Transcription factor gene osrav9 for regulating drought resistance of rice and application thereof
By cloning and overexpressing the OsRAV9 gene, the problem of insufficient research on drought-resistant genes in rice has been solved, and the drought resistance of rice has been significantly enhanced, providing new genetic resources and theoretical basis for drought-resistant rice breeding.
CN119799777BActive Publication Date: 2026-05-01HUBEI UNIV
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Patent Information
- Application Number
- CN202510159592.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2026-05-01
- Estimated Expiration
- 2045-02-12
AI Technical Summary
Technical Problem
In the current technology, there is insufficient research on drought-resistant genes in rice, which may lead to a decrease or even loss of resistance in drought-resistant varieties after several years of application, affecting rice yield and quality.
Method used
By cloning and overexpressing the OsRAV9 gene, overexpression vectors and CRISPR/Cas9 knockout vectors were constructed to increase the expression level of the OsRAV9 gene in rice and enhance the drought resistance of rice.
Benefits of technology
Overexpression of the OsRAV9 gene significantly improves rice's resistance to drought, while the RAV9 mutant is less drought-resistant, providing new genetic resources and theoretical basis, and laying the foundation for breeding new drought-resistant rice varieties.
✦ Generated by Eureka AI based on patent content.
Abstract
This invention belongs to the field of plant genetic engineering technology, specifically involving the OsRAV9 gene, a transcription factor that regulates drought resistance in rice, and its application. A drought-induced expression of the OsRAV9 gene was screened, its nucleotide sequence being shown in SEQ ID NO:1; the protein sequence encoded by this gene is shown in SEQ ID NO:2. Using Agrobacterium-mediated transformation, OsRAV9 overexpressing transgenic lines and CRISPR / Cas9 mutant lines were obtained. Seedling drought stress experiments showed that under drought stress, the accumulation rate of MDA in OsRAV9 gene-deficient plants was accelerated, and sufficient free proline could not be formed. In contrast, OsRAV9 overexpressing plants showed a significant increase in free proline content and a relatively slower accumulation rate of malondialdehyde (MDA) under drought stress, preliminarily indicating that OsRAV9 positively regulates drought resistance in rice seedlings.
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