Centrifugal culture bin and centrifugal culture device

Abstract

The invention discloses a centrifugal culture bin and a centrifugal culture device.The centrifugal culture bin comprises an upper bin body and a lower bin body which are matched in a sealed mode, a containing space is formed between the upper bin body and the lower bin body, a liquid inlet assembly is arranged on the lower bin body, and a liquid outlet assembly is arranged on the lower bin body; the rotary sealing body is arranged on the upper bin body; the fixed joint seat is rotationally assembled on the rotary sealing body, a gas-liquid channel and a liquid outlet channel are arranged on the fixed joint seat, and the liquid outlet channel is communicated with the liquid outlet assembly; the blade shaft is rotationally assembled on the rotary sealing body and connected with the fixed connector base, the bottom of the blade shaft is connected with the lower bin body, a through flow guide channel is formed in the blade shaft, and the two ends of the flow guide channel are communicated with the gas-liquid channel and the liquid inlet assembly respectively. According to the centrifugal culture bin, large-scale amplification of cells can be achieved, the cell damage rate is low, and the centrifugal culture bin is simple in overall structure and low in cost.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture devices, and in particular to a centrifugal culture chamber and a centrifugal culture device. Background Art

[0002] The large-scale expansion of stem cells in vitro is a key step in stem cell research and application, and is of great significance for promoting the development of stem cell therapy, regenerative medicine and other fields. The expansion of stem cells in vitro is mainly achieved by isolating, purifying and culturing stem cells to increase their number under sterile conditions. In this process, it is necessary to optimize the conditions such as culture medium components, growth factors and extracellular matrix to promote the proliferation and differentiation of stem cells. Traditional 2D culture technology is simple and it is relatively easy to replace the culture medium, but it is difficult to expand the culture of cells, and it is impossible to monitor the cell growth status and cell growth environment in a timely and effective manner. The 3D culture method can select a large-capacity bioreactor to achieve large-scale cell expansion, and can also use three-dimensional porous structures such as microcarriers, which are conducive to cell adsorption and proliferation, and most of the growth factors are attached to the surface, which is conducive to cell expression, thereby improving the quality and efficiency of stem cell expansion.

[0003] During the culture process, stem cells need to go through repeated separation of intermediate products, fluid replacement and re-culture to ensure the ideal expansion efficiency at a specific stage. Therefore, the culture chamber needs to be able to achieve dynamic 3D culture and also facilitate cell harvesting or fluid replacement operations. Two separation and harvesting schemes, centrifugation and filtration, are commonly used in existing equipment. The centrifugation scheme centrifuges the cultured samples, and after the cell samples and supernatant are separated, the supernatant is removed, the cells are washed, and the cells are concentrated; the filtration harvesting scheme filters the cell samples containing the supernatant through the filter, which can filter out the supernatant while intercepting the cells. However, the flow resistance of the filter is large, resulting in low separation efficiency, and long-term treatment will also significantly reduce the biological activity of the cells; therefore, the centrifugation scheme has a higher throughput and can meet the needs of large-volume cell sample solution separation. However, the existing centrifugal chamber structure process is relatively complex and the production cost is high, which makes it difficult to meet the growing demand for stem cell culture. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the object of the present invention is to provide a centrifugal culture chamber and a centrifugal culture device, which have the advantages of simple structure, low cost, and easy to achieve large-scale cell preparation.

[0005] The purpose of the present invention is achieved by the following technical solutions: According to a first aspect of an embodiment of the present disclosure, there is provided a centrifugal culture chamber, comprising: An upper bin body and a lower bin body sealed and matched with each other, a containing space is formed between the upper bin body and the lower bin body, and a liquid inlet component is provided on the lower bin body, and a liquid outlet component is provided on the lower bin body; A rotating sealing body disposed on the upper storage body; A fixed joint seat rotatably mounted on the rotary seal body, wherein the fixed joint seat is provided with a gas-liquid channel and a liquid outlet channel, wherein the liquid outlet channel is connected to the liquid outlet assembly; and A blade shaft is rotatably assembled on the rotating seal body and connected to the fixed joint seat, the bottom of the blade shaft is connected to the lower warehouse body, and a through guide channel is provided on the blade shaft, the first end of the guide channel is connected to the gas-liquid channel, and the second end is connected to the liquid inlet component.

[0006] To implement the above technical solution, the fixed joint seat is used to connect the gas control system and the liquid control system. The gas-liquid channel allows mixed gas, cell samples and culture materials to enter the centrifugal culture chamber. When supplied, the mixed gas, cell samples and culture materials are transferred and drained through the guide channel on the blade shaft, and then introduced into the centrifugal culture chamber by the liquid inlet component to realize liquid inlet control, and the liquid outlet channel is connected to the liquid outlet component to discharge the supernatant, etc., to realize liquid replacement operation; because the fixed joint seat is rotatably connected to the rotating sealing body, the connection with the gas control system and the liquid control system will not be affected when the centrifugal culture chamber rotates, and the setting of the blade shaft can provide turbulence during the rotation of the centrifugal culture chamber, promote the convection exchange of nutrients, thereby realizing cell expansion, and the cell damage rate is low, and the overall structure of the centrifugal culture chamber is simple and the cost is low.

[0007] In some exemplary embodiments, the liquid inlet assembly includes: A liquid inlet ring seat, wherein the liquid inlet ring seat is provided with a rotation groove adapted to the bottom of the blade shaft, and the rotation groove is provided with a liquid through port connected to the guide channel; A guide plate connected to the side of the liquid inlet ring seat and extending in the radial direction of the liquid inlet ring seat; The lower bin body is provided with an installation groove adapted to the liquid inlet ring seat and the guide plate, and the installation groove is connected to the liquid through port, and a guide groove is formed between the liquid inlet ring seat and the guide plate, and a liquid inlet connected to the lower bin body is formed at the end of the guide groove.

[0008] To implement the above technical solution, the rotating groove cooperates with the bottom of the blade shaft to achieve a stable connection with the blade shaft. The airflow and liquid flow enter the guide groove from the liquid port after being guided by the guide channel. Restricted by the liquid inlet ring seat and the guide plate, the airflow and liquid flow are finally forced to enter the centrifugal culture chamber from the liquid inlet. At the same time, since the liquid inlet component is arranged in the lower chamber body, the airflow direction and liquid flow direction are from bottom to top during supply, which can promote the dissolution and exchange of gas in the culture solution and ensure the pH and dissolved oxygen level of the solution.

[0009] In some exemplary embodiments, the liquid outlet assembly includes a liquid outlet guide plate fixed to the upper warehouse body, the liquid outlet guide plate extends from the rotating seal body to near the lower warehouse body, the rotating seal body is provided with a transition guide groove connected to the liquid outlet channel, the liquid outlet guide plate is provided with a liquid outlet guide groove connected to the transition guide groove, and the liquid outlet guide groove extends to one end near the lower warehouse body to form a liquid outlet.

[0010] To implement the above technical solution, when discharging liquid, the liquid flows from the liquid outlet into the liquid outlet guide groove, and under the drainage effect of the liquid outlet guide groove, enters the liquid outlet channel through the transfer guide groove to realize liquid discharge.

[0011] In some exemplary embodiments, the upper warehouse body includes an integrally formed top plate, an upper connecting plate and an upper surrounding plate, the upper connecting plate is obliquely arranged between the top plate and the upper surrounding plate to form a bowl-shaped structure, a through installation opening is opened in the middle of the top plate for assembling a rotating seal body, and an interlocking step is formed between the upper connecting plate and the upper surrounding plate for abutting cooperation with the lower warehouse body.

[0012] In some exemplary embodiments, a positioning flange is provided on the outer side of the top plate, and a positioning mounting portion adapted to the positioning flange is provided on the upper end of the rotating seal body. When the rotating seal body is installed in the mounting port, the positioning mounting portion is embedded in the positioning flange.

[0013] In some exemplary embodiments, the lower storage body includes an integrally formed bottom plate, a lower connecting plate and a lower enclosure plate, the lower connecting plate is obliquely disposed between the bottom plate and the lower enclosure plate to form a bowl-shaped structure, the lower enclosure plate and the upper enclosure plate are mutually buckled, and the lower enclosure plate abuts against the fitting step; The end of the lower connecting plate expands radially outward to form an annular belt between the lower connecting plate and the lower enclosure plate. The annular belt is used to form a cell concentration area during centrifugation, and the liquid outlet is located above the radial position of the annular belt.

[0014] In some exemplary embodiments, a first step shaft and a second step shaft are formed on the fixed joint seat, the second step shaft extends downward and is connected to the blade shaft, a first bearing surface and a second bearing surface are formed in the rotary seal body, an upper bearing is provided between the first bearing surface and the first step shaft, and a lower bearing is provided between the second bearing surface and the second step shaft.

[0015] The above technical solution is implemented to achieve the rotational connection between the fixed joint seat and the rotating sealing body.

[0016] In some exemplary embodiments, a positioning shoulder is provided on the outside of the fixed joint seat, and the positioning shoulder abuts against the upper end of the upper bearing to limit the installation position of the fixed joint seat, the liquid outlet channel passes through the lower end surface of the first step shaft, and a flow channel step surface is provided in the middle portion of the rotating seal body, and the lower end surface of the first step shaft and the flow channel step surface form a transition guide groove connected to the liquid outlet channel.

[0017] According to a second aspect of an embodiment of the present disclosure, there is provided a centrifugal culture device, comprising: Centrifugal support frame; The centrifugal culture chamber as described in the first aspect, wherein the centrifugal culture chamber is rotatably mounted on the centrifugal support frame; and A first driving motor is carried by the centrifugal support frame and is used to drive the centrifugal culture chamber to perform centrifugal rotational motion.

[0018] To implement the above technical solution, the containing space formed in the centrifugal culture chamber can be used to contain cells and culture materials. The cells and culture materials can be supplied and discharged through the liquid inlet component and the liquid outlet component, and the centrifugal culture chamber can be driven to rotate by the first drive motor to realize the suspension culture and centrifugal stratification of cells.

[0019] In some exemplary embodiments, a monitoring feedback module is further included, wherein the monitoring feedback module includes: A detection sensor for detecting the pH value and DO value in the centrifugal culture chamber; and The detection camera is used to perform layered monitoring on the centrifugal culture chamber.

[0020] To implement the above technical solution, the pH value, DO value and stratification detection are realized through the monitoring feedback module, so as to regulate the gas circuit control system and the liquid circuit control system.

[0021] In summary, compared with the prior art, the present invention has the following beneficial effects: The embodiment of the present invention provides a centrifugal culture chamber and a centrifugal culture device, wherein a fixed joint seat is used to connect a gas circuit control system and a liquid circuit control system, and a gas-liquid channel is used for mixed gas, cell samples and culture materials to enter the centrifugal culture chamber. When supplied, the mixed gas, cell samples and culture materials are transferred and drained through the guide channel on the blade shaft, and then introduced into the centrifugal culture chamber by the liquid inlet component to realize liquid inlet control, and the liquid outlet channel is connected to the liquid outlet component to discharge supernatant, etc., to realize liquid replacement operation; since the fixed joint seat is rotatably connected to the rotating sealing body, the connection with the gas circuit control system and the liquid circuit control system will not be affected when the centrifugal culture chamber rotates, and the setting of the blade shaft can provide turbulence during the rotation of the centrifugal culture chamber, promote the convection exchange of nutrients, thereby realizing cell proliferation, and the cell damage rate is low, and the overall structure of the centrifugal culture chamber is simple and the cost is low. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the structure of the centrifugal culture chamber in an embodiment of the present invention.

[0023] Figure 2 It is a cross-sectional view of a centrifugal culture chamber in an embodiment of the present invention.

[0024] Figure 3 for Figure 2 Enlarged view of part A in the middle.

[0025] Figure 4 Schematic diagram of the structure of the upper storage body in an embodiment of the present invention.

[0026] Figure 5 This is a schematic structural diagram of the upper storage body from another perspective in an embodiment of the present invention.

[0027] Figure 6 It is a schematic diagram of the half-section structure of the rotary seal body in an embodiment of the present invention.

[0028] Figure 7 It is a schematic diagram of the half-section structure of the fixed joint seat in the embodiment of the present invention.

[0029] Figure 8 Schematic diagram of the structure of the liquid outlet component in an embodiment of the present invention.

[0030] Fig. 9 Schematic diagram of the structure of the lower bin body in an embodiment of the present invention.

[0031] Fig.10 Schematic diagram of the structure of the liquid inlet component in an embodiment of the present invention.

[0032] Fig.11 Schematic diagram of the structure of the centrifugal culture device in an embodiment of the present invention.

[0033] The numbers and letters in the figure represent the corresponding component names: 10. Centrifugal culture device; 11. Centrifugal support frame; 12. Centrifugal culture chamber; 121. Upper chamber body; 1211. Top plate; 1212. Upper connecting plate; 1213. Upper enclosure; 1214. Mounting port; 1215. Positioning flange; 1216. Liquid outlet groove; 1217. Guide hole; 122. Lower chamber body; 1221. Bottom plate; 1222. Lower connecting plate; 1223. Lower enclosure; 1224. Mounting groove; 1225. Guide groove; 1226. Liquid inlet; 1227. Cell concentration area; 123. Rotary seal body; 1231. Transfer guide groove; 1232. Upper retaining ring; 1233. Positioning and mounting portion; 1234. First positioning hole; 1235. First bearing surface; 1236. First Second bearing surface; 1237, flow channel step surface; 124, fixed joint seat; 1241, gas-liquid channel; 1242, liquid outlet channel; 1243, first step shaft; 1244, second step shaft; 1245, upper bearing; 1246, positioning shoulder; 1247, lower bearing; 1248, sealing ring; 125, blade shaft; 1251, guide channel; 1252, turbulent blade; 13, liquid inlet assembly; 131, liquid inlet ring seat; 132, rotating groove; 133, liquid through port; 134, guide plate; 14, liquid outlet assembly; 141, liquid outlet guide plate; 142, liquid outlet guide groove; 143, liquid outlet; 15, first drive motor; 20, monitoring feedback module; 21, detection sensor; 22, detection camera. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] like Figures 1 to 10 As shown, the first aspect of the embodiment of the present invention provides a centrifugal culture chamber 12, comprising: an upper chamber body 121 and a lower chamber body 122 that are sealed and matched with each other, and a storage space is formed between the upper chamber body 121 and the lower chamber body 122; a rotating sealing body 123 arranged on the upper chamber body 121; a fixed joint seat 124 rotatably assembled on the rotating sealing body 123, and a gas-liquid channel 1241 and a liquid outlet channel 1242 are provided on the fixed joint seat 124, and the liquid outlet channel 1242 is connected to the liquid outlet component 14; and a blade shaft 125 rotatably assembled on the rotating sealing body 123 and connected to the fixed joint seat 124, the bottom of the blade shaft 125 is connected to the lower chamber body 122, and a through guide channel 1251 is provided on the blade shaft 125, and the first end of the guide channel 1251 is connected to the gas-liquid channel 1241, and the second end is connected to the liquid inlet component 13.

[0036] Among them, Figures 2 to 7 As shown, the upper storage body 121 includes an integrally formed top plate 1211, an upper connecting plate 1212 and an upper enclosure plate 1213, the upper enclosure plate 1213 and the top plate 1211 are perpendicular to each other, the upper connecting plate 1212 is obliquely arranged between the top plate 1211 and the upper enclosure plate 1213 to form a bowl-shaped structure, the inclination angle of the upper connecting plate 1212 can be set to 40-50 degrees, preferably 45 degrees, a through installation opening 1214 is opened in the middle of the top plate 1211 for assembling the rotating seal body 123, the rotating seal body 123 and the upper storage body 121 are clamped and fixed by an upper retaining spring 1232, specifically: a positioning flange 1215 is arranged on the outer side of the top plate 1211, and the rotating seal body 123 is fixed to the upper storage body 121 by the upper retaining spring 1232. The upper end of the sealing body 123 is provided with a positioning and mounting portion 1233 that is compatible with the positioning flange 1215. When the rotating sealing body 123 is inserted into the mounting port 1214, the positioning and mounting portion 1233 is embedded in the positioning flange 1215, and an upper clamping groove is provided on the rotating sealing body 123. The upper retaining spring 1232 is embedded in the upper clamping groove and abuts against the inner wall of the top plate 1211, and corresponding first positioning holes 1234 are provided on the positioning and mounting portion 1233 and the top plate 1211. The installation position and direction of the rotating sealing body 123 are limited by the first positioning hole 1234; and an interlocking step is formed between the upper connecting plate 1212 and the upper enclosure plate 1213 for abutting and cooperating with the lower warehouse body 122.

[0037] like Figure 2 and Fig. 9 As shown, the lower warehouse body 122 includes an integrally formed bottom plate 1221, a lower connecting plate 1222 and a lower enclosure plate 1223, the lower enclosure plate 1223 and the bottom plate 1221 are perpendicular to each other, the lower connecting plate 1222 is obliquely arranged between the bottom plate 1221 and the lower enclosure plate 1223 to form a bowl-shaped structure, the inclination angle of the lower connecting plate 1222 can be set to 40-50°, for example, preferably to 45°, the outer diameter of the lower enclosure plate 1223 matches the inner diameter of the upper enclosure plate 1213, and the lower enclosure plate 1223 and the upper enclosure plate 1213 are interlocked, and the lower enclosure plate 1223 is in contact with the engaging step.

[0038] like Figure 3 , Figure 6 and Figure 7 As shown, the fixed joint seat 124 and the rotating sealing body 123 are rotationally connected through a bearing, and the top of the gas-liquid channel 1241 is connected with an air circuit interface and a liquid circuit interface, the air circuit interface is used to connect with the air circuit control system, and the liquid circuit interface is used to connect with the liquid circuit control system. The air circuit interface and the liquid circuit interface are respectively used to introduce air flow and liquid flow and converge them to the gas-liquid channel 1241, and the top of the liquid outlet channel 1242 is connected with a liquid outlet interface.

[0039] A first step shaft 1243 and a second step shaft 1244 are formed on the fixed joint seat 124, the second step shaft 1244 extends downward and is connected to the blade shaft 125, a first bearing surface 1235 and a second bearing surface 1236 are formed in the rotary seal body 123, an upper bearing 1245 is provided between the first bearing surface 1235 and the first step shaft 1243, and a positioning shoulder 1246 is provided outside the fixed joint seat 124, the positioning shoulder 1246 is in conflict with the upper end of the upper bearing 1245 to limit the installation position of the fixed joint seat 124, the liquid outlet channel 1242 passes through the lower end surface of the first step shaft 1243, and a flow channel step surface 1237 is provided in the middle of the rotary seal body 123, the lower end surface of the first step shaft 1243 and the flow channel step surface 1237 form a transfer guide groove 1 connected to the liquid outlet channel 1242 231; the second bearing surface 1236 is located below the flow channel step surface 1237, and a lower bearing 1247 is provided between the second bearing surface 1236 and the second step shaft 1244, the gas-liquid channel 1241 passes through the lower end surface of the second step shaft 1244, and is connected to the blade shaft 125 through the second step shaft 1244 to achieve communication with the guide channel 1251; in some embodiments, a sealing ring 1248 is provided between the first step shaft 1243 and the rotating seal body 123, between the second step shaft 1244 and the rotating seal body 123, and between the second step shaft 1244 and the blade shaft 125 to improve the sealing performance, and a plurality of turbulent blades 1252 are also provided in the blade shaft 125 to form turbulence during the rotation of the centrifugal culture chamber 12, and the turbulent blades 1252 are preferably evenly arranged in four groups around the blade shaft 125.

[0040] The fixed joint seat 124 is used to connect the gas control system and the liquid control system. The gas-liquid channel 1241 allows mixed gas, cell samples and culture materials to enter the centrifugal culture chamber 12. When supplied, they are transferred and drained through the guide channel 1251 on the blade shaft 125, and then introduced into the centrifugal culture chamber 12 by the liquid inlet component 13 to achieve liquid inlet control, while the liquid outlet channel 1242 is connected to the liquid outlet component 14, and is used to discharge supernatant, etc. under centrifugal state; since the fixed joint seat 124 is rotatably connected to the rotating sealing body 123, the connection with the gas control system and the liquid control system will not be affected when the centrifugal culture chamber 12 rotates, and the setting of the blade shaft 125 can provide turbulence during the rotation process of the centrifugal culture chamber 12 to promote the convective exchange of nutrients.

[0041] like Fig. 9 and Fig.10As shown, the liquid inlet assembly 13 includes: a liquid inlet ring seat 131, on which is provided a rotating groove 132 which is adapted to the bottom of the blade shaft 125, and in which is provided a liquid through port 133 which is connected to the guide channel 1251; a guide plate 134 which is connected to the side of the liquid inlet ring seat 131 and extends radially along the liquid inlet ring seat 131; and on the lower warehouse body 122, a mounting groove 1224 which is adapted to the liquid inlet ring seat 131 and the guide plate 134 is provided, and the mounting groove 1224 is connected to the liquid through port 133, and a guide groove 1225 is formed between the liquid inlet ring seat 131 and the guide plate 134, and a liquid inlet port 1226 which is connected to the lower warehouse body 122 is formed at the end of the guide groove 1225.

[0042] Specifically, the mounting groove 1224 includes: a central ring groove adapted to the liquid inlet ring seat 131, and a radial groove adapted to the guide plate 134. The liquid inlet component 13 is embedded in the mounting groove 1224 and fits with the mounting groove 1224 to achieve sealing, and can be sealed by ultrasonic welding or photosensitive adhesive bonding. The guide groove 1225 is arranged in the radial groove and extends from the center of the central ring groove to the edge of the bottom plate 1221, and the width of the guide groove 1225 is smaller than the width of the radial groove to limit the embedding depth of the guide plate 134. The guide groove 1225 extends out of the radial groove to form a liquid inlet 1226 at the end.

[0043] The rotating groove 132 cooperates with the bottom of the blade shaft 125 to achieve a stable connection with the blade shaft 125. The airflow and liquid flow are guided through the guide channel 1251 and enter the guide groove 1225 from the liquid port 133. Restricted by the liquid inlet ring seat 131 and the guide plate 134, the airflow and liquid flow are finally forced to enter the centrifugal culture chamber 12 from the liquid inlet 1226. At the same time, since the liquid inlet component 13 is arranged in the lower chamber body 122, the airflow direction and liquid flow direction are from bottom to top during the supply, which can promote the dissolution and exchange of gas in the culture solution and ensure the pH and dissolved oxygen level of the solution.

[0044] like Figures 4 to 8As shown, the liquid outlet assembly 14 includes a liquid outlet guide plate 141 fixed to the upper warehouse body 121, the liquid outlet guide plate 141 extends from the rotating seal body 123 to near the lower warehouse body 122, the rotating seal body 123 is provided with a transfer guide groove 1231 connected to the liquid outlet channel 1242, the liquid outlet guide plate 141 is provided with a liquid outlet guide groove 142 connected to the transfer guide groove 1231, and the liquid outlet guide groove 142 extends to one end near the lower warehouse body 122 to form a liquid outlet 143, the liquid outlet 143 is rectangular, and the liquid outlet 143 is set to be extremely thin, for example, the width of the liquid outlet 143 is set to 0.5mm; it can be understood that a liquid outlet embedded groove 1216 adapted to the liquid outlet guide plate 141 is provided on the upper warehouse body 121, and the liquid outlet guide plate 141 is embedded in the liquid outlet embedded groove 1216 and passes through the ultra The sealing connection is performed by ultrasonic welding or photosensitive adhesive bonding, and a guide hole 1217 is also provided on the upper chamber body 121. The guide hole 1217 is correspondingly connected to the end of the liquid outlet channel 1242. One side of the transfer guide groove 1231 extends in the rotating sealing body 123 to be correspondingly connected to the guide hole 1217. When discharging liquid, the liquid flows into the liquid outlet guide groove 142 from the liquid outlet port 143, and enters the liquid outlet channel 1242 through the transfer guide groove 1231 under the drainage effect of the liquid outlet guide groove 142 to achieve liquid discharge. After the centrifugal culture chamber 12 is centrifuged, the cells are centrifugally attached to the inner wall of the centrifugal culture chamber 12, and a very small concentration space is formed on the inner wall of the centrifugal culture chamber 12, which can meet the high-proportion concentration requirements, and then the supernatant is extracted through the extremely thin liquid outlet port 143, thereby achieving a more accurate and thorough liquid replacement operation.

[0045] It can be understood that the end of the lower connecting plate 1222 expands radially outward to form an annular belt between the lower connecting plate 1222 and the lower enclosure plate 1223. The annular belt can form a cell concentration area 1227 during centrifugation, and the liquid outlet 143 is located above the radial position of the annular belt. In actual use, the radial position of the liquid outlet 143 can be adjusted according to the concentration requirements to ensure that the supernatant can be completely removed.

[0046] In summary, in the centrifugal culture chamber 12 of the present invention, the fixed joint seat 124 is used to connect the gas circuit control system and the liquid circuit control system, and the gas-liquid channel 1241 is used for mixed gas, cell samples and culture materials to enter the centrifugal culture chamber 12. When supplied, the mixed gas, cell samples and culture materials are transferred and drained through the guide channel 1251 on the blade shaft 125, and then introduced into the centrifugal culture chamber 12 by the liquid inlet component 13 to realize liquid inlet control, and the liquid outlet channel 1242 is connected to the liquid outlet component 14 for discharging supernatant, etc.; because the fixed joint seat 124 is rotatably connected to the rotating sealing body 123, the connection with the gas circuit control system and the liquid circuit control system will not be affected when the centrifugal culture chamber 12 rotates, and the setting of the blade shaft 125 can provide turbulence during the rotation process of the centrifugal culture chamber 12, promote the convective exchange of nutrients, and the overall structure of the centrifugal culture chamber 12 is simple and the cost is low.

[0047] like Fig.11 As shown, the second aspect of an embodiment of the present invention provides a cell culture device, comprising: a centrifugal support frame 11; a centrifugal culture chamber 12 as described in the first aspect, the centrifugal culture chamber 12 is rotatably assembled on the centrifugal support frame 11; and a first drive motor 15 carried by the centrifugal support frame 11 and used to drive the centrifugal culture chamber 12 to perform centrifugal rotational motion.

[0048] The centrifugal support frame 11 is used to support and install the centrifugal culture chamber 12. A bearing can be arranged between the bottom of the centrifugal culture chamber 12 and the centrifugal support frame 11 to realize the rotational connection of the centrifugal culture chamber 12. The first drive motor 15 adopts a servo motor, so that it can drive the centrifugal culture chamber 12 to perform intermittent rotation, forward and reverse rotation or high-speed centrifugal rotation; the storage space formed in the centrifugal culture chamber 12 can be used to store cells and culture materials, and the supply and discharge of cells and culture materials are realized through the liquid inlet component 13 and the liquid outlet component 14, and the centrifugal culture chamber 12 can be driven to rotate by the first drive motor 15 to realize the suspension culture and centrifugal stratification of cells.

[0049] Furthermore, the centrifugal culture chamber 12 is provided with a monitoring feedback module 20 on the outside of the centrifugal culture chamber 12 , and the monitoring feedback module 20 includes: a detection sensor 21 for detecting the pH value and DO value in the centrifugal culture chamber 12 ; and a detection camera 22 for performing layered monitoring of the centrifugal culture chamber 12 .

[0050] The detection sensor 21 and the detection camera 22 are tilted at a predetermined angle and direction on a bracket to correspond to the centrifugal culture chamber 12. The detection sensor 21 adopts a non-contact pH and DO fiber optic probe, and a sensor patch is provided on the inner wall of the lower chamber 122. When the detection sensor 21 corresponds to the sensor patch, the pH value and dissolved oxygen level of the current culture medium in the centrifugal culture chamber 12 can be detected after data collection and processing. The collected pH value and DO value can be used as a basis for the gas path control system to adjust the gas concentration. The detection camera 22 can, for example, adopt an industrial CCD camera, which collects image information in the centrifugal culture chamber 12 and compares it with a preset stratified image. When the comparison is consistent, it can be determined that the stratification is completed.

[0051] The pH value, DO value and stratification detection are realized by monitoring the feedback module 20 so as to regulate the gas circuit control system and the liquid circuit control system.

[0052] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which are equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of them belong to the protection scope of the present invention.

Claims

1. A centrifugal culture chamber, characterized in that: include: An upper bin body and a lower bin body sealed and matched with each other, a containing space is formed between the upper bin body and the lower bin body, and a liquid inlet component is provided on the lower bin body, and a liquid outlet component is provided on the lower bin body; A rotating sealing body disposed on the upper storage body; A fixed joint seat rotatably mounted on the rotary seal body, wherein the fixed joint seat is provided with a gas-liquid channel and a liquid outlet channel, wherein the liquid outlet channel is connected to the liquid outlet assembly; and A blade shaft is rotatably assembled on the rotating seal body and connected to the fixed joint seat, the bottom of the blade shaft is connected to the lower warehouse body, and a through guide channel is provided on the blade shaft, the first end of the guide channel is connected to the gas-liquid channel, and the second end is connected to the liquid inlet component.

2. The centrifugal culture chamber according to claim 1, characterized in that: The liquid inlet assembly comprises: A liquid inlet ring seat, wherein the liquid inlet ring seat is provided with a rotation groove adapted to the bottom of the blade shaft, and the rotation groove is provided with a liquid through port connected to the guide channel; A guide plate connected to the side of the liquid inlet ring seat and extending in the radial direction of the liquid inlet ring seat; The lower bin body is provided with an installation groove adapted to the liquid inlet ring seat and the guide plate, and the installation groove is connected to the liquid through port, and a guide groove is formed between the liquid inlet ring seat and the guide plate, and a liquid inlet connected to the lower bin body is formed at the end of the guide groove.

3. The centrifugal culture chamber according to claim 2, characterized in that: The liquid outlet assembly includes a liquid outlet guide plate fixed to the upper bin body, the liquid outlet guide plate extends from the rotating seal body to a position close to the lower bin body, the rotating seal body is provided with a transfer guide groove connected to the liquid outlet channel, the liquid outlet guide plate is provided with a liquid outlet guide groove connected to the transfer guide groove, and the liquid outlet guide groove extends to one end close to the lower bin body to form a liquid outlet.

4. The centrifugal culture chamber according to claim 1, characterized in that: The upper warehouse body includes an integrally formed top plate, an upper connecting plate and an upper enclosure plate. The upper connecting plate is obliquely arranged between the top plate and the upper enclosure plate to form a bowl-shaped structure. A through installation opening is opened in the middle of the top plate for assembling a rotating seal body, and an interlocking step is formed between the upper connecting plate and the upper enclosure plate for abutting cooperation with the lower warehouse body.

5. The centrifugal culture chamber according to claim 4, characterized in that: A positioning flange is arranged on the outer side of the top plate, and a positioning installation portion matched with the positioning flange is arranged on the upper end of the rotary seal body. When the rotary seal body is installed in the installation opening, the positioning installation portion is embedded in the positioning flange.

6. The centrifugal culture chamber according to claim 4 or 5, characterized in that: The lower storage body includes an integrally formed bottom plate, a lower connecting plate and a lower enclosure plate, wherein the lower connecting plate is obliquely arranged between the bottom plate and the lower enclosure plate to form a bowl-shaped structure, and the lower enclosure plate and the upper enclosure plate are mutually buckled, and the lower enclosure plate is abutted against the fitting step; The end of the lower connecting plate expands radially outward to form an annular belt between the lower connecting plate and the lower enclosure plate. The annular belt is used to form a cell concentration area during centrifugation, and the liquid outlet is located above the radial position of the annular belt.

7. The centrifugal culture chamber according to claim 3, characterized in that: A first step shaft and a second step shaft are formed on the fixed joint seat, the second step shaft extends downward and is connected to the blade shaft, a first bearing surface and a second bearing surface are formed in the rotary seal body, an upper bearing is provided between the first bearing surface and the first step shaft, and a lower bearing is provided between the second bearing surface and the second step shaft.

8. The centrifugal culture chamber according to claim 7, characterized in that: A positioning shoulder is provided on the outside of the fixed joint seat, and the positioning shoulder is in conflict with the upper end of the upper bearing to limit the installation position of the fixed joint seat. The liquid outlet channel passes through the lower end surface of the first step shaft, and a flow channel step surface is provided in the middle of the rotating seal body. The lower end surface of the first step shaft and the flow channel step surface form a transition guide groove connected to the liquid outlet channel.

9. A centrifugal culture device, characterized in that: include: Centrifugal support frame; The centrifugal culture chamber according to any one of claims 1 to 8, wherein the centrifugal culture chamber is rotatably mounted on the centrifugal support frame; as well as, A first driving motor is carried by the centrifugal support frame and is used to drive the centrifugal culture chamber to perform centrifugal rotational motion.

10. The centrifugal culture device according to claim 9, characterized in that: It also includes a monitoring feedback module, which includes: A detection sensor for detecting the pH value and DO value in the centrifugal culture chamber; and The detection camera is used to perform layered monitoring on the centrifugal culture chamber.

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