Alopecia-stopping and hair-growing composition based on platycladus orientalis compound extracting solution and preparation method thereof

Abstract

The invention belongs to the technical field of external compositions for skin, and particularly relates to an alopecia-preventing and hair-growing composition based on a platycladus orientalis compound extracting solution and a preparation method of the alopecia-preventing and hair-growing composition. The preparation method comprises the following steps: (1) mixing and crushing 10 parts by weight of cacumen biotae, 13-16 parts by weight of fructus forsythiae, 12-15 parts by weight of ligustrum lucidum fruits and 3-6 parts by weight of semen cuscutae to obtain a raw material A; (2) fermenting to obtain an extracting solution A; (3) mixing 18-22 parts of eclipta prostrata, 2-5 parts of radix sophorae flavescentis, 15-17 parts of felwort and 6-10 parts of dendrobium officinale, and crushing to obtain a raw material B; (4) mixing the raw material B and an ethanol water solution, heating, refluxing, extracting and filtering to obtain filtrate, and performing reduced-pressure rotary evaporation on the filtrate to recover ethanol to obtain residual liquid, namely an extracting solution B; (5) mixing the extracting solution A and the extracting solution B to obtain the alopecia-preventing and hair-growing composition based on the platycladus orientalis compound extracting solution. The composition disclosed by the invention has a good treatment effect on androgenetic alopecia.

Description

Technical Field

[0001] The invention belongs to the technical field of external skin compositions, and particularly relates to a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract and a preparation method thereof. Background Art

[0002] Hair loss, also known as hair loss or baldness, is a common health problem that can be caused by a variety of reasons and has different types. Androgenetic alopecia is the most common type of hair loss, usually related to genetics and hormone levels, especially more common in men, known as "male pattern baldness."

[0003] At present, there are mainly the following Western medicines for the treatment of androgenic alopecia: Minoxidil, which stimulates hair growth by dilating blood vessels, increasing blood flow to the scalp, and promoting the nutrient supply of hair follicles. Long-term use is required to maintain the effect; once use is stopped, new hair may fall off again. It may cause local side effects such as dry scalp, itching, redness and swelling. 2. Finasteride, an oral medication, inhibits 5α-reductase, reduces the production of dihydrotestosterone (DHT), and thus slows down the process of hair loss. It is mainly suitable for men, and women should use it with caution because it may cause serious side effects. It may cause side effects such as sexual dysfunction.

[0004] Chinese herbal extracts have many advantages in treating hair loss, and have fewer toxic side effects than chemical synthetic drugs. The theory of traditional Chinese medicine emphasizes the holistic concept, not only focusing on the site of hair loss, but also considering the overall condition of the body, such as qi and blood, liver and kidney, etc., to achieve the purpose of treatment by adjusting the body's functions. Therefore, Western medicines such as minoxidil and finasteride have been widely used in the treatment of androgenic alopecia, but they have certain limitations and side effects. In contrast, Chinese herbal extracts, with their advantages of naturalness, comprehensive conditioning, personalized treatment and less dependence, have made up for the shortcomings of Western medicine to a certain extent. However, the products currently on the market for androgenic alopecia are not ideal.

[0005] Therefore, there is an urgent need for a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract and a preparation method thereof. Summary of the invention

[0006] The purpose of the present invention is to provide a hair loss prevention and hair growth composition based on Platycladus orientalis compound extract and a preparation method thereof.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,

[0009] (1) 10 parts of Platycladus orientalis leaves, 13-16 parts of Forsythia suspensa, 12-15 parts of Ligustrum lucidum fruits, and 3-6 parts of Cuscuta australis were mixed and crushed to obtain a raw material A;

[0010] (2) mixing raw material A and water in a weight ratio of 1:(6-8) to obtain a mixture, adding Lactobacillus plantarum to the mixture, fermenting, sterilizing after the fermentation is complete, filtering, and obtaining an extract A;

[0011] (3) mixing 18-22 parts of Channa intestine, 2-5 parts of Sophora flavescens, 15-17 parts of Gentiana scabra, and 6-10 parts of Dendrobium officinale, and crushing them to obtain raw material B;

[0012] (4) mixing the raw material B and ethanol aqueous solution in a weight ratio of 1:(6-8), heating and refluxing for extraction, filtering to obtain a filtrate, and subjecting the filtrate to reduced pressure rotary evaporation to recover ethanol to obtain the remaining liquid as extract B;

[0013] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

[0014] The present invention can improve androgenic alopecia by selecting specific components and preparing a composition by specific proportions, wherein the main medicine is Platycladus orientalis leaf and Forsythia suspensa; the effects are antibacterial and anti-inflammatory, hair growth and blackening, and hair loss prevention. Mechanism of action: these medicinal materials have antibacterial and anti-inflammatory effects, can reduce scalp inflammation, and promote hair growth. Assistant medicine: Cuscuta chinensis and Ligustrum lucidum fruit; the effects are tonic and nourish the liver and kidney. Mechanism of action: these medicinal materials help to replenish qi and blood, nourish the liver and kidney, thereby improving the nutritional supply of hair and enhancing the growth ability of hair. Adjuvant medicine: Channa intestine and Sophora flavescens; the effects are to activate blood circulation. Mechanism of action: these medicinal materials can promote blood circulation, increase blood supply to the scalp, thereby improving the nutritional status of hair follicles and promoting hair growth. Enhancing medicine: Gentiana scabra and Dendrobium officinale; the effects are to calm the nerves and nourish blood. Mechanism of action: these medicinal materials help to calm the nerves and nourish blood, reduce the impact of mental stress on hair, and improve overall health status, thereby indirectly promoting hair growth. Through the combination of these Chinese medicinal materials, the scalp environment can be improved in many aspects, inflammation can be reduced, blood circulation can be promoted, and nutrition can be supplemented, thereby effectively treating androgenic alopecia.

[0015] Furthermore, in step (1), the powder is crushed to a size less than 100 mesh.

[0016] Furthermore, the fermentation conditions in step (2) are 35-40° C. for 10-12 hours.

[0017] Furthermore, in step (2), 0.22 μm filter membrane is used for filtration.

[0018] Furthermore, in step (3), the powder is crushed to a size less than 300 mesh.

[0019] Furthermore, the volume concentration of the ethanol aqueous solution in step (4) is 55-60%.

[0020] Furthermore, the time of heating reflux extraction in step (4) is 2-3h.

[0021] Furthermore, in step (4), 0.22 μm filter membrane is used for filtration.

[0022] The present invention can improve the α-reductase inhibition rate by dividing the raw materials into two groups, and performing fermentation and alcohol extraction respectively, thereby effectively treating androgenic alopecia. Ingredients such as Platycladus orientalis leaves, Forsythia suspensa, Ligustrum lucidum, and Cuscuta australis may contain precursor compounds that require biotransformation to better release active substances. By using plant lactobacillus for fermentation, some compounds in these ingredients can be promoted to be converted into more easily absorbed or more active forms. Ingredients such as Channa intestines, Sophora flavescens, Gentiana scabra, and Dendrobium candidum may be rich in fat-soluble or heat-stable active ingredients, and such ingredients are more suitable for extraction by heating and refluxing with ethanol-water solution.

[0023] The present invention also provides a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract prepared by the preparation method.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. The present invention can improve androgenic alopecia by selecting specific components and preparing a composition in a specific ratio.

[0026] 2. The present invention can improve the α-reductase inhibition rate by dividing the raw materials into two groups for fermentation and alcohol extraction respectively, thereby effectively treating androgenic alopecia. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is the acute skin toxicity test report.

[0028] Figure 2 This is the test report for 5α-reductase inhibition test.

[0029] Figure 3 This is a before and after comparison picture of Volunteer No. 1.

[0030] Figure 4 This is a before and after comparison picture of Volunteer 2.

[0031] Figure 5 This is a before and after comparison picture of volunteer No. 3.

[0032] Figure 6This is a before and after comparison picture of volunteer No. 4. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The raw materials used in the following examples of the present invention are all commercially available products:

[0035] Lactobacillus plantarum, product number CCTCC LB 2008168, was purchased from China Center for Type Culture Collection.

[0036] Lactobacillus bulgaricus, product number CCTCC CB 20082296, was purchased from China Center for Type Culture Collection.

[0037] Example 1

[0038] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,

[0039] (1) 10 parts of Platycladus orientalis leaves, 14 parts of Forsythia suspensa, 13 parts of Ligustrum lucidum fruits, and 5 parts of Cuscuta australis were mixed and crushed to less than 100 meshes to obtain raw material A;

[0040] (2) Mixing raw material A and water in a weight ratio of 1:7 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 38° C. for 11 hours. After the fermentation is completed, sterilizing, filtering with a 0.22 μm filter membrane, and obtaining an extract A;

[0041] (3) 20 parts of Channa intestine, 4 parts of Sophora flavescens, 16 parts of Gentiana scabra, and 7 parts of Dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;

[0042] (4) mixing the raw material B and the ethanol aqueous solution with a volume concentration of 58% in a weight ratio of 1:7, heating and refluxing for extraction for 2.5 hours, filtering with a 0.22 μm filter membrane to obtain a filtrate, and reevaporating the filtrate under reduced pressure to recover ethanol, and obtaining the remaining liquid as the extract B;

[0043] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

[0044] Example 2

[0045] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,

[0046] (1) 10 parts of Platycladus orientalis leaves, 13 parts of Forsythia suspensa, 15 parts of Ligustrum lucidum fruits, and 3 parts of Cuscuta australis were mixed and crushed to less than 100 meshes to obtain raw material A;

[0047] (2) Mixing raw material A and water in a weight ratio of 1:6 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 40° C. for 10 h. After the fermentation is completed, sterilizing, filtering with a 0.22 μm filter membrane, and obtaining an extract A;

[0048] (3) 22 parts of Channa intestine, 2 parts of Sophora flavescens, 17 parts of Gentiana scabra, and 6 parts of Dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;

[0049] (4) mixing the raw material B and the ethanol aqueous solution with a volume concentration of 55% in a weight ratio of 1:6, heating and refluxing for extraction for 3 hours, filtering with a 0.22 μm filter membrane to obtain a filtrate, and reevaporating the filtrate under reduced pressure to recover the ethanol, and obtaining the remaining liquid as the extract B;

[0050] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

[0051] Example 3

[0052] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,

[0053] (1) 10 parts of Platycladus orientalis leaves, 16 parts of Forsythia suspensa, 12 parts of Ligustrum lucidum fruits, and 6 parts of Cuscuta australis were mixed and crushed to less than 100 meshes to obtain raw material A;

[0054] (2) Mixing raw material A and water in a weight ratio of 1:8 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 35° C. for 12 hours. After the fermentation is completed, sterilizing, filtering with a 0.22 μm filter membrane, and obtaining an extract A;

[0055] (3) 18 parts of Channa intestine, 5 parts of Sophora flavescens, 15 parts of Gentiana scabra, and 10 parts of Dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;

[0056] (4) mixing the raw material B and the ethanol aqueous solution with a volume concentration of 60% in a weight ratio of 1:8, heating and refluxing for extraction for 2 h, filtering with a 0.22 μm filter membrane to obtain a filtrate, and reevaporating the filtrate under reduced pressure to recover ethanol, and obtaining the remaining liquid as the extract B;

[0057] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

[0058] Comparative Example 1

[0059] The difference between this comparative example and Example 1 is that 10 parts of Platycladus orientalis leaves, 13 parts of Forsythia suspensa, 15 parts of Ligustrum lucidum fruits, and 3 parts of Cuscuta seeds are replaced by 13 parts of Platycladus orientalis leaves, 10 parts of Forsythia suspensa, 3 parts of Ligustrum lucidum fruits, and 15 parts of Cuscuta seeds.

[0060] Comparative Example 2

[0061] The difference between this comparative example and Example 1 is that the Ligustrum lucidum fruit is replaced by Scutellaria baicalensis.

[0062] Comparative Example 3

[0063] The difference between this comparative example and Example 1 is that 20 parts of Channa Intestines, 4 parts of Sophora flavescens, 16 parts of Gentiana scabra and 7 parts of Dendrobium officinale are replaced by 12 parts of Channa Intestines, 12 parts of Sophora flavescens, 7 parts of Gentiana scabra and 16 parts of Dendrobium officinale.

[0064] Comparative Example 4

[0065] The difference between this comparative example and Example 1 is that Gentiana is replaced by Eucommia ulmoides.

[0066] Comparative Example 5

[0067] The difference between this comparative example and Example 1 is that Lactobacillus plantarum is replaced by Lactobacillus bulgaricus.

[0068] Comparative Example 6

[0069] The difference between this comparative example and Example 1 is: a preparation method of a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,

[0070] (1) 10 parts of Platycladus orientalis leaves, 14 parts of Forsythia suspensa, 13 parts of Ligustrum lucidum fruits, 5 parts of Cuscuta seeds, 20 parts of Channa cheilica, 4 parts of Sophora flavescens, 16 parts of Gentiana scabra, and 7 parts of Dendrobium officinale were mixed and crushed to less than 100 mesh to obtain a raw material;

[0071] (2) The raw materials in a weight ratio of 1:7 and an ethanol aqueous solution with a volume concentration of 58% were mixed, heated under reflux for extraction for 2.5 hours, and filtered using a 0.22 μm filter membrane to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

[0072] Performance Testing

[0073] The hair loss prevention and hair growth compositions based on Platycladus orientalis compound extract prepared in Examples 1-3 and Comparative Examples 1-6 were used as samples for performance testing.

[0074] 1. According to Chapter 6 of the "Technical Specification for Safety of Cosmetics" (2015 edition), the samples were subjected to multiple skin irritation tests, with 4 New Zealand rabbits (ordinary grade, female, weighing 2.0-2.5kg) in each group, and the animal certificate number is NO.44411600012896.

[0075] The results showed that the reaction scores of erythema and edema of each animal in each group of samples at 14 days were all 0, indicating that the compositions prepared in Examples 1-3 and Comparative Examples 1-6 were non-irritating.

[0076] 2. According to Figure 1 The samples were subjected to acute skin toxicity tests using the method described in the previous article. The results showed that no poisoning symptoms or deaths occurred in the animals in each group within 14 days of exposure. The weight of the animals was normal, and no abnormalities were found in the gross autopsies of the animals after the end of the test. This indicates that the compositions prepared in Examples 1-3 and Comparative Examples 1-6 are non-toxic. Figure 1 This is the test report of the sample in Example 1.

[0077] 3. The 5α-reductase inhibition test is mainly used to evaluate whether the activity of 5α-reductase can be effectively inhibited. This enzyme is one of the key factors in androgenic alopecia. Androgenic alopecia is related to increased levels of dihydrotestosterone (DHT) in the body, and DHT is converted from testosterone under the action of 5α-reductase. Therefore, by inhibiting 5α-reductase, the production of DHT can be reduced, thereby slowing down the process of hair loss. Figure 2 The 5α-reductase inhibition assay was performed using the method described in .

[0078] The results are shown in Table 1. Figure 2 This is the test report of the sample in Example 1.

[0079] Table 1. 5α-reductase inhibition test results

[0080]

[0081]

[0082] From the above performance test results, it can be seen that the anti-hair loss and hair growth compositions based on the Platycladus orientalis compound extract of Examples 1-2 are non-irritating, non-toxic, and have good α-reductase inhibition effects, especially the comprehensive performance of Example 1 is the most outstanding.

[0083] However, the comparative example does not adopt the necessary technical solution, resulting in that it is significantly inferior to the embodiment in the corresponding performance test.

[0084] In Comparative Example 1, the ratio of Platycladus orientalis leaves, Forsythia suspensa, Ligustrum lucidum fruit and Cuscuta australis was changed, and it can be seen that the inhibitory effect decreased, which proves that the components of the specific formula are fermented and play a synergistic role with each other during the fermentation process, and more abundant active components can be extracted to inhibit α-reductase.

[0085] In Comparative Example 2, Ligustrum lucidum fruit was replaced with Scutellaria baicalensis, and it can be seen that the inhibitory effect decreased, indicating that the prescriptions of the present invention have a synergistic effect with each other, and the effect decreases if the raw materials are replaced randomly.

[0086] In Comparative Example 3, the ratio of Channa intestine, Sophora flavescens, Gentiana scabra and Dendrobium officinale was changed, and it can be seen that the inhibitory effect decreased, which proves that more active components can be extracted by extracting components of a specific formula to inhibit α-reductase.

[0087] In Comparative Example 4, gentian was replaced with eucommia, and it can be seen that the inhibitory effect decreased, indicating that the prescriptions of the present invention have synergistic effects with each other, and the effect decreases if the raw materials are replaced randomly.

[0088] In Comparative Example 5, different bacteria were used for fermentation, and it can be seen that the inhibitory effect decreased, which proves that the active components prepared by the present invention using specific bacteria for fermentation are more conducive to inhibiting α-reductase.

[0089] In Comparative Example 6, all components were mixed and then subjected to alcohol extraction. It can be seen that the inhibitory effect decreased, indicating that the extraction rate of active components in this extraction method is low.

[0090] 4. The anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract prepared in Example 1 was mixed with water in a volume ratio of 1:1 and used. Four volunteers with androgenic alopecia were randomly selected, and 2 mL was evenly applied to the entire scalp once a day for 3 consecutive months. The results are shown in Table 1. Figure 3-6 .

[0091] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, characterized in that: The preparation method comprises the following steps: by weight, (1) 10 parts of Platycladus orientalis leaves, 13-16 parts of Forsythia suspensa, 12-15 parts of Ligustrum lucidum fruits, and 3-6 parts of Cuscuta australis were mixed and crushed to obtain a raw material A; (2) mixing raw material A and water to obtain a mixture, adding Lactobacillus plantarum to the mixture, fermenting, sterilizing after the fermentation is complete, filtering, and obtaining an extract A; (3) mixing 18-22 parts of Channa intestine, 2-5 parts of Sophora flavescens, 15-17 parts of Gentiana scabra, and 6-10 parts of Dendrobium officinale, and crushing them to obtain raw material B; (4) mixing the raw material B and the ethanol aqueous solution, heating and refluxing for extraction, filtering to obtain a filtrate, and subjecting the filtrate to reduced pressure rotary evaporation to recover ethanol to obtain the remaining liquid as the extract B; (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.

2. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (2), raw material A and water are mixed in a weight ratio of 1:(6-8).

3. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (1), the powder is crushed to a size less than 100 mesh.

4. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: The fermentation conditions in step (2) are 35-40° C. for 10-12 hours.

5. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (2), a 0.22 μm filter membrane is used for filtration.

6. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (3), the powder is crushed to a size less than 300 mesh.

7. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: The volume concentration of the ethanol aqueous solution in step (4) is 55-60%.

8. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: The time of heating reflux extraction in step (4) is 2-3 hours.

9. The method for preparing the anti-hair loss and hair growth composition based on Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (4), a 0.22 μm filter membrane is used for filtration.

10. A hair loss prevention and hair growth composition based on Platycladus orientalis compound extract prepared by the preparation method according to any one of claims 1 to 9.

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