized water, the surfactant, the auxiliary agent, 4-formylphenylboric acid, the intermediate A, the neutral cellulase, and the bromelain is 80:35:6:2:1:1:0.5.

[0027] Example 3

[0028] (1) A 4.7 wt% aqueous solution of polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, lipase, and alkaline protease were mixed, stirred at 100 rpm for 40 min, a 2 wt% aqueous solution of β-cyclodextrin was added, and stirring was continued for 15 h, followed by centrifugation at a speed of 15,000 rpm for 40 min, the supernatant was discarded, and the remaining part was washed with deionized water for 5 times, and freeze-dried at a temperature of -35°C for 30 h to obtain an intermediate A; the mass ratio of the 4.7 wt% aqueous solution of the triblock copolymer, lipase, alkaline protease, and 2 wt% aqueous solution of β-cyclodextrin was 10:0.1:0.1:100;

[0029] (2) Soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; acetic acid, citric acid, and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; deionized water is heated to 75°C, a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 4000 rpm for 80 min, the mixture is cooled to 40°C, an intermediate A, a neutral cellulase, and a bromelain are added, and the mixture is stirred at 100 rpm for 90 min to obtain a highly effective bactericidal and mild laundry detergent; the mass ratio of the deionized water, the surfactant, the auxiliary agent, 4-formylphenylboric acid, the intermediate A, the neutral cellulase, and the bromelain is 100:50:8:5:1:1:0.5.

[0030] Comparative Example 1

[0031] The difference between Comparative Example 1 and Example 2 is that step (1) and step (2) are different. Step (1) is changed to: 4.7wt% polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer aqueous solution and alkaline protease are mixed, stirred at 80rpm for 30min, 2wt% β-cyclodextrin aqueous solution is added, stirring is continued for 12h, and then centrifuged at a speed of 12000rpm for 25min, the supernatant is poured out, and the remaining part is washed with deionized water for 3 times, and freeze-dried at a temperature of -30°C for 24h to obtain intermediate A; the mass ratio of the 4.7wt% triblock copolymer aqueous solution, alkaline protease, and 2wt% β-cyclodextrin aqueous solution is 10:0.1:90;

[0032] The step (2) is changed to: soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; acetic acid, citric acid and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; the deionized water is heated to 65°C, the surfactant, the auxiliary agent and 4-formylphenylboric acid are added, and the mixture is stirred at 3000 rpm for 50 min, the mixture is cooled to 30°C, the intermediate A is added, and the mixture is stirred at 75 rpm for 65 min to obtain a highly effective bactericidal mild laundry detergent; the mass ratio of the deionized water, the surfactant, the auxiliary agent, the 4-formylphenylboric acid and the intermediate A is 80:35:6:2:1; and the remaining steps are the same as those in Example 2.

[0033] Comparative Example 2

[0034] The difference between Comparative Example 2 and Example 2 is that there is no step (1), and step (2) is changed to: soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; acetic acid, citric acid, and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; deionized water is heated to 65°C, a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 3000rpm for 50min, cooled to 30°C, intermediate A, neutral cellulase, and bromelain are added, and the mixture is stirred at 75rpm for 65min to obtain a highly efficient sterilizing and mild laundry detergent; the mass ratio of the deionized water, surfactant, auxiliary agent, 4-formylphenylboric acid, alkaline protease, lipase, neutral cellulase, and bromelain is 80:35:6:2:0.5:0.5:1:0.5; and the remaining steps are the same as those in Example 2.

[0035] Comparative Example 3

[0036] The difference between Comparative Example 3 and Example 2 is that step (2) is different. Step (2) is changed to: soybean lecithin and fatty alcohol polyoxyethylene ether are compounded in a mass ratio of 1:1 to obtain a surfactant; sodium citrate and para-chloro-metaxylenol are mixed in a mass ratio of 5:1:0.5 to obtain an auxiliary agent; deionized water is heated to 65°C, a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 3000rpm for 50min, cooled to 30°C, intermediate A, neutral cellulase, and bromelain are added, and the mixture is stirred at 75rpm for 65min to obtain a highly efficient sterilizing and mild laundry detergent; the mass ratio of the deionized water, surfactant, auxiliary agent, 4-formylphenylboric acid, intermediate A, neutral cellulase, and bromelain is 80:35:6:2:1:1:0.5; and the remaining steps are the same as those in Example 2.

[0037] Effect example

[0038] The following Table 1 shows the performance analysis results of the highly effective bactericidal mild laundry detergents of Examples 1 to 3 of the present invention and Comparative Examples 1 to 3.

[0039] Table 1

[0040]

[0041] From the comparison of the experimental data of the embodiment and the comparative example in Table 1, it can be found that the present invention uses neutral cellulase, bromelain, lipase and alkaline protease to prepare a composite enzyme, wherein the neutral cellulase first acts on the non-crystalline area on the surface of the fabric, and under the joint action of its endo- and exo-enzymes, the cavities and capillaries in the fabric fibers are expanded, which is conducive to the stains mixed between the fabric fibers to be more easily contacted with bromelain, lipase and alkaline protease, so that the stubborn stains embedded in the fiber are better removed, and then, citric acid and acetic acid are used to provide a weakly acidic system for the washing environment, which plays a role in softening and caring clothes, thereby achieving mildness, and at the same time, calcium and magnesium ions in hard water will not be deposited, so as not to weaken the washing ability, and the activity of cellulase is improved in a weakly acidic environment, thereby enhancing the decontamination ability of the laundry detergent, wherein the lipase and alkaline protease are encapsulated in a rigid-flexible supramolecular hollow microsphere system, so that the two enzymes are not disturbed in the weakly acidic laundry detergent, and the activity of the alkaline protease is protected, thereby enhancing the decontamination ability of the laundry detergent.

[0042] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations within the meaning and scope of the equivalent elements of the claims be included in the invention. Any marking in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A highly effective bactericidal mild laundry detergent, characterized in that: The high-efficiency bactericidal mild laundry detergent is prepared from complex enzymes, surfactants, enzyme stabilizers and other additives.

2. A highly effective bactericidal mild laundry detergent according to claim 1, characterized in that: The complex enzyme consists of neutral cellulase, bromelain, lipase and alkaline protease.

3. A method for preparing a highly effective bactericidal mild laundry detergent, characterized in that: The method comprises the following preparation steps: (1) A 4.7 wt% triblock copolymer aqueous solution, lipase, and alkaline protease were mixed, stirred at 50-100 rpm for 20-40 min, a 2 wt% β-cyclodextrin aqueous solution was added, and stirring was continued for 10-15 h, and then centrifuged, the supernatant was discarded, and the remaining part was washed with deionized water for 2-5 times, and freeze-dried to obtain intermediate A; (2) The deionized water is heated to 50-75° C., a surfactant, an auxiliary agent, and 4-formylphenylboric acid are added, and the mixture is stirred at 2000-4000 rpm for 30-80 min. The mixture is cooled to 25-40° C., intermediate A, neutral cellulase, and bromelain are added, and the mixture is stirred at 50-100 rpm for 40-90 min to obtain a highly effective bactericidal and mild laundry detergent.

4. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The triblock copolymer in step (1) is specifically a polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer.

5. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The centrifugal speed in step (1) is 10000-15000 rpm and the time is 15-40 min.

6. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The freeze-drying temperature of step (1) is -20 to -35°C and the time is 18 to 30 hours.

7. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The mass ratio of the 4.7wt% triblock copolymer aqueous solution, lipase, alkaline protease, and 2wt% β-cyclodextrin aqueous solution in step (1) is 10:0.05-0.1:0.1:80-100.

8. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The surfactant in step (2) is prepared by compounding soybean lecithin and fatty alcohol polyoxyethylene ether in a mass ratio of 1:

1.

9. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The auxiliary agent in step (2) is prepared by mixing acetic acid, citric acid and para-chloro-meta-xylenol in a mass ratio of 5:1:0.

5.

10. The method for preparing a highly effective bactericidal mild laundry detergent according to claim 3, characterized in that: The mass ratio of the deionized water, surfactant, auxiliary agent, 4-formylphenylboric acid, intermediate A, neutral cellulase and bromelain in step (2) is 50-100:20-50:4-8:0.5-5:1:1:0.5.

Images