Cutting seedling raising method for chieh-qua

Abstract

The invention provides a chieh-qua cutting seedling raising method which comprises the following steps: selecting chieh-qua seedlings with growing points reserved as cutting branches, performing soaking and root promoting pretreatment by using a mixed solution of glucose and indolebutyric acid, then performing matrix cutting, and performing subsequent management measures such as temperature, humidity, light, seedling hardening and the like. The method is simple and easy to implement, the survival rate of cutting seedlings is high, and the method has high utilization value for production and breeding.

Description

Technical Field

[0001] The invention relates to a cutting seedling raising method for zucchini. Background Art

[0002] Wax gourd is a variety of wax gourd in the Cucurbitaceae family. In China, wax gourd was originally planted in Guangdong, Hainan, Fujian and other places. In recent years, with the large-scale promotion of wax gourd, many scientific research and breeding units have stepped up and accelerated the selection and breeding of new varieties of wax gourd. However, during the breeding process, after the seedlings are planted, the breeding materials are prone to diseases such as damping-off and basal rot due to weather, environment and improper management, which has caused a devastating blow to some scarce breeding materials. Cutting seedling technology can quickly reproduce scarce breeding materials, thereby saving scarce breeding materials and accelerating the breeding process. Summary of the invention

[0003] The technical problem to be solved by the invention is to provide a cutting seedling raising method for kiwano, which is simple and easy to implement, has a high survival rate of cutting seedling raising and has high utilization value for production and breeding.

[0004] In order to solve the above technical problems, the technical solution of the present invention is:

[0005] A cutting seedling raising method for kiwifruit comprises the following steps:

[0006] S1. Sterilize the seedling medium thoroughly with disinfectant and then place it in the seedling tray for later use;

[0007] S2. Select 2-3 leaves of zucchini seedlings, cut off the main branches of zucchini seedlings below the root with scissors, remove some leaves on the upper part of zucchini seedlings, and retain 1-2 leaves and growth points as cuttings for standby;

[0008] S3. The lower end of the cuttings obtained in step S2 is immersed in the pretreatment solution and removed after 50-70 minutes;

[0009] S4. The cuttings processed in step S3 are inserted into the seedling medium in the seedling tray obtained in step S1;

[0010] S5. Build an arch shed, water the seedling tray after the cutting in step S4 and place it in the arch shed, cover the arch shed with a film and a shade net for heat preservation, moisture retention, and shade hardening; after 3-4 days of hardening, start semi-ventilation; after 5-7 days of hardening, shade around noon, and remove the shade net for the rest of the time; after 8-9 days of hardening, remove the film and shade net for daily management; on the 3rd and 6th days after cutting, spray the cuttings with disinfectant twice; 10-12 days after cutting, apply water-soluble fertilizer once;

[0011] S6. 16-19 days after cuttings, adventitious roots will grow at the bottom of the cuttings, and the cuttings will survive.

[0012] Furthermore, in step S1 of the present invention, the seedling medium is the golden No. 3 seedling medium purchased from Hangzhou Jinhai Agricultural Technology Co., Ltd.; the disinfectant is an 800-1000 times aqueous solution of carbendazim; and the seedling tray is a 32-hole seedling tray with a diameter of 6 cm and a depth of 5 cm.

[0013] Furthermore, in step S2 of the present invention, the scissors are disinfected with an ethanol aqueous solution with a volume concentration of 75% before use; and the length of the cuttings is 15-20 cm.

[0014] Furthermore, in step S3 of the present invention, the pretreatment solution is formed by mixing an aqueous glucose solution with a concentration of 8 g / L and an aqueous indolebutyric acid solution with a concentration of 50 mg / L in a volume ratio of 1:1; and the immersion length of the cuttings is 3-5 cm.

[0015] Furthermore, in step S4 of the present invention, the cutting depth of the cuttings in the seedling medium is 3-4 cm.

[0016] Furthermore, in step S5 of the present invention, during the heat preservation, moisture retention, shading and seedling hardening treatment, the temperature in the arch shed is 20-35° C., and the relative humidity of the air is ≥80%.

[0017] Furthermore, in step S5 of the present invention, the specific operation of semi-ventilation is: lifting two-thirds of the film on one side of the arch shed for ventilation.

[0018] Furthermore, in step S5 of the present invention, the shading time around noon is from 9:00 to 16:00.

[0019] Furthermore, in step S5 of the present invention, the specific operations of daily management are: daily natural light irradiation, the temperature in the arch shed is below 35°C during the day, the temperature in the arch shed is above 20°C at night, and the seedling medium is kept moist.

[0020] Furthermore, in step S5 of the present invention, the disinfectant is an 800-1000 times aqueous solution of carbendazim; the water-soluble fertilizer is a special fertilizer for the growth period with a nitrogen, phosphorus and potassium content of 20-10-20 purchased from Hongyue Flower Co., Ltd., which is diluted 500 times before application.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The invention selects the zucchini seedlings with the growth points retained as the cutting branches, soaks them in a mixed solution of glucose and indolebutyric acid for rooting pretreatment, then performs substrate cutting, and performs subsequent management measures such as temperature, humidity, light, and seedling hardening. The zucchini cutting seedlings treated by the invention have a large rooting amount and a survival rate of more than 95%. Practice has proved that the invention has low cost, is simple and easy to implement, has high utilization value for production and breeding, and has high application value for precious zucchini breeding materials in which the zucchini seedlings encounter diseases such as damping-off disease and basal rot in the seedbed or after planting, resulting in large-scale death of the seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present invention, constitute a part of the present application, and do not constitute an improper limitation of the present invention. In the drawings:

[0024] Figure 1 The branch diagram of the zucchini seedling selected from Example 1 of the present invention;

[0025] Figure 2 This is a diagram of the seedling hardening stage of Example 1 of the present invention;

[0026] Figure 3 This is a diagram of the daily management phase of Comparative Example 1 of the present invention;

[0027] Figure 4 The figure is a growth comparison diagram of the surviving cutting seedlings of the control group and the experimental group in Example 1 of the present invention;

[0028] Figure 5 This is a comparison diagram of the roots of the surviving cutting seedlings in the control group and the experimental group in Example 1 of the present invention;

[0029] Figure 6 This is a growth diagram of the flowering period of the chicory cuttings seedlings according to Example 1 of the present invention. DETAILED DESCRIPTION

[0030] The present invention will be described in detail below in conjunction with specific embodiments. The exemplary embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention. In the following examples, the 'Zhoujie No. 1' variety of winter melon was selected and the following method was used to carry out cutting seedling experiments.

[0031] Example 1

[0032] S1. The seedling matrix was thoroughly disinfected with a 1000-fold carbendazim aqueous solution and then loaded into a 32-hole seedling tray with a diameter of 6 cm and a depth of 5 cm for standby use. The seedling matrix was purchased from Hangzhou Jinhai Agricultural Technology Co., Ltd., and produced a gold No. 3 seedling matrix;

[0033] S2. Select a 2-3 leaf zucchini seedling, disinfect the scissors with a 75% volume concentration of ethanol aqueous solution, and then use the scissors to cut off the main branch of the zucchini seedling below the root, and remove some leaves on the upper part of the zucchini seedling, leaving one leaf and a growth point, with a length of 15-20 cm, as cuttings for future use; the cuttings are divided into two groups, one group is a control group with 18 replicates, and the other group is an experimental group with 21 replicates;

[0034] S3. The lower 3-5 cm portion of the experimental group cuttings obtained in step S2 was immersed in a pretreatment solution and taken out after 60 minutes. The pretreatment solution was mixed with an aqueous solution of glucose at a concentration of 8 g / L and an aqueous solution of indolebutyric acid at a concentration of 50 mg / L in a volume ratio of 1:1;

[0035] S4. The cuttings of the experimental group and the control group after the treatment in step S3 are inserted into the seedling medium in the seedling tray obtained in step S1, and the cutting depth is 3-4 cm;

[0036] S5. Build an arch shed, water the seedling tray after cutting in step S4 and place it in the arch shed, cover the arch shed with film and shade net, and carry out heat preservation, moisture retention, shade and hardening (the temperature in the arch shed is 20-35°C, and the relative humidity of the air is ≥80%); after 4 days of hardening, start semi-ventilation (lift two-thirds of the film on one side of the arch shed for ventilation); after 7 days of hardening, shade around noon (time is 9 am - 4 pm), and remove the shade net for the rest of the time; after 9 days of hardening, remove the film and shade Net, carry out daily management (daily natural light exposure, the temperature in the arch shed is below 35℃ during the day, the temperature in the arch shed is above 20℃ at night, and the seedling medium is kept moist); on the 3rd and 6th days after cutting, spray the cutting branches with 1000 times carbendazim aqueous solution for sterilization twice; 12 days after cutting, apply water-soluble fertilizer once, the water-soluble fertilizer is a special fertilizer for the growth period with a nitrogen, phosphorus and potassium content of 20-10-20 purchased from Hongyue Flower Co., Ltd., and diluted 500 times before application;

[0037] S6. After 19 days of cutting, adventitious roots grew at the bottom of the cutting branches, indicating that the cuttings survived. Plants that had grown 1-2 new leaves were selected as surviving plants, and the survival rate was determined.

[0038] The experimental results show that 20 out of 21 cuttings in the experimental group survived, with a survival rate of 95.24%, and 13 out of 18 cuttings in the control group survived, with a survival rate of 72.22%. It can be seen that the survival rate of the cuttings of the zucchini treated by the present invention is higher. Figure 4 (The left side is the control group, the right side is the experimental group) and Figure 5 (The left side is the control group, and the right side is the experimental group) It can be seen that the growth and rooting amount of the cuttings in the experimental group are better than those in the control group.

[0039] Example 2

[0040] S1. The seedling medium was thoroughly disinfected with an 800-fold solution of carbendazim, and then loaded into a 32-hole seedling tray with a diameter of 6 cm and a depth of 5 cm for standby use. The seedling medium was purchased from Hangzhou Jinhai Agricultural Technology Co., Ltd., and produced the gold No. 3 seedling medium;

[0041] S2. Select a 2-3 leaf zucchini seedling, sterilize the scissors with a 75% ethanol solution, and then cut off the main branch of the zucchini seedling below the root with scissors, make the cut surface flat, remove some leaves on the upper part of the zucchini seedling, and keep one leaf and growth point, with a length of 15-20 cm, as a cutting branch for standby, and set 40 cutting branches for replication;

[0042] S3. The lower 3-5 cm portion of the experimental group cuttings obtained in step S2 was immersed in a pretreatment solution and taken out after 50 minutes. The pretreatment solution was mixed with an aqueous solution of glucose at a concentration of 8 g / L and an aqueous solution of indolebutyric acid at a concentration of 50 mg / L in a volume ratio of 1:1;

[0043] S4. The cuttings of the experimental group and the control group after the treatment in step S3 are inserted into the seedling medium in the seedling tray obtained in step S1, and the cutting depth is 3-4 cm;

[0044] S5. Build an arch shed, water the seedling tray after cutting in step S4 and place it in the arch shed, cover the arch shed with film and shade net, and carry out heat preservation, moisture retention, shade and hardening (the temperature in the arch shed is 20-35°C, and the relative humidity of the air is ≥80%); after 3 days of hardening, start semi-ventilation (lift two-thirds of the film on one side of the arch shed for ventilation); after 5 days of hardening, shade around noon (time is 9 am - 4 pm), and remove the shade net for the rest of the time; after 8 days of hardening, remove the film and shade The sun net was used for daily management (daily natural light exposure, the temperature in the arch shed was below 35°C during the day and above 20°C at night, and the seedling medium was kept moist); on the 3rd and 6th days after cutting, the cutting branches were sprayed with 800 times carbendazim aqueous solution for sterilization twice; 10 days after cutting, water-soluble fertilizer was applied once, and the water-soluble fertilizer was a special fertilizer for the growth period with a nitrogen, phosphorus and potassium content of 20-10-20 purchased from Hongyue Flower Co., Ltd., which was diluted 500 times before application;

[0045] S6. After 16 days of cutting, adventitious roots grew at the bottom of the cutting branches, and the cuttings survived. Plants that had grown 1-2 new leaves were selected as surviving plants, and the survival rate was measured. The experimental results showed that 38 of the 40 cutting seedlings in Example 2 survived, with a survival rate of 95%.

[0046] Example 3

[0047] S1. The seedling medium was thoroughly disinfected with a 900-fold carbendazim aqueous solution and then loaded into a 32-hole seedling tray with a diameter of 6 cm and a depth of 5 cm for standby use. The seedling medium was purchased from Hangzhou Jinhai Agricultural Technology Co., Ltd., and produced the gold No. 3 seedling medium;

[0048] S2. Select 2-3 leaves of zucchini seedlings, disinfect the scissors with 75% ethanol solution, and then cut off the main branches of the zucchini seedlings below the root with scissors, make the cut surface flat, remove some leaves on the upper part of the zucchini seedlings, retain 2 leaves and growth points, and the length is 15-20cm, as cutting branches for standby, and set 29 cutting branches for replication;

[0049] S3. The lower 3-5 cm portion of the experimental group cuttings obtained in step S2 was immersed in a pretreatment solution and taken out after 70 minutes. The pretreatment solution was mixed with an aqueous solution of glucose at a concentration of 8 g / L and an aqueous solution of indolebutyric acid at a concentration of 50 mg / L in a volume ratio of 1:1;

[0050] S4. The cuttings of the experimental group and the control group after the treatment in step S3 are inserted into the seedling medium in the seedling tray obtained in step S1, and the cutting depth is 3-4 cm;

[0051] S5. Build an arch shed, water the seedling tray after cutting in step S4 and place it in the arch shed, cover the arch shed with film and shade net, and carry out heat preservation, moisture retention, shade and hardening (the temperature in the arch shed is 20-35°C, and the relative humidity of the air is ≥80%); after 4 days of hardening, start semi-ventilation (lift two-thirds of the film on one side of the arch shed for ventilation); after 6 days of hardening, shade around noon (time is 9 am - 4 pm), and remove the shade net for the rest of the time; after 8 days of hardening, remove the film and shade The sun net was used for daily management (daily natural light exposure, the temperature in the arch shed was below 35°C during the day and above 20°C at night, and the seedling medium was kept moist); on the 3rd and 6th days after cutting, the cutting branches were sprayed with a 900-fold carbendazim aqueous solution for sterilization twice; 11 days after cutting, water-soluble fertilizer was applied once, and the water-soluble fertilizer was a special fertilizer for the growth period with a nitrogen, phosphorus and potassium content of 20-10-20 purchased from Hongyue Flower Co., Ltd., which was diluted 500 times before application;

[0052] S6. After 18 days of cutting, adventitious roots grow at the bottom of the cutting branches, and the cuttings survive. Plants that have grown 1-2 new leaves are selected as surviving plants, and the survival rate is measured. The experimental results show that 28 out of 29 cutting seedlings in Example 2 survived, with a survival rate of 96.6%.

[0053] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for raising seedlings by cutting of zucchini, characterized in that: The following steps are involved: S1. Sterilize the seedling medium thoroughly with disinfectant and then place it in the seedling tray for later use; S2. Select 2-3 leaves of zucchini seedlings, cut off the main branches of zucchini seedlings below the root with scissors, remove some leaves on the upper part of zucchini seedlings, and retain 1-2 leaves and growth points as cuttings for standby; S3. The lower end of the cuttings obtained in step S2 is immersed in the pretreatment solution and removed after 50-70 minutes; S4. The cuttings processed in step S3 are inserted into the seedling medium in the seedling tray obtained in step S1; S5. Build an arch shed, water the seedling tray after the cutting in step S4 and place it in the arch shed, cover the arch shed with a film and a shade net for heat preservation, moisture retention, and shade hardening; after 3-4 days of hardening, start semi-ventilation; after 5-7 days of hardening, shade around noon, and remove the shade net for the rest of the time; after 8-9 days of hardening, remove the film and shade net for daily management; on the 3rd and 6th days after cutting, spray the cuttings with disinfectant twice; 10-12 days after cutting, apply water-soluble fertilizer once; S6. 16-19 days after cuttings, adventitious roots will grow at the bottom of the cuttings, and the cuttings will survive.

2. The method for raising seedlings of kiwifruit by cuttings according to claim 1, characterized in that: In the step S1, the seedling medium is a golden No. 3 seedling medium purchased from Hangzhou Jinhai Agricultural Technology Co., Ltd.; the disinfectant is an 800-1000 times carbendazim aqueous solution; and the seedling tray is a 32-hole seedling tray with a diameter of 6 cm and a depth of 5 cm.

3. The method for raising seedlings of kiwifruit by cuttings according to claim 1, characterized in that: In the step S2, the scissors are sterilized with an ethanol aqueous solution with a volume concentration of 75% before use; and the length of the cutting branches is 15-20 cm.

4. The method for raising seedlings of kiwifruit by cuttings according to claim 1, characterized in that: In step S3, the pretreatment solution is prepared by mixing an aqueous glucose solution with a concentration of 8 g / L and an aqueous indolebutyric acid solution with a concentration of 50 mg / L in a volume ratio of 1:1; and the immersion length of the cuttings is 3-5 cm.

5. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S4, the cutting depth of the cuttings in the seedling medium is 3-4 cm.

6. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S5, during the heat preservation, moisture retention, shading and seedling hardening treatment, the temperature in the arch shed is 20-35° C. and the relative humidity of the air is ≥80%.

7. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S5, the specific operation of semi-ventilation is: lifting two-thirds of the film on one side of the arch shed for ventilation.

8. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S5, the shading time around noon is from 9:00 to 16:

00.

9. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S5, the specific operations of daily management are: daily natural light irradiation, the temperature in the arch shed is below 35°C during the day, the temperature in the arch shed is above 20°C at night, and the seedling medium is kept moist.

10. The method for raising seedlings of zucchini by cuttings according to claim 1, characterized in that: In step S5, the disinfectant is an 800-1000 times carbendazim aqueous solution; the water-soluble fertilizer is a special fertilizer for the growing period with a nitrogen, phosphorus and potassium content of 20-10-20 purchased from Hongyue Flower Co., Ltd., which is diluted 500 times before application.

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