A detection method and a sample analyzer

Abstract

This invention discloses a detection method and a sample analyzer. The method includes: controlling a light source device to emit incident light, the incident light at least partially including target incident light; acquiring a detection signal of a first target emitted light and acquiring a detection signal of a second target emitted light; determining a sample detection result based on the detection signals of the first and second target emitted lights; wherein the light intensity value of the target incident light is N times the light intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light whose corresponding emitted light detection signal after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1. Using this invention can improve the accuracy of the measurement results.

Description

Technical Field

[0001] This invention relates to the field of sample detection technology, and in particular to a detection method and a sample analyzer. Background Technology

[0002] Optical measurement systems are commonly used in sample analyzers. Their measurement principle is as follows: a light source (e.g., a light-emitting diode, LED) emits light of a specific wavelength as incident light. After the incident light passes through a reaction vessel containing the sample and reagents, the outgoing light is collected by a back-end detector (e.g., a photodetector, PD) to obtain the light intensity signal. The coagulation reaction result is obtained by detecting the change in absorbance during the sample coagulation reaction within the reaction vessel.

[0003] Sample analyzer measurement systems based on optical measurement methods suffer from inaccurate results due to high concentrations of interfering substances in the sample. Therefore, there is an urgent need to research a detection method to improve the accuracy of measurement results from the optical system of the sample analyzer. Summary of the Invention

[0004] This invention provides a detection method and a sample analyzer that can improve the accuracy of the sample analyzer's measurement results.

[0005] In a first aspect, embodiments of the present invention provide a detection method applied to a sample analyzer, the sample analyzer comprising a processor, a light source device, and a measuring device; the light source device is used to provide incident light of different intensities under the control of the processor; the measuring device is used to detect the detection signal of the emitted light corresponding to the incident light after passing through a reaction vessel containing a mixed solution of sample and reagent; the detection method includes:

[0006] The system controls the light source device to emit incident light, at least partially including target incident light; acquires a detection signal of a first target emitted light, wherein the first target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution in the early stage of the coagulation reaction; acquires a detection signal of a second target emitted light, wherein the second target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution in the later stage of the coagulation reaction; determines the detection result of the sample based on the detection signals of the first and second target emitted light; wherein the light intensity value of the target incident light is N times the light intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light whose detection signal of the emitted light after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0007] In this embodiment, the mixed solution is irradiated with target incident light, i.e., the light source device is adjusted to operate in high-intensity mode. In this mode, the measured signal-to-noise ratio is relatively high. More specifically, since the ratio between the intensity of the target incident light and the intensity of the normal incident light is greater than the ratio between the noise caused by the target incident light and the noise caused by the normal incident light, this ratio is equivalent to the ratio of the change in the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the solidification reaction to the change in the detection signal measured by the measuring device after the normal incident light passes through the mixed solution. Therefore, compared with irradiating the mixed solution with normal incident light, irradiating the mixed solution with target incident light yields a higher signal-to-noise ratio, thereby improving the repeatability and accuracy of the test results.

[0008] In one possible implementation, the value of N is in the range of [5.5, 6.5].

[0009] In this implementation, the intensity of the target incident light is 5.5 to 6.5 times that of the normal incident light. At this intensity, the detection signal obtained by the measuring device directly detecting the target incident light will exceed the measurement range of the measuring device. This direct detection of the target incident light means that the target incident light is detected by the measuring device after passing through air. When high-intensity incident light is used to irradiate the mixed solution, the measured signal-to-noise ratio will be higher, thereby improving the accuracy of the measurement results.

[0010] In one possible implementation, before controlling the light source device to emit incident light, the method further includes: determining the absorbance of the sample solution, wherein the absorbance is greater than a preset threshold.

[0011] In this implementation, when the absorbance of the sample solution exceeds a preset threshold, meaning the sample background is high, the mixed solution is irradiated with target incident light. Because the sample background is high, the detection signal of the first target emitted light will be within the measurement range of the measuring device and will be greater than the detection signal of the emitted light corresponding to the mixed solution irradiated with normal incident light. Furthermore, according to the absorbance calculation formula, when the difference between the absorbance in the early stage of the coagulation reaction (i.e., the baseline period) and the absorbance in the later stage of the coagulation reaction is the same, the higher the detection signal in the early stage of the coagulation reaction, the greater the amplitude of the change in the detection signal during the coagulation reaction. Therefore, when the sample background is too high, controlling the light source to emit target incident light can increase the amplitude of the change in the detection signal measured by the measuring device during the coagulation reaction. Since the ratio of the increase in detection signal is greater than the ratio of the increase in light source noise due to the increase in light intensity, the signal-to-noise ratio increases. This improves the repeatability and accuracy of the test results.

[0012] In one possible implementation, the sample analyzer has an interference detection channel; determining the absorbance of the sample solution includes: controlling the light source device to emit detection incident light, the detection incident light being normal incident light; acquiring a detection signal of the detection emitted light, the detection emitted light being the emitted light corresponding to the detection incident light after passing through the sample solution in the interference detection channel; and determining the absorbance of the sample solution based on the detection signal of the detection emitted light.

[0013] In this implementation, the interference detection channel of the sample analyzer is used to determine the absorbance of the sample solution, and normal incident light is used as the detection incident light. Using normal incident light to detect the sample background ensures accurate measurement regardless of whether the sample background is high or not. This avoids situations where the light source emits target incident light when the sample background is low, causing the detection signal obtained by the measuring device to exceed the measurement range. When the sample background is high, using target incident light improves the accuracy of the test results.

[0014] In one possible implementation, the sample analyzer has a first calibration channel, in which a light intensity attenuator is provided; the method further includes: acquiring the actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; and calibrating the target incident light according to the actual detection signal.

[0015] In this implementation, because the light intensity of the target incident light is large, the detection signal of the corresponding outgoing light exceeds the measurement range of the measuring device, making calibration impossible using conventional methods. Therefore, this embodiment uses a light intensity attenuator to reduce the light intensity of the target incident light during calibration, ensuring that the detection signal of the corresponding outgoing light after passing through the light intensity attenuator is within the measurement range of the second device. The proportion by which the light intensity attenuator reduces the light intensity depends on its transmittance, which is the ratio of the light intensity that can pass through the attenuator to the original light intensity. As an example, the reduction in light intensity is inversely proportional to the transmittance of the attenuator; the lower the transmittance, the greater the reduction in light intensity. As another example, the transmittance of the attenuator is equal to the ratio of the light intensity that the first light signal can pass through the attenuator to the original light intensity of the first light signal.

[0016] In one possible implementation, calibrating the target incident light based on the actual detection signal includes: if the actual detection signal is greater than the target detection signal, controlling the light source device to reduce the operating current of the light source device; if the actual detection signal is less than the target detection signal, controlling the light source device to increase the operating current of the light source device.

[0017] In this implementation, the target incident light is calibrated based on the actual detection signal, which means adjusting the operating current of the light source device. The larger the operating current, the greater the light intensity of the target incident light emitted by the light source device, and vice versa. The actual detection signal is equal to the target detection signal.

[0018] In a second aspect, embodiments of the present invention provide a sample analyzer, comprising: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; a light source device for providing incident light with different light intensities; a measuring device for detecting a detection signal of the emitted light generated after the incident light passes through the reaction cup containing the mixed solution; and a processor connected to the light source device and the measuring device for executing some or all of the method steps described in the first aspect of this embodiment.

[0019] Thirdly, embodiments of the present invention provide a detection method applied to a sample analyzer, the sample analyzer including a processor, a light source device, and a measuring device; the light source device is used to provide incident light of different intensity values ​​under the control of the processor; the detection device is used to detect the detection signal of the corresponding emitted light after the incident light passes through a reaction vessel containing a mixed solution of sample and reagent; the detection method includes:

[0020] The absorbance of the sample solution is determined; based on the absorbance, a target incident light for irradiating the mixed solution of the sample and reagent is determined, wherein the target incident light is one of a first incident light and a second incident light, the intensity of the first incident light being higher than that of the second incident light; the light source device is controlled to emit incident light, the incident light at least partially including the target incident light; a detection signal of a first emitted light is acquired, the first emitted light being the emitted light corresponding to the mixed solution in the early stage of the coagulation reaction after the target incident light irradiates it; a detection signal of a second emitted light is acquired, the second emitted light being the emitted light corresponding to the mixed solution in the later stage of the coagulation reaction after the target incident light irradiates it; and the detection result of the sample solution is determined based on the detection signals of the first and second emitted light.

[0021] In this embodiment, the target incident light is determined based on the absorbance of the sample solution. The target incident light is one of the first incident light and the second incident light, which makes the light intensity value of the incident light irradiating the mixed solution more flexible and can improve the accuracy of the measurement results.

[0022] In one possible implementation, the light intensity of the first incident light is N times the light intensity of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the emitted light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0023] In this implementation, the intensity of the first incident light is N times the intensity of the second incident light, where N is a positive real number greater than 5. In this case, the detection signal of the emitted light after the first incident light passes through air exceeds the measurement range of the measuring device. When the target incident light is the first incident light, the ratio between the intensity of the target incident light and the intensity of the second incident light is greater than the ratio between the noise caused by the target incident light and the noise caused by the second incident light. This ratio is equal to the ratio of the change in the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the solidification reaction to the change in the detection signal measured by the measuring device after the second incident light passes through the mixed solution. Therefore, the signal-to-noise ratio increases, thereby improving the repeatability and accuracy of the test results.

[0024] In one possible implementation, determining the target incident light for irradiating the mixed solution of sample and reagent based on the absorbance includes: if the absorbance is greater than a preset threshold, then determining the target incident light as a first incident light; if the absorbance is less than the preset threshold, then determining the target incident light as a second incident light.

[0025] In this implementation, when the sample absorbance exceeds a preset threshold, the target incident light is designated as the first incident light. Therefore, the detection signal measured by the measuring device is larger in the early stages of the coagulation reaction. Furthermore, according to the absorbance calculation formula, when the difference between the absorbance in the early and later stages of the coagulation reaction is the same, a higher detection signal in the early stages of the coagulation reaction results in a greater amplitude of signal change during the coagulation process. Therefore, when the sample background is too high, designating the target incident light as the first incident light can increase the amplitude of signal change measured by the measuring device during the coagulation reaction. Since the increase in detection signal is greater than the increase in light intensity leading to an increase in light source noise, the signal-to-noise ratio increases. This improves the accuracy of the test results.

[0026] In one possible implementation, the sample analyzer has a first calibration channel, in which a light intensity attenuator is provided; the method further includes: when the target incident light is determined to be the first incident light, acquiring the actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; and calibrating the target incident light according to the actual detection signal.

[0027] For an explanation of this implementation method, please refer to the explanation of the first aspect above, which will not be elaborated here.

[0028] In one possible implementation, the sample analyzer has a second calibration channel; the method further includes: when the target incident light is determined to be the second incident light, acquiring the actual detection signal of the outgoing light corresponding to the target incident light after passing through the second calibration channel; and calibrating the target incident light according to the actual detection signal.

[0029] In this implementation, the second calibration channel is a calibration channel for the second incident light. When the second incident light is used as the target incident light, since the detection signal of the corresponding outgoing light after the second incident light passes through the air is within the measurement range of the measuring device, the second incident light can be directly calibrated according to the actual detection signal.

[0030] In one possible implementation, the sample analyzer has an interference detection channel; determining the absorbance of the sample solution includes: controlling the light source device to emit detection incident light, the detection incident light being normal incident light; acquiring a detection signal of the detection emitted light, the detection emitted light being the emitted light corresponding to the detection incident light after passing through the sample solution in the interference detection channel; and determining the absorbance of the sample solution based on the detection signal of the detection emitted light.

[0031] For an explanation of this implementation method, please refer to the explanation of the first aspect above, which will not be elaborated here.

[0032] In one possible implementation, the detected incident light is normal incident light, wherein the normal incident light is the incident light whose detection signal of the corresponding outgoing light after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0033] In one possible implementation, the sample analyzer includes multiple detection channels; the light source device is used to switch different wavelengths of the incident light during the detection process under the control of the processor; the method further includes: determining a time segment of the target incident light in the incident light according to the detection items of the detection channels and the target incident light; and determining the detection signals of the first and second outgoing light according to the time segment.

[0034] In this implementation, the sample analyzer includes multiple detection channels and can perform multiple detection items simultaneously. During the detection process, the light source device can switch between different wavelengths of the incident light. That is, the incident light emitted by the light source device is a series of light with different wavelengths and light intensities. Each of these series of light occupies a time segment, which includes the target incident light.

[0035] In one possible implementation, after determining that the target incident light is the second incident light, and after acquiring the detection signal of the first emitted light and the detection signal of the second emitted light, the method further includes: amplifying the detection signal of the first emitted light and the detection signal of the second emitted light.

[0036] In this implementation, when the target incident light is the second incident light, the measuring device can be controlled to amplify the detection signals of the first and second outgoing light, which can also increase the signal-to-noise ratio, thereby improving the repeatability and accuracy of the test results.

[0037] Fourthly, embodiments of the present invention provide a sample analyzer, comprising: at least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of a sample and a reagent; a light source device for providing incident light with different light intensities; a measuring device for detecting a detection signal of the emitted light generated after the incident light passes through the reaction cup containing the mixed solution; and a processor connected to the light source device and the measuring device for executing some or all of the method steps described in the third aspect of this embodiment.

[0038] Fifthly, embodiments of this application provide a computer-readable storage medium storing instructions that, when executed on a computer, cause some or all of the method steps described in the first or third aspect of this embodiment to be performed. Attached Figure Description

[0039] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments of the present invention will be described below.

[0040] Figure 1 This is a schematic diagram of the structure of an optical measurement system provided in an embodiment of this application;

[0041] Figure 2 This is a graph showing the absorbance of a common interfering substance provided in the embodiments of this application;

[0042] Figure 3 This is a solidification reaction curve provided in an embodiment of this application;

[0043] Figure 4 This is yet another solidification reaction curve provided in the embodiments of this application;

[0044] Figure 5 This is a schematic flowchart of a detection method provided in an embodiment of this application;

[0045] Figure 6 This is a flowchart illustrating another detection method provided in an embodiment of this application;

[0046] Figure 7 This is a flowchart illustrating another detection method provided in an embodiment of this application;

[0047] Figure 8 This is a schematic diagram of a sample analyzer provided in an embodiment of this application. Detailed Implementation

[0048] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0049] The terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products, or apparatuses.

[0050] In this application, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this application can be combined with other embodiments.

[0051] The technical solution provided in this application can be applied to an optical measurement system, which is a commonly used measurement system in sample analyzers, including coagulation analyzers. Its measurement principle is as follows: light of a specific wavelength emitted by a light source (e.g., an LED) is used as the incident light. After the incident light passes through a reaction container containing a sample or reagent, the emitted light is collected by a downstream measuring device. The coagulation reaction result of the sample is obtained by detecting the change in absorbance during the sample coagulation reaction within the reaction container.

[0052] Please see Figure 1 , Figure 1 This is a schematic diagram of the structure of an optical measurement system provided in an embodiment of this application. Figure 1 As shown, the optical measurement system includes a light source device 101, a reaction cup 102, and a measuring device 103. The measuring device 103 is used to convert light signals into electrical signals. For example, the measuring device 103 can be a photodiode (PD). The light source device 101 emits light signals of different intensities by adjusting its operating current; the light source device 101 can be an LED, and other examples are not listed here. The reaction cup 102 contains samples with different absorbances; this reaction cup is a light-transmitting container, such as a beaker or reaction vessel. The measuring device 103 is used to collect the light signals and convert them into analog electrical signals using a photoelectric sensor, and then convert the analog electrical signals into digital electrical signals. During the solidification reaction, the amplitude of the change in this digital electrical signal is the useful signal obtained through measurement. The light source device 101 can emit light signals of different intensities. Figure 1 The light signal emitted by the light source is the incident light 104, and the light signal after the incident light 104 passes through the reaction cup is the outgoing light 105.

[0053] like Figure 1 As shown, incident light 104 is emitted from the light source, passes through the reaction cup 102, and is measured by the measuring device 103. During the coagulation reaction of the sample in the reaction cup, the absorbance of the sample changes (for example, the absorbance gradually increases during the coagulation reaction), thus the intensity of the emitted light 105 gradually decreases. The measuring device 103 records the change in voltage value obtained by measuring the emitted light 105 during the reaction, which is equivalent to the change in light intensity of the light signal after passing through the reaction cup. It should be noted that during the reaction of the sample, the intensity of the incident light 104 emitted by the light source device 101 is constant.

[0054] Please see Figure 2 , Figure 2 This is a graph showing the absorbance of a common interfering substance as provided in an embodiment of this application. For example... Figure 2 As shown, common interfering agents such as hemolysis, icterus, and lipemia (collectively referred to as HIL interfering agents) exhibit different absorption peaks at various main measurement wavelengths, such as 405nm, 575nm, 660nm, and 800nm, in the optical measurement device of coagulation analyzers. Coagulation analyzers based on optical measurement methods are prone to deviations in test results due to the presence of interfering agents in plasma samples (this refers to measurement-related deviations, excluding pathological deviations). Figure 2 The horizontal axis represents the wavelength of the light signal, and the vertical axis represents the absorbance.

[0055] Please see Figure 3 , Figure 3 This is a solidification reaction curve provided in an embodiment of this application. For example... Figure 3 As shown, the curve represents the test results of a normal plasma sample. The vertical axis represents the voltage value of the sample obtained by the measuring device during the coagulation reaction process. Optionally, the vertical axis represents the digital quantity (Analog-to-digital, AD) output by the measuring device after converting the optical signal into an analog electrical signal and then passing it through an analog-to-digital converter. Figure 3 The variation range of the curve represents the variation range of the voltage value during the solidification reaction process, and the horizontal axis represents the solidification reaction time of the sample.

[0056] like Figure 3 As shown, the coagulation reaction time is started from the moment the last reagent is added to the sample solution and the mixed solution is reinserted into the measurement channel. The voltage value corresponding to the highest point of the curve is the voltage value measured by the measuring device when the incident light passes directly through the air. When the mixed solution is reinserted into the measurement channel, the voltage value drops sharply. Subsequently, the curve enters the first plateau period (i.e., the baseline period or the pre-coagulation reaction period). The higher voltage value obtained by the measuring device from the light signal passing through the sample during the baseline period is because the background of normal plasma samples is low; that is, the absorbance of normal plasma samples is low in the pre-coagulation reaction period, resulting in a higher light intensity value after the light signal passes through the sample. Then, during the coagulation reaction, as the sample absorbance gradually increases, the voltage value obtained by the measuring device from the light signal passing through the sample gradually decreases. Figure 3 During the solidification reaction, the curve gradually decreases. Finally, in the second plateau phase of the curve (i.e., the later stage of the solidification reaction), the absorbance of the sample reaches its maximum value and tends to stabilize (in some cases, it may also rise slowly or fluctuate). Therefore, the voltage value obtained by the measuring device from the light signal after passing through the sample reaches its minimum value and tends to stabilize (in some cases, it may also decrease slowly).

[0057] Please see Figure 4 , Figure 4 This is yet another solidification reaction curve provided in the embodiments of this application. For example... Figure 4 As shown, the curve represents the test results for a lipid-rich sample. Figure 4 The x and y coordinates are referenced above. Figure 3 The explanation of the horizontal and vertical axes is not detailed here. Figure 4 As shown, the coagulation reaction curve of the lipemia sample exhibits a relatively small change in voltage value during the coagulation reaction, much smaller than that of the standard coagulation reaction. Figure 3The voltage change in normal plasma samples during the coagulation reaction is as follows: Compared to normal plasma samples, lipemic samples have a higher concentration of interfering substances, meaning their absorbance (background) is higher. For samples with a high background, the high absorbance in the early stages of the coagulation reaction leads to a lower voltage value measured by the measuring device during this phase. Furthermore, because the absorbance change is smaller throughout the entire coagulation reaction, the voltage change measured by the measuring device is also smaller. In other words, the useful signal affecting the measurement results is smaller, resulting in a lower signal-to-noise ratio and consequently, poor repeatability and accuracy. Figure 4 For details on the solidification reaction curve, please refer to the above text. Figure 3 The explanation will not be elaborated here.

[0058] To address the aforementioned problems, this application provides a measurement method that can resolve the issue of inaccurate measurement results. The method provided in this application will be described in detail below.

[0059] Please see Figure 5 , Figure 5 This is a schematic flowchart of a detection method provided in an embodiment of this application. For a description of the light source device and measuring device involved in the embodiments of this application, please refer to the above description. Figure 1 The details of the above process will not be elaborated here. The following will provide a detailed explanation of the steps involved in the above process.

[0060] 501. Control the light source device to emit incident light, wherein at least a portion of the incident light includes target incident light.

[0061] Wherein, the light intensity value of the target incident light is N times the light intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light whose detection signal of the corresponding outgoing light after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1. Optionally, the value range of N is [5.5, 6.5].

[0062] For example, the measuring device may employ a 12-bit, 16-bit, or 24-bit digital-to-analog converter chip; in some examples, their measurement range may be [-2.5V, +2.5V]. When the maximum value of the normal light intensity is directly measured, it can reach 70%-80% of the full voltage of the measuring device, preferably 75% of the full voltage of the chip. The light intensity value of the target incident light (i.e., the high light intensity value) can be between 5.7 and 6.3 times the normal light intensity value (at its maximum value), preferably 5.9 times. In the embodiments of this application, the detection signal obtained by directly detecting the target incident light by the measuring device will exceed the measurement range of the measuring device. When the mixed solution is irradiated with high-intensity incident light, the measured signal-to-noise ratio will be larger, thereby improving the accuracy of the measurement results.

[0063] For example, the incident light emitted by the light source device is a series of lights with different wavelengths and intensities. For instance, the first incident light has a wavelength of 405 nm and an intensity of 26,000, the second incident light has a wavelength of 405 nm and an intensity of 154,000, the third incident light has a wavelength of 660 nm and an intensity of 26,000, the fourth incident light has a wavelength of 660 nm and an intensity of 154,000, and so on. These will not be listed exhaustively here. The aforementioned target incident light can be a portion of the series of lights emitted by the light source device. Correspondingly, at the measuring device end, the measuring device detects the target outgoing light corresponding to the target incident light after it passes through the mixed solution, obtaining a detection signal. The processor then determines the detection result based on the detection signal.

[0064] Optionally, before emitting incident light, the absorbance of the sample solution is determined, and the absorbance is greater than a preset threshold. In this embodiment, when the absorbance of the sample solution is greater than the preset threshold, the mixed solution is irradiated with target incident light. Due to the high background of the sample, the detection signal of the emitted light corresponding to the target incident light after passing through the mixed solution in the early stage of the coagulation reaction will be within the measurement range of the measuring device and will be greater than the detection signal of the emitted light corresponding to the mixed solution irradiated with normal incident light. Furthermore, according to the formula for calculating absorbance, when the difference between absorbance in the early stage of the coagulation reaction (i.e., the baseline period) and the difference between absorbance in the later stage of the coagulation reaction are the same, the higher the detection signal in the early stage of the coagulation reaction, the greater the change amplitude of the detection signal during the coagulation reaction. Therefore, when the background of the sample is too high, controlling the light source to emit target incident light can increase the change amplitude of the detection signal measured by the measuring device during the coagulation reaction. Furthermore, since the ratio of the increase in the detection signal is greater than the ratio of the increase in light source noise due to the increase in light intensity, the signal-to-noise ratio increases, thereby improving the repeatability and accuracy of the test results.

[0065] Optionally, when the target incident light is normal incident light and can be accurately measured (i.e., the measured signal-to-noise ratio is large, greater than 3.0), the above preset threshold is the maximum value of the sample absorbance.

[0066] For example, the formula for calculating absorbance (A) is as follows:

[0067]

[0068] The absorbance calculated by this formula is a dimensionless quantity, where T 入射光 T is the intensity of the incident light. 出射光 It is the light intensity value of the emitted light after passing through the sample solution.

[0069] Optionally, the sample analyzer has an interference detection channel. The processor controls the light source device to emit detection incident light, which is normal incident light. The detection signal of the emitted light is then acquired, and the absorbance of the sample solution is determined based on the detection signal of the emitted light. In this embodiment, the interference detection channel of the sample analyzer is used to determine the absorbance of the sample solution, and normal incident light is used as the detection incident light. When using normal incident light to detect the sample background, a more accurate incident light can be measured regardless of whether the sample background is too high. This avoids the light source device emitting target incident light to detect the sample background when the sample background is low, which would cause the detection signal obtained by the measuring device from the emitted light to exceed the measurement range.

[0070] Optionally, the sample analyzer has a first calibration channel, in which a light intensity attenuator is provided. The target incident light is calibrated based on the actual detection signal of the corresponding outgoing light after the target incident light passes through the first calibration channel. In this embodiment, the light intensity attenuator is used during calibration to reduce the light intensity value of the target incident light, so that the detection signal of the corresponding outgoing light after the target incident light passes through the light intensity attenuator is within the measurement range of the measuring device.

[0071] For example, the sample analyzer mentioned in this application embodiment has a total of 24 channels: one for normal light intensity calibration, one for high light intensity calibration, one for interfering substance detection, and the remaining 21 are ordinary detection channels. The first calibration channel mentioned above belongs to the high light intensity calibration channel. Light intensity calibration and detection of the mixed solution can be performed simultaneously. For example, during detection, calibration is performed every 100ms using either the high-intensity incident light or the normal-intensity incident light used in the ordinary detection channel. In some examples, the 21 ordinary detection channels can be used to perform various detection projects, such as coagulation method, colorimetric turbidimetric method, and chromogenic substrate method. Specifically, the change sensitivity wavelengths for the coagulation method are 660nm and 450nm, the change sensitivity wavelength for the colorimetric turbidimetric method is 575nm, and the change sensitivity wavelength for the chromogenic substrate method is 405nm.

[0072] Optionally, if the actual detected signal is greater than the target detected signal, the operating current of the light source device is reduced; if the actual detected signal is less than the target detected signal, the operating current of the light source device is increased. In this embodiment, the target incident light is calibrated based on the actual detected signal, that is, the operating current of the light source device is adjusted. The larger the operating current, the greater the light intensity of the target incident light emitted by the light source device; conversely, the smaller the operating current, the smaller the light intensity of the target incident light emitted by the light source device, until the actual detected signal is equal to or closest to the target detected signal. This makes the light intensity value of the target incident light more accurate, thereby improving the accuracy of the measurement results.

[0073] 502. Obtain the detection signal of the emitted light from the first target.

[0074] The first target emitted light referred to here is the emitted light corresponding to the target incident light after passing through the mixed solution in the early stage of the solidification reaction.

[0075] In this embodiment, the early stage of the coagulation reaction (i.e., the baseline period) is the part of the coagulation reaction curve where the AD value changes relatively smoothly. In some embodiments, the smoothness of the AD value change can be determined based on the magnitude of the AD value change, such as by using methods like difference, slope, or integration to identify the part where the AD value changes relatively smoothly at the beginning of the coagulation reaction. This segment can then be identified as the early stage of the coagulation reaction. In some embodiments, detection signals (AD values) at multiple time points can be collected within a certain window (the interval where the AD value changes relatively smoothly) in the early stage of the coagulation reaction, and the average value of these multiple AD values ​​can be used as the detection signal of the emitted light from the first target.

[0076] 503. Obtain the detection signal of the emitted light from the second target.

[0077] The second target emitted light referred to here is the emitted light corresponding to the target incident light after passing through the mixed solution in the later stage of the solidification reaction.

[0078] In this embodiment, the later stage of the solidification reaction is the part of the solidification reaction curve where the AD value changes relatively smoothly. In some embodiments, the smoothness of the AD value change can also be determined based on the magnitude of the AD value change. Methods such as difference, slope, or integration can be used to identify the part of the later stage of the solidification reaction where the AD value changes relatively smoothly. In some embodiments, after determining the later stage of the solidification reaction, all or part of the AD values ​​in this interval can be collected for calculation to obtain the detection signal of the emitted light from the second target, such as the average value. For example, the later stage of the solidification reaction is divided into 3 windows, and AD values ​​corresponding to 3 time points are randomly selected in each window. Then, the average value of these 9 AD values ​​is calculated as the detection signal of the emitted light from the second target.

[0079] 504. Determine the detection result of the sample based on the detection signal of the emitted light from the first target and the detection signal of the emitted light from the second target.

[0080] In some embodiments, the time point at which the coagulation reaction ends can be obtained based on the detection signals of the first target emitted light and the second target emitted light. For example, the time point corresponding to the midpoint between the detection signal obtained in the early stage of the coagulation reaction and the detection signal obtained in the later stage of the coagulation reaction is selected as the time point at which the coagulation reaction ends. This yields a coagulation reaction time from the start of the coagulation reaction (e.g., the time point after adding the last reagent to the mixed solution and then re-inserting it into the detection channel) to the aforementioned time point at which the coagulation reaction ends, thereby allowing the determination of the sample's detection result. Optionally, the sample concentration can be further obtained based on the aforementioned coagulation reaction time. The aforementioned coagulation reaction time and the time point at which the coagulation reaction ends can be found in [reference needed]. Figure 3 .

[0081] In this embodiment, the mixed solution is irradiated with target incident light, i.e., the light source device is adjusted to operate in high-intensity mode. In this mode, the signal-to-noise ratio of the measured results is relatively high. More specifically, the ratio of the intensity of the target incident light to the intensity of the normal incident light is greater than the ratio of the increase in noise caused by the increased intensity. This ratio is equal to the ratio of the change in the detection signal measured by the measuring device after the target incident light passes through the mixed solution during the solidification reaction to the change in the detection signal measured by the measuring device after the normal incident light passes through the mixed solution. Therefore, compared with irradiating the mixed solution with normal incident light, irradiating the mixed solution with target incident light yields a higher signal-to-noise ratio, thereby improving the repeatability and accuracy of the test results.

[0082] Please see Figure 6 , Figure 6 This is a schematic flowchart of another detection method provided in an embodiment of this application. For a description of the light source device and measuring device involved in the embodiments of this application, please refer to the above description. Figure 1 The details of the above process will not be elaborated here. The following will provide a detailed explanation of the steps involved in the above process.

[0083] 601. Determine the absorbance of the sample solution.

[0084] In some embodiments, the sample analyzer has an interference detection channel, whereby the processor controls a light source device to emit detection incident light, and the detection incident light is normal incident light. The emitted light (also referred to as "detection emitted light") corresponding to the detection incident light passing through the sample solution in the interference detection channel is then acquired. The absorbance of the sample solution is determined based on the detection signal of this detection emitted light.

[0085] Optionally, the incident light to be detected is normal incident light, the maximum value of which is the detection signal of the corresponding outgoing light after passing through the air is within the measurement range of the measuring device, and the detection signal of the corresponding outgoing light after passing through the air satisfies M times the full voltage range of the incident light, where M is a positive real number greater than 0.5 and less than 1, preferably 0.75.

[0086] For example, the aforementioned detection incident light can be the normal incident light from the first portion of the series of lights emitted by the light source device. For instance, the wavelength of the first incident light is 405 nm with an intensity of 26000, the wavelength of the second incident light is 405 nm with an intensity of 154000, the wavelength of the third incident light is 660 nm with an intensity of 26000, the wavelength of the fourth incident light is 660 nm with an intensity of 154000, and so on. In this case, the aforementioned detection incident light can be the first incident light. This allows the absorbance of the sample to be detected immediately after the light source device emits this series of lights, and thus, after the test, the target emitted light can be determined from the emitted light corresponding to the aforementioned series of lights.

[0087] 602. Based on the absorbance, determine the target incident light used to irradiate the mixture of sample and reagent.

[0088] The target incident light is one beam of light emitted by the light source device in the aforementioned series of lights, including a first incident light, a second incident light, etc. The target incident light is determined from this series of lights based on the absorbance of the sample. The light intensity of the first incident light is higher than that of the second incident light. For example, the light intensity and wavelength of the first and second incident lights can be different, which can be used for different detection items and detection methods, such as coagulation method, colorimetric turbidimetric method, chromogenic substrate method, etc. Among them, the change sensitivity wavelengths of the coagulation method are 660nm and 450nm, the change sensitivity wavelength of the colorimetric turbidimetric method is 575nm, and the change sensitivity wavelength of the chromogenic substrate method is 405nm.

[0089] Optionally, the light intensity value of the first incident light is N times the light intensity value of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the emitted light corresponding to the second incident light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

[0090] Optionally, if the absorbance is greater than a preset threshold, the target incident light is determined to be the first incident light; if the absorbance is less than the preset threshold, the target incident light is determined to be the second incident light.

[0091] Optionally, the sample analyzer has a first calibration channel, in which a light intensity attenuator is provided. When the target incident light is determined to be the first incident light, the processor obtains the actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel, and then calibrates the target incident light according to the actual detection signal.

[0092] Optionally, the sample analyzer has a second calibration channel. When the target incident light is determined to be the second incident light, the processor acquires the actual detection signal of the outgoing light corresponding to the target incident light after passing through the second calibration channel, and then calibrates the target incident light according to the actual detection signal.

[0093] For a detailed explanation of this step, please refer to the above text. Figure 5 The explanation of step 501 will not be detailed here.

[0094] 603. Control the light source device to emit incident light.

[0095] The aforementioned incident light includes at least a portion of the target incident light.

[0096] Optionally, the sample analyzer includes multiple detection channels. The light source device, under the control of the processor, switches different wavelengths of the incident light during the detection process, determining the time segment of the target incident light within the incident light based on the detection items of the detection channels and the target incident light. Because the aforementioned incident light is a series of lights with different intensities and wavelengths, and a portion of this light is the target incident light, the target incident light will occupy a time segment of the incident light.

[0097] 604. Obtain the detection signal of the first emitted light.

[0098] Optionally, the detection signals of the first and second emitted light can be determined based on the time segment.

[0099] For a detailed explanation of this step, please refer to the above text. Figure 5 The explanation of step 502 will not be detailed here.

[0100] 605. Obtain the detection signal of the second emitted light.

[0101] Optionally, the detection signals of the first and second emitted light can be determined based on the time segment.

[0102] For a detailed explanation of this step, please refer to the above text. Figure 5 The explanation of step 503 will not be detailed here.

[0103] 606. Determine the detection result of the sample solution based on the detection signals of the first emitted light and the second emitted light. Optionally, when the sample absorbance is greater than the threshold and the target incident light is the second incident light, after acquiring the detection signals of the first emitted light and the second emitted light, amplify the detection signals of the first emitted light and the second emitted light (i.e., high-gain mode), at which point the signal-to-noise ratio is larger.

[0104] Optionally, when the sample absorbance is less than the threshold and the target incident light is the second incident light, the measurement result (i.e., normal light intensity mode) can be obtained directly after acquiring the detection signal of the first outgoing light and the detection signal of the second outgoing light.

[0105] As an example, with a sample background of 2 and a sample coagulation reaction absorbance variation of 0.02, measurements were performed using normal intensity mode, high gain mode, and high intensity mode, respectively. The measurement data are shown in the table below:

[0106]

[0107] In the table above, the initial AD is the AD obtained by the measuring device directly measuring the light signal emitted by the light source device. The AD in the early stage of the reaction is the AD obtained by the measuring device measuring the light signal after passing through the mixed solution in the early stage of the mixed solution solidification reaction. The AD after the reaction is completed is the AD obtained by the measuring device measuring the light signal after passing through the mixed solution in the later stage of the mixed solution solidification reaction. The AD change amplitude is the difference between the AD in the early stage of the reaction and the AD in the later stage of the reaction. This AD change amplitude is the useful signal of the measurement result.

[0108] As shown in the chart, since the background of the mixed solution is 2, which is greater than the preset threshold of 1.5, indicating a high concentration of interfering substances in the mixed solution, the signal-to-noise ratio (SNR) after signal processing is 6.93 when the light source device operates in high-intensity mode. This is greater than the SNR after signal processing when the light source device operates in normal-intensity mode (3.90) and the SNR after signal processing when the measuring device operates in high-gain mode (5.57). More specifically, the ratio of the AD change amplitude in high-intensity mode to that in normal-intensity mode (69.3 / 11.7) is equal to the initial AD ratio (154000 / 26000), equal to the AD ratio in the early stage of the solidification reaction (1540 / 260), and greater than the noise ratio after signal processing (10 / 3). In other words, during the solidification reaction, the proportion of the increase in light signal intensity by the light source device is equal to the proportion of the increase in AD change amplitude due to the increase in light intensity, and greater than the proportion of the increase in light source noise due to the increase in light source signal intensity. Therefore, when the background of the mixed solution is high, adjusting the light source to operate in high light intensity mode can effectively improve the signal-to-noise ratio of the measurement, thereby solving the problem of measurement deviation caused by the presence of interfering substances in the mixed solution, and thus improving the accuracy of the measurement results.

[0109] It is evident that both high-gain and high-intensity modes can improve the signal-to-noise ratio (SNR), with the high-intensity mode being more effective. For mixed solutions with high concentrations of interfering substances, the high-intensity mode should be prioritized. However, for normal mixed solutions (or mixed solutions with a background below a preset threshold), the signal falls outside the measurement board's acquisition range under high-intensity mode. Therefore, high-intensity mode is not suitable for normal mixed solutions; instead, high-gain mode is more appropriate.

[0110] In this embodiment, the target incident light is determined based on the absorbance of the sample solution. The target incident light is one of the first incident light and the second incident light, which makes the light intensity value of the incident light irradiating the mixed solution more flexible and can improve the accuracy of the measurement results.

[0111] Please see Figure 7 , Figure 7 This is a schematic flowchart of another detection method provided in the embodiments of this application. For a description of the light source device and measuring device involved in the embodiments of this application, please refer to the above description. Figure 1 The details of the above process will not be elaborated here. The following will provide a detailed explanation of the steps involved in the above process.

[0112] 701. Determine the absorbance of the sample.

[0113] For a detailed explanation of this step, please refer to the above text. Figure 5 The explanation of step 501 will not be detailed here.

[0114] 702. Determine whether the absorbance is greater than the preset threshold. If the absorbance is greater than the preset threshold, proceed to step 703. If the absorbance is less than the preset threshold, proceed to step 704.

[0115] 703. Control the light source device to operate in high light intensity mode.

[0116] For a detailed explanation of this step, please refer to the above text. Figure 5 The explanations of steps 501, 502, and 503 will not be detailed here.

[0117] 704. Control the light source device to operate in normal light intensity mode.

[0118] For a detailed explanation of this step, please refer to the above text. Figure 6 The explanations for steps 603, 604, and 605 will not be detailed here.

[0119] 705. The control and measurement device operates in high-gain mode.

[0120] For a detailed explanation of the high-gain mode, please refer to the section above. Figure 6 The explanation of step 606 will not be detailed here.

[0121] Optionally, when the sample absorbance is less than the threshold and the target incident light is the second incident light, the measurement result can be obtained directly after acquiring the detection signal of the first outgoing light and the detection signal of the second outgoing light (i.e., normal light intensity mode), without controlling the measurement device to work in high gain mode.

[0122] The device provided in the embodiments of this application will be described below.

[0123] This application divides the device into functional modules based on the above method embodiments. For example, each function can be divided into its own functional modules, or two or more functions can be integrated into one processing module. The integrated modules can be implemented in hardware or as software functional modules. It should be noted that the module division in this application is illustrative and represents only one logical functional division; other division methods may be used in actual implementation. The following will combine... Figure 8 The device according to the embodiments of this application is described in detail.

[0124] Please see Figure 8 , Figure 8This is a schematic diagram of a sample analyzer provided in an embodiment of this application. The sample analyzer includes a processor 801, a light source device 802, a measuring device 103, and a detection channel 804. The processor 801 is connected to the light source device 802 and the measuring device 103, for example, through electrical or communication connections, and is used to control the light source device 802 and the measuring device 103 to obtain detection results. The light source device 802 is used to provide incident light with at least different intensities. The measuring device 103 is used to detect the detection signal of the emitted light generated after the incident light passes through the reaction cup containing the mixed solution. The detection channel 804 is at least partially used to hold the reaction cup containing the mixed solution of sample and reagent. At least one detection channel is required in the sample analyzer. It should be noted that... Figure 8 The processor, light source device, measuring device, and detection channel shown are merely examples. For the specific functions or execution steps of the processor, light source device, and measuring device, please refer to the above method embodiments, which will not be described in detail here.

[0125] This application also provides a computer-readable storage medium storing a computer program for electronic data interchange, which causes a computer to perform some or all of the steps of any of the methods for implementing key functions as described in the above method embodiments.

[0126] It should be noted that, for the sake of simplicity, the foregoing method embodiments are all described as a series of actions. However, those skilled in the art should understand that this application is not limited to the described order of actions, as some steps may be performed in other orders or simultaneously according to this application. Furthermore, those skilled in the art should also understand that the embodiments described in the specification are preferred embodiments, and the actions and modules involved are not necessarily essential to this application.

[0127] Those skilled in the art will understand that all or part of the steps in the various methods of the above embodiments can be implemented by a program instructing related hardware. The program can be stored in a computer-readable storage device, which may include: a flash drive, a read-only memory, a random access memory, a magnetic disk, or an optical disk, etc.

[0128] The embodiments of this application have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this application. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A detection method applied to a sample analyzer, characterized in that, The sample analyzer includes a processor, a light source device, a first calibration channel, and a measuring device; the light source device is used to provide incident light with different light intensities under the control of the processor; the measuring device is used to detect the detection signal of the corresponding emitted light after the incident light passes through a reaction cup containing a mixed solution of sample and reagent; The first calibration channel is equipped with a light intensity attenuator; the detection method includes: The light source device is controlled to emit incident light, at least partially including target incident light. The first calibration channel is used to calibrate the light intensity value of the target incident light. The incident light includes a first incident light and a second incident light. The light intensity value of the first incident light is higher than that of the second incident light. The wavelengths of the first incident light and the second incident light are both sensitive wavelengths corresponding to the same detection item. Acquire the detection signal of the first target emitted light, wherein the first target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution in the early stage of the coagulation reaction; Acquire the detection signal of the second target emitted light, wherein the second target emitted light is the emitted light corresponding to the target incident light after passing through the mixed solution in the later stage of the coagulation reaction; The detection result of the sample is determined based on the detection signal of the emitted light from the first target and the detection signal of the emitted light from the second target; Wherein, the light intensity value of the target incident light is N times the light intensity value of the normal incident light, where N is a positive real number greater than 5; the normal incident light is the incident light whose detection signal of the corresponding outgoing light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

2. The method according to claim 1, characterized in that, The value of N is in the range of [5.5, 6.5].

3. The method according to claim 1, characterized in that, Before the light source device emits incident light, the method further includes: The absorbance of the sample solution is determined, and the absorbance is greater than a preset threshold.

4. The method according to claim 3, characterized in that, The sample analyzer has an interference detection channel; The determination of the absorbance of the sample solution includes: The light source device is controlled to emit detection incident light, which is normal incident light; Acquire the detection signal of the emitted light, wherein the emitted light is the emitted light that passes through the sample solution in the interference detection channel; The absorbance of the sample solution is determined based on the detection signal of the emitted light.

5. The method according to any one of claims 1 to 4, characterized in that, The method further includes: Obtain the actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel; The incident light on the target is calibrated based on the actual detection signal.

6. The method according to claim 5, characterized in that, The step of calibrating the target incident light based on the actual detection signal includes: If the actual detection signal is greater than the target detection signal, then the operating current of the light source device is reduced. If the actual detection signal is less than the target detection signal, then the operating current of the light source device is increased.

7. A sample analyzer, characterized in that, include: At least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of sample and reagent; A light source device is used to provide incident light with different light intensity values. The incident light includes a first incident light and a second incident light. The light intensity value of the first incident light is higher than that of the second incident light. The wavelengths of the first incident light and the second incident light are both sensitive wavelengths corresponding to the same detection item. A measuring device is used to detect the detection signal of the emitted light generated after the incident light passes through the reaction vessel of the mixed solution; The first calibration channel is used to calibrate the light intensity value of the incident light from the target. A processor, connected to the light source device and the measuring device, and configured to perform the method as described in any one of claims 1 to 6.

8. A detection method applied to a sample analyzer, characterized in that, The sample analyzer includes a processor, a light source device, a first calibration channel, and a measuring device; the light source device is used to provide incident light with different light intensities under the control of the processor; the measuring device is used to detect the detection signal of the corresponding emitted light after the incident light passes through a reaction cup containing a mixed solution of sample and reagent; The first calibration channel is equipped with a light intensity attenuator; the detection method includes: Determine the absorbance of the sample solution; Based on the absorbance, a target incident light for irradiating the sample and reagent mixture is determined, wherein the target incident light is one of a first incident light and a second incident light, the intensity of the first incident light is higher than the intensity of the second incident light, the intensity of the first incident light is N times the intensity of the second incident light, where N is a positive real number greater than 5; wherein the detection signal of the emitted light corresponding to the second incident light after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1; the first calibration channel is used to calibrate the intensity of the target incident light, and the wavelengths of the first and second incident lights are both sensitive wavelengths corresponding to the same detection item. The light source device is controlled to emit incident light, the incident light including at least a portion of the target incident light; Acquire the detection signal of the first emitted light, wherein the first emitted light is the emitted light corresponding to the mixed solution in the early stage of the coagulation reaction after the target incident light irradiates it; Acquire the detection signal of the second emitted light, wherein the second emitted light is the emitted light corresponding to the mixed solution in the later stage of the coagulation reaction after the target incident light irradiates it; The detection result of the sample solution is determined based on the detection signal of the first emitted light and the detection signal of the second emitted light.

9. The method according to claim 8, characterized in that, The step of determining the target incident light for irradiating the sample and reagent mixture based on the absorbance includes: If the absorbance is greater than a preset threshold, then the target incident light is determined to be the first incident light; If the absorbance is less than a preset threshold, then the target incident light is determined to be the second incident light.

10. The method according to claim 8, characterized in that, The method further includes: When the target incident light is determined to be the first incident light, the actual detection signal of the outgoing light corresponding to the target incident light after passing through the first calibration channel is obtained. The incident light on the target is calibrated based on the actual detection signal.

11. The method according to claim 8, characterized in that, The sample analyzer has a second calibration channel; the method further includes: When the target incident light is determined to be the second incident light, the actual detection signal of the outgoing light corresponding to the target incident light after passing through the second calibration channel is obtained; The incident light on the target is calibrated based on the actual detection signal.

12. The method according to claim 8, characterized in that, The sample analyzer has an interference detection channel; The determination of the absorbance of the sample solution includes: The light source device is controlled to emit detection incident light, which is normal incident light; Acquire the detection signal of the emitted light, wherein the emitted light is the emitted light corresponding to the incident light after passing through the sample solution in the interference detection channel; The absorbance of the sample solution is determined based on the detection signal of the emitted light.

13. The method according to claim 12, characterized in that, The incident light to be detected is normal incident light, wherein the normal incident light is the incident light whose detection signal of the corresponding outgoing light after passing through the air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1.

14. The method according to claim 8, characterized in that, The sample analyzer includes multiple detection channels; the light source device is used to switch different wavelengths of the incident light during the detection process under the control of the processor; the method further includes: Based on the detection items of the detection channel and the target incident light, determine the time segment of the target incident light in the incident light; Based on the time segment, the detection signals of the first emitted light and the second emitted light are determined.

15. The method according to claim 8, characterized in that, After determining that the target incident light is the second incident light, the steps of acquiring the detection signal of the first emitted light and acquiring the detection signal of the second emitted light include: The detection signals of the first emitted light and the second emitted light are amplified.

16. A sample analyzer, characterized in that, include: At least one detection channel, at least a portion of which is used to load a reaction cup containing a mixed solution of sample and reagent; A light source device is used to provide incident light with different light intensity values. The incident light includes a first incident light and a second incident light. The light intensity value of the first incident light is higher than that of the second incident light. The light intensity value of the first incident light is N times that of the second incident light, where N is a positive real number greater than 5. The detection signal of the emitted light corresponding to the second incident light after passing through air is within the measurement range of the measuring device and satisfies M times the full voltage range, where M is a positive real number greater than 0.5 and less than 1. The wavelengths of the first incident light and the second incident light are both sensitive wavelengths corresponding to the same detection item. A measuring device is used to detect the detection signal of the emitted light generated after the incident light passes through the reaction vessel of the mixed solution; The first calibration channel is used to calibrate the light intensity value of the incident light from the target. A processor, connected to the light source device and the measuring device, is used to perform the method as described in any one of claims 8 to 15.

Images