Thermal control system for cell culture device

Abstract

The invention discloses a heat control system for a cell culture device, which comprises a heating unit, a centrifugal culture device and the heating unit are arranged in a culture chamber which can be opened or closed, and the heating unit supplies wind power through a first fan to blow hot air into the culture chamber so as to heat the centrifugal culture device; the refrigeration unit is used for discharging heat generated in the refrigeration process through wind power provided by a second fan; the first draught fan and the second draught fan are arranged in the culture room, the communicating air channel is connected to the culture room and the second draught fan, one side of the communicating air channel is connected with a communicating hinge capable of being opened and closed in a hinged mode, wind power of the second draught fan is introduced into the culture room through the communicating air channel, and the wind power of the first draught fan and the wind power of the second draught fan can act on the communicating hinge to open the communicating hinge. According to the invention, hot air blown by the second fan can be introduced into the culture chamber, so that the heating process is quicker, the early heating time is shortened, the working power of the heating unit can be reduced, and the energy consumption is effectively reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a heat control system for a cell culture device. Background Art

[0002] Large-scale expansion of stem cells in vitro is a key step in stem cell research and application, and is of great significance for promoting the development of stem cell therapy, regenerative medicine and other fields. In order to ensure efficient cell expansion and long-term storage, the cell culture environment needs to be heated and maintained at around 37°C to increase cell activity, while the cell culture materials and collected mature cells need to be refrigerated to achieve low-temperature refrigeration, such as maintaining the environment below 8°C. In existing cell culture devices, the heating system and the refrigeration system work independently, both of which consume high power, and it takes a long time to prepare to reach the required working temperature. Summary of the invention

[0003] In order to overcome the deficiencies of the prior art, an object of the present invention is to provide a heat control system for a cell culture device, which has the advantages of effectively reducing energy consumption and shortening the pre-preparation time.

[0004] The purpose of the present invention is achieved by the following technical solutions: According to an embodiment of the present disclosure, a heat control system for a cell culture device is provided, comprising: A heating unit for heating the centrifugal culture device to a predetermined temperature range, wherein the centrifugal culture device and the heating unit are placed in an openable or closed culture chamber, and the heating unit provides wind force through a first fan to blow hot air into the culture chamber to heat the centrifugal culture device; A refrigeration unit for low-temperature refrigeration of cell samples, culture materials and product bags, wherein the refrigeration unit uses a second fan to provide wind power to discharge heat generated during the refrigeration process; and A connecting air duct is connected to the culture chamber and the second fan, and a connecting page that can be opened and closed is hingedly connected to one side of the connecting air duct connected to the culture chamber. The wind force of the second fan is introduced into the culture chamber through the connecting air duct, and the wind force of the first fan and the second fan can act on the connecting page to open the connecting page.

[0005] To implement the above technical solution, when cell culture is carried out, the heating unit starts the first fan to blow hot air into the culture chamber to heat the centrifugal culture device to a predetermined temperature range, so that the cells are at an optimal culture temperature. At the same time, the refrigeration unit starts to refrigerate the cell samples, culture materials and product bags, and the heat generated during the refrigeration process is taken away by the wind force of the second fan, and the hot air brought out by the second fan is introduced into the culture chamber through the connecting air duct. The wind force of the first fan and the second fan can push the connecting page to open, so that the hot air blown by the second fan can smoothly enter the culture chamber, so that the heating process is faster, the initial heating time is shortened, and the working power of the heating unit can be reduced, thereby effectively reducing energy consumption.

[0006] In some exemplary embodiments, the culture chamber includes: a sealed box, and a sealed cover hinged to the sealed box, and a communication port is opened on one side of the sealed box for communication with the communication air duct.

[0007] In some exemplary embodiments, the heating unit comprises: A partition chamber is provided in the culture chamber, wherein the bottom of the partition chamber is provided with an air inlet and the top of the partition chamber is provided with an air outlet: A heating wire disposed in the partition compartment and located near the air outlet; and A first fan is disposed in the partition compartment, wherein an air inlet side of the first fan is close to the air inlet, and an air outlet side of the first fan is close to the heating wire.

[0008] To implement the above technical solution, during heating, the heating wire and the first fan are started, and hot air is blown into the culture chamber by the first fan, so that the centrifugal culture chamber is in an environment within a predetermined temperature range.

[0009] In some exemplary embodiments, the communication port is communicated with the partition compartment and is located above the first fan.

[0010] By implementing the above technical solution, the hot air blown by the second fan can be blown into the culture room by the wind force of the first fan, further improving the heating efficiency.

[0011] In some exemplary embodiments, the refrigeration unit comprises: Refrigeration compartment for storing reagent bags or product bags; A semiconductor refrigerator fitted with the refrigeration bin, wherein the cold end of the semiconductor refrigerator fits with the refrigeration bin; A heat sink fitted with the hot end of the semiconductor refrigerator; and The second fan connected to the radiator is used for cooling the radiator, and the connecting air duct is connected to the second fan.

[0012] To implement the above technical solution, when the semiconductor refrigerator is started, its cold end cools the refrigeration bin, so that the refrigeration bin is in a low-temperature environment, thereby achieving low-temperature refrigeration of the reagent bags and product bags, while its hot end is in contact with the radiator, heat is conducted out through the radiator, and the radiator is dissipated by the second fan, thereby achieving rapid cooling of the radiator.

[0013] In some exemplary embodiments, the refrigeration compartment includes: a refrigeration box, and a refrigeration cover hinged to the refrigeration box.

[0014] In some exemplary embodiments, the bottom wall of the cold storage box is inclined from both sides to the middle to form a water collecting groove in the middle of the cold storage box, and the water collecting groove is connected to a condensation pipe.

[0015] To implement the above technical solution, since condensed water is generated during the refrigeration process, the condensed water will flow along the inclined bottom wall of the cold storage box to the water collecting tank and finally be discharged from the condenser pipe, thereby quickly discharging the condensed water, reducing bacterial growth and preventing the accumulation of condensed water from affecting the refrigeration effect.

[0016] In some exemplary embodiments, a wind shield is provided at the bottom of the radiator, the second fan is fixed to the wind shield, and a vent corresponding to the second fan is provided on the wind shield.

[0017] In some exemplary embodiments, a first temperature sensor is provided in the culture chamber for collecting temperature information in the culture chamber, and a second temperature sensor is provided in the refrigeration warehouse for collecting temperature information in the refrigeration warehouse.

[0018] By implementing the above technical solution and collecting temperature information through the first temperature sensor and the second temperature sensor, the working power of the heating wire and the semiconductor refrigerator can be adjusted in time.

[0019] In some exemplary embodiments, the first fan is a cross-flow fan, and the second fan is a cooling fan.

[0020] In summary, compared with the prior art, the present invention has the following beneficial effects: The embodiment of the present invention provides a heat control system for a cell culture device, comprising: a heating unit for heating a centrifugal culture device to a predetermined temperature range, wherein the centrifugal culture device and the heating unit are placed in an openable or closed culture chamber, and the heating unit provides wind force through a first fan to blow hot air into the culture chamber to heat the centrifugal culture device; a refrigeration unit for performing low-temperature refrigeration treatment on cell samples, culture materials and product bags, and the refrigeration unit provides wind force through a second fan to discharge heat generated during the refrigeration process; and a connecting air duct connected to the culture chamber and the second fan, wherein the connecting air duct is hingedly connected to a side of the culture chamber with an openable and closable connecting page, the wind force of the second fan is introduced into the culture chamber by the connecting air duct, and the wind force of the first fan and the second fan can act on the connecting page to open the connecting page. When conducting cell culture, the heating unit starts to blow hot air from the first fan to the culture chamber to heat the centrifugal culture device to a predetermined temperature range, thereby keeping the cells at an optimal culture temperature. At the same time, the refrigeration unit starts to refrigerate the cell samples, culture materials and product bags, and the heat generated during the refrigeration process is taken away by the wind of the second fan, and the hot air brought out by the second fan is introduced into the culture chamber through the connecting air duct. The wind force of the first fan and the second fan can push the connecting page to open, so that the hot air blown by the second fan can smoothly enter the culture chamber, thereby making the heating process faster, shortening the initial heating time, and reducing the working power of the heating unit, thereby effectively reducing energy consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Schematic diagram of the structure of a heat control system for a cell culture device according to an embodiment of the present invention.

[0022] Figure 2 It is a schematic cross-sectional structure diagram of a heat control system for a cell culture device according to an embodiment of the present invention.

[0023] Figure 3 Schematic diagram of the structure of a refrigeration unit in an embodiment of the present invention.

[0024] The numbers and letters in the figure represent the corresponding component names: 10. Heating unit; 11. Partition compartment; 12. Air inlet; 13. Air outlet; 14. Heating wire; 15. First fan; 16. First temperature sensor; 20. Refrigeration unit; 21. Refrigeration compartment; 211. Refrigeration box; 212. Refrigeration cover; 213. Water collecting tank; 214. Condenser; 22. Semiconductor refrigerator; 23. Radiator; 24. Second fan; 25. Wind shield; 26. Second temperature sensor; 30. Connecting air duct; 31. Connecting page; 32. Evaporation channel; 40. Cultivation chamber; 41. Sealing box; 42. Sealing cover; 43. Connecting port; 50. Centrifugal cultivation device. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] like Figures 1 to 3 As shown, an embodiment of the present invention provides a heat control system for a cell culture device, comprising: a heating unit 10 for heating a centrifugal culture device 50 to a predetermined temperature range, wherein the centrifugal culture device 50 and the heating unit 10 are placed in an openable or closed culture chamber 40, and the heating unit 10 provides wind force through a first fan 15 to blow hot air into the culture chamber 40 to heat the centrifugal culture device 50; a refrigeration unit 20 for performing low-temperature refrigeration treatment on cell samples, culture materials and product bags, and the refrigeration unit 20 provides wind force through a second fan 24 to discharge heat generated during the refrigeration process; and a connecting air duct 30 connected to the culture chamber 40 and the second fan 24, wherein a connecting air duct 30 is hingedly connected to a side of the culture chamber 40 with an openable and closable connecting page 31, and the wind force of the second fan 24 is introduced into the culture chamber 40 through the connecting air duct 30, and the wind force of the first fan 15 and the second fan 24 can act on the connecting page 31 to open the connecting page 31.

[0027] Specifically, the culture chamber 40 includes: a sealing box 41 fixed to the frame, and a sealing cover 42 hinged to the sealing box 41. The sealing cover 42 can be set to be transparent so as to observe the working status of the centrifugal culture device 50. The sealing cover 42 is hingedly connected to the sealing box 41 through a hinge, and a rubber sealing belt is embedded on the top of the sealing box 41. When the sealing cover 42 is closed, it is tightly matched with the rubber sealing belt to form a sealed space in the culture chamber 40 to reduce heat loss. At the same time, in order to avoid heat loss in the culture chamber 40 as much as possible, the sealing box 41 and the sealing cover 42 are made of materials with low thermal conductivity. For example, the material of the sealing cover 42 is selected from heat-insulating resin and the material of the sealing box 41 is selected from engineering plastics. It can be understood that a wire hole should also be set on the side of the culture chamber 40 for the passage of pipelines and control lines.

[0028] A heating unit 10: a partition chamber 11 disposed in the culture chamber 40, the partition chamber 11 having an air inlet 12 at the bottom and an air outlet 13 at the top; a heating wire 14 disposed in the partition chamber 11 and located near the air outlet 13; and a first fan 15 disposed in the partition chamber 11, the air inlet side of the first fan 15 being close to the air inlet 12 and the air outlet side being close to the heating wire 14, and the first fan 15 being a cross-flow fan.

[0029] Among them, the partition compartment 11 is fixed to one side of the culture chamber 40, the air inlet 12 and the air outlet 13 can be set to a rectangular long groove shape or a plurality of through-hole shapes, the heating wire 14 is used to generate heat, and the material of the heating wire 14 can be, for example, iron-chromium-aluminum alloy or nickel-chromium alloy, which has the advantages of high heat transfer efficiency and no harmful substances. A first temperature sensor 16 is provided in the culture chamber 40 for real-time detection and feedback of temperature information in the culture chamber 40. One or more first temperature sensors 16 can be provided. For example, a first temperature sensor 16 can be provided near the air outlet 13 to detect the outlet air temperature. After maintaining the outlet air temperature within a predetermined temperature range for a certain period of time, the culture chamber 40 can reach a suitable temperature. The first temperature sensor 16 can specifically adopt an NTC thermistor temperature sensor.

[0030] The centrifugal culture chamber is thermally insulated by the culture chamber 40. When heating, the heating wire 14 and the first fan 15 are started, and hot air is blown into the culture chamber 40 by the first fan 15, and a circulating air convection environment is formed in the culture chamber 40, so that the temperature in the culture chamber 40 is evenly heated, so that the centrifugal culture chamber is in an environment within a predetermined temperature range. At the same time, the temperature information in the culture chamber 40 is detected in real time by the temperature sensor, so that the working power of the heating wire 14 can be adjusted in time.

[0031] At the same time, a connecting port 43 is opened on one side of the sealing box 41 for connecting with the connecting air duct 30. The connecting port 43 is connected to the partition chamber 11 and is located above the first fan 15, so that the hot air blown by the second fan 24 can be blown into the culture chamber 40 by the wind force of the first fan 15, thereby further improving the heating efficiency. The connecting page 31 is set at the connecting port 43 and is used to control the opening or closing of the connecting port 43.

[0032] The refrigeration unit 20 includes: a refrigeration bin 21 for storing reagent bags or product bags; a semiconductor refrigerator 22 fitted with the refrigeration bin 21, and the cold end of the semiconductor refrigerator 22 fits with the refrigeration bin 21; a radiator 23 fitted with the hot end of the semiconductor refrigerator 22; and a second fan 24 connected to the radiator 23, for dissipating heat from the radiator 23, the connecting air duct 30 is connected to the second fan 24, and the second fan 24 uses a heat dissipation fan.

[0033] The refrigeration chamber 21 includes: a refrigeration box 211, and a refrigeration cover 212 hinged to the refrigeration box 211, the refrigeration cover 212 is hingedly connected to the refrigeration box 211 through a hinge, and a heat preservation cotton layer can be arranged in the refrigeration chamber 21 to improve the heat preservation performance of the refrigeration chamber 21, so that the refrigeration chamber 21 can maintain a low temperature state for a long time, the semiconductor refrigerator 22 specifically adopts a semiconductor Peltier, and the semiconductor refrigerator 22 is arranged at the bottom of the refrigeration chamber 21, and the radiator 23 includes a substrate and a plurality of heat dissipation devices evenly arranged on the substrate. The fins and the base plate are fitted with the semiconductor refrigerator 22, and a wind shield 25 is also provided at the bottom of the radiator 23. The wind shield 25 is connected and fixed to the radiator 23 through an L-shaped connecting piece arranged on the side of the radiator 23. The wind shield 25 is used to provide an installation basis for the cooling fan and can form a wind gathering effect. The second fan 24 is fixed to the bottom of the wind shield 25. One or more second fans 24 can be provided, and ventilation holes are provided on the wind shield 25 corresponding to each second fan 24 for the wind of the second fan 24 to pass through.

[0034] When the semiconductor refrigerator 22 is started, its cold end cools the refrigeration bin 21, so that the refrigeration bin 21 is in a low-temperature environment, thereby realizing low-temperature refrigeration of reagent bags and product bags, while its hot end is in contact with the radiator 23, and heat is conducted out through the radiator 23, and the radiator 23 is heat-dissipated by the second fan 24, thereby realizing rapid cooling of the radiator 23; in some embodiments, one or more second temperature sensors 26 may also be provided in the refrigeration bin 21, and the temperature in the refrigeration bin 21 is detected by the second temperature sensor 26, so as to control the semiconductor refrigerator 22 to adjust the working power in time and keep the refrigeration bin 21 in a suitable low-temperature environment.

[0035] The bottom wall of the cold storage box 211 is inclined from the middle of the two sides to form a water collecting groove 213 in the middle of the cold storage box 211, and the water collecting groove 213 is connected to the condenser 214. The condenser 214 can extend downward and be connected to a waste liquid collection box. Since condensed water will be generated during the refrigeration process, the condensed water will flow along the inclined bottom wall of the cold storage box 211 to the water collecting groove 213, and finally be discharged from the condenser 214, so that the condensed water can be quickly discharged, reducing bacterial growth and avoiding the accumulation of condensed water affecting the refrigeration effect.

[0036] In this embodiment, the condenser 214 is made of capillary fibers, which can absorb condensed water more efficiently through capillary action, and can also have a larger surface area when outside the cold storage box 211 to evaporate the condensed water more quickly, thereby reducing the space for collecting waste liquid; at the same time, an evaporation channel 32 is provided on the connecting air duct 30, and the condenser 214 penetrates into the evaporation channel 32 to facilitate the hot air discharged by the second fan 24 to evaporate the condensed water, thereby further improving the evaporation efficiency and reducing the heat dissipated by the entire system.

[0037] When cell culture is performed, the heating unit 10 starts to blow hot air from the first fan 15 to the culture chamber 40 to heat the centrifugal culture device 50 to a predetermined temperature range, so that the cells are at an optimal culture temperature. At the same time, the refrigeration unit 20 starts to refrigerate the cell samples, culture materials and product bags, and the heat generated during the refrigeration process is taken away by the wind of the second fan 24. The hot air brought out by the second fan 24 is introduced into the culture chamber 40 through the connecting air duct 30. The wind power of the first fan 15 and the second fan 24 can push the connecting page 31 to open, so that The hot air blown by the second fan 24 can smoothly enter the culture chamber 40, thereby making the heating process faster, shortening the initial heating time, and reducing the working power of the heating unit 10, thereby effectively reducing energy consumption. At the same time, by adjusting the air volume of the first fan 15 and the second fan 24, the opening range of the connecting page 31 can be changed, thereby adjusting the connection amount with the culture bin, that is, changing the air outlet at the connecting port 43. When the connection amount is large, the airflow can be supplemented through the connecting port 43. When the connection amount becomes small, the connecting port 43 can be used as an exhaust port.

[0038] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which are equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of them belong to the protection scope of the present invention.

Claims

1. A heat control system for a cell culture device, characterized in that: include: A heating unit for heating the centrifugal culture device to a predetermined temperature range, wherein the centrifugal culture device and the heating unit are placed in an openable or closed culture chamber, and the heating unit provides wind force through a first fan to blow hot air into the culture chamber to heat the centrifugal culture device; A refrigeration unit for low-temperature refrigeration of cell samples, culture materials and product bags, wherein the refrigeration unit uses a second fan to provide wind power to discharge heat generated during the refrigeration process; and A connecting air duct is connected to the culture chamber and the second fan, and a connecting page that can be opened and closed is hingedly connected to one side of the connecting air duct connected to the culture chamber. The wind force of the second fan is introduced into the culture chamber through the connecting air duct, and the wind force of the first fan and the second fan can act on the connecting page to open the connecting page.

2. The heat control system for a cell culture device according to claim 1, characterized in that: The culture chamber comprises: a sealing box and a sealing cover hinged to the sealing box. A communication port is provided on one side of the sealing box for communicating with the communication air duct.

3. The heat control system for a cell culture device according to claim 2, characterized in that: The heating unit comprises: A partition chamber is provided in the culture chamber, wherein the bottom of the partition chamber is provided with an air inlet and the top of the partition chamber is provided with an air outlet: A heating wire disposed in the partition compartment and located near the air outlet; and A first fan is disposed in the partition compartment, wherein an air inlet side of the first fan is close to the air inlet, and an air outlet side of the first fan is close to the heating wire.

4. The heat control system for a cell culture device according to claim 3, characterized in that: The communication port is communicated with the partition compartment and is located above the first fan.

5. The heat control system for a cell culture device according to claim 1, characterized in that: The refrigeration unit comprises: Refrigeration compartment for storing reagent bags or product bags; A semiconductor refrigerator fitted with the refrigeration bin, wherein the cold end of the semiconductor refrigerator fits with the refrigeration bin; A heat sink fitted with the hot end of the semiconductor refrigerator; and The second fan connected to the radiator is used for cooling the radiator, and the connecting air duct is connected to the second fan.

6. The heat control system for a cell culture device according to claim 5, characterized in that: The refrigeration warehouse comprises: a refrigeration box and a refrigeration cover hinged to the refrigeration box.

7. The heat control system for a cell culture device according to claim 6, characterized in that: The bottom wall of the refrigerating box is inclined from both sides to the middle to form a water collecting groove in the middle of the refrigerating box, and the water collecting groove is connected to a condensing pipe.

8. The heat control system for a cell culture device according to claim 5, 6 or 7, characterized in that: A wind shield is provided at the bottom of the radiator, the second fan is fixed to the wind shield, and a vent corresponding to the second fan is provided on the wind shield.

9. The heat control system for a cell culture device according to claim 5, characterized in that: The culture chamber is provided with a first temperature sensor for collecting temperature information in the culture chamber, and the refrigeration chamber is provided with a second temperature sensor for collecting temperature information in the refrigeration chamber.

10. The heat control system for a cell culture device according to claim 1, characterized in that: The first fan is a cross-flow fan, and the second fan is a cooling fan.

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