Efficient germination method of akebia trifoliate seeds
Through an efficient germination method that includes steps such as preparation, cleaning, removing seed coat and daily management, the problems of complex, late and low germination rate of Sanye Mutong seeds are solved, and 100% germination rate and short-term germination are achieved, supporting the large-scale reproduction of Sanye Mutong.
Patent Information
- Application Number
- CN202510259536.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-06
- Publication Date
- 2025-04-15
AI Technical Summary
The treatment of Sanye Mutong seeds is complicated before germination, has late germination, long germination time, and low germination rate, making it difficult to achieve large-scale reproduction.
An efficient germination method is adopted, including steps: preparing seeds, cleaning seeds, removing seed coats, seed germination and daily management. These steps can improve the germination rate and speed of seeds.
The 100% germination rate of Sanye Mutong seeds was achieved, with early germination (12 days) and short germination time (16 days), providing a technical basis for the large-scale reproduction of Sanye Mutong.
Smart Images
Figure CN119817235A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of plant breeding, and particularly relates to a germination method for Akebia trifoliata seeds. Background Art
[0002] Akebia trifoliata (Thunb.) Koidz. is a deciduous woody vine belonging to the genus Akebia of the family Akebia. The fruit is oblong, straight or slightly curved, and grayish white with a slight lavender color when mature. There are very many seeds, which are flat and ovate, with a reddish brown or dark brown seed coat and a slight luster. The flowering period is April-May, and the fruiting period is July-August. The roots, stems and fruits of Akebia trifoliata are all used as medicine, which can promote diuresis and lactation, relax muscles and tendons, and treat rheumatic joint pain; the fruit can also be eaten and used for winemaking; the seeds can be used to extract oil. However, the seeds are sensitive to dehydration and are stubborn seeds. The self-propagation ability of Akebia trifoliata seeds is poor, and seedlings are rarely seen in its distribution area. A review of the literature found that the germination of Akebia trifoliata seeds requires pre-treatment before germination. Summary of the invention
[0003] The invention aims at the problems of complicated pre-germination treatment, late germination and long germination time of Akebia trifoliata seeds, and provides an efficient germination method for Akebia trifoliata seeds. The method is simple to operate, low in cost, early in germination (12 days), short in germination time (16 days), and 100% in germination rate, thus providing a technical basis for large-scale reproduction and application of Akebia trifoliata.
[0004] To solve the above problems, the technical solution of the present invention is: a high-efficiency germination method for Akebia trifoliata seeds, characterized in that it comprises the following steps:
[0005] Step 1: Prepare the seeds
[0006] Pick fresh, mature, and unopened Akebia trifoliata fruits, break open the outer peel of the fruit along the cracking line, scoop out the pulp and seeds with a spoon, and put them into a basin filled with tap water;
[0007] Step 2: Clean the seeds
[0008] Wash the seeds repeatedly by hand in a basin of water, stir the liquid mixed with pulp, seeds and tap water with a small fishing net, fish out the pulp separated from the seeds with a small fishing net and throw it away, then fish out the seeds and seeds with pulp with a small fishing net, pour out the tap water in the basin, then put the fished seeds and seeds with pulp into a basin and add tap water, continue to wash repeatedly by hand, repeat the above seed cleaning process 3-6 times until clean seeds are obtained, and this step is completed within 2 hours;
[0009] Step 3: Remove the seed coat
[0010] Take the cleaned seeds, hold the seeds with one hand, and the white hard appendages around the seed hilum with the other hand, break off the white hard appendages, tear off the rest of the seed coat with your fingernails, then add tap water to wash the seeds with the seed coat torn off, and use a small fish net to fish out the seeds with the seed coat to set aside;
[0011] Step 4: Seed germination
[0012] Put the cleaned and peeled seeds into a transparent ziplock bag, add 3 mL of purified water, seal part of the bag, fill the bag with air using a handheld air pump, seal the bag, and place it in a 10°C refrigerator for germination;
[0013] Step 5: Daily Management
[0014] Open the transparent ziplock bag every 2-3 days, pour the seeds into the basin, add purified water and stir repeatedly with a small fishing net, then use the small fishing net to pick out the seeds, rub and rinse the original transparent ziplock bag with your hands, then put the seeds back into the original transparent ziplock bag, use an air pump to fill the transparent ziplock bag with air, seal the transparent ziplock bag and continue germination;
[0015] Step 6: Post-germination treatment
[0016] Check the seeds in the transparent ziplock bag every day. When you find germinated seeds, open the transparent ziplock bag, pick them up with tweezers, and then seal the bag. The remaining seeds continue to germinate until no seeds germinate for 5 consecutive days, and the germination is over.
[0017] The present invention has the following obvious effects
[0018] 1. This method is simple and easy, low-cost, and has obvious germination effect. The germination rate of seeds reaches 100%.
[0019] 2. Akebia trifoliata seeds are stubborn seeds, very sensitive to water, difficult to preserve, and difficult to germinate. This method reasonably controls the moisture of the seeds, maintains the moisture and oxygen required for seed germination, and the seeds achieve 100% germination effect.
[0020] 3. Compared with the traditional sand storage germination, the seeds of this method germinate earlier (12 days), the germination time is shorter (16 days), and the germination effect is better (germination rate 100%), providing a technical basis for the seed propagation of Akebia trifoliata. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is the fruit of Akebia trifoliata;
[0022] Figure 2 It is the seed of Akebia trifoliata;
[0023] Figure 3 It is a white hard appendage around the hilum;
[0024] Figure 4 It is the seeds in the ziplock bag that begin to germinate;
[0025] Figure 5 It is ziplock bag seed germination;
[0026] Figure 6 It is the germination of the seeds stored in the sand;
[0027] Figure 7 It is the germination of seeds with seed coat;
[0028] Figure 8 The seeds are germinated by removing the seed coat. DETAILED DESCRIPTION
[0029] Fruit cracking line: When the fruit of Akebia trifoliata is ripe, it will crack along the longitudinal arc on the surface of the fruit. This arc is called the cracking line.
[0030] Stubborn seeds: refers to seeds that are not tolerant to water loss and should avoid dryness and low temperatures during storage.
[0031] An efficient germination method for Akebia trifoliata seeds comprises the following steps:
[0032] Step 1: Prepare the seeds
[0033] Pick fresh, ripe, and uncracked Akebia fruits (fruit rot will affect seed germination), break open the fruit's outer peel along the fruit's cracking line with your hands, scoop out the pulp and seeds with a spoon, and put them in a basin of tap water (the water mentioned below refers to tap water unless otherwise specified).
[0034] Step 2: Clean the seeds
[0035] Rub the seeds repeatedly in a basin of water with your hands, stir the liquid mixed with pulp, seeds and water with a small fishing net (fish tank fishing net), use the small fishing net to scoop out the pulp separated from the seeds and throw it away, then use the small fishing net to scoop out the seeds and seeds with pulp, pour out the water in the basin, then put the scooped seeds and seeds with pulp into the basin and add water, continue to rub them repeatedly with your hands, repeat the above seed cleaning process (3-6 times) until clean seeds are obtained. The seed cleaning operation should be completed within 2 hours as much as possible.
[0036] Step 3: Remove the seed coat
[0037] Take the cleaned seeds, hold the seeds in your left hand, pinch the white hard appendages around the hilum of the seeds with your right hand, break off the white hard appendages, tear off the rest of the seed coat with your fingernails, then add water to rub the seeds with the seed coat torn off, use a small fish net to remove the seeds with the seed coat removed and set aside.
[0038] Step 4: Seed germination
[0039] Put the cleaned and peeled seeds into a 120X170mm ziplock bag, add 3mL of purified water, seal part of the bag, fill the ziplock bag with air using a handheld air pump, then seal the ziplock bag and place it in a 10℃ refrigerator for germination.
[0040] Step 5: Daily Management
[0041] Open the ziplock bag every 2-3 days, pour the seeds into the basin, add purified water and stir repeatedly with a small fishing net, then use the small fishing net to scoop out the seeds. Wash and rinse the original ziplock bag by hand, then put the seeds into the original ziplock bag, use an air pump to fill the ziplock bag with air, seal it and continue germination.
[0042] Step 6: Post-germination treatment
[0043] Check the seeds in the ziplock bag every day. When you find germinated seeds, open the ziplock bag, pick them up with tweezers, and then seal the bag. The remaining seeds continue to germinate until no seeds germinate for 5 consecutive days, and the germination is over.
[0044] Example 1
[0045] Experimental location: Office building of the old park of Xi'an Botanical Garden, Shaanxi Province
[0046] Experimental period: September-November 2024
[0047] Source of experimental materials: Akebia trifoliata fruits were obtained from Huaihua City, Hunan Province
[0048] Step 1: Prepare the seeds
[0049] Pick fresh, ripe, and uncracked Akebia fruits (fruit rot will affect seed germination), break open the fruit's outer peel along the fruit's cracking line with your hands, scoop out the pulp and seeds with a spoon, and put them in a basin of tap water (the water mentioned below refers to tap water unless otherwise specified).
[0050] Step 2: Clean the seeds
[0051] Rub the seeds repeatedly in a basin of water with your hands, stir the liquid mixed with pulp, seeds and water with a small fishing net (fish tank fishing net), use the small fishing net to scoop out the pulp separated from the seeds and throw it away, then use the small fishing net to scoop out the seeds and seeds with pulp, pour out the water in the basin, then put the scooped seeds and seeds with pulp into the basin and add water, continue to rub them repeatedly with your hands, repeat the above seed cleaning process (3-6 times) until clean seeds are obtained. The seed cleaning operation should be completed within 2 hours as much as possible.
[0052] Step 3: Remove the seed coat
[0053] Take the cleaned seeds, hold the seeds in your left hand, pinch the white hard appendages around the hilum of the seeds with your right hand, break off the white hard appendages, tear off the rest of the seed coat with your fingernails, then add water to rub the seeds with the seed coat torn off, use a small fish net to remove the seeds with the seed coat removed and set aside.
[0054] Step 4: Seed germination
[0055] Put the cleaned and peeled seeds into a ziplock bag, add 3 mL of pure water, seal part of the bag, fill the ziplock bag with air using a handheld air pump, then seal the ziplock bag and place it in a 10°C refrigerator for germination.
[0056] Step 5: Daily Management
[0057] Open the ziplock bag every 2-3 days, pour the seeds into the basin, add purified water and stir repeatedly with a small fishing net, then use the small fishing net to scoop out the seeds. Wash and rinse the original ziplock bag by hand, then put the seeds back into the original ziplock bag, use an air pump to fill the ziplock bag with air, and continue germination.
[0058] Step 6: Post-germination treatment
[0059] Check the seeds in the transparent ziplock bag every day. When you find the germinated seeds, open the ziplock bag, pick them up with tweezers, and then seal the bag. The remaining seeds continue to germinate until there are no germinated seeds for 5 consecutive days. Germination is over. (See Table 3 for test results)
[0060] Example 2
[0061] Experimental location: Office building of the old park of Xi'an Botanical Garden, Shaanxi Province
[0062] Experimental period: September-November 2024
[0063] Source of experimental materials: Akebia trifoliata fruits were from Fujian Province
[0064] Step 1: Prepare the seeds
[0065] Pick fresh, ripe, and uncracked Akebia fruits (fruit rot will affect seed germination), break open the fruit's outer peel along the fruit's cracking line with your hands, scoop out the pulp and seeds with a spoon, and put them in a basin of tap water (the water mentioned below refers to tap water unless otherwise specified).
[0066] Step 2: Clean the seeds
[0067] Rub the seeds repeatedly in a basin of water with your hands, stir the liquid mixed with pulp, seeds and water with a small fishing net (fish tank fishing net), use the small fishing net to scoop out the pulp separated from the seeds and throw it away, then use the small fishing net to scoop out the seeds and seeds with pulp, pour out the water in the basin, then put the scooped seeds and seeds with pulp into the basin and add water, continue to rub them repeatedly with your hands, repeat the above seed cleaning process (3-6 times) until clean seeds are obtained. The seed cleaning operation should be completed within 2 hours as much as possible.
[0068] Step 3: Remove the seed coat
[0069] Take the cleaned seeds, hold the seeds in your left hand, pinch the white hard appendages around the hilum of the seeds with your right hand, break off the white hard appendages, tear off the rest of the seed coat with your fingernails, then add water to rub the seeds with the seed coat torn off, use a small fish net to remove the seeds with the seed coat removed and set aside.
[0070] Step 4: Seed germination
[0071] Put the cleaned and peeled seeds into a ziplock bag, add 3 mL of pure water, seal part of the bag, fill the ziplock bag with air using a handheld air pump, then seal the ziplock bag and place it in a 10°C refrigerator for germination.
[0072] Step 5: Daily Management
[0073] Open the ziplock bag every 2-3 days, pour the seeds into the basin, add purified water and stir repeatedly with a small fishing net, then use the small fishing net to scoop out the seeds. Wash and rinse the original ziplock bag by hand, then put the seeds back into the original ziplock bag, use an air pump to fill the ziplock bag with air, and continue germination.
[0074] Step 6: Post-germination treatment
[0075] Check the seeds in the ziplock bag every day. When you find the germinated seeds, open the ziplock bag, pick them up with tweezers, and then seal the bag. The remaining seeds continue to germinate until there are no germinated seeds for 5 consecutive days. Germination is over. (See Table 4 for test results)
[0076] According to the literature, after the seeds were stored in sand at room temperature, the germination rate reached 83% after germination for 20 days; after the seeds were stratified at 5°C for 50 days, the germination rate was 92%; after the seeds were stratified in wet sand for 30 days, the germination rate was 80.87% at 16°C. The results of germination in sand storage and transparent ziplock bags are shown in Table 1, the results of germination in transparent ziplock bags at different temperatures are shown in Table 2, and the results of germination in ziplock bags with seed coat removed are shown in Table 3 and Table 4.
[0077] Table 1 Seed germination by different methods
[0078]
[0079] Table 1 shows that under 25°C, the germination rate of seeds in ziplock bags is higher than that in sand storage.
[0080] Table 2 Seed germination at different temperatures
[0081]
[0082] Table 2 shows that the average germination rate of seeds in transparent ziplock bags at 10°C is 93.87%, which is the highest average germination rate.
[0083] Table 3 Germination of seeds without seed coat (2024)
[0084]
[0085] Table 4 Germination of seeds without seed coat (2024)
[0086]
Claims
1. A highly efficient germination method for Akebia trifoliata seeds, characterized in that: The following steps are involved: Step 1: Prepare the seeds Pick fresh, mature, and unopened Akebia trifoliata fruits, break open the outer peel of the fruit along the cracking line, scoop out the pulp and seeds with a spoon, and put them into a basin filled with tap water; Step 2: Clean the seeds Wash the seeds repeatedly by hand in a basin of water, stir the liquid mixed with pulp, seeds and tap water with a small fishing net, fish out the pulp separated from the seeds with a small fishing net and throw it away, then fish out the seeds and seeds with pulp with a small fishing net, pour out the tap water in the basin, then put the fished seeds and seeds with pulp into a basin and add tap water, continue to wash repeatedly by hand, repeat the above seed cleaning process 3-6 times until clean seeds are obtained, and this step is completed within 2 hours; Step 3: Remove the seed coat Take the cleaned seeds, hold the seeds with one hand, and the white hard appendages around the seed hilum with the other hand, break off the white hard appendages, tear off the rest of the seed coat with your fingernails, then add tap water to wash the seeds with the seed coat torn off, and use a small fish net to fish out the seeds with the seed coat to set aside; Step 4: Seed germination Put the cleaned and peeled seeds into a transparent ziplock bag, add 3 mL of purified water, seal part of the bag, fill the bag with air using a handheld air pump, seal the bag, and place it in a 10°C refrigerator for germination; Step 5: Daily Management Open the transparent ziplock bag every 2-3 days, pour the seeds into the basin, add purified water and stir repeatedly with a small fishing net, then use the small fishing net to pick out the seeds, rub and rinse the original transparent ziplock bag with your hands, then put the seeds back into the original transparent ziplock bag, use an air pump to fill the transparent ziplock bag with air, seal the transparent ziplock bag and continue germination; Step 6: Post-germination treatment Check the seeds in the transparent ziplock bag every day. When you find germinated seeds, open the transparent ziplock bag, pick them up with tweezers, and then seal the bag. The remaining seeds continue to germinate until no seeds germinate for 5 consecutive days, and the germination is over.