Reaction equipment based on vaccine production

Abstract

The invention discloses vaccine production-based reaction equipment, which comprises a culture appliance for culturing cells to reproduce the cells; the opening is formed in the culture device and is used for continuously conveying the culture solution; the two arc-shaped channels are formed in the culture appliance and are combined into a complete annular groove; the liquid conveying pipeline is fixedly connected with the culture device, is communicated with the arc-shaped channel and is arranged opposite to the opening direction; the baffle plate is arranged in a sliding manner relative to the culture device, is positioned in the middle of the two arc-shaped channels and separates the two arc-shaped channels; and the two inclined parts are rotationally connected with the baffle and are arranged on the two sides of the baffle correspondingly. The culture solution conveyed by the peristaltic pump is shunted in two directions, and the power of the two shunted culture solutions rushes to the partition plates to open the partition plates and impact each other, so that kinetic energy is counteracted, the flow rate of the culture solution is reduced, and the flow rate of the culture solution in dynamic cell culture is controlled.

Description

Technical Field

[0001] The invention relates to the technical field of tissue cell culture, in particular to a vaccine production reaction device. Background Art

[0002] Vaccine production equipment contains instruments for cell culture. Specifically, culture dishes are used to culture cells on a small scale, which is the initial step in vaccine production. Rotary bottle culture systems are then used, which are large-scale cell culture devices. Cells are evenly distributed on the bottle walls to obtain sufficient oxygen and nutrients.

[0003] Cell culture requires nutrient supply, which can be divided into static culture and dynamic culture according to the different ways of nutrient solution supply. Static culture is to pre-place a certain amount of nutrient solution and cells in the culture room. Since the capacity of the cell culture medium is fixed, as the culture time increases, the nutrients gradually decrease, and the waste produced by metabolism will gradually accumulate, thus affecting the normal growth of the cells. Dynamic culture means that the nutrient solution can be continuously supplied, fresh culture solution is continuously supplied to the cells, and metabolic products are recovered. Dynamic culture can ensure that the cells always get sufficient fresh nutrient solution throughout the cell culture process, which is more suitable for long-term cell culture.

[0004] In the dynamic cell culture process, the requirements of each stage are different. For example, in the early stage of culture, cells need to adhere to the wall for development and growth. At this moment, the flow rate of the culture medium needs to be slowed down and controlled. In the middle and late stages of culture, cells are fully developed and require large amounts of nutrients, which are less affected by the flow rate of the culture medium. The culture medium is delivered by a peristaltic pump, and the metabolic products are taken away by the peristaltic pump. The constant power of the peristaltic pump causes the flow rate of the culture medium to be relatively stable, which is not suitable for the early stages of dynamic cell culture. Summary of the invention

[0005] The purpose of the present invention is to provide a vaccine production reaction device for controlling the flow rate of culture fluid in dynamic cell culture.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A vaccine production reaction device, comprising:

[0008] Culture equipment, used to culture cells for their reproduction;

[0009] An opening is provided on the culture vessel for continuous delivery of the culture solution;

[0010] Two arc-shaped channels are formed inside the culture vessel and merge into a complete annular groove;

[0011] The infusion pipeline is fixedly connected to the culture apparatus and communicated with the arc-shaped channel, and is arranged opposite to the opening direction;

[0012] The baffle is slidably arranged with the culture apparatus, is located in the middle of the two arc-shaped channels and separates the two arc-shaped channels;

[0013] Two inclined parts are rotatably connected to the baffle and are respectively arranged on both sides of the baffle;

[0014] The culture fluid enters the culture device through the infusion pipe, flows through two arc-shaped channels toward the baffle, and impacts the inclined portion, causing the baffle to slide under the force of the inclined direction, and the two flows impact each other at the baffle.

[0015] Preferably, the culture vessel comprises:

[0016] Chassis;

[0017] The outer ring is fixedly connected to the chassis coaxially;

[0018] At least one culture ring is fixedly connected to the base plate coaxially and located inside the outer ring;

[0019] A top plate, the seal is arranged just above the outer ring and the culture ring;

[0020] The excretion duct is fixedly connected to the top plate and is used to discharge cellular metabolic products.

[0021] Preferably, a sliding seal is arranged between the baffle and the outer ring, an adjustment seat is fixedly connected to the outer edge of the outer ring, a first slide plate and a second slide plate are slidingly arranged inside the adjustment seat, the first slide plate is fixedly connected to one side of the baffle, the second slide plate is fixedly connected to a threaded rod, a plurality of springs are arranged between the first slide plate and the second slide plate, a threaded hole is provided on the adjustment seat, and the threaded hole and the threaded rod are threadedly connected.

[0022] Preferably, the inclined portion comprises:

[0023] Tilt plate;

[0024] The spherical body is movably connected to one side of the inclined plate by ropes;

[0025] The outer ring is provided with a limiting groove, the spherical body slides inside the limiting groove, and the other side of the inclined plate is rotatably connected with the baffle.

[0026] Preferably, the outer ring seal is slidingly provided with a sliding rod, and rubber sleeves are fixedly sleeved at both ends of the sliding rod to prevent the sliding rod from falling off the outer ring. A rotating plate is rotatably connected to the inner ring of the outer ring, and the height of the rotating plate is adapted to the distance between the top plate and the bottom plate. When one of the rubber sleeves contacts one side of the rotating plate, the sliding of the sliding rod can control the rotation angle of the rotating plate in the outer ring.

[0027] Preferably, the interior of the sliding rod is filled with magnetic blocks having two magnetic poles, N and S, which correspond to the directions of the two ends of the sliding rod respectively. Connecting rings are fixedly sleeved on the upper and lower outer edges of the outer ring. Ring grooves are provided on the upper and lower connecting rings. An arc-shaped magnetic plate is slidably arranged between the upper and lower connecting rings. The arc-shaped magnetic plate is adapted to the ring grooves and is slidably limitedly connected to the ring grooves. The arc-shaped magnetic plate has two magnetic poles, N and S. One side of the magnetic plate is connected to the first slide plate through a wire that passes through the adjustment seat.

[0028] Preferably, a plurality of vortex grooves are provided on a side of the rotating plate facing the flow of the culture solution, and the vortex grooves are arranged in this manner by a plurality of transverse grooves and a plurality of winding grooves.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention divides the culture fluid delivered by the peristaltic pump into two directions, and the power of the two divided culture fluids rushes to the partition to open the partition and impact each other, thereby offsetting the kinetic energy and reducing the flow rate of the culture fluid, so as to control the flow rate of the culture fluid in dynamic cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of the structure of a vaccine production reaction device proposed by the present invention;

[0032] Figure 2 This is a schematic diagram of the internal structure of a vaccine production reaction device proposed by the present invention;

[0033] Figure 3 This is a schematic diagram of the internal structure of an adjustment seat in a vaccine production reaction device proposed by the present invention;

[0034] Figure 4 This is a schematic diagram of a top view of the structure of a vaccine production reaction device proposed by the present invention after the top plate is removed;

[0035] Figure 5 This is a schematic diagram of the structure of a rotating plate and a baffle on an outer ring in a vaccine production reaction device proposed by the present invention;

[0036] Figure 6 This is a schematic diagram of the structure of a sliding rod on an outer ring in a vaccine production reaction device proposed by the present invention;

[0037] Figure 7 This is a schematic diagram of the structure of an arc-shaped magnetic plate in a vaccine production reaction device proposed by the present invention.

[0038] In the figure: 1. opening; 2. arc-shaped channel; 3. infusion pipeline; 4. baffle; 5. bottom plate; 6. outer ring; 7. culture ring; 8. top plate; 9. excretion pipeline; 10. adjustment seat; 11. first slide plate; 12. second slide plate; 13. threaded rod; 14. threaded hole; 15. inclined plate; 16. spherical body; 17. limit groove; 18. slide rod; 19. rubber sleeve; 20. magnetic block; 21. connecting ring; 22. ring groove; 23. arc-shaped magnetic plate; 24. wire; 25. vortex groove; 26. spring; 27. rotating plate. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0040] Reference Figure 1 -Attached Figure 7 , a vaccine production reaction device, comprising:

[0041] Culture equipment, used to culture cells for their reproduction;

[0042] An opening 1 is provided on the culture apparatus for continuous delivery of the culture solution;

[0043] Two arc-shaped channels 2 are formed inside the culture vessel and merge into a complete annular groove;

[0044] The infusion pipeline 3 is fixedly connected to the culture device and communicated with the arc-shaped channel 2, and is arranged opposite to the opening 1;

[0045] The baffle 4 is slidably arranged with the culture apparatus, is located in the middle of the two arc-shaped channels 2 and separates the two arc-shaped channels 2;

[0046] Two inclined parts are rotatably connected to the baffle 4 and are respectively arranged on both sides of the baffle 4;

[0047] The culture fluid enters the culture device through the infusion pipe 3, flows through the two arc-shaped channels 2 and flows toward the baffle 4, impacting the inclined portion so that the baffle 4 slides under the force of the inclined direction, and the two flows impact each other at the baffle 4.

[0048] Through the above technical solution, the culture solution transported by the peristaltic pump is split into two directions, and the power of the two split culture solutions rushes to the partition to open the partition and impact each other, thereby offsetting the kinetic energy and reducing the flow rate of the culture solution.

[0049] The culture equipment includes:

[0050] Chassis 5;

[0051] The outer ring 6 is fixedly connected to the chassis 5 coaxially;

[0052] At least one culture ring 7, fixedly connected to the base plate 5 coaxially and located inside the outer ring 6;

[0053] A top plate 8, which is sealed and arranged just above the outer ring 6 and the culture ring 7;

[0054] The drainage pipe 9 is fixedly connected to the top plate 8 and is used for discharging cell metabolic products.

[0055] A sliding seal is arranged between the baffle 4 and the outer ring 6, an adjusting seat 10 is fixedly connected to the outer edge of the outer ring 6, a first slide plate 11 and a second slide plate 12 are slidingly arranged inside the adjusting seat 10, the first slide plate 11 is fixedly connected to one side of the baffle 4, the second slide plate 12 is fixedly connected to a threaded rod 13, a plurality of springs 26 are arranged between the first slide plate 11 and the second slide plate 12, a threaded hole 14 is provided on the adjusting seat 10, and the threaded hole 14 and the threaded rod 13 are threadedly connected.

[0056] Through the above technical scheme, the elastic pressure of the spring 26 on the first slide 11 can be changed by adjusting the threaded rod 13. If it is in the early stage of cell culture, the adjustment of the threaded rod 13 weakens the elastic pressure of the spring 26 on the first slide 11, and the power of the two culture fluids rushes to the partition to open the partition to a greater extent, so that the two culture fluids have a larger counter-attack area, more kinetic energy is offset, and the flow rate of the culture fluid after entering the culture ring 7 through the opening 1 is smaller. If it is in the middle and late stages of cell culture, the adjustment of the threaded rod 13 strengthens the elastic pressure of the spring 26 on the first slide 11, reduces the counter-attack area of ​​the two culture fluids, reduces kinetic energy offset, and enables the culture fluid to flow smoothly, thereby meeting the demand for nutrients in the middle and late stages of cell culture.

[0057] The inclined portion comprises:

[0058] Inclined plate 15;

[0059] The spherical body 16 is movably connected to one side of the inclined plate 15 through a rope;

[0060] The outer ring 6 is provided with a limiting groove 17 , the spherical body 16 is located inside the limiting groove 17 and slides, and the other side of the inclined plate 15 is rotationally connected to the baffle 4 .

[0061] The outer ring 6 is provided with a sliding rod 18 for sealing sliding. Both ends of the sliding rod 18 are fixedly sleeved with rubber sleeves 19 to prevent the sliding rod 18 from falling off the outer ring 6. The inner ring of the outer ring 6 is rotatably connected with a rotating plate 27. The height of the rotating plate 27 is adapted to the distance between the top plate 8 and the bottom plate 5. If one of the rubber sleeves 19 contacts one side of the rotating plate 27, the sliding of the sliding rod 18 can control the rotation angle of the rotating plate 27 in the outer ring 6.

[0062] Through the above technical solution, the sliding of the slide rod 18 can control the rotation angle of the rotating plate 27 in the outer ring 6, and then control the size of the diameter of the arc channel 2. If the diameter is reduced, the culture fluid therein is obstructed and the flow rate is reduced, otherwise the flow rate increases.

[0063] The interior of the slide bar 18 is filled with a magnetic block 20 having two magnetic poles, N and S, corresponding to the directions of the two ends of the slide bar 18 respectively. Connecting rings 21 are fixedly sleeved on the upper and lower outer edges of the outer ring 6. An annular groove 22 is provided on the upper and lower connecting rings 21. An arc-shaped magnetic plate 23 is slidably arranged between the upper and lower connecting rings 21. The arc-shaped magnetic plate 23 is adapted to the annular groove 22 and is slidably limitedly connected to the annular groove 22. The arc-shaped magnetic plate 23 has two magnetic poles, N and S. One side of the magnetic plate is connected to the first slide plate 11 through a wire 24 that passes through the adjustment seat 10.

[0064] Through the above technical solution, the sliding of the first slide plate 11 under the force of the baffle 4 will inevitably pull the wire 24, thereby pulling the arc magnetic plate 23, so that the arc magnetic plate 23 and the slide rod 18 repel each other, so that the slide rod 18 extends into the outer ring 6 as much as possible, pushing the rotating plate 27 to rotate, thereby reducing the diameter of the arc channel 2, and the culture fluid therein is hindered, resulting in a decrease in flow rate, and vice versa.

[0065] A plurality of vortex grooves 25 are formed on one side of the rotating plate 27 facing the flow of the culture solution. The vortex grooves 25 are arranged in this manner by a plurality of transverse grooves and a plurality of winding grooves.

[0066] Through the above technical solution, when the flowing culture fluid rushes to the side of the rotating plate 27 with the vortex groove 25, it is guided by the negative space in the vortex groove 25 to form multiple scrolling flows, which are mixed in the culture fluid to form the offset of the flow in different directions. The culture fluid in it is obstructed and the flow rate is reduced.

[0067] Working principle:

[0068] In the early stage of cell culture, the threaded rod 13 is adjusted to move away from the opening 1, and the culture fluid is transported by the peristaltic pump through the infusion pipe 3 into the culture device, and is divided at the two arc-shaped channels 2 and flows toward the baffle 4, impacting the inclined portion, causing the baffle 4 to slide away from the opening 1, thereby squeezing the spring 26 and pulling the wire 24;

[0069] The wire 24 is conducted to the arc-shaped magnetic plate 23 to make it slide and move. According to the principle of mutual repulsion of like poles, the slide bar 18 pushes the rotating plate 27, reducing the diameter of the arc-shaped channel 2, slowing down the flow rate of the culture fluid, and after the flow rate slows down, the baffle 4 and the rotating plate 27 will return to their original state;

[0070] In the middle and late stages of cell culture, the threaded rod 13 is adjusted to move toward the opening 1, and the culture fluid is transported by the peristaltic pump through the infusion pipe 3 into the culture vessel, where it is divided at the two arc-shaped channels 2 and flows toward the baffle 4, impacting the inclined portion. Since the elastic pressure of the spring 26 on the baffle 4 is relatively large, the impact force of the culture fluid is relatively small, and the baffle 4 directly enters the opening 1.

[0071] It should be noted that:

[0072] The sealed connection and sealing arrangement described above all adopt a sealing ring structure to keep the sliding structure and the fixed structure sealed to prevent the culture fluid from contacting the outside world. This is a prior art and will not be described in detail here.

[0073] A counterweight may be placed on the top plate 8 to cover and press the top plate 8 to stabilize it on the outer ring 6 and the culture ring 7;

[0074] Only one culture ring 7 is shown in the schematic diagram, but more culture rings 7 can be provided as needed, and the culture rings 7 can be provided with diameters decreasing one by one;

[0075] The spherical body 16 is located inside the limiting groove 17 and can slide without falling out;

[0076] The cells are cultured on the inner wall of the culture ring 7 .

[0077] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A vaccine production reaction device, characterized in that: include: Culture equipment, used to culture cells for their reproduction; An opening (1) is provided on the culture device for continuous delivery of culture solution; Two arc-shaped channels (2) are formed inside the culture vessel and merge into a complete annular groove; A liquid infusion pipeline (3) is fixedly connected to the culture device and communicated with the arc-shaped channel (2), and is arranged opposite to the opening (1); A baffle (4) is slidably arranged with the culture device and is located in the middle of the two arc-shaped channels (2) and separates the two arc-shaped channels (2); Two inclined portions are rotatably connected to the baffle plate (4) and are respectively arranged on both sides of the baffle plate (4); The culture fluid enters the culture device through the infusion pipe (3), flows through the two arc-shaped channels (2) and flows toward the baffle (4), impacting the inclined portion so that the baffle (4) slides due to the force in the inclined direction, and the two flows impact each other at the baffle (4).

2. A vaccine production reaction device according to claim 1, characterized in that: The culture equipment includes: Chassis (5); The outer ring (6) is fixedly connected to the chassis (5) coaxially; At least one culture ring (7) is coaxially fixedly connected to the base plate (5) and is located inside the outer ring (6); A top plate (8) is sealingly arranged just above the outer ring (6) and the culture ring (7); The excretion pipe (9) is fixedly connected to the top plate (8) and is used to discharge cell metabolic products.

3. A vaccine production reaction device according to claim 2, characterized in that: A sliding seal is arranged between the baffle (4) and the outer ring (6); an adjustment seat (10) is fixedly connected to the outer edge of the outer ring (6); a first slide plate (11) and a second slide plate (12) are slidably arranged inside the adjustment seat (10); the first slide plate (11) is fixedly connected to one side of the baffle (4); the second slide plate (12) is fixedly connected to a threaded rod (13); a plurality of springs (26) are arranged between the first slide plate (11) and the second slide plate (12); a threaded hole (14) is provided on the adjustment seat (10); and the threaded hole (14) and the threaded rod (13) are threadedly connected.

4. A vaccine production reaction device according to claim 3, characterized in that: The inclined portion comprises: Inclined plate (15); The spherical body (16) is movably connected to one side of the inclined plate (15) via a rope; The outer ring (6) is provided with a limiting groove (17), the spherical body (16) is located inside the limiting groove (17) and slides, and the other side of the inclined plate (15) is rotationally connected to the baffle (4).

5. A vaccine production reaction device according to claim 3, characterized in that: The outer ring (6) is provided with a sliding rod (18) for sealing and sliding. Both ends of the sliding rod (18) are fixedly sleeved with rubber sleeves (19) to prevent the sliding rod (18) from falling off the outer ring (6). The inner ring of the outer ring (6) is rotatably connected with a rotating plate (27). The height of the rotating plate (27) is adapted to the distance between the top plate (8) and the bottom plate (5). When one of the rubber sleeves (19) contacts one side of the rotating plate (27), the sliding of the sliding rod (18) can control the rotation angle of the rotating plate (27) in the outer ring (6).

6. A vaccine production reaction device according to claim 5, characterized in that: The interior of the slide bar (18) is filled with a magnetic block (20) having two magnetic poles, N and S, which correspond to the directions of the two ends of the slide bar (18) respectively. A connecting ring (21) is fixedly sleeved on the upper and lower outer edges of the outer ring (6). Both the upper and lower connecting rings (21) are provided with an annular groove (22). An arc-shaped magnetic plate (23) is slidably arranged between the upper and lower connecting rings (21). The arc-shaped magnetic plate (23) is adapted to the annular groove (22) and is slidably limitedly connected to the annular groove (22). The arc-shaped magnetic plate (23) has two magnetic poles, N and S. One side of the magnetic plate is connected to the first slide plate (11) through a wire (24) that passes through the adjustment seat (10).

7. A vaccine production reaction device according to claim 5, characterized in that: A plurality of vortex grooves (25) are provided on the side of the rotating plate (27) facing the flow of the culture fluid, and the vortex grooves (25) are arranged in this manner by a plurality of transverse grooves and a plurality of winding grooves.

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